Rw2G015020

Maternal effect embryo arrest 59

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012315
Physical Location & Seq
Forward (+)
17317186 .. 17318333
1148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G015020.1

Sequence Viewer

Length: 513 bp
ATGGTGGGTCAGTGGATGGTGGCGAAGCCAAGCCGGAGCGACGACGTTTTGGATGCCGACGAACAGCTCAGGATCGCCACCCAGATCAGAGCTCAGTTCGACTCTGCAGCCCCAAAGCGACCCATGAAGCCGAATCGAAGCGAACCCGATTCACCAGCTCCAGCTCTCTCCATCGTTGACCCAACCAACATTCCTGAGCTTCACAAGCTTCGTACTCTTCAGTCTCAATCGCATGTCAGAATCTCAGATGAGGGAGCTAGCATCATGGTACAAGATGAGTTTGTGGATACCCAATATTACAAGGAACTGAATTCCATTGACAAACAGCATCACATGACTGGGACTGGGTTTATAAGGGTGGAGAGGGAAGAAGAGGGAAATGAAGATGGCTATGATATTCAGTTGAACGGAATTCATGGTGGTTCAAATGGTACAGTGCGGGCTGGTTTCAGAAGCAACCCTGCAACAAATGACTGGATTCCTAAGACTGATGAAGATCTGGTATGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

170

Amino Acids

19.08

Weight (kDa)

4.88

Isoelectric Point (pI)

49.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37300
fragaria_vesca FvH4_1g16910 FvH4_1g16910
malus_domestica MD02G1179600.v1.1
prunus_persica Prupe.6G227700_v2.0.a1 Prupe.6G227700_v2.0.a1
pyrus_communis pycom02g14560
rosa_chinensis RchiOBHm_Chr2g0106961
rosa_laevigata RLG00000017546
rosa_multiflora Rmu_co8001210.1_g000001 Rmu_sc0004344.1_g000020
rosa_roxburghii Rroxscaffold_2G00136710
rosa_rugosa Rorug02G0139500 Rorug02G0139600
rosa_samantha Rh2AG190600 Rh2BG202100 Rh2CG195100 Rh2DG197300
rosa_wichuraiana Rw2G015020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 353
AciI CCGC 1 cut(s) 439
AclWI GGATC 1 cut(s) 80
AcsI RAATTY 2 cut(s) 310, 411
AcuI CTGAAG 1 cut(s) 203
AfaI GTAC 3 cut(s) 214, 270, 433
AgsI TTSAA 2 cut(s) 406, 426
AluBI AGCT 7 cut(s) 67, 92, 158, 164, 199, 208, 257
AluI AGCT 7 cut(s) 67, 92, 158, 164, 199, 208, 257
Alw21I GWGCWC 1 cut(s) 94
Alw26I GTCTC 1 cut(s) 228
AlwI GGATC 1 cut(s) 80
ApeKI GCWGC 1 cut(s) 107
ApoI RAATTY 2 cut(s) 310, 411
AsuHPI GGTGA 1 cut(s) 144
AsuNHI GCTAGC 1 cut(s) 257
BanII GRGCYC 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 94
BbvI GCAGC 1 cut(s) 119
BccI CCATC 3 cut(s) 10, 179, 380
BciVI GTATCC 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 228
BfaI CTAG 1 cut(s) 258
BfmI CTRYAG 1 cut(s) 105
BfuI GTATCC 1 cut(s) 280
BglII AGATCT 1 cut(s) 496
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
BmrI ACTGGG 2 cut(s) 348, 354
BmsI GCATC 3 cut(s) 43, 270, 337
BmtI GCTAGC 1 cut(s) 261
BmuI ACTGGG 2 cut(s) 348, 354
BpmI CTGGAG 1 cut(s) 144
Bpu10I CCTNAGC 2 cut(s) 68, 195
BsaBI GATNNNNATC 1 cut(s) 495
Bse1I ACTGG 3 cut(s) 343, 349, 479
Bse8I GATNNNNATC 1 cut(s) 495
BseGI GGATG 2 cut(s) 21, 58
BseJI GATNNNNATC 1 cut(s) 495
BseMII CTCAG 4 cut(s) 82, 107, 186, 258
BseNI ACTGG 3 cut(s) 343, 349, 479
BseXI GCAGC 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 94
BsiSI CCGG 1 cut(s) 34
BslFI GGGAC 1 cut(s) 355
BsmAI GTCTC 1 cut(s) 228
BsmFI GGGAC 1 cut(s) 355
Bsp1286I GDGCHC 1 cut(s) 94
Bsp143I GATC 3 cut(s) 72, 84, 496
BspACI CCGC 1 cut(s) 439
BspCNI CTCAG 4 cut(s) 81, 106, 187, 257
BspMAI CTGCAG 1 cut(s) 109
BspOI GCTAGC 1 cut(s) 261
BspPI GGATC 1 cut(s) 80
BsrI ACTGG 3 cut(s) 343, 349, 479
BssMI GATC 3 cut(s) 72, 84, 496
Bst4CI ACNGT 1 cut(s) 436
Bst6I CTCTTC 2 cut(s) 222, 366
BstC8I GCNNGC 2 cut(s) 259, 441
BstDEI CTNAG 5 cut(s) 68, 93, 195, 244, 483
BstF5I GGATG 2 cut(s) 21, 58
BstKTI GATC 3 cut(s) 75, 87, 499
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 3 cut(s) 72, 84, 496
BstMWI GCNNNNNNNGC 1 cut(s) 205
BstNSI RCATGY 1 cut(s) 236
BstSFI CTRYAG 1 cut(s) 105
BstV1I GCAGC 1 cut(s) 119
BstX2I RGATCY 1 cut(s) 496
BstYI RGATCY 1 cut(s) 496
BsuI GTATCC 1 cut(s) 280
BtsCI GGATG 2 cut(s) 21, 58
BtsIMutI CAGTG 2 cut(s) 17, 441
Cac8I GCNNGC 2 cut(s) 259, 441
Csp6I GTAC 3 cut(s) 213, 269, 432
CviAII CATG 5 cut(s) 124, 233, 265, 334, 416
CviQI GTAC 3 cut(s) 213, 269, 432
DdeI CTNAG 5 cut(s) 68, 93, 195, 244, 483
DpnI GATC 3 cut(s) 74, 86, 498
DpnII GATC 3 cut(s) 72, 84, 496
Eam1104I CTCTTC 2 cut(s) 222, 366
EarI CTCTTC 2 cut(s) 222, 366
Ecl136II GAGCTC 1 cut(s) 92
Eco24I GRGCYC 1 cut(s) 94
Eco53kI GAGCTC 1 cut(s) 92
Eco57I CTGAAG 1 cut(s) 203
EcoICRI GAGCTC 1 cut(s) 92
EcoRI GAATTC 2 cut(s) 310, 411
EcoT38I GRGCYC 1 cut(s) 94
FaeI CATG 5 cut(s) 127, 236, 268, 337, 419
FaiI YATR 8 cut(s) 125, 234, 266, 335, 353, 393, 417, 505
FaqI GGGAC 1 cut(s) 355
FatI CATG 5 cut(s) 123, 232, 264, 333, 415
FauI CCCGC 1 cut(s) 432
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 2 cut(s) 28, 65
FriOI GRGCYC 1 cut(s) 94
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 1 cut(s) 258
GluI GCNGC 1 cut(s) 108
GsuI CTGGAG 1 cut(s) 144
HapII CCGG 1 cut(s) 34
Hin1II CATG 5 cut(s) 127, 236, 268, 337, 419
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 5 cut(s) 101, 133, 149, 240, 478
HpaII CCGG 1 cut(s) 34
HphI GGTGA 1 cut(s) 144
Hpy166II GTNNAC 1 cut(s) 178
Hpy188I TCNGA 4 cut(s) 89, 239, 247, 452
Hpy188III TCNNGA 2 cut(s) 70, 194
Hpy8I GTNNAC 1 cut(s) 178
Hpy99I CGWCG 3 cut(s) 44, 47, 62
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 2 cut(s) 107, 464
HpyF10VI GCNNNNNNNGC 1 cut(s) 205
HpyF3I CTNAG 5 cut(s) 68, 93, 195, 244, 483
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 5 cut(s) 127, 236, 268, 337, 419
Kzo9I GATC 3 cut(s) 72, 84, 496
LmnI GCTCC 3 cut(s) 36, 163, 254
Lsp1109I GCAGC 1 cut(s) 119
LweI GCATC 3 cut(s) 43, 270, 337
MaeI CTAG 1 cut(s) 258
MaeII ACGT 1 cut(s) 45
MalI GATC 3 cut(s) 74, 86, 498
MboI GATC 3 cut(s) 72, 84, 496
MboII GAAGA 5 cut(s) 209, 380, 383, 395, 506
MflI RGATCY 1 cut(s) 496
MhlI GDGCHC 1 cut(s) 94
MluCI AATT 2 cut(s) 310, 411
MlyI GAGTC 1 cut(s) 95
MnlI CCTC 3 cut(s) 244, 357, 367
MspI CCGG 1 cut(s) 34
MwoI GCNNNNNNNGC 1 cut(s) 205
NdeII GATC 3 cut(s) 72, 84, 496
NheI GCTAGC 1 cut(s) 257
NlaIII CATG 5 cut(s) 127, 236, 268, 337, 419
NspI RCATGY 1 cut(s) 236
PfeI GAWTC 4 cut(s) 133, 149, 240, 478
PkrI GCNGC 1 cut(s) 109
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
PsiI TTATAA 1 cut(s) 353
Psp124BI GAGCTC 1 cut(s) 94
PstI CTGCAG 1 cut(s) 109
PsuI RGATCY 1 cut(s) 496
RsaI GTAC 3 cut(s) 214, 270, 433
RsaNI GTAC 3 cut(s) 213, 269, 432
SacI GAGCTC 1 cut(s) 94
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 3 cut(s) 72, 84, 496
SchI GAGTC 1 cut(s) 95
SduI GDGCHC 1 cut(s) 94
SetI ASST 8 cut(s) 48, 69, 94, 160, 166, 201, 210, 259
SfaNI GCATC 3 cut(s) 43, 270, 337
SfcI CTRYAG 1 cut(s) 105
Sse9I AATT 2 cut(s) 310, 411
SsiI CCGC 1 cut(s) 439
SspI AATATT 1 cut(s) 296
SspMI CTAG 1 cut(s) 258
SstI GAGCTC 1 cut(s) 94
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 1 cut(s) 48
TaqI TCGA 2 cut(s) 99, 136
TasI AATT 2 cut(s) 310, 411
TfiI GAWTC 4 cut(s) 133, 149, 240, 478
TscAI CASTG 2 cut(s) 17, 441
TseI GCWGC 1 cut(s) 107
TspDTI ATGAA 4 cut(s) 140, 396, 404, 507
TspGWI ACGGA 1 cut(s) 423
TspRI CASTG 2 cut(s) 17, 441
XapI RAATTY 2 cut(s) 310, 411
XceI RCATGY 1 cut(s) 236
XspI CTAG 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.