Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0011654

Physical Location & Seq

Genomic Coordinates Reverse (-)

16790556 .. 16796193

Gene Structure 5638 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1185000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3048 bp

ATGGAGAGCTATTTGCAAGAGTTCGGGGAAGTCAAGGCGAAGGGCTCGTCGGAAGAAACGTTGCAGAAATGGAGGAACCTTTGCAGCGTTGTCAAGAACCCGAAACGGCGGTTTCGCTTCACTGCCAATCTTTCCAAGCGTTTCGAGGCCGCTGCTATGCGCCGTACCAATCAGGAGAAATTGAGGATTGCTGTTTTGGTTTCAAAAGCTGCATTTCAATTTATTCAAGGTGTGCAACCAAGTGACTACGTCGTACCCAAGGAAGTTACAGACGCAGGTTTTCATATTTGTGCAGATGAATTGGGATCCATTGTTGAAGGCCATGATGTAAAGAAGCTCAAATTTCATGGCGGGATAAGCGGCATCGCAGAAAAGATCTCCACATCTGTAAAAGATGGGCTCAACACCGAATCTGACATGCACACTCGAAGACAGGAGATTTATGGAATCAATAAATTTACGGAGAGTGAGCAACGGGGTTTCTTTATATTTGTTTGGGAAGCCCTTCAGGATATGACTCTTATGATCCTCGGAGTGTGCGCTTTCGTGTCTTTGATAGTTGGGATAGCAATGGAAGGGTGGCCGGTTGGAGCACATGATGGACTTGGAATCGTTGCGAGTATCCTGTTGGTTGTTCTTGTCACAGCAACAAGTGATTACCGCCAATCGCTGCAGTTCAAGGATTTGGACAAGGAGAAAAAGAAGATTGCTATGCAAGTAACAAGAAATGGATATAGGCAGAAAATGTCAATCTATGATTTACTGCCTGGTGATATTGTGCATCTTTCTATTGGAGACCAAGTGCCAGCAGATGGGCTATTCGTATCAGGATTCTCCGTGTTGATTGACGAATCAAGCTTAACTGGTGAGAGTGAGCCAATAATGGTAACTGCTGAAAACCCTTTTCTTCTTTCCGGCACTAAGGTCCAAGACGGATCATGCAAGATGATGGTTACAACAGTTGGGATGAGAACCCAATGGGGTAAATTGATGGCGACTCTGAGCGAAGGTGGAGATGATGAAACCCCATTGCAGGTGAAGTTGAATGGAGTGGCAACCATTATTGGAAAGATAGGACTTTTCTTCGCCGTCGTGACTTTTGCAGTTATGATGCAAGGATTGTTTAGTCGAAAACTGGCTGAGGGGACACACTGGAGCTGGAATGGAGATGATGCAAAGCAAATGTTGGAATTCTTTGCTATTGCAGTAACAATTGTTGTTGTTGCTGTCCCAGAGGGGCTGCCGCTGTCTGTGACATTGAGCCTTGCCTTTGCCATGAAGAAGATGATGAACGATAAAGCACTCGTTCGTCATCTCGCAGCTTGTGAGACTATGGGATCAGCCACAAACATTTGCAGTGACAAAACCGGGACACTAACAACCAACCGCATGACCGTTGTGAAATCATGCATTTGCATGAATGTCAAGGAGGTGAGTAAACCCAATGAGGCCTCTAGCTTGTTCTCTGATCTTCCGGAGTCTGCTAAGAAACTTTTGGTACAATCGATCTTTAACAACACTGGTGGAGAAATTGTGGTTAACAAAGCCGGAAAGCATGAGCTATTGGGAACACCTACAGATACTGCTTTGTTGGAATTTGGATTGTCAATTGGTGGAAATTTCCAAGCAGAGAGACAAGCTTCTAAACTTGTTAAAGTTGAGCCATTCAACTCAACGAAGAAACGGATGGGGGTAATCTTGGAGCTTCCTGAAGGAGGTTTGATAGCGCACACCAAAGGTGCCTCCGAAGTAGTTCTGGCCAGCTGTGAAAAGGTGATCAACTCAAATGGTGATATTGTTCCCCTTGATGAAGCATCCCTCAACCATCTCAAAGTTACTATTGAGCAGTTTGCTTGCGAGGCTCTTCGAACTCTATGTCTTGCGTATACAGAACTAGAAACTGGTTTCTCTCCTGAGAATTCCATTCCTGTTTCTGGTTATACCTGTATAGGAATTGTGGGAATTAAGGATCCTGTTCGTCCCGGTGTCAAGGAGTCTGTTGCAATCTGCCGTTCAGCGGGCATCACAGTAAGAATGGTCACCGGAGACAATATAAATACTGCAAAGGCTATCGCAAGGGAATGTGGGATTCTCACCGATGATGGCATAGCCATTGAAGGTCCGGAGTTCAGGGAGAAGAATCAGGAAGAATTGCTTTCTCTGATTCCTAAAATTCAGGTGATGGCTCGATCTTCACCTCTGGACAAGCATACACTGGTGAAGCACTTGCGGACCACATTTGATGAAGTTGTTGCTGTAACCGGTGATGGAACAAACGATGCCCCAGCGCTTCACGAGGCAGATATTGGACTAGCTATGGGGATTGCTGGAACTGAGGTGGCTAAAGAGAGTGCTGATGTTATAATTCTGGACGATAATTTCTCCACAATCGTGACAGTGGCAAAATGGGGACGCTCGGTTTACATCAACATTCAAAAATTTGTGCAGTTCCAGCTGACAGTCAATATAGTTGCATTGATTGTGAACTTCTCCTCGGCTTGCTTGACAGGGAGTGCTCCTCTCACAGCTGTGCAGCTATTGTGGGTCAACATGATCATGGACACGTTAGGCGCCCTTGCACTTGCAACTGAGCCTCCAAATAATGACCTAATGAAGCATCCGCCGGTTGGAAAGAGACAGAATTTTATCAACAATGTAATGTGGAGGAACATTTTAGGGCAGTCTTTGTATCAGTTTACGGTGATTTGGTTCCTTCAGGCAAAAGGAGAAGCAATGTTTGGTCTATACGGACCAGATTCTCATGTGATCCTCAACACTCTGATTTTCAACACATTTGTGTTCTGTCAGGTTTTCAACGAGATAAGCTCGCGCGATATGGAGGAAATAGATGTCTTCAAGGGCATATTGGATAACTATGTCTTCGTCGGAGTCCTCAGTTCCACAGTTGTGTTCCAAATCGTTATTATCGAGTTCCTGGGAAAATTTGCAAGCACAGCACCGCTCACCTTGGCACAATGGTTTGCCACTGTATTCATCGGATTTTTAGGGATGCCGATTGCTGCTGGTTTAAAGATGTTTCCTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007275 GO:0008150 GO:0008324 GO:0009555 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0031984 GO:0032501 GO:0032502 GO:0034220 GO:0042175 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048229 GO:0048856 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0098827 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1016

Amino Acids

110.66

Weight (kDa)

5.74

Isoelectric Point (pI)

34.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	6 - 49	2.2e-19	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	116 - 184	2.3e-09	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	237 - 333	1.8e-21	P-type ATPase actuator domain
Cation_ATPase	PF13246	503 - 661	4.2e-33	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	648 - 768	1.8e-11	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	839 - 1013	2.5e-42	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Image

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Orthologous Genes (Group: OG0011654)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22950 AT4G37640
fragaria_vesca	FvH4_1g17620
malus_domestica	MD02G1185000.v1.1 MD15G1296400.v1.1
prunus_persica	Prupe.6G221800_v2.0.a1 Prupe.6G221800_v2.0.a1
pyrus_communis	pycom02g14990 pycom15g25970
rosa_chinensis	RchiOBHm_Chr2g0107911
rosa_laevigata	RLG00000017627
rosa_multiflora	Rmu_sc0001484.1_g000032
rosa_roxburghii	Rroxscaffold_2G00135800
rosa_rugosa	Rorug02G0148700 Rorug02G0148800 Rorug02G0148800
rosa_samantha	Rh2BG210900 Rh2CG202700
rosa_wichuraiana	Rw2G015710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2363
AarI	CACCTGC	1 cut(s)	1024
Acc36I	ACCTGC	2 cut(s)	266, 1024
AccB1I	GGYRCC	2 cut(s)	1739, 2570
AccB7I	CCANNNNNTGG	1 cut(s)	812
AccBSI	CCGCTC	1 cut(s)	2962
AccI	GTMKAC	1 cut(s)	1886
AccII	CGCG	2 cut(s)	2830, 2832
AccIII	TCCGGA	2 cut(s)	1474, 2122
AclI	AACGTT	1 cut(s)	59
AclWI	GGATC	8 cut(s)	300, 313, 520, 943, 1345, 1964, 1977, 2761
AcoI	YGGCCR	2 cut(s)	581, 1758
AcuI	CTGAAG	3 cut(s)	491, 1731, 2699
AcyI	GRCGYC	1 cut(s)	2571
AfaI	GTAC	3 cut(s)	166, 255, 1500
AfeI	AGCGCT	1 cut(s)	2289
AfiI	CCNNNNNNNGG	6 cut(s)	812, 1033, 1715, 1934, 2017, 2627
AflIII	ACRYGT	1 cut(s)	2562
AgeI	ACCGGT	1 cut(s)	2261
AjnI	CCWGG	2 cut(s)	766, 2934
AjuI	GAANNNNNNNTTGG	2 cut(s)	2721, 2753
AleI	CACNNNNGTG	4 cut(s)	2390, 2528, 2795, 2906
Alw21I	GWGCWC	2 cut(s)	595, 2518
Alw26I	GTCTC	5 cut(s)	789, 1322, 1627, 2040, 2629
AlwI	GGATC	8 cut(s)	300, 313, 520, 943, 1345, 1964, 1977, 2761
AlwNI	CAGNNNCTG	1 cut(s)	1583
Aor13HI	TCCGGA	2 cut(s)	1474, 2122
Aor51HI	AGCGCT	1 cut(s)	2289
AoxI	GGCC	5 cut(s)	147, 319, 581, 1449, 1758
ApeKI	GCWGC	8 cut(s)	84, 152, 209, 670, 1240, 1319, 2533, 3020
ArsI	GACNNNNNNTTYG	4 cut(s)	32, 64, 1861, 1893
AsiGI	ACCGGT	1 cut(s)	2261
Asp700I	GAANNNNTTC	2 cut(s)	504, 1752
AspLEI	GCGC	6 cut(s)	162, 542, 1729, 2290, 2573, 2832
AspS9I	GGNCC	4 cut(s)	925, 2120, 2232, 2750
AsuC2I	CCSGG	2 cut(s)	1369, 1983
AsuII	TTCGAA	1 cut(s)	1867
AvaII	GGWCC	4 cut(s)	925, 2120, 2232, 2750
BalI	TGGCCA	1 cut(s)	1760
BamHI	GGATCC	2 cut(s)	305, 1969
BanI	GGYRCC	2 cut(s)	1739, 2570
BanII	GRGCYC	2 cut(s)	47, 402
BauI	CACGAG	1 cut(s)	2294
BbsI	GAAGAC	3 cut(s)	436, 2845, 2872
Bbv12I	GWGCWC	2 cut(s)	595, 2518
BbvCI	CCTCAGC	1 cut(s)	1140
BbvI	GCAGC	8 cut(s)	96, 139, 196, 657, 1227, 1331, 2545, 3007
BceAI	ACGGC	4 cut(s)	122, 147, 1073, 1995
BcgI	CGANNNNNNTGC	2 cut(s)	134, 168
BciT130I	CCWGG	2 cut(s)	768, 2936
BciVI	GTATCC	1 cut(s)	632
BclI	TGATCA	2 cut(s)	1776, 2553
BcnI	CCSGG	2 cut(s)	1369, 1983
BcoDI	GTCTC	5 cut(s)	789, 1322, 1627, 2040, 2629
BfaI	CTAG	3 cut(s)	1455, 1895, 2312
BfmI	CTRYAG	2 cut(s)	671, 1575
BfoI	RGCGCY	2 cut(s)	2291, 2574
BfuAI	ACCTGC	2 cut(s)	266, 1024
BfuI	GTATCC	1 cut(s)	632
BglII	AGATCT	1 cut(s)	375
Bme1390I	CCNGG	4 cut(s)	768, 1369, 1983, 2936
Bme18I	GGWCC	4 cut(s)	925, 2120, 2232, 2750
BmgT120I	GGNCC	4 cut(s)	925, 2120, 2232, 2750
BmiI	GGNNCC	6 cut(s)	77, 307, 1741, 1971, 2572, 2711
BmrFI	CCNGG	4 cut(s)	768, 1369, 1983, 2936
BmsI	GCATC	9 cut(s)	372, 790, 1101, 1162, 1823, 2031, 2269, 2626, 3000
BpiI	GAAGAC	3 cut(s)	436, 2845, 2872
BplI	GAGNNNNNCTC	4 cut(s)	2503, 2535, 2810, 2842
BpmI	CTGGAG	1 cut(s)	1174
Bpu10I	CCTNAGC	1 cut(s)	1140
Bpu14I	TTCGAA	1 cut(s)	1867
BpuMI	CCSGG	2 cut(s)	1369, 1983
Bsa29I	ATCGAT	1 cut(s)	1505
BsaHI	GRCGYC	1 cut(s)	2571
BsaI	GGTCTC	1 cut(s)	789
BsaJI	CCNNGG	5 cut(s)	258, 529, 2493, 2935, 2967
BsaWI	WCCGGW	4 cut(s)	1474, 2042, 2122, 2261
BsaXI	ACNNNNNCTCC	6 cut(s)	786, 816, 1518, 1548, 2578, 2608
Bsc4I	CCNNNNNNNGG	6 cut(s)	812, 1033, 1715, 1934, 2017, 2627
Bse118I	RCCGGY	3 cut(s)	583, 2261, 2623
Bse1I	ACTGG	6 cut(s)	868, 1140, 1157, 1525, 1906, 2220
Bse3DI	GCAATG	3 cut(s)	576, 1028, 2739
BseAI	TCCGGA	2 cut(s)	1474, 2122
BseBI	CCWGG	2 cut(s)	768, 2936
BseCI	ATCGAT	1 cut(s)	1505
BseDI	CCNNGG	5 cut(s)	258, 529, 2493, 2935, 2967
BseGI	GGATG	5 cut(s)	972, 1692, 1814, 2617, 3015
BseLI	CCNNNNNNNGG	6 cut(s)	812, 1033, 1715, 1934, 2017, 2627
BseMI	GCAATG	3 cut(s)	576, 1028, 2739
BseMII	CTCAG	6 cut(s)	992, 1131, 1905, 2325, 2580, 2908
BseNI	ACTGG	6 cut(s)	868, 1140, 1157, 1525, 1906, 2220
BseRI	GAGGAG	2 cut(s)	2482, 2508
BseXI	GCAGC	8 cut(s)	96, 139, 196, 657, 1227, 1331, 2545, 3007
BseYI	CCCAGC	1 cut(s)	2284
BsgI	GTGCAG	3 cut(s)	312, 2465, 2552
Bsh1236I	CGCG	2 cut(s)	2830, 2832
BshFI	GGCC	5 cut(s)	149, 321, 583, 1451, 1760
BshNI	GGYRCC	2 cut(s)	1739, 2570
BshTI	ACCGGT	1 cut(s)	2261
BshVI	ATCGAT	1 cut(s)	1505
BsiHKAI	GWGCWC	2 cut(s)	595, 2518
BslFI	GGGAC	5 cut(s)	1159, 1214, 1384, 1965, 2424
BslI	CCNNNNNNNGG	6 cut(s)	812, 1033, 1715, 1934, 2017, 2627
BsmAI	GTCTC	5 cut(s)	789, 1322, 1627, 2040, 2629
BsmFI	GGGAC	5 cut(s)	1159, 1214, 1384, 1965, 2424
BsnI	GGCC	5 cut(s)	149, 321, 583, 1451, 1760
Bso31I	GGTCTC	1 cut(s)	789
Bsp119I	TTCGAA	1 cut(s)	1867
Bsp1286I	GDGCHC	4 cut(s)	47, 402, 595, 2518
Bsp13I	TCCGGA	2 cut(s)	1474, 2122
BspANI	GGCC	5 cut(s)	149, 321, 583, 1451, 1760
BspCNI	CTCAG	6 cut(s)	993, 1132, 1906, 2326, 2581, 2907
BspDI	ATCGAT	1 cut(s)	1505
BspEI	TCCGGA	2 cut(s)	1474, 2122
BspFNI	CGCG	2 cut(s)	2830, 2832
BspLI	GGNNCC	6 cut(s)	77, 307, 1741, 1971, 2572, 2711
BspMAI	CTGCAG	1 cut(s)	675
BspMI	ACCTGC	2 cut(s)	266, 1024
BspPI	GGATC	8 cut(s)	300, 313, 520, 943, 1345, 1964, 1977, 2761
BspQI	GCTCTTC	1 cut(s)	1869
BspT104I	TTCGAA	1 cut(s)	1867
BspT107I	GGYRCC	2 cut(s)	1739, 2570
BspTNI	GGTCTC	1 cut(s)	789
BsrBI	CCGCTC	1 cut(s)	2962
BsrDI	GCAATG	3 cut(s)	576, 1028, 2739
BsrFI	RCCGGY	3 cut(s)	583, 2261, 2623
BsrI	ACTGG	6 cut(s)	868, 1140, 1157, 1525, 1906, 2220
BssAI	RCCGGY	3 cut(s)	583, 2261, 2623
BssECI	CCNNGG	5 cut(s)	258, 529, 2493, 2935, 2967
BssNAI	GTATAC	1 cut(s)	1887
BssNI	GRCGYC	1 cut(s)	2571
BssSI	CACGAG	1 cut(s)	2294
BssT1I	CCWWGG	2 cut(s)	258, 2967
Bst1107I	GTATAC	1 cut(s)	1887
Bst2BI	CACGAG	1 cut(s)	2294
Bst2UI	CCWGG	2 cut(s)	768, 2936
Bst4CI	ACNGT	8 cut(s)	961, 1396, 2029, 2398, 2461, 2701, 2905, 2989
Bst6I	CTCTTC	1 cut(s)	1869
BstACI	GRCGYC	1 cut(s)	2571
BstBI	TTCGAA	1 cut(s)	1867
BstC8I	GCNNGC	7 cut(s)	807, 1762, 1855, 2020, 2500, 2828, 2950
BstDEI	CTNAG	8 cut(s)	921, 1001, 1140, 1485, 1914, 2334, 2589, 2894
BstEII	GGTNACC	1 cut(s)	2038
BstENI	CCTNNNNNAGG	1 cut(s)	1713
BstF5I	GGATG	5 cut(s)	972, 1692, 1814, 2617, 3015
BstFNI	CGCG	2 cut(s)	2830, 2832
BstH2I	RGCGCY	2 cut(s)	2291, 2574
BstHHI	GCGC	6 cut(s)	162, 542, 1729, 2290, 2573, 2832
BstMAI	GTCTC	5 cut(s)	789, 1322, 1627, 2040, 2629
BstMWI	GCNNNNNNNGC	4 cut(s)	357, 1859, 2452, 2954
BstNI	CCWGG	2 cut(s)	768, 2936
BstNSI	RCATGY	1 cut(s)	421
BstPI	GGTNACC	1 cut(s)	2038
BstSCI	CCNGG	4 cut(s)	766, 1367, 1981, 2934
BstSFI	CTRYAG	2 cut(s)	671, 1575
BstUI	CGCG	2 cut(s)	2830, 2832
BstV1I	GCAGC	8 cut(s)	96, 139, 196, 657, 1227, 1331, 2545, 3007
BstV2I	GAAGAC	3 cut(s)	436, 2845, 2872
BstX2I	RGATCY	3 cut(s)	305, 375, 1969
BstYI	RGATCY	3 cut(s)	305, 375, 1969
BstZ17I	GTATAC	1 cut(s)	1887
Bsu15I	ATCGAT	1 cut(s)	1505
BsuI	GTATCC	1 cut(s)	632
BsuRI	GGCC	5 cut(s)	149, 321, 583, 1451, 1760
BsuTUI	ATCGAT	1 cut(s)	1505
BtgZI	GCGATG	1 cut(s)	349
BtsCI	GGATG	5 cut(s)	972, 1692, 1814, 2617, 3015
BtsI	GCAGTG	2 cut(s)	120, 1363
BtsIMutI	CAGTG	7 cut(s)	120, 1150, 1363, 1518, 2213, 2403, 2985
BveI	ACCTGC	2 cut(s)	266, 1024
Cac8I	GCNNGC	7 cut(s)	807, 1762, 1855, 2020, 2500, 2828, 2950
CaiI	CAGNNNCTG	1 cut(s)	1583
CfoI	GCGC	6 cut(s)	162, 542, 1729, 2290, 2573, 2832
Cfr10I	RCCGGY	3 cut(s)	583, 2261, 2623
Cfr13I	GGNCC	4 cut(s)	925, 2120, 2232, 2750
ClaI	ATCGAT	1 cut(s)	1505
CseI	GACGC	2 cut(s)	281, 2421
Csp6I	GTAC	3 cut(s)	165, 254, 1499
CspAI	ACCGGT	1 cut(s)	2261
CspCI	CAANNNNNGTGG	2 cut(s)	1504, 1539
CviQI	GTAC	3 cut(s)	165, 254, 1499
DdeI	CTNAG	8 cut(s)	921, 1001, 1140, 1485, 1914, 2334, 2589, 2894
DinI	GGCGCC	1 cut(s)	2572
DraI	TTTAAA	1 cut(s)	3030
EaeI	YGGCCR	2 cut(s)	581, 1758
Eam1104I	CTCTTC	1 cut(s)	1869
EarI	CTCTTC	1 cut(s)	1869
EciI	GGCGGA	1 cut(s)	2610
Eco130I	CCWWGG	2 cut(s)	258, 2967
Eco147I	AGGCCT	1 cut(s)	1451
Eco24I	GRGCYC	2 cut(s)	47, 402
Eco31I	GGTCTC	1 cut(s)	789
Eco47I	GGWCC	4 cut(s)	925, 2120, 2232, 2750
Eco47III	AGCGCT	1 cut(s)	2289
Eco57I	CTGAAG	3 cut(s)	491, 1731, 2699
Eco91I	GGTNACC	1 cut(s)	2038
EcoNI	CCTNNNNNAGG	1 cut(s)	1713
EcoO65I	GGTNACC	1 cut(s)	2038
EcoRI	GAATTC	2 cut(s)	1190, 1918
EcoRII	CCWGG	2 cut(s)	766, 2934
EcoT14I	CCWWGG	2 cut(s)	258, 2967
EcoT22I	ATGCAT	1 cut(s)	1412
EcoT38I	GRGCYC	2 cut(s)	47, 402
EgeI	GGCGCC	1 cut(s)	2572
EheI	GGCGCC	1 cut(s)	2572
ErhI	CCWWGG	2 cut(s)	258, 2967
FalI	AAGNNNNNCTT	2 cut(s)	2139, 2171
FaqI	GGGAC	5 cut(s)	1159, 1214, 1384, 1965, 2424
FauI	CCCGC	2 cut(s)	344, 2011
FbaI	TGATCA	2 cut(s)	1776, 2553
FblI	GTMKAC	1 cut(s)	1886
FokI	GGATG	5 cut(s)	979, 1699, 1801, 2604, 3022
FriOI	GRGCYC	2 cut(s)	47, 402
FspBI	CTAG	3 cut(s)	1455, 1895, 2312
GlaI	GCGC	6 cut(s)	161, 541, 1728, 2289, 2572, 2831
GsaI	CCCAGC	1 cut(s)	2288
GsuI	CTGGAG	1 cut(s)	1174
HaeII	RGCGCY	2 cut(s)	2291, 2574
HaeIII	GGCC	5 cut(s)	149, 321, 583, 1451, 1760
HgaI	GACGC	2 cut(s)	281, 2421
HhaI	GCGC	6 cut(s)	162, 542, 1729, 2290, 2573, 2832
Hin1I	GRCGYC	1 cut(s)	2571
Hin6I	GCGC	6 cut(s)	160, 540, 1727, 2288, 2571, 2830
HinP1I	GCGC	6 cut(s)	160, 540, 1727, 2288, 2571, 2830
HincII	GTYRAC	2 cut(s)	1540, 2548
HindII	GTYRAC	2 cut(s)	1540, 2548
HindIII	AAGCTT	2 cut(s)	856, 1638
HpaI	GTTAAC	1 cut(s)	1540
Hpy166II	GTNNAC	7 cut(s)	1439, 1540, 1887, 2422, 2485, 2548, 2697
Hpy8I	GTNNAC	7 cut(s)	1439, 1540, 1887, 2422, 2485, 2548, 2697
Hpy99I	CGWCG	4 cut(s)	52, 254, 1094, 2888
HpyAV	CCTTC	8 cut(s)	34, 311, 515, 569, 1001, 1706, 2111, 2723
HpyCH4III	ACNGT	8 cut(s)	961, 1396, 2029, 2398, 2461, 2701, 2905, 2989
HpyCH4IV	ACGT	3 cut(s)	59, 249, 2564
HpyF10VI	GCNNNNNNNGC	4 cut(s)	357, 1859, 2452, 2954
HpyF3I	CTNAG	8 cut(s)	921, 1001, 1140, 1485, 1914, 2334, 2589, 2894
HpySE526I	ACGT	3 cut(s)	59, 249, 2564
Hsp92I	GRCGYC	1 cut(s)	2571
HspAI	GCGC	6 cut(s)	160, 540, 1727, 2288, 2571, 2830
KasI	GGCGCC	1 cut(s)	2570
Kpn2I	TCCGGA	2 cut(s)	1474, 2122
Ksp22I	TGATCA	2 cut(s)	1776, 2553
KspAI	GTTAAC	1 cut(s)	1540
LguI	GCTCTTC	1 cut(s)	1869
LmnI	GCTCC	4 cut(s)	590, 1155, 1702, 2521
Lsp1109I	GCAGC	8 cut(s)	96, 139, 196, 657, 1227, 1331, 2545, 3007
LweI	GCATC	9 cut(s)	372, 790, 1101, 1162, 1823, 2031, 2269, 2626, 3000
MaeI	CTAG	3 cut(s)	1455, 1895, 2312
MaeII	ACGT	3 cut(s)	59, 249, 2564
MbiI	CCGCTC	1 cut(s)	2962
MfeI	CAATTG	2 cut(s)	1212, 1608
MflI	RGATCY	3 cut(s)	305, 375, 1969
MhlI	GDGCHC	4 cut(s)	47, 402, 595, 2518
MlsI	TGGCCA	1 cut(s)	1760
MluNI	TGGCCA	1 cut(s)	1760
Mly113I	GGCGCC	1 cut(s)	2571
MlyI	GAGTC	5 cut(s)	511, 991, 1487, 2003, 2898
MmeI	TCCRAC	6 cut(s)	30, 568, 1167, 1572, 2608, 2866
Mox20I	TGGCCA	1 cut(s)	1760
Mph1103I	ATGCAT	1 cut(s)	1412
MroI	TCCGGA	2 cut(s)	1474, 2122
MroXI	GAANNNNTTC	2 cut(s)	504, 1752
MscI	TGGCCA	1 cut(s)	1760
MseI	TTAA	6 cut(s)	860, 1512, 1539, 1653, 1965, 3029
MslI	CAYNNNNRTG	7 cut(s)	288, 1415, 2390, 2528, 2555, 2795, 2906
Msp20I	TGGCCA	1 cut(s)	1760
MspA1I	CMGCKG	6 cut(s)	152, 1246, 1764, 2018, 2455, 2528
MspR9I	CCNGG	4 cut(s)	768, 1369, 1983, 2936
MunI	CAATTG	2 cut(s)	1212, 1608
MvaI	CCWGG	2 cut(s)	768, 2936
MvnI	CGCG	2 cut(s)	2830, 2832
MwoI	GCNNNNNNNGC	4 cut(s)	357, 1859, 2452, 2954
NarI	GGCGCC	1 cut(s)	2571
NciI	CCSGG	2 cut(s)	1369, 1983
NlaIV	GGNNCC	6 cut(s)	77, 307, 1741, 1971, 2572, 2711
NmeAIII	GCCGAG	1 cut(s)	2474
NmuCI	GTSAC	7 cut(s)	242, 640, 1093, 1252, 1358, 2038, 2392
NsiI	ATGCAT	1 cut(s)	1412
NspI	RCATGY	1 cut(s)	421
NspV	TTCGAA	1 cut(s)	1867
OliI	CACNNNNGTG	4 cut(s)	2390, 2528, 2795, 2906
PaqCI	CACCTGC	1 cut(s)	1024
PceI	AGGCCT	1 cut(s)	1451
PciSI	GCTCTTC	1 cut(s)	1869
PcsI	WCGNNNNNNNCGW	2 cut(s)	56, 2925
PdmI	GAANNNNTTC	2 cut(s)	504, 1752
PfeI	GAWTC	9 cut(s)	410, 447, 609, 831, 851, 2089, 2140, 2164, 2756
PflFI	GACNNNGTC	1 cut(s)	248
PflMI	CCANNNNNTGG	1 cut(s)	812
PinAI	ACCGGT	1 cut(s)	2261
PleI	GAGTC	5 cut(s)	511, 991, 1486, 2002, 2897
PluTI	GGCGCC	1 cut(s)	2574
PpsI	GAGTC	5 cut(s)	511, 991, 1486, 2002, 2897
PsiI	TTATAA	1 cut(s)	2363
Psp1406I	AACGTT	1 cut(s)	59
Psp6I	CCWGG	2 cut(s)	766, 2934
PspEI	GGTNACC	1 cut(s)	2038
PspFI	CCCAGC	1 cut(s)	2284
PspGI	CCWGG	2 cut(s)	766, 2934
PspN4I	GGNNCC	6 cut(s)	77, 307, 1741, 1971, 2572, 2711
PspPI	GGNCC	4 cut(s)	925, 2120, 2232, 2750
PstI	CTGCAG	1 cut(s)	675
PstNI	CAGNNNCTG	1 cut(s)	1583
PsuI	RGATCY	3 cut(s)	305, 375, 1969
PsyI	GACNNNGTC	1 cut(s)	248
PvuII	CAGCTG	3 cut(s)	1764, 2455, 2528
RsaI	GTAC	3 cut(s)	166, 255, 1500
RsaNI	GTAC	3 cut(s)	165, 254, 1499
RseI	CAYNNNNRTG	7 cut(s)	288, 1415, 2390, 2528, 2555, 2795, 2906
SapI	GCTCTTC	1 cut(s)	1869
SaqAI	TTAA	6 cut(s)	860, 1512, 1539, 1653, 1965, 3029
Sau96I	GGNCC	4 cut(s)	925, 2120, 2232, 2750
SchI	GAGTC	5 cut(s)	511, 991, 1487, 2003, 2898
ScrFI	CCNGG	4 cut(s)	768, 1369, 1983, 2936
SduI	GDGCHC	4 cut(s)	47, 402, 595, 2518
SfaNI	GCATC	9 cut(s)	372, 790, 1101, 1162, 1823, 2031, 2269, 2626, 3000
SfcI	CTRYAG	2 cut(s)	671, 1575
SfoI	GGCGCC	1 cut(s)	2572
SfuI	TTCGAA	1 cut(s)	1867
SinI	GGWCC	4 cut(s)	925, 2120, 2232, 2750
SmiMI	CAYNNNNRTG	7 cut(s)	288, 1415, 2390, 2528, 2555, 2795, 2906
SseBI	AGGCCT	1 cut(s)	1451
SspDI	GGCGCC	1 cut(s)	2570
SspMI	CTAG	3 cut(s)	1455, 1895, 2312
StuI	AGGCCT	1 cut(s)	1451
StyD4I	CCNGG	4 cut(s)	766, 1367, 1981, 2934
StyI	CCWWGG	2 cut(s)	258, 2967
TaaI	ACNGT	8 cut(s)	961, 1396, 2029, 2398, 2461, 2701, 2905, 2989
TaiI	ACGT	3 cut(s)	62, 252, 2567
TaqI	TCGA	7 cut(s)	144, 427, 1129, 1505, 1867, 2188, 2928
TauI	GCSGC	3 cut(s)	152, 363, 1246
TfiI	GAWTC	9 cut(s)	410, 447, 609, 831, 851, 2089, 2140, 2164, 2756
Tru1I	TTAA	6 cut(s)	860, 1512, 1539, 1653, 1965, 3029
Tru9I	TTAA	6 cut(s)	860, 1512, 1539, 1653, 1965, 3029
TscAI	CASTG	7 cut(s)	127, 1157, 1363, 1525, 2220, 2403, 2992
TseFI	GTSAC	7 cut(s)	242, 640, 1093, 1252, 1358, 2038, 2392
TseI	GCWGC	8 cut(s)	84, 152, 209, 670, 1240, 1319, 2533, 3020
Tsp45I	GTSAC	7 cut(s)	242, 640, 1093, 1252, 1358, 2038, 2392
TspGWI	ACGGA	5 cut(s)	476, 826, 948, 1699, 2763
TspRI	CASTG	7 cut(s)	127, 1157, 1363, 1525, 2220, 2403, 2992
Tth111I	GACNNNGTC	1 cut(s)	248
Van91I	CCANNNNNTGG	1 cut(s)	812
VpaK11BI	GGWCC	4 cut(s)	925, 2120, 2232, 2750
XagI	CCTNNNNNAGG	1 cut(s)	1713
XceI	RCATGY	1 cut(s)	421
XmiI	GTMKAC	1 cut(s)	1886
XmnI	GAANNNNTTC	2 cut(s)	504, 1752
XspI	CTAG	3 cut(s)	1455, 1895, 2312
Zsp2I	ATGCAT	1 cut(s)	1412

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.