MD02G1144200.v1.1

Soluble inorganic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016505
Physical Location & Seq
Reverse (-)
11961527 .. 11965035
3509 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1144200.v1.1.491

Sequence Viewer

Length: 564 bp
ATGTCAAGGAGATCTGTTGCTGCTCATCCCTGGCATGACTTAGAGATTGGACCTGGTGCACCAGCAGTTTTCAACTGTGTTGTTGAAATTGGCAAAGGCAGCAAGGTTAAGTATGAGCTCGACAAGACAACTGGCCTTATAGTAGTTGACCGTGTGCTTTACTCATCGGTTGTTTACCCGCACAACTATGGTTTCATCCCACGAACAATCTGTGAGGATAGTGATCCAATGGATGTCCTAGTACTGATGCAGGAGCCCGTGATACCTGGTTCTTTCCTTCGTGCTCGCGCTATTGGATTAATGCCCATGATTGATCAAGGTGAAAAGGATGACAAGATTATTGCAGTATGTGCTGATGACCCTGAGTTCCGCCATATCACTGACCTCAAGGAGCTTCCTCCACATCGCCTTGCAGAAATTCGTCGGTTCTTTGAGGACTACAAAAAGAATGAGAACAAGGAAGTTGAGGTTGAAGACTTTCTACCGGCTGAGGCTGCCATCGAGGCCATCAGATACTCCATGAACCTGTATGCATCTTACATCGTGGAGAGCTTGAGGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004427 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Pyrophosphatase

Protein Analysis

188

Amino Acids

21.25

Weight (kDa)

5.07

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyrophosphatase PF00719 26 - 177 9.7e-57 Inorganic pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18230
fragaria_vesca FvH4_1g12970 FvH4_1g12970
malus_domestica MD02G1144200.v1.1
prunus_persica Prupe.7G157400_v2.0.a1
pyrus_communis pycom02g11410
rosa_chinensis RchiOBHm_Chr2g0101021
rosa_laevigata RLG00000017038
rosa_roxburghii Rroxscaffold_2G00141630
rosa_rugosa Rorug02G0095400
rosa_samantha Rh2BG147700 Rh2CG148900 Rh2DG148200
rosa_wichuraiana Rw2G011190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 288
AciI CCGC 2 cut(s) 179, 370
AclWI GGATC 1 cut(s) 218
AcsI RAATTY 1 cut(s) 417
AfaI GTAC 1 cut(s) 243
AgsI TTSAA 3 cut(s) 73, 86, 473
AjnI CCWGG 3 cut(s) 29, 52, 265
AluBI AGCT 3 cut(s) 118, 394, 552
AluI AGCT 3 cut(s) 118, 394, 552
Alw21I GWGCWC 3 cut(s) 61, 120, 286
Alw44I GTGCAC 1 cut(s) 57
AlwI GGATC 1 cut(s) 218
AoxI GGCC 2 cut(s) 133, 504
ApaLI GTGCAC 1 cut(s) 57
ApeKI GCWGC 3 cut(s) 20, 99, 494
ApoI RAATTY 1 cut(s) 417
AseI ATTAAT 1 cut(s) 299
Asp700I GAANNNNTTC 1 cut(s) 477
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 50
AsuHPI GGTGA 1 cut(s) 332
AvaII GGWCC 1 cut(s) 50
BaeGI GKGCMC 1 cut(s) 61
BanII GRGCYC 2 cut(s) 120, 258
BarI GAAGNNNNNNTAC 2 cut(s) 465, 497
BbsI GAAGAC 1 cut(s) 480
Bbv12I GWGCWC 3 cut(s) 61, 120, 286
BbvCI CCTCAGC 1 cut(s) 489
BbvI GCAGC 3 cut(s) 7, 111, 481
BccI CCATC 2 cut(s) 506, 515
BciT130I CCWGG 3 cut(s) 31, 54, 267
BclI TGATCA 1 cut(s) 313
BfaI CTAG 1 cut(s) 239
BglI GCCNNNNNGGC 1 cut(s) 503
BglII AGATCT 1 cut(s) 11
BisI GCNGC 3 cut(s) 21, 100, 495
BlsI GCNGC 3 cut(s) 22, 101, 496
BmcAI AGTACT 1 cut(s) 243
Bme1390I CCNGG 3 cut(s) 31, 54, 267
Bme18I GGWCC 1 cut(s) 50
BmgT120I GGNCC 1 cut(s) 50
BmiI GGNNCC 1 cut(s) 255
BmrFI CCNGG 3 cut(s) 31, 54, 267
BmsI GCATC 2 cut(s) 237, 542
BpiI GAAGAC 1 cut(s) 480
Bpu10I CCTNAGC 1 cut(s) 489
BpuEI CTTGAG 1 cut(s) 371
BsaBI GATNNNNATC 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 29
Bse118I RCCGGY 1 cut(s) 484
Bse1I ACTGG 1 cut(s) 136
Bse8I GATNNNNATC 1 cut(s) 222
BseBI CCWGG 3 cut(s) 31, 54, 267
BseDI CCNNGG 1 cut(s) 29
BseGI GGATG 4 cut(s) 25, 195, 238, 334
BseJI GATNNNNATC 1 cut(s) 222
BseMII CTCAG 2 cut(s) 354, 480
BseNI ACTGG 1 cut(s) 136
BseSI GKGCMC 1 cut(s) 61
BseXI GCAGC 3 cut(s) 7, 111, 481
Bsh1236I CGCG 1 cut(s) 288
BshFI GGCC 2 cut(s) 135, 506
BsiHKAI GWGCWC 3 cut(s) 61, 120, 286
BsiSI CCGG 1 cut(s) 485
BsnI GGCC 2 cut(s) 135, 506
Bsp1286I GDGCHC 4 cut(s) 61, 120, 258, 286
Bsp143I GATC 3 cut(s) 11, 223, 313
BspACI CCGC 2 cut(s) 179, 370
BspANI GGCC 2 cut(s) 135, 506
BspCNI CTCAG 2 cut(s) 355, 481
BspFNI CGCG 1 cut(s) 288
BspLI GGNNCC 1 cut(s) 255
BspPI GGATC 1 cut(s) 218
BsrFI RCCGGY 1 cut(s) 484
BsrI ACTGG 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 484
BssECI CCNNGG 1 cut(s) 29
BssMI GATC 3 cut(s) 11, 223, 313
Bst2UI CCWGG 3 cut(s) 31, 54, 267
Bst4CI ACNGT 2 cut(s) 77, 152
BstAPI GCANNNNNTGC 1 cut(s) 350
BstC8I GCNNGC 1 cut(s) 286
BstDEI CTNAG 3 cut(s) 40, 363, 489
BstF5I GGATG 4 cut(s) 25, 195, 238, 334
BstFNI CGCG 1 cut(s) 288
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 3 cut(s) 14, 226, 316
BstMBI GATC 3 cut(s) 11, 223, 313
BstMWI GCNNNNNNNGC 4 cut(s) 99, 350, 494, 503
BstNI CCWGG 3 cut(s) 31, 54, 267
BstSCI CCNGG 3 cut(s) 29, 52, 265
BstSLI GKGCMC 1 cut(s) 61
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 3 cut(s) 7, 111, 481
BstV2I GAAGAC 1 cut(s) 480
BstX2I RGATCY 1 cut(s) 11
BstYI RGATCY 1 cut(s) 11
BsuRI GGCC 2 cut(s) 135, 506
BtgZI GCGATG 1 cut(s) 389
BtsCI GGATG 4 cut(s) 25, 195, 238, 334
BtsIMutI CAGTG 1 cut(s) 378
Cac8I GCNNGC 1 cut(s) 286
CfoI GCGC 1 cut(s) 290
Cfr10I RCCGGY 1 cut(s) 484
Cfr13I GGNCC 1 cut(s) 50
CsiI ACCWGGT 2 cut(s) 52, 265
Csp6I GTAC 1 cut(s) 242
CviAII CATG 3 cut(s) 35, 307, 520
CviJI RGCY 8 cut(s) 118, 135, 256, 394, 488, 494, 506, 552
CviKI_1 RGCY 8 cut(s) 118, 135, 256, 394, 488, 494, 506, 552
CviQI GTAC 1 cut(s) 242
DdeI CTNAG 3 cut(s) 40, 363, 489
DpnI GATC 3 cut(s) 13, 225, 315
DpnII GATC 3 cut(s) 11, 223, 313
EciI GGCGGA 1 cut(s) 359
Ecl136II GAGCTC 1 cut(s) 118
Eco24I GRGCYC 2 cut(s) 120, 258
Eco47I GGWCC 1 cut(s) 50
Eco53kI GAGCTC 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 118
EcoRII CCWGG 3 cut(s) 29, 52, 265
EcoT22I ATGCAT 1 cut(s) 535
EcoT38I GRGCYC 2 cut(s) 120, 258
FaeI CATG 3 cut(s) 38, 310, 523
FaiI YATR 9 cut(s) 36, 114, 140, 189, 308, 349, 375, 521, 531
FatI CATG 3 cut(s) 34, 306, 519
FauI CCCGC 1 cut(s) 186
FbaI TGATCA 1 cut(s) 313
Fnu4HI GCNGC 3 cut(s) 21, 100, 495
FokI GGATG 4 cut(s) 12, 182, 245, 341
FriOI GRGCYC 2 cut(s) 120, 258
Fsp4HI GCNGC 3 cut(s) 21, 100, 495
FspBI CTAG 1 cut(s) 239
GlaI GCGC 1 cut(s) 289
GluI GCNGC 3 cut(s) 21, 100, 495
HaeIII GGCC 2 cut(s) 135, 506
HapII CCGG 1 cut(s) 485
HhaI GCGC 1 cut(s) 290
Hin1II CATG 3 cut(s) 38, 310, 523
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HpaII CCGG 1 cut(s) 485
HphI GGTGA 1 cut(s) 332
Hpy166II GTNNAC 3 cut(s) 59, 148, 175
Hpy188I TCNGA 1 cut(s) 512
Hpy8I GTNNAC 3 cut(s) 59, 148, 175
Hpy99I CGWCG 1 cut(s) 426
HpyAV CCTTC 1 cut(s) 287
HpyCH4III ACNGT 2 cut(s) 77, 152
HpyCH4V TGCA 5 cut(s) 59, 250, 344, 413, 533
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 350, 494, 503
HpyF3I CTNAG 3 cut(s) 40, 363, 489
Hsp92II CATG 3 cut(s) 38, 310, 523
HspAI GCGC 1 cut(s) 288
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 3 cut(s) 11, 223, 313
LmnI GCTCC 2 cut(s) 253, 391
Lsp1109I GCAGC 3 cut(s) 7, 111, 481
LweI GCATC 2 cut(s) 237, 542
MabI ACCWGGT 2 cut(s) 52, 265
MaeI CTAG 1 cut(s) 239
MalI GATC 3 cut(s) 13, 225, 315
MboI GATC 3 cut(s) 11, 223, 313
MboII GAAGA 1 cut(s) 485
MflI RGATCY 1 cut(s) 11
MhlI GDGCHC 4 cut(s) 61, 120, 258, 286
MluCI AATT 2 cut(s) 87, 417
MnlI CCTC 8 cut(s) 208, 395, 408, 427, 460, 484, 496, 549
Mph1103I ATGCAT 1 cut(s) 535
MroXI GAANNNNTTC 1 cut(s) 477
MseI TTAA 2 cut(s) 108, 299
MslI CAYNNNNRTG 1 cut(s) 186
MspI CCGG 1 cut(s) 485
MspR9I CCNGG 3 cut(s) 31, 54, 267
MvaI CCWGG 3 cut(s) 31, 54, 267
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 4 cut(s) 99, 350, 494, 503
NdeII GATC 3 cut(s) 11, 223, 313
NlaIII CATG 3 cut(s) 38, 310, 523
NlaIV GGNNCC 1 cut(s) 255
NsiI ATGCAT 1 cut(s) 535
PdmI GAANNNNTTC 1 cut(s) 477
PkrI GCNGC 3 cut(s) 22, 101, 496
PshBI ATTAAT 1 cut(s) 299
Psp124BI GAGCTC 1 cut(s) 120
Psp6I CCWGG 3 cut(s) 29, 52, 265
PspGI CCWGG 3 cut(s) 29, 52, 265
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 1 cut(s) 50
PsuI RGATCY 1 cut(s) 11
RsaI GTAC 1 cut(s) 243
RsaNI GTAC 1 cut(s) 242
RseI CAYNNNNRTG 1 cut(s) 186
SacI GAGCTC 1 cut(s) 120
SaqAI TTAA 2 cut(s) 108, 299
SatI GCNGC 3 cut(s) 21, 100, 495
Sau3AI GATC 3 cut(s) 11, 223, 313
Sau96I GGNCC 1 cut(s) 50
ScaI AGTACT 1 cut(s) 243
ScrFI CCNGG 3 cut(s) 31, 54, 267
SduI GDGCHC 4 cut(s) 61, 120, 258, 286
SexAI ACCWGGT 2 cut(s) 52, 265
SfaNI GCATC 2 cut(s) 237, 542
SinI GGWCC 1 cut(s) 50
SmiMI CAYNNNNRTG 1 cut(s) 186
SmlI CTYRAG 2 cut(s) 386, 553
SmoI CTYRAG 2 cut(s) 386, 553
Sse9I AATT 2 cut(s) 87, 417
SsiI CCGC 2 cut(s) 179, 370
SspMI CTAG 1 cut(s) 239
SstI GAGCTC 1 cut(s) 120
StyD4I CCNGG 3 cut(s) 29, 52, 265
TaaI ACNGT 2 cut(s) 77, 152
TaqI TCGA 2 cut(s) 120, 501
TasI AATT 2 cut(s) 87, 417
TatI WGTACW 1 cut(s) 241
Tru1I TTAA 2 cut(s) 108, 299
Tru9I TTAA 2 cut(s) 108, 299
TscAI CASTG 1 cut(s) 385
TseI GCWGC 3 cut(s) 20, 99, 494
TspDTI ATGAA 2 cut(s) 184, 536
TspRI CASTG 1 cut(s) 385
VneI GTGCAC 1 cut(s) 57
VpaK11BI GGWCC 1 cut(s) 50
VspI ATTAAT 1 cut(s) 299
XapI RAATTY 1 cut(s) 417
XmnI GAANNNNTTC 1 cut(s) 477
XspI CTAG 1 cut(s) 239
ZrmI AGTACT 1 cut(s) 243
Zsp2I ATGCAT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.