MD02G1189000.v1.1

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016538
Physical Location & Seq
Forward (+)
17268175 .. 17269458
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1189000.v1.1.491

Sequence Viewer

Length: 1284 bp
ATGGACCTGCAATTGGCGATAAACGGTGTCGATTTTTCCCGGAAAAGGACGAAGTGGCTCGTTGTAGCGGCCGCTTTAGGCTTCTCCGGTTATGGTGTGTATAGGTTATATCACTTGCCTTCAGTATCTCAGAAAAGAAAGAGAATTTTAAAGCTTCTAGGGGCATTAGTTTCCGTAGCTGAAGTAGTTTCTGACTCTGCTGAGTCAATTGGAGTTGTGTCTAAGGATTTGAAGGACTTTCTGAAGTCTGATTCTGATAAAATTCCAAATAGTTTAAAGCAATTATCGAAAATCGCTAGGTGTGATGAGTTTTCGGAATCTGTTGTTACGGTCACGCAGGCTGTAAGTTTGGGGGTATTGCGGGGTTATGGATTGAACACAACGAGTGATGGGGATGGTGGAGATTCAAGCTTTACAGACCAGGTTATGGATAAGCTTTTTACCAAGGCCGGGTCGGGTTTTGCTTCTGTTGTTGTTGGAAACTTTGCTAGGAATGTGGTATTAGCTTTCTATTCAGATGGGAAGTCTGGTGGAGGATCTAACTCAAACAATTCGTCGAGTATGGACGATTATGTGACGGAAATGGATGATGTTCCGAGATGGGTGGATGTGGTTTCGAGCGAAAAGTTCAGAGACTTGATTGGTGATTGTATCCAGTTATTTGTAAGCACAGCTGTTGCGGTTTATCTTGACAAGACCATGGGTATCAACACCTATGATGACATCTTTTCTGGGTTAACCAACTCCAAGCATGAAACAAAAGTGAGAGACATGCTGTCGTCTGTCTGCAATGGCGCGGTGGAAACTCTTATCAGAACATCTCACCAAGTATTAACAGATTCAACTTCAAATGCAAACCCTTCGAAATCAAACTCGAGTTCCTATTTGGCAATTGATGAAGGCTCAACTCCAACTAGAGACGAGGGCTTGAGGCAACAGGCATTATCGTTGCATTCAAAGGCAAGAAATGTGGAGAATGAGAGGAACAATAGCGGTTGGGTTGGTAAAGTGTCCACTACTTTGGCAGTGCCAAGCAACCGGCGGTTTGTTCTTGATGTCACCGGGAGGGTTACATCTGAAACAGTCAGATCATTCCTGGAGTTTTTGTTGGACAGACTAAACGATGGGATGAAAAAATGCGTTAATTCTGTTCGTGAGGAAACCGTGGACAGGGGTCTTGAGGTAGTGAGATATGCTGCTGCAAAATCTTCTGTTATTGCTACTATATCTCTGTCTTTGTGCTTACACATACTTGACAGTCCATGGGCATTGGTTCCATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

428

Amino Acids

46.3

Weight (kDa)

6.26

Isoelectric Point (pI)

28.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016538)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49790
fragaria_vesca FvH4_1g18080
malus_domestica MD02G1189000.v1.1
prunus_persica Prupe.6G217700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0108551
rosa_laevigata RLG00000017678
rosa_multiflora Rmu_sc0007574.1_g000007
rosa_roxburghii Rroxscaffold_2G00135030
rosa_rugosa Rorug02G0154400
rosa_samantha Rh2AG203300 Rh2BG216200 Rh2CG207200 Rh2DG210400
rosa_wichuraiana Rw2G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 15
AccB7I CCANNNNNTGG 1 cut(s) 427
AccII CGCG 1 cut(s) 797
AciI CCGC 7 cut(s) 68, 72, 361, 680, 797, 993, 1042
AclWI GGATC 1 cut(s) 544
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 2 cut(s) 144, 261
AcuI CTGAAG 3 cut(s) 105, 201, 263
AfiI CCNNNNNNNGG 5 cut(s) 13, 45, 427, 450, 1170
AgsI TTSAA 6 cut(s) 232, 376, 408, 843, 849, 957
AhdI GACNNNNNGTC 1 cut(s) 775
AjnI CCWGG 2 cut(s) 420, 1095
AluBI AGCT 6 cut(s) 154, 179, 411, 436, 506, 674
AluI AGCT 6 cut(s) 154, 179, 411, 436, 506, 674
Alw26I GTCTC 3 cut(s) 627, 762, 912
AlwI GGATC 1 cut(s) 544
Ama87I CYCGRG 1 cut(s) 874
AoxI GGCC 2 cut(s) 69, 447
ApeKI GCWGC 2 cut(s) 1196, 1199
ApoI RAATTY 2 cut(s) 144, 261
ArsI GACNNNNNNTTYG 2 cut(s) 539, 571
AspLEI GCGC 1 cut(s) 797
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 3 cut(s) 40, 451, 1063
AsuHPI GGTGA 3 cut(s) 656, 815, 1051
AsuII TTCGAA 1 cut(s) 863
AvaI CYCGRG 1 cut(s) 874
AvaII GGWCC 1 cut(s) 4
BbvI GCAGC 2 cut(s) 1183, 1186
BccI CCATC 5 cut(s) 383, 389, 512, 594, 1118
BcgI CGANNNNNNTGC 2 cut(s) 843, 877
BciT130I CCWGG 2 cut(s) 422, 1097
BciVI GTATCC 1 cut(s) 662
BcnI CCSGG 3 cut(s) 40, 451, 1063
BcoDI GTCTC 3 cut(s) 627, 762, 912
BfaI CTAG 4 cut(s) 158, 297, 489, 915
BfuAI ACCTGC 1 cut(s) 15
BfuI GTATCC 1 cut(s) 662
BisI GCNGC 4 cut(s) 69, 72, 1197, 1200
BlsI GCNGC 4 cut(s) 70, 73, 1198, 1201
Bme1390I CCNGG 5 cut(s) 40, 422, 451, 1063, 1097
Bme18I GGWCC 1 cut(s) 4
BmeRI GACNNNNNGTC 1 cut(s) 775
BmeT110I CYCGRG 1 cut(s) 874
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 1275
BmrFI CCNGG 5 cut(s) 40, 422, 451, 1063, 1097
BoxI GACNNNNGTC 1 cut(s) 1173
BpmI CTGGAG 1 cut(s) 1118
Bpu14I TTCGAA 1 cut(s) 863
BpuEI CTTGAG 2 cut(s) 949, 1199
BpuMI CCSGG 3 cut(s) 40, 451, 1063
BsaJI CCNNGG 4 cut(s) 444, 699, 1164, 1262
BsaWI WCCGGW 1 cut(s) 86
BsaXI ACNNNNNCTCC 2 cut(s) 1091, 1121
Bsc4I CCNNNNNNNGG 5 cut(s) 13, 45, 427, 450, 1170
Bse118I RCCGGY 1 cut(s) 1038
Bse1I ACTGG 1 cut(s) 655
Bse3DI GCAATG 1 cut(s) 796
BseBI CCWGG 2 cut(s) 422, 1097
BseDI CCNNGG 4 cut(s) 444, 699, 1164, 1262
BseGI GGATG 4 cut(s) 400, 592, 613, 1134
BseLI CCNNNNNNNGG 5 cut(s) 13, 45, 427, 450, 1170
BseMI GCAATG 1 cut(s) 796
BseMII CTCAG 2 cut(s) 143, 192
BseNI ACTGG 1 cut(s) 655
BseX3I CGGCCG 1 cut(s) 69
BseXI GCAGC 2 cut(s) 1183, 1186
Bsh1236I CGCG 1 cut(s) 797
Bsh1285I CGRYCG 1 cut(s) 72
BshFI GGCC 2 cut(s) 71, 449
BsiEI CGRYCG 1 cut(s) 72
BsiHKCI CYCGRG 1 cut(s) 874
BsiSI CCGG 5 cut(s) 40, 87, 450, 1039, 1062
BslI CCNNNNNNNGG 5 cut(s) 13, 45, 427, 450, 1170
BsmAI GTCTC 3 cut(s) 627, 762, 912
BsmBI CGTCTC 1 cut(s) 912
BsmI GAATGC 1 cut(s) 952
BsnI GGCC 2 cut(s) 71, 449
BsoBI CYCGRG 1 cut(s) 874
Bsp119I TTCGAA 1 cut(s) 863
Bsp143I GATC 2 cut(s) 536, 1088
Bsp19I CCATGG 2 cut(s) 699, 1262
BspACI CCGC 7 cut(s) 68, 72, 361, 680, 797, 993, 1042
BspANI GGCC 2 cut(s) 71, 449
BspCNI CTCAG 2 cut(s) 142, 193
BspFNI CGCG 1 cut(s) 797
BspLI GGNNCC 1 cut(s) 1275
BspMI ACCTGC 1 cut(s) 15
BspPI GGATC 1 cut(s) 544
BspT104I TTCGAA 1 cut(s) 863
BsrDI GCAATG 1 cut(s) 796
BsrFI RCCGGY 1 cut(s) 1038
BsrI ACTGG 1 cut(s) 655
BssAI RCCGGY 1 cut(s) 1038
BssECI CCNNGG 4 cut(s) 444, 699, 1164, 1262
BssMI GATC 2 cut(s) 536, 1088
BssT1I CCWWGG 3 cut(s) 444, 699, 1262
Bst2UI CCWGG 2 cut(s) 422, 1097
Bst4CI ACNGT 5 cut(s) 26, 331, 1084, 1165, 1259
BstBI TTCGAA 1 cut(s) 863
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 4 cut(s) 129, 201, 222, 1281
BstDSI CCRYGG 3 cut(s) 699, 1164, 1262
BstF5I GGATG 4 cut(s) 400, 592, 613, 1134
BstFNI CGCG 1 cut(s) 797
BstHHI GCGC 1 cut(s) 797
BstKTI GATC 2 cut(s) 539, 1091
BstMAI GTCTC 3 cut(s) 627, 762, 912
BstMBI GATC 2 cut(s) 536, 1088
BstMCI CGRYCG 1 cut(s) 72
BstNI CCWGG 2 cut(s) 422, 1097
BstNSI RCATGY 1 cut(s) 775
BstPAI GACNNNNGTC 1 cut(s) 1173
BstSCI CCNGG 5 cut(s) 38, 420, 449, 1061, 1095
BstUI CGCG 1 cut(s) 797
BstV1I GCAGC 2 cut(s) 1183, 1186
BstX2I RGATCY 1 cut(s) 536
BstXI CCANNNNNNTGG 1 cut(s) 1021
BstYI RGATCY 1 cut(s) 536
BstZI CGGCCG 1 cut(s) 69
BsuI GTATCC 1 cut(s) 662
BsuRI GGCC 2 cut(s) 71, 449
BtgI CCRYGG 3 cut(s) 699, 1164, 1262
BtsCI GGATG 4 cut(s) 400, 592, 613, 1134
BtsI GCAGTG 1 cut(s) 1032
BtsIMutI CAGTG 1 cut(s) 1032
BveI ACCTGC 1 cut(s) 15
Cac8I GCNNGC 1 cut(s) 339
CciNI GCGGCCGC 1 cut(s) 69
CfoI GCGC 1 cut(s) 797
Cfr10I RCCGGY 1 cut(s) 1038
Cfr13I GGNCC 1 cut(s) 4
CsiI ACCWGGT 1 cut(s) 420
CspCI CAANNNNNGTGG 2 cut(s) 951, 986
CviAII CATG 4 cut(s) 700, 752, 772, 1263
DdeI CTNAG 4 cut(s) 129, 201, 222, 1281
DpnI GATC 2 cut(s) 538, 1090
DpnII GATC 2 cut(s) 536, 1088
DraI TTTAAA 2 cut(s) 150, 276
DriI GACNNNNNGTC 1 cut(s) 775
EaeI YGGCCR 1 cut(s) 69
EagI CGGCCG 1 cut(s) 69
Eam1105I GACNNNNNGTC 1 cut(s) 775
EclXI CGGCCG 1 cut(s) 69
Eco130I CCWWGG 3 cut(s) 444, 699, 1262
Eco47I GGWCC 1 cut(s) 4
Eco52I CGGCCG 1 cut(s) 69
Eco57I CTGAAG 3 cut(s) 105, 201, 263
Eco88I CYCGRG 1 cut(s) 874
EcoRII CCWGG 2 cut(s) 420, 1095
EcoT14I CCWWGG 3 cut(s) 444, 699, 1262
ErhI CCWWGG 3 cut(s) 444, 699, 1262
Esp3I CGTCTC 1 cut(s) 912
FaeI CATG 4 cut(s) 703, 755, 775, 1266
FatI CATG 4 cut(s) 699, 751, 771, 1262
FauI CCCGC 1 cut(s) 354
Fnu4HI GCNGC 4 cut(s) 69, 72, 1197, 1200
FokI GGATG 4 cut(s) 407, 599, 620, 1141
Fsp4HI GCNGC 4 cut(s) 69, 72, 1197, 1200
FspBI CTAG 4 cut(s) 158, 297, 489, 915
GlaI GCGC 1 cut(s) 796
GluI GCNGC 4 cut(s) 69, 72, 1197, 1200
GsuI CTGGAG 1 cut(s) 1118
HaeIII GGCC 2 cut(s) 71, 449
HapII CCGG 5 cut(s) 40, 87, 450, 1039, 1062
HhaI GCGC 1 cut(s) 797
Hin1II CATG 4 cut(s) 703, 755, 775, 1266
Hin6I GCGC 1 cut(s) 795
HinP1I GCGC 1 cut(s) 795
HincII GTYRAC 1 cut(s) 738
HindII GTYRAC 1 cut(s) 738
HindIII AAGCTT 3 cut(s) 152, 409, 434
HinfI GANTC 6 cut(s) 194, 203, 251, 317, 404, 839
HpaI GTTAAC 1 cut(s) 738
HpaII CCGG 5 cut(s) 40, 87, 450, 1039, 1062
HphI GGTGA 3 cut(s) 656, 815, 1051
Hpy166II GTNNAC 3 cut(s) 738, 1014, 1168
Hpy188III TCNNGA 4 cut(s) 689, 1052, 1154, 1178
Hpy8I GTNNAC 3 cut(s) 738, 1014, 1168
Hpy99I CGWCG 1 cut(s) 559
HpyAV CCTTC 4 cut(s) 129, 226, 870, 893
HpyCH4III ACNGT 5 cut(s) 26, 331, 1084, 1165, 1259
HpyCH4V TGCA 5 cut(s) 10, 789, 854, 952, 1202
HpyF3I CTNAG 4 cut(s) 129, 201, 222, 1281
Hsp92II CATG 4 cut(s) 703, 755, 775, 1266
HspAI GCGC 1 cut(s) 795
KspAI GTTAAC 1 cut(s) 738
Kzo9I GATC 2 cut(s) 536, 1088
Lsp1109I GCAGC 2 cut(s) 1183, 1186
MabI ACCWGGT 1 cut(s) 420
MaeI CTAG 4 cut(s) 158, 297, 489, 915
MaeIII GTNAC 5 cut(s) 325, 331, 574, 1057, 1069
MalI GATC 2 cut(s) 538, 1090
MboI GATC 2 cut(s) 536, 1088
MboII GAAGA 1 cut(s) 1200
MfeI CAATTG 3 cut(s) 11, 207, 891
MflI RGATCY 1 cut(s) 536
MluCI AATT 8 cut(s) 11, 144, 207, 261, 281, 550, 891, 1144
MlyI GAGTC 2 cut(s) 188, 212
MmeI TCCRAC 3 cut(s) 457, 935, 1089
MnlI CCTC 7 cut(s) 527, 916, 924, 975, 1059, 1150, 1174
MseI TTAA 5 cut(s) 149, 275, 737, 833, 1143
MspA1I CMGCKG 1 cut(s) 674
MspI CCGG 5 cut(s) 40, 87, 450, 1039, 1062
MspR9I CCNGG 5 cut(s) 40, 422, 451, 1063, 1097
MunI CAATTG 3 cut(s) 11, 207, 891
Mva1269I GAATGC 1 cut(s) 952
MvaI CCWGG 2 cut(s) 422, 1097
MvnI CGCG 1 cut(s) 797
NciI CCSGG 3 cut(s) 40, 451, 1063
NcoI CCATGG 2 cut(s) 699, 1262
NdeII GATC 2 cut(s) 536, 1088
NlaIII CATG 4 cut(s) 703, 755, 775, 1266
NlaIV GGNNCC 1 cut(s) 1275
NmuCI GTSAC 3 cut(s) 331, 574, 1057
NotI GCGGCCGC 1 cut(s) 69
NspI RCATGY 1 cut(s) 775
NspV TTCGAA 1 cut(s) 863
PaeR7I CTCGAG 1 cut(s) 874
PctI GAATGC 1 cut(s) 952
PfeI GAWTC 4 cut(s) 251, 317, 404, 839
PflMI CCANNNNNTGG 1 cut(s) 427
PfoI TCCNGGA 2 cut(s) 38, 1095
PkrI GCNGC 4 cut(s) 70, 73, 1198, 1201
PleI GAGTC 2 cut(s) 188, 211
PpsI GAGTC 2 cut(s) 188, 211
PshAI GACNNNNGTC 1 cut(s) 1173
Psp6I CCWGG 2 cut(s) 420, 1095
PspGI CCWGG 2 cut(s) 420, 1095
PspN4I GGNNCC 1 cut(s) 1275
PspPI GGNCC 1 cut(s) 4
PspXI VCTCGAGB 1 cut(s) 874
PsuI RGATCY 1 cut(s) 536
PvuII CAGCTG 1 cut(s) 674
SaqAI TTAA 5 cut(s) 149, 275, 737, 833, 1143
SatI GCNGC 4 cut(s) 69, 72, 1197, 1200
Sau3AI GATC 2 cut(s) 536, 1088
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 2 cut(s) 188, 212
ScrFI CCNGG 5 cut(s) 40, 422, 451, 1063, 1097
SexAI ACCWGGT 1 cut(s) 420
Sfr274I CTCGAG 1 cut(s) 874
SfuI TTCGAA 1 cut(s) 863
SinI GGWCC 1 cut(s) 4
SlaI CTCGAG 1 cut(s) 874
SmlI CTYRAG 3 cut(s) 874, 928, 1178
SmoI CTYRAG 3 cut(s) 874, 928, 1178
Sse9I AATT 8 cut(s) 11, 144, 207, 261, 281, 550, 891, 1144
SsiI CCGC 7 cut(s) 68, 72, 361, 680, 797, 993, 1042
SspMI CTAG 4 cut(s) 158, 297, 489, 915
StyD4I CCNGG 5 cut(s) 38, 420, 449, 1061, 1095
StyI CCWWGG 3 cut(s) 444, 699, 1262
TaaI ACNGT 5 cut(s) 26, 331, 1084, 1165, 1259
TaqI TCGA 6 cut(s) 30, 287, 557, 617, 863, 875
TasI AATT 8 cut(s) 11, 144, 207, 261, 281, 550, 891, 1144
TauI GCSGC 2 cut(s) 71, 74
TfiI GAWTC 4 cut(s) 251, 317, 404, 839
Tru1I TTAA 5 cut(s) 149, 275, 737, 833, 1143
Tru9I TTAA 5 cut(s) 149, 275, 737, 833, 1143
TscAI CASTG 1 cut(s) 1032
TseFI GTSAC 3 cut(s) 331, 574, 1057
TseI GCWGC 2 cut(s) 1196, 1199
Tsp45I GTSAC 3 cut(s) 331, 574, 1057
TspDTI ATGAA 3 cut(s) 768, 912, 1145
TspGWI ACGGA 2 cut(s) 163, 593
TspRI CASTG 1 cut(s) 1032
Van91I CCANNNNNTGG 1 cut(s) 427
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 2 cut(s) 144, 261
XceI RCATGY 1 cut(s) 775
XhoI CTCGAG 1 cut(s) 874
XspI CTAG 4 cut(s) 158, 297, 489, 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.