Rroxscaffold_2G00142420

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011678
Physical Location & Seq
Reverse (-)
80471127 .. 80475325
4199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00142420.1

Sequence Viewer

Length: 2379 bp
ATGGCGTTGGGGAATAGGTCGCGCAGTAGCAGTAACAAGCGGTCGTCTTCAAGCTCTTACACATCAACCGTGATGGTAATGGGATTCATAGCACTGTGTGTGGTTGGTGTGTGGATGATGAATTACTCCAACGCTGGAGTTTCAACACAAACCACCACGGCTCGAACCAACACCGTCACTTCCACTGCCTCCTCGGATGAAAACAATACACAAGCCGCTGAGAATAACAATAATAACAATGAGAAACCTAAGGCCTTTGAAGACAATCCCGGTGATCTTCCGGACGATGCTATAAAAAATGATGATGGGCCTAACAAATCGGATGCTGCTGCTATCAACGAGGGAAAGACGTCCAGAGAAAAACAAGAGCAAGCTGCAGCTGAAGAACAGAAAGAGGATCAGAAGCAGCAAGAGCTTAACCAAGCACAGTTATCTGAGGAAACCACTATACCTGAAAACCAACAAGCTGAACAAACCGCCACACAAGAGAAGATCATCACTGCTGATGATCAACCTGACGAGAAAAACCACCAACAAAAGAACAATAATGGCGGCCAGAGCGGTGGAAATGATCAATTAGATAATCAACAAAAGAAAAACCCAGGAGAACAACAACAACAACAACAACAACAGCAACAAGAAGAATCTTACCCTCAGCGAAAGAAAGGTAATGCAGCAGCTGATCAAAACAATAAGGGCACCGAGTCCAACTCGGGTGAAACGCTACTCAACGGCGGGGACAGCTCGGGCATTCCGAAAGAGTCAAAGGAGTCGAAGAAGTCGTGGAGGACTCAAGCGGATCAGTCGGAGAACGAGAAGGAGAGGCGCAAGGACGAAAAGGAGGGTCAAGATAGCATCTACGGGTACACGTGGCAGCTTTGCAATGTGACTACAGGTCCTGATTATATACCTTGTTTGGACAATGAGAAGGCTATAAAGAAGCTGCGTTCTACCAAACACTTCCAGCATCGAGAGAGGCATTGTCCGCAGGAGGGTCCTACTTGCCTGGTTCCACATCCGGAGGGTTACAAGACGTCGATCCAGTGGCCGAAAAGCAGAGACAAGATATGGTATCACAATGTACCGCACACATTGCTGGCGGAGGTGAAGGGACACCAGAACTGGGTTAAGGTGTCCGTACCGAATCTAGCATGGGGAAAGCACACTCGAGTTATATTGGACGTTGGGTGTGGTGTTGCGAGCTTTGGTGGTTATCTCTTTGACAGAGATGTTCTTACAATGTCTTTTGCACCCAAAGATGAGCATGAAGCTCAAGTTCAGTTTGCCCTGGAGAGGGGTATTCCAGCCATTTCAGCAGTCATGGGTACTCAGAGGCTGCCCTTCCCCAGCAGGGTCTTTGATGCCGTCCACTGTGCACGTTGCAGAGTGCCTTGGCATCTCGAAGGGGGAATGCTTCTTTTGGAGTTGAATCGTGTTTTAAGGCCTGGGGGTTACTTCATTTGGTCAGCCACTCCTGTTTATGGGAAGCTTGACGAAGACAAAAAAATATGGAAAGAAATGACTGCCTTAACAAAGTCTATGTGTTGGGACCTTGTTACGATCAAGAACAACAAAGTAAATTTAGTTGCGGCTGCCATCTACCGCAAGCCAACCTCGAACCAATGCTACGAACAAAGAAAGCAAAAGCAACCTCCTATGTGCAAAACCGATGATGATCCCAATGCTGCCTGGTTGTATGTACCTCTGCAGGCATGTATGCACAGGGTTCCTGTTGACAAATCTGAGAGAGGGACTCAATGGCCTGAGAACTGGCCTAATCGACTGGAAACTCCTCCTTACTGGTTAAACAGCTCCCGGATGGGTATTTATGGAAAACCAGCACCCCAAGATTTTGCAAGAGATTATGAACACTGGAAACGTGTGATCAACAACAGTTACATGAAAAGTTTGGGTATCAATTGGTCCGGAGTCAGAAATGTCATGGATATGAGAGCGGTTTATGGAGGGTTTGCCGCCGCTCTCAAGGATCTTAAGGTCTGGGTGATGAATGTGGTGAATGTAGATTCTGCAGATACTCTCCCCATCATCTATGAACGTGGTCTTTTTGGAATTTATCATGATTGGTGTGAATCTTTTAGCACCTACCCAAGAACCTATGACCTCCTCCATGCAGATCATCTCTTTTCTTCGCTCAAAAAGAGGTGCAGACTTCCTCCGGTAATGGCAGAAATTGATCGGATTGTCAGGCCGGGAGGCAAATTGATCGTCCGCGATGAATCCAGCGCAATTGGGGAGGTTGAGAACTTGTTGAAGTCCCTGCACTGGGAGGTTCACTTAACCTTCTCCAAAAACCAAGAAGGGATGCTTAGTGCACAGAAAGGCAATTGGCGGCCAACCGCATTGGCAGATACTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

792

Amino Acids

89.46

Weight (kDa)

6.85

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 301 - 379 4.6e-31 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_29 PF03141 380 - 779 1.9e-176 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11 PF08241 393 - 487 1.6e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011678)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51070 AT3G51070
fragaria_vesca FvH4_1g12411
malus_domestica MD02G1136800.v1.1 MD15G1250600.v1.1
prunus_persica Prupe.7G163700_v2.0.a1
pyrus_communis pycom15g22090
rosa_chinensis RchiOBHm_Chr2g0100281
rosa_laevigata RLG00000016952
rosa_multiflora Rmu_sc0002547.1_g000021 Rmu_sc0002547.1_g000022
rosa_roxburghii Rroxscaffold_2G00142420
rosa_rugosa Rorug02G0087100 Rorug02G0087200
rosa_samantha Rh2AG136100 Rh2BG139700 Rh2CG141400 Rh2DG141600
rosa_wichuraiana Rw2G010650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 353, 1037
AccB1I GGYRCC 1 cut(s) 698
AccBSI CCGCTC 3 cut(s) 561, 1955, 1979
AccII CGCG 2 cut(s) 22, 2232
AccIII TCCGGA 3 cut(s) 280, 1018, 1925
AclWI GGATC 5 cut(s) 405, 807, 1033, 1670, 1995
AcoI YGGCCR 3 cut(s) 553, 1046, 2351
AcsI RAATTY 2 cut(s) 1579, 2070
AcuI CTGAAG 1 cut(s) 402
AcvI CACGTG 1 cut(s) 870
AcyI GRCGYC 2 cut(s) 350, 1034
AdeI CACNNNGTG 1 cut(s) 98
AfaI GTAC 5 cut(s) 866, 1083, 1140, 1327, 1701
AfiI CCNNNNNNNGG 8 cut(s) 992, 1123, 1293, 1294, 1351, 1481, 2283, 2284
AflII CTTAAG 1 cut(s) 1991
AflIII ACRYGT 2 cut(s) 867, 1879
AgsI TTSAA 5 cut(s) 51, 144, 260, 1429, 2272
AhdI GACNNNNNGTC 1 cut(s) 894
AjnI CCWGG 5 cut(s) 601, 1005, 1287, 1444, 1688
Alw21I GWGCWC 2 cut(s) 1378, 2335
Alw26I GTCTC 1 cut(s) 1053
Alw44I GTGCAC 2 cut(s) 1374, 2331
AlwI GGATC 5 cut(s) 405, 807, 1033, 1670, 1995
AlwNI CAGNNNCTG 3 cut(s) 680, 899, 1336
Ama87I CYCGRG 3 cut(s) 712, 745, 1167
Aor13HI TCCGGA 3 cut(s) 280, 1018, 1925
AoxI GGCC 9 cut(s) 252, 308, 553, 1046, 1442, 1760, 1772, 2207, 2351
ApaLI GTGCAC 2 cut(s) 1374, 2331
ApoI RAATTY 2 cut(s) 1579, 2070
ArsI GACNNNNNNTTYG 2 cut(s) 2211, 2243
AspLEI GCGC 3 cut(s) 24, 828, 2246
AspS9I GGNCC 5 cut(s) 308, 896, 995, 1549, 1923
AsuC2I CCSGG 3 cut(s) 270, 1816, 2211
AsuHPI GGTGA 5 cut(s) 284, 728, 1117, 2014, 2026
AvaI CYCGRG 3 cut(s) 712, 745, 1167
AvaII GGWCC 4 cut(s) 896, 995, 1549, 1923
AxyI CCTNAGG 1 cut(s) 249
BaeGI GKGCMC 3 cut(s) 701, 1378, 2335
BanI GGYRCC 1 cut(s) 698
BbrPI CACGTG 1 cut(s) 870
BbsI GAAGAC 3 cut(s) 39, 267, 1503
Bbv12I GWGCWC 2 cut(s) 1378, 2335
BbvCI CCTCAGC 1 cut(s) 654
BccI CCATC 5 cut(s) 67, 299, 1604, 1813, 2051
BceAI ACGGC 3 cut(s) 174, 748, 1349
BcgI CGANNNNNNTGC 2 cut(s) 2206, 2240
BciT130I CCWGG 5 cut(s) 603, 1007, 1289, 1446, 1690
BclI TGATCA 4 cut(s) 508, 571, 682, 1884
BcnI CCSGG 3 cut(s) 270, 1816, 2211
BcoDI GTCTC 1 cut(s) 1053
BfaI CTAG 1 cut(s) 1148
BfmI CTRYAG 4 cut(s) 375, 891, 1706, 2028
BfrI CTTAAG 1 cut(s) 1991
Bme1390I CCNGG 8 cut(s) 270, 603, 1007, 1289, 1446, 1690, 1816, 2211
Bme18I GGWCC 4 cut(s) 896, 995, 1549, 1923
BmeRI GACNNNNNGTC 1 cut(s) 894
BmeT110I CYCGRG 3 cut(s) 712, 745, 1167
BmgT120I GGNCC 5 cut(s) 308, 896, 995, 1549, 1923
BmiI GGNNCC 5 cut(s) 700, 996, 1011, 1550, 1728
BmrFI CCNGG 8 cut(s) 270, 603, 1007, 1289, 1446, 1690, 1816, 2211
BmrI ACTGGG 2 cut(s) 1132, 2293
BmsI GCATC 7 cut(s) 277, 313, 864, 976, 1351, 1405, 2313
BmuI ACTGGG 2 cut(s) 1132, 2293
BpiI GAAGAC 3 cut(s) 39, 267, 1503
BplI GAGNNNNNCTC 4 cut(s) 695, 727, 1738, 1770
BpmI CTGGAG 2 cut(s) 156, 1310
Bpu10I CCTNAGC 1 cut(s) 654
BpuEI CTTGAG 3 cut(s) 777, 1257, 1967
BpuMI CCSGG 3 cut(s) 270, 1816, 2211
BsaAI YACGTR 1 cut(s) 870
BsaBI GATNNNNATC 1 cut(s) 1674
BsaHI GRCGYC 2 cut(s) 350, 1034
BsaJI CCNNGG 6 cut(s) 156, 192, 601, 1287, 1391, 1445
BsaWI WCCGGW 4 cut(s) 280, 1018, 1925, 2176
Bsc4I CCNNNNNNNGG 8 cut(s) 992, 1123, 1293, 1294, 1351, 1481, 2283, 2284
Bse1I ACTGG 7 cut(s) 1042, 1127, 1775, 1788, 1805, 1877, 2288
Bse21I CCTNAGG 1 cut(s) 249
Bse3DI GCAATG 2 cut(s) 889, 1091
Bse8I GATNNNNATC 1 cut(s) 1674
BseAI TCCGGA 3 cut(s) 280, 1018, 1925
BseBI CCWGG 5 cut(s) 603, 1007, 1289, 1446, 1690
BseDI CCNNGG 6 cut(s) 156, 192, 601, 1287, 1391, 1445
BseGI GGATG 6 cut(s) 120, 202, 328, 1015, 1824, 2328
BseJI GATNNNNATC 1 cut(s) 1674
BseLI CCNNNNNNNGG 8 cut(s) 992, 1123, 1293, 1294, 1351, 1481, 2283, 2284
BseMI GCAATG 2 cut(s) 889, 1091
BseMII CTCAG 6 cut(s) 210, 426, 668, 1343, 1734, 1755
BseNI ACTGG 7 cut(s) 1042, 1127, 1775, 1788, 1805, 1877, 2288
BseRI GAGGAG 3 cut(s) 181, 1782, 2114
BseSI GKGCMC 3 cut(s) 701, 1378, 2335
BseYI CCCAGC 1 cut(s) 1346
BsgI GTGCAG 2 cut(s) 2185, 2264
Bsh1236I CGCG 2 cut(s) 22, 2232
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 9 cut(s) 254, 310, 555, 1048, 1444, 1762, 1774, 2209, 2353
BshNI GGYRCC 1 cut(s) 698
BsiEI CGRYCG 1 cut(s) 44
BsiHKAI GWGCWC 2 cut(s) 1378, 2335
BsiHKCI CYCGRG 3 cut(s) 712, 745, 1167
BsiSI CCGG 7 cut(s) 270, 281, 1019, 1816, 1926, 2177, 2210
BslFI GGGAC 5 cut(s) 752, 1125, 1562, 1765, 2260
BslI CCNNNNNNNGG 8 cut(s) 992, 1123, 1293, 1294, 1351, 1481, 2283, 2284
BsmAI GTCTC 1 cut(s) 1053
BsmFI GGGAC 5 cut(s) 752, 1125, 1562, 1765, 2260
BsmI GAATGC 2 cut(s) 750, 1416
BsnI GGCC 9 cut(s) 254, 310, 555, 1048, 1444, 1762, 1774, 2209, 2353
BsoBI CYCGRG 3 cut(s) 712, 745, 1167
Bsp1286I GDGCHC 3 cut(s) 701, 1378, 2335
Bsp13I TCCGGA 3 cut(s) 280, 1018, 1925
BspANI GGCC 9 cut(s) 254, 310, 555, 1048, 1444, 1762, 1774, 2209, 2353
BspCNI CTCAG 6 cut(s) 211, 427, 667, 1342, 1735, 1756
BspEI TCCGGA 3 cut(s) 280, 1018, 1925
BspFNI CGCG 2 cut(s) 22, 2232
BspHI TCATGA 1 cut(s) 2077
BspLI GGNNCC 5 cut(s) 700, 996, 1011, 1550, 1728
BspMAI CTGCAG 3 cut(s) 379, 1710, 2032
BspPI GGATC 5 cut(s) 405, 807, 1033, 1670, 1995
BspT107I GGYRCC 1 cut(s) 698
BspTI CTTAAG 1 cut(s) 1991
BsrBI CCGCTC 3 cut(s) 561, 1955, 1979
BsrDI GCAATG 2 cut(s) 889, 1091
BsrI ACTGG 7 cut(s) 1042, 1127, 1775, 1788, 1805, 1877, 2288
BssECI CCNNGG 6 cut(s) 156, 192, 601, 1287, 1391, 1445
BssNI GRCGYC 2 cut(s) 350, 1034
BssT1I CCWWGG 1 cut(s) 1391
Bst2UI CCWGG 5 cut(s) 603, 1007, 1289, 1446, 1690
Bst4CI ACNGT 6 cut(s) 70, 96, 175, 429, 1373, 1895
BstACI GRCGYC 2 cut(s) 350, 1034
BstAFI CTTAAG 1 cut(s) 1991
BstAPI GCANNNNNTGC 1 cut(s) 1093
BstBAI YACGTR 1 cut(s) 870
BstC8I GCNNGC 5 cut(s) 372, 1098, 1201, 1607, 1710
BstDEI CTNAG 8 cut(s) 219, 249, 435, 654, 1329, 1743, 1764, 2327
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 6 cut(s) 120, 202, 328, 1015, 1824, 2328
BstFNI CGCG 2 cut(s) 22, 2232
BstHHI GCGC 3 cut(s) 24, 828, 2246
BstMAI GTCTC 1 cut(s) 1053
BstMCI CGRYCG 1 cut(s) 44
BstMWI GCNNNNNNNGC 6 cut(s) 412, 558, 741, 985, 1093, 1313
BstNI CCWGG 5 cut(s) 603, 1007, 1289, 1446, 1690
BstNSI RCATGY 1 cut(s) 1716
BstSCI CCNGG 8 cut(s) 268, 601, 1005, 1287, 1444, 1688, 1814, 2209
BstSFI CTRYAG 4 cut(s) 375, 891, 1706, 2028
BstSLI GKGCMC 3 cut(s) 701, 1378, 2335
BstUI CGCG 2 cut(s) 22, 2232
BstV2I GAAGAC 3 cut(s) 39, 267, 1503
BstX2I RGATCY 1 cut(s) 1987
BstXI CCANNNNNNTGG 1 cut(s) 563
BstYI RGATCY 1 cut(s) 1987
Bsu36I CCTNAGG 1 cut(s) 249
BsuRI GGCC 9 cut(s) 254, 310, 555, 1048, 1444, 1762, 1774, 2209, 2353
BtgI CCRYGG 1 cut(s) 156
BtgZI GCGATG 1 cut(s) 2247
BtsCI GGATG 6 cut(s) 120, 202, 328, 1015, 1824, 2328
BtsI GCAGTG 2 cut(s) 183, 498
BtsIMutI CAGTG 7 cut(s) 92, 183, 498, 1049, 1369, 1870, 2281
Cac8I GCNNGC 5 cut(s) 372, 1098, 1201, 1607, 1710
CaiI CAGNNNCTG 3 cut(s) 680, 899, 1336
CciI TCATGA 1 cut(s) 2077
CfoI GCGC 3 cut(s) 24, 828, 2246
Cfr13I GGNCC 5 cut(s) 308, 896, 995, 1549, 1923
Csp6I GTAC 5 cut(s) 865, 1082, 1139, 1326, 1700
CviAII CATG 8 cut(s) 1152, 1265, 1321, 1713, 1900, 1942, 2078, 2129
CviQI GTAC 5 cut(s) 865, 1082, 1139, 1326, 1700
DdeI CTNAG 8 cut(s) 219, 249, 435, 654, 1329, 1743, 1764, 2327
DraIII CACNNNGTG 1 cut(s) 98
DriI GACNNNNNGTC 1 cut(s) 894
EaeI YGGCCR 3 cut(s) 553, 1046, 2351
Eam1105I GACNNNNNGTC 1 cut(s) 894
EciI GGCGGA 1 cut(s) 1115
Eco130I CCWWGG 1 cut(s) 1391
Eco147I AGGCCT 2 cut(s) 254, 1444
Eco47I GGWCC 4 cut(s) 896, 995, 1549, 1923
Eco57I CTGAAG 1 cut(s) 402
Eco72I CACGTG 1 cut(s) 870
Eco81I CCTNAGG 1 cut(s) 249
Eco88I CYCGRG 3 cut(s) 712, 745, 1167
EcoO109I RGGNCCY 3 cut(s) 896, 995, 1549
EcoRII CCWGG 5 cut(s) 601, 1005, 1287, 1444, 1688
EcoT14I CCWWGG 1 cut(s) 1391
ErhI CCWWGG 1 cut(s) 1391
FaeI CATG 8 cut(s) 1155, 1268, 1324, 1716, 1903, 1945, 2081, 2132
FalI AAGNNNNNCTT 2 cut(s) 2310, 2342
FaqI GGGAC 5 cut(s) 752, 1125, 1562, 1765, 2260
FatI CATG 8 cut(s) 1151, 1264, 1320, 1712, 1899, 1941, 2077, 2128
FauI CCCGC 1 cut(s) 728
FbaI TGATCA 4 cut(s) 508, 571, 682, 1884
FokI GGATG 6 cut(s) 127, 209, 335, 1002, 1831, 2335
FspBI CTAG 1 cut(s) 1148
GlaI GCGC 3 cut(s) 23, 827, 2245
GsaI CCCAGC 1 cut(s) 1350
GsuI CTGGAG 2 cut(s) 156, 1310
HaeIII GGCC 9 cut(s) 254, 310, 555, 1048, 1444, 1762, 1774, 2209, 2353
HapII CCGG 7 cut(s) 270, 281, 1019, 1816, 1926, 2177, 2210
HhaI GCGC 3 cut(s) 24, 828, 2246
Hin1I GRCGYC 2 cut(s) 350, 1034
Hin1II CATG 8 cut(s) 1155, 1268, 1324, 1716, 1903, 1945, 2081, 2132
Hin6I GCGC 3 cut(s) 22, 826, 2244
HinP1I GCGC 3 cut(s) 22, 826, 2244
HincII GTYRAC 1 cut(s) 1735
HindII GTYRAC 1 cut(s) 1735
HindIII AAGCTT 1 cut(s) 1487
HpaII CCGG 7 cut(s) 270, 281, 1019, 1816, 1926, 2177, 2210
HphI GGTGA 5 cut(s) 284, 728, 1117, 2014, 2026
Hpy166II GTNNAC 6 cut(s) 867, 1369, 1376, 1735, 2293, 2333
Hpy8I GTNNAC 6 cut(s) 867, 1369, 1376, 1735, 2293, 2333
Hpy99I CGWCG 1 cut(s) 1039
HpyAV CCTTC 7 cut(s) 811, 922, 1102, 1351, 1397, 2311, 2312
HpyCH4III ACNGT 6 cut(s) 70, 96, 175, 429, 1373, 1895
HpyCH4IV ACGT 7 cut(s) 350, 869, 1034, 1182, 1378, 1879, 2056
HpyF10VI GCNNNNNNNGC 6 cut(s) 412, 558, 741, 985, 1093, 1313
HpyF3I CTNAG 8 cut(s) 219, 249, 435, 654, 1329, 1743, 1764, 2327
HpySE526I ACGT 7 cut(s) 350, 869, 1034, 1182, 1378, 1879, 2056
Hsp92I GRCGYC 2 cut(s) 350, 1034
Hsp92II CATG 8 cut(s) 1155, 1268, 1324, 1716, 1903, 1945, 2081, 2132
HspAI GCGC 3 cut(s) 22, 826, 2244
Kpn2I TCCGGA 3 cut(s) 280, 1018, 1925
Ksp22I TGATCA 4 cut(s) 508, 571, 682, 1884
LmnI GCTCC 1 cut(s) 1817
LweI GCATC 7 cut(s) 277, 313, 864, 976, 1351, 1405, 2313
MaeI CTAG 1 cut(s) 1148
MaeII ACGT 7 cut(s) 350, 869, 1034, 1182, 1378, 1879, 2056
MaeIII GTNAC 7 cut(s) 32, 175, 886, 1025, 1451, 1555, 1895
MbiI CCGCTC 3 cut(s) 561, 1955, 1979
MboII GAAGA 9 cut(s) 39, 269, 272, 395, 502, 653, 787, 1508, 2139
MfeI CAATTG 3 cut(s) 1918, 2247, 2344
MflI RGATCY 1 cut(s) 1987
MhlI GDGCHC 3 cut(s) 701, 1378, 2335
MluCI AATT 9 cut(s) 121, 575, 1579, 1918, 2070, 2190, 2219, 2247, 2344
MlyI GAGTC 6 cut(s) 713, 770, 779, 784, 1747, 1938
MmeI TCCRAC 3 cut(s) 153, 732, 786
MroI TCCGGA 3 cut(s) 280, 1018, 1925
MseI TTAA 8 cut(s) 417, 1128, 1439, 1529, 1805, 1992, 2297, 2377
MslI CAYNNNNRTG 1 cut(s) 1946
MspA1I CMGCKG 3 cut(s) 218, 380, 680
MspCI CTTAAG 1 cut(s) 1991
MspI CCGG 7 cut(s) 270, 281, 1019, 1816, 1926, 2177, 2210
MspR9I CCNGG 8 cut(s) 270, 603, 1007, 1289, 1446, 1690, 1816, 2211
MunI CAATTG 3 cut(s) 1918, 2247, 2344
Mva1269I GAATGC 2 cut(s) 750, 1416
MvaI CCWGG 5 cut(s) 603, 1007, 1289, 1446, 1690
MvnI CGCG 2 cut(s) 22, 2232
MwoI GCNNNNNNNGC 6 cut(s) 412, 558, 741, 985, 1093, 1313
NciI CCSGG 3 cut(s) 270, 1816, 2211
NlaIII CATG 8 cut(s) 1155, 1268, 1324, 1716, 1903, 1945, 2081, 2132
NlaIV GGNNCC 5 cut(s) 700, 996, 1011, 1550, 1728
NmuCI GTSAC 2 cut(s) 175, 886
NspI RCATGY 1 cut(s) 1716
PaeR7I CTCGAG 1 cut(s) 1167
PagI TCATGA 1 cut(s) 2077
PceI AGGCCT 2 cut(s) 254, 1444
PcsI WCGNNNNNNNCGW 2 cut(s) 752, 779
PctI GAATGC 2 cut(s) 750, 1416
PfeI GAWTC 7 cut(s) 84, 644, 1144, 1429, 2024, 2090, 2237
PfoI TCCNGGA 1 cut(s) 1814
PleI GAGTC 6 cut(s) 712, 769, 778, 784, 1747, 1937
PmaCI CACGTG 1 cut(s) 870
PmlI CACGTG 1 cut(s) 870
PpsI GAGTC 6 cut(s) 712, 769, 778, 784, 1747, 1937
Ppu21I YACGTR 1 cut(s) 870
PpuMI RGGWCCY 3 cut(s) 896, 995, 1549
Psp5II RGGWCCY 3 cut(s) 896, 995, 1549
Psp6I CCWGG 5 cut(s) 601, 1005, 1287, 1444, 1688
PspCI CACGTG 1 cut(s) 870
PspFI CCCAGC 1 cut(s) 1346
PspGI CCWGG 5 cut(s) 601, 1005, 1287, 1444, 1688
PspN4I GGNNCC 5 cut(s) 700, 996, 1011, 1550, 1728
PspPI GGNCC 5 cut(s) 308, 896, 995, 1549, 1923
PspPPI RGGWCCY 3 cut(s) 896, 995, 1549
PspXI VCTCGAGB 1 cut(s) 1167
PsrI GAACNNNNNNTAC 2 cut(s) 1610, 1642
PstI CTGCAG 3 cut(s) 379, 1710, 2032
PstNI CAGNNNCTG 3 cut(s) 680, 899, 1336
PsuI RGATCY 1 cut(s) 1987
PvuII CAGCTG 2 cut(s) 380, 680
RsaI GTAC 5 cut(s) 866, 1083, 1140, 1327, 1701
RsaNI GTAC 5 cut(s) 865, 1082, 1139, 1326, 1700
RseI CAYNNNNRTG 1 cut(s) 1946
SaqAI TTAA 8 cut(s) 417, 1128, 1439, 1529, 1805, 1992, 2297, 2377
Sau96I GGNCC 5 cut(s) 308, 896, 995, 1549, 1923
SchI GAGTC 6 cut(s) 713, 770, 779, 784, 1747, 1938
ScrFI CCNGG 8 cut(s) 270, 603, 1007, 1289, 1446, 1690, 1816, 2211
SduI GDGCHC 3 cut(s) 701, 1378, 2335
SfaNI GCATC 7 cut(s) 277, 313, 864, 976, 1351, 1405, 2313
SfcI CTRYAG 4 cut(s) 375, 891, 1706, 2028
Sfr274I CTCGAG 1 cut(s) 1167
SinI GGWCC 4 cut(s) 896, 995, 1549, 1923
SlaI CTCGAG 1 cut(s) 1167
SmiMI CAYNNNNRTG 1 cut(s) 1946
SmlI CTYRAG 5 cut(s) 792, 1167, 1272, 1982, 1991
SmoI CTYRAG 5 cut(s) 792, 1167, 1272, 1982, 1991
Sse9I AATT 9 cut(s) 121, 575, 1579, 1918, 2070, 2190, 2219, 2247, 2344
SseBI AGGCCT 2 cut(s) 254, 1444
SspMI CTAG 1 cut(s) 1148
StuI AGGCCT 2 cut(s) 254, 1444
StyD4I CCNGG 8 cut(s) 268, 601, 1005, 1287, 1444, 1688, 1814, 2209
StyI CCWWGG 1 cut(s) 1391
TaaI ACNGT 6 cut(s) 70, 96, 175, 429, 1373, 1895
TaiI ACGT 7 cut(s) 353, 872, 1037, 1185, 1381, 1882, 2059
TaqI TCGA 8 cut(s) 163, 773, 970, 1037, 1168, 1401, 1616, 1780
TasI AATT 9 cut(s) 121, 575, 1579, 1918, 2070, 2190, 2219, 2247, 2344
TauI GCSGC 6 cut(s) 218, 555, 1592, 1976, 1979, 2353
TfiI GAWTC 7 cut(s) 84, 644, 1144, 1429, 2024, 2090, 2237
Tru1I TTAA 8 cut(s) 417, 1128, 1439, 1529, 1805, 1992, 2297, 2377
Tru9I TTAA 8 cut(s) 417, 1128, 1439, 1529, 1805, 1992, 2297, 2377
TscAI CASTG 7 cut(s) 99, 190, 505, 1049, 1376, 1877, 2288
TseFI GTSAC 2 cut(s) 175, 886
Tsp45I GTSAC 2 cut(s) 175, 886
TspGWI ACGGA 1 cut(s) 1126
TspRI CASTG 7 cut(s) 99, 190, 505, 1049, 1376, 1877, 2288
Vha464I CTTAAG 1 cut(s) 1991
VneI GTGCAC 2 cut(s) 1374, 2331
VpaK11BI GGWCC 4 cut(s) 896, 995, 1549, 1923
XapI RAATTY 2 cut(s) 1579, 2070
XceI RCATGY 1 cut(s) 1716
XhoI CTCGAG 1 cut(s) 1167
XspI CTAG 1 cut(s) 1148
ZraI GACGTC 2 cut(s) 351, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.