FvH4_1g10930

Thiosulfate sulfurtransferase 16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005733
Physical Location & Seq
Forward (+)
5954861 .. 5956855
1995 bp
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UTR
Exon/CDS
Intron
FvH4_1g10930.t2

Sequence Viewer

Length: 543 bp
ATGACTTCTCTGTCCACCTCTTGCTTAACTGGTCCTGTACTTCGACTCCCGTCTCTTCTTTCTCCTCAAAATCCTAATCAGAGGTCCTTGTTGTCACTGACTCGGAACCCGGATCGAATTCGTGGCAATCGGATTGTATGCAAGAACTTAAGTGTCTGTCCGAAGGCTAGTCTGAGAGGGAATTTGGAGGTCGCCGGACTTCCAACGTCGGTGCCGGTGCGTGTGGCTCACGAGCTTCTTCTGGCCGGACACCATTATTTGGATGTCAGGACTCCTGAAGAGTTTAGTGCAGGGCATCCTTCTGGTGCTGTTAACATTCCTTACTTGTACAGAGTTGGATCAGGGATGTCAAAGAATCCAGAGTTTGTGAAGGAGGTTTCATCACATTTCAGAAAACATGATGAAATTATTGTTGGATGCCAGCTTGGGAAAAGATCAATGATGGCTGCAACTGATCTTGTAGCTGCTGGTTTCACTGGGATTACAGACATGGCCGGTGGGTATGCTACTTGGACTCAAAATGGACTTCCAACAGAGTTGTGA

Protein Analysis

181

Amino Acids

19.41

Weight (kDa)

9.23

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 85 - 142 4.2e-07 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
AccB1I GGYRCC 1 cut(s) 211
AccB7I CCANNNNNTGG 1 cut(s) 259
AclWI GGATC 2 cut(s) 120, 346
AcoI YGGCCR 2 cut(s) 243, 492
AcsI RAATTY 2 cut(s) 117, 181
AcuI CTGAAG 1 cut(s) 297
AfaI GTAC 2 cut(s) 39, 329
AfiI CCNNNNNNNGG 1 cut(s) 259
AflII CTTAAG 1 cut(s) 148
AjuI GAANNNNNNNTTGG 2 cut(s) 396, 428
AluBI AGCT 3 cut(s) 235, 424, 464
AluI AGCT 3 cut(s) 235, 424, 464
Alw26I GTCTC 1 cut(s) 57
AlwI GGATC 2 cut(s) 120, 346
AoxI GGCC 2 cut(s) 243, 492
ApeKI GCWGC 2 cut(s) 446, 464
ApoI RAATTY 2 cut(s) 117, 181
AspS9I GGNCC 2 cut(s) 32, 84
AsuC2I CCSGG 1 cut(s) 110
AvaII GGWCC 2 cut(s) 32, 84
BanI GGYRCC 1 cut(s) 211
BauI CACGAG 1 cut(s) 230
BbvI GCAGC 2 cut(s) 433, 451
BccI CCATC 1 cut(s) 436
BcnI CCSGG 1 cut(s) 110
BcoDI GTCTC 1 cut(s) 57
BfaI CTAG 1 cut(s) 168
BfrI CTTAAG 1 cut(s) 148
BisI GCNGC 2 cut(s) 447, 465
BlsI GCNGC 2 cut(s) 448, 466
Bme1390I CCNGG 1 cut(s) 110
Bme18I GGWCC 2 cut(s) 32, 84
BmgT120I GGNCC 2 cut(s) 32, 84
BmiI GGNNCC 2 cut(s) 107, 213
BmrFI CCNGG 1 cut(s) 110
BmrI ACTGGG 1 cut(s) 486
BmsI GCATC 2 cut(s) 304, 407
BmuI ACTGGG 1 cut(s) 486
BoxI GACNNNNGTC 1 cut(s) 49
BpuMI CCSGG 1 cut(s) 110
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse118I RCCGGY 2 cut(s) 214, 494
Bse1I ACTGG 2 cut(s) 34, 481
BseGI GGATG 4 cut(s) 268, 295, 351, 422
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMII CTCAG 1 cut(s) 164
BseNI ACTGG 2 cut(s) 34, 481
BseRI GAGGAG 1 cut(s) 54
BseXI GCAGC 2 cut(s) 433, 451
BsgI GTGCAG 1 cut(s) 309
BshFI GGCC 2 cut(s) 245, 494
BshNI GGYRCC 1 cut(s) 211
BsiSI CCGG 5 cut(s) 110, 195, 215, 246, 495
BslI CCNNNNNNNGG 1 cut(s) 259
BsmAI GTCTC 1 cut(s) 57
BsmBI CGTCTC 1 cut(s) 57
BsnI GGCC 2 cut(s) 245, 494
Bsp1407I TGTACA 1 cut(s) 327
Bsp143I GATC 4 cut(s) 112, 338, 434, 454
BspANI GGCC 2 cut(s) 245, 494
BspCNI CTCAG 1 cut(s) 165
BspLI GGNNCC 2 cut(s) 107, 213
BspPI GGATC 2 cut(s) 120, 346
BspT107I GGYRCC 1 cut(s) 211
BspTI CTTAAG 1 cut(s) 148
BsrFI RCCGGY 2 cut(s) 214, 494
BsrGI TGTACA 1 cut(s) 327
BsrI ACTGG 2 cut(s) 34, 481
BssAI RCCGGY 2 cut(s) 214, 494
BssMI GATC 4 cut(s) 112, 338, 434, 454
BssSI CACGAG 1 cut(s) 230
Bst2BI CACGAG 1 cut(s) 230
Bst6I CTCTTC 2 cut(s) 60, 273
BstAFI CTTAAG 1 cut(s) 148
BstAUI TGTACA 1 cut(s) 327
BstC8I GCNNGC 1 cut(s) 422
BstDEI CTNAG 1 cut(s) 173
BstF5I GGATG 4 cut(s) 268, 295, 351, 422
BstKTI GATC 4 cut(s) 115, 341, 437, 457
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 4 cut(s) 112, 338, 434, 454
BstPAI GACNNNNGTC 1 cut(s) 49
BstSCI CCNGG 1 cut(s) 108
BstV1I GCAGC 2 cut(s) 433, 451
BsuRI GGCC 2 cut(s) 245, 494
BtsCI GGATG 4 cut(s) 268, 295, 351, 422
BtsIMutI CAGTG 2 cut(s) 95, 474
Cac8I GCNNGC 1 cut(s) 422
Cfr10I RCCGGY 2 cut(s) 214, 494
Cfr13I GGNCC 2 cut(s) 32, 84
Csp6I GTAC 2 cut(s) 38, 328
CviAII CATG 2 cut(s) 398, 490
CviJI RGCY 8 cut(s) 167, 227, 235, 245, 424, 446, 464, 494
CviKI_1 RGCY 8 cut(s) 167, 227, 235, 245, 424, 446, 464, 494
CviQI GTAC 2 cut(s) 38, 328
DdeI CTNAG 1 cut(s) 173
DpnI GATC 4 cut(s) 114, 340, 436, 456
DpnII GATC 4 cut(s) 112, 338, 434, 454
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 2 cut(s) 243, 492
Eam1104I CTCTTC 2 cut(s) 60, 273
EarI CTCTTC 2 cut(s) 60, 273
Eco47I GGWCC 2 cut(s) 32, 84
Eco57I CTGAAG 1 cut(s) 297
EcoO109I RGGNCCY 1 cut(s) 84
EcoRI GAATTC 1 cut(s) 117
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 2 cut(s) 401, 493
FaiI YATR 4 cut(s) 139, 399, 491, 504
FatI CATG 2 cut(s) 397, 489
Fnu4HI GCNGC 2 cut(s) 447, 465
FokI GGATG 4 cut(s) 275, 282, 358, 429
Fsp4HI GCNGC 2 cut(s) 447, 465
FspBI CTAG 1 cut(s) 168
GluI GCNGC 2 cut(s) 447, 465
HaeIII GGCC 2 cut(s) 245, 494
HapII CCGG 5 cut(s) 110, 195, 215, 246, 495
Hin1II CATG 2 cut(s) 401, 493
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HinfI GANTC 5 cut(s) 45, 100, 271, 355, 514
HpaI GTTAAC 1 cut(s) 313
HpaII CCGG 5 cut(s) 110, 195, 215, 246, 495
Hpy166II GTNNAC 2 cut(s) 15, 313
Hpy188I TCNGA 6 cut(s) 81, 105, 132, 162, 174, 392
Hpy188III TCNNGA 4 cut(s) 230, 268, 275, 359
Hpy8I GTNNAC 2 cut(s) 15, 313
Hpy99I CGWCG 1 cut(s) 211
HpyAV CCTTC 3 cut(s) 157, 309, 364
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 3 cut(s) 141, 290, 449
HpyF3I CTNAG 1 cut(s) 173
HpySE526I ACGT 1 cut(s) 206
Hsp92II CATG 2 cut(s) 401, 493
KspAI GTTAAC 1 cut(s) 313
Kzo9I GATC 4 cut(s) 112, 338, 434, 454
Lsp1109I GCAGC 2 cut(s) 433, 451
LweI GCATC 2 cut(s) 304, 407
MaeI CTAG 1 cut(s) 168
MaeII ACGT 1 cut(s) 206
MaeIII GTNAC 1 cut(s) 93
MalI GATC 4 cut(s) 114, 340, 436, 456
MboI GATC 4 cut(s) 112, 338, 434, 454
MboII GAAGA 3 cut(s) 47, 230, 290
MluCI AATT 3 cut(s) 117, 181, 405
MlyI GAGTC 4 cut(s) 39, 94, 265, 508
MmeI TCCRAC 3 cut(s) 227, 316, 394
MnlI CCTC 6 cut(s) 28, 75, 75, 170, 181, 367
MseI TTAA 3 cut(s) 26, 149, 312
MspCI CTTAAG 1 cut(s) 148
MspI CCGG 5 cut(s) 110, 195, 215, 246, 495
MspR9I CCNGG 1 cut(s) 110
NciI CCSGG 1 cut(s) 110
NdeII GATC 4 cut(s) 112, 338, 434, 454
NlaIII CATG 2 cut(s) 401, 493
NlaIV GGNNCC 2 cut(s) 107, 213
NmuCI GTSAC 1 cut(s) 93
PfeI GAWTC 1 cut(s) 355
PflMI CCANNNNNTGG 1 cut(s) 259
PkrI GCNGC 2 cut(s) 448, 466
PleI GAGTC 4 cut(s) 39, 94, 265, 508
PpsI GAGTC 4 cut(s) 39, 94, 265, 508
PpuMI RGGWCCY 1 cut(s) 84
PshAI GACNNNNGTC 1 cut(s) 49
Psp5II RGGWCCY 1 cut(s) 84
PspN4I GGNNCC 2 cut(s) 107, 213
PspPI GGNCC 2 cut(s) 32, 84
PspPPI RGGWCCY 1 cut(s) 84
RsaI GTAC 2 cut(s) 39, 329
RsaNI GTAC 2 cut(s) 38, 328
SaqAI TTAA 3 cut(s) 26, 149, 312
SatI GCNGC 2 cut(s) 447, 465
Sau3AI GATC 4 cut(s) 112, 338, 434, 454
Sau96I GGNCC 2 cut(s) 32, 84
SchI GAGTC 4 cut(s) 39, 94, 265, 508
ScrFI CCNGG 1 cut(s) 110
SetI ASST 8 cut(s) 20, 86, 192, 209, 237, 378, 426, 466
SfaNI GCATC 2 cut(s) 304, 407
SinI GGWCC 2 cut(s) 32, 84
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
Sse9I AATT 3 cut(s) 117, 181, 405
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 1 cut(s) 108
TaiI ACGT 1 cut(s) 209
TaqI TCGA 2 cut(s) 43, 115
TasI AATT 3 cut(s) 117, 181, 405
TatI WGTACW 2 cut(s) 37, 327
TfiI GAWTC 1 cut(s) 355
Tru1I TTAA 3 cut(s) 26, 149, 312
Tru9I TTAA 3 cut(s) 26, 149, 312
TscAI CASTG 2 cut(s) 102, 481
TseFI GTSAC 1 cut(s) 93
TseI GCWGC 2 cut(s) 446, 464
Tsp45I GTSAC 1 cut(s) 93
TspDTI ATGAA 2 cut(s) 369, 417
TspRI CASTG 2 cut(s) 102, 481
Van91I CCANNNNNTGG 1 cut(s) 259
Vha464I CTTAAG 1 cut(s) 148
VpaK11BI GGWCC 2 cut(s) 32, 84
XapI RAATTY 2 cut(s) 117, 181
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.