MD15G1236700.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010681
Physical Location & Seq
Reverse (-)
19460429 .. 19466980
6552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1236700.v1.1.491

Sequence Viewer

Length: 1722 bp
ATGGCTATAGACGAAGACGTGGAGAGCTGCGGCAGCAGAGCGGCTCTGTCGTCGCAGGCGCAGCCCCGCCACCACCGCCAAAAATTGGAAGTGTACAACGAGGTGCTCCGACGAATACAAGATTATAATTTTGAGGAGGCTAATCTTCCTGGATTTGATGATCAGCTTTGGCTTCACTTCAATCGGTTACCTGCTCGTTATGCATTGGATGTGAATGTGGAGAGGGCAGAAGATGTTATAACACATAAGAGATTGCTGCAATTTGCAGAAGACCCTGCTAACCAACCCGCCTTTGAAGTTCGCTCTGTGCAGGTTTGTCCTGTCTTCAATGGAAATTCTGTTGATTCTGTCCATTCAGATTCTTCAATGAAAGAAGATGCACAAAGCACTTTCAATTATTCTAATAGACAAGGGATTCATGCACCTCCCACCTTTGGTTCATCTCCTACTCTTGACGCACTTGCACTTCAAGCCAATAGATACCATGTTGAAGATGGAGGTAGTGCCATGAGTGCAACACCAGTCTTTTCGCGGCCTATGCATGAGATCACGTTTTCAACAGTTGACAAGCCCAAACTTCTTAGTCAGTTAACTTCATTACTTGCTGATATTGGACTGAATATTCAAGAAGCTCATGCTTTCTCCACTGTTGATGGATTTTCTCTTGATGTTTTTGTGGTTGATGGGTGGAAATATGAGGAAACCGAGGAGCTCAAAAGTGTGCTAGAAAAGGAAATTTCAAAATTTAAGGATCAATCTTGGTCAAAACAACATCCAATCGCTGCTATTGGTGAGGACAATCAAACAAGGAATGAATCCTTCCCCAGTAGCATAGAAATACCAACTGATGGAACAGATGTCTGGGAGATTGATACAAGGCAGCTGAAATTTGAGAACAAAGTTGGATCTGGGTCTTTCGGTGATCTGTACAGAGGCACATATTGTACTCAGGAAGTTGCCATAAAAGTTCTCAAACCTGAGCGTGTCAATGCAGAGATGCTGAGGGATTTTTCACAGGAAGTTTACATTTTGAGGAAGATTCGGCACAAGAATGTTGTGCAATTTATCGGTGCATGTACACGACCTCCAAACCTATGTATTGTGACAGAGTTTATGTCCAAAGGAAGTGTGTACGATTTTCTGCATAAGCACAAGGGTGTGTTTAAGCTTCCATCTCTACTCAAAGTAGCAATTGACGTTTCCAAGGGAATGAACTATTTGCACCAAAATAATATAATCCACAGGGATCTTAAGACTGCCAATCTTCTTATGGATGAACATGAAGTTGTTAAGGTTGCTGATTTTGGGGTTGCCAGAGTGCAGACTCAATCTGGAGTGATGACAGCTGAAACTGGTACATACCGCTGGATGGCTCCTGAGGTCATCGAACACAAACCATATGATCACAAGGCAGATGTTTTCAGTTTTGGAATAGTTCTGTGGGAGCTTCTAACTGGACAACTCCCCTACTCATCCTTGACACCGTTACAAGCAGCAGTAGGTGTGGTGCAAAAGAGCCTACGACCTACAATTCCCAAGACCACTCACCCAAGATTCGCCGAACTGCTTGAGAGATGCTGGCAACAAGACCCGACTCAAAGGCCCCCCTTCTCCGATATTGTAGAAATCCTACAGAATTTGGCCAAAGAGGTTGGAAATGAGGGAGAAGATCGACGCAGATCTGGTGGTTTTTTCTCGGCACTTAGATGGGGCAATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

574

Amino Acids

64.81

Weight (kDa)

5.76

Isoelectric Point (pI)

52.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 295 - 544 2.3e-74 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 297 - 541 2.7e-61 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010681)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17700 AT4G35780
fragaria_vesca FvH4_1g10940 FvH4_1g10940 FvH4_1g10940
malus_domestica MD02G1122500.v1.1 MD15G1236700.v1.1
prunus_persica Prupe.7G176000_v2.0.a1
pyrus_communis pycom02g09550 pycom15g21040
rosa_chinensis RchiOBHm_Chr2g0098201
rosa_laevigata RLG00000016776
rosa_multiflora Rmu_sc0009178.1_g000003
rosa_roxburghii Rroxscaffold_2G00144160
rosa_rugosa Rorug02G0071100 Rorug02G0071200.1
rosa_samantha Rh2AG118300 Rh2BG121300 Rh2DG123000
rosa_wichuraiana Rw2G009270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 126, 239
Acc36I ACCTGC 2 cut(s) 199, 301
AccB7I CCANNNNNTGG 2 cut(s) 85, 848
AccBSI CCGCTC 1 cut(s) 41
AccII CGCG 1 cut(s) 532
AciI CCGC 7 cut(s) 30, 41, 67, 76, 288, 532, 1363
AclWI GGATC 3 cut(s) 759, 913, 1254
AcoI YGGCCR 1 cut(s) 1641
AcsI RAATTY 5 cut(s) 334, 735, 743, 887, 1636
AfaI GTAC 6 cut(s) 95, 929, 946, 1078, 1133, 1357
AfiI CCNNNNNNNGG 4 cut(s) 85, 434, 848, 1369
AflII CTTAAG 1 cut(s) 1250
AjiI CACGTC 1 cut(s) 19
AjnI CCWGG 1 cut(s) 148
AleI CACNNNNGTG 1 cut(s) 1155
AluBI AGCT 8 cut(s) 27, 166, 632, 712, 883, 1168, 1346, 1447
AluI AGCT 8 cut(s) 27, 166, 632, 712, 883, 1168, 1346, 1447
Alw21I GWGCWC 2 cut(s) 108, 714
AlwI GGATC 3 cut(s) 759, 913, 1254
AoxI GGCC 3 cut(s) 533, 1601, 1641
ApeKI GCWGC 7 cut(s) 27, 33, 61, 256, 782, 880, 1493
ApoI RAATTY 5 cut(s) 334, 735, 743, 887, 1636
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 1 cut(s) 1602
AsuHPI GGTGA 3 cut(s) 803, 932, 1538
AxyI CCTNAGG 1 cut(s) 1377
BalI TGGCCA 1 cut(s) 1643
BanII GRGCYC 1 cut(s) 714
BbsI GAAGAC 3 cut(s) 21, 276, 316
Bbv12I GWGCWC 2 cut(s) 108, 714
BbvCI CCTCAGC 1 cut(s) 1001
BbvI GCAGC 7 cut(s) 14, 45, 73, 243, 769, 892, 1505
BccI CCATC 7 cut(s) 488, 647, 677, 842, 1180, 1363, 1701
BciT130I CCWGG 1 cut(s) 150
BclI TGATCA 2 cut(s) 160, 1402
BfaI CTAG 1 cut(s) 725
BfmI CTRYAG 2 cut(s) 6, 1631
BfrI CTTAAG 1 cut(s) 1250
BfuAI ACCTGC 2 cut(s) 199, 301
BglII AGATCT 1 cut(s) 1679
Bme1390I CCNGG 1 cut(s) 150
BmgBI CACGTC 1 cut(s) 19
BmgT120I GGNCC 1 cut(s) 1602
BmiI GGNNCC 2 cut(s) 1374, 1604
BmrFI CCNGG 1 cut(s) 150
BmrI ACTGGG 1 cut(s) 819
BmsI GCATC 3 cut(s) 367, 987, 1565
BmuI ACTGGG 1 cut(s) 819
BpiI GAAGAC 3 cut(s) 21, 276, 316
BpmI CTGGAG 1 cut(s) 1353
Bpu10I CCTNAGC 2 cut(s) 978, 1001
BpuEI CTTGAG 1 cut(s) 1589
BsaJI CCNNGG 2 cut(s) 705, 1203
BsaXI ACNNNNNCTCC 2 cut(s) 1069, 1099
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 434, 848, 1369
Bse1I ACTGG 4 cut(s) 521, 825, 1357, 1459
Bse21I CCTNAGG 1 cut(s) 1377
BseBI CCWGG 1 cut(s) 150
BseDI CCNNGG 2 cut(s) 705, 1203
BseGI GGATG 5 cut(s) 214, 772, 1279, 1374, 1472
BseLI CCNNNNNNNGG 4 cut(s) 85, 434, 848, 1369
BseMII CTCAG 4 cut(s) 962, 969, 992, 1368
BseNI ACTGG 4 cut(s) 521, 825, 1357, 1459
BseRI GAGGAG 2 cut(s) 149, 722
BseXI GCAGC 7 cut(s) 14, 45, 73, 243, 769, 892, 1505
BsgI GTGCAG 2 cut(s) 329, 1340
Bsh1236I CGCG 1 cut(s) 532
BshFI GGCC 3 cut(s) 535, 1603, 1643
BsiHKAI GWGCWC 2 cut(s) 108, 714
BslI CCNNNNNNNGG 4 cut(s) 85, 434, 848, 1369
BsnI GGCC 3 cut(s) 535, 1603, 1643
Bsp1286I GDGCHC 2 cut(s) 108, 714
Bsp1407I TGTACA 3 cut(s) 93, 927, 1076
Bsp143I GATC 9 cut(s) 160, 546, 751, 905, 922, 1246, 1402, 1669, 1679
BspACI CCGC 7 cut(s) 30, 41, 67, 76, 288, 532, 1363
BspANI GGCC 3 cut(s) 535, 1603, 1643
BspCNI CTCAG 4 cut(s) 961, 970, 993, 1369
BspFNI CGCG 1 cut(s) 532
BspLI GGNNCC 2 cut(s) 1374, 1604
BspMI ACCTGC 2 cut(s) 199, 301
BspPI GGATC 3 cut(s) 759, 913, 1254
BspTI CTTAAG 1 cut(s) 1250
BsrBI CCGCTC 1 cut(s) 41
BsrGI TGTACA 3 cut(s) 93, 927, 1076
BsrI ACTGG 4 cut(s) 521, 825, 1357, 1459
BssECI CCNNGG 2 cut(s) 705, 1203
BssMI GATC 9 cut(s) 160, 546, 751, 905, 922, 1246, 1402, 1669, 1679
BssT1I CCWWGG 1 cut(s) 1203
Bst2UI CCWGG 1 cut(s) 150
Bst4CI ACNGT 3 cut(s) 562, 649, 1485
BstAFI CTTAAG 1 cut(s) 1250
BstAUI TGTACA 3 cut(s) 93, 927, 1076
BstC8I GCNNGC 2 cut(s) 57, 1580
BstDEI CTNAG 6 cut(s) 581, 948, 978, 1001, 1377, 1703
BstEII GGTNACC 1 cut(s) 186
BstF5I GGATG 5 cut(s) 214, 772, 1279, 1374, 1472
BstFNI CGCG 1 cut(s) 532
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 9 cut(s) 163, 549, 754, 908, 925, 1249, 1405, 1672, 1682
BstMBI GATC 9 cut(s) 160, 546, 751, 905, 922, 1246, 1402, 1669, 1679
BstMWI GCNNNNNNNGC 7 cut(s) 33, 61, 75, 200, 470, 512, 538
BstNI CCWGG 1 cut(s) 150
BstNSI RCATGY 1 cut(s) 1077
BstPI GGTNACC 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 148
BstSFI CTRYAG 2 cut(s) 6, 1631
BstUI CGCG 1 cut(s) 532
BstV1I GCAGC 7 cut(s) 14, 45, 73, 243, 769, 892, 1505
BstV2I GAAGAC 3 cut(s) 21, 276, 316
BstX2I RGATCY 3 cut(s) 905, 1246, 1679
BstYI RGATCY 3 cut(s) 905, 1246, 1679
Bsu36I CCTNAGG 1 cut(s) 1377
BsuRI GGCC 3 cut(s) 535, 1603, 1643
BtrI CACGTC 1 cut(s) 19
BtsCI GGATG 5 cut(s) 214, 772, 1279, 1374, 1472
BtsIMutI CAGTG 2 cut(s) 645, 1717
BveI ACCTGC 2 cut(s) 199, 301
Cac8I GCNNGC 2 cut(s) 57, 1580
CfoI GCGC 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 1602
CseI GACGC 2 cut(s) 464, 1683
Csp6I GTAC 6 cut(s) 94, 928, 945, 1077, 1132, 1356
CviAII CATG 7 cut(s) 419, 485, 508, 542, 635, 1074, 1280
CviQI GTAC 6 cut(s) 94, 928, 945, 1077, 1132, 1356
DdeI CTNAG 6 cut(s) 581, 948, 978, 1001, 1377, 1703
DpnI GATC 9 cut(s) 162, 548, 753, 907, 924, 1248, 1404, 1671, 1681
DpnII GATC 9 cut(s) 160, 546, 751, 905, 922, 1246, 1402, 1669, 1679
EaeI YGGCCR 1 cut(s) 1641
Ecl136II GAGCTC 1 cut(s) 712
Eco130I CCWWGG 1 cut(s) 1203
Eco24I GRGCYC 1 cut(s) 714
Eco53kI GAGCTC 1 cut(s) 712
Eco81I CCTNAGG 1 cut(s) 1377
Eco91I GGTNACC 1 cut(s) 186
EcoICRI GAGCTC 1 cut(s) 712
EcoO109I RGGNCCY 1 cut(s) 1602
EcoO65I GGTNACC 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 148
EcoT14I CCWWGG 1 cut(s) 1203
EcoT22I ATGCAT 2 cut(s) 205, 543
EcoT38I GRGCYC 1 cut(s) 714
ErhI CCWWGG 1 cut(s) 1203
FaeI CATG 7 cut(s) 422, 488, 511, 545, 638, 1077, 1283
FatI CATG 7 cut(s) 418, 484, 507, 541, 634, 1073, 1279
FauI CCCGC 2 cut(s) 74, 295
FauNDI CATATG 1 cut(s) 1399
FbaI TGATCA 2 cut(s) 160, 1402
FokI GGATG 5 cut(s) 221, 759, 1286, 1381, 1459
FriOI GRGCYC 1 cut(s) 714
FspBI CTAG 1 cut(s) 725
GlaI GCGC 1 cut(s) 60
GsuI CTGGAG 1 cut(s) 1353
HaeIII GGCC 3 cut(s) 535, 1603, 1643
HgaI GACGC 2 cut(s) 464, 1683
HhaI GCGC 1 cut(s) 61
Hin1II CATG 7 cut(s) 422, 488, 511, 545, 638, 1077, 1283
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HincII GTYRAC 2 cut(s) 565, 591
HindII GTYRAC 2 cut(s) 565, 591
HindIII AAGCTT 1 cut(s) 1166
HinfI GANTC 8 cut(s) 344, 359, 415, 815, 1039, 1324, 1554, 1594
HpaI GTTAAC 1 cut(s) 591
HphI GGTGA 3 cut(s) 803, 932, 1538
Hpy166II GTNNAC 6 cut(s) 94, 565, 591, 1024, 1079, 1132
Hpy188I TCNGA 3 cut(s) 110, 358, 1615
Hpy188III TCNNGA 6 cut(s) 452, 626, 665, 950, 1332, 1376
Hpy8I GTNNAC 6 cut(s) 94, 565, 591, 1024, 1079, 1132
Hpy99I CGWCG 3 cut(s) 55, 114, 1677
HpyAV CCTTC 2 cut(s) 829, 1618
HpyCH4III ACNGT 3 cut(s) 562, 649, 1485
HpyCH4IV ACGT 3 cut(s) 18, 551, 1197
HpyF10VI GCNNNNNNNGC 7 cut(s) 33, 61, 75, 200, 470, 512, 538
HpyF3I CTNAG 6 cut(s) 581, 948, 978, 1001, 1377, 1703
HpySE526I ACGT 3 cut(s) 18, 551, 1197
Hsp92II CATG 7 cut(s) 422, 488, 511, 545, 638, 1077, 1283
HspAI GCGC 1 cut(s) 59
Ksp22I TGATCA 2 cut(s) 160, 1402
KspAI GTTAAC 1 cut(s) 591
Kzo9I GATC 9 cut(s) 160, 546, 751, 905, 922, 1246, 1402, 1669, 1679
LmnI GCTCC 4 cut(s) 111, 709, 1378, 1444
Lsp1109I GCAGC 7 cut(s) 14, 45, 73, 243, 769, 892, 1505
LweI GCATC 3 cut(s) 367, 987, 1565
MaeI CTAG 1 cut(s) 725
MaeII ACGT 3 cut(s) 18, 551, 1197
MaeIII GTNAC 3 cut(s) 186, 1102, 1485
MalI GATC 9 cut(s) 162, 548, 753, 907, 924, 1248, 1404, 1671, 1681
MbiI CCGCTC 1 cut(s) 41
MboI GATC 9 cut(s) 160, 546, 751, 905, 922, 1246, 1402, 1669, 1679
MfeI CAATTG 1 cut(s) 1191
MflI RGATCY 3 cut(s) 905, 1246, 1679
MhlI GDGCHC 2 cut(s) 108, 714
MlsI TGGCCA 1 cut(s) 1643
MluNI TGGCCA 1 cut(s) 1643
MlyI GAGTC 2 cut(s) 1318, 1588
MmeI TCCRAC 3 cut(s) 133, 883, 1633
Mox20I TGGCCA 1 cut(s) 1643
Mph1103I ATGCAT 2 cut(s) 205, 543
MscI TGGCCA 1 cut(s) 1643
MseI TTAA 5 cut(s) 590, 747, 1164, 1251, 1290
MslI CAYNNNNRTG 3 cut(s) 1050, 1155, 1705
Msp20I TGGCCA 1 cut(s) 1643
MspA1I CMGCKG 3 cut(s) 883, 1346, 1365
MspCI CTTAAG 1 cut(s) 1250
MspR9I CCNGG 1 cut(s) 150
MunI CAATTG 1 cut(s) 1191
MvaI CCWGG 1 cut(s) 150
MvnI CGCG 1 cut(s) 532
MwoI GCNNNNNNNGC 7 cut(s) 33, 61, 75, 200, 470, 512, 538
NdeI CATATG 1 cut(s) 1399
NdeII GATC 9 cut(s) 160, 546, 751, 905, 922, 1246, 1402, 1669, 1679
NlaIII CATG 7 cut(s) 422, 488, 511, 545, 638, 1077, 1283
NlaIV GGNNCC 2 cut(s) 1374, 1604
NmeAIII GCCGAG 1 cut(s) 1676
NmuCI GTSAC 1 cut(s) 1102
NsiI ATGCAT 2 cut(s) 205, 543
NspI RCATGY 1 cut(s) 1077
OliI CACNNNNGTG 1 cut(s) 1155
PfeI GAWTC 6 cut(s) 344, 359, 415, 815, 1039, 1554
PflMI CCANNNNNTGG 2 cut(s) 85, 848
PfoI TCCNGGA 1 cut(s) 148
PleI GAGTC 2 cut(s) 1318, 1588
PpsI GAGTC 2 cut(s) 1318, 1588
PsiI TTATAA 2 cut(s) 126, 239
Psp124BI GAGCTC 1 cut(s) 714
Psp6I CCWGG 1 cut(s) 148
PspEI GGTNACC 1 cut(s) 186
PspGI CCWGG 1 cut(s) 148
PspN4I GGNNCC 2 cut(s) 1374, 1604
PspPI GGNCC 1 cut(s) 1602
PsuI RGATCY 3 cut(s) 905, 1246, 1679
PvuII CAGCTG 2 cut(s) 883, 1346
RsaI GTAC 6 cut(s) 95, 929, 946, 1078, 1133, 1357
RsaNI GTAC 6 cut(s) 94, 928, 945, 1077, 1132, 1356
RseI CAYNNNNRTG 3 cut(s) 1050, 1155, 1705
SacI GAGCTC 1 cut(s) 714
SaqAI TTAA 5 cut(s) 590, 747, 1164, 1251, 1290
Sau3AI GATC 9 cut(s) 160, 546, 751, 905, 922, 1246, 1402, 1669, 1679
Sau96I GGNCC 1 cut(s) 1602
SchI GAGTC 2 cut(s) 1318, 1588
ScrFI CCNGG 1 cut(s) 150
SduI GDGCHC 2 cut(s) 108, 714
SfaNI GCATC 3 cut(s) 367, 987, 1565
SfcI CTRYAG 2 cut(s) 6, 1631
SmiMI CAYNNNNRTG 3 cut(s) 1050, 1155, 1705
SmlI CTYRAG 2 cut(s) 1250, 1568
SmoI CTYRAG 2 cut(s) 1250, 1568
SsiI CCGC 7 cut(s) 30, 41, 67, 76, 288, 532, 1363
SspI AATATT 1 cut(s) 622
SspMI CTAG 1 cut(s) 725
SstI GAGCTC 1 cut(s) 714
StyD4I CCNGG 1 cut(s) 148
StyI CCWWGG 1 cut(s) 1203
TaaI ACNGT 3 cut(s) 562, 649, 1485
TaiI ACGT 3 cut(s) 21, 554, 1200
TaqI TCGA 2 cut(s) 1386, 1672
TatI WGTACW 4 cut(s) 93, 927, 944, 1076
TauI GCSGC 3 cut(s) 33, 44, 535
TfiI GAWTC 6 cut(s) 344, 359, 415, 815, 1039, 1554
Tru1I TTAA 5 cut(s) 590, 747, 1164, 1251, 1290
Tru9I TTAA 5 cut(s) 590, 747, 1164, 1251, 1290
TscAI CASTG 1 cut(s) 652
TseFI GTSAC 1 cut(s) 1102
TseI GCWGC 7 cut(s) 27, 33, 61, 256, 782, 880, 1493
Tsp45I GTSAC 1 cut(s) 1102
TspDTI ATGAA 8 cut(s) 383, 407, 429, 585, 828, 1226, 1290, 1296
TspRI CASTG 1 cut(s) 652
Van91I CCANNNNNTGG 2 cut(s) 85, 848
Vha464I CTTAAG 1 cut(s) 1250
XapI RAATTY 5 cut(s) 334, 735, 743, 887, 1636
XceI RCATGY 1 cut(s) 1077
XcmI CCANNNNNNNNNTGG 2 cut(s) 491, 1267
XspI CTAG 1 cut(s) 725
Zsp2I ATGCAT 2 cut(s) 205, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.