Rroxscaffold_2G00141630

Soluble inorganic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016505
Physical Location & Seq
Forward (+)
79848071 .. 79851832
3762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00141630.1

Sequence Viewer

Length: 651 bp
ATGGCCAACAGTGGTGGAGAAGGGAGTGGAAAGAATTCAGGATCCTCATATCAAAATGTTGCCCTGAATGAAAGGATCCTCTCATCTATGTCGCGGAGATCTGTTGCTGCTCACCCCTGGCATGACTTGGAGATCGGACCTGGTGCACCAGCAGTTTTCAACTGTGTTGTTGAAATTAGCAAAGGCAGCAAGGTCAAGTATGAGCTCGACAAAACAAGTGGCCTCATAAAAGTTGATCGTGTTCTTTATTCATCGGTTGTTTATCCACACAACTATGGTTTCATCCCGCGAACAATTTGTGAGGATAGTGATCCTATGGATGTCCTGGTACTGATGCAGGAGCCCGTGTTACCTGGTTCTTTTGTACGTGCTCGTGCTCTTGGATTGATGCCCATGATTGATCAAGGTGAAAAGGATGACAAAATTATTGCAGTATGTGCCGATGACCCTGAGTTCCGTCATTACAATGACATCAAGGACCTTCCTCCACATCGCCTTGCAGAAATCCGTCGATTCTTTGAGGATTACAAAAAGAATGAGAACAAGGAAGTTGAAGTTGAAGACTTTCTACCCGCTGAGGCTGCCATTGATGCCATCAAATACTCCATGGACCTGTATGCATCTTACATTGTGGAAAGCTTGAGGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004427 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Pyrophosphatase

Protein Analysis

216

Amino Acids

24.19

Weight (kDa)

5.09

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyrophosphatase PF00719 55 - 206 1e-57 Inorganic pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18230
fragaria_vesca FvH4_1g12970 FvH4_1g12970
malus_domestica MD02G1144200.v1.1
prunus_persica Prupe.7G157400_v2.0.a1
pyrus_communis pycom02g11410
rosa_chinensis RchiOBHm_Chr2g0101021
rosa_laevigata RLG00000017038
rosa_roxburghii Rroxscaffold_2G00141630
rosa_rugosa Rorug02G0095400
rosa_samantha Rh2BG147700 Rh2CG148900 Rh2DG148200
rosa_wichuraiana Rw2G011190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 94, 289
AciI CCGC 3 cut(s) 94, 287, 573
AclWI GGATC 5 cut(s) 36, 49, 70, 83, 305
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 34
AfaI GTAC 2 cut(s) 330, 366
AgsI TTSAA 4 cut(s) 160, 173, 554, 560
AjnI CCWGG 4 cut(s) 116, 139, 324, 352
AluBI AGCT 2 cut(s) 205, 639
AluI AGCT 2 cut(s) 205, 639
Alw21I GWGCWC 4 cut(s) 148, 207, 373, 379
Alw44I GTGCAC 1 cut(s) 144
AlwI GGATC 5 cut(s) 36, 49, 70, 83, 305
AoxI GGCC 2 cut(s) 3, 220
ApaLI GTGCAC 1 cut(s) 144
ApeKI GCWGC 3 cut(s) 107, 186, 581
ApoI RAATTY 1 cut(s) 34
Asp700I GAANNNNTTC 2 cut(s) 34, 564
AspS9I GGNCC 3 cut(s) 137, 478, 610
AsuHPI GGTGA 2 cut(s) 104, 419
AvaII GGWCC 3 cut(s) 137, 478, 610
BaeGI GKGCMC 1 cut(s) 148
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 2 cut(s) 41, 75
BanII GRGCYC 2 cut(s) 207, 345
BarI GAAGNNNNNNTAC 2 cut(s) 552, 584
BauI CACGAG 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 567
Bbv12I GWGCWC 4 cut(s) 148, 207, 373, 379
BbvCI CCTCAGC 1 cut(s) 576
BbvI GCAGC 3 cut(s) 94, 198, 568
BccI CCATC 1 cut(s) 602
BciT130I CCWGG 4 cut(s) 118, 141, 326, 354
BclI TGATCA 1 cut(s) 400
BglII AGATCT 1 cut(s) 98
BisI GCNGC 3 cut(s) 108, 187, 582
BlsI GCNGC 3 cut(s) 109, 188, 583
Bme1390I CCNGG 4 cut(s) 118, 141, 326, 354
Bme18I GGWCC 3 cut(s) 137, 478, 610
BmgT120I GGNCC 3 cut(s) 137, 478, 610
BmiI GGNNCC 3 cut(s) 43, 77, 342
BmrFI CCNGG 4 cut(s) 118, 141, 326, 354
BmsI GCATC 4 cut(s) 324, 378, 580, 629
BpiI GAAGAC 1 cut(s) 567
Bpu10I CCTNAGC 1 cut(s) 576
BsaAI YACGTR 1 cut(s) 368
BsaBI GATNNNNATC 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 116, 606
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bse8I GATNNNNATC 1 cut(s) 309
BseBI CCWGG 4 cut(s) 118, 141, 326, 354
BseDI CCNNGG 2 cut(s) 116, 606
BseGI GGATG 3 cut(s) 282, 325, 421
BseJI GATNNNNATC 1 cut(s) 309
BseMII CTCAG 2 cut(s) 441, 567
BseSI GKGCMC 1 cut(s) 148
BseXI GCAGC 3 cut(s) 94, 198, 568
Bsh1236I CGCG 2 cut(s) 94, 289
BshFI GGCC 2 cut(s) 5, 222
BsiHKAI GWGCWC 4 cut(s) 148, 207, 373, 379
BsnI GGCC 2 cut(s) 5, 222
Bsp1286I GDGCHC 5 cut(s) 148, 207, 345, 373, 379
Bsp143I GATC 7 cut(s) 41, 75, 98, 132, 235, 310, 400
Bsp19I CCATGG 1 cut(s) 606
BspACI CCGC 3 cut(s) 94, 287, 573
BspANI GGCC 2 cut(s) 5, 222
BspCNI CTCAG 2 cut(s) 442, 568
BspFNI CGCG 2 cut(s) 94, 289
BspLI GGNNCC 3 cut(s) 43, 77, 342
BspPI GGATC 5 cut(s) 36, 49, 70, 83, 305
BssECI CCNNGG 2 cut(s) 116, 606
BssMI GATC 7 cut(s) 41, 75, 98, 132, 235, 310, 400
BssSI CACGAG 1 cut(s) 372
BssT1I CCWWGG 1 cut(s) 606
Bst2BI CACGAG 1 cut(s) 372
Bst2UI CCWGG 4 cut(s) 118, 141, 326, 354
Bst4CI ACNGT 2 cut(s) 11, 164
BstAPI GCANNNNNTGC 1 cut(s) 437
BstBAI YACGTR 1 cut(s) 368
BstDEI CTNAG 2 cut(s) 450, 576
BstDSI CCRYGG 1 cut(s) 606
BstF5I GGATG 3 cut(s) 282, 325, 421
BstFNI CGCG 2 cut(s) 94, 289
BstKTI GATC 7 cut(s) 44, 78, 101, 135, 238, 313, 403
BstMBI GATC 7 cut(s) 41, 75, 98, 132, 235, 310, 400
BstMWI GCNNNNNNNGC 4 cut(s) 186, 437, 581, 590
BstNI CCWGG 4 cut(s) 118, 141, 326, 354
BstSCI CCNGG 4 cut(s) 116, 139, 324, 352
BstSLI GKGCMC 1 cut(s) 148
BstUI CGCG 2 cut(s) 94, 289
BstV1I GCAGC 3 cut(s) 94, 198, 568
BstV2I GAAGAC 1 cut(s) 567
BstX2I RGATCY 3 cut(s) 41, 75, 98
BstYI RGATCY 3 cut(s) 41, 75, 98
BsuRI GGCC 2 cut(s) 5, 222
BtgI CCRYGG 1 cut(s) 606
BtgZI GCGATG 1 cut(s) 476
BtsCI GGATG 3 cut(s) 282, 325, 421
BtsIMutI CAGTG 1 cut(s) 16
Cfr13I GGNCC 3 cut(s) 137, 478, 610
CsiI ACCWGGT 2 cut(s) 139, 352
Csp6I GTAC 2 cut(s) 329, 365
CspCI CAANNNNNGTGG 3 cut(s) 30, 199, 234
CviAII CATG 3 cut(s) 122, 394, 607
CviJI RGCY 6 cut(s) 5, 205, 222, 343, 581, 639
CviKI_1 RGCY 6 cut(s) 5, 205, 222, 343, 581, 639
CviQI GTAC 2 cut(s) 329, 365
DdeI CTNAG 2 cut(s) 450, 576
DpnI GATC 7 cut(s) 43, 77, 100, 134, 237, 312, 402
DpnII GATC 7 cut(s) 41, 75, 98, 132, 235, 310, 400
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 606
Eco24I GRGCYC 2 cut(s) 207, 345
Eco47I GGWCC 3 cut(s) 137, 478, 610
Eco53kI GAGCTC 1 cut(s) 205
EcoICRI GAGCTC 1 cut(s) 205
EcoO109I RGGNCCY 1 cut(s) 478
EcoRI GAATTC 1 cut(s) 34
EcoRII CCWGG 4 cut(s) 116, 139, 324, 352
EcoT14I CCWWGG 1 cut(s) 606
EcoT22I ATGCAT 1 cut(s) 622
EcoT38I GRGCYC 2 cut(s) 207, 345
ErhI CCWWGG 1 cut(s) 606
FaeI CATG 3 cut(s) 125, 397, 610
FatI CATG 3 cut(s) 121, 393, 606
FauI CCCGC 2 cut(s) 294, 580
FbaI TGATCA 1 cut(s) 400
Fnu4HI GCNGC 3 cut(s) 108, 187, 582
FokI GGATG 3 cut(s) 269, 332, 428
FriOI GRGCYC 2 cut(s) 207, 345
Fsp4HI GCNGC 3 cut(s) 108, 187, 582
GluI GCNGC 3 cut(s) 108, 187, 582
HaeIII GGCC 2 cut(s) 5, 222
Hin1II CATG 3 cut(s) 125, 397, 610
HindIII AAGCTT 1 cut(s) 637
HinfI GANTC 1 cut(s) 513
HphI GGTGA 2 cut(s) 104, 419
Hpy166II GTNNAC 1 cut(s) 146
Hpy188I TCNGA 1 cut(s) 137
Hpy188III TCNNGA 1 cut(s) 39
Hpy8I GTNNAC 1 cut(s) 146
Hpy99I CGWCG 1 cut(s) 513
HpyAV CCTTC 2 cut(s) 14, 491
HpyCH4III ACNGT 2 cut(s) 11, 164
HpyCH4IV ACGT 1 cut(s) 367
HpyCH4V TGCA 5 cut(s) 146, 337, 431, 500, 620
HpyF10VI GCNNNNNNNGC 4 cut(s) 186, 437, 581, 590
HpyF3I CTNAG 2 cut(s) 450, 576
HpySE526I ACGT 1 cut(s) 367
Hsp92II CATG 3 cut(s) 125, 397, 610
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 7 cut(s) 41, 75, 98, 132, 235, 310, 400
LmnI GCTCC 1 cut(s) 340
Lsp1109I GCAGC 3 cut(s) 94, 198, 568
LweI GCATC 4 cut(s) 324, 378, 580, 629
MabI ACCWGGT 2 cut(s) 139, 352
MaeII ACGT 1 cut(s) 367
MaeIII GTNAC 1 cut(s) 348
MalI GATC 7 cut(s) 43, 77, 100, 134, 237, 312, 402
MboI GATC 7 cut(s) 41, 75, 98, 132, 235, 310, 400
MboII GAAGA 1 cut(s) 572
MflI RGATCY 3 cut(s) 41, 75, 98
MhlI GDGCHC 5 cut(s) 148, 207, 345, 373, 379
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 34, 174, 294, 423
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 8 cut(s) 55, 89, 233, 295, 495, 514, 571, 636
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 622
MroXI GAANNNNTTC 2 cut(s) 34, 564
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 2 cut(s) 273, 465
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 575
MspR9I CCNGG 4 cut(s) 118, 141, 326, 354
MvaI CCWGG 4 cut(s) 118, 141, 326, 354
MvnI CGCG 2 cut(s) 94, 289
MwoI GCNNNNNNNGC 4 cut(s) 186, 437, 581, 590
NcoI CCATGG 1 cut(s) 606
NdeII GATC 7 cut(s) 41, 75, 98, 132, 235, 310, 400
NlaIII CATG 3 cut(s) 125, 397, 610
NlaIV GGNNCC 3 cut(s) 43, 77, 342
NsiI ATGCAT 1 cut(s) 622
PdmI GAANNNNTTC 2 cut(s) 34, 564
PfeI GAWTC 1 cut(s) 513
PkrI GCNGC 3 cut(s) 109, 188, 583
Ppu21I YACGTR 1 cut(s) 368
PpuMI RGGWCCY 1 cut(s) 478
Psp124BI GAGCTC 1 cut(s) 207
Psp5II RGGWCCY 1 cut(s) 478
Psp6I CCWGG 4 cut(s) 116, 139, 324, 352
PspGI CCWGG 4 cut(s) 116, 139, 324, 352
PspN4I GGNNCC 3 cut(s) 43, 77, 342
PspPI GGNCC 3 cut(s) 137, 478, 610
PspPPI RGGWCCY 1 cut(s) 478
PsuI RGATCY 3 cut(s) 41, 75, 98
RsaI GTAC 2 cut(s) 330, 366
RsaNI GTAC 2 cut(s) 329, 365
RseI CAYNNNNRTG 2 cut(s) 273, 465
SacI GAGCTC 1 cut(s) 207
SatI GCNGC 3 cut(s) 108, 187, 582
Sau3AI GATC 7 cut(s) 41, 75, 98, 132, 235, 310, 400
Sau96I GGNCC 3 cut(s) 137, 478, 610
ScrFI CCNGG 4 cut(s) 118, 141, 326, 354
SduI GDGCHC 5 cut(s) 148, 207, 345, 373, 379
SetI ASST 9 cut(s) 142, 195, 207, 355, 370, 409, 483, 615, 641
SexAI ACCWGGT 2 cut(s) 139, 352
SfaNI GCATC 4 cut(s) 324, 378, 580, 629
SinI GGWCC 3 cut(s) 137, 478, 610
SmiMI CAYNNNNRTG 2 cut(s) 273, 465
SmlI CTYRAG 1 cut(s) 640
SmoI CTYRAG 1 cut(s) 640
Sse9I AATT 4 cut(s) 34, 174, 294, 423
SsiI CCGC 3 cut(s) 94, 287, 573
SstI GAGCTC 1 cut(s) 207
StyD4I CCNGG 4 cut(s) 116, 139, 324, 352
StyI CCWWGG 1 cut(s) 606
TaaI ACNGT 2 cut(s) 11, 164
TaiI ACGT 1 cut(s) 370
TaqI TCGA 2 cut(s) 207, 511
TasI AATT 4 cut(s) 34, 174, 294, 423
TfiI GAWTC 1 cut(s) 513
TscAI CASTG 1 cut(s) 16
TseI GCWGC 3 cut(s) 107, 186, 581
TspDTI ATGAA 3 cut(s) 84, 240, 271
TspGWI ACGGA 2 cut(s) 446, 497
TspRI CASTG 1 cut(s) 16
VneI GTGCAC 1 cut(s) 144
VpaK11BI GGWCC 3 cut(s) 137, 478, 610
XapI RAATTY 1 cut(s) 34
XmnI GAANNNNTTC 2 cut(s) 34, 564
Zsp2I ATGCAT 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.