MD02G1179600.v1.1

Maternal effect embryo arrest 59

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012315
Physical Location & Seq
Forward (+)
15959363 .. 15960738
1376 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1179600.v1.1.491

Sequence Viewer

Length: 594 bp
ATGTCTTCTGTTCGAATCCCCGTTTCACAATATGGATCCACCGCCAACTCAAACCAAAACGGAAGAAAGATCAAACCTTTGATGGTGGTGGGGCAGATGAAGCCGGTAAAGCCGAGCCGGAGCGACGAGGTGTTGTCACCCGATGAACAGCTCAAGATCGCCGAGCAGGTAAGGGCCCAGTTCGACTCTGCCGCCCCAAAGCGACCCATCAAGCCGAGCCGGAGCGACCCCGATTCAAACCCGGTTCCCTACTTCGTCGTCGACCAACAACCCACCATTCCTGAGCTGGACAAGTTTCGTTCTCTCGAGTCCCAATCCGCTGCTGTTATACTCTCAGCGCAGGGAGATCCCACGGTGGAAGATGAGTTTGTGGATACAGAGTACTACAAGGAACTCAATTCCATCGACAAACAGCATCACACGACCGGAACTGGGTTCATAAGAGCGGTGAGAGAAGAGGGCAGTGAAGTTGATGGGCTCCATCTTCATCTACCTGAAAGTCATGCGAGAGTGGGATCGAAGATCAAAAGCAACCCTGCAACAAATGATTGGACTCCAAGGAACGATGACGATGACGATGACGCGTGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

21.91

Weight (kDa)

5.03

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37300
fragaria_vesca FvH4_1g16910 FvH4_1g16910
malus_domestica MD02G1179600.v1.1
prunus_persica Prupe.6G227700_v2.0.a1 Prupe.6G227700_v2.0.a1
pyrus_communis pycom02g14560
rosa_chinensis RchiOBHm_Chr2g0106961
rosa_laevigata RLG00000017546
rosa_multiflora Rmu_co8001210.1_g000001 Rmu_sc0004344.1_g000020
rosa_roxburghii Rroxscaffold_2G00136710
rosa_rugosa Rorug02G0139500 Rorug02G0139600
rosa_samantha Rh2AG190600 Rh2BG202100 Rh2CG195100 Rh2DG197300
rosa_wichuraiana Rw2G015020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 157
AccBSI CCGCTC 1 cut(s) 446
AccI GTMKAC 1 cut(s) 261
AccII CGCG 1 cut(s) 584
AciI CCGC 4 cut(s) 42, 192, 318, 446
AclWI GGATC 4 cut(s) 30, 43, 341, 523
AfaI GTAC 1 cut(s) 383
AfiI CCNNNNNNNGG 1 cut(s) 432
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 1 cut(s) 237
AluBI AGCT 2 cut(s) 151, 286
AluI AGCT 2 cut(s) 151, 286
AlwI GGATC 4 cut(s) 30, 43, 341, 523
Ama87I CYCGRG 1 cut(s) 305
AoxI GGCC 1 cut(s) 174
ApaI GGGCCC 1 cut(s) 178
ApeKI GCWGC 1 cut(s) 320
AspLEI GCGC 1 cut(s) 340
AspS9I GGNCC 2 cut(s) 174, 175
AsuC2I CCSGG 1 cut(s) 242
AsuHPI GGTGA 2 cut(s) 129, 460
AsuII TTCGAA 1 cut(s) 13
AvaI CYCGRG 1 cut(s) 305
BaeGI GKGCMC 1 cut(s) 178
BamHI GGATCC 1 cut(s) 35
BanII GRGCYC 2 cut(s) 178, 480
BbvI GCAGC 1 cut(s) 307
BccI CCATC 5 cut(s) 76, 215, 410, 467, 489
BciVI GTATCC 1 cut(s) 367
BcnI CCSGG 1 cut(s) 242
BfuAI ACCTGC 1 cut(s) 157
BfuI GTATCC 1 cut(s) 367
BisI GCNGC 2 cut(s) 192, 321
BlsI GCNGC 2 cut(s) 193, 322
BmcAI AGTACT 1 cut(s) 383
Bme1390I CCNGG 1 cut(s) 242
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 174, 175
BmiI GGNNCC 4 cut(s) 37, 176, 246, 479
BmrFI CCNGG 1 cut(s) 242
BmrI ACTGGG 2 cut(s) 172, 441
BmsI GCATC 1 cut(s) 424
BmuI ACTGGG 2 cut(s) 172, 441
Bpu10I CCTNAGC 1 cut(s) 282
Bpu14I TTCGAA 1 cut(s) 13
BpuEI CTTGAG 1 cut(s) 137
BpuMI CCSGG 1 cut(s) 242
BsaJI CCNNGG 2 cut(s) 351, 557
BsaWI WCCGGW 1 cut(s) 425
Bsc4I CCNNNNNNNGG 1 cut(s) 432
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 2 cut(s) 178, 436
BseDI CCNNGG 2 cut(s) 351, 557
BseLI CCNNNNNNNGG 1 cut(s) 432
BseMII CTCAG 2 cut(s) 273, 348
BseNI ACTGG 2 cut(s) 178, 436
BseSI GKGCMC 1 cut(s) 178
BseXI GCAGC 1 cut(s) 307
Bsh1236I CGCG 1 cut(s) 584
Bsh1285I CGRYCG 1 cut(s) 426
BshFI GGCC 1 cut(s) 176
BsiEI CGRYCG 1 cut(s) 426
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 5 cut(s) 104, 118, 220, 242, 426
BslFI GGGAC 1 cut(s) 295
BslI CCNNNNNNNGG 1 cut(s) 432
BsmFI GGGAC 1 cut(s) 295
BsnI GGCC 1 cut(s) 176
BsoBI CYCGRG 1 cut(s) 305
Bsp119I TTCGAA 1 cut(s) 13
Bsp120I GGGCCC 1 cut(s) 174
Bsp1286I GDGCHC 2 cut(s) 178, 480
Bsp143I GATC 6 cut(s) 35, 69, 156, 346, 515, 522
BspACI CCGC 4 cut(s) 42, 192, 318, 446
BspANI GGCC 1 cut(s) 176
BspCNI CTCAG 2 cut(s) 274, 347
BspFNI CGCG 1 cut(s) 584
BspLI GGNNCC 4 cut(s) 37, 176, 246, 479
BspMI ACCTGC 1 cut(s) 157
BspPI GGATC 4 cut(s) 30, 43, 341, 523
BspT104I TTCGAA 1 cut(s) 13
BsrBI CCGCTC 1 cut(s) 446
BsrFI RCCGGY 1 cut(s) 103
BsrI ACTGG 2 cut(s) 178, 436
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 2 cut(s) 351, 557
BssMI GATC 6 cut(s) 35, 69, 156, 346, 515, 522
BssT1I CCWWGG 1 cut(s) 557
Bst4CI ACNGT 1 cut(s) 355
Bst6I CTCTTC 1 cut(s) 450
BstBI TTCGAA 1 cut(s) 13
BstDEI CTNAG 2 cut(s) 282, 334
BstDSI CCRYGG 1 cut(s) 351
BstFNI CGCG 1 cut(s) 584
BstHHI GCGC 1 cut(s) 340
BstKTI GATC 6 cut(s) 38, 72, 159, 349, 518, 525
BstMBI GATC 6 cut(s) 35, 69, 156, 346, 515, 522
BstMCI CGRYCG 1 cut(s) 426
BstMWI GCNNNNNNNGC 2 cut(s) 100, 109
BstSCI CCNGG 1 cut(s) 240
BstSLI GKGCMC 1 cut(s) 178
BstUI CGCG 1 cut(s) 584
BstV1I GCAGC 1 cut(s) 307
BstX2I RGATCY 2 cut(s) 35, 346
BstYI RGATCY 2 cut(s) 35, 346
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 1 cut(s) 176
BtgI CCRYGG 1 cut(s) 351
BtsI GCAGTG 1 cut(s) 469
BtsIMutI CAGTG 1 cut(s) 469
BveI ACCTGC 1 cut(s) 157
CfoI GCGC 1 cut(s) 340
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 2 cut(s) 174, 175
Csp6I GTAC 1 cut(s) 382
CviAII CATG 1 cut(s) 503
CviJI RGCY 9 cut(s) 103, 112, 117, 151, 176, 214, 219, 286, 478
CviKI_1 RGCY 9 cut(s) 103, 112, 117, 151, 176, 214, 219, 286, 478
CviQI GTAC 1 cut(s) 382
DdeI CTNAG 2 cut(s) 282, 334
DpnI GATC 6 cut(s) 37, 71, 158, 348, 517, 524
DpnII GATC 6 cut(s) 35, 69, 156, 346, 515, 522
Eam1104I CTCTTC 1 cut(s) 450
EarI CTCTTC 1 cut(s) 450
Eco130I CCWWGG 1 cut(s) 557
Eco24I GRGCYC 2 cut(s) 178, 480
Eco88I CYCGRG 1 cut(s) 305
EcoO109I RGGNCCY 1 cut(s) 174
EcoT14I CCWWGG 1 cut(s) 557
EcoT38I GRGCYC 2 cut(s) 178, 480
ErhI CCWWGG 1 cut(s) 557
FaeI CATG 1 cut(s) 506
FaiI YATR 4 cut(s) 33, 329, 440, 504
FaqI GGGAC 1 cut(s) 295
FatI CATG 1 cut(s) 502
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 2 cut(s) 192, 321
FriOI GRGCYC 2 cut(s) 178, 480
Fsp4HI GCNGC 2 cut(s) 192, 321
GlaI GCGC 1 cut(s) 339
GluI GCNGC 2 cut(s) 192, 321
HaeIII GGCC 1 cut(s) 176
HapII CCGG 5 cut(s) 104, 118, 220, 242, 426
HhaI GCGC 1 cut(s) 340
Hin1II CATG 1 cut(s) 506
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HinfI GANTC 5 cut(s) 15, 185, 233, 308, 553
HpaII CCGG 5 cut(s) 104, 118, 220, 242, 426
HphI GGTGA 2 cut(s) 129, 460
Hpy166II GTNNAC 1 cut(s) 262
Hpy188III TCNNGA 3 cut(s) 154, 281, 305
Hpy8I GTNNAC 1 cut(s) 262
Hpy99I CGWCG 3 cut(s) 128, 260, 263
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4V TGCA 1 cut(s) 539
HpyF10VI GCNNNNNNNGC 2 cut(s) 100, 109
HpyF3I CTNAG 2 cut(s) 282, 334
Hsp92II CATG 1 cut(s) 506
HspAI GCGC 1 cut(s) 338
Kzo9I GATC 6 cut(s) 35, 69, 156, 346, 515, 522
LmnI GCTCC 3 cut(s) 120, 222, 483
Lsp1109I GCAGC 1 cut(s) 307
LweI GCATC 1 cut(s) 424
MaeIII GTNAC 1 cut(s) 135
MalI GATC 6 cut(s) 37, 71, 158, 348, 517, 524
MbiI CCGCTC 1 cut(s) 446
MboI GATC 6 cut(s) 35, 69, 156, 346, 515, 522
MboII GAAGA 5 cut(s) 75, 371, 467, 476, 532
MflI RGATCY 2 cut(s) 35, 346
MhlI GDGCHC 2 cut(s) 178, 480
MluCI AATT 1 cut(s) 397
MluI ACGCGT 1 cut(s) 582
MlyI GAGTC 3 cut(s) 179, 317, 547
MnlI CCTC 2 cut(s) 121, 451
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 5 cut(s) 104, 118, 220, 242, 426
MspR9I CCNGG 1 cut(s) 242
MvnI CGCG 1 cut(s) 584
MwoI GCNNNNNNNGC 2 cut(s) 100, 109
NciI CCSGG 1 cut(s) 242
NdeII GATC 6 cut(s) 35, 69, 156, 346, 515, 522
NlaIII CATG 1 cut(s) 506
NlaIV GGNNCC 4 cut(s) 37, 176, 246, 479
NmeAIII GCCGAG 3 cut(s) 138, 187, 240
NmuCI GTSAC 1 cut(s) 135
NspV TTCGAA 1 cut(s) 13
PaeR7I CTCGAG 1 cut(s) 305
PfeI GAWTC 2 cut(s) 15, 233
PkrI GCNGC 2 cut(s) 193, 322
PleI GAGTC 3 cut(s) 179, 316, 547
PpsI GAGTC 3 cut(s) 179, 316, 547
PspN4I GGNNCC 4 cut(s) 37, 176, 246, 479
PspOMI GGGCCC 1 cut(s) 174
PspPI GGNCC 2 cut(s) 174, 175
PsuI RGATCY 2 cut(s) 35, 346
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
SalI GTCGAC 1 cut(s) 260
SatI GCNGC 2 cut(s) 192, 321
Sau3AI GATC 6 cut(s) 35, 69, 156, 346, 515, 522
Sau96I GGNCC 2 cut(s) 174, 175
ScaI AGTACT 1 cut(s) 383
SchI GAGTC 3 cut(s) 179, 317, 547
ScrFI CCNGG 1 cut(s) 242
SduI GDGCHC 2 cut(s) 178, 480
SetI ASST 6 cut(s) 79, 132, 153, 171, 288, 496
SfaNI GCATC 1 cut(s) 424
Sfr274I CTCGAG 1 cut(s) 305
SfuI TTCGAA 1 cut(s) 13
SlaI CTCGAG 1 cut(s) 305
SmlI CTYRAG 2 cut(s) 152, 305
SmoI CTYRAG 2 cut(s) 152, 305
Sse9I AATT 1 cut(s) 397
SsiI CCGC 4 cut(s) 42, 192, 318, 446
StyD4I CCNGG 1 cut(s) 240
StyI CCWWGG 1 cut(s) 557
TaaI ACNGT 1 cut(s) 355
TaqI TCGA 6 cut(s) 13, 183, 261, 306, 405, 518
TasI AATT 1 cut(s) 397
TatI WGTACW 1 cut(s) 381
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 2 cut(s) 15, 233
TscAI CASTG 1 cut(s) 469
TseFI GTSAC 1 cut(s) 135
TseI GCWGC 1 cut(s) 320
Tsp45I GTSAC 1 cut(s) 135
TspDTI ATGAA 4 cut(s) 113, 159, 427, 476
TspGWI ACGGA 1 cut(s) 75
TspRI CASTG 1 cut(s) 469
XcmI CCANNNNNNNNNTGG 1 cut(s) 283
XhoI CTCGAG 1 cut(s) 305
XmiI GTMKAC 1 cut(s) 261
ZrmI AGTACT 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.