MD02G1136800.v1.1

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011678
Physical Location & Seq
Reverse (-)
11414494 .. 11418663
4170 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1136800.v1.1.491

Sequence Viewer

Length: 2907 bp
ATGGCGCCAGGTTTAAGGTCGCGCAGCAACAAGCGGTCATCTTCGTCGAATTCTTACGCTTCGACTGTGACGATTATGGTGTTCATAGCAGTGTGCGTTTTGGCTGTGTGGATGCTCAACTACTCTAACTCAGGAGTCCCCCAAACCTCCACAAGTGCTCGAACTGCCACGATCTCCTCCTCAGACGATGCTGCCTCGGACCTAGTGATCACCAAAAACACAAACAATTACGACAACAACAAGAACAATAATAATAATGAAAAACCGAAGGCGTTTGAAGATAACCCGGGCGATCTCCCCGACGATGCCATCAAATCCGATGATCTGCTGAGCAATTCAACAAAGGGTAGCGGTAGTAGTACTACTTCATCCGACCACGAACAACGTAGTAGTGGCAACGGCGGAGGCGGTGATAATAAACAAGATACTAGCGAAGAGCTGCAAGATAAGAAGGAGAAAGAAATCAGCCAGACCCAATTGGCTGAGGATCAGACCGGCTCAGTGGCGGAACACAACCAACAACAAGCTGACCACGATGAACAGGGCACTCAGAAGGATTCTGTCGATCAATCTGACCAAAAAAACCAGAAGGGTGCCGTCGATCAATCCGAAAAGAACGGCGATCAAATAATCACAAGCGACGATCAAATAATCACAAGCGATGGTCAAAAGAACAGTTCAGATAGCAATTCACAAGAGCAGCAAGACACAAATAACAAGGACAACAATCATAACTACGAAAGCGGGGAAGGCAATTCTCAAGATTCTCAGAGCGATAAGAAATCGGATAATGCAGATCAAAAGCAAAATGCAGTATCGTCGGATCACCAGCAACAGCACGAACACGAATTTCTAAGCAGTGGGAAGCAGGGGAATTCGTCGCAAGAATCCCAAAACTATCAGCAGCAGCAGCAGAATTCGGAATCCAACGCGATCAAAAGCGGAGAGTTGCAGTTCTCGGACGAGACGAGCCATGCAGCTGCGAACTTGCAAGTACAAAGCAAGGAATCTACCTATCAGGACTTTTCAAAAACACCAACCGATCAGAACATACAGAATCCAGAAAAAACCCGGCAGGACTCCGACTCCGATCAGAAGAACAAGAAGGACGGCGGCGCCAATGCAGCCAATCAAAGCAAGGTTCCAACAACCGAGTCCAACTCGGGCGATTCGTTACTGAGCGGGGACAACACGGTCCGGAAGGAGTCGAAGGAGTCGAAGAAGTCGTGGTCGACGCAGGCGGCGCAGTCGGAGAACGAGAAGGAGAGGCGCAAGGACGAATCGGCCGGTCAGGACGGCATCTACGGCTACACGTGGCAGCTCTGCAACGTCACGGCGGGTCCGGATTATATACCGTGTTTGGACAACGAGAAGGCTCTGAAAAAACTGCGTTCGACCAAACACTTCCAGCACCGCGAGCGGCACTGCCCCCAGGAGGGGCCCACTTGCCTGGTCCCACTTCCGGAAGGATACCAAAAGTCCATCGCGTGGCCGAAAAGCCGAGACAAGATATGGTATCATAATGTGCCGCACACGTTGCTGGCGGAGGTGAAGGGCCACCAGAACTGGGTGAAGGTTTCCGGTGAGTTTTTGACGTTTCCCGGTGGCGGGACCCAGTTCATACATGGCGCCCTCGTGTACATTGATTTCGTTCAAAACGCAGTCCCGAAGATCGCATGGGGAAAGCACACTAGAGTTATATTGGACGTTGGTTGTGGGGTTGCAAGCTTTGGCGGATATCTCTTCGAGAGAGACGTTCTAACGATGTCGTTTGCTCCCAAAGATGAACACGAAGCACAAGTCCAGTTTGCCCTTGAGAGAGGCATACCTGCAATCTCTGCAGTCATGGGTTCTCAGAGGCTGCCCTTCCCCAGCAGGGTCTTCGATGTCGTGCATTGTGCGCGATGCAGAGTCCCTTGGCACATAGAAGGTGGTATGCTTCTTTTGGAATTGAATCGGGTTCTGCGGCCTGGGGGTTACTTTGTGTGGTCAGCCACTCCTGTTTACAGGAAGGAACAAGAGGATATCGAAATATGGAAGGAAATGTCGGCACTAACGGCCTCCATGTGTTGGGAGGTTGTAGCCATCAAGAAGGACAAGGTAAATTCAGTTGGGGCCGCCATCTACCGGAAACCAACCTCAAACCAGTGCTATGACCAAAGAAAGCAAAAGCAGCCTCCTATGTGCAAAAACGACGACGACCCTAATGCTGCCTGGTACGTACCTCTGCAGGCATGCATGCACCGGGCACCCACAGACAAATTGGAGAGAGGGACTAAATGGCCTGAGAAGTGGCCTAGTCGCCTGCAGACTCCACCTTACTGGTTAAACAGCTCCCAGATGGGCATCTATGGGAAGCCAGCACCCCAAGATTTTTCAACAGATTATGAGCACTGGAAACGTGTTATCAGTAACACTTACATGAAGAGTTTGGGTATCAACTGGTCGAACGTCAGAAATTGTATGGACATGAGAGCTGTTTATGGAGGGTTTGCTGCAGCTCTGAAGGACCTTAAAGTGTGGGTCATGAATGTGGTGAATATAGATTCACCGGATACACTCCCCATAATCTACGAACGGGGTCTTTTCGGAATTTACCATGATTGGTGTGAATCCTTTAGCACTTACCCCAGAAGCTATGACCTACTTCATGCAGATCATCTCTTTTCAAAGCTTAAAAAGAGATGCAAACTCGCTCCGGTACTGGCAGAGGTTGATCGGATTGTCAGACCAGGAGGTAAGTTTATAGTCCGGGATGAGTCGAGCACAATTGGGGAGGTTGAGAACTTGTTGAAGTCCCTGCATTGGGAGGTTAGTCTAACCGTCTCCAAAAACCAAGAAGGAATGCTCAGTGCACAGAAAGGCAACTGGCGGCCAACCACATATGCAGATAGAGATTCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

969

Amino Acids

108.18

Weight (kDa)

5.83

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 449 - 953 2.4e-232 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11 PF08241 568 - 662 2.3e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011678)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51070 AT3G51070
fragaria_vesca FvH4_1g12411
malus_domestica MD02G1136800.v1.1 MD15G1250600.v1.1
prunus_persica Prupe.7G163700_v2.0.a1
pyrus_communis pycom15g22090
rosa_chinensis RchiOBHm_Chr2g0100281
rosa_laevigata RLG00000016952
rosa_multiflora Rmu_sc0002547.1_g000021 Rmu_sc0002547.1_g000022
rosa_roxburghii Rroxscaffold_2G00142420
rosa_rugosa Rorug02G0087100 Rorug02G0087200
rosa_samantha Rh2AG136100 Rh2BG139700 Rh2CG141400 Rh2DG141600
rosa_wichuraiana Rw2G010650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1193
Acc36I ACCTGC 1 cut(s) 1837
AccB1I GGYRCC 5 cut(s) 4, 593, 1115, 1628, 2248
AccB7I CCANNNNNTGG 2 cut(s) 1488, 2070
AccBSI CCGCTC 2 cut(s) 1182, 1420
AccI GTMKAC 1 cut(s) 1232
AccII CGCG 5 cut(s) 22, 932, 1416, 1487, 1903
AccIII TCCGGA 3 cut(s) 1197, 1342, 1462
AclWI GGATC 2 cut(s) 495, 831
AcoI YGGCCR 3 cut(s) 1284, 1490, 2873
AcsI RAATTY 6 cut(s) 49, 848, 874, 916, 2104, 2592
AcuI CTGAAG 1 cut(s) 2525
AcvI CACGTG 1 cut(s) 1314
AcyI GRCGYC 3 cut(s) 5, 1116, 1629
AfaI GTAC 6 cut(s) 361, 996, 1640, 2219, 2223, 2703
AflIII ACRYGT 3 cut(s) 1311, 1533, 2401
AgsI TTSAA 8 cut(s) 278, 339, 1029, 1655, 1954, 2379, 2670, 2794
AjnI CCWGG 6 cut(s) 7, 1431, 1449, 1969, 2213, 2731
Alw21I GWGCWC 4 cut(s) 160, 2394, 2768, 2857
Alw26I GTCTC 4 cut(s) 959, 1497, 1746, 2830
Alw44I GTGCAC 1 cut(s) 2853
AlwI GGATC 2 cut(s) 495, 831
AlwNI CAGNNNCTG 1 cut(s) 1861
Ama87I CYCGRG 2 cut(s) 286, 1162
Aor13HI TCCGGA 3 cut(s) 1197, 1342, 1462
ApaI GGGCCC 1 cut(s) 1443
ApaLI GTGCAC 1 cut(s) 2853
ApoI RAATTY 6 cut(s) 49, 848, 874, 916, 2104, 2592
ArsI GACNNNNNNTTYG 2 cut(s) 1785, 1817
AspLEI GCGC 7 cut(s) 7, 24, 1118, 1246, 1272, 1631, 1903
AsuC2I CCSGG 6 cut(s) 287, 288, 1072, 1602, 2246, 2753
AsuHPI GGTGA 8 cut(s) 202, 422, 818, 1561, 1582, 1595, 2541, 2548
AvaI CYCGRG 2 cut(s) 286, 1162
AvaII GGWCC 6 cut(s) 199, 1195, 1340, 1453, 1611, 2509
BaeGI GKGCMC 4 cut(s) 548, 1443, 2251, 2857
BanI GGYRCC 5 cut(s) 4, 593, 1115, 1628, 2248
BanII GRGCYC 1 cut(s) 1443
BarI GAAGNNNNNNTAC 2 cut(s) 349, 381
BauI CACGAG 1 cut(s) 1634
BbrPI CACGTG 1 cut(s) 1314
BbsI GAAGAC 1 cut(s) 1873
Bbv12I GWGCWC 4 cut(s) 160, 2394, 2768, 2857
BbvCI CCTCAGC 1 cut(s) 483
BccI CCATC 6 cut(s) 317, 656, 1490, 2093, 2129, 2335
BceAI ACGGC 8 cut(s) 415, 581, 634, 1126, 1312, 1321, 1350, 2073
BcgI CGANNNNNNTGC 6 cut(s) 801, 835, 1864, 1898, 2189, 2223
BciT130I CCWGG 6 cut(s) 9, 1433, 1451, 1971, 2215, 2733
BciVI GTATCC 2 cut(s) 1463, 2548
BclI TGATCA 1 cut(s) 207
BcnI CCSGG 6 cut(s) 287, 288, 1072, 1602, 2246, 2753
BcoDI GTCTC 4 cut(s) 959, 1497, 1746, 2830
BfaI CTAG 4 cut(s) 203, 429, 1692, 2298
BfmI CTRYAG 4 cut(s) 1839, 2228, 2306, 2496
BfoI RGCGCY 3 cut(s) 8, 1119, 1632
BfuAI ACCTGC 1 cut(s) 1837
BfuI GTATCC 2 cut(s) 1463, 2548
BlpI GCTNAGC 1 cut(s) 329
BmcAI AGTACT 1 cut(s) 361
Bme18I GGWCC 6 cut(s) 199, 1195, 1340, 1453, 1611, 2509
BmeT110I CYCGRG 2 cut(s) 286, 1162
BmrI ACTGGG 2 cut(s) 1576, 1609
BmsI GCATC 7 cut(s) 102, 178, 295, 1308, 1895, 2355, 2675
BmuI ACTGGG 2 cut(s) 1576, 1609
BpiI GAAGAC 1 cut(s) 1873
BplI GAGNNNNNCTC 2 cut(s) 1145, 1177
Bpu10I CCTNAGC 1 cut(s) 483
Bpu1102I GCTNAGC 1 cut(s) 329
BpuEI CTTGAG 2 cut(s) 744, 1835
BpuMI CCSGG 6 cut(s) 287, 288, 1072, 1602, 2246, 2753
BsaAI YACGTR 2 cut(s) 1314, 2221
BsaBI GATNNNNATC 1 cut(s) 2345
BsaHI GRCGYC 3 cut(s) 5, 1116, 1629
BsaJI CCNNGG 5 cut(s) 195, 286, 1431, 1916, 1970
BsaWI WCCGGW 7 cut(s) 1197, 1342, 1462, 1580, 2127, 2551, 2698
BsaXI ACNNNNNCTCC 2 cut(s) 1070, 1100
Bse118I RCCGGY 2 cut(s) 494, 1286
Bse1I ACTGG 9 cut(s) 1571, 1615, 1804, 2146, 2327, 2399, 2447, 2709, 2873
Bse8I GATNNNNATC 1 cut(s) 2345
BseAI TCCGGA 3 cut(s) 1197, 1342, 1462
BseBI CCWGG 6 cut(s) 9, 1433, 1451, 1971, 2215, 2733
BseDI CCNNGG 5 cut(s) 195, 286, 1431, 1916, 1970
BseGI GGATG 3 cut(s) 117, 368, 2761
BseJI GATNNNNATC 1 cut(s) 2345
BseNI ACTGG 9 cut(s) 1571, 1615, 1804, 2146, 2327, 2399, 2447, 2709, 2873
BseRI GAGGAG 2 cut(s) 166, 169
BseSI GKGCMC 4 cut(s) 548, 1443, 2251, 2857
BseX3I CGGCCG 1 cut(s) 1284
BseYI CCCAGC 1 cut(s) 1871
Bsh1236I CGCG 5 cut(s) 22, 932, 1416, 1487, 1903
Bsh1285I CGRYCG 1 cut(s) 1287
BshNI GGYRCC 5 cut(s) 4, 593, 1115, 1628, 2248
BsiEI CGRYCG 1 cut(s) 1287
BsiHKAI GWGCWC 4 cut(s) 160, 2394, 2768, 2857
BsiHKCI CYCGRG 2 cut(s) 286, 1162
BslFI GGGAC 8 cut(s) 122, 1199, 1439, 1624, 1649, 1898, 2287, 2782
BsmAI GTCTC 4 cut(s) 959, 1497, 1746, 2830
BsmBI CGTCTC 3 cut(s) 959, 1746, 2830
BsmFI GGGAC 8 cut(s) 122, 1199, 1439, 1624, 1649, 1898, 2287, 2782
BsmI GAATGC 1 cut(s) 2850
BsoBI CYCGRG 2 cut(s) 286, 1162
Bsp120I GGGCCC 1 cut(s) 1439
Bsp1286I GDGCHC 7 cut(s) 160, 548, 1443, 2251, 2394, 2768, 2857
Bsp13I TCCGGA 3 cut(s) 1197, 1342, 1462
Bsp1407I TGTACA 1 cut(s) 1638
Bsp1720I GCTNAGC 1 cut(s) 329
BspEI TCCGGA 3 cut(s) 1197, 1342, 1462
BspFNI CGCG 5 cut(s) 22, 932, 1416, 1487, 1903
BspHI TCATGA 1 cut(s) 2526
BspMAI CTGCAG 4 cut(s) 1843, 2232, 2310, 2500
BspMI ACCTGC 1 cut(s) 1837
BspPI GGATC 2 cut(s) 495, 831
BspQI GCTCTTC 1 cut(s) 429
BspT107I GGYRCC 5 cut(s) 4, 593, 1115, 1628, 2248
BsrBI CCGCTC 2 cut(s) 1182, 1420
BsrFI RCCGGY 2 cut(s) 494, 1286
BsrGI TGTACA 1 cut(s) 1638
BsrI ACTGG 9 cut(s) 1571, 1615, 1804, 2146, 2327, 2399, 2447, 2709, 2873
BssAI RCCGGY 2 cut(s) 494, 1286
BssECI CCNNGG 5 cut(s) 195, 286, 1431, 1916, 1970
BssNI GRCGYC 3 cut(s) 5, 1116, 1629
BssSI CACGAG 1 cut(s) 1634
BssT1I CCWWGG 1 cut(s) 1916
Bst2BI CACGAG 1 cut(s) 1634
Bst2UI CCWGG 6 cut(s) 9, 1433, 1451, 1971, 2215, 2733
Bst4CI ACNGT 5 cut(s) 67, 677, 1195, 1356, 2824
Bst6I CTCTTC 3 cut(s) 429, 1748, 2420
BstACI GRCGYC 3 cut(s) 5, 1116, 1629
BstAPI GCANNNNNTGC 2 cut(s) 1537, 1838
BstAUI TGTACA 1 cut(s) 1638
BstBAI YACGTR 2 cut(s) 1314, 2221
BstC8I GCNNGC 9 cut(s) 1239, 1418, 1542, 1726, 2232, 2236, 2240, 2306, 2361
BstF5I GGATG 3 cut(s) 117, 368, 2761
BstFNI CGCG 5 cut(s) 22, 932, 1416, 1487, 1903
BstH2I RGCGCY 3 cut(s) 8, 1119, 1632
BstHHI GCGC 7 cut(s) 7, 24, 1118, 1246, 1272, 1631, 1903
BstMAI GTCTC 4 cut(s) 959, 1497, 1746, 2830
BstMCI CGRYCG 1 cut(s) 1287
BstNI CCWGG 6 cut(s) 9, 1433, 1451, 1971, 2215, 2733
BstNSI RCATGY 2 cut(s) 2238, 2242
BstSFI CTRYAG 4 cut(s) 1839, 2228, 2306, 2496
BstSLI GKGCMC 4 cut(s) 548, 1443, 2251, 2857
BstSNI TACGTA 1 cut(s) 2221
BstUI CGCG 5 cut(s) 22, 932, 1416, 1487, 1903
BstV2I GAAGAC 1 cut(s) 1873
BstXI CCANNNNNNTGG 2 cut(s) 1450, 2322
BstZI CGGCCG 1 cut(s) 1284
BsuI GTATCC 2 cut(s) 1463, 2548
BtgZI GCGATG 3 cut(s) 675, 1468, 1918
BtsCI GGATG 3 cut(s) 117, 368, 2761
BtsI GCAGTG 3 cut(s) 96, 865, 1423
BtsIMutI CAGTG 7 cut(s) 96, 507, 865, 1423, 2153, 2392, 2857
BveI ACCTGC 1 cut(s) 1837
Cac8I GCNNGC 9 cut(s) 1239, 1418, 1542, 1726, 2232, 2236, 2240, 2306, 2361
CaiI CAGNNNCTG 1 cut(s) 1861
CciI TCATGA 1 cut(s) 2526
CfoI GCGC 7 cut(s) 7, 24, 1118, 1246, 1272, 1631, 1903
Cfr10I RCCGGY 2 cut(s) 494, 1286
Cfr9I CCCGGG 1 cut(s) 286
CpoI CGGWCCG 1 cut(s) 1195
CseI GACGC 1 cut(s) 1243
Csp6I GTAC 6 cut(s) 360, 995, 1639, 2218, 2222, 2702
CspI CGGWCCG 1 cut(s) 1195
CviQI GTAC 6 cut(s) 360, 995, 1639, 2218, 2222, 2702
DinI GGCGCC 3 cut(s) 6, 1117, 1630
DrdI GACNNNNNNGTC 1 cut(s) 1193
DseDI GACNNNNNNGTC 1 cut(s) 1193
EaeI YGGCCR 3 cut(s) 1284, 1490, 2873
EagI CGGCCG 1 cut(s) 1284
Eam1104I CTCTTC 3 cut(s) 429, 1748, 2420
EarI CTCTTC 3 cut(s) 429, 1748, 2420
EciI GGCGGA 4 cut(s) 417, 521, 1559, 1749
EclXI CGGCCG 1 cut(s) 1284
Eco105I TACGTA 1 cut(s) 2221
Eco130I CCWWGG 1 cut(s) 1916
Eco24I GRGCYC 1 cut(s) 1443
Eco32I GATATC 2 cut(s) 1739, 2026
Eco47I GGWCC 6 cut(s) 199, 1195, 1340, 1453, 1611, 2509
Eco52I CGGCCG 1 cut(s) 1284
Eco57I CTGAAG 1 cut(s) 2525
Eco72I CACGTG 1 cut(s) 1314
Eco88I CYCGRG 2 cut(s) 286, 1162
EcoO109I RGGNCCY 3 cut(s) 1439, 1611, 2509
EcoRI GAATTC 3 cut(s) 49, 874, 916
EcoRII CCWGG 6 cut(s) 7, 1431, 1449, 1969, 2213, 2731
EcoRV GATATC 2 cut(s) 1739, 2026
EcoT14I CCWWGG 1 cut(s) 1916
EcoT22I ATGCAT 1 cut(s) 2240
EcoT38I GRGCYC 1 cut(s) 1443
EgeI GGCGCC 3 cut(s) 6, 1117, 1630
EheI GGCGCC 3 cut(s) 6, 1117, 1630
ErhI CCWWGG 1 cut(s) 1916
Esp3I CGTCTC 3 cut(s) 959, 1746, 2830
FaqI GGGAC 8 cut(s) 122, 1199, 1439, 1624, 1649, 1898, 2287, 2782
FauI CCCGC 4 cut(s) 737, 1175, 1330, 1601
FauNDI CATATG 1 cut(s) 2884
FbaI TGATCA 1 cut(s) 207
FblI GTMKAC 1 cut(s) 1232
FokI GGATG 3 cut(s) 124, 355, 2768
FriOI GRGCYC 1 cut(s) 1443
FspBI CTAG 4 cut(s) 203, 429, 1692, 2298
GlaI GCGC 7 cut(s) 6, 23, 1117, 1245, 1271, 1630, 1902
GsaI CCCAGC 1 cut(s) 1875
HaeII RGCGCY 3 cut(s) 8, 1119, 1632
HgaI GACGC 1 cut(s) 1243
HhaI GCGC 7 cut(s) 7, 24, 1118, 1246, 1272, 1631, 1903
Hin1I GRCGYC 3 cut(s) 5, 1116, 1629
Hin6I GCGC 7 cut(s) 5, 22, 1116, 1244, 1270, 1629, 1901
HinP1I GCGC 7 cut(s) 5, 22, 1116, 1244, 1270, 1629, 1901
HincII GTYRAC 1 cut(s) 1233
HindII GTYRAC 1 cut(s) 1233
HindIII AAGCTT 2 cut(s) 1726, 2672
HphI GGTGA 8 cut(s) 202, 422, 818, 1561, 1582, 1595, 2541, 2548
Hpy166II GTNNAC 4 cut(s) 1233, 1639, 2005, 2855
Hpy8I GTNNAC 4 cut(s) 1233, 1639, 2005, 2855
Hpy99I CGWCG 9 cut(s) 49, 305, 602, 644, 823, 883, 1237, 2198, 2201
HpyCH4III ACNGT 5 cut(s) 67, 677, 1195, 1356, 2824
Hsp92I GRCGYC 3 cut(s) 5, 1116, 1629
HspAI GCGC 7 cut(s) 5, 22, 1116, 1244, 1270, 1629, 1901
KasI GGCGCC 3 cut(s) 4, 1115, 1628
KflI GGGWCCC 1 cut(s) 1611
Kpn2I TCCGGA 3 cut(s) 1197, 1342, 1462
Ksp22I TGATCA 1 cut(s) 207
LguI GCTCTTC 1 cut(s) 429
LmnI GCTCC 3 cut(s) 1780, 2339, 2701
LweI GCATC 7 cut(s) 102, 178, 295, 1308, 1895, 2355, 2675
MaeI CTAG 4 cut(s) 203, 429, 1692, 2298
MaeIII GTNAC 5 cut(s) 67, 1173, 1330, 1976, 2411
MbiI CCGCTC 2 cut(s) 1182, 1420
MfeI CAATTG 2 cut(s) 476, 2769
MhlI GDGCHC 7 cut(s) 160, 548, 1443, 2251, 2394, 2768, 2857
Mly113I GGCGCC 3 cut(s) 5, 1116, 1629
MlyI GAGTC 9 cut(s) 144, 1073, 1079, 1163, 1214, 1223, 1920, 2305, 2768
MmeI TCCRAC 7 cut(s) 396, 801, 951, 1107, 1169, 1182, 1230
Mph1103I ATGCAT 1 cut(s) 2240
MroI TCCGGA 3 cut(s) 1197, 1342, 1462
MseI TTAA 5 cut(s) 14, 2327, 2514, 2676, 2905
MslI CAYNNNNRTG 3 cut(s) 89, 2420, 2531
MspA1I CMGCKG 1 cut(s) 980
MunI CAATTG 2 cut(s) 476, 2769
Mva1269I GAATGC 1 cut(s) 2850
MvaI CCWGG 6 cut(s) 9, 1433, 1451, 1971, 2215, 2733
MvnI CGCG 5 cut(s) 22, 932, 1416, 1487, 1903
NarI GGCGCC 3 cut(s) 5, 1116, 1629
NciI CCSGG 6 cut(s) 287, 288, 1072, 1602, 2246, 2753
NdeI CATATG 1 cut(s) 2884
NmeAIII GCCGAG 1 cut(s) 1526
NmuCI GTSAC 2 cut(s) 67, 1330
NsiI ATGCAT 1 cut(s) 2240
NspI RCATGY 2 cut(s) 2238, 2242
PaeI GCATGC 2 cut(s) 2238, 2242
PagI TCATGA 1 cut(s) 2526
PciSI GCTCTTC 1 cut(s) 429
PcsI WCGNNNNNNNCGW 7 cut(s) 68, 606, 965, 1169, 1214, 1223, 1752
PctI GAATGC 1 cut(s) 2850
PflMI CCANNNNNTGG 2 cut(s) 1488, 2070
PfoI TCCNGGA 1 cut(s) 2751
PleI GAGTC 9 cut(s) 143, 1073, 1079, 1162, 1213, 1222, 1919, 2305, 2767
PluTI GGCGCC 3 cut(s) 8, 1119, 1632
PmaCI CACGTG 1 cut(s) 1314
PmlI CACGTG 1 cut(s) 1314
PpsI GAGTC 9 cut(s) 143, 1073, 1079, 1162, 1213, 1222, 1919, 2305, 2767
Ppu21I YACGTR 2 cut(s) 1314, 2221
PpuMI RGGWCCY 2 cut(s) 1611, 2509
Psp5II RGGWCCY 2 cut(s) 1611, 2509
Psp6I CCWGG 6 cut(s) 7, 1431, 1449, 1969, 2213, 2731
PspCI CACGTG 1 cut(s) 1314
PspFI CCCAGC 1 cut(s) 1871
PspGI CCWGG 6 cut(s) 7, 1431, 1449, 1969, 2213, 2731
PspOMI GGGCCC 1 cut(s) 1439
PspPPI RGGWCCY 2 cut(s) 1611, 2509
PstI CTGCAG 4 cut(s) 1843, 2232, 2310, 2500
PstNI CAGNNNCTG 1 cut(s) 1861
PvuII CAGCTG 1 cut(s) 980
RsaI GTAC 6 cut(s) 361, 996, 1640, 2219, 2223, 2703
RsaNI GTAC 6 cut(s) 360, 995, 1639, 2218, 2222, 2702
RseI CAYNNNNRTG 3 cut(s) 89, 2420, 2531
Rsr2I CGGWCCG 1 cut(s) 1195
RsrII CGGWCCG 1 cut(s) 1195
SalI GTCGAC 1 cut(s) 1231
SapI GCTCTTC 1 cut(s) 429
SaqAI TTAA 5 cut(s) 14, 2327, 2514, 2676, 2905
ScaI AGTACT 1 cut(s) 361
SchI GAGTC 9 cut(s) 144, 1073, 1079, 1163, 1214, 1223, 1920, 2305, 2768
SduI GDGCHC 7 cut(s) 160, 548, 1443, 2251, 2394, 2768, 2857
SfaNI GCATC 7 cut(s) 102, 178, 295, 1308, 1895, 2355, 2675
SfcI CTRYAG 4 cut(s) 1839, 2228, 2306, 2496
SfoI GGCGCC 3 cut(s) 6, 1117, 1630
SinI GGWCC 6 cut(s) 199, 1195, 1340, 1453, 1611, 2509
SmaI CCCGGG 1 cut(s) 288
SmiMI CAYNNNNRTG 3 cut(s) 89, 2420, 2531
SmlI CTYRAG 2 cut(s) 759, 1814
SmoI CTYRAG 2 cut(s) 759, 1814
SnaBI TACGTA 1 cut(s) 2221
SphI GCATGC 2 cut(s) 2238, 2242
SspDI GGCGCC 3 cut(s) 4, 1115, 1628
SspMI CTAG 4 cut(s) 203, 429, 1692, 2298
StyI CCWWGG 1 cut(s) 1916
TaaI ACNGT 5 cut(s) 67, 677, 1195, 1356, 2824
TatI WGTACW 3 cut(s) 359, 994, 1638
TauI GCSGC 7 cut(s) 1116, 1244, 1423, 1531, 1969, 2120, 2875
Tru1I TTAA 5 cut(s) 14, 2327, 2514, 2676, 2905
Tru9I TTAA 5 cut(s) 14, 2327, 2514, 2676, 2905
TscAI CASTG 7 cut(s) 96, 507, 865, 1430, 2153, 2399, 2857
TseFI GTSAC 2 cut(s) 67, 1330
Tsp45I GTSAC 2 cut(s) 67, 1330
TspDTI ATGAA 9 cut(s) 73, 273, 357, 552, 1609, 1800, 2438, 2543, 2639
TspMI CCCGGG 1 cut(s) 286
TspRI CASTG 7 cut(s) 96, 507, 865, 1430, 2153, 2399, 2857
Van91I CCANNNNNTGG 2 cut(s) 1488, 2070
VneI GTGCAC 1 cut(s) 2853
VpaK11BI GGWCC 6 cut(s) 199, 1195, 1340, 1453, 1611, 2509
XapI RAATTY 6 cut(s) 49, 848, 874, 916, 2104, 2592
XceI RCATGY 2 cut(s) 2238, 2242
XcmI CCANNNNNNNNNTGG 2 cut(s) 1622, 2260
XmaI CCCGGG 1 cut(s) 286
XmiI GTMKAC 1 cut(s) 1232
XspI CTAG 4 cut(s) 203, 429, 1692, 2298
ZrmI AGTACT 1 cut(s) 361
Zsp2I ATGCAT 1 cut(s) 2240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.