Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011654

Physical Location & Seq

Genomic Coordinates Reverse (-)

27800987 .. 27805458

Gene Structure 4472 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1296400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3048 bp

ATGGAGAGCTATTTGCAAGAGTTCGGGGAAGTGAAGCCGAAGGGTTCGTCTGAAGAAACGTTGCAGAAATGGAGGAACCTTTGCATCGTTGTCAAGAATCCGAAACGGCGGTTTCGCTTCACTGCCAATCTTTCCAAGCGTTTCGAGGCTGCTGCTATGCGCCGTACCAATCAGGAGAAACTGAGGATTGCTGTTTTGGTTTCTAAAGCTGCATTTCAATTTATTCAAGGTGTGCAACCAAGCGACTACGTCGTACCCAAGGAAGTTACAGACGCAGGTTTTCAGATTTGTGCTGATGAACTGGGATCCATTGTTGAAGGCCACGATGTAAAGAAGCTAAAATTTCATGGCGGAGTAAATGGCATCGCAGAAAAGATCTCCACATCAGTAAAAGATGGGCTCAACACCGAATCAGACTTGCACACTCGAAGACAGGAGATTTATGGAATCAATAAATTTACCGAGAGTGAGCAACGGGGTTTCTTGGTATTTGTTTGGGAAGCCCTTCAGGATATGACTCTTATGATCCTCGGAGTGTGCGCATTCGTGTCTCTGGTAGTTGGGATAGCAATGGAAGGGTGGCCAGTTGGAGCACATGATGGACTTGGAATCGTTGCGAGTATCCTGTTGGTTGTTCTTGTCACAGCAACAAGTGATTACCGCCAATCGTTGCAGTTCAAGGACTTGGACAAGGAGAAAAAGAAGATTGCGATGCAAGTAACAAGAAATGGATATAGGCAGAAAATGTCAATCTATGATTTACTGCCTGGCGATATTGTGCATCTTTCTATTGGAGACCAAGTGCCAGCAGATGGGCTCTTCGTATCAGGATTTTCTGTGTTGATTGACGAATCAAGCTTAACTGGTGAGAGTGAGCCAATAATGGTAACTCCTGAAAACCCTTTTCTTCTTTCGGGGACTAAGGTCCAAGACGGGTCATGCAAGATGATGGTTACAACGGTTGGGATGAGAACCCAGTGGGGTAAACTGATGGCGACTCTGAGCGAAGGCGGAGATGATGAAACCCCCCTGCAGGTGAAGCTGAATGGAGTGGCAACCATTATTGGAAAGATAGGCCTTTTCTTCGCCGTTGTGACTTTTGCAGTTATGGTGCAAGGATTGTTTAGTCGTAAACTGGCTGAGGGAACACACTGGAGCTGGAATGGAGATGATGCAATGCAGATGCTGGAATTCTTTGCTGTTGCAGTTACAATTGTTGTTGTTGCTGTCCCTGAGGGGCTGCCGCTGGCTGTGACATTGAGCCTTGCCTTTGCCATGAAGAAGATGATGAATGATAAAGCACTCGTTCGTCATCTGGCAGCTTGCGAGACTATGGGATCAGCCACAAATATTTGCAGTGACAAAACCGGGACACTAACAACCAACCGCATGACCGTTGTAAAATCATGCATTTGCATGAATGTCAAGGAGGTGAGCAATCCCAATGAGGCCTCTAGGTTATTCTCTGACCTTCCGGAATCTGCTAAGAAACTTTTGCTACAATCAATCTTTAACAACACCGGCGGAGAAGTTGTGGTTAACAAAGCTGGAAAGTATGAGCTGTTGGGAACACCTACAGATACTGCTTTGTTGGAACTTGGCTTGTCACTAGGTGGAAATTTCCAAGCAGAGAGACAAGCTTCTAAGCTTGTTAAAGTCGAGCCATTCAACTCAACGAAGAAACGGATGGGGGTAATCTTGGAGCTTCCCGAAGGAGGTCTGAGAGCGCACACCAAAGGTGCCTCAGAAATAGTTTTGGCCAGCTGCGAAAAGGTGATCAACTCAAATGGCGAAGTTGTTCCCCTTGATAAAGCATCCCTCAACCATCTCAAAGTTACTATTGAGCAATTTGCTTGCGAGGCTCTTCGAACTCTATGTCTTGCGTATACAGAACTAGAAACTGGTTTCTCTCCAGAGAATCCCATTCCTGTTTCTGGTTTTACTTGTATAGGAATTGTGGGAATTAAGGATCCTGTTCGTCCCGGTGTCAAAGAGTCAGTTGCAATTTGCCGTTCAGCTGGCATTACAGTAAGAATGGTTACTGGAGACAATATAAATACTGCAAAGGCTATCGCAAGAGAATGTGGTATTCTCACCGATGATGGCATAGCCATTGAAGGTCCGGAGTTCAGGGAGAAGAATCAGGAAGAATTGCTTTCTCTGATTCCTAAACTTCAGGTGATGGCTCGATCTTCACCTCTTGACAAGCATACATTGGTGCAGCACTTGCGGACCACATTTGATGAAGTTGTTGCTGTCACCGGTGATGGAACAAACGATGCCCCAGCGCTTCACGAGGCAGATATTGGACTAGCCATGGGGATTGCTGGAACTGAGGTGGCCAAAGAGAGTGCCGATGTTATAATTCTGGATGATAATTTCTCCACCATCGTAACAGTGGCGAAATGGGGACGTTCGGTTTACATCAACATTCAAAAATTTGTGCAATTCCAGCTGACGGTTAATATAGTTGCATTGATCGTGAATTTCTCCTCTGCTTGCTTGACAGGGAGCGCTCCTCTCACAGCTGTGCAGCTATTGTGGGTTAACATGATCATGGACACATTAGGCGCCCTTGCTCTTGCCACCGAGCCTCCGAATAATGACCTAATGAAGCGTCCGCCGGTTGGAAAGAGACAGAATTTTATCAACAATGTAATGTGGAGGAACATTCTGGGGCAGTCTTTCTATCAGTTTACCATGATTTGGTTCCTTCAGGCAAAAGGAGAAGCAATGTTTGGTCTATACGGACCGGACTCTCATGTGATCCTCAACACACTGATTTTCAACACATTTGTGTTCTGTCAGATTTTCAACGAAATAAGCTCGCGCGAGATGGAGGAAATAGATGTCTTCAAGGGCATACTGGATAACTATGTCTTCGTCGCAGTCCTCGGTTTCACAGTTGTGTTCCAAATCATTATTATCGAGTTCCTGGGAAAATTTGCAAGCACAGCACCCCTCACCCTGGCACAATGGTTCGTCACTGTATTCATCGGGTTTTTAGGGATGCCGATTGCTGCCGGATTAAAGATGTTTCCCGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007275 GO:0008150 GO:0008324 GO:0009555 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0031984 GO:0032501 GO:0032502 GO:0034220 GO:0042175 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048229 GO:0048856 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0098827 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1016

Amino Acids

110.9

Weight (kDa)

5.75

Isoelectric Point (pI)

35.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	6 - 49	1.5e-18	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	116 - 184	5.5e-10	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	237 - 333	2.4e-21	P-type ATPase actuator domain
Cation_ATPase	PF13246	498 - 661	3.3e-33	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	648 - 768	1.8e-11	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	839 - 1013	7.9e-43	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011654)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22950 AT4G37640
fragaria_vesca	FvH4_1g17620
malus_domestica	MD02G1185000.v1.1 MD15G1296400.v1.1
prunus_persica	Prupe.6G221800_v2.0.a1 Prupe.6G221800_v2.0.a1
pyrus_communis	pycom02g14990 pycom15g25970
rosa_chinensis	RchiOBHm_Chr2g0107911
rosa_laevigata	RLG00000017627
rosa_multiflora	Rmu_sc0001484.1_g000032
rosa_roxburghii	Rroxscaffold_2G00135800
rosa_rugosa	Rorug02G0148700 Rorug02G0148800 Rorug02G0148800
rosa_samantha	Rh2BG210900 Rh2CG202700
rosa_wichuraiana	Rw2G015710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2363
AarI	CACCTGC	1 cut(s)	1024
Acc16I	TGCGCA	1 cut(s)	541
Acc36I	ACCTGC	2 cut(s)	266, 1024
AccB1I	GGYRCC	2 cut(s)	1739, 2570
AccB7I	CCANNNNNTGG	2 cut(s)	812, 2706
AccI	GTMKAC	1 cut(s)	1886
AccII	CGCG	2 cut(s)	2830, 2832
AccIII	TCCGGA	2 cut(s)	1474, 2122
AciI	CCGC	9 cut(s)	109, 351, 661, 1011, 1244, 1387, 1524, 2230, 2621
AclI	AACGTT	1 cut(s)	59
AclWI	GGATC	7 cut(s)	300, 313, 520, 1345, 1964, 1977, 2761
AcoI	YGGCCR	3 cut(s)	581, 1758, 2340
AcsI	RAATTY	8 cut(s)	341, 455, 1190, 1618, 2438, 2485, 2641, 2942
AcuI	CTGAAG	4 cut(s)	72, 491, 2159, 2699
AcyI	GRCGYC	1 cut(s)	2571
AdeI	CACNNNGTG	1 cut(s)	1613
AfaI	GTAC	2 cut(s)	166, 255
AfeI	AGCGCT	2 cut(s)	2289, 2515
AfiI	CCNNNNNNNGG	8 cut(s)	812, 1033, 1715, 1934, 2458, 2627, 2706, 2968
AgeI	ACCGGT	1 cut(s)	2261
AjnI	CCWGG	3 cut(s)	766, 2934, 2967
AjuI	GAANNNNNNNTTGG	2 cut(s)	2721, 2753
AleI	CACNNNNGTG	3 cut(s)	2528, 2795, 2906
Alw21I	GWGCWC	1 cut(s)	595
Alw26I	GTCTC	6 cut(s)	555, 789, 1322, 1627, 2040, 2629
AlwI	GGATC	7 cut(s)	300, 313, 520, 1345, 1964, 1977, 2761
AlwNI	CAGNNNCTG	2 cut(s)	1186, 1583
Aor13HI	TCCGGA	2 cut(s)	1474, 2122
Aor51HI	AGCGCT	2 cut(s)	2289, 2515
AoxI	GGCC	6 cut(s)	319, 581, 1075, 1449, 1758, 2340
ApeKI	GCWGC	9 cut(s)	149, 152, 209, 1240, 1319, 1764, 2221, 2533, 3020
ApoI	RAATTY	8 cut(s)	341, 455, 1190, 1618, 2438, 2485, 2641, 2942
ArsI	GACNNNNNNTTYG	4 cut(s)	32, 64, 1861, 1893
AsiGI	ACCGGT	1 cut(s)	2261
Asp700I	GAANNNNTTC	2 cut(s)	504, 1797
AspLEI	GCGC	7 cut(s)	162, 542, 1729, 2290, 2516, 2573, 2832
AspS9I	GGNCC	4 cut(s)	925, 2120, 2232, 2750
AsuC2I	CCSGG	2 cut(s)	1369, 1983
AsuII	TTCGAA	1 cut(s)	1867
AvaII	GGWCC	4 cut(s)	925, 2120, 2232, 2750
AxyI	CCTNAGG	1 cut(s)	1233
BalI	TGGCCA	3 cut(s)	583, 1760, 2342
BamHI	GGATCC	2 cut(s)	305, 1969
BanI	GGYRCC	2 cut(s)	1739, 2570
BanII	GRGCYC	2 cut(s)	402, 819
BauI	CACGAG	1 cut(s)	2294
BbsI	GAAGAC	3 cut(s)	436, 2845, 2872
Bbv12I	GWGCWC	1 cut(s)	595
BbvCI	CCTCAGC	1 cut(s)	1140
BbvI	GCAGC	9 cut(s)	136, 139, 196, 1227, 1331, 1751, 2233, 2545, 3007
BceAI	ACGGC	4 cut(s)	122, 147, 1073, 1995
BcgI	CGANNNNNNTGC	2 cut(s)	134, 168
BciT130I	CCWGG	3 cut(s)	768, 2936, 2969
BciVI	GTATCC	1 cut(s)	632
BclI	TGATCA	2 cut(s)	1776, 2553
BcnI	CCSGG	2 cut(s)	1369, 1983
BcoDI	GTCTC	6 cut(s)	555, 789, 1322, 1627, 2040, 2629
BfaI	CTAG	4 cut(s)	1455, 1610, 1895, 2312
BfmI	CTRYAG	2 cut(s)	1031, 1575
BfoI	RGCGCY	3 cut(s)	2291, 2517, 2574
BfuAI	ACCTGC	2 cut(s)	266, 1024
BfuI	GTATCC	1 cut(s)	632
BglII	AGATCT	1 cut(s)	375
Bme1390I	CCNGG	5 cut(s)	768, 1369, 1983, 2936, 2969
Bme18I	GGWCC	4 cut(s)	925, 2120, 2232, 2750
BmgT120I	GGNCC	4 cut(s)	925, 2120, 2232, 2750
BmiI	GGNNCC	6 cut(s)	77, 307, 1741, 1971, 2572, 2711
BmrFI	CCNGG	5 cut(s)	768, 1369, 1983, 2936, 2969
BmrI	ACTGGG	2 cut(s)	311, 970
BmsI	GCATC	9 cut(s)	93, 372, 702, 790, 1162, 1173, 1823, 2269, 3000
BmuI	ACTGGG	2 cut(s)	311, 970
BoxI	GACNNNNGTC	1 cut(s)	923
BpiI	GAAGAC	3 cut(s)	436, 2845, 2872
BplI	GAGNNNNNCTC	2 cut(s)	2503, 2535
BpmI	CTGGAG	3 cut(s)	1174, 1896, 2064
Bpu10I	CCTNAGC	1 cut(s)	1140
Bpu14I	TTCGAA	1 cut(s)	1867
BpuMI	CCSGG	2 cut(s)	1369, 1983
BsaHI	GRCGYC	1 cut(s)	2571
BsaI	GGTCTC	1 cut(s)	789
BsaJI	CCNNGG	6 cut(s)	258, 529, 2316, 2893, 2935, 2967
BsaWI	WCCGGW	4 cut(s)	1474, 2122, 2261, 2752
BsaXI	ACNNNNNCTCC	6 cut(s)	786, 816, 1518, 1548, 2578, 2608
Bsc4I	CCNNNNNNNGG	8 cut(s)	812, 1033, 1715, 1934, 2458, 2627, 2706, 2968
Bse118I	RCCGGY	3 cut(s)	1520, 2261, 2623
Bse1I	ACTGG	9 cut(s)	306, 584, 868, 976, 1140, 1157, 1906, 2047, 2871
Bse21I	CCTNAGG	1 cut(s)	1233
Bse3DI	GCAATG	3 cut(s)	576, 1182, 2739
BseAI	TCCGGA	2 cut(s)	1474, 2122
BseBI	CCWGG	3 cut(s)	768, 2936, 2969
BseDI	CCNNGG	6 cut(s)	258, 529, 2316, 2893, 2935, 2967
BseGI	GGATG	5 cut(s)	972, 1692, 1814, 2377, 3015
BseLI	CCNNNNNNNGG	8 cut(s)	812, 1033, 1715, 1934, 2458, 2627, 2706, 2968
BseMI	GCAATG	3 cut(s)	576, 1182, 2739
BseMII	CTCAG	7 cut(s)	173, 992, 1131, 1224, 1712, 1758, 2325
BseNI	ACTGG	9 cut(s)	306, 584, 868, 976, 1140, 1157, 1906, 2047, 2871
BseRI	GAGGAG	2 cut(s)	2482, 2508
BseXI	GCAGC	9 cut(s)	136, 139, 196, 1227, 1331, 1751, 2233, 2545, 3007
BseYI	CCCAGC	1 cut(s)	2284
BsgI	GTGCAG	2 cut(s)	2240, 2552
Bsh1236I	CGCG	2 cut(s)	2830, 2832
BshFI	GGCC	6 cut(s)	321, 583, 1077, 1451, 1760, 2342
BshNI	GGYRCC	2 cut(s)	1739, 2570
BshTI	ACCGGT	1 cut(s)	2261
BsiHKAI	GWGCWC	1 cut(s)	595
BsiSI	CCGG	9 cut(s)	1368, 1475, 1521, 1983, 2123, 2262, 2624, 2753, 3024
BslFI	GGGAC	5 cut(s)	931, 1214, 1384, 1965, 2424
BslI	CCNNNNNNNGG	8 cut(s)	812, 1033, 1715, 1934, 2458, 2627, 2706, 2968
BsmAI	GTCTC	6 cut(s)	555, 789, 1322, 1627, 2040, 2629
BsmFI	GGGAC	5 cut(s)	931, 1214, 1384, 1965, 2424
BsmI	GAATGC	1 cut(s)	542
BsnI	GGCC	6 cut(s)	321, 583, 1077, 1451, 1760, 2342
Bso31I	GGTCTC	1 cut(s)	789
Bsp119I	TTCGAA	1 cut(s)	1867
Bsp1286I	GDGCHC	3 cut(s)	402, 595, 819
Bsp13I	TCCGGA	2 cut(s)	1474, 2122
Bsp19I	CCATGG	1 cut(s)	2316
BspACI	CCGC	9 cut(s)	109, 351, 661, 1011, 1244, 1387, 1524, 2230, 2621
BspANI	GGCC	6 cut(s)	321, 583, 1077, 1451, 1760, 2342
BspCNI	CTCAG	7 cut(s)	174, 993, 1132, 1225, 1713, 1757, 2326
BspEI	TCCGGA	2 cut(s)	1474, 2122
BspFNI	CGCG	2 cut(s)	2830, 2832
BspLI	GGNNCC	6 cut(s)	77, 307, 1741, 1971, 2572, 2711
BspMAI	CTGCAG	1 cut(s)	1035
BspMI	ACCTGC	2 cut(s)	266, 1024
BspPI	GGATC	7 cut(s)	300, 313, 520, 1345, 1964, 1977, 2761
BspQI	GCTCTTC	2 cut(s)	824, 1869
BspT104I	TTCGAA	1 cut(s)	1867
BspT107I	GGYRCC	2 cut(s)	1739, 2570
BspTNI	GGTCTC	1 cut(s)	789
BsrDI	GCAATG	3 cut(s)	576, 1182, 2739
BsrFI	RCCGGY	3 cut(s)	1520, 2261, 2623
BsrI	ACTGG	9 cut(s)	306, 584, 868, 976, 1140, 1157, 1906, 2047, 2871
BssAI	RCCGGY	3 cut(s)	1520, 2261, 2623
BssECI	CCNNGG	6 cut(s)	258, 529, 2316, 2893, 2935, 2967
BssNAI	GTATAC	1 cut(s)	1887
BssNI	GRCGYC	1 cut(s)	2571
BssSI	CACGAG	1 cut(s)	2294
BssT1I	CCWWGG	2 cut(s)	258, 2316
Bst1107I	GTATAC	1 cut(s)	1887
Bst2BI	CACGAG	1 cut(s)	2294
Bst2UI	CCWGG	3 cut(s)	768, 2936, 2969
Bst4CI	ACNGT	7 cut(s)	961, 1396, 2029, 2398, 2461, 2905, 2989
Bst6I	CTCTTC	2 cut(s)	824, 1869
BstACI	GRCGYC	1 cut(s)	2571
BstAPI	GCANNNNNTGC	1 cut(s)	2227
BstBI	TTCGAA	1 cut(s)	1867
BstC8I	GCNNGC	9 cut(s)	807, 1248, 1324, 1762, 1855, 2020, 2500, 2828, 2950
BstDSI	CCRYGG	1 cut(s)	2316
BstF5I	GGATG	5 cut(s)	972, 1692, 1814, 2377, 3015
BstFNI	CGCG	2 cut(s)	2830, 2832
BstH2I	RGCGCY	3 cut(s)	2291, 2517, 2574
BstHHI	GCGC	7 cut(s)	162, 542, 1729, 2290, 2516, 2573, 2832
BstMAI	GTCTC	6 cut(s)	555, 789, 1322, 1627, 2040, 2629
BstMWI	GCNNNNNNNGC	5 cut(s)	1039, 1859, 2227, 2452, 2954
BstNI	CCWGG	3 cut(s)	768, 2936, 2969
BstPAI	GACNNNNGTC	1 cut(s)	923
BstSCI	CCNGG	5 cut(s)	766, 1367, 1981, 2934, 2967
BstSFI	CTRYAG	2 cut(s)	1031, 1575
BstUI	CGCG	2 cut(s)	2830, 2832
BstV1I	GCAGC	9 cut(s)	136, 139, 196, 1227, 1331, 1751, 2233, 2545, 3007
BstV2I	GAAGAC	3 cut(s)	436, 2845, 2872
BstX2I	RGATCY	3 cut(s)	305, 375, 1969
BstYI	RGATCY	3 cut(s)	305, 375, 1969
BstZ17I	GTATAC	1 cut(s)	1887
Bsu36I	CCTNAGG	1 cut(s)	1233
BsuI	GTATCC	1 cut(s)	632
BsuRI	GGCC	6 cut(s)	321, 583, 1077, 1451, 1760, 2342
BtgI	CCRYGG	1 cut(s)	2316
BtgZI	GCGATG	2 cut(s)	349, 725
BtsCI	GGATG	5 cut(s)	972, 1692, 1814, 2377, 3015
BtsI	GCAGTG	2 cut(s)	120, 1363
BtsIMutI	CAGTG	7 cut(s)	120, 983, 1150, 1363, 2403, 2777, 2985
BveI	ACCTGC	2 cut(s)	266, 1024
Cac8I	GCNNGC	9 cut(s)	807, 1248, 1324, 1762, 1855, 2020, 2500, 2828, 2950
CaiI	CAGNNNCTG	2 cut(s)	1186, 1583
CfoI	GCGC	7 cut(s)	162, 542, 1729, 2290, 2516, 2573, 2832
Cfr10I	RCCGGY	3 cut(s)	1520, 2261, 2623
Cfr13I	GGNCC	4 cut(s)	925, 2120, 2232, 2750
CpoI	CGGWCCG	1 cut(s)	2750
CseI	GACGC	2 cut(s)	281, 2606
Csp6I	GTAC	2 cut(s)	165, 254
CspAI	ACCGGT	1 cut(s)	2261
CspI	CGGWCCG	1 cut(s)	2750
CviQI	GTAC	2 cut(s)	165, 254
DinI	GGCGCC	1 cut(s)	2572
DraIII	CACNNNGTG	1 cut(s)	1613
EaeI	YGGCCR	3 cut(s)	581, 1758, 2340
Eam1104I	CTCTTC	2 cut(s)	824, 1869
EarI	CTCTTC	2 cut(s)	824, 1869
EciI	GGCGGA	4 cut(s)	366, 1026, 1539, 2610
Eco130I	CCWWGG	2 cut(s)	258, 2316
Eco147I	AGGCCT	2 cut(s)	1077, 1451
Eco24I	GRGCYC	2 cut(s)	402, 819
Eco31I	GGTCTC	1 cut(s)	789
Eco47I	GGWCC	4 cut(s)	925, 2120, 2232, 2750
Eco47III	AGCGCT	2 cut(s)	2289, 2515
Eco57I	CTGAAG	4 cut(s)	72, 491, 2159, 2699
Eco81I	CCTNAGG	1 cut(s)	1233
EcoRI	GAATTC	1 cut(s)	1190
EcoRII	CCWGG	3 cut(s)	766, 2934, 2967
EcoT14I	CCWWGG	2 cut(s)	258, 2316
EcoT22I	ATGCAT	1 cut(s)	1412
EcoT38I	GRGCYC	2 cut(s)	402, 819
EgeI	GGCGCC	1 cut(s)	2572
EheI	GGCGCC	1 cut(s)	2572
ErhI	CCWWGG	2 cut(s)	258, 2316
FalI	AAGNNNNNCTT	2 cut(s)	2139, 2171
FaqI	GGGAC	5 cut(s)	931, 1214, 1384, 1965, 2424
FbaI	TGATCA	2 cut(s)	1776, 2553
FblI	GTMKAC	1 cut(s)	1886
FokI	GGATG	5 cut(s)	979, 1699, 1801, 2384, 3022
FriOI	GRGCYC	2 cut(s)	402, 819
FspAI	RTGCGCAY	1 cut(s)	541
FspBI	CTAG	4 cut(s)	1455, 1610, 1895, 2312
FspI	TGCGCA	1 cut(s)	541
GlaI	GCGC	7 cut(s)	161, 541, 1728, 2289, 2515, 2572, 2831
GsaI	CCCAGC	1 cut(s)	2288
GsuI	CTGGAG	3 cut(s)	1174, 1896, 2064
HaeII	RGCGCY	3 cut(s)	2291, 2517, 2574
HaeIII	GGCC	6 cut(s)	321, 583, 1077, 1451, 1760, 2342
HapII	CCGG	9 cut(s)	1368, 1475, 1521, 1983, 2123, 2262, 2624, 2753, 3024
HgaI	GACGC	2 cut(s)	281, 2606
HhaI	GCGC	7 cut(s)	162, 542, 1729, 2290, 2516, 2573, 2832
Hin1I	GRCGYC	1 cut(s)	2571
Hin6I	GCGC	7 cut(s)	160, 540, 1727, 2288, 2514, 2571, 2830
HinP1I	GCGC	7 cut(s)	160, 540, 1727, 2288, 2514, 2571, 2830
HincII	GTYRAC	2 cut(s)	1540, 2548
HindII	GTYRAC	2 cut(s)	1540, 2548
HindIII	AAGCTT	3 cut(s)	856, 1638, 1646
HpaI	GTTAAC	2 cut(s)	1540, 2548
HpaII	CCGG	9 cut(s)	1368, 1475, 1521, 1983, 2123, 2262, 2624, 2753, 3024
Hpy166II	GTNNAC	7 cut(s)	986, 1133, 1540, 1887, 2422, 2548, 2697
Hpy8I	GTNNAC	7 cut(s)	986, 1133, 1540, 1887, 2422, 2548, 2697
Hpy99I	CGWCG	2 cut(s)	254, 2888
HpyAV	CCTTC	9 cut(s)	34, 311, 515, 569, 1001, 1481, 1706, 2111, 2723
HpyCH4III	ACNGT	7 cut(s)	961, 1396, 2029, 2398, 2461, 2905, 2989
HpyCH4IV	ACGT	3 cut(s)	59, 249, 2413
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1039, 1859, 2227, 2452, 2954
HpySE526I	ACGT	3 cut(s)	59, 249, 2413
Hsp92I	GRCGYC	1 cut(s)	2571
HspAI	GCGC	7 cut(s)	160, 540, 1727, 2288, 2514, 2571, 2830
KasI	GGCGCC	1 cut(s)	2570
Kpn2I	TCCGGA	2 cut(s)	1474, 2122
Ksp22I	TGATCA	2 cut(s)	1776, 2553
KspAI	GTTAAC	2 cut(s)	1540, 2548
LguI	GCTCTTC	2 cut(s)	824, 1869
LmnI	GCTCC	5 cut(s)	590, 1155, 1702, 2511, 2521
Lsp1109I	GCAGC	9 cut(s)	136, 139, 196, 1227, 1331, 1751, 2233, 2545, 3007
LweI	GCATC	9 cut(s)	93, 372, 702, 790, 1162, 1173, 1823, 2269, 3000
MaeI	CTAG	4 cut(s)	1455, 1610, 1895, 2312
MaeII	ACGT	3 cut(s)	59, 249, 2413
MfeI	CAATTG	1 cut(s)	1212
MflI	RGATCY	3 cut(s)	305, 375, 1969
MhlI	GDGCHC	3 cut(s)	402, 595, 819
MlsI	TGGCCA	3 cut(s)	583, 1760, 2342
MluNI	TGGCCA	3 cut(s)	583, 1760, 2342
Mly113I	GGCGCC	1 cut(s)	2571
MlyI	GAGTC	4 cut(s)	511, 991, 2003, 2750
MmeI	TCCRAC	3 cut(s)	568, 1572, 2608
Mox20I	TGGCCA	3 cut(s)	583, 1760, 2342
Mph1103I	ATGCAT	1 cut(s)	1412
MroI	TCCGGA	2 cut(s)	1474, 2122
MroXI	GAANNNNTTC	2 cut(s)	504, 1797
MscI	TGGCCA	3 cut(s)	583, 1760, 2342
MseI	TTAA	8 cut(s)	860, 1512, 1539, 1653, 1965, 2463, 2547, 3029
MslI	CAYNNNNRTG	5 cut(s)	1415, 2528, 2555, 2795, 2906
Msp20I	TGGCCA	3 cut(s)	583, 1760, 2342
MspA1I	CMGCKG	5 cut(s)	1246, 1764, 2018, 2455, 2528
MspI	CCGG	9 cut(s)	1368, 1475, 1521, 1983, 2123, 2262, 2624, 2753, 3024
MspR9I	CCNGG	5 cut(s)	768, 1369, 1983, 2936, 2969
MunI	CAATTG	1 cut(s)	1212
Mva1269I	GAATGC	1 cut(s)	542
MvaI	CCWGG	3 cut(s)	768, 2936, 2969
MvnI	CGCG	2 cut(s)	2830, 2832
MwoI	GCNNNNNNNGC	5 cut(s)	1039, 1859, 2227, 2452, 2954
NarI	GGCGCC	1 cut(s)	2571
NciI	CCSGG	2 cut(s)	1369, 1983
NcoI	CCATGG	1 cut(s)	2316
NlaIV	GGNNCC	6 cut(s)	77, 307, 1741, 1971, 2572, 2711
NmuCI	GTSAC	7 cut(s)	640, 1093, 1252, 1358, 1605, 2257, 2983
NsbI	TGCGCA	1 cut(s)	541
NsiI	ATGCAT	1 cut(s)	1412
NspV	TTCGAA	1 cut(s)	1867
OliI	CACNNNNGTG	3 cut(s)	2528, 2795, 2906
PaqCI	CACCTGC	1 cut(s)	1024
PceI	AGGCCT	2 cut(s)	1077, 1451
PciSI	GCTCTTC	2 cut(s)	824, 1869
PctI	GAATGC	1 cut(s)	542
PdmI	GAANNNNTTC	2 cut(s)	504, 1797
PfeI	GAWTC	9 cut(s)	97, 410, 447, 609, 851, 1478, 1918, 2140, 2164
PflFI	GACNNNGTC	1 cut(s)	248
PflMI	CCANNNNNTGG	2 cut(s)	812, 2706
PinAI	ACCGGT	1 cut(s)	2261
PleI	GAGTC	4 cut(s)	511, 991, 2002, 2750
PluTI	GGCGCC	1 cut(s)	2574
PpsI	GAGTC	4 cut(s)	511, 991, 2002, 2750
PshAI	GACNNNNGTC	1 cut(s)	923
PsiI	TTATAA	1 cut(s)	2363
Psp1406I	AACGTT	1 cut(s)	59
Psp6I	CCWGG	3 cut(s)	766, 2934, 2967
PspFI	CCCAGC	1 cut(s)	2284
PspGI	CCWGG	3 cut(s)	766, 2934, 2967
PspN4I	GGNNCC	6 cut(s)	77, 307, 1741, 1971, 2572, 2711
PspPI	GGNCC	4 cut(s)	925, 2120, 2232, 2750
PstI	CTGCAG	1 cut(s)	1035
PstNI	CAGNNNCTG	2 cut(s)	1186, 1583
PsuI	RGATCY	3 cut(s)	305, 375, 1969
PsyI	GACNNNGTC	1 cut(s)	248
PvuII	CAGCTG	4 cut(s)	1764, 2018, 2455, 2528
RsaI	GTAC	2 cut(s)	166, 255
RsaNI	GTAC	2 cut(s)	165, 254
RseI	CAYNNNNRTG	5 cut(s)	1415, 2528, 2555, 2795, 2906
Rsr2I	CGGWCCG	1 cut(s)	2750
RsrII	CGGWCCG	1 cut(s)	2750
SapI	GCTCTTC	2 cut(s)	824, 1869
SaqAI	TTAA	8 cut(s)	860, 1512, 1539, 1653, 1965, 2463, 2547, 3029
Sau96I	GGNCC	4 cut(s)	925, 2120, 2232, 2750
SbfI	CCTGCAGG	1 cut(s)	1035
SchI	GAGTC	4 cut(s)	511, 991, 2003, 2750
ScrFI	CCNGG	5 cut(s)	768, 1369, 1983, 2936, 2969
SdaI	CCTGCAGG	1 cut(s)	1035
SduI	GDGCHC	3 cut(s)	402, 595, 819
SfaNI	GCATC	9 cut(s)	93, 372, 702, 790, 1162, 1173, 1823, 2269, 3000
SfcI	CTRYAG	2 cut(s)	1031, 1575
SfoI	GGCGCC	1 cut(s)	2572
SfuI	TTCGAA	1 cut(s)	1867
SgrAI	CRCCGGYG	2 cut(s)	1520, 2261
SinI	GGWCC	4 cut(s)	925, 2120, 2232, 2750
SmiMI	CAYNNNNRTG	5 cut(s)	1415, 2528, 2555, 2795, 2906
Sse8387I	CCTGCAGG	1 cut(s)	1035
SseBI	AGGCCT	2 cut(s)	1077, 1451
SsiI	CCGC	9 cut(s)	109, 351, 661, 1011, 1244, 1387, 1524, 2230, 2621
SspDI	GGCGCC	1 cut(s)	2570
SspI	AATATT	1 cut(s)	1351
SspMI	CTAG	4 cut(s)	1455, 1610, 1895, 2312
StuI	AGGCCT	2 cut(s)	1077, 1451
StyD4I	CCNGG	5 cut(s)	766, 1367, 1981, 2934, 2967
StyI	CCWWGG	2 cut(s)	258, 2316
TaaI	ACNGT	7 cut(s)	961, 1396, 2029, 2398, 2461, 2905, 2989
TaiI	ACGT	3 cut(s)	62, 252, 2416
TaqI	TCGA	6 cut(s)	144, 427, 1659, 1867, 2188, 2928
TauI	GCSGC	1 cut(s)	1246
TfiI	GAWTC	9 cut(s)	97, 410, 447, 609, 851, 1478, 1918, 2140, 2164
Tru1I	TTAA	8 cut(s)	860, 1512, 1539, 1653, 1965, 2463, 2547, 3029
Tru9I	TTAA	8 cut(s)	860, 1512, 1539, 1653, 1965, 2463, 2547, 3029
TscAI	CASTG	7 cut(s)	127, 983, 1157, 1363, 2403, 2784, 2992
TseFI	GTSAC	7 cut(s)	640, 1093, 1252, 1358, 1605, 2257, 2983
TseI	GCWGC	9 cut(s)	149, 152, 209, 1240, 1319, 1764, 2221, 2533, 3020
Tsp45I	GTSAC	7 cut(s)	640, 1093, 1252, 1358, 1605, 2257, 2983
TspDTI	ATGAA	9 cut(s)	312, 335, 1035, 1292, 1304, 1433, 2259, 2627, 2983
TspGWI	ACGGA	2 cut(s)	1699, 2763
TspRI	CASTG	7 cut(s)	127, 983, 1157, 1363, 2403, 2784, 2992
Tth111I	GACNNNGTC	1 cut(s)	248
Van91I	CCANNNNNTGG	2 cut(s)	812, 2706
VpaK11BI	GGWCC	4 cut(s)	925, 2120, 2232, 2750
XapI	RAATTY	8 cut(s)	341, 455, 1190, 1618, 2438, 2485, 2641, 2942
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2395
XmiI	GTMKAC	1 cut(s)	1886
XmnI	GAANNNNTTC	2 cut(s)	504, 1797
XspI	CTAG	4 cut(s)	1455, 1610, 1895, 2312
Zsp2I	ATGCAT	1 cut(s)	1412

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.