MD15G1287800.v1.1

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004214
Physical Location & Seq
Forward (+)
26443020 .. 26448797
5778 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1287800.v1.1.491

Sequence Viewer

Length: 1584 bp
ATGGAGTCGAAAGCTAATGACCCGTCAACTCAACCTCCTGCTAAAGATTCTGGGGTTGAAAATGCTACTGCTGCCTCTGGTCAAGATAATAATAGCACTCAATATGCAAAAGCGGTATCTGGGGTTTTTCTTTCATTTACAAAGGGTTTGTTTAACACATCTCAGAGCGCAGTGAAGGCTGTGCAGGTCAAGGCGCGCCATTTGGTCTCTCAAAACAAACGAAGATACCAGGAGGGGGGTTTTGATTTGGATCTGACATACATTACTGAGAATATAATTGCAATGGGTTTCCCAGCTGGAGATATAAGCTCAGGTCTATTTGGATATGTTGAGGGTTTTTACCGAAACCACATGGAAGAAGTCATCAGATTTCTTGAGACTACTCACAAGGGAAAATATAAAGTGTACAATCTTTGTTCAGAGAGATTGTATGATGCATCGCGATTTGAGGGGAAGGCTGCAAGTTTCCCGTTTGATGATCATAATTGCCCCCCAATCGAACTCATAGAATCTTTCTGTCGAAGTGCTCATTCATGGTTAAAGGAGGGCATTGAAAATGTTGTGGTCGTCCATTGTAAAGCTGGTATGGCAAGGACAGGAGTAATGATTTGTAGCCTTCTCTTACTTCTTAAGTTCTTCCCTACGGCTGCGGAGGCCATGAATTACTATAACCATAAAAGATGTATAGATGGAAAGGCTTTGGTTCTCCCTAGTCAGATTAGATATGTCAAATACTTTGAGCATATCTTAAAACACTTCAATGGAGAAACTCCTCCACCTCGAAGGTGCATGCTCAGGGGATTTCGGCTTCACAATTGCCCTTATTGGATTAGGCCCTCCATTACAATCTCGGATCATAAAGGAATTTTGTTCTCATCAAGAAAGCATCCAAAAACAAAAGATCTTATGCCAGAAGATTTCTGGATCAGTGCACGGAAGAAAGGGATTGTGGTTTTCGCTCTACCAGGGGAACCTGGTCTTGCTGAGTTAGTGGGGGATTTCAAAATCCATTTCCTAGATAGCCATGGAGATTTCTATTGCTGGTTAAATACAATGACAGAAAACAGAAAAATCTTAACCGCAGATGACCTCGATGATTTTGACAAGAGAAAATTCCCTTGTCCAGGATTTCAGGTTGAAGTTGTGATGATAGACTATGATGGTACTGCTTTGCCTACAAAAGATAAAGCAGATTCTTCCGGTAGAAAACCTGAGGGTAGCTCAAGTGACGCCAATGCTTCAAGTCGTGGTGTTGCAGGAAACCCCAACCAAAGTAAAATCTTTCAGCGTGAAGATGGTGACGATGTATTTTCAGATAGTGATGGAGATGAGCCTGGGGATAAAACGAGCAGGCAACCGGAAGCTAATTCTAGAGCTGGAATTGCTGCATCCAATCATCCATCTAACACCAGAAGTGTGGATAGCCCGCGGAGAACTTCCAGAAAACCGACTAGTGATGGAGCTGAAAAATCTCACCTGGGCCACTCCCGCAAGTTGAGCTCATCGGGGGCAGATGATATAAGGGCGATAGCCGCGGATGCTTCAGTTTTCTCTTTCGGGGATGACGATTTTGATAGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000910 GO:0001931 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0004721 GO:0004725 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005938 GO:0006464 GO:0006469 GO:0006470 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006935 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007015 GO:0007049 GO:0007154 GO:0007163 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008104 GO:0008150 GO:0008152 GO:0008154 GO:0009267 GO:0009605 GO:0009628 GO:0009651 GO:0009892 GO:0009987 GO:0009991 GO:0010563 GO:0010605 GO:0014065 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019220 GO:0019222 GO:0019538 GO:0019637 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0023052 GO:0030010 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030041 GO:0030258 GO:0030587 GO:0031152 GO:0031254 GO:0031257 GO:0031268 GO:0031269 GO:0031272 GO:0031279 GO:0031323 GO:0031324 GO:0031344 GO:0031399 GO:0031400 GO:0031667 GO:0031668 GO:0031669 GO:0032153 GO:0032155 GO:0032268 GO:0032269 GO:0032502 GO:0032796 GO:0032879 GO:0033036 GO:0033554 GO:0033673 GO:0034461 GO:0034485 GO:0034593 GO:0034594 GO:0034595 GO:0034613 GO:0034622 GO:0035335 GO:0035556 GO:0036051 GO:0036052 GO:0036211 GO:0040011 GO:0042221 GO:0042325 GO:0042326 GO:0042330 GO:0042578 GO:0042594 GO:0042995 GO:0043086 GO:0043170 GO:0043327 GO:0043412 GO:0043549 GO:0043933 GO:0044085 GO:0044087 GO:0044092 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0045761 GO:0045859 GO:0045936 GO:0046486 GO:0046488 GO:0046839 GO:0046856 GO:0048015 GO:0048017 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0050919 GO:0051128 GO:0051171 GO:0051172 GO:0051174 GO:0051179 GO:0051246 GO:0051248 GO:0051258 GO:0051270 GO:0051272 GO:0051285 GO:0051286 GO:0051301 GO:0051338 GO:0051339 GO:0051348 GO:0051641 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0051800 GO:0052866 GO:0060255 GO:0060491 GO:0061640 GO:0065003 GO:0065007 GO:0065009 GO:0070727 GO:0071496 GO:0071704 GO:0071840 GO:0071900 GO:0071901 GO:0071944 GO:0080090 GO:0090702 GO:0097435 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0106017 GO:0120025 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140096 GO:1901564 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
ko00562 ko01521 ko04068 ko04070 ko04071 ko04115 ko04140 ko04150 ko04151 ko04212 ko04218 ko04510 ko04931 ko05161 ko05165 ko05200 ko05206 ko05213 ko05214 ko05215 ko05218 ko05222 ko05224 ko05225 ko05230 map00562 map01521 map04068 map04070 map04071 map04115 map04140 map04150 map04151 map04212 map04218 map04510 map04931 map05161 map05165 map05200 map05206 map05213 map05214 map05215 map05218 map05222 map05224 map05225 map05230
Pfam Domains
Protein Families
DSPc

Protein Analysis

528

Amino Acids

58.47

Weight (kDa)

6.64

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tc-R-P PF22785 155 - 208 2.4e-07 Polymorphic toxin system, DSP-PTPase phosphatase
PTEN2_C2 PF22918 261 - 387 6.6e-69 PTEN2, C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 175
AccII CGCG 4 cut(s) 196, 442, 1429, 1535
AciI CCGC 8 cut(s) 113, 650, 1080, 1427, 1429, 1489, 1533, 1535
AclWI GGATC 3 cut(s) 258, 861, 932
AcsI RAATTY 2 cut(s) 864, 1112
AcuI CTGAAG 1 cut(s) 1527
AcyI GRCGYC 1 cut(s) 1230
AfaI GTAC 2 cut(s) 407, 1165
AfiI CCNNNNNNNGG 2 cut(s) 235, 1124
AflII CTTAAG 1 cut(s) 629
AgsI TTSAA 6 cut(s) 59, 554, 760, 1003, 1139, 1242
AhlI ACTAGT 1 cut(s) 1451
AjnI CCWGG 6 cut(s) 228, 964, 973, 1123, 1333, 1476
AluBI AGCT 9 cut(s) 14, 296, 309, 581, 1221, 1364, 1376, 1463, 1500
AluI AGCT 9 cut(s) 14, 296, 309, 581, 1221, 1364, 1376, 1463, 1500
Alw21I GWGCWC 3 cut(s) 529, 934, 1502
Alw26I GTCTC 2 cut(s) 211, 371
Alw44I GTGCAC 1 cut(s) 930
AlwI GGATC 3 cut(s) 258, 861, 932
AoxI GGCC 3 cut(s) 654, 833, 1480
ApaLI GTGCAC 1 cut(s) 930
ApeKI GCWGC 4 cut(s) 71, 458, 647, 1385
ApoI RAATTY 2 cut(s) 864, 1112
AscI GGCGCGCC 1 cut(s) 194
AspLEI GCGC 3 cut(s) 170, 196, 198
AspS9I GGNCC 2 cut(s) 834, 1480
AsuHPI GGTGA 2 cut(s) 1310, 1466
AxyI CCTNAGG 1 cut(s) 1212
BaeGI GKGCMC 1 cut(s) 934
BanII GRGCYC 1 cut(s) 1502
Bbv12I GWGCWC 3 cut(s) 529, 934, 1502
BbvI GCAGC 4 cut(s) 58, 445, 634, 1372
BccI CCATC 6 cut(s) 683, 1154, 1289, 1316, 1408, 1451
BceAI ACGGC 1 cut(s) 660
BciT130I CCWGG 6 cut(s) 230, 966, 975, 1125, 1335, 1478
BclI TGATCA 1 cut(s) 478
BcoDI GTCTC 2 cut(s) 211, 371
BcuI ACTAGT 1 cut(s) 1451
BfaI CTAG 4 cut(s) 711, 1016, 1371, 1452
BfrI CTTAAG 1 cut(s) 629
BfuAI ACCTGC 1 cut(s) 175
BglII AGATCT 1 cut(s) 901
BisI GCNGC 5 cut(s) 72, 459, 648, 1386, 1533
BlsI GCNGC 5 cut(s) 73, 460, 649, 1387, 1534
Bme1390I CCNGG 6 cut(s) 230, 966, 975, 1125, 1335, 1478
BmgT120I GGNCC 2 cut(s) 834, 1480
BmiI GGNNCC 1 cut(s) 972
BmrFI CCNGG 6 cut(s) 230, 966, 975, 1125, 1335, 1478
BmsI GCATC 5 cut(s) 424, 446, 895, 1397, 1528
BplI GAGNNNNNCTC 2 cut(s) 1205, 1237
BpmI CTGGAG 1 cut(s) 318
Bpu10I CCTNAGC 2 cut(s) 310, 794
BpuEI CTTGAG 2 cut(s) 395, 1207
BsaBI GATNNNNATC 1 cut(s) 249
BsaHI GRCGYC 1 cut(s) 1230
BsaI GGTCTC 1 cut(s) 211
BsaJI CCNNGG 6 cut(s) 965, 1024, 1334, 1427, 1477, 1533
BsaWI WCCGGW 2 cut(s) 1199, 1357
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 1124
Bse21I CCTNAGG 1 cut(s) 1212
Bse3DI GCAATG 1 cut(s) 288
Bse8I GATNNNNATC 1 cut(s) 249
BseBI CCWGG 6 cut(s) 230, 966, 975, 1125, 1335, 1478
BseDI CCNNGG 6 cut(s) 965, 1024, 1334, 1427, 1477, 1533
BseGI GGATG 5 cut(s) 886, 1388, 1396, 1543, 1567
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 2 cut(s) 235, 1124
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 6 cut(s) 176, 258, 324, 808, 975, 1203
BsePI GCGCGC 1 cut(s) 194
BseRI GAGGAG 1 cut(s) 762
BseSI GKGCMC 1 cut(s) 934
BseXI GCAGC 4 cut(s) 58, 445, 634, 1372
BseYI CCCAGC 1 cut(s) 292
BsgI GTGCAG 1 cut(s) 203
Bsh1236I CGCG 4 cut(s) 196, 442, 1429, 1535
BshFI GGCC 3 cut(s) 656, 835, 1482
BsiHKAI GWGCWC 3 cut(s) 529, 934, 1502
BsiSI CCGG 2 cut(s) 1200, 1358
BslI CCNNNNNNNGG 2 cut(s) 235, 1124
BsmAI GTCTC 2 cut(s) 211, 371
BsnI GGCC 3 cut(s) 656, 835, 1482
Bso31I GGTCTC 1 cut(s) 211
Bsp1286I GDGCHC 3 cut(s) 529, 934, 1502
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 5 cut(s) 250, 478, 853, 901, 924
Bsp19I CCATGG 1 cut(s) 1024
Bsp68I TCGCGA 1 cut(s) 442
BspACI CCGC 8 cut(s) 113, 650, 1080, 1427, 1429, 1489, 1533, 1535
BspANI GGCC 3 cut(s) 656, 835, 1482
BspCNI CTCAG 6 cut(s) 175, 259, 323, 807, 976, 1204
BspFNI CGCG 4 cut(s) 196, 442, 1429, 1535
BspLI GGNNCC 1 cut(s) 972
BspMI ACCTGC 1 cut(s) 175
BspPI GGATC 3 cut(s) 258, 861, 932
BspTI CTTAAG 1 cut(s) 629
BspTNI GGTCTC 1 cut(s) 211
BsrDI GCAATG 1 cut(s) 288
BsrGI TGTACA 1 cut(s) 405
BssECI CCNNGG 6 cut(s) 965, 1024, 1334, 1427, 1477, 1533
BssHII GCGCGC 1 cut(s) 194
BssMI GATC 5 cut(s) 250, 478, 853, 901, 924
BssNI GRCGYC 1 cut(s) 1230
BssT1I CCWWGG 1 cut(s) 1024
Bst2UI CCWGG 6 cut(s) 230, 966, 975, 1125, 1335, 1478
BstACI GRCGYC 1 cut(s) 1230
BstAFI CTTAAG 1 cut(s) 629
BstAUI TGTACA 1 cut(s) 405
BstC8I GCNNGC 4 cut(s) 196, 791, 1352, 1427
BstDEI CTNAG 6 cut(s) 162, 267, 310, 794, 984, 1212
BstDSI CCRYGG 3 cut(s) 1024, 1427, 1533
BstENI CCTNNNNNAGG 1 cut(s) 1122
BstF5I GGATG 5 cut(s) 886, 1388, 1396, 1543, 1567
BstFNI CGCG 4 cut(s) 196, 442, 1429, 1535
BstHHI GCGC 3 cut(s) 170, 196, 198
BstKTI GATC 5 cut(s) 253, 481, 856, 904, 927
BstMAI GTCTC 2 cut(s) 211, 371
BstMBI GATC 5 cut(s) 250, 478, 853, 901, 924
BstMWI GCNNNNNNNGC 9 cut(s) 71, 176, 587, 653, 1382, 1488, 1497, 1532, 1538
BstNI CCWGG 6 cut(s) 230, 966, 975, 1125, 1335, 1478
BstNSI RCATGY 1 cut(s) 793
BstSCI CCNGG 6 cut(s) 228, 964, 973, 1123, 1333, 1476
BstSLI GKGCMC 1 cut(s) 934
BstUI CGCG 4 cut(s) 196, 442, 1429, 1535
BstV1I GCAGC 4 cut(s) 58, 445, 634, 1372
BstX2I RGATCY 2 cut(s) 250, 901
BstXI CCANNNNNNTGG 1 cut(s) 1417
BstYI RGATCY 2 cut(s) 250, 901
Bsu36I CCTNAGG 1 cut(s) 1212
BsuRI GGCC 3 cut(s) 656, 835, 1482
BtgI CCRYGG 3 cut(s) 1024, 1427, 1533
BtgZI GCGATG 1 cut(s) 423
BtsCI GGATG 5 cut(s) 886, 1388, 1396, 1543, 1567
BtsI GCAGTG 1 cut(s) 177
BtsIMutI CAGTG 2 cut(s) 177, 934
BtuMI TCGCGA 1 cut(s) 442
BveI ACCTGC 1 cut(s) 175
Cac8I GCNNGC 4 cut(s) 196, 791, 1352, 1427
CfoI GCGC 3 cut(s) 170, 196, 198
Cfr13I GGNCC 2 cut(s) 834, 1480
Cfr42I CCGCGG 2 cut(s) 1430, 1536
CseI GACGC 1 cut(s) 1238
CsiI ACCWGGT 1 cut(s) 973
Csp6I GTAC 2 cut(s) 406, 1164
CviAII CATG 5 cut(s) 352, 534, 658, 790, 1025
CviQI GTAC 2 cut(s) 406, 1164
DdeI CTNAG 6 cut(s) 162, 267, 310, 794, 984, 1212
DpnI GATC 5 cut(s) 252, 480, 855, 903, 926
DpnII GATC 5 cut(s) 250, 478, 853, 901, 924
Ecl136II GAGCTC 1 cut(s) 1500
Eco130I CCWWGG 1 cut(s) 1024
Eco24I GRGCYC 1 cut(s) 1502
Eco31I GGTCTC 1 cut(s) 211
Eco53kI GAGCTC 1 cut(s) 1500
Eco57I CTGAAG 1 cut(s) 1527
Eco81I CCTNAGG 1 cut(s) 1212
EcoICRI GAGCTC 1 cut(s) 1500
EcoNI CCTNNNNNAGG 1 cut(s) 1122
EcoO109I RGGNCCY 1 cut(s) 834
EcoRII CCWGG 6 cut(s) 228, 964, 973, 1123, 1333, 1476
EcoT14I CCWWGG 1 cut(s) 1024
EcoT22I ATGCAT 1 cut(s) 439
EcoT38I GRGCYC 1 cut(s) 1502
ErhI CCWWGG 1 cut(s) 1024
FaeI CATG 5 cut(s) 355, 537, 661, 793, 1028
FatI CATG 5 cut(s) 351, 533, 657, 789, 1024
FauI CCCGC 2 cut(s) 1434, 1496
FbaI TGATCA 1 cut(s) 478
Fnu4HI GCNGC 5 cut(s) 72, 459, 648, 1386, 1533
FokI GGATG 5 cut(s) 873, 1375, 1383, 1550, 1574
FriOI GRGCYC 1 cut(s) 1502
Fsp4HI GCNGC 5 cut(s) 72, 459, 648, 1386, 1533
FspBI CTAG 4 cut(s) 711, 1016, 1371, 1452
GlaI GCGC 3 cut(s) 169, 195, 197
GluI GCNGC 5 cut(s) 72, 459, 648, 1386, 1533
GsaI CCCAGC 1 cut(s) 296
GsuI CTGGAG 1 cut(s) 318
HaeIII GGCC 3 cut(s) 656, 835, 1482
HapII CCGG 2 cut(s) 1200, 1358
HgaI GACGC 1 cut(s) 1238
HhaI GCGC 3 cut(s) 170, 196, 198
Hin1I GRCGYC 1 cut(s) 1230
Hin1II CATG 5 cut(s) 355, 537, 661, 793, 1028
Hin6I GCGC 3 cut(s) 168, 194, 196
HinP1I GCGC 3 cut(s) 168, 194, 196
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 4 cut(s) 5, 47, 509, 1193
HpaII CCGG 2 cut(s) 1200, 1358
HphI GGTGA 2 cut(s) 1310, 1466
Hpy166II GTNNAC 3 cut(s) 27, 406, 932
Hpy188I TCNGA 7 cut(s) 165, 255, 368, 421, 717, 853, 1315
Hpy188III TCNNGA 7 cut(s) 83, 374, 441, 879, 922, 1371, 1440
Hpy8I GTNNAC 3 cut(s) 27, 406, 932
HpyAV CCTTC 4 cut(s) 169, 448, 626, 777
HpyCH4V TGCA 9 cut(s) 107, 184, 281, 437, 461, 789, 932, 1256, 1388
HpyF10VI GCNNNNNNNGC 9 cut(s) 71, 176, 587, 653, 1382, 1488, 1497, 1532, 1538
HpyF3I CTNAG 6 cut(s) 162, 267, 310, 794, 984, 1212
Hsp92I GRCGYC 1 cut(s) 1230
Hsp92II CATG 5 cut(s) 355, 537, 661, 793, 1028
HspAI GCGC 3 cut(s) 168, 194, 196
Ksp22I TGATCA 1 cut(s) 478
KspI CCGCGG 2 cut(s) 1430, 1536
Kzo9I GATC 5 cut(s) 250, 478, 853, 901, 924
LmnI GCTCC 1 cut(s) 1460
Lsp1109I GCAGC 4 cut(s) 58, 445, 634, 1372
LweI GCATC 5 cut(s) 424, 446, 895, 1397, 1528
MabI ACCWGGT 1 cut(s) 973
MaeI CTAG 4 cut(s) 711, 1016, 1371, 1452
MaeIII GTNAC 2 cut(s) 1226, 1298
MalI GATC 5 cut(s) 252, 480, 855, 903, 926
MboI GATC 5 cut(s) 250, 478, 853, 901, 924
MboII GAAGA 7 cut(s) 234, 368, 628, 926, 949, 1188, 1304
MfeI CAATTG 1 cut(s) 814
MflI RGATCY 2 cut(s) 250, 901
MhlI GDGCHC 3 cut(s) 529, 934, 1502
MluCI AATT 8 cut(s) 276, 484, 661, 814, 864, 1112, 1366, 1380
MlyI GAGTC 1 cut(s) 14
Mph1103I ATGCAT 1 cut(s) 439
MseI TTAA 6 cut(s) 153, 539, 630, 749, 1046, 1076
MslI CAYNNNNRTG 1 cut(s) 759
MspA1I CMGCKG 3 cut(s) 296, 1429, 1535
MspCI CTTAAG 1 cut(s) 629
MspI CCGG 2 cut(s) 1200, 1358
MspR9I CCNGG 6 cut(s) 230, 966, 975, 1125, 1335, 1478
MunI CAATTG 1 cut(s) 814
MvaI CCWGG 6 cut(s) 230, 966, 975, 1125, 1335, 1478
MvnI CGCG 4 cut(s) 196, 442, 1429, 1535
MwoI GCNNNNNNNGC 9 cut(s) 71, 176, 587, 653, 1382, 1488, 1497, 1532, 1538
NcoI CCATGG 1 cut(s) 1024
NdeII GATC 5 cut(s) 250, 478, 853, 901, 924
NlaIII CATG 5 cut(s) 355, 537, 661, 793, 1028
NlaIV GGNNCC 1 cut(s) 972
NmuCI GTSAC 2 cut(s) 1226, 1298
NruI TCGCGA 1 cut(s) 442
NsiI ATGCAT 1 cut(s) 439
NspI RCATGY 1 cut(s) 793
PaeI GCATGC 1 cut(s) 793
PalAI GGCGCGCC 1 cut(s) 194
PauI GCGCGC 1 cut(s) 194
PcsI WCGNNNNNNNCGW 1 cut(s) 1563
PfeI GAWTC 3 cut(s) 47, 509, 1193
PfoI TCCNGGA 1 cut(s) 1123
PkrI GCNGC 5 cut(s) 73, 460, 649, 1387, 1534
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 1502
Psp6I CCWGG 6 cut(s) 228, 964, 973, 1123, 1333, 1476
PspFI CCCAGC 1 cut(s) 292
PspGI CCWGG 6 cut(s) 228, 964, 973, 1123, 1333, 1476
PspN4I GGNNCC 1 cut(s) 972
PspPI GGNCC 2 cut(s) 834, 1480
PsuI RGATCY 2 cut(s) 250, 901
PteI GCGCGC 1 cut(s) 194
PvuII CAGCTG 1 cut(s) 296
RruI TCGCGA 1 cut(s) 442
RsaI GTAC 2 cut(s) 407, 1165
RsaNI GTAC 2 cut(s) 406, 1164
RseI CAYNNNNRTG 1 cut(s) 759
SacI GAGCTC 1 cut(s) 1502
SacII CCGCGG 2 cut(s) 1430, 1536
SaqAI TTAA 6 cut(s) 153, 539, 630, 749, 1046, 1076
SatI GCNGC 5 cut(s) 72, 459, 648, 1386, 1533
Sau3AI GATC 5 cut(s) 250, 478, 853, 901, 924
Sau96I GGNCC 2 cut(s) 834, 1480
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 6 cut(s) 230, 966, 975, 1125, 1335, 1478
SduI GDGCHC 3 cut(s) 529, 934, 1502
SexAI ACCWGGT 1 cut(s) 973
SfaNI GCATC 5 cut(s) 424, 446, 895, 1397, 1528
Sfr303I CCGCGG 2 cut(s) 1430, 1536
SgrBI CCGCGG 2 cut(s) 1430, 1536
SgsI GGCGCGCC 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 759
SmlI CTYRAG 3 cut(s) 374, 629, 1222
SmoI CTYRAG 3 cut(s) 374, 629, 1222
SpeI ACTAGT 1 cut(s) 1451
SphI GCATGC 1 cut(s) 793
Sse9I AATT 8 cut(s) 276, 484, 661, 814, 864, 1112, 1366, 1380
SsiI CCGC 8 cut(s) 113, 650, 1080, 1427, 1429, 1489, 1533, 1535
SspMI CTAG 4 cut(s) 711, 1016, 1371, 1452
SstI GAGCTC 1 cut(s) 1502
StyD4I CCNGG 6 cut(s) 228, 964, 973, 1123, 1333, 1476
StyI CCWWGG 1 cut(s) 1024
TaqI TCGA 5 cut(s) 8, 498, 520, 781, 1092
TasI AATT 8 cut(s) 276, 484, 661, 814, 864, 1112, 1366, 1380
TatI WGTACW 1 cut(s) 405
TauI GCSGC 1 cut(s) 1535
TfiI GAWTC 3 cut(s) 47, 509, 1193
Tru1I TTAA 6 cut(s) 153, 539, 630, 749, 1046, 1076
Tru9I TTAA 6 cut(s) 153, 539, 630, 749, 1046, 1076
TscAI CASTG 2 cut(s) 177, 934
TseFI GTSAC 2 cut(s) 1226, 1298
TseI GCWGC 4 cut(s) 71, 458, 647, 1385
Tsp45I GTSAC 2 cut(s) 1226, 1298
TspDTI ATGAA 3 cut(s) 123, 522, 674
TspGWI ACGGA 1 cut(s) 949
TspRI CASTG 2 cut(s) 177, 934
Vha464I CTTAAG 1 cut(s) 629
VneI GTGCAC 1 cut(s) 930
XagI CCTNNNNNAGG 1 cut(s) 1122
XapI RAATTY 2 cut(s) 864, 1112
XbaI TCTAGA 1 cut(s) 1370
XceI RCATGY 1 cut(s) 793
XcmI CCANNNNNNNNNTGG 2 cut(s) 578, 918
XspI CTAG 4 cut(s) 711, 1016, 1371, 1452
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.