Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0012855

Physical Location & Seq

Genomic Coordinates Reverse (-)

21688459 .. 21693234

Gene Structure 4776 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG213200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3090 bp

ATGCGGCTCCTCCTTCTCCTCCTCCTCCTCCACCTCCGCCACTCTCTCTCCTCACCGCACCGTCTCCTCTCCACAGCCACAGCCAAAGCCACACGCCCAATGTCCACCTACCGGGCCCTCCTCTCCCTCAAGGCCTCCATCACCGACGACCCCGACTCCCGCCTCTCCTCCTGGACCCCCAACACCAGCCACTGCACCTGGGCAGGCGTCACGTGCGACTCCCGCCGTCACGTGACCTCCCTCGACCTTTCCGGCTTGAATCTCTCCGGCTATCTCTCCCCCGATATCGCCCGCCTTGTCTACCTCTCTAACCTTACTCTCGCCGACAACACGTTCTCGGGTCCTATCCCGCCCGAGATCTCGGCCCTCTCGGGGCTCCGACTCCTCAACCTCTCCAACAACGTCTTCAACCGCACGTTCCCGCCGGAGCTCTCCAACCTCACAAACCTCCGCGTGCTCGACCTCTACAACAACAACTTGACCGGAGAATTGCCGGTGTCAGTGACCCAGATGACCAACCTCAGGCACTTGCATTTGGGTGGCAACTTTTTCGAGGGTTCGATCCCGCCGGAGTACGGCCGGTTCCCGTTTCTCGAGTACTTGGCCGTCTCCGGGAACTCGATCACCGGCAAAATCCCCAAGGAGATCGGAAACCTGACCAGGCTCAAGGAGCTTTACATTGGCTACTACAACAGCTACGACGGCGGCATACCGGCCGAGATCGGGAACTTGACAGAACTCGTCCGGTTCGACGCCGCCAACTGCAATTTATCCGGCGAGATCCCGCCGGAACTGGGTCGGTTGCAAAATGTGGATACTCTGTTTCTCCAGGTGAATGCGCTATCCGGGTCATTGACGTCTGAGCTGGGCTACCTCAAAAGCTTGAAATCCATGGACTTATCCAACAACTTGTTCTCCGGCGAGATTCCGGTCTCTTTCGCAGAGCTAAAGAACCTGACGCTTCTCAATCTCTTCCGGAACCAGCTTCACGGCGCCATTCCCGACTTCATCGGTGACTTGCCGGAGCTCCAGGTTCTGCAGCTATGGGAGAACAACTTCACTGGGTCTATTCCTCAGGGTTTGGGGAGAAATGGGAAGCTTCAAATCCTGGACTTGTCTTCGAACAAATTGACAGGAGCTCTTCCTCCTGATATGTGCACAGGAAACAATCTTCAAACCCTCATCACTCTCGGCAACTTTCTCTTCGGTCCTATTCCAGAGTCACTCGGAGACTGCCAGTCACTCAGTCGGATTCGAATGGGTGAGAACTTTCTAAATGGGTCGATTCCTAAAGGTCTATTTGGGTTGCCCAAACTCACCCAAGTGGAGCTTCAAGACAATTTCTTAGGCGGTTCATTTCCAGAGTCTGATTCAATCTCTGGTGCTCTCGGTCAGATTAGTCTTTCCAACAATCGGTTATCCGGGCCCTTGCCGCCGACCATCGGAAACTTCTCCGGCGTCCAGAAGCTTCTCCTTGACGGGAACAAGTTCTCGGGTCGAATCCCGCCGGAGATTGGACGCCTGCAGCAACTGTCAAAGATTGATTTTAGCCACAACATGTTTACGGGTCCAATTGCGCCGGCGATCAGCCAATGCAAGCTGCTGACGTTTGTTGATCTCAGTCGGAATGATCTCTCCGGTGAGATTCCGAAAGAGATTACCGGTATGAGGATACTGAATTACCTCAATCTCTCGAGGAACCATCTTGTGGGGAACATCCCATCTTCCATTTCCTCAATGCAAAGCTTAACCTCGGTGGATTTTTCGTATAACAATCTTTCGGGTTTGGTTCCGGGGACTGGTCAGTTCAGCTACTTCAATTACACTTCGTTTTTGGGCAACTCTGATCTTTGTGGTCCCTATTTGGTTCCTTGCAAAGATGGAGTTGCAAATGGCACTCATCAGCCTCGCGTCAAAAGCTCATTCACTGCTTCATTGAAGCTATTGCTCGTTGTGGGGTTGCTACTCTGCTCCATCATATTTGCAGTTATTACAATAATCAAAGCTCGATCGTACAAGAGAGAGAGGGATTCCAGAGCTTGGAAGTTGACTGCATTCCAGCGCTTGGATTTCACTGTTGATGATGTCTTGGATTCACTGAAGGAAGACAACATTATAGGCAAAGGAGGTGCTGGGATTGTGTACAAAGGCGCTATGCCTAGTGGTGACAATGTGGCTGTGAAAAGACTCCCTGCAATGAGCAGAGGCTCATCTCATGATCATGGATTCAATGCCGAGATTCAGACTCTGGGTCGAATTCGACACCGTCACATTGTTAGATTGTTGGGTTTCTGTTCAAACCATGAGACAAATCTTCTGGTCTATGAGTACATGCCCAATGGGAGTTTGGGTGAGGTTCTTCATGGCAAGAAAGGAGGTCACTTGCACTGGGATACAAGGTACAAGATTGCTATTGAAGCTGCAAAGGGGCTTTGCTATCTTCACCATGACTGTTCGCCTCTGATAGTCCATAGAGATGTCAAATCGAATAACATTCTCCTCGACTCCAATTTTGAGGCTCATGTTGCAGATTTTGGCCTTGCCAAGTTCCTTCAGGATTCCGGCACGTCCGAGTGCATGTCTGCCATTGCTGGTTCTTATGGATACATCGCTCCAGAGTATGCTTACACACTGAAGGTCGATGAGAAGAGCGACGTCTATAGTTTCGGTGTAGTTCTCTTGGAACTTGTTAGTGGCAAGAAACCAGTTGGTGAATTTGGAGATGGAGTCGATATAGTCCAATGGGTTCGGAAAATGACGGATTCAAACAAGGAAGGAGTCCTGAAGATCCTTGATTCCAGACTCCCGTCTGTTCCCCTACATGAGGTTATGCACGTATTTTACGTTGCAATGCTCTGCGTTGAAGAACAAGCAGTGGAACGTCCCACCATGAGAGAAGTGGTTCAAATTCTTACAGAGCTTCCAATTCCACCAGGCTCAAAGCAAGGAGGAGACACAACAACAATCACAGACTCCCCTCAACAATCTGCCATTATTACTGCAATGGAATCACCAACTACCACAAAAGACACGAAAGACCATCAACAGCCACCGCCACAATCGCCACCGCCAGATCTTCTAAGCATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009908 GO:0010073 GO:0010075 GO:0016020 GO:0022414 GO:0032501 GO:0032502 GO:0033612 GO:0040008 GO:0043621 GO:0044464 GO:0048229 GO:0048367 GO:0048437 GO:0048507 GO:0048509 GO:0048608 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0061458 GO:0065007 GO:0071944 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1029

Amino Acids

112.52

Weight (kDa)

6.37

Isoelectric Point (pI)

36.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	38 - 73	3.9e-12	Leucine rich repeat N-terminal domain
LRR_14	PF23598	119 - 180	4.4e-07	Leucine-rich repeat region
LRR_4	PF12799	148 - 182	1.5e-06	Leucine Rich repeats (2 copies)
LRR_14	PF23598	174 - 279	1.7e-08	Leucine-rich repeat region
LRR_14	PF23598	284 - 420	5.7e-07	Leucine-rich repeat region
PK_Tyr_Ser-Thr	PF07714	702 - 971	1.7e-45	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	702 - 969	2.4e-44	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0012855)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49670 AT5G65700 AT5G65700
fragaria_vesca	FvH4_1g18710
malus_domestica	MD02G1194600.v1.1 MD15G1304900.v1.1
prunus_persica	Prupe.6G212200_v2.0.a1
pyrus_communis	pycom02g15850 pycom15g26730
rosa_chinensis	RchiOBHm_Chr2g0109581
rosa_laevigata	RLG00000017747
rosa_multiflora	Rmu_sc0000332.1_g000045
rosa_roxburghii	Rroxscaffold_2G00134130
rosa_rugosa	Rorug02G0160800
rosa_samantha	Rh2AG213200 Rh2BG223400 Rh2CG215200 Rh2DG218700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	860, 2658
AccB1I	GGYRCC	1 cut(s)	992
AccB7I	CCANNNNNTGG	3 cut(s)	1708, 2040, 2065
AccI	GTMKAC	1 cut(s)	300
AccII	CGCG	2 cut(s)	453, 1911
AccIII	TCCGGA	1 cut(s)	975
AclWI	GGATC	3 cut(s)	556, 775, 2782
AcoI	YGGCCR	3 cut(s)	577, 603, 714
AcsI	RAATTY	3 cut(s)	2256, 2714, 2907
AcuI	CTGAAG	4 cut(s)	2120, 2537, 2654, 2804
AcvI	CACGTG	2 cut(s)	213, 232
AcyI	GRCGYC	7 cut(s)	207, 753, 857, 993, 1458, 1519, 2655
AfaI	GTAC	6 cut(s)	575, 599, 2015, 2144, 2330, 2402
AfeI	AGCGCT	1 cut(s)	2063
AflIII	ACRYGT	2 cut(s)	330, 1557
AgeI	ACCGGT	1 cut(s)	1661
AhdI	GACNNNNNGTC	2 cut(s)	1237, 2250
AjiI	CACGTC	1 cut(s)	2568
AjnI	CCWGG	7 cut(s)	170, 197, 659, 828, 1029, 1107, 2932
AjuI	GAANNNNNNNTTGG	6 cut(s)	389, 421, 896, 928, 1314, 1346
AleI	CACNNNNGTG	1 cut(s)	1322
Alw21I	GWGCWC	6 cut(s)	432, 459, 1029, 1141, 1160, 1387
Alw26I	GTCTC	6 cut(s)	68, 613, 937, 1224, 2300, 2946
Alw44I	GTGCAC	1 cut(s)	1156
AlwI	GGATC	3 cut(s)	556, 775, 2782
AlwNI	CAGNNNCTG	5 cut(s)	192, 1036, 1367, 1531, 2248
Ama87I	CYCGRG	6 cut(s)	337, 353, 370, 593, 1492, 1693
Aor13HI	TCCGGA	1 cut(s)	975
Aor51HI	AGCGCT	1 cut(s)	2063
AoxI	GGCC	8 cut(s)	114, 132, 363, 577, 603, 714, 1424, 2536
ApaI	GGGCCC	2 cut(s)	118, 1428
ApaLI	GTGCAC	1 cut(s)	1156
ApeKI	GCWGC	4 cut(s)	1039, 1525, 1600, 2420
ApoI	RAATTY	3 cut(s)	2256, 2714, 2907
ArsI	GACNNNNNNTTYG	2 cut(s)	2495, 2527
AsiGI	ACCGGT	1 cut(s)	1661
Asp700I	GAANNNNTTC	3 cut(s)	1055, 1487, 2901
AspLEI	GCGC	5 cut(s)	841, 995, 1579, 2064, 2153
AsuC2I	CCSGG	5 cut(s)	113, 613, 847, 1423, 1794
AsuII	TTCGAA	2 cut(s)	1121, 1255
AvaI	CYCGRG	6 cut(s)	337, 353, 370, 593, 1492, 1693
AvaII	GGWCC	5 cut(s)	174, 341, 1208, 1568, 1856
AxyI	CCTNAGG	2 cut(s)	521, 1074
BaeGI	GKGCMC	3 cut(s)	118, 1160, 1428
BanI	GGYRCC	1 cut(s)	992
BanII	GRGCYC	6 cut(s)	118, 378, 432, 1029, 1141, 1428
BbrPI	CACGTG	2 cut(s)	213, 232
BbsI	GAAGAC	3 cut(s)	397, 1110, 2112
Bbv12I	GWGCWC	6 cut(s)	432, 459, 1029, 1141, 1160, 1387
BbvI	GCAGC	4 cut(s)	1051, 1537, 1587, 2407
BccI	CCATC	8 cut(s)	146, 1448, 1710, 1729, 1874, 1982, 2717, 3048
BceAI	ACGGC	5 cut(s)	210, 590, 592, 718, 1006
BcgI	CGANNNNNNTGC	2 cut(s)	532, 566
BciT130I	CCWGG	7 cut(s)	172, 199, 661, 830, 1031, 1109, 2934
BciVI	GTATCC	4 cut(s)	808, 1665, 2386, 2597
BclI	TGATCA	1 cut(s)	2218
BcnI	CCSGG	5 cut(s)	113, 613, 847, 1423, 1794
BcoDI	GTCTC	6 cut(s)	68, 613, 937, 1224, 2300, 2946
BfaI	CTAG	1 cut(s)	2160
BfmI	CTRYAG	3 cut(s)	1037, 1523, 2659
BfoI	RGCGCY	3 cut(s)	996, 2065, 2154
BfuI	GTATCC	4 cut(s)	808, 1665, 2386, 2597
BglII	AGATCT	2 cut(s)	357, 3073
BisI	GCNGC	8 cut(s)	5, 706, 756, 1040, 1433, 1526, 1601, 2421
BlsI	GCNGC	8 cut(s)	6, 707, 757, 1041, 1434, 1527, 1602, 2422
BmcAI	AGTACT	1 cut(s)	599
Bme18I	GGWCC	5 cut(s)	174, 341, 1208, 1568, 1856
BmeRI	GACNNNNNGTC	2 cut(s)	1237, 2250
BmeT110I	CYCGRG	6 cut(s)	337, 353, 370, 593, 1492, 1693
BmgBI	CACGTC	1 cut(s)	2568
BmrI	ACTGGG	3 cut(s)	803, 1071, 2398
BmuI	ACTGGG	3 cut(s)	803, 1071, 2398
BoxI	GACNNNNGTC	1 cut(s)	2806
BpiI	GAAGAC	3 cut(s)	397, 1110, 2112
BpmI	CTGGAG	3 cut(s)	812, 1013, 2598
Bpu14I	TTCGAA	2 cut(s)	1121, 1255
BpuEI	CTTGAG	2 cut(s)	113, 650
BpuMI	CCSGG	5 cut(s)	113, 613, 847, 1423, 1794
BsaAI	YACGTR	3 cut(s)	213, 232, 2836
BsaHI	GRCGYC	7 cut(s)	207, 753, 857, 993, 1458, 1519, 2655
BsaI	GGTCTC	1 cut(s)	937
BsaJI	CCNNGG	5 cut(s)	198, 639, 891, 1752, 1793
BsaWI	WCCGGW	6 cut(s)	482, 744, 928, 975, 1637, 1661
Bse118I	RCCGGY	6 cut(s)	493, 579, 626, 712, 1579, 1661
Bse1I	ACTGG	6 cut(s)	798, 1066, 1237, 1804, 2393, 2705
Bse21I	CCTNAGG	2 cut(s)	521, 1074
Bse3DI	GCAATG	4 cut(s)	2202, 2586, 2856, 3009
BseAI	TCCGGA	1 cut(s)	975
BseBI	CCWGG	7 cut(s)	172, 199, 661, 830, 1031, 1109, 2934
BseDI	CCNNGG	5 cut(s)	198, 639, 891, 1752, 1793
BseGI	GGATG	1 cut(s)	1716
BseMI	GCAATG	4 cut(s)	2202, 2586, 2856, 3009
BseMII	CTCAG	5 cut(s)	535, 852, 1088, 1258, 1633
BseNI	ACTGG	6 cut(s)	798, 1066, 1237, 1804, 2393, 2705
BseSI	GKGCMC	3 cut(s)	118, 1160, 1428
BseX3I	CGGCCG	2 cut(s)	577, 714
BseXI	GCAGC	4 cut(s)	1051, 1537, 1587, 2407
BseYI	CCCAGC	2 cut(s)	865, 2132
BsgI	GTGCAG	1 cut(s)	178
Bsh1236I	CGCG	2 cut(s)	453, 1911
Bsh1285I	CGRYCG	3 cut(s)	580, 717, 2012
BshFI	GGCC	8 cut(s)	116, 134, 365, 579, 605, 716, 1426, 2538
BshNI	GGYRCC	1 cut(s)	992
BshTI	ACCGGT	1 cut(s)	1661
BsiEI	CGRYCG	3 cut(s)	580, 717, 2012
BsiHKAI	GWGCWC	6 cut(s)	432, 459, 1029, 1141, 1160, 1387
BsiHKCI	CYCGRG	6 cut(s)	337, 353, 370, 593, 1492, 1693
BslFI	GGGAC	3 cut(s)	1810, 1842, 2868
BsmAI	GTCTC	6 cut(s)	68, 613, 937, 1224, 2300, 2946
BsmBI	CGTCTC	2 cut(s)	68, 613
BsmFI	GGGAC	3 cut(s)	1810, 1842, 2868
BsmI	GAATGC	2 cut(s)	841, 2054
BsnI	GGCC	8 cut(s)	116, 134, 365, 579, 605, 716, 1426, 2538
Bso31I	GGTCTC	1 cut(s)	937
BsoBI	CYCGRG	6 cut(s)	337, 353, 370, 593, 1492, 1693
Bsp119I	TTCGAA	2 cut(s)	1121, 1255
Bsp120I	GGGCCC	2 cut(s)	114, 1424
Bsp1286I	GDGCHC	9 cut(s)	118, 378, 432, 459, 1029, 1141, 1160, 1387, 1428
Bsp13I	TCCGGA	1 cut(s)	975
Bsp1407I	TGTACA	1 cut(s)	2142
Bsp19I	CCATGG	1 cut(s)	891
BspANI	GGCC	8 cut(s)	116, 134, 365, 579, 605, 716, 1426, 2538
BspCNI	CTCAG	5 cut(s)	534, 853, 1087, 1257, 1632
BspEI	TCCGGA	1 cut(s)	975
BspFNI	CGCG	2 cut(s)	453, 1911
BspHI	TCATGA	1 cut(s)	2215
BspMAI	CTGCAG	2 cut(s)	1041, 1527
BspPI	GGATC	3 cut(s)	556, 775, 2782
BspQI	GCTCTTC	2 cut(s)	1146, 2642
BspT104I	TTCGAA	2 cut(s)	1121, 1255
BspT107I	GGYRCC	1 cut(s)	992
BspTNI	GGTCTC	1 cut(s)	937
BsrDI	GCAATG	4 cut(s)	2202, 2586, 2856, 3009
BsrFI	RCCGGY	6 cut(s)	493, 579, 626, 712, 1579, 1661
BsrGI	TGTACA	1 cut(s)	2142
BsrI	ACTGG	6 cut(s)	798, 1066, 1237, 1804, 2393, 2705
BssAI	RCCGGY	6 cut(s)	493, 579, 626, 712, 1579, 1661
BssECI	CCNNGG	5 cut(s)	198, 639, 891, 1752, 1793
BssNI	GRCGYC	7 cut(s)	207, 753, 857, 993, 1458, 1519, 2655
BssT1I	CCWWGG	2 cut(s)	639, 891
Bst2UI	CCWGG	7 cut(s)	172, 199, 661, 830, 1031, 1109, 2934
Bst4CI	ACNGT	5 cut(s)	62, 1533, 2077, 2267, 2453
Bst6I	CTCTTC	4 cut(s)	977, 1146, 1208, 2642
BstACI	GRCGYC	7 cut(s)	207, 753, 857, 993, 1458, 1519, 2655
BstAUI	TGTACA	1 cut(s)	2142
BstBAI	YACGTR	3 cut(s)	213, 232, 2836
BstBI	TTCGAA	2 cut(s)	1121, 1255
BstC8I	GCNNGC	6 cut(s)	205, 292, 455, 1523, 1581, 1598
BstDEI	CTNAG	7 cut(s)	521, 861, 1074, 1244, 1345, 1619, 3080
BstDSI	CCRYGG	1 cut(s)	891
BstENI	CCTNNNNNAGG	1 cut(s)	2822
BstF5I	GGATG	1 cut(s)	1716
BstFNI	CGCG	2 cut(s)	453, 1911
BstH2I	RGCGCY	3 cut(s)	996, 2065, 2154
BstHHI	GCGC	5 cut(s)	841, 995, 1579, 2064, 2153
BstMAI	GTCTC	6 cut(s)	68, 613, 937, 1224, 2300, 2946
BstMCI	CGRYCG	3 cut(s)	580, 717, 2012
BstMWI	GCNNNNNNNGC	9 cut(s)	213, 222, 670, 702, 1432, 1917, 2417, 2525, 3061
BstNI	CCWGG	7 cut(s)	172, 199, 661, 830, 1031, 1109, 2934
BstNSI	RCATGY	3 cut(s)	1561, 2335, 2581
BstPAI	GACNNNNGTC	1 cut(s)	2806
BstSFI	CTRYAG	3 cut(s)	1037, 1523, 2659
BstSLI	GKGCMC	3 cut(s)	118, 1160, 1428
BstUI	CGCG	2 cut(s)	453, 1911
BstV1I	GCAGC	4 cut(s)	1051, 1537, 1587, 2407
BstV2I	GAAGAC	3 cut(s)	397, 1110, 2112
BstX2I	RGATCY	4 cut(s)	357, 780, 2787, 3073
BstYI	RGATCY	4 cut(s)	357, 780, 2787, 3073
BstZI	CGGCCG	2 cut(s)	577, 714
Bsu36I	CCTNAGG	2 cut(s)	521, 1074
BsuI	GTATCC	4 cut(s)	808, 1665, 2386, 2597
BsuRI	GGCC	8 cut(s)	116, 134, 365, 579, 605, 716, 1426, 2538
BtgI	CCRYGG	1 cut(s)	891
BtgZI	GCGATG	1 cut(s)	2593
BtrI	CACGTC	1 cut(s)	2568
BtsCI	GGATG	1 cut(s)	1716
BtsI	GCAGTG	3 cut(s)	190, 1926, 2880
BtsIMutI	CAGTG	9 cut(s)	190, 507, 1059, 1926, 2073, 2096, 2386, 2630, 2880
Cac8I	GCNNGC	6 cut(s)	205, 292, 455, 1523, 1581, 1598
CaiI	CAGNNNCTG	5 cut(s)	192, 1036, 1367, 1531, 2248
CciI	TCATGA	1 cut(s)	2215
CfoI	GCGC	5 cut(s)	841, 995, 1579, 2064, 2153
Cfr10I	RCCGGY	6 cut(s)	493, 579, 626, 712, 1579, 1661
CseI	GACGC	6 cut(s)	196, 761, 967, 1447, 1527, 1900
Csp6I	GTAC	6 cut(s)	574, 598, 2014, 2143, 2329, 2401
CspAI	ACCGGT	1 cut(s)	1661
CviQI	GTAC	6 cut(s)	574, 598, 2014, 2143, 2329, 2401
DdeI	CTNAG	7 cut(s)	521, 861, 1074, 1244, 1345, 1619, 3080
DinI	GGCGCC	1 cut(s)	994
DriI	GACNNNNNGTC	2 cut(s)	1237, 2250
EaeI	YGGCCR	3 cut(s)	577, 603, 714
EagI	CGGCCG	2 cut(s)	577, 714
Eam1104I	CTCTTC	4 cut(s)	977, 1146, 1208, 2642
Eam1105I	GACNNNNNGTC	2 cut(s)	1237, 2250
EarI	CTCTTC	4 cut(s)	977, 1146, 1208, 2642
EciI	GGCGGA	1 cut(s)	26
Ecl136II	GAGCTC	3 cut(s)	430, 1027, 1139
EclXI	CGGCCG	2 cut(s)	577, 714
Eco130I	CCWWGG	2 cut(s)	639, 891
Eco147I	AGGCCT	1 cut(s)	134
Eco24I	GRGCYC	6 cut(s)	118, 378, 432, 1029, 1141, 1428
Eco31I	GGTCTC	1 cut(s)	937
Eco32I	GATATC	1 cut(s)	286
Eco47I	GGWCC	5 cut(s)	174, 341, 1208, 1568, 1856
Eco47III	AGCGCT	1 cut(s)	2063
Eco52I	CGGCCG	2 cut(s)	577, 714
Eco53kI	GAGCTC	3 cut(s)	430, 1027, 1139
Eco57I	CTGAAG	4 cut(s)	2120, 2537, 2654, 2804
Eco72I	CACGTG	2 cut(s)	213, 232
Eco81I	CCTNAGG	2 cut(s)	521, 1074
Eco88I	CYCGRG	6 cut(s)	337, 353, 370, 593, 1492, 1693
EcoICRI	GAGCTC	3 cut(s)	430, 1027, 1139
EcoNI	CCTNNNNNAGG	1 cut(s)	2822
EcoO109I	RGGNCCY	3 cut(s)	115, 341, 1425
EcoRI	GAATTC	1 cut(s)	2256
EcoRII	CCWGG	7 cut(s)	170, 197, 659, 828, 1029, 1107, 2932
EcoRV	GATATC	1 cut(s)	286
EcoT14I	CCWWGG	2 cut(s)	639, 891
EcoT38I	GRGCYC	6 cut(s)	118, 378, 432, 1029, 1141, 1428
EgeI	GGCGCC	1 cut(s)	994
EheI	GGCGCC	1 cut(s)	994
ErhI	CCWWGG	2 cut(s)	639, 891
Esp3I	CGTCTC	2 cut(s)	68, 613
FalI	AAGNNNNNCTT	2 cut(s)	1314, 1346
FaqI	GGGAC	3 cut(s)	1810, 1842, 2868
FauI	CCCGC	8 cut(s)	167, 230, 299, 357, 429, 573, 792, 1512
FbaI	TGATCA	1 cut(s)	2218
FblI	GTMKAC	1 cut(s)	300
Fnu4HI	GCNGC	8 cut(s)	5, 706, 756, 1040, 1433, 1526, 1601, 2421
FokI	GGATG	1 cut(s)	1703
FriOI	GRGCYC	6 cut(s)	118, 378, 432, 1029, 1141, 1428
Fsp4HI	GCNGC	8 cut(s)	5, 706, 756, 1040, 1433, 1526, 1601, 2421
FspBI	CTAG	1 cut(s)	2160
GlaI	GCGC	5 cut(s)	840, 994, 1578, 2063, 2152
GluI	GCNGC	8 cut(s)	5, 706, 756, 1040, 1433, 1526, 1601, 2421
GsaI	CCCAGC	2 cut(s)	869, 2136
GsuI	CTGGAG	3 cut(s)	812, 1013, 2598
HaeII	RGCGCY	3 cut(s)	996, 2065, 2154
HaeIII	GGCC	8 cut(s)	116, 134, 365, 579, 605, 716, 1426, 2538
HgaI	GACGC	6 cut(s)	196, 761, 967, 1447, 1527, 1900
HhaI	GCGC	5 cut(s)	841, 995, 1579, 2064, 2153
Hin1I	GRCGYC	7 cut(s)	207, 753, 857, 993, 1458, 1519, 2655
Hin6I	GCGC	5 cut(s)	839, 993, 1577, 2062, 2151
HinP1I	GCGC	5 cut(s)	839, 993, 1577, 2062, 2151
HincII	GTYRAC	1 cut(s)	2049
HindII	GTYRAC	1 cut(s)	2049
HindIII	AAGCTT	4 cut(s)	880, 1097, 1466, 1744
Hpy166II	GTNNAC	6 cut(s)	105, 301, 1158, 1563, 2049, 2143
Hpy8I	GTNNAC	6 cut(s)	105, 301, 1158, 1563, 2049, 2143
Hpy99I	CGWCG	4 cut(s)	149, 704, 755, 2657
HpyAV	CCTTC	5 cut(s)	23, 2095, 2561, 2629, 2768
HpyCH4III	ACNGT	5 cut(s)	62, 1533, 2077, 2267, 2453
HpyF10VI	GCNNNNNNNGC	9 cut(s)	213, 222, 670, 702, 1432, 1917, 2417, 2525, 3061
HpyF3I	CTNAG	7 cut(s)	521, 861, 1074, 1244, 1345, 1619, 3080
Hsp92I	GRCGYC	7 cut(s)	207, 753, 857, 993, 1458, 1519, 2655
HspAI	GCGC	5 cut(s)	839, 993, 1577, 2062, 2151
KasI	GGCGCC	1 cut(s)	992
Kpn2I	TCCGGA	1 cut(s)	975
KroI	GCCGGC	1 cut(s)	1579
KroNI	GCCGGC	1 cut(s)	1581
Ksp22I	TGATCA	1 cut(s)	2218
LguI	GCTCTTC	2 cut(s)	1146, 2642
Lsp1109I	GCAGC	4 cut(s)	1051, 1537, 1587, 2407
MaeI	CTAG	1 cut(s)	2160
MfeI	CAATTG	1 cut(s)	1572
MflI	RGATCY	4 cut(s)	357, 780, 2787, 3073
MhlI	GDGCHC	9 cut(s)	118, 378, 432, 459, 1029, 1141, 1160, 1387, 1428
Mly113I	GGCGCC	1 cut(s)	993
MmeI	TCCRAC	7 cut(s)	403, 420, 459, 927, 1229, 1431, 1604
MreI	CGCCGGCG	1 cut(s)	1579
MroI	TCCGGA	1 cut(s)	975
MroNI	GCCGGC	1 cut(s)	1579
MroXI	GAANNNNTTC	3 cut(s)	1055, 1487, 2901
MseI	TTAA	1 cut(s)	1748
MslI	CAYNNNNRTG	4 cut(s)	537, 1322, 2220, 2475
MunI	CAATTG	1 cut(s)	1572
Mva1269I	GAATGC	2 cut(s)	841, 2054
MvaI	CCWGG	7 cut(s)	172, 199, 661, 830, 1031, 1109, 2934
MvnI	CGCG	2 cut(s)	453, 1911
MwoI	GCNNNNNNNGC	9 cut(s)	213, 222, 670, 702, 1432, 1917, 2417, 2525, 3061
NaeI	GCCGGC	1 cut(s)	1581
NarI	GGCGCC	1 cut(s)	993
NciI	CCSGG	5 cut(s)	113, 613, 847, 1423, 1794
NcoI	CCATGG	1 cut(s)	891
NgoMIV	GCCGGC	1 cut(s)	1579
NmeAIII	GCCGAG	4 cut(s)	341, 742, 1170, 2260
NspI	RCATGY	3 cut(s)	1561, 2335, 2581
NspV	TTCGAA	2 cut(s)	1121, 1255
OliI	CACNNNNGTG	1 cut(s)	1322
PaeR7I	CTCGAG	2 cut(s)	593, 1693
PagI	TCATGA	1 cut(s)	2215
PceI	AGGCCT	1 cut(s)	134
PciI	ACATGT	1 cut(s)	1557
PciSI	GCTCTTC	2 cut(s)	1146, 2642
PcsI	WCGNNNNNNNCGW	2 cut(s)	747, 2841
PctI	GAATGC	2 cut(s)	841, 2054
PdiI	GCCGGC	1 cut(s)	1581
PdmI	GAANNNNTTC	3 cut(s)	1055, 1487, 2901
PflFI	GACNNNGTC	1 cut(s)	2265
PflMI	CCANNNNNTGG	3 cut(s)	1708, 2040, 2065
PfoI	TCCNGGA	3 cut(s)	170, 611, 1107
PinAI	ACCGGT	1 cut(s)	1661
PkrI	GCNGC	8 cut(s)	6, 707, 757, 1041, 1434, 1527, 1602, 2422
Ple19I	CGATCG	1 cut(s)	2012
PluTI	GGCGCC	1 cut(s)	996
PmaCI	CACGTG	2 cut(s)	213, 232
PmlI	CACGTG	2 cut(s)	213, 232
Ppu21I	YACGTR	3 cut(s)	213, 232, 2836
PpuMI	RGGWCCY	1 cut(s)	341
PscI	ACATGT	1 cut(s)	1557
PshAI	GACNNNNGTC	1 cut(s)	2806
Psp124BI	GAGCTC	3 cut(s)	432, 1029, 1141
Psp5II	RGGWCCY	1 cut(s)	341
Psp6I	CCWGG	7 cut(s)	170, 197, 659, 828, 1029, 1107, 2932
PspCI	CACGTG	2 cut(s)	213, 232
PspFI	CCCAGC	2 cut(s)	865, 2132
PspGI	CCWGG	7 cut(s)	170, 197, 659, 828, 1029, 1107, 2932
PspOMI	GGGCCC	2 cut(s)	114, 1424
PspPPI	RGGWCCY	1 cut(s)	341
PsrI	GAACNNNNNNTAC	2 cut(s)	566, 598
PstI	CTGCAG	2 cut(s)	1041, 1527
PstNI	CAGNNNCTG	5 cut(s)	192, 1036, 1367, 1531, 2248
PsuI	RGATCY	4 cut(s)	357, 780, 2787, 3073
PsyI	GACNNNGTC	1 cut(s)	2265
PvuI	CGATCG	1 cut(s)	2012
RsaI	GTAC	6 cut(s)	575, 599, 2015, 2144, 2330, 2402
RsaNI	GTAC	6 cut(s)	574, 598, 2014, 2143, 2329, 2401
RseI	CAYNNNNRTG	4 cut(s)	537, 1322, 2220, 2475
SacI	GAGCTC	3 cut(s)	432, 1029, 1141
SapI	GCTCTTC	2 cut(s)	1146, 2642
SaqAI	TTAA	1 cut(s)	1748
SatI	GCNGC	8 cut(s)	5, 706, 756, 1040, 1433, 1526, 1601, 2421
ScaI	AGTACT	1 cut(s)	599
SduI	GDGCHC	9 cut(s)	118, 378, 432, 459, 1029, 1141, 1160, 1387, 1428
SfcI	CTRYAG	3 cut(s)	1037, 1523, 2659
SfoI	GGCGCC	1 cut(s)	994
Sfr274I	CTCGAG	2 cut(s)	593, 1693
SfuI	TTCGAA	2 cut(s)	1121, 1255
SgrAI	CRCCGGYG	1 cut(s)	1579
SinI	GGWCC	5 cut(s)	174, 341, 1208, 1568, 1856
SlaI	CTCGAG	2 cut(s)	593, 1693
SmiMI	CAYNNNNRTG	4 cut(s)	537, 1322, 2220, 2475
SmlI	CTYRAG	4 cut(s)	128, 593, 665, 1693
SmoI	CTYRAG	4 cut(s)	128, 593, 665, 1693
SseBI	AGGCCT	1 cut(s)	134
SspDI	GGCGCC	1 cut(s)	992
SspMI	CTAG	1 cut(s)	2160
SstI	GAGCTC	3 cut(s)	432, 1029, 1141
StuI	AGGCCT	1 cut(s)	134
StyI	CCWWGG	2 cut(s)	639, 891
TaaI	ACNGT	5 cut(s)	62, 1533, 2077, 2267, 2453
TaqII	GACCGA	2 cut(s)	1196, 1379
TatI	WGTACW	3 cut(s)	597, 2142, 2328
TauI	GCSGC	4 cut(s)	7, 708, 758, 1435
Tru1I	TTAA	1 cut(s)	1748
Tru9I	TTAA	1 cut(s)	1748
TscAI	CASTG	9 cut(s)	197, 507, 1066, 1933, 2080, 2103, 2393, 2637, 2880
TseI	GCWGC	4 cut(s)	1039, 1525, 1600, 2420
TspDTI	ATGAA	4 cut(s)	997, 1344, 1923, 2351
TspGWI	ACGGA	1 cut(s)	2774
TspRI	CASTG	9 cut(s)	197, 507, 1066, 1933, 2080, 2103, 2393, 2637, 2880
Tth111I	GACNNNGTC	1 cut(s)	2265
Van91I	CCANNNNNTGG	3 cut(s)	1708, 2040, 2065
VneI	GTGCAC	1 cut(s)	1156
VpaK11BI	GGWCC	5 cut(s)	174, 341, 1208, 1568, 1856
XagI	CCTNNNNNAGG	1 cut(s)	2822
XapI	RAATTY	3 cut(s)	2256, 2714, 2907
XceI	RCATGY	3 cut(s)	1561, 2335, 2581
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2344, 2896
XhoI	CTCGAG	2 cut(s)	593, 1693
XmiI	GTMKAC	1 cut(s)	300
XmnI	GAANNNNTTC	3 cut(s)	1055, 1487, 2901
XspI	CTAG	1 cut(s)	2160
ZraI	GACGTC	2 cut(s)	858, 2656
ZrmI	AGTACT	1 cut(s)	599

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.