Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0012855

Physical Location & Seq

Genomic Coordinates Reverse (-)

20939117 .. 20943195

Gene Structure 4079 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ48339

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3093 bp

ATGCGGCTCCTCCTTCTCCTCCTCCTCCTCCACCTCCACCTCCGCCACTCTCTCTCCTCACCGCACCGTCTCCTCTCCACAGCCACAGCCACAGCCACACGCCCAATGTCCACCTACCGGGCCCTCCTCTCCCTCAAGGCCTCCATCACCGACGACCCCGACTCCCGCCTCTCCTCCTGGACCCCCAACACCAACCACTGCACCTGGGCAGGCGTCACGTGCGACTCCCGCCGTCACGTGACCTCCCTCGACCTTTCCGGCTTGAATCTCTCCGGCTATCTCTCCCCCGATATCGCCCGCCTTGTCTACCTCTCTAACCTTACTCTCGCCGACAACACGTTCTCGGGTCCTATCCCGCCCGAGATCTCGGCCCTCTCGGGGCTCCGACTCCTCAACCTCTCCAACAACGTCTTCAACCGCACGTTCCCGCCGGAGCTCTCCAACCTCACAAACCTCCGCGTGCTCGACCTCTACAACAACAACTTGACCGGAGAATTGCCGGTGTCAGTCACCCAGATGACCAACCTCAGGCACTTGCATTTGGGTGGCAACTTTTTCGAGGGTTCGATCCCGCCGGAGTACGGCCGGTTCCCGTTTCTCGAGTACTTGGCCGTCTCCGGGAACTCGATCACCGGCAAAATCCCCAAGGAGATCGGAAACCTGACCAGGCTCAAGGAGCTTTACATTGGCTACTACAACAGCTACGACGGCGGCATACCGGCCGAGATCGGGAACTTGACAGAACTCGTCCGGTTCGACGCCGCCAACTGCAATTTATCCGGCGAGATCCCGCCGGAACTGGGTCTGTTGCAAAATGTGGATACTCTGTTTCTCCAGGTGAATGCGCTTTCCGGGTCATTGACGTCTGAGCTGGGCTACCTCAAAAGCTTGAAATCCATGGACTTATCCAACAACTTGTTCTCCGGCGAGATTCCGGTCTCTTTCGCAGAGCTGAAGAACCTGACGCTTCTCAATCTCTTCAGGAACCAGCTTCACGGCGCCATTCCCGACTTCATCGGTGACTTGCCGGAGCTCCAGGTTCTGCAGCTATGGGAGAACAACTTCACTGGGTCTATTCCTCAGGGTTTGGGCAGAAATGGGAAGCTTCAAATCCTCGACTTGTCTTCGAACAAATTGACAGGAGCTCTTCCTCCTGATATGTGCACAGGAAACAATCTTCAAACCCTCATCACTCTCGGCAACTTTCTCTTCGGTCCCATTCCAGAGTCACTCGGAGACTGCCAGTCACTCAGTCGGATTCGAATGGGTGAGAACTTTCTAAATGGGTCGATTCCTAAAGGTCTATTTGGGTTGCCCAAACTCACCCAAGTGGAGCTTCAAGACAATTTCTTAGGCGGTTCATTTCCAGAGTCTGATTCAATCTCTGGTGCTCTCGGTCAGATTAGTCTCTCCAACAATCGGTTATCCGGGCCCTTGCCGCCGACCATCGGAAACTTCTCCGGCGTCCAGAAGCTTCTCCTTGACGGGAACAAGTTCTCGGGTCGAATCCCGCCGGAGATTGGACGCCTGCAGCAACTGTCAAAGATTGATTTTAGCCACAACATGTTTACGGGTCCAATTGCGCCGGCGATCAGCCAATGCAAGCTGCTGACATTTGTTGATCTCAGTCGGAATGATCTCTCCGGTGAGATTCCGAAAGAGATTACCGGTATGAGGATACTGAATTACCTCAATCTCTCGAGGAACCATCTTGTGGGGAACATCCCATCTTCCATTTCCTCAATGCAAAGCTTAACCTCGGTGGATTTTTCGTATAACAATCTTTCGGGTTTGGTTCCGGGGACTGGTCAGTTCAGCTACTTCAATTACACTTCGTTTTTGGGCAACTCTGATCTTTGTGGTCCCTATTTGGTTCCTTGCAAAGATGGAGTTGCAAATGGCACTCATCAGCCTCGCGTCAAAAGCTCATTCACTGCTTCATTGAAGCTATTGCTCGTTGTTGGGTTGCTACTCTGCTCCATCATATTTGCAGTGATTACAATAATCAAAGCTCGATCGTACAAGAGAGAGAGGGATTCCAGAGCTTGGAAGTTGACTGCATTCCAGCGCTTGGATTTCACTGTTGATGATGTCTTGGATTCACTGAAGGAAGACAACATTATAGGCAAAGGAGGTGCTGGGATTGTGTACAAAGGCGCTATGCCTAGTGGTGACAATGTGGCTGTGAAAAGACTCCCTGCAATGAGCAGAGGCTCATCTCATGATCATGGATTCAATGCTGAGATTCAGACTCTGGGTCGAATTCGACACCGTCACATTGTTAGATTGTTGGGTTTCTGTTCAAACCATGAGACAAATCTTCTGGTCTACGAGTACATGCCTAATGGGAGTTTGGGTGAGGTTCTTCATGGCAAGAAAGGAGGTCACTTGCATTGGGATACAAGGTACAAGATTGCTATTGAAGCTGCAAAGGGGCTTTGCTATCTTCACCATGACTGTTCGCCTCTGATAGTCCATAGAGATGTGAAATCGAATAACATTCTCCTCGACTCCAATTTTGAGGCCCATGTTGCTGATTTTGGCCTTGCCAAGTTCCTTCAGGATTCCGGCACGTCCGAGTGCATGTCTGCCATTGCTGGTTCTTATGGATACATCGCTCCAGAGTATGCTTACACACTGAAGGTCGATGAGAAGAGCGACGTCTATAGTTTCGGTGTAGTTCTCTTAGAACTTGTTAGTGGCAAGAAACCAGTTGGTGAATTTGGAGATGGAGTCGATATAGTCCAATGGGTTCGGAAAATGACGGATTCAAACAAGGAAGGAGTCCTGAAGATCCTTGATTCCAGACTCCCTTCTGTTCCCCTACATGAGGTTATGCACGTATTTTACGTTGCAATGCTCTGCGTTGAAGAACAAGCAGTGGAACGTCCCACCATGAGAGAAGTGGTTCAAATTCTTACAGAGCTTCCAATTCCACCAGGCTCAAAGCAAGGAGGAGACACAACAACAATCACAGACTCCCCTCAACAATCTGCCATTACTGCAATGGAATCACCAACTACCACAAAAGACACGAAAGACCATCAACAGCCACCGCCACAATCGCCACCGCCGGATCTTCTAAGCATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009908 GO:0010073 GO:0010075 GO:0016020 GO:0022414 GO:0032501 GO:0032502 GO:0033612 GO:0040008 GO:0043621 GO:0044464 GO:0048229 GO:0048367 GO:0048437 GO:0048507 GO:0048509 GO:0048608 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0061458 GO:0065007 GO:0071944 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1030

Amino Acids

112.61

Weight (kDa)

6.27

Isoelectric Point (pI)

36.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	40 - 75	2.3e-12	Leucine rich repeat N-terminal domain
LRR_14	PF23598	121 - 182	4.8e-07	Leucine-rich repeat region
LRR_4	PF12799	150 - 184	1.6e-06	Leucine Rich repeats (2 copies)
LRR_14	PF23598	176 - 282	5.5e-08	Leucine-rich repeat region
PK_Tyr_Ser-Thr	PF07714	704 - 973	1.7e-45	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	704 - 971	2.4e-44	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0012855)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49670 AT5G65700 AT5G65700
fragaria_vesca	FvH4_1g18710
malus_domestica	MD02G1194600.v1.1 MD15G1304900.v1.1
prunus_persica	Prupe.6G212200_v2.0.a1
pyrus_communis	pycom02g15850 pycom15g26730
rosa_chinensis	RchiOBHm_Chr2g0109581
rosa_laevigata	RLG00000017747
rosa_multiflora	Rmu_sc0000332.1_g000045
rosa_roxburghii	Rroxscaffold_2G00134130
rosa_rugosa	Rorug02G0160800
rosa_samantha	Rh2AG213200 Rh2BG223400 Rh2CG215200 Rh2DG218700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	866, 2664
AccB1I	GGYRCC	1 cut(s)	998
AccB7I	CCANNNNNTGG	3 cut(s)	1714, 2046, 2071
AccI	GTMKAC	2 cut(s)	306, 2328
AccII	CGCG	2 cut(s)	459, 1917
AclWI	GGATC	4 cut(s)	562, 781, 2788, 3084
AcoI	YGGCCR	3 cut(s)	583, 609, 720
AcsI	RAATTY	3 cut(s)	2262, 2720, 2913
AcuI	CTGAAG	6 cut(s)	964, 974, 2126, 2543, 2660, 2810
AcvI	CACGTG	2 cut(s)	219, 238
AcyI	GRCGYC	7 cut(s)	213, 759, 863, 999, 1464, 1525, 2661
AfaI	GTAC	6 cut(s)	581, 605, 2021, 2150, 2336, 2408
AfeI	AGCGCT	1 cut(s)	2069
AflIII	ACRYGT	2 cut(s)	336, 1563
AgeI	ACCGGT	1 cut(s)	1667
AhdI	GACNNNNNGTC	2 cut(s)	1243, 2256
AjiI	CACGTC	1 cut(s)	2574
AjnI	CCWGG	6 cut(s)	176, 203, 665, 834, 1035, 2938
AjuI	GAANNNNNNNTTGG	6 cut(s)	395, 427, 902, 934, 1320, 1352
AleI	CACNNNNGTG	1 cut(s)	1328
Alw21I	GWGCWC	6 cut(s)	438, 465, 1035, 1147, 1166, 1393
Alw26I	GTCTC	7 cut(s)	74, 619, 943, 1230, 1412, 2306, 2952
Alw44I	GTGCAC	1 cut(s)	1162
AlwI	GGATC	4 cut(s)	562, 781, 2788, 3084
AlwNI	CAGNNNCTG	4 cut(s)	1042, 1373, 1537, 2254
Ama87I	CYCGRG	6 cut(s)	343, 359, 376, 599, 1498, 1699
Aor51HI	AGCGCT	1 cut(s)	2069
AoxI	GGCC	9 cut(s)	120, 138, 369, 583, 609, 720, 1430, 2523, 2542
ApaI	GGGCCC	2 cut(s)	124, 1434
ApaLI	GTGCAC	1 cut(s)	1162
ApeKI	GCWGC	4 cut(s)	1045, 1531, 1606, 2426
ApoI	RAATTY	3 cut(s)	2262, 2720, 2913
ArsI	GACNNNNNNTTYG	2 cut(s)	2501, 2533
AsiGI	ACCGGT	1 cut(s)	1667
Asp700I	GAANNNNTTC	3 cut(s)	1061, 1493, 2907
AspLEI	GCGC	5 cut(s)	847, 1001, 1585, 2070, 2159
AsuC2I	CCSGG	5 cut(s)	119, 619, 853, 1429, 1800
AsuII	TTCGAA	2 cut(s)	1127, 1261
AvaI	CYCGRG	6 cut(s)	343, 359, 376, 599, 1498, 1699
AvaII	GGWCC	5 cut(s)	180, 347, 1214, 1574, 1862
AxyI	CCTNAGG	2 cut(s)	527, 1080
BaeGI	GKGCMC	3 cut(s)	124, 1166, 1434
BanI	GGYRCC	1 cut(s)	998
BanII	GRGCYC	6 cut(s)	124, 384, 438, 1035, 1147, 1434
BbrPI	CACGTG	2 cut(s)	219, 238
BbsI	GAAGAC	3 cut(s)	403, 1116, 2118
Bbv12I	GWGCWC	6 cut(s)	438, 465, 1035, 1147, 1166, 1393
BbvI	GCAGC	4 cut(s)	1057, 1543, 1593, 2413
BccI	CCATC	8 cut(s)	152, 1454, 1716, 1735, 1880, 1988, 2723, 3051
BceAI	ACGGC	5 cut(s)	216, 596, 598, 724, 1012
BcgI	CGANNNNNNTGC	2 cut(s)	538, 572
BciT130I	CCWGG	6 cut(s)	178, 205, 667, 836, 1037, 2940
BciVI	GTATCC	4 cut(s)	814, 1671, 2392, 2603
BclI	TGATCA	1 cut(s)	2224
BcnI	CCSGG	5 cut(s)	119, 619, 853, 1429, 1800
BcoDI	GTCTC	7 cut(s)	74, 619, 943, 1230, 1412, 2306, 2952
BfaI	CTAG	1 cut(s)	2166
BfmI	CTRYAG	3 cut(s)	1043, 1529, 2665
BfoI	RGCGCY	3 cut(s)	1002, 2071, 2160
BfuI	GTATCC	4 cut(s)	814, 1671, 2392, 2603
BglII	AGATCT	1 cut(s)	363
BisI	GCNGC	8 cut(s)	5, 712, 762, 1046, 1439, 1532, 1607, 2427
BlsI	GCNGC	8 cut(s)	6, 713, 763, 1047, 1440, 1533, 1608, 2428
BmcAI	AGTACT	1 cut(s)	605
Bme18I	GGWCC	5 cut(s)	180, 347, 1214, 1574, 1862
BmeRI	GACNNNNNGTC	2 cut(s)	1243, 2256
BmeT110I	CYCGRG	6 cut(s)	343, 359, 376, 599, 1498, 1699
BmgBI	CACGTC	1 cut(s)	2574
BmrI	ACTGGG	2 cut(s)	809, 1077
BmuI	ACTGGG	2 cut(s)	809, 1077
BpiI	GAAGAC	3 cut(s)	403, 1116, 2118
BpmI	CTGGAG	3 cut(s)	818, 1019, 2604
Bpu14I	TTCGAA	2 cut(s)	1127, 1261
BpuEI	CTTGAG	2 cut(s)	119, 656
BpuMI	CCSGG	5 cut(s)	119, 619, 853, 1429, 1800
BsaAI	YACGTR	3 cut(s)	219, 238, 2842
BsaHI	GRCGYC	7 cut(s)	213, 759, 863, 999, 1464, 1525, 2661
BsaI	GGTCTC	1 cut(s)	943
BsaJI	CCNNGG	5 cut(s)	204, 645, 897, 1758, 1799
BsaWI	WCCGGW	5 cut(s)	488, 750, 934, 1643, 1667
Bse118I	RCCGGY	6 cut(s)	499, 585, 632, 718, 1585, 1667
Bse1I	ACTGG	5 cut(s)	804, 1072, 1243, 1810, 2711
Bse21I	CCTNAGG	2 cut(s)	527, 1080
Bse3DI	GCAATG	4 cut(s)	2208, 2592, 2862, 3012
BseBI	CCWGG	6 cut(s)	178, 205, 667, 836, 1037, 2940
BseDI	CCNNGG	5 cut(s)	204, 645, 897, 1758, 1799
BseGI	GGATG	1 cut(s)	1722
BseMI	GCAATG	4 cut(s)	2208, 2592, 2862, 3012
BseMII	CTCAG	6 cut(s)	541, 858, 1094, 1264, 1639, 2232
BseNI	ACTGG	5 cut(s)	804, 1072, 1243, 1810, 2711
BseSI	GKGCMC	3 cut(s)	124, 1166, 1434
BseX3I	CGGCCG	2 cut(s)	583, 720
BseXI	GCAGC	4 cut(s)	1057, 1543, 1593, 2413
BseYI	CCCAGC	2 cut(s)	871, 2138
BsgI	GTGCAG	1 cut(s)	184
Bsh1236I	CGCG	2 cut(s)	459, 1917
Bsh1285I	CGRYCG	3 cut(s)	586, 723, 2018
BshFI	GGCC	9 cut(s)	122, 140, 371, 585, 611, 722, 1432, 2525, 2544
BshNI	GGYRCC	1 cut(s)	998
BshTI	ACCGGT	1 cut(s)	1667
BsiEI	CGRYCG	3 cut(s)	586, 723, 2018
BsiHKAI	GWGCWC	6 cut(s)	438, 465, 1035, 1147, 1166, 1393
BsiHKCI	CYCGRG	6 cut(s)	343, 359, 376, 599, 1498, 1699
BslFI	GGGAC	4 cut(s)	1200, 1816, 1848, 2874
BsmAI	GTCTC	7 cut(s)	74, 619, 943, 1230, 1412, 2306, 2952
BsmBI	CGTCTC	2 cut(s)	74, 619
BsmFI	GGGAC	4 cut(s)	1200, 1816, 1848, 2874
BsmI	GAATGC	2 cut(s)	847, 2060
BsnI	GGCC	9 cut(s)	122, 140, 371, 585, 611, 722, 1432, 2525, 2544
Bso31I	GGTCTC	1 cut(s)	943
BsoBI	CYCGRG	6 cut(s)	343, 359, 376, 599, 1498, 1699
Bsp119I	TTCGAA	2 cut(s)	1127, 1261
Bsp120I	GGGCCC	2 cut(s)	120, 1430
Bsp1286I	GDGCHC	9 cut(s)	124, 384, 438, 465, 1035, 1147, 1166, 1393, 1434
Bsp1407I	TGTACA	1 cut(s)	2148
Bsp19I	CCATGG	1 cut(s)	897
BspANI	GGCC	9 cut(s)	122, 140, 371, 585, 611, 722, 1432, 2525, 2544
BspCNI	CTCAG	6 cut(s)	540, 859, 1093, 1263, 1638, 2233
BspFNI	CGCG	2 cut(s)	459, 1917
BspHI	TCATGA	1 cut(s)	2221
BspMAI	CTGCAG	2 cut(s)	1047, 1533
BspPI	GGATC	4 cut(s)	562, 781, 2788, 3084
BspQI	GCTCTTC	2 cut(s)	1152, 2648
BspT104I	TTCGAA	2 cut(s)	1127, 1261
BspT107I	GGYRCC	1 cut(s)	998
BspTNI	GGTCTC	1 cut(s)	943
BsrDI	GCAATG	4 cut(s)	2208, 2592, 2862, 3012
BsrFI	RCCGGY	6 cut(s)	499, 585, 632, 718, 1585, 1667
BsrGI	TGTACA	1 cut(s)	2148
BsrI	ACTGG	5 cut(s)	804, 1072, 1243, 1810, 2711
BssAI	RCCGGY	6 cut(s)	499, 585, 632, 718, 1585, 1667
BssECI	CCNNGG	5 cut(s)	204, 645, 897, 1758, 1799
BssNI	GRCGYC	7 cut(s)	213, 759, 863, 999, 1464, 1525, 2661
BssT1I	CCWWGG	2 cut(s)	645, 897
Bst2UI	CCWGG	6 cut(s)	178, 205, 667, 836, 1037, 2940
Bst4CI	ACNGT	5 cut(s)	68, 1539, 2083, 2273, 2459
Bst6I	CTCTTC	4 cut(s)	983, 1152, 1214, 2648
BstACI	GRCGYC	7 cut(s)	213, 759, 863, 999, 1464, 1525, 2661
BstAUI	TGTACA	1 cut(s)	2148
BstBAI	YACGTR	3 cut(s)	219, 238, 2842
BstBI	TTCGAA	2 cut(s)	1127, 1261
BstC8I	GCNNGC	6 cut(s)	211, 298, 461, 1529, 1587, 1604
BstDEI	CTNAG	9 cut(s)	527, 867, 1080, 1250, 1351, 1625, 2241, 2686, 3083
BstDSI	CCRYGG	1 cut(s)	897
BstENI	CCTNNNNNAGG	1 cut(s)	2828
BstF5I	GGATG	1 cut(s)	1722
BstFNI	CGCG	2 cut(s)	459, 1917
BstH2I	RGCGCY	3 cut(s)	1002, 2071, 2160
BstHHI	GCGC	5 cut(s)	847, 1001, 1585, 2070, 2159
BstMAI	GTCTC	7 cut(s)	74, 619, 943, 1230, 1412, 2306, 2952
BstMCI	CGRYCG	3 cut(s)	586, 723, 2018
BstNI	CCWGG	6 cut(s)	178, 205, 667, 836, 1037, 2940
BstNSI	RCATGY	3 cut(s)	1567, 2341, 2587
BstSFI	CTRYAG	3 cut(s)	1043, 1529, 2665
BstSLI	GKGCMC	3 cut(s)	124, 1166, 1434
BstUI	CGCG	2 cut(s)	459, 1917
BstV1I	GCAGC	4 cut(s)	1057, 1543, 1593, 2413
BstV2I	GAAGAC	3 cut(s)	403, 1116, 2118
BstX2I	RGATCY	4 cut(s)	363, 786, 2793, 3076
BstYI	RGATCY	4 cut(s)	363, 786, 2793, 3076
BstZI	CGGCCG	2 cut(s)	583, 720
Bsu36I	CCTNAGG	2 cut(s)	527, 1080
BsuI	GTATCC	4 cut(s)	814, 1671, 2392, 2603
BsuRI	GGCC	9 cut(s)	122, 140, 371, 585, 611, 722, 1432, 2525, 2544
BtgI	CCRYGG	1 cut(s)	897
BtgZI	GCGATG	1 cut(s)	2599
BtrI	CACGTC	1 cut(s)	2574
BtsCI	GGATG	1 cut(s)	1722
BtsI	GCAGTG	4 cut(s)	196, 1932, 1998, 2886
BtsIMutI	CAGTG	8 cut(s)	196, 1065, 1932, 1998, 2079, 2102, 2636, 2886
Cac8I	GCNNGC	6 cut(s)	211, 298, 461, 1529, 1587, 1604
CaiI	CAGNNNCTG	4 cut(s)	1042, 1373, 1537, 2254
CciI	TCATGA	1 cut(s)	2221
CfoI	GCGC	5 cut(s)	847, 1001, 1585, 2070, 2159
Cfr10I	RCCGGY	6 cut(s)	499, 585, 632, 718, 1585, 1667
CseI	GACGC	6 cut(s)	202, 767, 973, 1453, 1533, 1906
Csp6I	GTAC	6 cut(s)	580, 604, 2020, 2149, 2335, 2407
CspAI	ACCGGT	1 cut(s)	1667
CviQI	GTAC	6 cut(s)	580, 604, 2020, 2149, 2335, 2407
DdeI	CTNAG	9 cut(s)	527, 867, 1080, 1250, 1351, 1625, 2241, 2686, 3083
DinI	GGCGCC	1 cut(s)	1000
DriI	GACNNNNNGTC	2 cut(s)	1243, 2256
EaeI	YGGCCR	3 cut(s)	583, 609, 720
EagI	CGGCCG	2 cut(s)	583, 720
Eam1104I	CTCTTC	4 cut(s)	983, 1152, 1214, 2648
Eam1105I	GACNNNNNGTC	2 cut(s)	1243, 2256
EarI	CTCTTC	4 cut(s)	983, 1152, 1214, 2648
EciI	GGCGGA	1 cut(s)	32
Ecl136II	GAGCTC	3 cut(s)	436, 1033, 1145
EclXI	CGGCCG	2 cut(s)	583, 720
Eco130I	CCWWGG	2 cut(s)	645, 897
Eco147I	AGGCCT	1 cut(s)	140
Eco24I	GRGCYC	6 cut(s)	124, 384, 438, 1035, 1147, 1434
Eco31I	GGTCTC	1 cut(s)	943
Eco32I	GATATC	1 cut(s)	292
Eco47I	GGWCC	5 cut(s)	180, 347, 1214, 1574, 1862
Eco47III	AGCGCT	1 cut(s)	2069
Eco52I	CGGCCG	2 cut(s)	583, 720
Eco53kI	GAGCTC	3 cut(s)	436, 1033, 1145
Eco57I	CTGAAG	6 cut(s)	964, 974, 2126, 2543, 2660, 2810
Eco72I	CACGTG	2 cut(s)	219, 238
Eco81I	CCTNAGG	2 cut(s)	527, 1080
Eco88I	CYCGRG	6 cut(s)	343, 359, 376, 599, 1498, 1699
EcoICRI	GAGCTC	3 cut(s)	436, 1033, 1145
EcoNI	CCTNNNNNAGG	1 cut(s)	2828
EcoO109I	RGGNCCY	3 cut(s)	121, 347, 1431
EcoRI	GAATTC	1 cut(s)	2262
EcoRII	CCWGG	6 cut(s)	176, 203, 665, 834, 1035, 2938
EcoRV	GATATC	1 cut(s)	292
EcoT14I	CCWWGG	2 cut(s)	645, 897
EcoT38I	GRGCYC	6 cut(s)	124, 384, 438, 1035, 1147, 1434
EgeI	GGCGCC	1 cut(s)	1000
EheI	GGCGCC	1 cut(s)	1000
ErhI	CCWWGG	2 cut(s)	645, 897
Esp3I	CGTCTC	2 cut(s)	74, 619
FalI	AAGNNNNNCTT	2 cut(s)	1320, 1352
FaqI	GGGAC	4 cut(s)	1200, 1816, 1848, 2874
FauI	CCCGC	8 cut(s)	173, 236, 305, 363, 435, 579, 798, 1518
FbaI	TGATCA	1 cut(s)	2224
FblI	GTMKAC	2 cut(s)	306, 2328
Fnu4HI	GCNGC	8 cut(s)	5, 712, 762, 1046, 1439, 1532, 1607, 2427
FokI	GGATG	1 cut(s)	1709
FriOI	GRGCYC	6 cut(s)	124, 384, 438, 1035, 1147, 1434
Fsp4HI	GCNGC	8 cut(s)	5, 712, 762, 1046, 1439, 1532, 1607, 2427
FspBI	CTAG	1 cut(s)	2166
GlaI	GCGC	5 cut(s)	846, 1000, 1584, 2069, 2158
GluI	GCNGC	8 cut(s)	5, 712, 762, 1046, 1439, 1532, 1607, 2427
GsaI	CCCAGC	2 cut(s)	875, 2142
GsuI	CTGGAG	3 cut(s)	818, 1019, 2604
HaeII	RGCGCY	3 cut(s)	1002, 2071, 2160
HaeIII	GGCC	9 cut(s)	122, 140, 371, 585, 611, 722, 1432, 2525, 2544
HgaI	GACGC	6 cut(s)	202, 767, 973, 1453, 1533, 1906
HhaI	GCGC	5 cut(s)	847, 1001, 1585, 2070, 2159
Hin1I	GRCGYC	7 cut(s)	213, 759, 863, 999, 1464, 1525, 2661
Hin6I	GCGC	5 cut(s)	845, 999, 1583, 2068, 2157
HinP1I	GCGC	5 cut(s)	845, 999, 1583, 2068, 2157
HincII	GTYRAC	1 cut(s)	2055
HindII	GTYRAC	1 cut(s)	2055
HindIII	AAGCTT	4 cut(s)	886, 1103, 1472, 1750
Hpy166II	GTNNAC	7 cut(s)	111, 307, 1164, 1569, 2055, 2149, 2329
Hpy8I	GTNNAC	7 cut(s)	111, 307, 1164, 1569, 2055, 2149, 2329
Hpy99I	CGWCG	4 cut(s)	155, 710, 761, 2663
HpyAV	CCTTC	6 cut(s)	23, 2101, 2567, 2635, 2774, 2823
HpyCH4III	ACNGT	5 cut(s)	68, 1539, 2083, 2273, 2459
HpyF3I	CTNAG	9 cut(s)	527, 867, 1080, 1250, 1351, 1625, 2241, 2686, 3083
Hsp92I	GRCGYC	7 cut(s)	213, 759, 863, 999, 1464, 1525, 2661
HspAI	GCGC	5 cut(s)	845, 999, 1583, 2068, 2157
KasI	GGCGCC	1 cut(s)	998
KroI	GCCGGC	1 cut(s)	1585
KroNI	GCCGGC	1 cut(s)	1587
Ksp22I	TGATCA	1 cut(s)	2224
LguI	GCTCTTC	2 cut(s)	1152, 2648
Lsp1109I	GCAGC	4 cut(s)	1057, 1543, 1593, 2413
MaeI	CTAG	1 cut(s)	2166
MfeI	CAATTG	1 cut(s)	1578
MflI	RGATCY	4 cut(s)	363, 786, 2793, 3076
MhlI	GDGCHC	9 cut(s)	124, 384, 438, 465, 1035, 1147, 1166, 1393, 1434
Mly113I	GGCGCC	1 cut(s)	999
MmeI	TCCRAC	7 cut(s)	409, 426, 465, 933, 1235, 1437, 1610
MreI	CGCCGGCG	1 cut(s)	1585
MroNI	GCCGGC	1 cut(s)	1585
MroXI	GAANNNNTTC	3 cut(s)	1061, 1493, 2907
MseI	TTAA	1 cut(s)	1754
MslI	CAYNNNNRTG	5 cut(s)	515, 543, 1328, 2226, 2481
MunI	CAATTG	1 cut(s)	1578
Mva1269I	GAATGC	2 cut(s)	847, 2060
MvaI	CCWGG	6 cut(s)	178, 205, 667, 836, 1037, 2940
MvnI	CGCG	2 cut(s)	459, 1917
NaeI	GCCGGC	1 cut(s)	1587
NarI	GGCGCC	1 cut(s)	999
NciI	CCSGG	5 cut(s)	119, 619, 853, 1429, 1800
NcoI	CCATGG	1 cut(s)	897
NgoMIV	GCCGGC	1 cut(s)	1585
NmeAIII	GCCGAG	3 cut(s)	347, 748, 1176
NspI	RCATGY	3 cut(s)	1567, 2341, 2587
NspV	TTCGAA	2 cut(s)	1127, 1261
OliI	CACNNNNGTG	1 cut(s)	1328
PaeR7I	CTCGAG	2 cut(s)	599, 1699
PagI	TCATGA	1 cut(s)	2221
PceI	AGGCCT	1 cut(s)	140
PciI	ACATGT	1 cut(s)	1563
PciSI	GCTCTTC	2 cut(s)	1152, 2648
PcsI	WCGNNNNNNNCGW	2 cut(s)	753, 2847
PctI	GAATGC	2 cut(s)	847, 2060
PdiI	GCCGGC	1 cut(s)	1587
PdmI	GAANNNNTTC	3 cut(s)	1061, 1493, 2907
PflFI	GACNNNGTC	1 cut(s)	2271
PflMI	CCANNNNNTGG	3 cut(s)	1714, 2046, 2071
PfoI	TCCNGGA	2 cut(s)	176, 617
PinAI	ACCGGT	1 cut(s)	1667
PkrI	GCNGC	8 cut(s)	6, 713, 763, 1047, 1440, 1533, 1608, 2428
Ple19I	CGATCG	1 cut(s)	2018
PluTI	GGCGCC	1 cut(s)	1002
PmaCI	CACGTG	2 cut(s)	219, 238
PmlI	CACGTG	2 cut(s)	219, 238
Ppu21I	YACGTR	3 cut(s)	219, 238, 2842
PpuMI	RGGWCCY	1 cut(s)	347
PscI	ACATGT	1 cut(s)	1563
Psp124BI	GAGCTC	3 cut(s)	438, 1035, 1147
Psp5II	RGGWCCY	1 cut(s)	347
Psp6I	CCWGG	6 cut(s)	176, 203, 665, 834, 1035, 2938
PspCI	CACGTG	2 cut(s)	219, 238
PspFI	CCCAGC	2 cut(s)	871, 2138
PspGI	CCWGG	6 cut(s)	176, 203, 665, 834, 1035, 2938
PspOMI	GGGCCC	2 cut(s)	120, 1430
PspPPI	RGGWCCY	1 cut(s)	347
PsrI	GAACNNNNNNTAC	2 cut(s)	572, 604
PstI	CTGCAG	2 cut(s)	1047, 1533
PstNI	CAGNNNCTG	4 cut(s)	1042, 1373, 1537, 2254
PsuI	RGATCY	4 cut(s)	363, 786, 2793, 3076
PsyI	GACNNNGTC	1 cut(s)	2271
PvuI	CGATCG	1 cut(s)	2018
RsaI	GTAC	6 cut(s)	581, 605, 2021, 2150, 2336, 2408
RsaNI	GTAC	6 cut(s)	580, 604, 2020, 2149, 2335, 2407
RseI	CAYNNNNRTG	5 cut(s)	515, 543, 1328, 2226, 2481
SacI	GAGCTC	3 cut(s)	438, 1035, 1147
SapI	GCTCTTC	2 cut(s)	1152, 2648
SaqAI	TTAA	1 cut(s)	1754
SatI	GCNGC	8 cut(s)	5, 712, 762, 1046, 1439, 1532, 1607, 2427
ScaI	AGTACT	1 cut(s)	605
SduI	GDGCHC	9 cut(s)	124, 384, 438, 465, 1035, 1147, 1166, 1393, 1434
SfcI	CTRYAG	3 cut(s)	1043, 1529, 2665
SfoI	GGCGCC	1 cut(s)	1000
Sfr274I	CTCGAG	2 cut(s)	599, 1699
SfuI	TTCGAA	2 cut(s)	1127, 1261
SgrAI	CRCCGGYG	1 cut(s)	1585
SinI	GGWCC	5 cut(s)	180, 347, 1214, 1574, 1862
SlaI	CTCGAG	2 cut(s)	599, 1699
SmiMI	CAYNNNNRTG	5 cut(s)	515, 543, 1328, 2226, 2481
SmlI	CTYRAG	4 cut(s)	134, 599, 671, 1699
SmoI	CTYRAG	4 cut(s)	134, 599, 671, 1699
SseBI	AGGCCT	1 cut(s)	140
SspDI	GGCGCC	1 cut(s)	998
SspMI	CTAG	1 cut(s)	2166
SstI	GAGCTC	3 cut(s)	438, 1035, 1147
StuI	AGGCCT	1 cut(s)	140
StyI	CCWWGG	2 cut(s)	645, 897
TaaI	ACNGT	5 cut(s)	68, 1539, 2083, 2273, 2459
TaqII	GACCGA	2 cut(s)	1202, 1385
TatI	WGTACW	3 cut(s)	603, 2148, 2334
TauI	GCSGC	4 cut(s)	7, 714, 764, 1441
Tru1I	TTAA	1 cut(s)	1754
Tru9I	TTAA	1 cut(s)	1754
TscAI	CASTG	8 cut(s)	203, 1072, 1939, 1998, 2086, 2109, 2643, 2886
TseI	GCWGC	4 cut(s)	1045, 1531, 1606, 2426
TspDTI	ATGAA	4 cut(s)	1003, 1350, 1929, 2357
TspGWI	ACGGA	1 cut(s)	2780
TspRI	CASTG	8 cut(s)	203, 1072, 1939, 1998, 2086, 2109, 2643, 2886
Tth111I	GACNNNGTC	1 cut(s)	2271
Van91I	CCANNNNNTGG	3 cut(s)	1714, 2046, 2071
VneI	GTGCAC	1 cut(s)	1162
VpaK11BI	GGWCC	5 cut(s)	180, 347, 1214, 1574, 1862
XagI	CCTNNNNNAGG	1 cut(s)	2828
XapI	RAATTY	3 cut(s)	2262, 2720, 2913
XceI	RCATGY	3 cut(s)	1567, 2341, 2587
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2902, 3004
XhoI	CTCGAG	2 cut(s)	599, 1699
XmiI	GTMKAC	2 cut(s)	306, 2328
XmnI	GAANNNNTTC	3 cut(s)	1061, 1493, 2907
XspI	CTAG	1 cut(s)	2166
ZraI	GACGTC	2 cut(s)	864, 2662
ZrmI	AGTACT	1 cut(s)	605

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.