MD15G1250600.v1.1

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011678
Physical Location & Seq
Reverse (-)
20996101 .. 21000205
4105 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1250600.v1.1.491

Sequence Viewer

Length: 2898 bp
ATGGCGCTAGGGTTAAGGTCGCGCAGCAACAAGCGATCTTCGTCGTCGAATTCTTACGCTTCTACTGTGACGATTATGGTGTTCATATCAGTGTGCGTTTTGGCCGTGTGGATGCTCAACTACTCTAACTCAGGAGTCCCGCAAACCTCCACAAGTGCTCGAACTGCCACGACCTCCTCCACAGACGATGCTTCGTCGGATGATACTCAGGCCCAAGTGATCACCAAAAACACAAACAATGACGACGACAACAACAATAACAAGAACAATATTATTAACGAAACACCGAAGGCTTTTGAAGACAACCCGGGTGATCTCCCCGACGATGCCATCAAATCCGATGATCTGCTGAACAATTCAACAAAGGGTAGTGGTAGTAGTACTAGTTCATCAGAGCAAGACCAAAGTAGTACTGGCGATGGCGGAGGCCGCGGCGGTGATAATAAACAAGATTCTAGCGAAGTGCAGAAAGATCAGAAGGAGAAAGAAATCAGCCAGACCCAATTGGCTGAGGATCAGAGCGGCTCAGTGGTGGAACACAACCAACAACAAGCAGACCACGATGAACAGGGCACTCAGAAGGATTCTGACGATCAATCTGATCATAAAAAACAGAAGGGTGCCGACGATCAATCCGAAAAGAACGGCGATCAAATTATCACAAGCGACGATCAAACGAACAATTCAAAGAGCAATTCACAAGAGCATCAAGACACAAACAACAATGACAAGAATAATAATTCCGAAAGCAATAAGAAATCGGATAATGCAGATCAGCAGCAAAATGCAGCATCGTCGGATCAACAGCAACAGCAAGAACAAGAATTTCTAAGCAATGGGAAGCAGGGGAATTCATCGCAAGAATCCCAAAACGATCAGCAGCAGCAGCAACAGAATTGGGAATACAACGCGACCAAAAGCGGACAGTTGCAGTTCTCGGACGAGACGAGCAATGCAGTTGCCAGCTTGCAAGTACAGAGCAAGGAATCTACCGATCAGAACTTTTCACAAACACCTTCCGATCAGAACAAAAAGAACCTAGAAAAAACCCAGCAGCACTCCGACTCCGATCAGAAGAAGAAGGACGGCGGCAGCAGCGGCGGCGGAGGCAATGCAACCAATCAAAGCAAGGGTCCGGCAACTGAGTCCAACTCGGGCGATTCGCTATTGAGCGGGGACAACACTGTTCGGAAGGAGTCGAAGGAGTCAAAGAAGTCGTGGTCGACGCAGGCGGCGCAGTCGGAGAGCGAGAAGGAAAGGCGCAAGGACGAATCGGACGGTCAGGACGGCATCTACGGATACACGTGGCAGCTCTGCAACGTCACGGCGGGTCCGGATTATATACCGTGTTTGGACAACGAGAAGGCTCTGAAAAAACTGCGTTCGACCAAACACTTCGAGCACCGGGAGCGGCACTGCCGGCAGGAGGGGCCCACGTGCCTGGTCCCACTACCGGACGGATACAAAATGTCCATCGAGTGGCCGAAAAGCAGAGACAAGATATGGTATCATAATGTGCCGCACACGTTGCTGGCGGAGGTGAAGGGCCACCAGAACTGGGTGAAGGTTTCCGGTGAGTTTTTGACGTTTCCCGGCGGCGGGACGCAGTTCATACATGGCGCCCTCGTGTACATCGATTTCCTTCAAAACGCAGTCCCGAACATCGCATGGGGTGAGCACACTAGAGTTATATTGGACGTTGGTTGTGGGGTTGCAAGCTTTGGTGGATATCTCTTCGACAGAGATGTTCTAACGATGTCGTTTGCACCGAAAGATGAACACGAAGCTCAAGTCCAGTTTGCCCTTGAGAGAGGCATACCCGCAATCTCTGCAGTCATGGGTTCTCAGAGGCTGCCCTTCCCCAGCAGGGTCTTCGATGTCGTGCATTGTGCGAGATGCAGAGTCCCTTGGCACAGAGAAGGTGGTACGCTTCTTTTGGAATTGAACCGGGTTCTGCGGCCCGGGGGTTACTTTGTATGGTCAGCCACTCCTGTTTACAGGAAGGAACAAGAAGATATCGAAATTTGGAAGGAGATGTCTGCACTGACGGCCTCCATGTGTTGGGAGGTTGTAGCCATCAAGAACGACAAGGTTAATTTAGTTGCGGCCGCCGTCTACCGTAAACCAACCTCAAATCAATGCTACGACCAAAGAAAGCAAAAGCAGCCTCCTATGTGCAAAAACGACGATGACCCGAATGCTGCCTGGTACGTACCTCTACAGGGATGCATGCACCGGGTACCCACAGACAAATCGGAGAGAGGGACTAAATGGCCTGAGAAGTGGCCTAGTCGCCTGCAGACTCCGCCTTACTGGTTAAACAGCTCCCAAATGGGCATCTATGGGAAGCCAGCACCCCAAGATTTTTCAACAGATTACGAGCACTGGAAACGTGTTATCAGTAACACTTACATGAAGAGTTTGGGTATCAACTGGTCGAACGTCAGAAATTGTATGGATATGAGAGCTGTTTATGGAGGATTTGCTGCAGCTCTGAAGGACCTTAAAGTGTGGGTCATGAATGTGGTGAACGTAGATTCACCAGATACACTCCCCATAATCTACGAACGGGGTCTTTTCGGAATTTACCATGACTGGTGTGAATCGTTTAGCACTTACCCCAGAACCTATGACCTACTTCATGCAGATCATCTCTTTTCAAAGCTTAAAAAGAGATGCAAACTTGCTCCGGTACTGGCAGAGGTTGATCGGATTGTCAGACCAGGAGGTAAGTTTATAGTCCGGGATGAGTCGAGCACAATTGGGGAGGTTGAGAACTTGTTGAAGTCCCTGCATTGGGAAGTTAGTCTAACCGTCTCCAAAAACCAAGAAGGAATGCTCAGTGCGCAGAAAGGGAACTGGCGGCCAACCACATATGCAGATAGAGATTCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

966

Amino Acids

107.8

Weight (kDa)

5.53

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 446 - 950 1.3e-232 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_25 PF13649 564 - 656 7.1e-06 Methyltransferase domain
Methyltransf_11 PF08241 565 - 659 1.3e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011678)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51070 AT3G51070
fragaria_vesca FvH4_1g12411
malus_domestica MD02G1136800.v1.1 MD15G1250600.v1.1
prunus_persica Prupe.7G163700_v2.0.a1
pyrus_communis pycom15g22090
rosa_chinensis RchiOBHm_Chr2g0100281
rosa_laevigata RLG00000016952
rosa_multiflora Rmu_sc0002547.1_g000021 Rmu_sc0002547.1_g000022
rosa_roxburghii Rroxscaffold_2G00142420
rosa_rugosa Rorug02G0087100 Rorug02G0087200
rosa_samantha Rh2AG136100 Rh2BG139700 Rh2CG141400 Rh2DG141600
rosa_wichuraiana Rw2G010650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2847
Acc65I GGTACC 1 cut(s) 2239
AccB1I GGYRCC 3 cut(s) 620, 1619, 2239
AccB7I CCANNNNNTGG 2 cut(s) 1479, 2061
AccBSI CCGCTC 3 cut(s) 522, 1173, 1411
AccI GTMKAC 2 cut(s) 1223, 2115
AccII CGCG 3 cut(s) 22, 432, 911
AccIII TCCGGA 1 cut(s) 1333
AclWI GGATC 2 cut(s) 522, 807
AcoI YGGCCR 4 cut(s) 102, 1481, 2106, 2864
AcsI RAATTY 5 cut(s) 49, 824, 850, 2022, 2583
AcuI CTGAAG 1 cut(s) 2516
AcvI CACGTG 2 cut(s) 1305, 1437
AcyI GRCGYC 1 cut(s) 1620
AfaI GTAC 9 cut(s) 382, 412, 975, 1631, 1927, 2210, 2214, 2241, 2694
AflIII ACRYGT 3 cut(s) 1302, 1524, 2392
AgsI TTSAA 8 cut(s) 299, 360, 687, 1646, 1945, 2370, 2661, 2785
AhlI ACTAGT 1 cut(s) 383
AjnI CCWGG 3 cut(s) 1440, 2204, 2722
AluBI AGCT 8 cut(s) 966, 1312, 1719, 1787, 2325, 2468, 2492, 2665
AluI AGCT 8 cut(s) 966, 1312, 1719, 1787, 2325, 2468, 2492, 2665
Alw21I GWGCWC 5 cut(s) 160, 1404, 1680, 2385, 2759
Alw26I GTCTC 3 cut(s) 938, 1488, 2821
AlwI GGATC 2 cut(s) 522, 807
AlwNI CAGNNNCTG 1 cut(s) 1852
Ama87I CYCGRG 3 cut(s) 307, 1153, 1961
Aor13HI TCCGGA 1 cut(s) 1333
ApaI GGGCCC 1 cut(s) 1434
ApoI RAATTY 5 cut(s) 49, 824, 850, 2022, 2583
ArsI GACNNNNNNTTYG 4 cut(s) 1117, 1149, 1776, 1808
Asp718I GGTACC 1 cut(s) 2239
AspLEI GCGC 6 cut(s) 7, 24, 1237, 1263, 1622, 2848
AspS9I GGNCC 9 cut(s) 211, 1133, 1331, 1430, 1431, 1444, 1546, 1959, 2500
AsuC2I CCSGG 9 cut(s) 308, 309, 1406, 1593, 1949, 1962, 1963, 2237, 2744
AsuHPI GGTGA 9 cut(s) 214, 323, 449, 1552, 1573, 1586, 1685, 2532, 2539
AvaI CYCGRG 3 cut(s) 307, 1153, 1961
AvaII GGWCC 4 cut(s) 1133, 1331, 1444, 2500
BaeGI GKGCMC 2 cut(s) 575, 1434
BanI GGYRCC 3 cut(s) 620, 1619, 2239
BanII GRGCYC 1 cut(s) 1434
BauI CACGAG 1 cut(s) 1625
BbrPI CACGTG 2 cut(s) 1305, 1437
BbsI GAAGAC 2 cut(s) 306, 1864
Bbv12I GWGCWC 5 cut(s) 160, 1404, 1680, 2385, 2759
BbvCI CCTCAGC 1 cut(s) 510
BccI CCATC 4 cut(s) 338, 413, 1481, 2084
BceAI ACGGC 7 cut(s) 89, 661, 1102, 1303, 1341, 2064, 2096
BcgI CGANNNNNNTGC 4 cut(s) 777, 811, 1855, 1889
BciT130I CCWGG 3 cut(s) 1442, 2206, 2724
BciVI GTATCC 2 cut(s) 1292, 1454
BclI TGATCA 2 cut(s) 219, 601
BcnI CCSGG 9 cut(s) 308, 309, 1406, 1593, 1949, 1962, 1963, 2237, 2744
BcoDI GTCTC 3 cut(s) 938, 1488, 2821
BcuI ACTAGT 1 cut(s) 383
BfaI CTAG 6 cut(s) 8, 384, 456, 1040, 1683, 2289
BfmI CTRYAG 4 cut(s) 1830, 2219, 2297, 2487
BfoI RGCGCY 2 cut(s) 8, 1623
BfuI GTATCC 2 cut(s) 1292, 1454
BmcAI AGTACT 2 cut(s) 382, 412
Bme18I GGWCC 4 cut(s) 1133, 1331, 1444, 2500
BmeT110I CYCGRG 3 cut(s) 307, 1153, 1961
BmgT120I GGNCC 9 cut(s) 211, 1133, 1331, 1430, 1431, 1444, 1546, 1959, 2500
BmiI GGNNCC 8 cut(s) 622, 1134, 1332, 1431, 1432, 1446, 1621, 2241
BmrI ACTGGG 1 cut(s) 1567
BmuI ACTGGG 1 cut(s) 1567
BpiI GAAGAC 2 cut(s) 306, 1864
BplI GAGNNNNNCTC 2 cut(s) 1136, 1168
Bpu10I CCTNAGC 1 cut(s) 510
BpuEI CTTGAG 2 cut(s) 1773, 1826
BpuMI CCSGG 9 cut(s) 308, 309, 1406, 1593, 1949, 1962, 1963, 2237, 2744
Bsa29I ATCGAT 1 cut(s) 1635
BsaAI YACGTR 3 cut(s) 1305, 1437, 2212
BsaHI GRCGYC 1 cut(s) 1620
BsaJI CCNNGG 5 cut(s) 307, 430, 1907, 1961, 1962
BsaWI WCCGGW 4 cut(s) 1333, 1453, 1571, 2689
BsaXI ACNNNNNCTCC 2 cut(s) 1049, 1079
Bse118I RCCGGY 1 cut(s) 1419
Bse1I ACTGG 9 cut(s) 418, 1562, 1795, 2318, 2390, 2438, 2600, 2700, 2864
Bse3DI GCAATG 3 cut(s) 841, 958, 1117
BseAI TCCGGA 1 cut(s) 1333
BseBI CCWGG 3 cut(s) 1442, 2206, 2724
BseCI ATCGAT 1 cut(s) 1635
BseDI CCNNGG 5 cut(s) 307, 430, 1907, 1961, 1962
BseGI GGATG 4 cut(s) 117, 205, 2231, 2752
BseMI GCAATG 3 cut(s) 841, 958, 1117
BseMII CTCAG 9 cut(s) 144, 221, 501, 540, 590, 1134, 1859, 2268, 2854
BseNI ACTGG 9 cut(s) 418, 1562, 1795, 2318, 2390, 2438, 2600, 2700, 2864
BseRI GAGGAG 1 cut(s) 166
BseSI GKGCMC 2 cut(s) 575, 1434
BseX3I CGGCCG 1 cut(s) 2106
BseYI CCCAGC 2 cut(s) 1050, 1862
BsgI GTGCAG 2 cut(s) 485, 2025
Bsh1236I CGCG 3 cut(s) 22, 432, 911
Bsh1285I CGRYCG 1 cut(s) 2109
BshNI GGYRCC 3 cut(s) 620, 1619, 2239
BshVI ATCGAT 1 cut(s) 1635
BsiEI CGRYCG 1 cut(s) 2109
BsiHKAI GWGCWC 5 cut(s) 160, 1404, 1680, 2385, 2759
BsiHKCI CYCGRG 3 cut(s) 307, 1153, 1961
BslFI GGGAC 8 cut(s) 122, 1190, 1430, 1615, 1640, 1889, 2278, 2773
BsmAI GTCTC 3 cut(s) 938, 1488, 2821
BsmBI CGTCTC 2 cut(s) 938, 2821
BsmFI GGGAC 8 cut(s) 122, 1190, 1430, 1615, 1640, 1889, 2278, 2773
BsmI GAATGC 2 cut(s) 2203, 2841
BsoBI CYCGRG 3 cut(s) 307, 1153, 1961
Bsp120I GGGCCC 1 cut(s) 1430
Bsp1286I GDGCHC 7 cut(s) 160, 575, 1404, 1434, 1680, 2385, 2759
Bsp13I TCCGGA 1 cut(s) 1333
Bsp1407I TGTACA 1 cut(s) 1629
BspCNI CTCAG 9 cut(s) 143, 220, 502, 539, 589, 1135, 1858, 2269, 2853
BspDI ATCGAT 1 cut(s) 1635
BspEI TCCGGA 1 cut(s) 1333
BspFNI CGCG 3 cut(s) 22, 432, 911
BspHI TCATGA 1 cut(s) 2517
BspLI GGNNCC 8 cut(s) 622, 1134, 1332, 1431, 1432, 1446, 1621, 2241
BspMAI CTGCAG 3 cut(s) 1834, 2301, 2491
BspPI GGATC 2 cut(s) 522, 807
BspT107I GGYRCC 3 cut(s) 620, 1619, 2239
BsrBI CCGCTC 3 cut(s) 522, 1173, 1411
BsrDI GCAATG 3 cut(s) 841, 958, 1117
BsrFI RCCGGY 1 cut(s) 1419
BsrGI TGTACA 1 cut(s) 1629
BsrI ACTGG 9 cut(s) 418, 1562, 1795, 2318, 2390, 2438, 2600, 2700, 2864
BssAI RCCGGY 1 cut(s) 1419
BssECI CCNNGG 5 cut(s) 307, 430, 1907, 1961, 1962
BssNI GRCGYC 1 cut(s) 1620
BssSI CACGAG 1 cut(s) 1625
BssT1I CCWWGG 1 cut(s) 1907
Bst2BI CACGAG 1 cut(s) 1625
Bst2UI CCWGG 3 cut(s) 1442, 2206, 2724
Bst4CI ACNGT 7 cut(s) 67, 927, 1186, 1280, 1347, 2120, 2815
Bst6I CTCTTC 2 cut(s) 1739, 2411
BstACI GRCGYC 1 cut(s) 1620
BstAPI GCANNNNNTGC 2 cut(s) 1528, 1829
BstAUI TGTACA 1 cut(s) 1629
BstBAI YACGTR 3 cut(s) 1305, 1437, 2212
BstC8I GCNNGC 9 cut(s) 964, 968, 1230, 1421, 1533, 1717, 2231, 2297, 2352
BstDSI CCRYGG 1 cut(s) 430
BstF5I GGATG 4 cut(s) 117, 205, 2231, 2752
BstFNI CGCG 3 cut(s) 22, 432, 911
BstH2I RGCGCY 2 cut(s) 8, 1623
BstHHI GCGC 6 cut(s) 7, 24, 1237, 1263, 1622, 2848
BstMAI GTCTC 3 cut(s) 938, 1488, 2821
BstMCI CGRYCG 1 cut(s) 2109
BstNI CCWGG 3 cut(s) 1442, 2206, 2724
BstNSI RCATGY 1 cut(s) 2233
BstSFI CTRYAG 4 cut(s) 1830, 2219, 2297, 2487
BstSLI GKGCMC 2 cut(s) 575, 1434
BstSNI TACGTA 1 cut(s) 2212
BstUI CGCG 3 cut(s) 22, 432, 911
BstV2I GAAGAC 2 cut(s) 306, 1864
BstXI CCANNNNNNTGG 1 cut(s) 1441
BstZI CGGCCG 1 cut(s) 2106
Bsu15I ATCGAT 1 cut(s) 1635
BsuI GTATCC 2 cut(s) 1292, 1454
BsuTUI ATCGAT 1 cut(s) 1635
BtgI CCRYGG 1 cut(s) 430
BtgZI GCGATG 3 cut(s) 432, 840, 1648
BtsCI GGATG 4 cut(s) 117, 205, 2231, 2752
BtsI GCAGTG 1 cut(s) 1414
BtsIMutI CAGTG 7 cut(s) 96, 534, 1182, 1414, 2042, 2383, 2848
Cac8I GCNNGC 9 cut(s) 964, 968, 1230, 1421, 1533, 1717, 2231, 2297, 2352
CaiI CAGNNNCTG 1 cut(s) 1852
CciI TCATGA 1 cut(s) 2517
CciNI GCGGCCGC 1 cut(s) 2106
CfoI GCGC 6 cut(s) 7, 24, 1237, 1263, 1622, 2848
Cfr10I RCCGGY 1 cut(s) 1419
Cfr13I GGNCC 9 cut(s) 211, 1133, 1331, 1430, 1431, 1444, 1546, 1959, 2500
Cfr42I CCGCGG 1 cut(s) 433
Cfr9I CCCGGG 2 cut(s) 307, 1961
ClaI ATCGAT 1 cut(s) 1635
CseI GACGC 2 cut(s) 1234, 1612
Csp6I GTAC 9 cut(s) 381, 411, 974, 1630, 1926, 2209, 2213, 2240, 2693
CviAII CATG 9 cut(s) 1616, 1668, 1837, 2056, 2230, 2413, 2518, 2591, 2642
CviQI GTAC 9 cut(s) 381, 411, 974, 1630, 1926, 2209, 2213, 2240, 2693
DinI GGCGCC 1 cut(s) 1621
EaeI YGGCCR 4 cut(s) 102, 1481, 2106, 2864
EagI CGGCCG 1 cut(s) 2106
Eam1104I CTCTTC 2 cut(s) 1739, 2411
EarI CTCTTC 2 cut(s) 1739, 2411
EciI GGCGGA 4 cut(s) 438, 1119, 1550, 2295
EclXI CGGCCG 1 cut(s) 2106
Eco105I TACGTA 1 cut(s) 2212
Eco130I CCWWGG 1 cut(s) 1907
Eco24I GRGCYC 1 cut(s) 1434
Eco32I GATATC 2 cut(s) 1730, 2017
Eco47I GGWCC 4 cut(s) 1133, 1331, 1444, 2500
Eco52I CGGCCG 1 cut(s) 2106
Eco57I CTGAAG 1 cut(s) 2516
Eco72I CACGTG 2 cut(s) 1305, 1437
Eco88I CYCGRG 3 cut(s) 307, 1153, 1961
EcoO109I RGGNCCY 2 cut(s) 1430, 2500
EcoRI GAATTC 2 cut(s) 49, 850
EcoRII CCWGG 3 cut(s) 1440, 2204, 2722
EcoRV GATATC 2 cut(s) 1730, 2017
EcoT14I CCWWGG 1 cut(s) 1907
EcoT22I ATGCAT 1 cut(s) 2231
EcoT38I GRGCYC 1 cut(s) 1434
EgeI GGCGCC 1 cut(s) 1621
EheI GGCGCC 1 cut(s) 1621
ErhI CCWWGG 1 cut(s) 1907
Esp3I CGTCTC 2 cut(s) 938, 2821
FaeI CATG 9 cut(s) 1619, 1671, 1840, 2059, 2233, 2416, 2521, 2594, 2645
FaqI GGGAC 8 cut(s) 122, 1190, 1430, 1615, 1640, 1889, 2278, 2773
FatI CATG 9 cut(s) 1615, 1667, 1836, 2055, 2229, 2412, 2517, 2590, 2641
FauI CCCGC 5 cut(s) 147, 1166, 1321, 1592, 1828
FauNDI CATATG 1 cut(s) 2875
FbaI TGATCA 2 cut(s) 219, 601
FblI GTMKAC 2 cut(s) 1223, 2115
FokI GGATG 4 cut(s) 124, 212, 2238, 2759
FriOI GRGCYC 1 cut(s) 1434
FspBI CTAG 6 cut(s) 8, 384, 456, 1040, 1683, 2289
FspI TGCGCA 1 cut(s) 2847
GlaI GCGC 6 cut(s) 6, 23, 1236, 1262, 1621, 2847
GsaI CCCAGC 2 cut(s) 1054, 1866
HaeII RGCGCY 2 cut(s) 8, 1623
HgaI GACGC 2 cut(s) 1234, 1612
HhaI GCGC 6 cut(s) 7, 24, 1237, 1263, 1622, 2848
Hin1I GRCGYC 1 cut(s) 1620
Hin1II CATG 9 cut(s) 1619, 1671, 1840, 2059, 2233, 2416, 2521, 2594, 2645
Hin6I GCGC 6 cut(s) 5, 22, 1235, 1261, 1620, 2846
HinP1I GCGC 6 cut(s) 5, 22, 1235, 1261, 1620, 2846
HincII GTYRAC 1 cut(s) 1224
HindII GTYRAC 1 cut(s) 1224
HindIII AAGCTT 2 cut(s) 1717, 2663
HphI GGTGA 9 cut(s) 214, 323, 449, 1552, 1573, 1586, 1685, 2532, 2539
Hpy166II GTNNAC 6 cut(s) 1224, 1630, 1996, 2116, 2123, 2530
Hpy188III TCNNGA 7 cut(s) 132, 710, 1283, 1334, 1657, 2080, 2518
Hpy8I GTNNAC 6 cut(s) 1224, 1630, 1996, 2116, 2123, 2530
HpyCH4III ACNGT 7 cut(s) 67, 927, 1186, 1280, 1347, 2120, 2815
Hsp92I GRCGYC 1 cut(s) 1620
Hsp92II CATG 9 cut(s) 1619, 1671, 1840, 2059, 2233, 2416, 2521, 2594, 2645
HspAI GCGC 6 cut(s) 5, 22, 1235, 1261, 1620, 2846
KasI GGCGCC 1 cut(s) 1619
Kpn2I TCCGGA 1 cut(s) 1333
KpnI GGTACC 1 cut(s) 2243
KroI GCCGGC 1 cut(s) 1419
KroNI GCCGGC 1 cut(s) 1421
Ksp22I TGATCA 2 cut(s) 219, 601
KspI CCGCGG 1 cut(s) 433
LmnI GCTCC 3 cut(s) 1408, 2330, 2692
MaeI CTAG 6 cut(s) 8, 384, 456, 1040, 1683, 2289
MaeIII GTNAC 4 cut(s) 67, 1321, 1967, 2402
MbiI CCGCTC 3 cut(s) 522, 1173, 1411
MboII GAAGA 9 cut(s) 30, 311, 1087, 1090, 1726, 1864, 2024, 2428, 2883
MfeI CAATTG 2 cut(s) 503, 2760
MhlI GDGCHC 7 cut(s) 160, 575, 1404, 1434, 1680, 2385, 2759
Mly113I GGCGCC 1 cut(s) 1620
MlyI GAGTC 8 cut(s) 144, 1058, 1154, 1205, 1214, 1911, 2296, 2759
MmeI TCCRAC 5 cut(s) 177, 777, 1086, 1173, 1221
Mph1103I ATGCAT 1 cut(s) 2231
MroI TCCGGA 1 cut(s) 1333
MroNI GCCGGC 1 cut(s) 1419
MseI TTAA 7 cut(s) 14, 276, 2094, 2318, 2505, 2667, 2896
MslI CAYNNNNRTG 3 cut(s) 89, 2411, 2522
MspA1I CMGCKG 2 cut(s) 432, 1098
MunI CAATTG 2 cut(s) 503, 2760
Mva1269I GAATGC 2 cut(s) 2203, 2841
MvaI CCWGG 3 cut(s) 1442, 2206, 2724
MvnI CGCG 3 cut(s) 22, 432, 911
NaeI GCCGGC 1 cut(s) 1421
NarI GGCGCC 1 cut(s) 1620
NciI CCSGG 9 cut(s) 308, 309, 1406, 1593, 1949, 1962, 1963, 2237, 2744
NdeI CATATG 1 cut(s) 2875
NgoMIV GCCGGC 1 cut(s) 1419
NlaIII CATG 9 cut(s) 1619, 1671, 1840, 2059, 2233, 2416, 2521, 2594, 2645
NlaIV GGNNCC 8 cut(s) 622, 1134, 1332, 1431, 1432, 1446, 1621, 2241
NmuCI GTSAC 2 cut(s) 67, 1321
NotI GCGGCCGC 1 cut(s) 2106
NsbI TGCGCA 1 cut(s) 2847
NsiI ATGCAT 1 cut(s) 2231
NspI RCATGY 1 cut(s) 2233
PaeI GCATGC 1 cut(s) 2233
PagI TCATGA 1 cut(s) 2517
PcsI WCGNNNNNNNCGW 4 cut(s) 633, 944, 1632, 1766
PctI GAATGC 2 cut(s) 2203, 2841
PdiI GCCGGC 1 cut(s) 1421
PfeI GAWTC 9 cut(s) 452, 584, 863, 986, 1160, 1271, 2537, 2603, 2888
PflMI CCANNNNNTGG 2 cut(s) 1479, 2061
PfoI TCCNGGA 1 cut(s) 2742
PleI GAGTC 8 cut(s) 143, 1058, 1153, 1204, 1213, 1910, 2296, 2758
PluTI GGCGCC 1 cut(s) 1623
PmaCI CACGTG 2 cut(s) 1305, 1437
PmlI CACGTG 2 cut(s) 1305, 1437
PpsI GAGTC 8 cut(s) 143, 1058, 1153, 1204, 1213, 1910, 2296, 2758
Ppu21I YACGTR 3 cut(s) 1305, 1437, 2212
PpuMI RGGWCCY 1 cut(s) 2500
Psp5II RGGWCCY 1 cut(s) 2500
Psp6I CCWGG 3 cut(s) 1440, 2204, 2722
PspCI CACGTG 2 cut(s) 1305, 1437
PspFI CCCAGC 2 cut(s) 1050, 1862
PspGI CCWGG 3 cut(s) 1440, 2204, 2722
PspN4I GGNNCC 8 cut(s) 622, 1134, 1332, 1431, 1432, 1446, 1621, 2241
PspOMI GGGCCC 1 cut(s) 1430
PspPI GGNCC 9 cut(s) 211, 1133, 1331, 1430, 1431, 1444, 1546, 1959, 2500
PspPPI RGGWCCY 1 cut(s) 2500
PsrI GAACNNNNNNTAC 2 cut(s) 370, 402
PstI CTGCAG 3 cut(s) 1834, 2301, 2491
PstNI CAGNNNCTG 1 cut(s) 1852
RsaI GTAC 9 cut(s) 382, 412, 975, 1631, 1927, 2210, 2214, 2241, 2694
RsaNI GTAC 9 cut(s) 381, 411, 974, 1630, 1926, 2209, 2213, 2240, 2693
RseI CAYNNNNRTG 3 cut(s) 89, 2411, 2522
SacII CCGCGG 1 cut(s) 433
SalI GTCGAC 1 cut(s) 1222
SaqAI TTAA 7 cut(s) 14, 276, 2094, 2318, 2505, 2667, 2896
Sau96I GGNCC 9 cut(s) 211, 1133, 1331, 1430, 1431, 1444, 1546, 1959, 2500
ScaI AGTACT 2 cut(s) 382, 412
SchI GAGTC 8 cut(s) 144, 1058, 1154, 1205, 1214, 1911, 2296, 2759
SduI GDGCHC 7 cut(s) 160, 575, 1404, 1434, 1680, 2385, 2759
SfcI CTRYAG 4 cut(s) 1830, 2219, 2297, 2487
SfoI GGCGCC 1 cut(s) 1621
Sfr303I CCGCGG 1 cut(s) 433
SgrBI CCGCGG 1 cut(s) 433
SinI GGWCC 4 cut(s) 1133, 1331, 1444, 2500
SmaI CCCGGG 2 cut(s) 309, 1963
SmiMI CAYNNNNRTG 3 cut(s) 89, 2411, 2522
SmlI CTYRAG 2 cut(s) 1788, 1805
SmoI CTYRAG 2 cut(s) 1788, 1805
SnaBI TACGTA 1 cut(s) 2212
SpeI ACTAGT 1 cut(s) 383
SphI GCATGC 1 cut(s) 2233
SspDI GGCGCC 1 cut(s) 1619
SspI AATATT 1 cut(s) 271
SspMI CTAG 6 cut(s) 8, 384, 456, 1040, 1683, 2289
StyI CCWWGG 1 cut(s) 1907
TaaI ACNGT 7 cut(s) 67, 927, 1186, 1280, 1347, 2120, 2815
TatI WGTACW 4 cut(s) 380, 410, 973, 1629
TfiI GAWTC 9 cut(s) 452, 584, 863, 986, 1160, 1271, 2537, 2603, 2888
Tru1I TTAA 7 cut(s) 14, 276, 2094, 2318, 2505, 2667, 2896
Tru9I TTAA 7 cut(s) 14, 276, 2094, 2318, 2505, 2667, 2896
TscAI CASTG 7 cut(s) 96, 534, 1189, 1421, 2049, 2390, 2848
TseFI GTSAC 2 cut(s) 67, 1321
Tsp45I GTSAC 2 cut(s) 67, 1321
TspDTI ATGAA 9 cut(s) 73, 378, 579, 843, 1600, 1791, 2429, 2534, 2630
TspGWI ACGGA 2 cut(s) 1311, 1473
TspMI CCCGGG 2 cut(s) 307, 1961
TspRI CASTG 7 cut(s) 96, 534, 1189, 1421, 2049, 2390, 2848
Van91I CCANNNNNTGG 2 cut(s) 1479, 2061
VpaK11BI GGWCC 4 cut(s) 1133, 1331, 1444, 2500
XapI RAATTY 5 cut(s) 49, 824, 850, 2022, 2583
XceI RCATGY 1 cut(s) 2233
XcmI CCANNNNNNNNNTGG 1 cut(s) 410
XmaI CCCGGG 2 cut(s) 307, 1961
XmiI GTMKAC 2 cut(s) 1223, 2115
XspI CTAG 6 cut(s) 8, 384, 456, 1040, 1683, 2289
ZrmI AGTACT 2 cut(s) 382, 412
Zsp2I ATGCAT 1 cut(s) 2231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.