Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0011654

Physical Location & Seq

Genomic Coordinates Forward (+)

73332284 .. 73337251

Gene Structure 4968 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00135800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3090 bp

ATGGAGAGTTATTTGCAAGAGAACTTTGGGGAAGTGAAGGCTAAGGGCTCGTCTGAAGAAACGTTGCAGAAATGGAGGAAGCTCTGTGGAGTTGTCAAGAACCCGAAAAGAAGGTTTCGGTTCACTGCTAATCTTTCCAAGCGTATAGAGGCTGCGGCTATGCGCCGTACCAATCAGGCATGCCTTAACCTCCCCTCCCTAATATTGTGCATTGAGAAATTGAGGATTGCAGTTTTGGTTTCTAAAGCTGCATTTCAATTTATTCAAGGTGTACAGCCAAGTGACTATGTTCTACCTCAGGAAGTTAAAGCGGCAGGTTTTGGTATCTGTGCTGATGAATTGGGATCCATTGTGGAAGGCCATGATGTAAAGAAGCTAAAATTTCATGGAGGGGTAACTGGCCTTTCAGAAAAGCTCTCCACATCTATAAATGATGGGCTCAATACCGAAACTGATTTGCACACTCGAAGACAACAGATTTATGGCATTAATAAGTTTACAGAGAGCGAACAGCGTGGTTTCTTTGTATTTGTCTGGGAAGCCCTTCAGGACATGACTCTTATGATCCTCGGAGTATGTGCTTTTGTGTCATTGATCGTTGGACTAGCAATGGAAGGATGGCCAGCAGGAGCTCATGATGGGCTTGGAATTGTTGCGAGTATTATGCTGGTAGTGTTTGTCACAGCAACAAGTGATTATCGGCAATCACTACAGTTCAAGGACTTGGACAAGGAGAAGAAGAAGATTGCAATGCAAGTAACAAGAAATGGATATAGGCAGAAAATGTCGATTTATGAATTACTGCCTGGTGATATTGTACACCTTTCTATTGGAGACCAAGTACCTGCAGATGGTCTATTTGTTTCTGGTTTTTCTGTCTTAATTGATGAATCCAGTTTAACTGGTGAGAGTGAGCCTATTATGGTAACTCTTGAAAATCCCTTTCTTCTTTCTGGAACTAAGGTTCAAGATGGATCATGCAAGATGATGGTTACCACAGTAGGGATGAGAACTCAATGGGGCAAATTGATGGCTACACTAAGTGAAGGTGGAGATGATGAAACCCCATTGCAGGTCAAGTTGAATGGAGTGGCAACCATTATTGGTAAGATAGGACTTTTCTTTGCTGTTGTGACTTTTGCTGTTATGGTGCAAGGACTGTTTAGTCGAAAACTGCAAGAAGGGACACACTGGAGCTGGAATGGAGATGATGCAATGCAATTGTTAGAGTTCTTTGCTGTTGCAGTCACGATTGTTGTTGTTGCTGTCCCTGAGGGGCTTCCACTGGCTGTGACATTGAGTCTTGCATTTGCCATGAAGAAAATGATGAATGATAAGGCGCTTGTTCGCCATCTGGCAGCTTGCGAAACTATGGGATCAGCCACGAACATTTGCAGTGACAAAACTGGGACACTGACAACCAACAAAATGACTGTTGTGAAGTCATGCATTTGCATGAATATCAAAGATGTGAGCAACCTCAGTGATGCTTCAAGCTTGTTCTCTGATCTTCCGGAATCTGTTAAGAAGGTTTTGCTGCAATCAATATTTAACAACACTGGTGGAGAAGTTGTGGTTAGCAAAGGTGGCAAGAATGAGATGTTGGGAACACCTACCGACACTGCTTTGTTGGCGTTTGGCTTGGCACTTGGTGGAGATTTTCAAGCAGAAAGAAAAGCTTCTAAACTTGTGAAAGTTGAGCCTTTCAACTCAACGAAGAAAAGAATGGGGGTCGTGTTGGAGCTTCCTGAAGGAGGAGGTTTCAGAGCACATACTAAAGGTGCTTCAGAAATCATTTTGGCCAACTGTGACAAGGTGATCAATTCAAAGGGTGAAACTGTTCCTCTTGATGAAGCATCCATTAAGCATCTCCAAGCTACTATCAATGATTTTGCTTGTGAGGCTCTTCGAACTATCTGCCTTGGCTTTATGGAATTAGAAAAAGGATTCTCTGCTGAGGATCCCATTCCAACTTCTGGATATACTTGCATAGGAATTGTTGGCATTAAAGATCCTGTTCGCCCCGGTGTCAAGGAGTCTGTTGCAGTTTGCCGTTCAGCTGGTATCACAGTAAGAATGGTTACAGGAGACAATATTAACACTGCAAAGGCCATAGCAAGGGAATGTGGGATTCTCACTGAGGATGGATTAGCCATTGAAGGTCCGGAGTTCAGGGAGAAGAATCAAGAAGAATTGCTTTCACTGATACCTAAAATTCAGGTTATGGCGCGATCATCTCCTCTAGACAAGCATACCCTGGTAAAGCACTTGAGGACCACATTTGATGAAGTCGTTGCTGTAACTGGTGATGGAACAAATGATGCCCCAGCACTTCATGAGGCAGATATTGGACTAGCCATGGGCATTGCTGGAACTGAGGTGGCTAAGGAGAGTGCTGATGTTCTAATTCTGGATGATAATTTCTCCACAATTGTGACAGTGGCAAAATGGGGACGTTCGGTTTACATCAATATCCAAAAATTTGTGCAGTTCCAGCTGACTGTTAATATTGTTGCATTGATTGTTAACTTCTCCTCGGCCTGTTTGACAGGGAGTGCTCCCCTCACTGCTGTTCAGCTATTGTGGGTGAACATGATTATGGACACACTCGGAGCACTTGCACTTGCAACTGAGCCTCCAAATGACGACTTAATGAAGCGTCCGCCAGTTGGAAAGAGGCAAAATTTCATCAGTAATGTAATGTGGAGGAATATTTTAGGGCAGTCTTTCTATCAGTTTACAGTGTTATGGCTTCTTCAGGCTAAAGGAGAATCAATATTTCGTCTTAATGGATCAGATTCTAACTTGGTCCTGAACACACTCATTTTCAATACATTCGTCTTCTGTCAGGTGTTTAACGAAATAAGCTCGCGTGAGATGGAAGAAATAGATGTTTTCAAGGGCATATTGGATAATTACGTTTTCGTTGCAGTCCTCAGTGCCACAGTTGTGTTCCAAATCATTATTATCGAGTTTCTGGGGAAGTTTGCTAGCACAGCACCTCTCACCTTTGTTCAATGGTTTGTTACAGTATTCATTGGCTTTTTAGGGATGCCAATTGCTGCTGGTTTGAAGAAGATCCCTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007275 GO:0008150 GO:0008324 GO:0009555 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0031984 GO:0032501 GO:0032502 GO:0034220 GO:0042175 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048229 GO:0048856 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0098827 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1029

Amino Acids

112.01

Weight (kDa)

5.92

Isoelectric Point (pI)

36.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	5 - 49	6.3e-21	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	129 - 197	6.6e-10	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	250 - 346	4.3e-21	P-type ATPase actuator domain
Cation_ATPase	PF13246	527 - 675	8.1e-32	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	639 - 782	1.9e-11	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	853 - 1026	1.1e-42	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0011654)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22950 AT4G37640
fragaria_vesca	FvH4_1g17620
malus_domestica	MD02G1185000.v1.1 MD15G1296400.v1.1
prunus_persica	Prupe.6G221800_v2.0.a1 Prupe.6G221800_v2.0.a1
pyrus_communis	pycom02g14990 pycom15g25970
rosa_chinensis	RchiOBHm_Chr2g0107911
rosa_laevigata	RLG00000017627
rosa_multiflora	Rmu_sc0001484.1_g000032
rosa_roxburghii	Rroxscaffold_2G00135800
rosa_rugosa	Rorug02G0148700 Rorug02G0148800 Rorug02G0148800
rosa_samantha	Rh2BG210900 Rh2CG202700
rosa_wichuraiana	Rw2G015710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1164
Acc36I	ACCTGC	3 cut(s)	305, 853, 1063
AccB7I	CCANNNNNTGG	1 cut(s)	1976
AccII	CGCG	2 cut(s)	2230, 2872
AccIII	TCCGGA	2 cut(s)	1513, 2164
AciI	CCGC	3 cut(s)	155, 311, 2663
AclI	AACGTT	1 cut(s)	62
AcoI	YGGCCR	2 cut(s)	620, 1800
AcsI	RAATTY	4 cut(s)	380, 2214, 2480, 2683
AcuI	CTGAAG	5 cut(s)	75, 530, 1770, 1770, 2741
AdeI	CACNNNGTG	2 cut(s)	1043, 1652
AfaI	GTAC	4 cut(s)	169, 273, 819, 843
AfiI	CCNNNNNNNGG	7 cut(s)	851, 1002, 1072, 1754, 1976, 2118, 2669
AhdI	GACNNNNNGTC	1 cut(s)	1299
AjnI	CCWGG	2 cut(s)	805, 2256
AjuI	GAANNNNNNNTTGG	2 cut(s)	1586, 1618
AleI	CACNNNNGTG	2 cut(s)	2432, 2948
AloI	GAACNNNNNNTCC	2 cut(s)	948, 980
Alw21I	GWGCWC	4 cut(s)	634, 1771, 2560, 2617
Alw26I	GTCTC	2 cut(s)	828, 2082
Aor13HI	TCCGGA	2 cut(s)	1513, 2164
AoxI	GGCC	6 cut(s)	358, 400, 620, 1800, 2109, 2538
ApeKI	GCWGC	5 cut(s)	152, 248, 1358, 1537, 3062
ApoI	RAATTY	4 cut(s)	380, 2214, 2480, 2683
AseI	ATTAAT	1 cut(s)	489
Asp700I	GAANNNNTTC	3 cut(s)	543, 1678, 1839
AspLEI	GCGC	3 cut(s)	165, 1342, 2230
AspS9I	GGNCC	3 cut(s)	2162, 2274, 2809
AsuC2I	CCSGG	1 cut(s)	2025
AsuHPI	GGTGA	7 cut(s)	821, 917, 1828, 1844, 2318, 2599, 2998
AsuII	TTCGAA	1 cut(s)	1909
AsuNHI	GCTAGC	1 cut(s)	2990
AvaII	GGWCC	3 cut(s)	2162, 2274, 2809
AxyI	CCTNAGG	2 cut(s)	297, 1272
BalI	TGGCCA	2 cut(s)	622, 1802
BamHI	GGATCC	2 cut(s)	344, 1960
BanII	GRGCYC	3 cut(s)	50, 441, 634
BbsI	GAAGAC	2 cut(s)	475, 2833
Bbv12I	GWGCWC	4 cut(s)	634, 1771, 2560, 2617
BbvCI	CCTCAGC	1 cut(s)	1956
BbvI	GCAGC	5 cut(s)	139, 235, 1370, 1524, 3049
BceAI	ACGGC	2 cut(s)	150, 2037
BcgI	CGANNNNNNTGC	4 cut(s)	646, 680, 1899, 1933
BciT130I	CCWGG	2 cut(s)	807, 2258
BclI	TGATCA	1 cut(s)	1818
BcnI	CCSGG	1 cut(s)	2025
BcoDI	GTCTC	2 cut(s)	828, 2082
BfaI	CTAG	4 cut(s)	605, 2243, 2354, 2991
BfmI	CTRYAG	2 cut(s)	710, 846
BfoI	RGCGCY	1 cut(s)	1343
BfuAI	ACCTGC	3 cut(s)	305, 853, 1063
BisI	GCNGC	7 cut(s)	153, 156, 249, 312, 1359, 1538, 3063
BlsI	GCNGC	7 cut(s)	154, 157, 250, 313, 1360, 1539, 3064
Bme1390I	CCNGG	3 cut(s)	807, 2025, 2258
Bme18I	GGWCC	3 cut(s)	2162, 2274, 2809
BmeRI	GACNNNNNGTC	1 cut(s)	1299
BmgT120I	GGNCC	3 cut(s)	2162, 2274, 2809
BmiI	GGNNCC	2 cut(s)	346, 1962
BmrFI	CCNGG	3 cut(s)	807, 2025, 2258
BmrI	ACTGGG	1 cut(s)	1416
BmsI	GCATC	6 cut(s)	1201, 1477, 1865, 1876, 2311, 3042
BmtI	GCTAGC	1 cut(s)	2994
BmuI	ACTGGG	1 cut(s)	1416
BpiI	GAAGAC	2 cut(s)	475, 2833
BpmI	CTGGAG	1 cut(s)	1213
Bpu10I	CCTNAGC	3 cut(s)	42, 1956, 2385
Bpu14I	TTCGAA	1 cut(s)	1909
BpuEI	CTTGAG	1 cut(s)	2290
BpuMI	CCSGG	1 cut(s)	2025
BsaI	GGTCTC	1 cut(s)	828
BsaJI	CCNNGG	6 cut(s)	568, 1921, 2023, 2256, 2358, 2535
BsaWI	WCCGGW	2 cut(s)	1513, 2164
BsaXI	ACNNNNNCTCC	8 cut(s)	179, 209, 825, 855, 1557, 1587, 2620, 2650
Bsc4I	CCNNNNNNNGG	7 cut(s)	851, 1002, 1072, 1754, 1976, 2118, 2669
Bse1I	ACTGG	9 cut(s)	403, 894, 907, 1196, 1290, 1411, 1564, 2308, 2666
Bse21I	CCTNAGG	2 cut(s)	297, 1272
Bse3DI	GCAATG	5 cut(s)	615, 756, 1067, 1221, 2364
BseAI	TCCGGA	2 cut(s)	1513, 2164
BseBI	CCWGG	2 cut(s)	807, 2258
BseDI	CCNNGG	6 cut(s)	568, 1921, 2023, 2256, 2358, 2535
BseGI	GGATG	6 cut(s)	623, 1011, 1856, 2149, 2419, 3057
BseLI	CCNNNNNNNGG	7 cut(s)	851, 1002, 1072, 1754, 1976, 2118, 2669
BseMI	GCAATG	5 cut(s)	615, 756, 1067, 1221, 2364
BseMII	CTCAG	8 cut(s)	311, 1263, 1495, 1947, 2130, 2367, 2622, 2950
BseNI	ACTGG	9 cut(s)	403, 894, 907, 1196, 1290, 1411, 1564, 2308, 2666
BseRI	GAGGAG	3 cut(s)	1770, 2229, 2524
BseXI	GCAGC	5 cut(s)	139, 235, 1370, 1524, 3049
BseYI	CCCAGC	1 cut(s)	2326
BsgI	GTGCAG	1 cut(s)	2507
Bsh1236I	CGCG	2 cut(s)	2230, 2872
BshFI	GGCC	6 cut(s)	360, 402, 622, 1802, 2111, 2540
BsiHKAI	GWGCWC	4 cut(s)	634, 1771, 2560, 2617
BsiSI	CCGG	3 cut(s)	1514, 2025, 2165
BslFI	GGGAC	4 cut(s)	1198, 1253, 1423, 2466
BslI	CCNNNNNNNGG	7 cut(s)	851, 1002, 1072, 1754, 1976, 2118, 2669
BsmAI	GTCTC	2 cut(s)	828, 2082
BsmFI	GGGAC	4 cut(s)	1198, 1253, 1423, 2466
BsnI	GGCC	6 cut(s)	360, 402, 622, 1802, 2111, 2540
Bso31I	GGTCTC	1 cut(s)	828
Bsp119I	TTCGAA	1 cut(s)	1909
Bsp1286I	GDGCHC	6 cut(s)	50, 441, 634, 1771, 2560, 2617
Bsp13I	TCCGGA	2 cut(s)	1513, 2164
Bsp1407I	TGTACA	2 cut(s)	271, 817
Bsp19I	CCATGG	1 cut(s)	2358
BspACI	CCGC	3 cut(s)	155, 311, 2663
BspANI	GGCC	6 cut(s)	360, 402, 622, 1802, 2111, 2540
BspCNI	CTCAG	8 cut(s)	310, 1264, 1494, 1948, 2131, 2368, 2623, 2949
BspEI	TCCGGA	2 cut(s)	1513, 2164
BspFNI	CGCG	2 cut(s)	2230, 2872
BspHI	TCATGA	2 cut(s)	634, 2335
BspLI	GGNNCC	2 cut(s)	346, 1962
BspMAI	CTGCAG	1 cut(s)	850
BspMI	ACCTGC	3 cut(s)	305, 853, 1063
BspOI	GCTAGC	1 cut(s)	2994
BspQI	GCTCTTC	1 cut(s)	1911
BspT104I	TTCGAA	1 cut(s)	1909
BspTNI	GGTCTC	1 cut(s)	828
BsrDI	GCAATG	5 cut(s)	615, 756, 1067, 1221, 2364
BsrGI	TGTACA	2 cut(s)	271, 817
BsrI	ACTGG	9 cut(s)	403, 894, 907, 1196, 1290, 1411, 1564, 2308, 2666
BssECI	CCNNGG	6 cut(s)	568, 1921, 2023, 2256, 2358, 2535
BssT1I	CCWWGG	2 cut(s)	1921, 2358
Bst2UI	CCWGG	2 cut(s)	807, 2258
Bst6I	CTCTTC	1 cut(s)	1911
BstAUI	TGTACA	2 cut(s)	271, 817
BstBI	TTCGAA	1 cut(s)	1909
BstC8I	GCNNGC	5 cut(s)	181, 624, 1363, 2870, 2992
BstDSI	CCRYGG	1 cut(s)	2358
BstEII	GGTNACC	1 cut(s)	991
BstENI	CCTNNNNNAGG	1 cut(s)	1752
BstF5I	GGATG	6 cut(s)	623, 1011, 1856, 2149, 2419, 3057
BstFNI	CGCG	2 cut(s)	2230, 2872
BstH2I	RGCGCY	1 cut(s)	1343
BstHHI	GCGC	3 cut(s)	165, 1342, 2230
BstMAI	GTCTC	2 cut(s)	828, 2082
BstMWI	GCNNNNNNNGC	5 cut(s)	1587, 1631, 1901, 2494, 2996
BstNI	CCWGG	2 cut(s)	807, 2258
BstNSI	RCATGY	1 cut(s)	183
BstPI	GGTNACC	1 cut(s)	991
BstSCI	CCNGG	3 cut(s)	805, 2023, 2256
BstSFI	CTRYAG	2 cut(s)	710, 846
BstUI	CGCG	2 cut(s)	2230, 2872
BstV1I	GCAGC	5 cut(s)	139, 235, 1370, 1524, 3049
BstV2I	GAAGAC	2 cut(s)	475, 2833
BstX2I	RGATCY	4 cut(s)	344, 1960, 2011, 3078
BstYI	RGATCY	4 cut(s)	344, 1960, 2011, 3078
Bsu36I	CCTNAGG	2 cut(s)	297, 1272
BsuRI	GGCC	6 cut(s)	360, 402, 622, 1802, 2111, 2540
BtgI	CCRYGG	1 cut(s)	2358
BtsCI	GGATG	6 cut(s)	623, 1011, 1856, 2149, 2419, 3057
BtsI	GCAGTG	5 cut(s)	123, 1402, 1620, 2100, 2565
BveI	ACCTGC	3 cut(s)	305, 853, 1063
Cac8I	GCNNGC	5 cut(s)	181, 624, 1363, 2870, 2992
CciI	TCATGA	2 cut(s)	634, 2335
CfoI	GCGC	3 cut(s)	165, 1342, 2230
Cfr13I	GGNCC	3 cut(s)	2162, 2274, 2809
CseI	GACGC	1 cut(s)	2648
Csp6I	GTAC	4 cut(s)	168, 272, 818, 842
CspCI	CAANNNNNGTGG	2 cut(s)	1543, 1578
CviQI	GTAC	4 cut(s)	168, 272, 818, 842
DraIII	CACNNNGTG	2 cut(s)	1043, 1652
DrdI	GACNNNNNNGTC	1 cut(s)	1164
DriI	GACNNNNNGTC	1 cut(s)	1299
DseDI	GACNNNNNNGTC	1 cut(s)	1164
EaeI	YGGCCR	2 cut(s)	620, 1800
Eam1104I	CTCTTC	1 cut(s)	1911
Eam1105I	GACNNNNNGTC	1 cut(s)	1299
EarI	CTCTTC	1 cut(s)	1911
EciI	GGCGGA	1 cut(s)	2652
Ecl136II	GAGCTC	1 cut(s)	632
Eco130I	CCWWGG	2 cut(s)	1921, 2358
Eco24I	GRGCYC	3 cut(s)	50, 441, 634
Eco31I	GGTCTC	1 cut(s)	828
Eco47I	GGWCC	3 cut(s)	2162, 2274, 2809
Eco53kI	GAGCTC	1 cut(s)	632
Eco57I	CTGAAG	5 cut(s)	75, 530, 1770, 1770, 2741
Eco81I	CCTNAGG	2 cut(s)	297, 1272
Eco91I	GGTNACC	1 cut(s)	991
EcoICRI	GAGCTC	1 cut(s)	632
EcoNI	CCTNNNNNAGG	1 cut(s)	1752
EcoO65I	GGTNACC	1 cut(s)	991
EcoRII	CCWGG	2 cut(s)	805, 2256
EcoT14I	CCWWGG	2 cut(s)	1921, 2358
EcoT22I	ATGCAT	1 cut(s)	1451
EcoT38I	GRGCYC	3 cut(s)	50, 441, 634
ErhI	CCWWGG	2 cut(s)	1921, 2358
FalI	AAGNNNNNCTT	4 cut(s)	1663, 1695, 2181, 2213
FaqI	GGGAC	4 cut(s)	1198, 1253, 1423, 2466
FbaI	TGATCA	1 cut(s)	1818
Fnu4HI	GCNGC	7 cut(s)	153, 156, 249, 312, 1359, 1538, 3063
FokI	GGATG	6 cut(s)	630, 1018, 1843, 2156, 2426, 3064
FriOI	GRGCYC	3 cut(s)	50, 441, 634
Fsp4HI	GCNGC	7 cut(s)	153, 156, 249, 312, 1359, 1538, 3063
FspBI	CTAG	4 cut(s)	605, 2243, 2354, 2991
GlaI	GCGC	3 cut(s)	164, 1341, 2229
GluI	GCNGC	7 cut(s)	153, 156, 249, 312, 1359, 1538, 3063
GsaI	CCCAGC	1 cut(s)	2330
GsuI	CTGGAG	1 cut(s)	1213
HaeII	RGCGCY	1 cut(s)	1343
HaeIII	GGCC	6 cut(s)	360, 402, 622, 1802, 2111, 2540
HapII	CCGG	3 cut(s)	1514, 2025, 2165
HgaI	GACGC	1 cut(s)	2648
HhaI	GCGC	3 cut(s)	165, 1342, 2230
Hin6I	GCGC	3 cut(s)	163, 1340, 2228
HinP1I	GCGC	3 cut(s)	163, 1340, 2228
HincII	GTYRAC	1 cut(s)	2527
HindII	GTYRAC	1 cut(s)	2527
HindIII	AAGCTT	2 cut(s)	1495, 1677
HpaI	GTTAAC	1 cut(s)	2527
HpaII	CCGG	3 cut(s)	1514, 2025, 2165
HphI	GGTGA	7 cut(s)	821, 917, 1828, 1844, 2318, 2599, 2998
Hpy166II	GTNNAC	8 cut(s)	123, 272, 498, 820, 2464, 2527, 2590, 2739
Hpy188I	TCNGA	8 cut(s)	55, 409, 572, 1507, 1766, 1789, 2612, 2797
Hpy8I	GTNNAC	8 cut(s)	123, 272, 498, 820, 2464, 2527, 2590, 2739
HpyCH4IV	ACGT	3 cut(s)	62, 2455, 2919
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1587, 1631, 1901, 2494, 2996
HpySE526I	ACGT	3 cut(s)	62, 2455, 2919
HspAI	GCGC	3 cut(s)	163, 1340, 2228
Kpn2I	TCCGGA	2 cut(s)	1513, 2164
Ksp22I	TGATCA	1 cut(s)	1818
KspAI	GTTAAC	1 cut(s)	2527
LguI	GCTCTTC	1 cut(s)	1911
LmnI	GCTCC	5 cut(s)	629, 1194, 1741, 2563, 2612
Lsp1109I	GCAGC	5 cut(s)	139, 235, 1370, 1524, 3049
LweI	GCATC	6 cut(s)	1201, 1477, 1865, 1876, 2311, 3042
MaeI	CTAG	4 cut(s)	605, 2243, 2354, 2991
MaeII	ACGT	3 cut(s)	62, 2455, 2919
MfeI	CAATTG	3 cut(s)	1220, 2430, 3057
MflI	RGATCY	4 cut(s)	344, 1960, 2011, 3078
MhlI	GDGCHC	6 cut(s)	50, 441, 634, 1771, 2560, 2617
MlsI	TGGCCA	2 cut(s)	622, 1802
MluNI	TGGCCA	2 cut(s)	622, 1802
MlyI	GAGTC	3 cut(s)	550, 1309, 2045
MmeI	TCCRAC	4 cut(s)	580, 1719, 1994, 2650
Mox20I	TGGCCA	2 cut(s)	622, 1802
Mph1103I	ATGCAT	1 cut(s)	1451
MroI	TCCGGA	2 cut(s)	1513, 2164
MroXI	GAANNNNTTC	3 cut(s)	543, 1678, 1839
MscI	TGGCCA	2 cut(s)	622, 1802
MslI	CAYNNNNRTG	4 cut(s)	1454, 2432, 2597, 2948
Msp20I	TGGCCA	2 cut(s)	622, 1802
MspA1I	CMGCKG	2 cut(s)	2060, 2497
MspI	CCGG	3 cut(s)	1514, 2025, 2165
MspR9I	CCNGG	3 cut(s)	807, 2025, 2258
MunI	CAATTG	3 cut(s)	1220, 2430, 3057
MvaI	CCWGG	2 cut(s)	807, 2258
MvnI	CGCG	2 cut(s)	2230, 2872
MwoI	GCNNNNNNNGC	5 cut(s)	1587, 1631, 1901, 2494, 2996
NciI	CCSGG	1 cut(s)	2025
NcoI	CCATGG	1 cut(s)	2358
NheI	GCTAGC	1 cut(s)	2990
NlaIV	GGNNCC	2 cut(s)	346, 1962
NmeAIII	GCCGAG	1 cut(s)	2516
NmuCI	GTSAC	8 cut(s)	281, 679, 1132, 1246, 1291, 1397, 1808, 2434
NsiI	ATGCAT	1 cut(s)	1451
NspI	RCATGY	1 cut(s)	183
NspV	TTCGAA	1 cut(s)	1909
OliI	CACNNNNGTG	2 cut(s)	2432, 2948
PaeI	GCATGC	1 cut(s)	183
PagI	TCATGA	2 cut(s)	634, 2335
PciSI	GCTCTTC	1 cut(s)	1911
PdmI	GAANNNNTTC	3 cut(s)	543, 1678, 1839
PfeI	GAWTC	7 cut(s)	890, 1517, 1947, 2131, 2182, 2771, 2798
PflMI	CCANNNNNTGG	1 cut(s)	1976
PkrI	GCNGC	7 cut(s)	154, 157, 250, 313, 1360, 1539, 3064
PleI	GAGTC	3 cut(s)	550, 1308, 2044
PpsI	GAGTC	3 cut(s)	550, 1308, 2044
PshBI	ATTAAT	1 cut(s)	489
Psp124BI	GAGCTC	1 cut(s)	634
Psp1406I	AACGTT	1 cut(s)	62
Psp6I	CCWGG	2 cut(s)	805, 2256
PspEI	GGTNACC	1 cut(s)	991
PspFI	CCCAGC	1 cut(s)	2326
PspGI	CCWGG	2 cut(s)	805, 2256
PspN4I	GGNNCC	2 cut(s)	346, 1962
PspPI	GGNCC	3 cut(s)	2162, 2274, 2809
PstI	CTGCAG	1 cut(s)	850
PsuI	RGATCY	4 cut(s)	344, 1960, 2011, 3078
PvuII	CAGCTG	2 cut(s)	2060, 2497
RsaI	GTAC	4 cut(s)	169, 273, 819, 843
RsaNI	GTAC	4 cut(s)	168, 272, 818, 842
RseI	CAYNNNNRTG	4 cut(s)	1454, 2432, 2597, 2948
SacI	GAGCTC	1 cut(s)	634
SapI	GCTCTTC	1 cut(s)	1911
SatI	GCNGC	7 cut(s)	153, 156, 249, 312, 1359, 1538, 3063
Sau96I	GGNCC	3 cut(s)	2162, 2274, 2809
SchI	GAGTC	3 cut(s)	550, 1309, 2045
ScrFI	CCNGG	3 cut(s)	807, 2025, 2258
SduI	GDGCHC	6 cut(s)	50, 441, 634, 1771, 2560, 2617
SfaNI	GCATC	6 cut(s)	1201, 1477, 1865, 1876, 2311, 3042
SfcI	CTRYAG	2 cut(s)	710, 846
SfuI	TTCGAA	1 cut(s)	1909
SinI	GGWCC	3 cut(s)	2162, 2274, 2809
SmiMI	CAYNNNNRTG	4 cut(s)	1454, 2432, 2597, 2948
SmlI	CTYRAG	1 cut(s)	2269
SmoI	CTYRAG	1 cut(s)	2269
SphI	GCATGC	1 cut(s)	183
SsiI	CCGC	3 cut(s)	155, 311, 2663
SspI	AATATT	6 cut(s)	204, 1548, 2095, 2509, 2713, 2778
SspMI	CTAG	4 cut(s)	605, 2243, 2354, 2991
SstI	GAGCTC	1 cut(s)	634
StyD4I	CCNGG	3 cut(s)	805, 2023, 2256
StyI	CCWWGG	2 cut(s)	1921, 2358
TaiI	ACGT	3 cut(s)	65, 2458, 2922
TaqI	TCGA	5 cut(s)	466, 788, 1168, 1909, 2970
TatI	WGTACW	2 cut(s)	271, 817
TauI	GCSGC	2 cut(s)	158, 314
TfiI	GAWTC	7 cut(s)	890, 1517, 1947, 2131, 2182, 2771, 2798
TseFI	GTSAC	8 cut(s)	281, 679, 1132, 1246, 1291, 1397, 1808, 2434
TseI	GCWGC	5 cut(s)	152, 248, 1358, 1537, 3062
Tsp45I	GTSAC	8 cut(s)	281, 679, 1132, 1246, 1291, 1397, 1808, 2434
Van91I	CCANNNNNTGG	1 cut(s)	1976
VpaK11BI	GGWCC	3 cut(s)	2162, 2274, 2809
VspI	ATTAAT	1 cut(s)	489
XagI	CCTNNNNNAGG	1 cut(s)	1752
XapI	RAATTY	4 cut(s)	380, 2214, 2480, 2683
XbaI	TCTAGA	1 cut(s)	2242
XceI	RCATGY	1 cut(s)	183
XmnI	GAANNNNTTC	3 cut(s)	543, 1678, 1839
XspI	CTAG	4 cut(s)	605, 2243, 2354, 2991
Zsp2I	ATGCAT	1 cut(s)	1451

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.