Rorug02G0139600

Maternal effect embryo arrest 59

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0012315
Physical Location & Seq
Reverse (-)
12287817 .. 12289385
1569 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0139600.1

Sequence Viewer

Length: 645 bp
ATGGCCTCACAGTGGCTAGAACTTGGGCTTTCAGTGACGGAGTCTTTTGGGTTTGGTTTGAAGGGGTTGGATTTTGTTATAGCTGAGGCTAGAAGAAACCCTGTTGTTAAGGGTTTCTACAAGAACCATGTCAGTACTGTTCTTAACAGATACAACATCTACACTAGAGTTCATTACAAAGATGATCCAACAGTTATGGCTTGGGAGCTTATGAATGAGCCTAGATGCATATCAGATCCTTCAGGAAGGACAATTCAGGCTTGGATAAGGGAAATGGCTTCCCATGTGAAGTCAATAGATAGAAATCATTTACTGGAAGCTGGTTTAGAGGGATTTTATGGACAATCAACACCTCAGAGGATGAGACTCAATCCTGGTTTCCAAATAGGAACAGACTTCATTGCAAATAATAAGATCCCTGGGATTGATTTTGCAACAGTTCACTCCTATCCAGATCAATGGTTGTCTAGCTCAAACGATCAATATCAACTGTTTTTCTTGAACAACTGGCTTAATACTCACATCCAAGATTCACAGTACATTCTTCGAAAGCCGATCTTCATCGCAGAATTTGGGAAATCTAGGAAAGATCCTGGTTTCAACACGTACCAAAGAGACCTGCTCTTCAACACCGTGTACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

214

Amino Acids

24.84

Weight (kDa)

7.85

Isoelectric Point (pI)

33.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH5_mannosidase PF26410 36 - 212 2.6e-27 GH family 5 beta-mannosidase-like
Cellulase PF00150 58 - 198 3.4e-08 Cellulase (glycosyl hydrolase family 5)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37300
fragaria_vesca FvH4_1g16910 FvH4_1g16910
malus_domestica MD02G1179600.v1.1
prunus_persica Prupe.6G227700_v2.0.a1 Prupe.6G227700_v2.0.a1
pyrus_communis pycom02g14560
rosa_chinensis RchiOBHm_Chr2g0106961
rosa_laevigata RLG00000017546
rosa_multiflora Rmu_co8001210.1_g000001 Rmu_sc0004344.1_g000020
rosa_roxburghii Rroxscaffold_2G00136710
rosa_rugosa Rorug02G0139500 Rorug02G0139600
rosa_samantha Rh2AG190600 Rh2BG202100 Rh2CG195100 Rh2DG197300
rosa_wichuraiana Rw2G015020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 627
AclWI GGATC 4 cut(s) 179, 230, 409, 584
AcsI RAATTY 1 cut(s) 569
AcuI CTGAAG 1 cut(s) 225
AfaI GTAC 4 cut(s) 136, 539, 608, 638
AfiI CCNNNNNNNGG 1 cut(s) 12
AflIII ACRYGT 1 cut(s) 603
AgsI TTSAA 4 cut(s) 61, 502, 601, 628
AjnI CCWGG 3 cut(s) 373, 418, 592
AluBI AGCT 4 cut(s) 83, 208, 320, 471
AluI AGCT 4 cut(s) 83, 208, 320, 471
Alw26I GTCTC 2 cut(s) 358, 609
AlwI GGATC 4 cut(s) 179, 230, 409, 584
AoxI GGCC 1 cut(s) 3
ApoI RAATTY 1 cut(s) 569
AsuII TTCGAA 1 cut(s) 547
BbvCI CCTCAGC 1 cut(s) 84
BciT130I CCWGG 3 cut(s) 375, 420, 594
BcoDI GTCTC 2 cut(s) 358, 609
BfaI CTAG 7 cut(s) 17, 90, 165, 222, 468, 582, 643
BfuAI ACCTGC 1 cut(s) 627
BmcAI AGTACT 1 cut(s) 136
Bme1390I CCNGG 3 cut(s) 375, 420, 594
BmrFI CCNGG 3 cut(s) 375, 420, 594
BmsI GCATC 1 cut(s) 215
BplI GAGNNNNNCTC 2 cut(s) 606, 638
Bpu10I CCTNAGC 1 cut(s) 84
Bpu14I TTCGAA 1 cut(s) 547
BsaAI YACGTR 1 cut(s) 606
BsaBI GATNNNNATC 4 cut(s) 229, 303, 483, 560
BsaI GGTCTC 1 cut(s) 609
BsaJI CCNNGG 2 cut(s) 418, 419
Bsc4I CCNNNNNNNGG 1 cut(s) 12
Bse1I ACTGG 2 cut(s) 318, 512
Bse3DI GCAATG 1 cut(s) 399
Bse8I GATNNNNATC 4 cut(s) 229, 303, 483, 560
BseBI CCWGG 3 cut(s) 375, 420, 594
BseDI CCNNGG 2 cut(s) 418, 419
BseGI GGATG 2 cut(s) 366, 522
BseJI GATNNNNATC 4 cut(s) 229, 303, 483, 560
BseLI CCNNNNNNNGG 1 cut(s) 12
BseMI GCAATG 1 cut(s) 399
BseMII CTCAG 2 cut(s) 75, 368
BseNI ACTGG 2 cut(s) 318, 512
BshFI GGCC 1 cut(s) 5
BslI CCNNNNNNNGG 1 cut(s) 12
BsmAI GTCTC 2 cut(s) 358, 609
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 609
Bsp119I TTCGAA 1 cut(s) 547
Bsp143I GATC 7 cut(s) 184, 235, 414, 454, 478, 555, 589
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 76, 367
BspMI ACCTGC 1 cut(s) 627
BspPI GGATC 4 cut(s) 179, 230, 409, 584
BspQI GCTCTTC 1 cut(s) 629
BspT104I TTCGAA 1 cut(s) 547
BspTNI GGTCTC 1 cut(s) 609
BsrDI GCAATG 1 cut(s) 399
BsrI ACTGG 2 cut(s) 318, 512
BssECI CCNNGG 2 cut(s) 418, 419
BssMI GATC 7 cut(s) 184, 235, 414, 454, 478, 555, 589
Bst2UI CCWGG 3 cut(s) 375, 420, 594
Bst4CI ACNGT 7 cut(s) 12, 139, 193, 439, 492, 537, 634
Bst6I CTCTTC 1 cut(s) 629
BstBAI YACGTR 1 cut(s) 606
BstBI TTCGAA 1 cut(s) 547
BstDEI CTNAG 2 cut(s) 84, 354
BstF5I GGATG 2 cut(s) 366, 522
BstKTI GATC 7 cut(s) 187, 238, 417, 457, 481, 558, 592
BstMAI GTCTC 2 cut(s) 358, 609
BstMBI GATC 7 cut(s) 184, 235, 414, 454, 478, 555, 589
BstNI CCWGG 3 cut(s) 375, 420, 594
BstSCI CCNGG 3 cut(s) 373, 418, 592
BstX2I RGATCY 3 cut(s) 235, 414, 589
BstXI CCANNNNNNTGG 1 cut(s) 459
BstYI RGATCY 3 cut(s) 235, 414, 589
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 547
BtsCI GGATG 2 cut(s) 366, 522
BtsIMutI CAGTG 2 cut(s) 17, 39
BveI ACCTGC 1 cut(s) 627
Csp6I GTAC 4 cut(s) 135, 538, 607, 637
CviAII CATG 2 cut(s) 128, 284
CviQI GTAC 4 cut(s) 135, 538, 607, 637
DdeI CTNAG 2 cut(s) 84, 354
DpnI GATC 7 cut(s) 186, 237, 416, 456, 480, 557, 591
DpnII GATC 7 cut(s) 184, 235, 414, 454, 478, 555, 589
Eam1104I CTCTTC 1 cut(s) 629
EarI CTCTTC 1 cut(s) 629
Eco31I GGTCTC 1 cut(s) 609
Eco57I CTGAAG 1 cut(s) 225
EcoRII CCWGG 3 cut(s) 373, 418, 592
EcoT22I ATGCAT 1 cut(s) 230
FaeI CATG 2 cut(s) 131, 287
FaiI YATR 7 cut(s) 80, 129, 197, 212, 230, 285, 339
FalI AAGNNNNNCTT 2 cut(s) 542, 574
FatI CATG 2 cut(s) 127, 283
FokI GGATG 2 cut(s) 373, 509
FspBI CTAG 7 cut(s) 17, 90, 165, 222, 468, 582, 643
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 2 cut(s) 131, 287
HinfI GANTC 3 cut(s) 41, 366, 530
Hpy166II GTNNAC 2 cut(s) 442, 637
Hpy188I TCNGA 2 cut(s) 235, 357
Hpy188III TCNNGA 3 cut(s) 243, 452, 499
Hpy8I GTNNAC 2 cut(s) 442, 637
HpyAV CCTTC 3 cut(s) 55, 240, 249
HpyCH4III ACNGT 7 cut(s) 12, 139, 193, 439, 492, 537, 634
HpyCH4IV ACGT 1 cut(s) 605
HpyCH4V TGCA 3 cut(s) 228, 404, 434
HpyF3I CTNAG 2 cut(s) 84, 354
HpySE526I ACGT 1 cut(s) 605
Hsp92II CATG 2 cut(s) 131, 287
Kzo9I GATC 7 cut(s) 184, 235, 414, 454, 478, 555, 589
LguI GCTCTTC 1 cut(s) 629
LmnI GCTCC 1 cut(s) 205
LweI GCATC 1 cut(s) 215
MaeI CTAG 7 cut(s) 17, 90, 165, 222, 468, 582, 643
MaeII ACGT 1 cut(s) 605
MaeIII GTNAC 1 cut(s) 34
MalI GATC 7 cut(s) 186, 237, 416, 456, 480, 557, 591
MboI GATC 7 cut(s) 184, 235, 414, 454, 478, 555, 589
MboII GAAGA 4 cut(s) 105, 536, 550, 616
MflI RGATCY 3 cut(s) 235, 414, 589
MluCI AATT 2 cut(s) 252, 569
MlyI GAGTC 2 cut(s) 50, 360
MmeI TCCRAC 2 cut(s) 48, 212
MnlI CCTC 5 cut(s) 16, 79, 322, 351, 363
Mph1103I ATGCAT 1 cut(s) 230
MseI TTAA 3 cut(s) 108, 144, 513
MspR9I CCNGG 3 cut(s) 375, 420, 594
MvaI CCWGG 3 cut(s) 375, 420, 594
NdeII GATC 7 cut(s) 184, 235, 414, 454, 478, 555, 589
NlaIII CATG 2 cut(s) 131, 287
NmuCI GTSAC 1 cut(s) 34
NsiI ATGCAT 1 cut(s) 230
NspV TTCGAA 1 cut(s) 547
PasI CCCWGGG 1 cut(s) 419
PciSI GCTCTTC 1 cut(s) 629
PfeI GAWTC 1 cut(s) 530
PflFI GACNNNGTC 1 cut(s) 40
PleI GAGTC 2 cut(s) 49, 360
PpsI GAGTC 2 cut(s) 49, 360
Ppu21I YACGTR 1 cut(s) 606
Psp6I CCWGG 3 cut(s) 373, 418, 592
PspGI CCWGG 3 cut(s) 373, 418, 592
PsuI RGATCY 3 cut(s) 235, 414, 589
PsyI GACNNNGTC 1 cut(s) 40
RsaI GTAC 4 cut(s) 136, 539, 608, 638
RsaNI GTAC 4 cut(s) 135, 538, 607, 637
SapI GCTCTTC 1 cut(s) 629
SaqAI TTAA 3 cut(s) 108, 144, 513
Sau3AI GATC 7 cut(s) 184, 235, 414, 454, 478, 555, 589
ScaI AGTACT 1 cut(s) 136
SchI GAGTC 2 cut(s) 50, 360
ScrFI CCNGG 3 cut(s) 375, 420, 594
SetI ASST 7 cut(s) 85, 210, 322, 355, 473, 608, 621
SfaNI GCATC 1 cut(s) 215
SfuI TTCGAA 1 cut(s) 547
Sse9I AATT 2 cut(s) 252, 569
SspMI CTAG 7 cut(s) 17, 90, 165, 222, 468, 582, 643
StyD4I CCNGG 3 cut(s) 373, 418, 592
TaaI ACNGT 7 cut(s) 12, 139, 193, 439, 492, 537, 634
TaiI ACGT 1 cut(s) 608
TaqI TCGA 1 cut(s) 547
TasI AATT 2 cut(s) 252, 569
TatI WGTACW 3 cut(s) 134, 537, 636
TfiI GAWTC 1 cut(s) 530
Tru1I TTAA 3 cut(s) 108, 144, 513
Tru9I TTAA 3 cut(s) 108, 144, 513
TscAI CASTG 2 cut(s) 17, 39
TseFI GTSAC 1 cut(s) 34
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 4 cut(s) 161, 227, 388, 550
TspGWI ACGGA 1 cut(s) 53
TspRI CASTG 2 cut(s) 17, 39
Tth111I GACNNNGTC 1 cut(s) 40
XapI RAATTY 1 cut(s) 569
XspI CTAG 7 cut(s) 17, 90, 165, 222, 468, 582, 643
ZrmI AGTACT 1 cut(s) 136
Zsp2I ATGCAT 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.