MD02G1144400.v1.1

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016238
Physical Location & Seq
Reverse (-)
11968893 .. 11972326
3434 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1144400.v1.1.491

Sequence Viewer

Length: 2256 bp
ATGGAGAATAATGGGTCAAGACACAGGAGGAATTCTCTGACGCCGGTGAAGCAAAGGAGGAGTGGATATGAGCCATCTGATGCCGAGACAGACATCCTAGATAGCCCGTGGCACGGCGGAAACAAATGGCCTAATGGAGGATATGAATCTGGAGGGGCAAAACCAGAGTTGGATTTGGGAAGAAAGATCAGCCCTTTGAGGCAGAGCAGGAGACATTCTTCCAGGATTGAATACGACGGCCCTACTCCAAGAACTACCTCAGTGGTAAGCCCAGTTCGAAAAAGAACCAACAGCAAGTCGCCCTACAAGCCTGGAAGAGGTGACAGCAATGCTCGAACTTTGCCAATGGCGGGTTCTGATATTCGTAGGAACATCAGCCCCTTGGGAAAATCCGAGCGCAGCAGACATGTTTCACCTTTTCAAGCAGACCAAGAAGAGCGCGATTTGAACAACGATGGTGATGAGATTGTAGACTCAAACAGAAACCAACATCATAAAAGAACGAACAGCAGAAGATCAGCAGCTGCTCTGCGGCATAGATCACTGAGAGAAACTGACCAGCAAAGCAATCGTTCAATCACGCCTACAAAAGGGGAGAGAACACCCTCACCATTGTCCAAAAGCATGGCTCACCAGAAGCCAGGGCAAGTGAGCACACCATCCGTCAGCGAAATCAATGAACTGCTTGCTCATGCAAAGCTTGCTAGAACTTCTCCAGATCCCATTTTGAATAACAAAGCTGCTCCGATTTACGAAACCTCAGTTTCTATTGCACCAGGTGACATCTTTTTCTCTGGAGAATGCAATGTCGTGGCATTGCCTCAAAATCACTTACAAAAACCGAAAATGGTCTCCTCTGCCGCCTCACAACAGAGAAGTACTACTCGATCAAATGGCAACTTTGGTCACGATGTCAATGTCCGCGGACTTTCATCTTCGTATGGTTTGTCACAGACAACCACAACCACTTCCAGCTCTGCGGTGAGCAGGCAGAGCAGCGGGAGGCTGAGCATGGCTAGCAGCATGAGGAGTGATGCAAGCAGGACAACAACCGCCAGCATGAGGAAGTTCACGGATAATAGGAGGAAGAGCGAAAGTCAGGCATGGTTTTCGTGTATGAGAAAGAGTTCTTGCAAGAACAAAAAATTATCACCTGAGCGTAGCACGTTCAATGAGGCTGCATTCATCGAGAAGGCGTTCGTGGAAGAGAGTCTCAGGCAGTTGTGGGCTGATAAGCATAGACCTACTTCGTTGAATGGATTCACTTGCCACAAGCAAGAAGCTCAAGTTCTCAAGCAACTTGTGATTGATAACGTCAGTCCACATATTTTGCTTACCGGACCAACTGGTTCTGGGAAGAAAGCTCTAACAATGGCGTATCTACGAGAAGTTTACGGTGATGCAAGTTGGGAAATATCTCATGATTTGAGATACTTCCAGGTTCAGGAACAAAGGACAATGCAACTGGTGGTTCCATTGACATCCAGCCCTCACCATGTGGAGCTCAATGTACAGTTGGAACCAAATGCCAGATATGCCCTAATGGGTTTGGTCAGAGAAATAAACAATGACAATGAACTTGCTCCTGAAGTCAGCTCTATAAATTTCAAGGCAAATCATAAAGAGCACATTCAGCACTTGATCAAATGGATCATGGACTGTTACTCGGATGCGTGTAAGCTCATACTTTGCTGCGAAAATGACTCCGGTGTCATTGAATCGGTGAAAAACCGGTGCAAAGTCATCAAGGTTGATGCTCCTGTGACTCATGAAATCATGGAAGTTCTTATTCAGATAGCCAGGAAAGAGGAATTTGACCTACCTATGAGCCTTGCCAATAAGATTGCAAATAAATCAAAGCAGAATCTGAGAAAAGCGATCATGGGTCTTGAAGCATGCAAAGCACATAACTATCCTTTTGGAGAAGAACAACCAATTCCACTTGGATGGGAAGAGGTGGTGCAAGAACTTGCTTCCGAAATCCTAGCTGATCCGTCACCTAAGAGACTATTTTTCATCCGGGGAAAGTTTCAAAAGCTTCTTATTGATTTTGTACATCCGAAACTGATTCTACTGAAGCTTGTGGAACAGTTCCTAAAGGGAGTGGATGCTAGTTCTAAGAGGGAACTCTATTACTGGCATGCTTATTATGAGAAAAGACTCCCAGCAGGAGCGAGTGCGTTACTGAAACTAGAAGGTAACATATGCAAGCCACCAAAATCCTTCATCCAATTATATAAGGAATCAATAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
DNA_pol3_delta2 NmrA

Protein Analysis

752

Amino Acids

84.31

Weight (kDa)

9.55

Isoelectric Point (pI)

64.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RCF1-5-like_lid PF21960 595 - 634 2.2e-09 RCF1/5-like, AAA+ ATPase lid domain
RFC_C PF22534 650 - 737 7e-09 Replication factor C subunit 3, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016238)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12990
malus_domestica MD02G1144400.v1.1 MD15G1258100.v1.1
prunus_persica Prupe.7G157200_v2.0.a1
pyrus_communis pycom15g22580
rosa_chinensis RchiOBHm_Chr2g0101071
rosa_laevigata RLG00000017049
rosa_multiflora Rmu_sc0007698.1_g000014
rosa_roxburghii Rroxscaffold_2G00141580
rosa_rugosa Rorug02G0096900
rosa_samantha Rh2AG142900 Rh2BG148000 Rh2CG149500 Rh2DG148500
rosa_wichuraiana Rw2G011260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1709
AccI GTMKAC 1 cut(s) 471
AccII CGCG 2 cut(s) 441, 924
AciI CCGC 9 cut(s) 117, 350, 532, 861, 922, 924, 980, 999, 1053
AclWI GGATC 3 cut(s) 713, 1658, 1985
AcsI RAATTY 3 cut(s) 31, 1603, 1811
AcuI CTGAAG 2 cut(s) 1608, 2096
AcyI GRCGYC 1 cut(s) 41
AdeI CACNNNGTG 2 cut(s) 779, 1498
AfaI GTAC 3 cut(s) 880, 1512, 2055
AfiI CCNNNNNNNGG 7 cut(s) 113, 137, 317, 350, 590, 1062, 1444
AflIII ACRYGT 1 cut(s) 406
AgeI ACCGGT 1 cut(s) 1731
AjnI CCWGG 6 cut(s) 221, 310, 640, 775, 1437, 1799
AjuI GAANNNNNNNTTGG 2 cut(s) 337, 369
Alw21I GWGCWC 3 cut(s) 656, 1506, 1629
Alw26I GTCTC 5 cut(s) 80, 205, 856, 1217, 1999
AlwI GGATC 3 cut(s) 713, 1658, 1985
AlwNI CAGNNNCTG 2 cut(s) 524, 1867
AoxI GGCC 2 cut(s) 128, 238
ApeKI GCWGC 8 cut(s) 399, 521, 524, 740, 996, 1020, 1178, 1692
ApoI RAATTY 3 cut(s) 31, 1603, 1811
AsiGI ACCGGT 1 cut(s) 1731
Asp700I GAANNNNTTC 3 cut(s) 1126, 1196, 1259
AspLEI GCGC 2 cut(s) 399, 441
AspS9I GGNCC 2 cut(s) 239, 1340
AsuC2I CCSGG 1 cut(s) 2021
AsuII TTCGAA 1 cut(s) 277
AsuNHI GCTAGC 1 cut(s) 1016
AvaII GGWCC 1 cut(s) 1340
BanII GRGCYC 1 cut(s) 1506
Bbv12I GWGCWC 3 cut(s) 656, 1506, 1629
BbvI GCAGC 8 cut(s) 411, 511, 533, 727, 1008, 1032, 1165, 1679
BccI CCATC 4 cut(s) 82, 449, 667, 1941
BceAI ACGGC 2 cut(s) 130, 253
BcgI CGANNNNNNTGC 4 cut(s) 551, 585, 1092, 1126
BciT130I CCWGG 6 cut(s) 223, 312, 642, 777, 1439, 1801
BclI TGATCA 1 cut(s) 1641
BcnI CCSGG 1 cut(s) 2021
BcoDI GTCTC 5 cut(s) 80, 205, 856, 1217, 1999
BfaI CTAG 6 cut(s) 98, 705, 1017, 1985, 2112, 2192
BlpI GCTNAGC 1 cut(s) 1007
BmcAI AGTACT 1 cut(s) 880
Bme1390I CCNGG 7 cut(s) 223, 312, 642, 777, 1439, 1801, 2021
Bme18I GGWCC 1 cut(s) 1340
BmgT120I GGNCC 2 cut(s) 239, 1340
BmiI GGNNCC 2 cut(s) 1473, 1521
BmrFI CCNGG 7 cut(s) 223, 312, 642, 777, 1439, 1801, 2021
BmrI ACTGGG 1 cut(s) 266
BmsI GCATC 6 cut(s) 70, 1024, 1390, 1660, 1744, 2098
BmtI GCTAGC 1 cut(s) 1020
BmuI ACTGGG 1 cut(s) 266
BplI GAGNNNNNCTC 2 cut(s) 19, 51
BpmI CTGGAG 3 cut(s) 171, 699, 816
Bpu10I CCTNAGC 1 cut(s) 1155
Bpu1102I GCTNAGC 1 cut(s) 1007
Bpu14I TTCGAA 1 cut(s) 277
BpuEI CTTGAG 2 cut(s) 1269, 1277
BpuMI CCSGG 1 cut(s) 2021
BsaBI GATNNNNATC 1 cut(s) 145
BsaHI GRCGYC 1 cut(s) 41
BsaI GGTCTC 1 cut(s) 856
BsaJI CCNNGG 5 cut(s) 107, 381, 641, 922, 2020
BsaWI WCCGGW 3 cut(s) 1337, 1706, 1731
Bsc4I CCNNNNNNNGG 7 cut(s) 113, 137, 317, 350, 590, 1062, 1444
Bse118I RCCGGY 2 cut(s) 43, 1731
Bse1I ACTGG 4 cut(s) 272, 1351, 1470, 2141
Bse3DI GCAATG 3 cut(s) 334, 811, 815
Bse8I GATNNNNATC 1 cut(s) 145
BseBI CCWGG 6 cut(s) 223, 312, 642, 777, 1439, 1801
BseDI CCNNGG 5 cut(s) 107, 381, 641, 922, 2020
BseGI GGATG 9 cut(s) 93, 659, 1481, 1675, 1952, 2016, 2056, 2113, 2226
BseJI GATNNNNATC 1 cut(s) 145
BseLI CCNNNNNNNGG 7 cut(s) 113, 137, 317, 350, 590, 1062, 1444
BseMI GCAATG 3 cut(s) 334, 811, 815
BseMII CTCAG 7 cut(s) 273, 536, 774, 998, 1146, 1228, 1859
BseNI ACTGG 4 cut(s) 272, 1351, 1470, 2141
BseRI GAGGAG 3 cut(s) 73, 844, 1042
BseXI GCAGC 8 cut(s) 411, 511, 533, 727, 1008, 1032, 1165, 1679
BseYI CCCAGC 1 cut(s) 2164
Bsh1236I CGCG 2 cut(s) 441, 924
BshFI GGCC 2 cut(s) 130, 240
BshTI ACCGGT 1 cut(s) 1731
BsiHKAI GWGCWC 3 cut(s) 656, 1506, 1629
BsiSI CCGG 5 cut(s) 44, 1338, 1707, 1732, 2020
BslI CCNNNNNNNGG 7 cut(s) 113, 137, 317, 350, 590, 1062, 1444
BsmAI GTCTC 5 cut(s) 80, 205, 856, 1217, 1999
BsmI GAATGC 2 cut(s) 806, 1181
BsnI GGCC 2 cut(s) 130, 240
Bso31I GGTCTC 1 cut(s) 856
Bsp119I TTCGAA 1 cut(s) 277
Bsp1286I GDGCHC 3 cut(s) 656, 1506, 1629
Bsp1407I TGTACA 2 cut(s) 1510, 2053
Bsp143I GATC 9 cut(s) 186, 515, 539, 718, 887, 1641, 1650, 1878, 1990
Bsp1720I GCTNAGC 1 cut(s) 1007
BspACI CCGC 9 cut(s) 117, 350, 532, 861, 922, 924, 980, 999, 1053
BspANI GGCC 2 cut(s) 130, 240
BspCNI CTCAG 7 cut(s) 272, 537, 773, 999, 1147, 1227, 1860
BspFNI CGCG 2 cut(s) 441, 924
BspHI TCATGA 2 cut(s) 1420, 1768
BspLI GGNNCC 2 cut(s) 1473, 1521
BspOI GCTAGC 1 cut(s) 1020
BspPI GGATC 3 cut(s) 713, 1658, 1985
BspQI GCTCTTC 2 cut(s) 429, 1082
BspT104I TTCGAA 1 cut(s) 277
BspTNI GGTCTC 1 cut(s) 856
BsrDI GCAATG 3 cut(s) 334, 811, 815
BsrFI RCCGGY 2 cut(s) 43, 1731
BsrGI TGTACA 2 cut(s) 1510, 2053
BsrI ACTGG 4 cut(s) 272, 1351, 1470, 2141
BssAI RCCGGY 2 cut(s) 43, 1731
BssECI CCNNGG 5 cut(s) 107, 381, 641, 922, 2020
BssMI GATC 9 cut(s) 186, 515, 539, 718, 887, 1641, 1650, 1878, 1990
BssNI GRCGYC 1 cut(s) 41
BssT1I CCWWGG 1 cut(s) 381
Bst2UI CCWGG 6 cut(s) 223, 312, 642, 777, 1439, 1801
Bst4CI ACNGT 4 cut(s) 1397, 1515, 1661, 2091
Bst6I CTCTTC 5 cut(s) 310, 429, 1082, 1200, 1947
BstACI GRCGYC 1 cut(s) 41
BstAPI GCANNNNNTGC 1 cut(s) 701
BstAUI TGTACA 2 cut(s) 1510, 2053
BstBI TTCGAA 1 cut(s) 277
BstC8I GCNNGC 9 cut(s) 687, 702, 989, 1018, 1039, 1057, 1897, 2142, 2210
BstDEI CTNAG 9 cut(s) 259, 545, 760, 1007, 1155, 1214, 1868, 2001, 2118
BstDSI CCRYGG 2 cut(s) 107, 922
BstENI CCTNNNNNAGG 3 cut(s) 135, 315, 588
BstF5I GGATG 9 cut(s) 93, 659, 1481, 1675, 1952, 2016, 2056, 2113, 2226
BstFNI CGCG 2 cut(s) 441, 924
BstHHI GCGC 2 cut(s) 399, 441
BstKTI GATC 9 cut(s) 189, 518, 542, 721, 890, 1644, 1653, 1881, 1993
BstMAI GTCTC 5 cut(s) 80, 205, 856, 1217, 1999
BstMBI GATC 9 cut(s) 186, 515, 539, 718, 887, 1641, 1650, 1878, 1990
BstMWI GCNNNNNNNGC 8 cut(s) 49, 307, 701, 993, 1017, 1535, 1633, 1901
BstNI CCWGG 6 cut(s) 223, 312, 642, 777, 1439, 1801
BstNSI RCATGY 3 cut(s) 410, 1899, 2144
BstSCI CCNGG 7 cut(s) 221, 310, 640, 775, 1437, 1799, 2019
BstUI CGCG 2 cut(s) 441, 924
BstV1I GCAGC 8 cut(s) 411, 511, 533, 727, 1008, 1032, 1165, 1679
BstX2I RGATCY 1 cut(s) 718
BstXI CCANNNNNNTGG 2 cut(s) 625, 1947
BstYI RGATCY 1 cut(s) 718
BsuRI GGCC 2 cut(s) 130, 240
BtgI CCRYGG 2 cut(s) 107, 922
BtsCI GGATG 9 cut(s) 93, 659, 1481, 1675, 1952, 2016, 2056, 2113, 2226
BtsIMutI CAGTG 2 cut(s) 267, 542
Cac8I GCNNGC 9 cut(s) 687, 702, 989, 1018, 1039, 1057, 1897, 2142, 2210
CaiI CAGNNNCTG 2 cut(s) 524, 1867
CciI TCATGA 2 cut(s) 1420, 1768
CfoI GCGC 2 cut(s) 399, 441
Cfr10I RCCGGY 2 cut(s) 43, 1731
Cfr13I GGNCC 2 cut(s) 239, 1340
Cfr42I CCGCGG 1 cut(s) 925
CseI GACGC 1 cut(s) 49
CsiI ACCWGGT 1 cut(s) 775
Csp6I GTAC 3 cut(s) 879, 1511, 2054
CspAI ACCGGT 1 cut(s) 1731
CspCI CAANNNNNGTGG 2 cut(s) 1311, 1346
CviQI GTAC 3 cut(s) 879, 1511, 2054
DdeI CTNAG 9 cut(s) 259, 545, 760, 1007, 1155, 1214, 1868, 2001, 2118
DpnI GATC 9 cut(s) 188, 517, 541, 720, 889, 1643, 1652, 1880, 1992
DpnII GATC 9 cut(s) 186, 515, 539, 718, 887, 1641, 1650, 1878, 1990
DraIII CACNNNGTG 2 cut(s) 779, 1498
DrdI GACNNNNNNGTC 1 cut(s) 1709
DseDI GACNNNNNNGTC 1 cut(s) 1709
Eam1104I CTCTTC 5 cut(s) 310, 429, 1082, 1200, 1947
EarI CTCTTC 5 cut(s) 310, 429, 1082, 1200, 1947
EciI GGCGGA 1 cut(s) 132
Ecl136II GAGCTC 1 cut(s) 1504
Eco130I CCWWGG 1 cut(s) 381
Eco24I GRGCYC 1 cut(s) 1506
Eco31I GGTCTC 1 cut(s) 856
Eco47I GGWCC 1 cut(s) 1340
Eco53kI GAGCTC 1 cut(s) 1504
Eco57I CTGAAG 2 cut(s) 1608, 2096
EcoICRI GAGCTC 1 cut(s) 1504
EcoNI CCTNNNNNAGG 3 cut(s) 135, 315, 588
EcoRI GAATTC 1 cut(s) 31
EcoRII CCWGG 6 cut(s) 221, 310, 640, 775, 1437, 1799
EcoT14I CCWWGG 1 cut(s) 381
EcoT38I GRGCYC 1 cut(s) 1506
ErhI CCWWGG 1 cut(s) 381
FauI CCCGC 2 cut(s) 343, 992
FauNDI CATATG 1 cut(s) 2204
FbaI TGATCA 1 cut(s) 1641
FblI GTMKAC 1 cut(s) 471
FokI GGATG 9 cut(s) 80, 646, 1468, 1682, 1959, 2003, 2043, 2120, 2213
FriOI GRGCYC 1 cut(s) 1506
FspBI CTAG 6 cut(s) 98, 705, 1017, 1985, 2112, 2192
GlaI GCGC 2 cut(s) 398, 440
GsaI CCCAGC 1 cut(s) 2168
GsuI CTGGAG 3 cut(s) 171, 699, 816
HaeIII GGCC 2 cut(s) 130, 240
HapII CCGG 5 cut(s) 44, 1338, 1707, 1732, 2020
HgaI GACGC 1 cut(s) 49
HhaI GCGC 2 cut(s) 399, 441
Hin1I GRCGYC 1 cut(s) 41
Hin6I GCGC 2 cut(s) 397, 439
HinP1I GCGC 2 cut(s) 397, 439
HindIII AAGCTT 3 cut(s) 698, 2036, 2078
HpaII CCGG 5 cut(s) 44, 1338, 1707, 1732, 2020
Hpy166II GTNNAC 4 cut(s) 472, 1071, 1322, 1393
Hpy8I GTNNAC 4 cut(s) 472, 1071, 1322, 1393
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 3 cut(s) 1186, 2189, 2233
HpyCH4III ACNGT 4 cut(s) 1397, 1515, 1661, 2091
HpyCH4IV ACGT 2 cut(s) 1166, 1314
HpyF10VI GCNNNNNNNGC 8 cut(s) 49, 307, 701, 993, 1017, 1535, 1633, 1901
HpyF3I CTNAG 9 cut(s) 259, 545, 760, 1007, 1155, 1214, 1868, 2001, 2118
HpySE526I ACGT 2 cut(s) 1166, 1314
Hsp92I GRCGYC 1 cut(s) 41
HspAI GCGC 2 cut(s) 397, 439
Ksp22I TGATCA 1 cut(s) 1641
KspI CCGCGG 1 cut(s) 925
Kzo9I GATC 9 cut(s) 186, 515, 539, 718, 887, 1641, 1650, 1878, 1990
LguI GCTCTTC 2 cut(s) 429, 1082
LmnI GCTCC 5 cut(s) 748, 1501, 1588, 1762, 2171
Lsp1109I GCAGC 8 cut(s) 411, 511, 533, 727, 1008, 1032, 1165, 1679
LweI GCATC 6 cut(s) 70, 1024, 1390, 1660, 1744, 2098
MabI ACCWGGT 1 cut(s) 775
MaeI CTAG 6 cut(s) 98, 705, 1017, 1985, 2112, 2192
MaeII ACGT 2 cut(s) 1166, 1314
MaeIII GTNAC 9 cut(s) 320, 779, 905, 948, 1661, 1762, 1995, 2181, 2198
MalI GATC 9 cut(s) 188, 517, 541, 720, 889, 1643, 1652, 1880, 1992
MboI GATC 9 cut(s) 186, 515, 539, 718, 887, 1641, 1650, 1878, 1990
MflI RGATCY 1 cut(s) 718
MhlI GDGCHC 3 cut(s) 656, 1506, 1629
MluCI AATT 7 cut(s) 31, 1145, 1603, 1811, 1935, 2231, 2250
MlyI GAGTC 5 cut(s) 467, 1219, 1697, 1759, 2154
MmeI TCCRAC 2 cut(s) 150, 1497
MroXI GAANNNNTTC 3 cut(s) 1126, 1196, 1259
MslI CAYNNNNRTG 1 cut(s) 1945
MspA1I CMGCKG 3 cut(s) 524, 924, 999
MspI CCGG 5 cut(s) 44, 1338, 1707, 1732, 2020
MspR9I CCNGG 7 cut(s) 223, 312, 642, 777, 1439, 1801, 2021
Mva1269I GAATGC 2 cut(s) 806, 1181
MvaI CCWGG 6 cut(s) 223, 312, 642, 777, 1439, 1801
MvnI CGCG 2 cut(s) 441, 924
MwoI GCNNNNNNNGC 8 cut(s) 49, 307, 701, 993, 1017, 1535, 1633, 1901
NciI CCSGG 1 cut(s) 2021
NdeI CATATG 1 cut(s) 2204
NdeII GATC 9 cut(s) 186, 515, 539, 718, 887, 1641, 1650, 1878, 1990
NheI GCTAGC 1 cut(s) 1016
NlaIV GGNNCC 2 cut(s) 1473, 1521
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 6 cut(s) 320, 779, 905, 948, 1762, 1995
NspI RCATGY 3 cut(s) 410, 1899, 2144
NspV TTCGAA 1 cut(s) 277
PaeI GCATGC 2 cut(s) 1899, 2144
PagI TCATGA 2 cut(s) 1420, 1768
PciI ACATGT 1 cut(s) 406
PciSI GCTCTTC 2 cut(s) 429, 1082
PctI GAATGC 2 cut(s) 806, 1181
PdmI GAANNNNTTC 3 cut(s) 1126, 1196, 1259
PfeI GAWTC 6 cut(s) 146, 1260, 1718, 1864, 2068, 2243
PfoI TCCNGGA 1 cut(s) 221
PinAI ACCGGT 1 cut(s) 1731
PleI GAGTC 5 cut(s) 467, 1218, 1697, 1759, 2154
PpsI GAGTC 5 cut(s) 467, 1218, 1697, 1759, 2154
PscI ACATGT 1 cut(s) 406
Psp124BI GAGCTC 1 cut(s) 1506
Psp6I CCWGG 6 cut(s) 221, 310, 640, 775, 1437, 1799
PspFI CCCAGC 1 cut(s) 2164
PspGI CCWGG 6 cut(s) 221, 310, 640, 775, 1437, 1799
PspN4I GGNNCC 2 cut(s) 1473, 1521
PspPI GGNCC 2 cut(s) 239, 1340
PsrI GAACNNNNNNTAC 2 cut(s) 258, 290
PstNI CAGNNNCTG 2 cut(s) 524, 1867
PsuI RGATCY 1 cut(s) 718
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 3 cut(s) 880, 1512, 2055
RsaNI GTAC 3 cut(s) 879, 1511, 2054
RseI CAYNNNNRTG 1 cut(s) 1945
SacI GAGCTC 1 cut(s) 1506
SacII CCGCGG 1 cut(s) 925
SapI GCTCTTC 2 cut(s) 429, 1082
Sau3AI GATC 9 cut(s) 186, 515, 539, 718, 887, 1641, 1650, 1878, 1990
Sau96I GGNCC 2 cut(s) 239, 1340
ScaI AGTACT 1 cut(s) 880
SchI GAGTC 5 cut(s) 467, 1219, 1697, 1759, 2154
ScrFI CCNGG 7 cut(s) 223, 312, 642, 777, 1439, 1801, 2021
SduI GDGCHC 3 cut(s) 656, 1506, 1629
SexAI ACCWGGT 1 cut(s) 775
SfaNI GCATC 6 cut(s) 70, 1024, 1390, 1660, 1744, 2098
Sfr303I CCGCGG 1 cut(s) 925
SfuI TTCGAA 1 cut(s) 277
SgrAI CRCCGGYG 1 cut(s) 43
SgrBI CCGCGG 1 cut(s) 925
SinI GGWCC 1 cut(s) 1340
SmiMI CAYNNNNRTG 1 cut(s) 1945
SmlI CTYRAG 2 cut(s) 1284, 1292
SmoI CTYRAG 2 cut(s) 1284, 1292
SphI GCATGC 2 cut(s) 1899, 2144
Sse9I AATT 7 cut(s) 31, 1145, 1603, 1811, 1935, 2231, 2250
SsiI CCGC 9 cut(s) 117, 350, 532, 861, 922, 924, 980, 999, 1053
SspMI CTAG 6 cut(s) 98, 705, 1017, 1985, 2112, 2192
SstI GAGCTC 1 cut(s) 1506
StyD4I CCNGG 7 cut(s) 221, 310, 640, 775, 1437, 1799, 2019
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 4 cut(s) 1397, 1515, 1661, 2091
TaiI ACGT 2 cut(s) 1169, 1317
TaqI TCGA 4 cut(s) 277, 334, 886, 1188
TasI AATT 7 cut(s) 31, 1145, 1603, 1811, 1935, 2231, 2250
TatI WGTACW 3 cut(s) 878, 1510, 2053
TauI GCSGC 2 cut(s) 535, 863
TfiI GAWTC 6 cut(s) 146, 1260, 1718, 1864, 2068, 2243
TscAI CASTG 2 cut(s) 267, 549
TseFI GTSAC 6 cut(s) 320, 779, 905, 948, 1762, 1995
TseI GCWGC 8 cut(s) 399, 521, 524, 740, 996, 1020, 1178, 1692
Tsp45I GTSAC 6 cut(s) 320, 779, 905, 948, 1762, 1995
TspDTI ATGAA 8 cut(s) 159, 693, 921, 1174, 1590, 1785, 2005, 2215
TspGWI ACGGA 3 cut(s) 652, 1088, 1983
TspRI CASTG 2 cut(s) 267, 549
VpaK11BI GGWCC 1 cut(s) 1340
XagI CCTNNNNNAGG 3 cut(s) 135, 315, 588
XapI RAATTY 3 cut(s) 31, 1603, 1811
XceI RCATGY 3 cut(s) 410, 1899, 2144
XmiI GTMKAC 1 cut(s) 471
XmnI GAANNNNTTC 3 cut(s) 1126, 1196, 1259
XspI CTAG 6 cut(s) 98, 705, 1017, 1985, 2112, 2192
ZrmI AGTACT 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.