Rroxscaffold_2G00136710

Maternal effect embryo arrest 59

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012315
Physical Location & Seq
Reverse (-)
74383493 .. 74384665
1173 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00136710.1

Sequence Viewer

Length: 531 bp
ATGGTGGGTCAGTGGATGGTGGCGAAGCCAAGCCGGAGCGACGACGTTTTGGATGCCGACGAACAGCTCAGGATCGCCACCCAGATCAAAGCTCAGTTCGACTCTGCAGCGCCAAAGCGACCCATGAAGCCGAATCGAAGCGAACCCGATTCACCAGCTCCAGCTCTCTCCATCGTTGACCCAACCAACATTCCTGAGCTTCACAAGCTTCGTACTCTTCAGTCTCAATCCCATGTCAGAATCCCAGATGAGGGAGCTAGCATCATGGTACAAGATGAGTTTGTGGATACCCAATATTACAAGGAACTGAATTCCATTGACAAACAGCATCACATGACTGGGACTGGGTTTATAAGGGTGGAGAGGGAAGAAGAGGGAAATGAAGATGGCTATGATATTCAGTTGAATGGAATTCATGGTGGTTCAAATGGTATAGTGCGGGCTGGTTTCAGAAGCAACCCTGCAACAAATGACTGGATTCCTGAGACTCTGGTCTTCATTTCCTCAAAGCCAAATCGAAGTGAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

19.68

Weight (kDa)

5.09

Isoelectric Point (pI)

50.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37300
fragaria_vesca FvH4_1g16910 FvH4_1g16910
malus_domestica MD02G1179600.v1.1
prunus_persica Prupe.6G227700_v2.0.a1 Prupe.6G227700_v2.0.a1
pyrus_communis pycom02g14560
rosa_chinensis RchiOBHm_Chr2g0106961
rosa_laevigata RLG00000017546
rosa_multiflora Rmu_co8001210.1_g000001 Rmu_sc0004344.1_g000020
rosa_roxburghii Rroxscaffold_2G00136710
rosa_rugosa Rorug02G0139500 Rorug02G0139600
rosa_samantha Rh2AG190600 Rh2BG202100 Rh2CG195100 Rh2DG197300
rosa_wichuraiana Rw2G015020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 353
AciI CCGC 1 cut(s) 439
AclWI GGATC 1 cut(s) 80
AcsI RAATTY 2 cut(s) 310, 411
AcuI CTGAAG 1 cut(s) 203
AfaI GTAC 2 cut(s) 214, 270
AfiI CCNNNNNNNGG 2 cut(s) 250, 251
AgsI TTSAA 2 cut(s) 406, 426
AluBI AGCT 7 cut(s) 67, 92, 158, 164, 199, 208, 257
AluI AGCT 7 cut(s) 67, 92, 158, 164, 199, 208, 257
Alw26I GTCTC 2 cut(s) 228, 479
AlwI GGATC 1 cut(s) 80
ApeKI GCWGC 1 cut(s) 107
ApoI RAATTY 2 cut(s) 310, 411
AspLEI GCGC 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 144
AsuNHI GCTAGC 1 cut(s) 257
BbsI GAAGAC 1 cut(s) 487
BbvI GCAGC 1 cut(s) 119
BccI CCATC 3 cut(s) 10, 179, 380
BciVI GTATCC 1 cut(s) 280
BcoDI GTCTC 2 cut(s) 228, 479
BfaI CTAG 1 cut(s) 258
BfmI CTRYAG 1 cut(s) 105
BfoI RGCGCY 1 cut(s) 113
BfuI GTATCC 1 cut(s) 280
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
BmrI ACTGGG 2 cut(s) 348, 354
BmsI GCATC 3 cut(s) 43, 270, 337
BmtI GCTAGC 1 cut(s) 261
BmuI ACTGGG 2 cut(s) 348, 354
BoxI GACNNNNGTC 1 cut(s) 491
BpiI GAAGAC 1 cut(s) 487
BpmI CTGGAG 1 cut(s) 144
Bpu10I CCTNAGC 2 cut(s) 68, 195
Bsc4I CCNNNNNNNGG 2 cut(s) 250, 251
Bse1I ACTGG 3 cut(s) 343, 349, 479
BseGI GGATG 2 cut(s) 21, 58
BseLI CCNNNNNNNGG 2 cut(s) 250, 251
BseMII CTCAG 4 cut(s) 82, 107, 186, 474
BseNI ACTGG 3 cut(s) 343, 349, 479
BseXI GCAGC 1 cut(s) 119
BsiSI CCGG 1 cut(s) 34
BslFI GGGAC 1 cut(s) 355
BslI CCNNNNNNNGG 2 cut(s) 250, 251
BsmAI GTCTC 2 cut(s) 228, 479
BsmFI GGGAC 1 cut(s) 355
Bsp143I GATC 2 cut(s) 72, 84
BspACI CCGC 1 cut(s) 439
BspCNI CTCAG 4 cut(s) 81, 106, 187, 475
BspMAI CTGCAG 1 cut(s) 109
BspOI GCTAGC 1 cut(s) 261
BspPI GGATC 1 cut(s) 80
BsrI ACTGG 3 cut(s) 343, 349, 479
BssMI GATC 2 cut(s) 72, 84
Bst6I CTCTTC 2 cut(s) 222, 366
BstC8I GCNNGC 2 cut(s) 259, 441
BstDEI CTNAG 4 cut(s) 68, 93, 195, 483
BstF5I GGATG 2 cut(s) 21, 58
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 2 cut(s) 75, 87
BstMAI GTCTC 2 cut(s) 228, 479
BstMBI GATC 2 cut(s) 72, 84
BstMWI GCNNNNNNNGC 1 cut(s) 205
BstPAI GACNNNNGTC 1 cut(s) 491
BstSFI CTRYAG 1 cut(s) 105
BstV1I GCAGC 1 cut(s) 119
BstV2I GAAGAC 1 cut(s) 487
BsuI GTATCC 1 cut(s) 280
BtsCI GGATG 2 cut(s) 21, 58
BtsIMutI CAGTG 1 cut(s) 17
Cac8I GCNNGC 2 cut(s) 259, 441
CfoI GCGC 1 cut(s) 112
Csp6I GTAC 2 cut(s) 213, 269
CviAII CATG 5 cut(s) 124, 233, 265, 334, 416
CviQI GTAC 2 cut(s) 213, 269
DdeI CTNAG 4 cut(s) 68, 93, 195, 483
DpnI GATC 2 cut(s) 74, 86
DpnII GATC 2 cut(s) 72, 84
Eam1104I CTCTTC 2 cut(s) 222, 366
EarI CTCTTC 2 cut(s) 222, 366
Eco57I CTGAAG 1 cut(s) 203
EcoRI GAATTC 2 cut(s) 310, 411
FaeI CATG 5 cut(s) 127, 236, 268, 337, 419
FaiI YATR 8 cut(s) 125, 234, 266, 335, 353, 393, 417, 434
FaqI GGGAC 1 cut(s) 355
FatI CATG 5 cut(s) 123, 232, 264, 333, 415
FauI CCCGC 1 cut(s) 432
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 2 cut(s) 28, 65
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 1 cut(s) 258
GlaI GCGC 1 cut(s) 111
GluI GCNGC 1 cut(s) 108
GsuI CTGGAG 1 cut(s) 144
HaeII RGCGCY 1 cut(s) 113
HapII CCGG 1 cut(s) 34
HhaI GCGC 1 cut(s) 112
Hin1II CATG 5 cut(s) 127, 236, 268, 337, 419
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 6 cut(s) 101, 133, 149, 240, 478, 487
HpaII CCGG 1 cut(s) 34
HphI GGTGA 1 cut(s) 144
Hpy166II GTNNAC 1 cut(s) 178
Hpy188I TCNGA 2 cut(s) 239, 452
Hpy188III TCNNGA 3 cut(s) 70, 194, 482
Hpy8I GTNNAC 1 cut(s) 178
Hpy99I CGWCG 3 cut(s) 44, 47, 62
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 2 cut(s) 107, 464
HpyF10VI GCNNNNNNNGC 1 cut(s) 205
HpyF3I CTNAG 4 cut(s) 68, 93, 195, 483
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 5 cut(s) 127, 236, 268, 337, 419
HspAI GCGC 1 cut(s) 110
Kzo9I GATC 2 cut(s) 72, 84
LmnI GCTCC 3 cut(s) 36, 163, 254
Lsp1109I GCAGC 1 cut(s) 119
LweI GCATC 3 cut(s) 43, 270, 337
MaeI CTAG 1 cut(s) 258
MaeII ACGT 1 cut(s) 45
MalI GATC 2 cut(s) 74, 86
MboI GATC 2 cut(s) 72, 84
MboII GAAGA 5 cut(s) 209, 380, 383, 395, 487
MluCI AATT 2 cut(s) 310, 411
MlyI GAGTC 2 cut(s) 95, 481
MnlI CCTC 4 cut(s) 244, 357, 367, 514
MspI CCGG 1 cut(s) 34
MwoI GCNNNNNNNGC 1 cut(s) 205
NdeII GATC 2 cut(s) 72, 84
NheI GCTAGC 1 cut(s) 257
NlaIII CATG 5 cut(s) 127, 236, 268, 337, 419
PfeI GAWTC 4 cut(s) 133, 149, 240, 478
PkrI GCNGC 1 cut(s) 109
PleI GAGTC 2 cut(s) 95, 481
PpsI GAGTC 2 cut(s) 95, 481
PshAI GACNNNNGTC 1 cut(s) 491
PsiI TTATAA 1 cut(s) 353
PstI CTGCAG 1 cut(s) 109
RsaI GTAC 2 cut(s) 214, 270
RsaNI GTAC 2 cut(s) 213, 269
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 2 cut(s) 72, 84
SchI GAGTC 2 cut(s) 95, 481
SetI ASST 8 cut(s) 48, 69, 94, 160, 166, 201, 210, 259
SfaNI GCATC 3 cut(s) 43, 270, 337
SfcI CTRYAG 1 cut(s) 105
Sse9I AATT 2 cut(s) 310, 411
SsiI CCGC 1 cut(s) 439
SspI AATATT 1 cut(s) 296
SspMI CTAG 1 cut(s) 258
TaiI ACGT 1 cut(s) 48
TaqI TCGA 3 cut(s) 99, 136, 517
TasI AATT 2 cut(s) 310, 411
TfiI GAWTC 4 cut(s) 133, 149, 240, 478
TscAI CASTG 1 cut(s) 17
TseI GCWGC 1 cut(s) 107
TspDTI ATGAA 4 cut(s) 140, 396, 404, 487
TspRI CASTG 1 cut(s) 17
XapI RAATTY 2 cut(s) 310, 411
XspI CTAG 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.