MD02G1124700.v1.1

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014043
Physical Location & Seq
Forward (+)
10148695 .. 10150363
1669 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1124700.v1.1.491

Sequence Viewer

Length: 957 bp
ATGGCTTTCAATCTAGCATTCTTGAGCTCAATCTTCATCATCCTTTCGGTATTTTCTGCAGTGACTAACGCGTTGAGCTCGAATTACTACGATAAGAGCTGTCCCAATGTCGATCAAATCGTTTCTAATGCCGTCAAGCAGGCGACATCCAACGACAGAACTGTCCCCGCTGCTCTACTCCGGATGCACTTCCACGACTGCTTCATTAGGGGCTGTGATGCATCTGTGCTGTTGAACTCCAAGGGAGGCAACAAAGCTGAGAAAGATGGGCCACCAAATATTTCTCTGCATGCGTTTTATGTCATTGACAATGCAAAGAAACAAGTGGAGGCTTCTTGCCCTGGTGTCGTTTCATGTGCTGATATCTTGGCTTTGGCAGCAAGGGATGCTGTTGTGCAATCCGGAGGTCCGAACTGGGCTGTGCCGAAAGGACGAAAGGATGGAAGAACATCGAAGGCTAGCGAGACCGTGCAGCTGCCGGCTCCAACCTTCAACATATCTCAACTGCAGCAGAGCTTCTCGCAGAGAGGTCTGTCCTTGAACGACCTGGTGGCTCTTTCAGGAGGGCACACTCTAGGGTTCTCCCACTGCTCATCGTTCCAAAACAGAATCCACAACTTCAACGCCACACACGACTTTGATCCAACACTGAACCCGTCCTTTGCTGCAAATTTGAAGAATACGTGTCCCATCAAGAACAGGCCGAAGAATGCCGGTGCCACCATGGATCCTTCTACAACCACCTTTGATAATACATACTTCAAGTTGATCCTCCAAGGGAAGAGCTTGTTTTCTTCGGATCAAGCTCTGCTCAGTTTTCCGAAGACTAAAGGTTTGGTTACTAAGTTTGCCTCCTCAAAACAAGCGTTCTTAGACGCTTTCGTGAATTCTATGATAAAGATGAGCAGCATTAATGGTGGGCAAGAGATCAGGAAGGACTGCAGGGTAGTAAATTAA

Protein Analysis

319

Amino Acids

34.31

Weight (kDa)

9.26

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 41 - 285 5.4e-85 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014043)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42180
fragaria_vesca FvH4_1g11220
malus_domestica MD02G1124700.v1.1 MD15G1238800.v1.1
prunus_persica Prupe.7G173700_v2.0.a1 Prupe.7G173700_v2.0.a1
pyrus_communis pycom02g09740
rosa_chinensis RchiOBHm_Chr2g0098631
rosa_laevigata RLG00000016819
rosa_multiflora Rmu_sc0002776.1_g000019
rosa_roxburghii Rroxscaffold_2G00143830
rosa_rugosa Rorug02G0073800
rosa_samantha Rh2AG121600 Rh2BG125100 Rh2CG126100 Rh2DG127200
rosa_wichuraiana Rw0G004160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
AccB1I GGYRCC 1 cut(s) 716
AccII CGCG 1 cut(s) 71
AccIII TCCGGA 2 cut(s) 180, 401
AciI CCGC 1 cut(s) 168
AclWI GGATC 5 cut(s) 635, 722, 735, 763, 807
AcsI RAATTY 2 cut(s) 670, 886
AflIII ACRYGT 2 cut(s) 69, 683
AgsI TTSAA 7 cut(s) 10, 235, 493, 541, 622, 676, 763
AjnI CCWGG 2 cut(s) 340, 546
AluBI AGCT 8 cut(s) 27, 78, 99, 257, 475, 516, 786, 806
AluI AGCT 8 cut(s) 27, 78, 99, 257, 475, 516, 786, 806
Alw21I GWGCWC 2 cut(s) 29, 80
Alw26I GTCTC 1 cut(s) 458
AlwI GGATC 5 cut(s) 635, 722, 735, 763, 807
Aor13HI TCCGGA 2 cut(s) 180, 401
AoxI GGCC 2 cut(s) 269, 701
ApeKI GCWGC 7 cut(s) 170, 377, 472, 475, 508, 665, 906
ApoI RAATTY 2 cut(s) 670, 886
ArsI GACNNNNNNTTYG 2 cut(s) 817, 849
AseI ATTAAT 1 cut(s) 912
AspS9I GGNCC 2 cut(s) 269, 407
AsuNHI GCTAGC 1 cut(s) 458
AvaII GGWCC 1 cut(s) 407
BaeGI GKGCMC 1 cut(s) 570
BamHI GGATCC 1 cut(s) 727
BanI GGYRCC 1 cut(s) 716
BanII GRGCYC 2 cut(s) 29, 80
BbsI GAAGAC 1 cut(s) 830
Bbv12I GWGCWC 2 cut(s) 29, 80
BbvI GCAGC 7 cut(s) 157, 389, 462, 484, 520, 652, 918
BccI CCATC 3 cut(s) 260, 434, 698
BceAI ACGGC 1 cut(s) 116
BciT130I CCWGG 2 cut(s) 342, 548
BcoDI GTCTC 1 cut(s) 458
BfaI CTAG 3 cut(s) 14, 459, 575
BfmI CTRYAG 3 cut(s) 57, 506, 940
BisI GCNGC 7 cut(s) 171, 378, 473, 476, 509, 666, 907
BlsI GCNGC 7 cut(s) 172, 379, 474, 477, 510, 667, 908
Bme1390I CCNGG 2 cut(s) 342, 548
Bme18I GGWCC 1 cut(s) 407
BmgT120I GGNCC 2 cut(s) 269, 407
BmiI GGNNCC 3 cut(s) 483, 718, 729
BmrFI CCNGG 2 cut(s) 342, 548
BmrI ACTGGG 1 cut(s) 424
BmsI GCATC 4 cut(s) 174, 208, 230, 376
BmtI GCTAGC 1 cut(s) 462
BmuI ACTGGG 1 cut(s) 424
BpiI GAAGAC 1 cut(s) 830
BpuEI CTTGAG 1 cut(s) 43
BsaAI YACGTR 1 cut(s) 684
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 4 cut(s) 240, 340, 723, 775
BsaWI WCCGGW 2 cut(s) 180, 401
Bse118I RCCGGY 2 cut(s) 478, 713
Bse1I ACTGG 1 cut(s) 419
BseAI TCCGGA 2 cut(s) 180, 401
BseBI CCWGG 2 cut(s) 342, 548
BseDI CCNNGG 4 cut(s) 240, 340, 723, 775
BseGI GGATG 5 cut(s) 39, 146, 189, 391, 445
BseMII CTCAG 2 cut(s) 249, 826
BseNI ACTGG 1 cut(s) 419
BseRI GAGGAG 1 cut(s) 844
BseSI GKGCMC 1 cut(s) 570
BseXI GCAGC 7 cut(s) 157, 389, 462, 484, 520, 652, 918
BsgI GTGCAG 1 cut(s) 491
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 2 cut(s) 271, 703
BshNI GGYRCC 1 cut(s) 716
BsiHKAI GWGCWC 2 cut(s) 29, 80
BsiSI CCGG 4 cut(s) 181, 402, 479, 714
BslFI GGGAC 3 cut(s) 87, 149, 672
BsmAI GTCTC 1 cut(s) 458
BsmFI GGGAC 3 cut(s) 87, 149, 672
BsmI GAATGC 2 cut(s) 17, 715
BsnI GGCC 2 cut(s) 271, 703
Bso31I GGTCTC 1 cut(s) 458
Bsp1286I GDGCHC 3 cut(s) 29, 80, 570
Bsp13I TCCGGA 2 cut(s) 180, 401
Bsp143I GATC 6 cut(s) 112, 640, 727, 768, 799, 927
Bsp19I CCATGG 1 cut(s) 723
BspACI CCGC 1 cut(s) 168
BspANI GGCC 2 cut(s) 271, 703
BspCNI CTCAG 2 cut(s) 250, 825
BspEI TCCGGA 2 cut(s) 180, 401
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 3 cut(s) 483, 718, 729
BspMAI CTGCAG 3 cut(s) 61, 510, 944
BspOI GCTAGC 1 cut(s) 462
BspPI GGATC 5 cut(s) 635, 722, 735, 763, 807
BspQI GCTCTTC 1 cut(s) 776
BspT107I GGYRCC 1 cut(s) 716
BspTNI GGTCTC 1 cut(s) 458
BsrFI RCCGGY 2 cut(s) 478, 713
BsrI ACTGG 1 cut(s) 419
BssAI RCCGGY 2 cut(s) 478, 713
BssECI CCNNGG 4 cut(s) 240, 340, 723, 775
BssMI GATC 6 cut(s) 112, 640, 727, 768, 799, 927
BssT1I CCWWGG 3 cut(s) 240, 723, 775
Bst2UI CCWGG 2 cut(s) 342, 548
Bst4CI ACNGT 2 cut(s) 163, 469
Bst6I CTCTTC 1 cut(s) 776
BstAPI GCANNNNNTGC 1 cut(s) 386
BstBAI YACGTR 1 cut(s) 684
BstC8I GCNNGC 4 cut(s) 141, 291, 460, 480
BstDEI CTNAG 4 cut(s) 258, 812, 843, 871
BstDSI CCRYGG 1 cut(s) 723
BstF5I GGATG 5 cut(s) 39, 146, 189, 391, 445
BstFNI CGCG 1 cut(s) 71
BstKTI GATC 6 cut(s) 115, 643, 730, 771, 802, 930
BstMAI GTCTC 1 cut(s) 458
BstMBI GATC 6 cut(s) 112, 640, 727, 768, 799, 927
BstMWI GCNNNNNNNGC 2 cut(s) 377, 386
BstNI CCWGG 2 cut(s) 342, 548
BstNSI RCATGY 1 cut(s) 293
BstSCI CCNGG 2 cut(s) 340, 546
BstSFI CTRYAG 3 cut(s) 57, 506, 940
BstSLI GKGCMC 1 cut(s) 570
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 7 cut(s) 157, 389, 462, 484, 520, 652, 918
BstV2I GAAGAC 1 cut(s) 830
BstX2I RGATCY 1 cut(s) 727
BstYI RGATCY 1 cut(s) 727
BsuRI GGCC 2 cut(s) 271, 703
BtgI CCRYGG 1 cut(s) 723
BtsCI GGATG 5 cut(s) 39, 146, 189, 391, 445
BtsI GCAGTG 2 cut(s) 66, 586
BtsIMutI CAGTG 3 cut(s) 66, 586, 647
Cac8I GCNNGC 4 cut(s) 141, 291, 460, 480
Cfr10I RCCGGY 2 cut(s) 478, 713
Cfr13I GGNCC 2 cut(s) 269, 407
CseI GACGC 1 cut(s) 884
CsiI ACCWGGT 1 cut(s) 546
CviAII CATG 3 cut(s) 290, 354, 724
DdeI CTNAG 4 cut(s) 258, 812, 843, 871
DpnI GATC 6 cut(s) 114, 642, 729, 770, 801, 929
DpnII GATC 6 cut(s) 112, 640, 727, 768, 799, 927
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
Eam1104I CTCTTC 1 cut(s) 776
EarI CTCTTC 1 cut(s) 776
Ecl136II GAGCTC 2 cut(s) 27, 78
Eco130I CCWWGG 3 cut(s) 240, 723, 775
Eco24I GRGCYC 2 cut(s) 29, 80
Eco31I GGTCTC 1 cut(s) 458
Eco32I GATATC 1 cut(s) 364
Eco47I GGWCC 1 cut(s) 407
Eco53kI GAGCTC 2 cut(s) 27, 78
EcoICRI GAGCTC 2 cut(s) 27, 78
EcoRI GAATTC 1 cut(s) 886
EcoRII CCWGG 2 cut(s) 340, 546
EcoRV GATATC 1 cut(s) 364
EcoT14I CCWWGG 3 cut(s) 240, 723, 775
EcoT22I ATGCAT 1 cut(s) 223
EcoT38I GRGCYC 2 cut(s) 29, 80
ErhI CCWWGG 3 cut(s) 240, 723, 775
FaeI CATG 3 cut(s) 293, 357, 727
FaiI YATR 7 cut(s) 291, 300, 355, 497, 725, 757, 893
FaqI GGGAC 3 cut(s) 87, 149, 672
FatI CATG 3 cut(s) 289, 353, 723
FauI CCCGC 1 cut(s) 175
Fnu4HI GCNGC 7 cut(s) 171, 378, 473, 476, 509, 666, 907
FokI GGATG 5 cut(s) 26, 133, 196, 398, 452
FriOI GRGCYC 2 cut(s) 29, 80
Fsp4HI GCNGC 7 cut(s) 171, 378, 473, 476, 509, 666, 907
FspBI CTAG 3 cut(s) 14, 459, 575
GluI GCNGC 7 cut(s) 171, 378, 473, 476, 509, 666, 907
HaeIII GGCC 2 cut(s) 271, 703
HapII CCGG 4 cut(s) 181, 402, 479, 714
HgaI GACGC 1 cut(s) 884
Hin1II CATG 3 cut(s) 293, 357, 727
HinfI GANTC 1 cut(s) 609
HpaII CCGG 4 cut(s) 181, 402, 479, 714
Hpy188I TCNGA 3 cut(s) 411, 799, 822
Hpy188III TCNNGA 7 cut(s) 22, 181, 402, 561, 694, 883, 931
HpyAV CCTTC 4 cut(s) 448, 499, 741, 928
HpyCH4III ACNGT 2 cut(s) 163, 469
HpyCH4IV ACGT 1 cut(s) 683
HpyF10VI GCNNNNNNNGC 2 cut(s) 377, 386
HpyF3I CTNAG 4 cut(s) 258, 812, 843, 871
HpySE526I ACGT 1 cut(s) 683
Hsp92II CATG 3 cut(s) 293, 357, 727
Kpn2I TCCGGA 2 cut(s) 180, 401
KroI GCCGGC 1 cut(s) 478
KroNI GCCGGC 1 cut(s) 480
Kzo9I GATC 6 cut(s) 112, 640, 727, 768, 799, 927
LguI GCTCTTC 1 cut(s) 776
LmnI GCTCC 1 cut(s) 487
Lsp1109I GCAGC 7 cut(s) 157, 389, 462, 484, 520, 652, 918
LweI GCATC 4 cut(s) 174, 208, 230, 376
MabI ACCWGGT 1 cut(s) 546
MaeI CTAG 3 cut(s) 14, 459, 575
MaeII ACGT 1 cut(s) 683
MaeIII GTNAC 2 cut(s) 61, 838
MalI GATC 6 cut(s) 114, 642, 729, 770, 801, 929
MboI GATC 6 cut(s) 112, 640, 727, 768, 799, 927
MboII GAAGA 7 cut(s) 25, 456, 688, 718, 786, 793, 835
MflI RGATCY 1 cut(s) 727
MhlI GDGCHC 3 cut(s) 29, 80, 570
MluCI AATT 4 cut(s) 82, 670, 886, 952
MluI ACGCGT 1 cut(s) 69
MmeI TCCRAC 3 cut(s) 174, 509, 668
MnlI CCTC 8 cut(s) 239, 322, 398, 521, 557, 782, 862, 865
Mph1103I ATGCAT 1 cut(s) 223
MroI TCCGGA 2 cut(s) 180, 401
MroNI GCCGGC 1 cut(s) 478
MseI TTAA 2 cut(s) 912, 955
MspA1I CMGCKG 2 cut(s) 170, 475
MspI CCGG 4 cut(s) 181, 402, 479, 714
MspR9I CCNGG 2 cut(s) 342, 548
Mva1269I GAATGC 2 cut(s) 17, 715
MvaI CCWGG 2 cut(s) 342, 548
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 2 cut(s) 377, 386
NaeI GCCGGC 1 cut(s) 480
NcoI CCATGG 1 cut(s) 723
NdeII GATC 6 cut(s) 112, 640, 727, 768, 799, 927
NgoMIV GCCGGC 1 cut(s) 478
NheI GCTAGC 1 cut(s) 458
NlaIII CATG 3 cut(s) 293, 357, 727
NlaIV GGNNCC 3 cut(s) 483, 718, 729
NmuCI GTSAC 1 cut(s) 61
NsiI ATGCAT 1 cut(s) 223
NspI RCATGY 1 cut(s) 293
PaeI GCATGC 1 cut(s) 293
PciSI GCTCTTC 1 cut(s) 776
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 630
PctI GAATGC 2 cut(s) 17, 715
PdiI GCCGGC 1 cut(s) 480
PfeI GAWTC 1 cut(s) 609
PkrI GCNGC 7 cut(s) 172, 379, 474, 477, 510, 667, 908
Ppu21I YACGTR 1 cut(s) 684
PshBI ATTAAT 1 cut(s) 912
Psp124BI GAGCTC 2 cut(s) 29, 80
Psp6I CCWGG 2 cut(s) 340, 546
PspGI CCWGG 2 cut(s) 340, 546
PspN4I GGNNCC 3 cut(s) 483, 718, 729
PspPI GGNCC 2 cut(s) 269, 407
PstI CTGCAG 3 cut(s) 61, 510, 944
PsuI RGATCY 1 cut(s) 727
PvuII CAGCTG 1 cut(s) 475
SacI GAGCTC 2 cut(s) 29, 80
SapI GCTCTTC 1 cut(s) 776
SaqAI TTAA 2 cut(s) 912, 955
SatI GCNGC 7 cut(s) 171, 378, 473, 476, 509, 666, 907
Sau3AI GATC 6 cut(s) 112, 640, 727, 768, 799, 927
Sau96I GGNCC 2 cut(s) 269, 407
ScrFI CCNGG 2 cut(s) 342, 548
SduI GDGCHC 3 cut(s) 29, 80, 570
SexAI ACCWGGT 1 cut(s) 546
SfaNI GCATC 4 cut(s) 174, 208, 230, 376
SfcI CTRYAG 3 cut(s) 57, 506, 940
SinI GGWCC 1 cut(s) 407
SmlI CTYRAG 1 cut(s) 22
SmoI CTYRAG 1 cut(s) 22
SphI GCATGC 1 cut(s) 293
Sse9I AATT 4 cut(s) 82, 670, 886, 952
SsiI CCGC 1 cut(s) 168
SspI AATATT 1 cut(s) 280
SspMI CTAG 3 cut(s) 14, 459, 575
SstI GAGCTC 2 cut(s) 29, 80
StyD4I CCNGG 2 cut(s) 340, 546
StyI CCWWGG 3 cut(s) 240, 723, 775
TaaI ACNGT 2 cut(s) 163, 469
TaiI ACGT 1 cut(s) 686
TaqI TCGA 3 cut(s) 80, 111, 452
TasI AATT 4 cut(s) 82, 670, 886, 952
TfiI GAWTC 1 cut(s) 609
Tru1I TTAA 2 cut(s) 912, 955
Tru9I TTAA 2 cut(s) 912, 955
TscAI CASTG 3 cut(s) 66, 593, 654
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 7 cut(s) 170, 377, 472, 475, 508, 665, 906
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 3 cut(s) 25, 193, 342
TspRI CASTG 3 cut(s) 66, 593, 654
VpaK11BI GGWCC 1 cut(s) 407
VspI ATTAAT 1 cut(s) 912
XapI RAATTY 2 cut(s) 670, 886
XceI RCATGY 1 cut(s) 293
XspI CTAG 3 cut(s) 14, 459, 575
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.