MD02G1194600.v1.1

leucine-rich repeat receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012855
Physical Location & Seq
Reverse (-)
18579950 .. 18583836
3887 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1194600.v1.1.491

Sequence Viewer

Length: 3048 bp
ATGCGGCTCCTTTTCTTCCTCCTCCTCCTCCTCCTCCACCACCACTCTCTCTCCGCCATCGCAATGTCCAACTACCGCGCCCTCCTCTCCCTCAAGTCCTCCATAACCTCCGACCCCAACTCCGCCCTTTCCACCTGGACCCCCACCACCAGCCACTGCACTTGGTCCGGCGTCACCTGCGACTCCTCCCGCCGCTACGTCACCTCCCTCGACCTCTCCGGCCTCGACCTCACCGGCACTCTCTCCCCTGACCTCGCCCACCTCCGCTTCCTCGCCAACCTCACCCTCGCCGATAACCAGTTCTCGGGTCCCATCCCGCCCGAGATCTCCGCCCTCTCCGGCCTCCGCCTCCTCAACCTCTCCAACAACGTCTTCAACACCACCTTCCCCCCCAAGCTCTCCAACCTCACCCGCCTCACCGTCCTTGACCTCTACAACAACAACCTCACGGGCGACCTCCCCGTCTCCGTCACCCACATGACCTCCCTCCGCCACTTGCATTTGGGCGGCAACTTCTTCTCCGGCCAGATCCCTCCCGAGTTCGGCCGCTTCCCATTCCTCGAGTACTTAGCCGTCTCCGGCAACGAACTCGGCGGCCCAATACCCCCCGAAATCGGCAACTTAACCACCTTGAAAGAGCTCTACATCGGCTACTTCAACAGCTACGAAGGCGGAATACCGCCCGAAATCGGAAACTTATCCAATCTCGTCCGCTTCGACGCCGCCAACTGTAACTTAACCGGAGAGGTCCCGCCGGAGCTGGGTCGACTGCAAAATGTCGACACCCTGTTTCTCCAGGTCAATGCTCTTTCCGGTTCGTTGACACCCGAGCTGGGTTACCTCAAGAGCTTGAAATCCATGGACTTATCCAACAACGTCTTTACCGGCGAGATTCCGGGTTCCTTTTCGGAGCTCAAGAACTTGACCCTTTTGAATCTCTTCCGAAACAGGCTCCACGGCGCCATTCCCGACTTTATCGGCGACTTGCCGGAGCTCCAGGTGTTGCAGCTCTGGGAGAACAACTTCACCGGCAGCATCCCTCAGGGTCTGGGAAAAAACGGGAAGCTCCAAATTCTCGACCTTTCGTCGAACAAGTTGACGGGAACTCTCCCTCCCGATATGTGTTTCGGGAACAATCTCCAGACGCTCATCACTCTGTCAAACTTCCTTTTCGGGCCGATTCCCGAGTCGCTTGGCCGGTGCGATTCGCTGAGTCGGATTCGAATGGGTGACAACTACCTCAACGGGTCGATTCCGAAAGGGCTGCTTAGTTTGCCCAAGCTTACACAAGTCGAGTTGCAGGATAATCTCTTAGACGGTTCGTTTCCCGAGACAGATTCAATCTCGGGGAATCTCGGCCAGATTAGTCTCTCCAATAACCGGCTTTCCGGGTCTCTTCCGCCCACCATCGGCAACTTCTCCGGCGTCCAGAAGCTTCTTCTCGACGGAAACAAGTTCTCGGGTCGAATTCCACCCGAGATTGGGCGATTACAGCAGCTATCCAAGATCGATTTCAAGCACAACAAGTTCATGGGTTCCATAACCCCCGAAATCAGCCATTGCAAGCTCTTGACATTCGTCGATCTCAGCCGAAACGAGCTCTCCGGTGAGATTCCGAAGGAGGTTACTGGTATGAGGATACTGAACTACCTCAACCTGTCGAGAAACCATCTTGTGGGCAGCATTCCGTCGTCTATATCCACAATGCAGAGCTTGACATCTGTGGACTTTTCGTATAACAATCTTTCGGGTTTGGTTCCGGGCACTGGTCAGTTCAGTTACTTCAATTACACTTCGTTTCTGGGAAACCCCGATCTCTGCGGTCCCTATTTGGTTCCCTGCAAAGACGGGGTTGCCAATGGCACTCACCAACCACATGTCAAGGGTGCACTCACTGCTTCCCTGAAGCTATTGCTTGTTATTGGGTTGCTTCTGTGCTCGATCGTATTCGCCGTTGCAGCGATAATCAAGGCCCGGTCGTTGAAGAAAGCAAGCGATTCCCGAGCTTGGAAACTGACTGCATTCCAACGGTTGGACTTCACGGTTGATGATGTGTTGGATTCGTTGAAGGAGGACAACATTATTGGGAAAGGAGGGGCTGGGATTGTGTACAAGGGAGCTATGCCTAGTGGTGACAATGTGGCTGTCAAAAGACTCCCTGCAATGAGCAGAGGCTCCTCCCACGACCACGGATTCAACGCTGAAATCCAAACTCTTGGTCGAATTCGACACAGGCACATTGTAAGACTATTGGGTTTCTGTTCGAATCACGAGACAAATCTTTTAGTCTACGAGTACATGCCCAATGGGAGCTTGGGTGAAGTTCTTCATGGTAAGAAAGGGGGTCACTTGCATTGGGACACAAGGTACAAAATCGCCATTGAGGCTGCAAAGGGTCTATGCTATCTTCACCACGACTGCTCGCCTTTGATCGTCCACAGAGACGTGAAGTCGAATAACATCCTTCTCGACTCCAATTTCGAAGCTCATGTTGCTGATTTCGGCCTTGCCAAGTTCCTGCAGGATTCCGGAACATCCGAATGCATGTCTGCAATTGCTGGTTCTTATGGATATATTGCTCCAGAGTATGCATACACATTGAAGGTTGATGAGAAGAGCGACGTTTATAGCTTCGGTGTGGTTCTCTTGGAGCTTGTTAGCGGCAGGAAACCGGTTGGTGAATTTGGGGACGGAGTAGACATAGTCCAATGGGTGCGAAAAATGACAGACTCAAACAAGGAAGGAGTCCTCAAAATCCTTGACCCTAGACTCCCTTCAGTTCCTCTCCACGAGGTTATGCACGTATTTTACGTTGCAATGCTCTGTGTGGAAGAACAAGCAGTGGAGCGTCCAACAATGCGAGAAGTGGTTCAAATTCTAACCGAGCTTCCAAAGGCACCTGGCTCAAAACTGGTAGAGGATTCAGCCATCACAGAGTCCTCCCCTCCCCCTGCCACTACTGCATCCGAGTCTCCAGGTACAACGTCAAAAGACACGAAAGACCAGCCACCACTGCAGTCTCCGCCGCCTGATCTTCTTAGCATATGA

Protein Analysis

1016

Amino Acids

110.56

Weight (kDa)

6.17

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 61 1.8e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 84 - 168 4.4e-06 Leucine-rich repeat region
LRR_8 PF13855 114 - 171 2.7e-06 Leucine rich repeat
LRR_4 PF12799 137 - 176 5.8e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 138 - 197 4.3e-06 Leucine rich repeat
LRR_14 PF23598 159 - 269 1.8e-07 Leucine-rich repeat region
LRR_8 PF13855 260 - 318 6.9e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 691 - 960 6.2e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 691 - 958 3.5e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012855)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49670 AT5G65700 AT5G65700
fragaria_vesca FvH4_1g18710
malus_domestica MD02G1194600.v1.1 MD15G1304900.v1.1
prunus_persica Prupe.6G212200_v2.0.a1
pyrus_communis pycom02g15850 pycom15g26730
rosa_chinensis RchiOBHm_Chr2g0109581
rosa_laevigata RLG00000017747
rosa_multiflora Rmu_sc0000332.1_g000045
rosa_roxburghii Rroxscaffold_2G00134130
rosa_rugosa Rorug02G0160800
rosa_samantha Rh2AG213200 Rh2BG223400 Rh2CG215200 Rh2DG218700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 185
AasI GACNNNNNNGTC 1 cut(s) 461
Acc36I ACCTGC 1 cut(s) 185
AccB1I GGYRCC 2 cut(s) 959, 2896
AccB7I CCANNNNNTGG 2 cut(s) 1675, 2032
AccI GTMKAC 4 cut(s) 766, 780, 2289, 2697
AccII CGCG 1 cut(s) 78
AccIII TCCGGA 1 cut(s) 2528
AclWI GGATC 1 cut(s) 523
AcoI YGGCCR 4 cut(s) 523, 544, 1195, 1357
AcsI RAATTY 5 cut(s) 1071, 1467, 2223, 2681, 2874
AcuI CTGAAG 2 cut(s) 1925, 2760
AcyI GRCGYC 4 cut(s) 171, 720, 960, 1425
AfaI GTAC 5 cut(s) 566, 2111, 2297, 2369, 2980
AflIII ACRYGT 1 cut(s) 1876
AgeI ACCGGT 1 cut(s) 2671
AhdI GACNNNNNGTC 2 cut(s) 1084, 2449
AjiI CACGTC 1 cut(s) 2446
AjnI CCWGG 5 cut(s) 134, 795, 996, 2899, 2974
AjuI GAANNNNNNNTTGG 4 cut(s) 356, 388, 1496, 1528
AloI GAACNNNNNNTCC 2 cut(s) 1096, 1128
Alw21I GWGCWC 6 cut(s) 642, 915, 996, 1602, 1891, 1940
Alw26I GTCTC 9 cut(s) 469, 580, 1325, 1373, 1398, 2267, 2436, 2976, 3024
Alw44I GTGCAC 1 cut(s) 1887
AlwI GGATC 1 cut(s) 523
AlwNI CAGNNNCTG 2 cut(s) 156, 1048
Aor13HI TCCGGA 1 cut(s) 2528
ApaLI GTGCAC 1 cut(s) 1887
ApeKI GCWGC 7 cut(s) 1006, 1032, 1264, 1495, 1680, 1958, 2387
ApoI RAATTY 5 cut(s) 1071, 1467, 2223, 2681, 2874
ArsI GACNNNNNNTTYG 4 cut(s) 2203, 2235, 2462, 2494
AsiGI ACCGGT 1 cut(s) 2671
Asp700I GAANNNNTTC 4 cut(s) 938, 1022, 2325, 2868
AspLEI GCGC 2 cut(s) 80, 962
AspS9I GGNCC 8 cut(s) 138, 165, 308, 596, 748, 1175, 1823, 1972
AsuC2I CCSGG 4 cut(s) 897, 1390, 1761, 1975
AsuII TTCGAA 3 cut(s) 1222, 2264, 2481
AvaII GGWCC 5 cut(s) 138, 165, 308, 748, 1823
AxyI CCTNAGG 1 cut(s) 1041
BaeGI GKGCMC 2 cut(s) 1766, 1891
BanI GGYRCC 2 cut(s) 959, 2896
BanII GRGCYC 4 cut(s) 642, 915, 996, 1602
BarI GAAGNNNNNNTAC 2 cut(s) 660, 692
BauI CACGAG 2 cut(s) 2270, 2789
BbsI GAAGAC 1 cut(s) 364
Bbv12I GWGCWC 6 cut(s) 642, 915, 996, 1602, 1891, 1940
BbvI GCAGC 7 cut(s) 1018, 1044, 1251, 1507, 1692, 1970, 2374
BccI CCATC 5 cut(s) 65, 320, 1415, 1677, 2936
BceAI ACGGC 3 cut(s) 557, 973, 1937
BcgI CGANNNNNNTGC 4 cut(s) 571, 605, 1246, 1280
BciT130I CCWGG 5 cut(s) 136, 797, 998, 2901, 2976
BciVI GTATCC 1 cut(s) 1632
BcnI CCSGG 4 cut(s) 897, 1390, 1761, 1975
BcoDI GTCTC 9 cut(s) 469, 580, 1325, 1373, 1398, 2267, 2436, 2976, 3024
BfaI CTAG 2 cut(s) 2127, 2766
BfmI CTRYAG 2 cut(s) 2519, 3014
BfoI RGCGCY 1 cut(s) 963
BfuAI ACCTGC 1 cut(s) 185
BfuI GTATCC 1 cut(s) 1632
BglI GCCNNNNNGGC 1 cut(s) 2384
BglII AGATCT 1 cut(s) 324
BmcAI AGTACT 1 cut(s) 566
Bme1390I CCNGG 9 cut(s) 136, 797, 897, 998, 1390, 1761, 1975, 2901, 2976
Bme18I GGWCC 5 cut(s) 138, 165, 308, 748, 1823
BmeRI GACNNNNNGTC 2 cut(s) 1084, 2449
BmgBI CACGTC 1 cut(s) 2446
BmgT120I GGNCC 8 cut(s) 138, 165, 308, 596, 748, 1175, 1823, 1972
BmrFI CCNGG 9 cut(s) 136, 797, 897, 998, 1390, 1761, 1975, 2901, 2976
BmsI GCATC 2 cut(s) 1044, 2972
BoxI GACNNNNGTC 1 cut(s) 1577
BpiI GAAGAC 1 cut(s) 364
BpmI CTGGAG 5 cut(s) 779, 980, 1124, 2565, 2958
Bpu14I TTCGAA 3 cut(s) 1222, 2264, 2481
BpuEI CTTGAG 3 cut(s) 77, 827, 899
BpuMI CCSGG 4 cut(s) 897, 1390, 1761, 1975
Bsa29I ATCGAT 1 cut(s) 1509
BsaAI YACGTR 1 cut(s) 2803
BsaHI GRCGYC 4 cut(s) 171, 720, 960, 1425
BsaI GGTCTC 1 cut(s) 1398
BsaJI CCNNGG 3 cut(s) 858, 955, 2188
BsaWI WCCGGW 5 cut(s) 740, 812, 1604, 2528, 2671
Bse118I RCCGGY 6 cut(s) 233, 884, 1028, 1197, 1380, 2671
Bse1I ACTGG 4 cut(s) 298, 1633, 1771, 2916
Bse21I CCTNAGG 1 cut(s) 1041
Bse3DI GCAATG 4 cut(s) 69, 1558, 2169, 2823
BseAI TCCGGA 1 cut(s) 2528
BseBI CCWGG 5 cut(s) 136, 797, 998, 2901, 2976
BseCI ATCGAT 1 cut(s) 1509
BseDI CCNNGG 3 cut(s) 858, 955, 2188
BseGI GGATG 5 cut(s) 312, 1035, 2460, 2534, 2963
BseMI GCAATG 4 cut(s) 69, 1558, 2169, 2823
BseMII CTCAG 3 cut(s) 1055, 1202, 1600
BseNI ACTGG 4 cut(s) 298, 1633, 1771, 2916
BseRI GAGGAG 9 cut(s) 11, 14, 17, 20, 23, 74, 175, 341, 2167
BseSI GKGCMC 2 cut(s) 1766, 1891
BseX3I CGGCCG 1 cut(s) 544
BseXI GCAGC 7 cut(s) 1018, 1044, 1251, 1507, 1692, 1970, 2374
BseYI CCCAGC 3 cut(s) 760, 832, 2099
BsgI GTGCAG 1 cut(s) 142
Bsh1236I CGCG 1 cut(s) 78
Bsh1285I CGRYCG 3 cut(s) 547, 1944, 1979
BshNI GGYRCC 2 cut(s) 959, 2896
BshTI ACCGGT 1 cut(s) 2671
BshVI ATCGAT 1 cut(s) 1509
BsiEI CGRYCG 3 cut(s) 547, 1944, 1979
BsiHKAI GWGCWC 6 cut(s) 642, 915, 996, 1602, 1891, 1940
BslFI GGGAC 5 cut(s) 294, 734, 1809, 2372, 2702
BsmAI GTCTC 9 cut(s) 469, 580, 1325, 1373, 1398, 2267, 2436, 2976, 3024
BsmBI CGTCTC 3 cut(s) 469, 580, 2436
BsmFI GGGAC 5 cut(s) 294, 734, 1809, 2372, 2702
BsmI GAATGC 3 cut(s) 1683, 2021, 2546
Bso31I GGTCTC 1 cut(s) 1398
Bsp119I TTCGAA 3 cut(s) 1222, 2264, 2481
Bsp1286I GDGCHC 7 cut(s) 642, 915, 996, 1602, 1766, 1891, 1940
Bsp13I TCCGGA 1 cut(s) 2528
Bsp1407I TGTACA 1 cut(s) 2109
Bsp143I GATC 8 cut(s) 324, 528, 1506, 1582, 1813, 1941, 2430, 3031
Bsp19I CCATGG 1 cut(s) 858
BspCNI CTCAG 3 cut(s) 1054, 1203, 1599
BspDI ATCGAT 1 cut(s) 1509
BspEI TCCGGA 1 cut(s) 2528
BspFNI CGCG 1 cut(s) 78
BspMAI CTGCAG 2 cut(s) 2523, 3018
BspMI ACCTGC 1 cut(s) 185
BspPI GGATC 1 cut(s) 523
BspQI GCTCTTC 1 cut(s) 2609
BspT104I TTCGAA 3 cut(s) 1222, 2264, 2481
BspT107I GGYRCC 2 cut(s) 959, 2896
BspTNI GGTCTC 1 cut(s) 1398
BsrDI GCAATG 4 cut(s) 69, 1558, 2169, 2823
BsrFI RCCGGY 6 cut(s) 233, 884, 1028, 1197, 1380, 2671
BsrGI TGTACA 1 cut(s) 2109
BsrI ACTGG 4 cut(s) 298, 1633, 1771, 2916
BssAI RCCGGY 6 cut(s) 233, 884, 1028, 1197, 1380, 2671
BssECI CCNNGG 3 cut(s) 858, 955, 2188
BssMI GATC 8 cut(s) 324, 528, 1506, 1582, 1813, 1941, 2430, 3031
BssNI GRCGYC 4 cut(s) 171, 720, 960, 1425
BssSI CACGAG 2 cut(s) 2270, 2789
BssT1I CCWWGG 1 cut(s) 858
Bst2BI CACGAG 2 cut(s) 2270, 2789
Bst2UI CCWGG 5 cut(s) 136, 797, 998, 2901, 2976
Bst4CI ACNGT 5 cut(s) 421, 731, 1319, 2031, 2044
Bst6I CTCTTC 3 cut(s) 944, 1401, 2609
BstACI GRCGYC 4 cut(s) 171, 720, 960, 1425
BstAPI GCANNNNNTGC 1 cut(s) 1895
BstAUI TGTACA 1 cut(s) 2109
BstBAI YACGTR 1 cut(s) 2803
BstBI TTCGAA 3 cut(s) 1222, 2264, 2481
BstC8I GCNNGC 3 cut(s) 1565, 1993, 2423
BstDEI CTNAG 7 cut(s) 568, 1041, 1211, 1268, 1312, 1586, 3038
BstDSI CCRYGG 3 cut(s) 858, 955, 2188
BstEII GGTNACC 1 cut(s) 836
BstF5I GGATG 5 cut(s) 312, 1035, 2460, 2534, 2963
BstFNI CGCG 1 cut(s) 78
BstH2I RGCGCY 1 cut(s) 963
BstHHI GCGC 2 cut(s) 80, 962
BstKTI GATC 8 cut(s) 327, 531, 1509, 1585, 1816, 1944, 2433, 3034
BstMAI GTCTC 9 cut(s) 469, 580, 1325, 1373, 1398, 2267, 2436, 2976, 3024
BstMBI GATC 8 cut(s) 324, 528, 1506, 1582, 1813, 1941, 2430, 3031
BstMCI CGRYCG 3 cut(s) 547, 1944, 1979
BstNI CCWGG 5 cut(s) 136, 797, 998, 2901, 2976
BstNSI RCATGY 3 cut(s) 1880, 2302, 2548
BstPAI GACNNNNGTC 1 cut(s) 1577
BstPI GGTNACC 1 cut(s) 836
BstSCI CCNGG 9 cut(s) 134, 795, 895, 996, 1388, 1759, 1973, 2899, 2974
BstSFI CTRYAG 2 cut(s) 2519, 3014
BstSLI GKGCMC 2 cut(s) 1766, 1891
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 7 cut(s) 1018, 1044, 1251, 1507, 1692, 1970, 2374
BstV2I GAAGAC 1 cut(s) 364
BstX2I RGATCY 2 cut(s) 324, 528
BstXI CCANNNNNNTGG 1 cut(s) 2216
BstYI RGATCY 2 cut(s) 324, 528
BstZI CGGCCG 1 cut(s) 544
Bsu15I ATCGAT 1 cut(s) 1509
Bsu36I CCTNAGG 1 cut(s) 1041
BsuI GTATCC 1 cut(s) 1632
BsuTUI ATCGAT 1 cut(s) 1509
BtgI CCRYGG 3 cut(s) 858, 955, 2188
BtgZI GCGATG 1 cut(s) 43
BtrI CACGTC 1 cut(s) 2446
BtsCI GGATG 5 cut(s) 312, 1035, 2460, 2534, 2963
BtsI GCAGTG 4 cut(s) 154, 1893, 2847, 3011
BtsIMutI CAGTG 5 cut(s) 154, 1764, 1893, 2847, 3011
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 3 cut(s) 1565, 1993, 2423
CaiI CAGNNNCTG 2 cut(s) 156, 1048
CfoI GCGC 2 cut(s) 80, 962
Cfr10I RCCGGY 6 cut(s) 233, 884, 1028, 1197, 1380, 2671
Cfr13I GGNCC 8 cut(s) 138, 165, 308, 596, 748, 1175, 1823, 1972
ClaI ATCGAT 1 cut(s) 1509
CseI GACGC 5 cut(s) 160, 728, 1153, 1414, 2837
Csp6I GTAC 5 cut(s) 565, 2110, 2296, 2368, 2979
CspAI ACCGGT 1 cut(s) 2671
CspCI CAANNNNNGTGG 2 cut(s) 143, 178
CviAII CATG 8 cut(s) 478, 859, 1531, 1877, 2299, 2330, 2489, 2545
CviQI GTAC 5 cut(s) 565, 2110, 2296, 2368, 2979
DdeI CTNAG 7 cut(s) 568, 1041, 1211, 1268, 1312, 1586, 3038
DinI GGCGCC 1 cut(s) 961
DpnI GATC 8 cut(s) 326, 530, 1508, 1584, 1815, 1943, 2432, 3033
DpnII GATC 8 cut(s) 324, 528, 1506, 1582, 1813, 1941, 2430, 3031
DrdI GACNNNNNNGTC 1 cut(s) 461
DriI GACNNNNNGTC 2 cut(s) 1084, 2449
DseDI GACNNNNNNGTC 1 cut(s) 461
EaeI YGGCCR 4 cut(s) 523, 544, 1195, 1357
EagI CGGCCG 1 cut(s) 544
Eam1104I CTCTTC 3 cut(s) 944, 1401, 2609
Eam1105I GACNNNNNGTC 2 cut(s) 1084, 2449
EarI CTCTTC 3 cut(s) 944, 1401, 2609
EciI GGCGGA 8 cut(s) 43, 112, 319, 335, 479, 687, 1389, 3012
Ecl136II GAGCTC 4 cut(s) 640, 913, 994, 1600
EclXI CGGCCG 1 cut(s) 544
Eco130I CCWWGG 1 cut(s) 858
Eco24I GRGCYC 4 cut(s) 642, 915, 996, 1602
Eco31I GGTCTC 1 cut(s) 1398
Eco47I GGWCC 5 cut(s) 138, 165, 308, 748, 1823
Eco52I CGGCCG 1 cut(s) 544
Eco53kI GAGCTC 4 cut(s) 640, 913, 994, 1600
Eco57I CTGAAG 2 cut(s) 1925, 2760
Eco81I CCTNAGG 1 cut(s) 1041
Eco91I GGTNACC 1 cut(s) 836
EcoICRI GAGCTC 4 cut(s) 640, 913, 994, 1600
EcoO109I RGGNCCY 2 cut(s) 308, 748
EcoO65I GGTNACC 1 cut(s) 836
EcoRI GAATTC 2 cut(s) 1467, 2223
EcoRII CCWGG 5 cut(s) 134, 795, 996, 2899, 2974
EcoT14I CCWWGG 1 cut(s) 858
EcoT22I ATGCAT 2 cut(s) 2546, 2593
EcoT38I GRGCYC 4 cut(s) 642, 915, 996, 1602
EgeI GGCGCC 1 cut(s) 961
EheI GGCGCC 1 cut(s) 961
ErhI CCWWGG 1 cut(s) 858
Esp3I CGTCTC 3 cut(s) 469, 580, 2436
FaeI CATG 8 cut(s) 481, 862, 1534, 1880, 2302, 2333, 2492, 2548
FalI AAGNNNNNCTT 2 cut(s) 1251, 1283
FaqI GGGAC 5 cut(s) 294, 734, 1809, 2372, 2702
FatI CATG 8 cut(s) 477, 858, 1530, 1876, 2298, 2329, 2488, 2544
FauI CCCGC 4 cut(s) 197, 324, 419, 759
FauNDI CATATG 1 cut(s) 3044
FblI GTMKAC 4 cut(s) 766, 780, 2289, 2697
FokI GGATG 5 cut(s) 299, 1022, 2447, 2521, 2950
FriOI GRGCYC 4 cut(s) 642, 915, 996, 1602
FspBI CTAG 2 cut(s) 2127, 2766
GlaI GCGC 2 cut(s) 79, 961
GsaI CCCAGC 3 cut(s) 764, 836, 2103
GsuI CTGGAG 5 cut(s) 779, 980, 1124, 2565, 2958
HaeII RGCGCY 1 cut(s) 963
HgaI GACGC 5 cut(s) 160, 728, 1153, 1414, 2837
HhaI GCGC 2 cut(s) 80, 962
Hin1I GRCGYC 4 cut(s) 171, 720, 960, 1425
Hin1II CATG 8 cut(s) 481, 862, 1534, 1880, 2302, 2333, 2492, 2548
Hin6I GCGC 2 cut(s) 78, 960
HinP1I GCGC 2 cut(s) 78, 960
HincII GTYRAC 4 cut(s) 767, 781, 822, 1098
HindII GTYRAC 4 cut(s) 767, 781, 822, 1098
HindIII AAGCTT 2 cut(s) 1280, 1433
Hpy188I TCNGA 9 cut(s) 112, 692, 910, 944, 1218, 1257, 1617, 2539, 2968
Hpy99I CGWCG 6 cut(s) 722, 1090, 1448, 1583, 1693, 2624
HpyAV CCTTC 8 cut(s) 394, 662, 1612, 2062, 2474, 2596, 2735, 2784
HpyCH4III ACNGT 5 cut(s) 421, 731, 1319, 2031, 2044
HpyCH4IV ACGT 8 cut(s) 198, 369, 876, 2445, 2622, 2802, 2811, 2984
HpyF3I CTNAG 7 cut(s) 568, 1041, 1211, 1268, 1312, 1586, 3038
HpySE526I ACGT 8 cut(s) 198, 369, 876, 2445, 2622, 2802, 2811, 2984
Hsp92I GRCGYC 4 cut(s) 171, 720, 960, 1425
Hsp92II CATG 8 cut(s) 481, 862, 1534, 1880, 2302, 2333, 2492, 2548
HspAI GCGC 2 cut(s) 78, 960
KasI GGCGCC 1 cut(s) 959
KflI GGGWCCC 1 cut(s) 308
Kpn2I TCCGGA 1 cut(s) 2528
Kzo9I GATC 8 cut(s) 324, 528, 1506, 1582, 1813, 1941, 2430, 3031
LguI GCTCTTC 1 cut(s) 2609
Lsp1109I GCAGC 7 cut(s) 1018, 1044, 1251, 1507, 1692, 1970, 2374
LweI GCATC 2 cut(s) 1044, 2972
MaeI CTAG 2 cut(s) 2127, 2766
MaeII ACGT 8 cut(s) 198, 369, 876, 2445, 2622, 2802, 2811, 2984
MalI GATC 8 cut(s) 326, 530, 1508, 1584, 1815, 1943, 2432, 3033
MboI GATC 8 cut(s) 324, 528, 1506, 1582, 1813, 1941, 2430, 3031
MfeI CAATTG 1 cut(s) 2553
MflI RGATCY 2 cut(s) 324, 528
MhlI GDGCHC 7 cut(s) 642, 915, 996, 1602, 1766, 1891, 1940
MluCI AATT 8 cut(s) 1071, 1467, 1786, 2223, 2476, 2553, 2681, 2874
Mly113I GGCGCC 1 cut(s) 960
Mph1103I ATGCAT 2 cut(s) 2546, 2593
MroI TCCGGA 1 cut(s) 2528
MroXI GAANNNNTTC 4 cut(s) 938, 1022, 2325, 2868
MseI TTAA 2 cut(s) 623, 737
MslI CAYNNNNRTG 3 cut(s) 62, 476, 2539
MspR9I CCNGG 9 cut(s) 136, 797, 897, 998, 1390, 1761, 1975, 2901, 2976
MunI CAATTG 1 cut(s) 2553
Mva1269I GAATGC 3 cut(s) 1683, 2021, 2546
MvaI CCWGG 5 cut(s) 136, 797, 998, 2901, 2976
MvnI CGCG 1 cut(s) 78
NarI GGCGCC 1 cut(s) 960
NciI CCSGG 4 cut(s) 897, 1390, 1761, 1975
NcoI CCATGG 1 cut(s) 858
NdeI CATATG 1 cut(s) 3044
NdeII GATC 8 cut(s) 324, 528, 1506, 1582, 1813, 1941, 2430, 3031
NlaIII CATG 8 cut(s) 481, 862, 1534, 1880, 2302, 2333, 2492, 2548
NmeAIII GCCGAG 2 cut(s) 570, 1335
NmuCI GTSAC 6 cut(s) 172, 199, 469, 1229, 2132, 2345
NsiI ATGCAT 2 cut(s) 2546, 2593
NspI RCATGY 3 cut(s) 1880, 2302, 2548
NspV TTCGAA 3 cut(s) 1222, 2264, 2481
PaeR7I CTCGAG 1 cut(s) 560
PaqCI CACCTGC 1 cut(s) 185
PciI ACATGT 1 cut(s) 1876
PciSI GCTCTTC 1 cut(s) 2609
PcsI WCGNNNNNNNCGW 3 cut(s) 714, 1950, 2808
PctI GAATGC 3 cut(s) 1683, 2021, 2546
PdmI GAANNNNTTC 4 cut(s) 938, 1022, 2325, 2868
PflFI GACNNNGTC 1 cut(s) 2702
PflMI CCANNNNNTGG 2 cut(s) 1675, 2032
PinAI ACCGGT 1 cut(s) 2671
Ple19I CGATCG 1 cut(s) 1944
PluTI GGCGCC 1 cut(s) 963
Ppu21I YACGTR 1 cut(s) 2803
PpuMI RGGWCCY 2 cut(s) 308, 748
PscI ACATGT 1 cut(s) 1876
PshAI GACNNNNGTC 1 cut(s) 1577
Psp124BI GAGCTC 4 cut(s) 642, 915, 996, 1602
Psp5II RGGWCCY 2 cut(s) 308, 748
Psp6I CCWGG 5 cut(s) 134, 795, 996, 2899, 2974
PspEI GGTNACC 1 cut(s) 836
PspFI CCCAGC 3 cut(s) 760, 832, 2099
PspGI CCWGG 5 cut(s) 134, 795, 996, 2899, 2974
PspPI GGNCC 8 cut(s) 138, 165, 308, 596, 748, 1175, 1823, 1972
PspPPI RGGWCCY 2 cut(s) 308, 748
PspXI VCTCGAGB 1 cut(s) 560
PstI CTGCAG 2 cut(s) 2523, 3018
PstNI CAGNNNCTG 2 cut(s) 156, 1048
PsuI RGATCY 2 cut(s) 324, 528
PsyI GACNNNGTC 1 cut(s) 2702
PvuI CGATCG 1 cut(s) 1944
RsaI GTAC 5 cut(s) 566, 2111, 2297, 2369, 2980
RsaNI GTAC 5 cut(s) 565, 2110, 2296, 2368, 2979
RseI CAYNNNNRTG 3 cut(s) 62, 476, 2539
SacI GAGCTC 4 cut(s) 642, 915, 996, 1602
SalI GTCGAC 2 cut(s) 765, 779
SapI GCTCTTC 1 cut(s) 2609
SaqAI TTAA 2 cut(s) 623, 737
Sau3AI GATC 8 cut(s) 324, 528, 1506, 1582, 1813, 1941, 2430, 3031
Sau96I GGNCC 8 cut(s) 138, 165, 308, 596, 748, 1175, 1823, 1972
SbfI CCTGCAGG 1 cut(s) 2523
ScaI AGTACT 1 cut(s) 566
ScrFI CCNGG 9 cut(s) 136, 797, 897, 998, 1390, 1761, 1975, 2901, 2976
SdaI CCTGCAGG 1 cut(s) 2523
SduI GDGCHC 7 cut(s) 642, 915, 996, 1602, 1766, 1891, 1940
SfaNI GCATC 2 cut(s) 1044, 2972
SfcI CTRYAG 2 cut(s) 2519, 3014
SfoI GGCGCC 1 cut(s) 961
Sfr274I CTCGAG 1 cut(s) 560
SfuI TTCGAA 3 cut(s) 1222, 2264, 2481
SinI GGWCC 5 cut(s) 138, 165, 308, 748, 1823
SlaI CTCGAG 1 cut(s) 560
SmiMI CAYNNNNRTG 3 cut(s) 62, 476, 2539
SmlI CTYRAG 4 cut(s) 92, 560, 842, 914
SmoI CTYRAG 4 cut(s) 92, 560, 842, 914
Sse8387I CCTGCAGG 1 cut(s) 2523
Sse9I AATT 8 cut(s) 1071, 1467, 1786, 2223, 2476, 2553, 2681, 2874
SspDI GGCGCC 1 cut(s) 959
SspMI CTAG 2 cut(s) 2127, 2766
SstI GAGCTC 4 cut(s) 642, 915, 996, 1602
StyD4I CCNGG 9 cut(s) 134, 795, 895, 996, 1388, 1759, 1973, 2899, 2974
StyI CCWWGG 1 cut(s) 858
TaaI ACNGT 5 cut(s) 421, 731, 1319, 2031, 2044
TaiI ACGT 8 cut(s) 201, 372, 879, 2448, 2625, 2805, 2814, 2987
TasI AATT 8 cut(s) 1071, 1467, 1786, 2223, 2476, 2553, 2681, 2874
TatI WGTACW 3 cut(s) 564, 2109, 2295
TauI GCSGC 8 cut(s) 7, 195, 510, 549, 597, 725, 2664, 3028
Tru1I TTAA 2 cut(s) 623, 737
Tru9I TTAA 2 cut(s) 623, 737
TscAI CASTG 5 cut(s) 161, 1771, 1900, 2847, 3018
TseFI GTSAC 6 cut(s) 172, 199, 469, 1229, 2132, 2345
TseI GCWGC 7 cut(s) 1006, 1032, 1264, 1495, 1680, 1958, 2387
Tsp45I GTSAC 6 cut(s) 172, 199, 469, 1229, 2132, 2345
TspDTI ATGAA 2 cut(s) 1519, 2318
TspGWI ACGGA 5 cut(s) 457, 1461, 1677, 2205, 2706
TspRI CASTG 5 cut(s) 161, 1771, 1900, 2847, 3018
Tth111I GACNNNGTC 1 cut(s) 2702
Van91I CCANNNNNTGG 2 cut(s) 1675, 2032
VneI GTGCAC 1 cut(s) 1887
VpaK11BI GGWCC 5 cut(s) 138, 165, 308, 748, 1823
XapI RAATTY 5 cut(s) 1071, 1467, 2223, 2681, 2874
XceI RCATGY 3 cut(s) 1880, 2302, 2548
XcmI CCANNNNNNNNNTGG 1 cut(s) 2311
XhoI CTCGAG 1 cut(s) 560
XmiI GTMKAC 4 cut(s) 766, 780, 2289, 2697
XmnI GAANNNNTTC 4 cut(s) 938, 1022, 2325, 2868
XspI CTAG 2 cut(s) 2127, 2766
ZrmI AGTACT 1 cut(s) 566
Zsp2I ATGCAT 2 cut(s) 2546, 2593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.