Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0008730

Physical Location & Seq

Genomic Coordinates Reverse (-)

17840606 .. 17846806

Gene Structure 6201 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1191400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3228 bp

ATGGCGTCTCCACAGTCGGAGCTCTTTGCGAGGACGCCGAGTTCCGGTAGGGCTCTGTCCATAACGCCGGGGTCTAGGATTTTGCAGGGCCCTGGCGCCGTCGGTGATGAGGCGATCTGGAAGAGGTTTAAAGAAGTTGGATTTGACGAGGAATCGATTAAACGGAGGGACAAGGCGGCGCTCATTACATATATTGCGAAGCTTGAAGCAGAGATTTTCGACCACCAACACCACATGGGCCTTCTCATATTGGAAAGAAAGGAGTTGGCATCCAATTATGAGCAAATTAAAGCCTCTGCCAAGACCGCTGAACTTTTGCATAAGCGTGATAAAGCTGCAGATTTATCTGCTTTGGCTGATGCAAGTAAACGGGAGGAGTGCCTAAAAAAGACAGTAGGTGTCAAGGAAGAGTGTATAGCTAGTATTGAGAAGACAATGCATGAGCAGCGTGCAGAATCTGCTGAAACGAAGGTTGCAGCTGACAGTAAATTGGCTGAAGCACGGAAAGTGGTGGAGGATGCTGAGAAGAAATTCACTGATGCTGAGGGAAAACTGCATGCAGCAGAATCTTTGCAAGCGGAAGCTTGTCGCTTTCACCGTATTGCTGAAAGAAAGATGCGAGAAGTTGAAGCTCGTGAAGATGATCTTAGGAGGAACATTCTGTCATTCAAGACGGACTGTGAAACAAAAGAGAAAGAGATAAGCCTTGAGAGACAATCTTTAAATGAAAGGCAGAAATCCTTGCAGCAAGAACAAGATAGGTTACTTGATGCACAAGCTCTGCTAAACCAAAGGGAGGATTTCATATTTGGTCGATCTCAGGAACTGAATCAACTTGAAAAAGAGTTAGAAGACGTAAAAGCAAATATTGAGAAGGAGCGTAGAGCCCTAGATGATGAAAAATTGAATCTGGAGCTAACTGGGGCTTCCCTTATTAATAGAGAGGAGGCTTTAACTAGAAGAGAAGTTCTATTGAACAAAAAAGAGCAAGAGATACTTGCTTTGCAGGAAAAACTTGTGAGCAAGGAATCTGATGTGATTAAGAAAGCTATAGCAAGTCACGAAGCTGATTTGAGGAAAAAGAAGTCTGAATTTGAAACGGAGTTGGACGTGAAGCTGAAATCTTTTGAAGATAAAATTGAGGCCAAGAGACGGGCTTGGGAGTTGAGGGAGGTTGATCTCAATCAACGCGAAGATCTACTGCATGAAAGGGAGCATGACTTGGAAGTTCAGTTGAGGGCATTGATGGATAGGGAGAAAGAAGTGGCAGAGATGTCAAATCTTGTTGGTGAGAAAGAAAATAATTTGAGAGAGGCTGAGAAGGAGTTTGAACAGAATGGTGGTATGTTGCAAATAGAAAAGGAAGAAATTATCAAAATGAAGCTAGAACTTCAAAGGTCCTTGGATTCACTTGAAGAAAAAAGGAAACAACTTGATTGTGCTAGGGAGAGGTTTGAGCTTTTGAAATCTGAAACTAGTGAGCTTTCAGATCTTGAGATGAAGCTTAAAGAAGAAATAGATTTAGTCAGGGCTCAAAAGCTAGAGTTGATGGCGGAGGCAGATAAATTATCTATAGAGAAAGCGAAGTTTGAATCTGAGTGGGAATTAATTGATGAAAAGAGAGAAGAGCTGCGTAAGGAAGCGGAGCGTGTAGCAGAGGAGAGGTTGGCGTTCTCAAAATTTATTAAGGATGAGCATGATAACTTGAGACGGGAGAAGGATGAAATGCGAGATCGGCACAAACGGGATGTGGAATCACTTGTTAGTGAGCGGGAGGACTTCATGAGTAAGATGGTTCACGAGCGTACTGAGTGGTTCAGTAAGATGCAGAAGGAGCGTGCAGATTTCTTACTGGAAATTGAGATACGGAAGGGAGAGTTGGAGAACTGTATAGATAAAAAACATGAAGAGTTAGAATGTTCATTGAAGGAAAAGGAGATTGCCTTTGAGCAAGAAAAGAAAAATGAAGTTGAGATTATCAGCTTCCTCAAGGAAGAATCTGCAAAAGAGAGGGACCAGGTTGCTCTAGAAAGGAAAAGGCTTGAGTCTGAGAGAATAGAAATAAACGTGGATCGTAAGAGAAGGGATTGTGAATGGGCAGAGCTATGTAATTCGATTGAGGAACTGAGGGTTCAGAGGGAGAAACTAAAAGAACAGAGGGAATTATTGCACGCTGATAGAGAAGAGATTCTTGGTCTTATTCAACACCTGAAGGAACTGCAACATTTGAAGGCTGCTTTAGATAGTGCACCTGTGGCTGAGATGCAACAATCTGATTTGGTGCCTCACAGCCGGAAAACTTCTAGACGATCTTTGAAGCAAACTTCTTCTGTACGAGACGCTGATGTTAATTTGCATAATGAAGTAAACGCTGACAACATTAGCAACCAGTCCATGCTAAAGACAGGGCTGTCTCCTTCCAGTTCTGCTCGCTTCTCATGGTTAAGACGTTGGAGGGAACTGATGTTTAAAAATTCTCCTGAGAAGCACCAATTGGAATTTGAAGAAAGCCCTGTGATCTCTCTGGAAAAAACAAGCTTGACAGTAACTGAACAGGTAGAAACATCAAGTAAATATGATGGGCATCGGAACATGGGAAATGGCAATTCACCTGCATTTTCCAGCAAGAGACAGTGTGCTTTTGGTGAACCAAAGGTTATTGTTGAAGTGCCCTTTGTTGGTGACCTTTCTAATGGAACAAAGGATTCTGAATCTGAAATTAAGGAATTTGATGGTGAAAGTTGTGGCCCCATAGTCTCAGAACAAGTCTTCAAGGGAGGAAGGAAAAGAAGAGTGGACAAATCTTCGTCCGCTGGCTGTTTTGATCCACTCCTTGAGCCTAGGCAGAATGTTAAGAAAAGGAGGCAACAGCAGGATGCCGTTCCGAATTCCTCAGAACATGCCAATAGCCAGTGCGTTGCATCAGTGCCATTTCATCAAATTCATGAAGATGCTGAAGAGGGTACTGTTTCAATAGTAGATAAAGTCACAAAGGTTACTAAAGTGATTTTTGGGGAGACTTTTACTGGCACTCTTTCCAGTGAAGACAAATCAGAGGCACAGAATTCTGTTGTGGAACCAGACCATGTAAAGAATGGTGTCTTACCCGGTGACACTAAGGCTCAAGAAAAGATGCAAGAATCGAACTTGGGGGATGCTGGACAATTTATTGACCACTGTCAGACTCAAGAGGAAGATATAACCGAAAAGAATTTGCAGTCCGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005652 GO:0005694 GO:0005737 GO:0006996 GO:0006997 GO:0008150 GO:0009507 GO:0009536 GO:0009987 GO:0010369 GO:0016043 GO:0031974 GO:0031981 GO:0032535 GO:0034399 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0065007 GO:0065008 GO:0070013 GO:0071840 GO:0090066 GO:0097298 GO:0098687

KEGG Pathways

Metabolic & Signaling

ko00270 ko00280 ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00270 map00280 map00290 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1076

Amino Acids

124.08

Weight (kDa)

5.3

Isoelectric Point (pI)

57.93

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008730)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G65770 AT5G65770 AT5G65770 AT5G65780
fragaria_vesca	FvH4_1g18512 FvH4_1g18512
malus_domestica	MD02G1191400.v1.1 MD15G1302600.v1.1
prunus_persica	Prupe.6G214100_v2.0.a1 Prupe.6G214100_v2.0.a1
pyrus_communis	pycom15g26560
rosa_chinensis	RchiOBHm_Chr2g0109151
rosa_laevigata	RLG00000017717
rosa_multiflora	Rmu_sc0000186.1_g000019
rosa_roxburghii	Rroxscaffold_2G00134460
rosa_rugosa	Rorug02G0157500 Rorug02G0157500 Rorug02G0157500 Rorug02G0157600
rosa_samantha	Rh2AG209600 Rh2BG220000 Rh2CG212100 Rh2DG215700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2620
AasI	GACNNNNNNGTC	1 cut(s)	2410
Acc36I	ACCTGC	1 cut(s)	2620
AccB1I	GGYRCC	2 cut(s)	95, 2281
AccBSI	CCGCTC	1 cut(s)	1771
AccII	CGCG	1 cut(s)	1191
AciI	CCGC	7 cut(s)	176, 306, 578, 1553, 1643, 1771, 2811
AclWI	GGATC	2 cut(s)	2079, 2819
AcuI	CTGAAG	3 cut(s)	516, 2231, 2976
AcyI	GRCGYC	3 cut(s)	5, 35, 96
AfaI	GTAC	3 cut(s)	1807, 2334, 2965
AfiI	CCNNNNNNNGG	4 cut(s)	44, 796, 1152, 2678
AhlI	ACTAGT	1 cut(s)	1475
AjiI	CACGTC	1 cut(s)	1111
AjnI	CCWGG	2 cut(s)	91, 2016
AjuI	GAANNNNNNNTTGG	6 cut(s)	1862, 1894, 2175, 2207, 2477, 2509
AloI	GAACNNNNNNTCC	2 cut(s)	25, 57
Alw21I	GWGCWC	2 cut(s)	24, 2251
Alw26I	GTCTC	9 cut(s)	12, 706, 1144, 1702, 2331, 2418, 2623, 2761, 3011
Alw44I	GTGCAC	1 cut(s)	2247
AlwI	GGATC	2 cut(s)	2079, 2819
AlwNI	CAGNNNCTG	3 cut(s)	458, 826, 2549
AoxI	GGCC	4 cut(s)	88, 238, 1143, 2746
ApaI	GGGCCC	1 cut(s)	92
ApaLI	GTGCAC	1 cut(s)	2247
ApeKI	GCWGC	7 cut(s)	335, 445, 476, 560, 745, 1630, 2234
AseI	ATTAAT	2 cut(s)	936, 1607
Asp700I	GAANNNNTTC	2 cut(s)	530, 2187
AspA2I	CCTAGG	1 cut(s)	2840
AspLEI	GCGC	2 cut(s)	98, 181
AspS9I	GGNCC	6 cut(s)	88, 89, 238, 1398, 2014, 2747
AsuC2I	CCSGG	2 cut(s)	69, 3108
AsuHPI	GGTGA	8 cut(s)	116, 587, 1301, 2601, 2657, 2693, 2747, 3122
AvaII	GGWCC	2 cut(s)	1398, 2014
AvrII	CCTAGG	1 cut(s)	2840
BaeGI	GKGCMC	3 cut(s)	92, 2251, 2673
BanI	GGYRCC	2 cut(s)	95, 2281
BanII	GRGCYC	5 cut(s)	24, 55, 92, 889, 1534
BauI	CACGAG	2 cut(s)	633, 1799
BbsI	GAAGAC	4 cut(s)	437, 858, 2761, 3051
Bbv12I	GWGCWC	2 cut(s)	24, 2251
BbvCI	CCTCAGC	1 cut(s)	543
BbvI	GCAGC	7 cut(s)	322, 457, 488, 572, 757, 1617, 2221
BccI	CCATC	5 cut(s)	1240, 1543, 1786, 2573, 2726
BceAI	ACGGC	2 cut(s)	83, 2864
BciT130I	CCWGG	2 cut(s)	93, 2018
BcnI	CCSGG	2 cut(s)	69, 3108
BcoDI	GTCTC	9 cut(s)	12, 706, 1144, 1702, 2331, 2418, 2623, 2761, 3011
BcuI	ACTAGT	1 cut(s)	1475
BfmI	CTRYAG	3 cut(s)	336, 1050, 1572
BfoI	RGCGCY	2 cut(s)	99, 182
BfuAI	ACCTGC	1 cut(s)	2620
BglII	AGATCT	2 cut(s)	1195, 1489
BisI	GCNGC	8 cut(s)	177, 336, 446, 477, 561, 746, 1631, 2235
BlnI	CCTAGG	1 cut(s)	2840
BlsI	GCNGC	8 cut(s)	178, 337, 447, 478, 562, 747, 1632, 2236
Bme1390I	CCNGG	4 cut(s)	69, 93, 2018, 3108
Bme18I	GGWCC	2 cut(s)	1398, 2014
BmgBI	CACGTC	1 cut(s)	1111
BmgT120I	GGNCC	6 cut(s)	88, 89, 238, 1398, 2014, 2747
BmiI	GGNNCC	6 cut(s)	90, 97, 2015, 2283, 2749, 3078
BmrFI	CCNGG	4 cut(s)	69, 93, 2018, 3108
BmrI	ACTGGG	1 cut(s)	930
BmuI	ACTGGG	1 cut(s)	930
BoxI	GACNNNNGTC	1 cut(s)	3177
BpiI	GAAGAC	4 cut(s)	437, 858, 2761, 3051
BpmI	CTGGAG	1 cut(s)	932
Bpu10I	CCTNAGC	1 cut(s)	543
BpuEI	CTTGAG	8 cut(s)	728, 1514, 1726, 1973, 2063, 2855, 3108, 3171
BpuMI	CCSGG	2 cut(s)	69, 3108
Bsa29I	ATCGAT	1 cut(s)	155
BsaBI	GATNNNNATC	2 cut(s)	1182, 2583
BsaHI	GRCGYC	3 cut(s)	5, 35, 96
BsaJI	CCNNGG	4 cut(s)	68, 91, 1401, 2840
BsaWI	WCCGGW	1 cut(s)	44
Bsc4I	CCNNNNNNNGG	4 cut(s)	44, 796, 1152, 2678
Bse1I	ACTGG	7 cut(s)	925, 1857, 2389, 2421, 2911, 3031, 3039
Bse8I	GATNNNNATC	2 cut(s)	1182, 2583
BseBI	CCWGG	2 cut(s)	93, 2018
BseCI	ATCGAT	1 cut(s)	155
BseDI	CCNNGG	4 cut(s)	68, 91, 1401, 2840
BseGI	GGATG	7 cut(s)	269, 523, 1696, 1726, 1753, 2881, 3160
BseJI	GATNNNNATC	2 cut(s)	1182, 2583
BseLI	CCNNNNNNNGG	4 cut(s)	44, 796, 1152, 2678
BseNI	ACTGG	7 cut(s)	925, 1857, 2389, 2421, 2911, 3031, 3039
BseRI	GAGGAG	3 cut(s)	389, 959, 1673
BseSI	GKGCMC	3 cut(s)	92, 2251, 2673
BseXI	GCAGC	7 cut(s)	322, 457, 488, 572, 757, 1617, 2221
BsgI	GTGCAG	2 cut(s)	471, 1860
Bsh1236I	CGCG	1 cut(s)	1191
BshFI	GGCC	4 cut(s)	90, 240, 1145, 2748
BshNI	GGYRCC	2 cut(s)	95, 2281
BshVI	ATCGAT	1 cut(s)	155
BsiHKAI	GWGCWC	2 cut(s)	24, 2251
BsiSI	CCGG	4 cut(s)	45, 68, 2293, 3108
BslFI	GGGAC	2 cut(s)	182, 2027
BslI	CCNNNNNNNGG	4 cut(s)	44, 796, 1152, 2678
BsmAI	GTCTC	9 cut(s)	12, 706, 1144, 1702, 2331, 2418, 2623, 2761, 3011
BsmBI	CGTCTC	4 cut(s)	12, 1144, 1702, 2331
BsmFI	GGGAC	2 cut(s)	182, 2027
BsnI	GGCC	4 cut(s)	90, 240, 1145, 2748
Bsp120I	GGGCCC	1 cut(s)	88
Bsp1286I	GDGCHC	7 cut(s)	24, 55, 92, 889, 1534, 2251, 2673
BspACI	CCGC	7 cut(s)	176, 306, 578, 1553, 1643, 1771, 2811
BspANI	GGCC	4 cut(s)	90, 240, 1145, 2748
BspDI	ATCGAT	1 cut(s)	155
BspFNI	CGCG	1 cut(s)	1191
BspHI	TCATGA	2 cut(s)	1782, 2944
BspLI	GGNNCC	6 cut(s)	90, 97, 2015, 2283, 2749, 3078
BspMAI	CTGCAG	1 cut(s)	340
BspMI	ACCTGC	1 cut(s)	2620
BspPI	GGATC	2 cut(s)	2079, 2819
BspQI	GCTCTTC	1 cut(s)	1620
BspT107I	GGYRCC	2 cut(s)	95, 2281
BsrBI	CCGCTC	1 cut(s)	1771
BsrI	ACTGG	7 cut(s)	925, 1857, 2389, 2421, 2911, 3031, 3039
BssECI	CCNNGG	4 cut(s)	68, 91, 1401, 2840
BssNI	GRCGYC	3 cut(s)	5, 35, 96
BssSI	CACGAG	2 cut(s)	633, 1799
BssT1I	CCWWGG	2 cut(s)	1401, 2840
Bst2BI	CACGAG	2 cut(s)	633, 1799
Bst2UI	CCWGG	2 cut(s)	93, 2018
Bst6I	CTCTTC	8 cut(s)	116, 402, 955, 1620, 1902, 2178, 2785, 2952
BstACI	GRCGYC	3 cut(s)	5, 35, 96
BstAPI	GCANNNNNTGC	1 cut(s)	458
BstC8I	GCNNGC	7 cut(s)	450, 558, 576, 1839, 2172, 2431, 2815
BstEII	GGTNACC	1 cut(s)	2681
BstF5I	GGATG	7 cut(s)	269, 523, 1696, 1726, 1753, 2881, 3160
BstFNI	CGCG	1 cut(s)	1191
BstH2I	RGCGCY	2 cut(s)	99, 182
BstHHI	GCGC	2 cut(s)	98, 181
BstMAI	GTCTC	9 cut(s)	12, 706, 1144, 1702, 2331, 2418, 2623, 2761, 3011
BstMWI	GCNNNNNNNGC	6 cut(s)	305, 445, 458, 1735, 1834, 2255
BstNI	CCWGG	2 cut(s)	93, 2018
BstNSI	RCATGY	2 cut(s)	560, 2903
BstPAI	GACNNNNGTC	1 cut(s)	3177
BstPI	GGTNACC	1 cut(s)	2681
BstSCI	CCNGG	4 cut(s)	67, 91, 2016, 3106
BstSFI	CTRYAG	3 cut(s)	336, 1050, 1572
BstSLI	GKGCMC	3 cut(s)	92, 2251, 2673
BstUI	CGCG	1 cut(s)	1191
BstV1I	GCAGC	7 cut(s)	322, 457, 488, 572, 757, 1617, 2221
BstV2I	GAAGAC	4 cut(s)	437, 858, 2761, 3051
BstX2I	RGATCY	2 cut(s)	1195, 1489
BstYI	RGATCY	2 cut(s)	1195, 1489
Bsu15I	ATCGAT	1 cut(s)	155
BsuRI	GGCC	4 cut(s)	90, 240, 1145, 2748
BsuTUI	ATCGAT	1 cut(s)	155
BtrI	CACGTC	1 cut(s)	1111
BtsCI	GGATG	7 cut(s)	269, 523, 1696, 1726, 1753, 2881, 3160
BtsIMutI	CAGTG	6 cut(s)	534, 2639, 2918, 2931, 3046, 3175
BveI	ACCTGC	1 cut(s)	2620
Cac8I	GCNNGC	7 cut(s)	450, 558, 576, 1839, 2172, 2431, 2815
CaiI	CAGNNNCTG	3 cut(s)	458, 826, 2549
CciI	TCATGA	2 cut(s)	1782, 2944
CfoI	GCGC	2 cut(s)	98, 181
Cfr13I	GGNCC	6 cut(s)	88, 89, 238, 1398, 2014, 2747
ClaI	ATCGAT	1 cut(s)	155
CseI	GACGC	2 cut(s)	43, 2348
CsiI	ACCWGGT	1 cut(s)	2016
Csp6I	GTAC	3 cut(s)	1806, 2333, 2964
CviQI	GTAC	3 cut(s)	1806, 2333, 2964
DinI	GGCGCC	1 cut(s)	97
DraI	TTTAAA	3 cut(s)	130, 723, 2470
DrdI	GACNNNNNNGTC	1 cut(s)	2410
DseDI	GACNNNNNNGTC	1 cut(s)	2410
Eam1104I	CTCTTC	8 cut(s)	116, 402, 955, 1620, 1902, 2178, 2785, 2952
EarI	CTCTTC	8 cut(s)	116, 402, 955, 1620, 1902, 2178, 2785, 2952
EciI	GGCGGA	1 cut(s)	1568
Ecl136II	GAGCTC	1 cut(s)	22
Eco130I	CCWWGG	2 cut(s)	1401, 2840
Eco24I	GRGCYC	5 cut(s)	24, 55, 92, 889, 1534
Eco47I	GGWCC	2 cut(s)	1398, 2014
Eco53kI	GAGCTC	1 cut(s)	22
Eco57I	CTGAAG	3 cut(s)	516, 2231, 2976
Eco91I	GGTNACC	1 cut(s)	2681
EcoICRI	GAGCTC	1 cut(s)	22
EcoO109I	RGGNCCY	3 cut(s)	88, 89, 1398
EcoO65I	GGTNACC	1 cut(s)	2681
EcoRI	GAATTC	2 cut(s)	2887, 3064
EcoRII	CCWGG	2 cut(s)	91, 2016
EcoT14I	CCWWGG	2 cut(s)	1401, 2840
EcoT22I	ATGCAT	1 cut(s)	441
EcoT38I	GRGCYC	5 cut(s)	24, 55, 92, 889, 1534
EgeI	GGCGCC	1 cut(s)	97
EheI	GGCGCC	1 cut(s)	97
ErhI	CCWWGG	2 cut(s)	1401, 2840
Esp3I	CGTCTC	4 cut(s)	12, 1144, 1702, 2331
FalI	AAGNNNNNCTT	6 cut(s)	630, 662, 981, 1013, 2175, 2207
FaqI	GGGAC	2 cut(s)	182, 2027
FauI	CCCGC	1 cut(s)	1764
Fnu4HI	GCNGC	8 cut(s)	177, 336, 446, 477, 561, 746, 1631, 2235
FokI	GGATG	7 cut(s)	256, 530, 1703, 1733, 1760, 2888, 3167
FriOI	GRGCYC	5 cut(s)	24, 55, 92, 889, 1534
Fsp4HI	GCNGC	8 cut(s)	177, 336, 446, 477, 561, 746, 1631, 2235
GlaI	GCGC	2 cut(s)	97, 180
GluI	GCNGC	8 cut(s)	177, 336, 446, 477, 561, 746, 1631, 2235
GsuI	CTGGAG	1 cut(s)	932
HaeII	RGCGCY	2 cut(s)	99, 182
HaeIII	GGCC	4 cut(s)	90, 240, 1145, 2748
HapII	CCGG	4 cut(s)	45, 68, 2293, 3108
HgaI	GACGC	2 cut(s)	43, 2348
HhaI	GCGC	2 cut(s)	98, 181
Hin1I	GRCGYC	3 cut(s)	5, 35, 96
Hin6I	GCGC	2 cut(s)	96, 179
HinP1I	GCGC	2 cut(s)	96, 179
HindIII	AAGCTT	4 cut(s)	200, 582, 1502, 2536
HpaII	CCGG	4 cut(s)	45, 68, 2293, 3108
HphI	GGTGA	8 cut(s)	116, 587, 1301, 2601, 2657, 2693, 2747, 3122
Hpy166II	GTNNAC	6 cut(s)	368, 1798, 2249, 2368, 2648, 2797
Hpy8I	GTNNAC	6 cut(s)	368, 1798, 2249, 2368, 2648, 2797
Hpy99I	CGWCG	1 cut(s)	104
HpyCH4IV	ACGT	4 cut(s)	855, 1110, 2067, 2449
HpyF10VI	GCNNNNNNNGC	6 cut(s)	305, 445, 458, 1735, 1834, 2255
HpySE526I	ACGT	4 cut(s)	855, 1110, 2067, 2449
Hsp92I	GRCGYC	3 cut(s)	5, 35, 96
HspAI	GCGC	2 cut(s)	96, 179
KasI	GGCGCC	1 cut(s)	95
LguI	GCTCTTC	1 cut(s)	1620
LmnI	GCTCC	6 cut(s)	19, 877, 913, 1213, 1645, 1834
Lsp1109I	GCAGC	7 cut(s)	322, 457, 488, 572, 757, 1617, 2221
MabI	ACCWGGT	1 cut(s)	2016
MaeII	ACGT	4 cut(s)	855, 1110, 2067, 2449
MaeIII	GTNAC	7 cut(s)	762, 1058, 2545, 2681, 2986, 2995, 3110
MbiI	CCGCTC	1 cut(s)	1771
MfeI	CAATTG	1 cut(s)	2492
MflI	RGATCY	2 cut(s)	1195, 1489
MhlI	GDGCHC	7 cut(s)	24, 55, 92, 889, 1534, 2251, 2673
Mly113I	GGCGCC	1 cut(s)	96
MlyI	GAGTC	2 cut(s)	2054, 3178
MmeI	TCCRAC	4 cut(s)	118, 1086, 1860, 2432
Mph1103I	ATGCAT	1 cut(s)	441
MroXI	GAANNNNTTC	2 cut(s)	530, 2187
MslI	CAYNNNNRTG	2 cut(s)	324, 2949
MspA1I	CMGCKG	3 cut(s)	308, 479, 2813
MspI	CCGG	4 cut(s)	45, 68, 2293, 3108
MspR9I	CCNGG	4 cut(s)	69, 93, 2018, 3108
MunI	CAATTG	1 cut(s)	2492
MvaI	CCWGG	2 cut(s)	93, 2018
MvnI	CGCG	1 cut(s)	1191
MwoI	GCNNNNNNNGC	6 cut(s)	305, 445, 458, 1735, 1834, 2255
NarI	GGCGCC	1 cut(s)	96
NciI	CCSGG	2 cut(s)	69, 3108
NlaIV	GGNNCC	6 cut(s)	90, 97, 2015, 2283, 2749, 3078
NmeAIII	GCCGAG	1 cut(s)	63
NmuCI	GTSAC	4 cut(s)	1058, 2681, 2986, 3110
NsiI	ATGCAT	1 cut(s)	441
NspI	RCATGY	2 cut(s)	560, 2903
PaeI	GCATGC	1 cut(s)	560
PagI	TCATGA	2 cut(s)	1782, 2944
PaqCI	CACCTGC	1 cut(s)	2620
PciSI	GCTCTTC	1 cut(s)	1620
PcsI	WCGNNNNNNNCGW	1 cut(s)	595
PdmI	GAANNNNTTC	2 cut(s)	530, 2187
PkrI	GCNGC	8 cut(s)	178, 337, 447, 478, 562, 747, 1632, 2236
PleI	GAGTC	2 cut(s)	2053, 3178
PluTI	GGCGCC	1 cut(s)	99
PpsI	GAGTC	2 cut(s)	2053, 3178
PpuMI	RGGWCCY	1 cut(s)	1398
PshAI	GACNNNNGTC	1 cut(s)	3177
PshBI	ATTAAT	2 cut(s)	936, 1607
Psp124BI	GAGCTC	1 cut(s)	24
Psp5II	RGGWCCY	1 cut(s)	1398
Psp6I	CCWGG	2 cut(s)	91, 2016
PspEI	GGTNACC	1 cut(s)	2681
PspGI	CCWGG	2 cut(s)	91, 2016
PspN4I	GGNNCC	6 cut(s)	90, 97, 2015, 2283, 2749, 3078
PspOMI	GGGCCC	1 cut(s)	88
PspPI	GGNCC	6 cut(s)	88, 89, 238, 1398, 2014, 2747
PspPPI	RGGWCCY	1 cut(s)	1398
PstI	CTGCAG	1 cut(s)	340
PstNI	CAGNNNCTG	3 cut(s)	458, 826, 2549
PsuI	RGATCY	2 cut(s)	1195, 1489
PvuII	CAGCTG	1 cut(s)	479
RsaI	GTAC	3 cut(s)	1807, 2334, 2965
RsaNI	GTAC	3 cut(s)	1806, 2333, 2964
RseI	CAYNNNNRTG	2 cut(s)	324, 2949
SacI	GAGCTC	1 cut(s)	24
SapI	GCTCTTC	1 cut(s)	1620
SatI	GCNGC	8 cut(s)	177, 336, 446, 477, 561, 746, 1631, 2235
Sau96I	GGNCC	6 cut(s)	88, 89, 238, 1398, 2014, 2747
SchI	GAGTC	2 cut(s)	2054, 3178
ScrFI	CCNGG	4 cut(s)	69, 93, 2018, 3108
SduI	GDGCHC	7 cut(s)	24, 55, 92, 889, 1534, 2251, 2673
SexAI	ACCWGGT	1 cut(s)	2016
SfcI	CTRYAG	3 cut(s)	336, 1050, 1572
SfoI	GGCGCC	1 cut(s)	97
SinI	GGWCC	2 cut(s)	1398, 2014
SmiMI	CAYNNNNRTG	2 cut(s)	324, 2949
SmlI	CTYRAG	8 cut(s)	707, 1493, 1705, 1988, 2042, 2834, 3123, 3186
SmoI	CTYRAG	8 cut(s)	707, 1493, 1705, 1988, 2042, 2834, 3123, 3186
SpeI	ACTAGT	1 cut(s)	1475
SphI	GCATGC	1 cut(s)	560
SsiI	CCGC	7 cut(s)	176, 306, 578, 1553, 1643, 1771, 2811
SspDI	GGCGCC	1 cut(s)	95
SspI	AATATT	1 cut(s)	868
SstI	GAGCTC	1 cut(s)	24
StyD4I	CCNGG	4 cut(s)	67, 91, 2016, 3106
StyI	CCWWGG	2 cut(s)	1401, 2840
TaiI	ACGT	4 cut(s)	858, 1113, 2070, 2452
TaqI	TCGA	5 cut(s)	155, 219, 814, 2114, 3143
TauI	GCSGC	1 cut(s)	179
TscAI	CASTG	6 cut(s)	541, 2639, 2918, 2931, 3046, 3182
TseFI	GTSAC	4 cut(s)	1058, 2681, 2986, 3110
TseI	GCWGC	7 cut(s)	335, 445, 476, 560, 745, 1630, 2234
Tsp45I	GTSAC	4 cut(s)	1058, 2681, 2986, 3110
TspGWI	ACGGA	5 cut(s)	178, 517, 689, 1115, 1882
TspRI	CASTG	6 cut(s)	541, 2639, 2918, 2931, 3046, 3182
VneI	GTGCAC	1 cut(s)	2247
VpaK11BI	GGWCC	2 cut(s)	1398, 2014
VspI	ATTAAT	2 cut(s)	936, 1607
XbaI	TCTAGA	2 cut(s)	2026, 2303
XceI	RCATGY	2 cut(s)	560, 2903
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3092
XmaJI	CCTAGG	1 cut(s)	2840
XmnI	GAANNNNTTC	2 cut(s)	530, 2187
Zsp2I	ATGCAT	1 cut(s)	441

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.