MD02G1177900.v1.1

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004214
Physical Location & Seq
Forward (+)
15720744 .. 15725887
5144 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1177900.v1.1.491

Sequence Viewer

Length: 1584 bp
ATGGAGTCGAAGGCTACTGACCCGTCAACTCAACCTCCTGCTAAAGATTCTGGGGTTGAAAATGCTACTGCTGCTGCTGCTGCCTCTGGTCAAGATAATACTCAATATGCAAAAGCGGTATCTGGGGTTTTTCTATCATTTACAAAGGGTTTGGTTAACACGTCTCAGAGTGCAGTGAAGGCTGTGCAGGTCAAGGCGCGCCATTTGGTCTCCCAAAACAAACGAAGATACCAGGAGGGTGGTTTTGATTTGGATCTGACGTACATTACTGAGAATATAATTGCAATGGGTTTCCCAGCTGGAGATATAAGTTCCGGCCTATTTGGATATGTTGAGGGTTTTTATCGAAATCATATGGAAGAAGTCATCAGCTTTCTTGAGACTACTCACAAGGGAAAATATAAAGTGTACAATCTTTGTTCAGAGAGGTTGTATGATGCATCGCGATTTGAGGGGAAGGCTGCAAGTTTCCCATTTGATGATCATAATTGCCCCCCAATCGAACTCATAGAATCTTTCTGTCGAAGTGCTCATTCATGGTTAAAGGAGGGCATCGAAAATGTTGTGGTTGTTCATTGTAAAGCTGGTATGGCAAGGACAGGAGTAATGATTTGTAGCCTTCTCTTATTTCTTAAGTTCTTCCCTACAGCTGAGGAGGCCATGAATTACTATAACCATAAAAGGTGTATAGATGGAAAGGCTCTGGTTCTCCCAAGTCAGATTAGATACGTCAAATACTTTGAGCATATCTTAAAACACTTCAATGGAGAAACTCCTCCAGCTCGAAGGTGCATGCTCAGGGGATTTCGGCTTCACAATTGCCCTTATTGGATTAGGCCCTCCATTACAGTCTCGGATCATAAGGGAATTTTGTTCTCATCAAGGAAGCATCCAAAAACGAAAGATCTAATGCCAGAAGATTTCTGGATCAATGCACGGAAGAAAGGGTTTGTGGTTTTCGCTCTACCAGGGGAACCTGGTCTGGCTGAGTTAGTGGGGGATTTCAAAATCCAATTCCAAGATAGCCATGGAGATTTCTATTGCTGGTTAAATACAATGACGGAAAACAGAAAAGTCCTAACTGCAGATGACCTCGATGGTTATGAGAAGAGAAAATTACCTTGTCCCGGATTCCAGGTTGAAGTTGTGATGATAGACTATGATGGTGCTACTTTACCTACAAAAGCTAAAGCAGATTCTTCCGGTAGAACACCTGAGGGTAGCGCAAGTGATGCCAATGCTTCAAGTCATGGTGTTGCTGGAAATCCCAACCAAAGTAAAATCTCTCAGCGTGAAGATGGTGATGATGTATTTTCGGATAGTGATGGAGATGAGACTGAGGATAAAATGAGCAGGCAAGCGGAAGCGAATTCTAGAGCTGGACTTCCTGCATATAATCATCCATCTAACACCAGAAGAGTGGATAGCCCGCGGAGTAATTCCAGAAAACCAACCAACGACGGGGCTGAAAAATCTCACCCGGGCCACTCCCGCAAGTTGAGCTCATCTGGGGGAGATGATATTAGGGCAATGGCCGCGGATGCTTCAGTTTTCTCTTTCGGGGATGATTTTGATAGCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000910 GO:0001931 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0004721 GO:0004725 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005938 GO:0006464 GO:0006469 GO:0006470 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006935 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007015 GO:0007049 GO:0007154 GO:0007163 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008104 GO:0008150 GO:0008152 GO:0008154 GO:0009267 GO:0009605 GO:0009628 GO:0009651 GO:0009892 GO:0009987 GO:0009991 GO:0010563 GO:0010605 GO:0014065 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019220 GO:0019222 GO:0019538 GO:0019637 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0023052 GO:0030010 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030041 GO:0030258 GO:0030587 GO:0031152 GO:0031254 GO:0031257 GO:0031268 GO:0031269 GO:0031272 GO:0031279 GO:0031323 GO:0031324 GO:0031344 GO:0031399 GO:0031400 GO:0031667 GO:0031668 GO:0031669 GO:0032153 GO:0032155 GO:0032268 GO:0032269 GO:0032502 GO:0032796 GO:0032879 GO:0033036 GO:0033554 GO:0033673 GO:0034461 GO:0034485 GO:0034593 GO:0034594 GO:0034595 GO:0034613 GO:0034622 GO:0035335 GO:0035556 GO:0036051 GO:0036052 GO:0036211 GO:0040011 GO:0042221 GO:0042325 GO:0042326 GO:0042330 GO:0042578 GO:0042594 GO:0042995 GO:0043086 GO:0043170 GO:0043327 GO:0043412 GO:0043549 GO:0043933 GO:0044085 GO:0044087 GO:0044092 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0045761 GO:0045859 GO:0045936 GO:0046486 GO:0046488 GO:0046839 GO:0046856 GO:0048015 GO:0048017 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0050919 GO:0051128 GO:0051171 GO:0051172 GO:0051174 GO:0051179 GO:0051246 GO:0051248 GO:0051258 GO:0051270 GO:0051272 GO:0051285 GO:0051286 GO:0051301 GO:0051338 GO:0051339 GO:0051348 GO:0051641 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0051800 GO:0052866 GO:0060255 GO:0060491 GO:0061640 GO:0065003 GO:0065007 GO:0065009 GO:0070727 GO:0071496 GO:0071704 GO:0071840 GO:0071900 GO:0071901 GO:0071944 GO:0080090 GO:0090702 GO:0097435 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0106017 GO:0120025 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140096 GO:1901564 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
ko00562 ko01521 ko04068 ko04070 ko04071 ko04115 ko04140 ko04150 ko04151 ko04212 ko04218 ko04510 ko04931 ko05161 ko05165 ko05200 ko05206 ko05213 ko05214 ko05215 ko05218 ko05222 ko05224 ko05225 ko05230 map00562 map01521 map04068 map04070 map04071 map04115 map04140 map04150 map04151 map04212 map04218 map04510 map04931 map05161 map05165 map05200 map05206 map05213 map05214 map05215 map05218 map05222 map05224 map05225 map05230
Pfam Domains
Protein Families
DSPc

Protein Analysis

528

Amino Acids

58.32

Weight (kDa)

6.4

Isoelectric Point (pI)

49.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tc-R-P PF22785 156 - 209 2.2e-07 Polymorphic toxin system, DSP-PTPase phosphatase
PTEN2_C2 PF22918 262 - 388 2.9e-68 PTEN2, C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 178
AccII CGCG 4 cut(s) 199, 445, 1432, 1538
AciI CCGC 7 cut(s) 116, 1361, 1430, 1432, 1492, 1536, 1538
AclWI GGATC 3 cut(s) 261, 864, 935
AcoI YGGCCR 1 cut(s) 1533
AcsI RAATTY 2 cut(s) 867, 1369
AcuI CTGAAG 1 cut(s) 1530
AfaI GTAC 2 cut(s) 263, 410
AfiI CCNNNNNNNGG 2 cut(s) 1127, 1461
AflII CTTAAG 1 cut(s) 632
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 5 cut(s) 59, 763, 1006, 1142, 1245
AjiI CACGTC 1 cut(s) 162
AjnI CCWGG 4 cut(s) 231, 967, 976, 1134
AluBI AGCT 8 cut(s) 299, 372, 584, 650, 782, 1187, 1379, 1503
AluI AGCT 8 cut(s) 299, 372, 584, 650, 782, 1187, 1379, 1503
Alw21I GWGCWC 2 cut(s) 532, 1505
Alw26I GTCTC 5 cut(s) 168, 214, 374, 856, 1328
AlwI GGATC 3 cut(s) 261, 864, 935
Ama87I CYCGRG 1 cut(s) 1480
AoxI GGCC 5 cut(s) 316, 657, 836, 1483, 1533
ApeKI GCWGC 5 cut(s) 71, 74, 77, 80, 461
ApoI RAATTY 2 cut(s) 867, 1369
AscI GGCGCGCC 1 cut(s) 197
AspLEI GCGC 3 cut(s) 199, 201, 1226
AspS9I GGNCC 2 cut(s) 837, 1483
AsuC2I CCSGG 3 cut(s) 1128, 1481, 1482
AsuHPI GGTGA 2 cut(s) 1313, 1469
AvaI CYCGRG 1 cut(s) 1480
AxyI CCTNAGG 1 cut(s) 1215
BanII GRGCYC 1 cut(s) 1505
Bbv12I GWGCWC 2 cut(s) 532, 1505
BbvCI CCTCAGC 1 cut(s) 651
BbvI GCAGC 5 cut(s) 58, 61, 64, 67, 448
BccI CCATC 6 cut(s) 686, 1091, 1157, 1292, 1319, 1411
BciT130I CCWGG 4 cut(s) 233, 969, 978, 1136
BclI TGATCA 1 cut(s) 481
BcnI CCSGG 3 cut(s) 1128, 1481, 1482
BcoDI GTCTC 5 cut(s) 168, 214, 374, 856, 1328
BfaI CTAG 1 cut(s) 1374
BfmI CTRYAG 2 cut(s) 645, 1083
BfrI CTTAAG 1 cut(s) 632
BfuAI ACCTGC 1 cut(s) 178
BglII AGATCT 1 cut(s) 904
BisI GCNGC 6 cut(s) 72, 75, 78, 81, 462, 1536
BlsI GCNGC 6 cut(s) 73, 76, 79, 82, 463, 1537
Bme1390I CCNGG 7 cut(s) 233, 969, 978, 1128, 1136, 1481, 1482
BmeT110I CYCGRG 1 cut(s) 1480
BmgBI CACGTC 1 cut(s) 162
BmgT120I GGNCC 2 cut(s) 837, 1483
BmiI GGNNCC 1 cut(s) 975
BmrFI CCNGG 7 cut(s) 233, 969, 978, 1128, 1136, 1481, 1482
BmsI GCATC 6 cut(s) 427, 449, 561, 898, 1222, 1531
BpmI CTGGAG 2 cut(s) 321, 762
Bpu10I CCTNAGC 2 cut(s) 651, 797
BpuEI CTTGAG 1 cut(s) 398
BpuMI CCSGG 3 cut(s) 1128, 1481, 1482
BsaBI GATNNNNATC 1 cut(s) 252
BsaI GGTCTC 1 cut(s) 214
BsaJI CCNNGG 5 cut(s) 968, 1027, 1430, 1480, 1536
BsaWI WCCGGW 1 cut(s) 1202
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 2 cut(s) 1127, 1461
Bse21I CCTNAGG 1 cut(s) 1215
Bse3DI GCAATG 2 cut(s) 291, 1536
Bse8I GATNNNNATC 1 cut(s) 252
BseBI CCWGG 4 cut(s) 233, 969, 978, 1136
BseDI CCNNGG 5 cut(s) 968, 1027, 1430, 1480, 1536
BseGI GGATG 4 cut(s) 889, 1399, 1546, 1570
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 2 cut(s) 1127, 1461
BseMI GCAATG 2 cut(s) 291, 1536
BseMII CTCAG 8 cut(s) 179, 261, 642, 811, 978, 1206, 1301, 1329
BsePI GCGCGC 1 cut(s) 197
BseRI GAGGAG 2 cut(s) 668, 765
BseXI GCAGC 5 cut(s) 58, 61, 64, 67, 448
BseYI CCCAGC 1 cut(s) 295
BsgI GTGCAG 2 cut(s) 192, 206
Bsh1236I CGCG 4 cut(s) 199, 445, 1432, 1538
BshFI GGCC 5 cut(s) 318, 659, 838, 1485, 1535
BsiHKAI GWGCWC 2 cut(s) 532, 1505
BsiHKCI CYCGRG 1 cut(s) 1480
BsiSI CCGG 4 cut(s) 315, 1128, 1203, 1481
BslFI GGGAC 1 cut(s) 1110
BslI CCNNNNNNNGG 2 cut(s) 1127, 1461
BsmAI GTCTC 5 cut(s) 168, 214, 374, 856, 1328
BsmBI CGTCTC 1 cut(s) 168
BsmFI GGGAC 1 cut(s) 1110
BsnI GGCC 5 cut(s) 318, 659, 838, 1485, 1535
Bso31I GGTCTC 1 cut(s) 214
BsoBI CYCGRG 1 cut(s) 1480
Bsp1286I GDGCHC 2 cut(s) 532, 1505
Bsp1407I TGTACA 1 cut(s) 408
Bsp143I GATC 5 cut(s) 253, 481, 856, 904, 927
Bsp19I CCATGG 1 cut(s) 1027
Bsp68I TCGCGA 1 cut(s) 445
BspACI CCGC 7 cut(s) 116, 1361, 1430, 1432, 1492, 1536, 1538
BspANI GGCC 5 cut(s) 318, 659, 838, 1485, 1535
BspCNI CTCAG 8 cut(s) 178, 262, 643, 810, 979, 1207, 1300, 1330
BspFNI CGCG 4 cut(s) 199, 445, 1432, 1538
BspLI GGNNCC 1 cut(s) 975
BspMAI CTGCAG 1 cut(s) 1087
BspMI ACCTGC 1 cut(s) 178
BspPI GGATC 3 cut(s) 261, 864, 935
BspTI CTTAAG 1 cut(s) 632
BspTNI GGTCTC 1 cut(s) 214
BsrDI GCAATG 2 cut(s) 291, 1536
BsrGI TGTACA 1 cut(s) 408
BssECI CCNNGG 5 cut(s) 968, 1027, 1430, 1480, 1536
BssHII GCGCGC 1 cut(s) 197
BssMI GATC 5 cut(s) 253, 481, 856, 904, 927
BssT1I CCWWGG 1 cut(s) 1027
Bst2UI CCWGG 4 cut(s) 233, 969, 978, 1136
Bst4CI ACNGT 1 cut(s) 850
Bst6I CTCTTC 2 cut(s) 1103, 1411
BstAFI CTTAAG 1 cut(s) 632
BstAPI GCANNNNNTGC 1 cut(s) 1232
BstAUI TGTACA 1 cut(s) 408
BstC8I GCNNGC 5 cut(s) 199, 794, 1355, 1359, 1430
BstDEI CTNAG 8 cut(s) 165, 270, 651, 797, 987, 1215, 1287, 1338
BstDSI CCRYGG 3 cut(s) 1027, 1430, 1536
BstF5I GGATG 4 cut(s) 889, 1399, 1546, 1570
BstFNI CGCG 4 cut(s) 199, 445, 1432, 1538
BstHHI GCGC 3 cut(s) 199, 201, 1226
BstKTI GATC 5 cut(s) 256, 484, 859, 907, 930
BstMAI GTCTC 5 cut(s) 168, 214, 374, 856, 1328
BstMBI GATC 5 cut(s) 253, 481, 856, 904, 927
BstNI CCWGG 4 cut(s) 233, 969, 978, 1136
BstNSI RCATGY 1 cut(s) 796
BstSCI CCNGG 7 cut(s) 231, 967, 976, 1126, 1134, 1479, 1480
BstSFI CTRYAG 2 cut(s) 645, 1083
BstUI CGCG 4 cut(s) 199, 445, 1432, 1538
BstV1I GCAGC 5 cut(s) 58, 61, 64, 67, 448
BstX2I RGATCY 2 cut(s) 253, 904
BstXI CCANNNNNNTGG 2 cut(s) 239, 1420
BstYI RGATCY 2 cut(s) 253, 904
Bsu36I CCTNAGG 1 cut(s) 1215
BsuRI GGCC 5 cut(s) 318, 659, 838, 1485, 1535
BtgI CCRYGG 3 cut(s) 1027, 1430, 1536
BtgZI GCGATG 1 cut(s) 426
BtrI CACGTC 1 cut(s) 162
BtsCI GGATG 4 cut(s) 889, 1399, 1546, 1570
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 1 cut(s) 180
BtuMI TCGCGA 1 cut(s) 445
BveI ACCTGC 1 cut(s) 178
Cac8I GCNNGC 5 cut(s) 199, 794, 1355, 1359, 1430
CfoI GCGC 3 cut(s) 199, 201, 1226
Cfr13I GGNCC 2 cut(s) 837, 1483
Cfr42I CCGCGG 2 cut(s) 1433, 1539
Cfr9I CCCGGG 1 cut(s) 1480
CsiI ACCWGGT 1 cut(s) 976
Csp6I GTAC 2 cut(s) 262, 409
CviAII CATG 5 cut(s) 537, 661, 793, 1028, 1250
CviQI GTAC 2 cut(s) 262, 409
DdeI CTNAG 8 cut(s) 165, 270, 651, 797, 987, 1215, 1287, 1338
DpnI GATC 5 cut(s) 255, 483, 858, 906, 929
DpnII GATC 5 cut(s) 253, 481, 856, 904, 927
EaeI YGGCCR 1 cut(s) 1533
Eam1104I CTCTTC 2 cut(s) 1103, 1411
EarI CTCTTC 2 cut(s) 1103, 1411
Ecl136II GAGCTC 1 cut(s) 1503
Eco130I CCWWGG 1 cut(s) 1027
Eco24I GRGCYC 1 cut(s) 1505
Eco31I GGTCTC 1 cut(s) 214
Eco53kI GAGCTC 1 cut(s) 1503
Eco57I CTGAAG 1 cut(s) 1530
Eco81I CCTNAGG 1 cut(s) 1215
Eco88I CYCGRG 1 cut(s) 1480
EcoICRI GAGCTC 1 cut(s) 1503
EcoO109I RGGNCCY 1 cut(s) 837
EcoRI GAATTC 1 cut(s) 1369
EcoRII CCWGG 4 cut(s) 231, 967, 976, 1134
EcoT14I CCWWGG 1 cut(s) 1027
EcoT22I ATGCAT 1 cut(s) 442
EcoT38I GRGCYC 1 cut(s) 1505
ErhI CCWWGG 1 cut(s) 1027
Esp3I CGTCTC 1 cut(s) 168
FaeI CATG 5 cut(s) 540, 664, 796, 1031, 1253
FaqI GGGAC 1 cut(s) 1110
FatI CATG 5 cut(s) 536, 660, 792, 1027, 1249
FauI CCCGC 2 cut(s) 1437, 1499
FauNDI CATATG 1 cut(s) 354
FbaI TGATCA 1 cut(s) 481
Fnu4HI GCNGC 6 cut(s) 72, 75, 78, 81, 462, 1536
FokI GGATG 4 cut(s) 876, 1386, 1553, 1577
FriOI GRGCYC 1 cut(s) 1505
Fsp4HI GCNGC 6 cut(s) 72, 75, 78, 81, 462, 1536
FspBI CTAG 1 cut(s) 1374
GlaI GCGC 3 cut(s) 198, 200, 1225
GluI GCNGC 6 cut(s) 72, 75, 78, 81, 462, 1536
GsaI CCCAGC 1 cut(s) 299
GsuI CTGGAG 2 cut(s) 321, 762
HaeIII GGCC 5 cut(s) 318, 659, 838, 1485, 1535
HapII CCGG 4 cut(s) 315, 1128, 1203, 1481
HhaI GCGC 3 cut(s) 199, 201, 1226
Hin1II CATG 5 cut(s) 540, 664, 796, 1031, 1253
Hin6I GCGC 3 cut(s) 197, 199, 1224
HinP1I GCGC 3 cut(s) 197, 199, 1224
HincII GTYRAC 2 cut(s) 27, 157
HindII GTYRAC 2 cut(s) 27, 157
HinfI GANTC 5 cut(s) 5, 47, 512, 1131, 1196
HpaI GTTAAC 1 cut(s) 157
HpaII CCGG 4 cut(s) 315, 1128, 1203, 1481
HphI GGTGA 2 cut(s) 1313, 1469
Hpy166II GTNNAC 3 cut(s) 27, 157, 409
Hpy188I TCNGA 6 cut(s) 168, 258, 424, 720, 856, 1318
Hpy188III TCNNGA 6 cut(s) 92, 377, 444, 925, 1374, 1443
Hpy8I GTNNAC 3 cut(s) 27, 157, 409
Hpy99I CGWCG 1 cut(s) 1463
HpyAV CCTTC 5 cut(s) 4, 172, 451, 629, 780
HpyCH4III ACNGT 1 cut(s) 850
HpyCH4IV ACGT 3 cut(s) 161, 260, 729
HpyF3I CTNAG 8 cut(s) 165, 270, 651, 797, 987, 1215, 1287, 1338
HpySE526I ACGT 3 cut(s) 161, 260, 729
Hsp92II CATG 5 cut(s) 540, 664, 796, 1031, 1253
HspAI GCGC 3 cut(s) 197, 199, 1224
Ksp22I TGATCA 1 cut(s) 481
KspAI GTTAAC 1 cut(s) 157
KspI CCGCGG 2 cut(s) 1433, 1539
Kzo9I GATC 5 cut(s) 253, 481, 856, 904, 927
Lsp1109I GCAGC 5 cut(s) 58, 61, 64, 67, 448
LweI GCATC 6 cut(s) 427, 449, 561, 898, 1222, 1531
MabI ACCWGGT 1 cut(s) 976
MaeI CTAG 1 cut(s) 1374
MaeII ACGT 3 cut(s) 161, 260, 729
MalI GATC 5 cut(s) 255, 483, 858, 906, 929
MboI GATC 5 cut(s) 253, 481, 856, 904, 927
MboII GAAGA 9 cut(s) 237, 371, 631, 929, 952, 1120, 1191, 1307, 1428
MfeI CAATTG 1 cut(s) 817
MflI RGATCY 2 cut(s) 253, 904
MhlI GDGCHC 2 cut(s) 532, 1505
MluCI AATT 9 cut(s) 279, 487, 664, 817, 867, 1013, 1115, 1369, 1438
MlyI GAGTC 1 cut(s) 14
Mph1103I ATGCAT 1 cut(s) 442
MseI TTAA 5 cut(s) 156, 542, 633, 752, 1049
MslI CAYNNNNRTG 1 cut(s) 762
MspA1I CMGCKG 4 cut(s) 299, 650, 1432, 1538
MspCI CTTAAG 1 cut(s) 632
MspI CCGG 4 cut(s) 315, 1128, 1203, 1481
MspR9I CCNGG 7 cut(s) 233, 969, 978, 1128, 1136, 1481, 1482
MunI CAATTG 1 cut(s) 817
MvaI CCWGG 4 cut(s) 233, 969, 978, 1136
MvnI CGCG 4 cut(s) 199, 445, 1432, 1538
NciI CCSGG 3 cut(s) 1128, 1481, 1482
NcoI CCATGG 1 cut(s) 1027
NdeI CATATG 1 cut(s) 354
NdeII GATC 5 cut(s) 253, 481, 856, 904, 927
NlaIII CATG 5 cut(s) 540, 664, 796, 1031, 1253
NlaIV GGNNCC 1 cut(s) 975
NruI TCGCGA 1 cut(s) 445
NsiI ATGCAT 1 cut(s) 442
NspI RCATGY 1 cut(s) 796
PaeI GCATGC 1 cut(s) 796
PalAI GGCGCGCC 1 cut(s) 197
PauI GCGCGC 1 cut(s) 197
PfeI GAWTC 4 cut(s) 47, 512, 1131, 1196
PfoI TCCNGGA 1 cut(s) 1126
PkrI GCNGC 6 cut(s) 73, 76, 79, 82, 463, 1537
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 1505
Psp6I CCWGG 4 cut(s) 231, 967, 976, 1134
PspFI CCCAGC 1 cut(s) 295
PspGI CCWGG 4 cut(s) 231, 967, 976, 1134
PspN4I GGNNCC 1 cut(s) 975
PspPI GGNCC 2 cut(s) 837, 1483
PstI CTGCAG 1 cut(s) 1087
PsuI RGATCY 2 cut(s) 253, 904
PteI GCGCGC 1 cut(s) 197
PvuII CAGCTG 2 cut(s) 299, 650
RruI TCGCGA 1 cut(s) 445
RsaI GTAC 2 cut(s) 263, 410
RsaNI GTAC 2 cut(s) 262, 409
RseI CAYNNNNRTG 1 cut(s) 762
SacI GAGCTC 1 cut(s) 1505
SacII CCGCGG 2 cut(s) 1433, 1539
SaqAI TTAA 5 cut(s) 156, 542, 633, 752, 1049
SatI GCNGC 6 cut(s) 72, 75, 78, 81, 462, 1536
Sau3AI GATC 5 cut(s) 253, 481, 856, 904, 927
Sau96I GGNCC 2 cut(s) 837, 1483
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 7 cut(s) 233, 969, 978, 1128, 1136, 1481, 1482
SduI GDGCHC 2 cut(s) 532, 1505
SexAI ACCWGGT 1 cut(s) 976
SfaNI GCATC 6 cut(s) 427, 449, 561, 898, 1222, 1531
SfcI CTRYAG 2 cut(s) 645, 1083
Sfr303I CCGCGG 2 cut(s) 1433, 1539
SgrBI CCGCGG 2 cut(s) 1433, 1539
SgsI GGCGCGCC 1 cut(s) 197
SmaI CCCGGG 1 cut(s) 1482
SmiMI CAYNNNNRTG 1 cut(s) 762
SmlI CTYRAG 2 cut(s) 377, 632
SmoI CTYRAG 2 cut(s) 377, 632
SphI GCATGC 1 cut(s) 796
Sse9I AATT 9 cut(s) 279, 487, 664, 817, 867, 1013, 1115, 1369, 1438
SsiI CCGC 7 cut(s) 116, 1361, 1430, 1432, 1492, 1536, 1538
SspMI CTAG 1 cut(s) 1374
SstI GAGCTC 1 cut(s) 1505
StyD4I CCNGG 7 cut(s) 231, 967, 976, 1126, 1134, 1479, 1480
StyI CCWWGG 1 cut(s) 1027
TaaI ACNGT 1 cut(s) 850
TaiI ACGT 3 cut(s) 164, 263, 732
TaqI TCGA 7 cut(s) 8, 346, 501, 523, 555, 784, 1095
TasI AATT 9 cut(s) 279, 487, 664, 817, 867, 1013, 1115, 1369, 1438
TatI WGTACW 1 cut(s) 408
TauI GCSGC 1 cut(s) 1538
TfiI GAWTC 4 cut(s) 47, 512, 1131, 1196
Tru1I TTAA 5 cut(s) 156, 542, 633, 752, 1049
Tru9I TTAA 5 cut(s) 156, 542, 633, 752, 1049
TscAI CASTG 1 cut(s) 180
TseI GCWGC 5 cut(s) 71, 74, 77, 80, 461
TspDTI ATGAA 3 cut(s) 525, 563, 677
TspGWI ACGGA 2 cut(s) 952, 1076
TspMI CCCGGG 1 cut(s) 1480
TspRI CASTG 1 cut(s) 180
Vha464I CTTAAG 1 cut(s) 632
XapI RAATTY 2 cut(s) 867, 1369
XbaI TCTAGA 1 cut(s) 1373
XceI RCATGY 1 cut(s) 796
XcmI CCANNNNNNNNNTGG 2 cut(s) 921, 1025
XmaI CCCGGG 1 cut(s) 1480
XspI CTAG 1 cut(s) 1374
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.