Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0012855

Physical Location & Seq

Genomic Coordinates Forward (+)

14386333 .. 14391505

Gene Structure 5173 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0160800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2778 bp

ATGCCGCCGGTGGCGGAAGAAGCGGAGGAGGGGGCGCAGTACTCGTTTGCTATGGAGTATAAAGGTCCTCCGGTGAGTTACGATCTTCCGAAGGCGGTTCCGATCAACGTCGACAGAATCCCCGTGGCGGCTGTCGTCGGACAGGTGTCTATTCCGGATAAGTTGTCGTTGCCGGTGGTCCAGCCGGTTCTGGCGCCCGGTTCGCTGATGAAAACGTTCTCCAAAGAGCTCAAGGCTTCCCAGTCGACGGTGTCTCCGACCTCGGTGATCGCTTTCGATCGGAGAAGCGAGGACGACACGACGAAGGAGTTGGAAGGGTCGGAATCGGCGACGGTTTCGCCGGCGTCGGTGATTGGGTTCGAGGAGAGAGCTGAGGAGAGGGGGGCTGGGTGTGGTGCTTTGTCCGGAGAATTGAGTAGCTCCGGCGGGTTGGAGTTTTCGAACGGGTTGAATTACGGGTCGGGTGAGCTGTCCGATGTGAATAATGGTAGCTGGGCTTGGAGCGGAGGAGCATTGACGGCTCCGAGTGGTGGGAAGGAGAGTTTGGATTTGAATCAACCCGAACCCGATTGGGCTTCCTCCGAGTCCGTTTTGAGCTTGGACTATCCGTCTTCTCGGGTTTCCTCCACCAAGGCTGTGGACTGCGATGTGAAACGACCCGCTGTGGTGACGTTTCGGGACATTGAATCTGATGATGATGATGGTGTTGAGGAAGATGAGGCTGAGGTTGTGCCGGTGAAGCGGGAGCCTCAGAGCAAAGGAAAGAAGAAGTCTTGTTATAGATGTCTGAAGGGGACTAGGTTCACTGAGAAGGAGGTGTGCATTGTTTGTGACGCCAAGTACTGCAGTAGTTGTGTTCTGAGAGCTATGGGGTCGATGCCGGAAGGCCGAAAATGTGTTACTTGTATTGGATTTCCTATTGATGAGAACAAAAGAGGGTGCTTGGGGAAGTGCTCTAGGATGCTCAAGCGGCTGCTGAATGATTTGGAGGTTCGGCAGGTGATGAAAGCCGAAAAGTTTTGTGAAGCAAATCAGCTACCGCCGAATTACATTTGTGTGAATGGACAGCCTCTTTGCCACGATGAGCTTGTTGTGTTACAGACCTGCTCAAACCCCCCGAAGAAGCTAAAACCAGGAAACTATTGGTATGATAAGGTTTCTGGACTTTGGGGAAAGGAAGGACAGAAGCCTTTGAAGGTAATCAGTCCCCATCTTAGTGTTGGGGGTCCCATCAAGGCAAATGCTAGTAATGGGAACACACAGGTTTTTATCAATGGCCGGGAAATTACCAAAGTTGAGCTTCGGATGTTACAGTTGGCAGGAGTTCAGTGTGCTGGTAACCCACACTTTTGGGTGAATGAGGATGGGTCATACCAAGAGGAGGGCCAGAAAAATACTAAAGGATACATATGGGGAAAGGCTGGAACCAAGCTTGTTTGTGCTGTCTTGTCACTGCCAGTGCCTTCTAAATCTTCGAATCCTTGTGGAGAACCCCTGAGCTATGTTGGTAGCAGAGTCGTCCCTGATTACATTGAGCAGAGAATACTTCAGAAAATTCTTTTAGTTGGATATAATGGATCCGGAACAAGTACTATATTCAAACAGGCCAAGATTCTATATAAAGCTATCCCTTTCTCGGAAGATGAGCGTGAAAATATCAAGTTTACAATACAGAGCAGTGTATATGGCTATCTTGGTATACTACTGGAAGGACGTGAGCGTTTTGAAGAGGAAAGTTTGGCTGAGATCAGAAATCAATGTTCTTCTAGTCAAACAGATGCGAGAGGGAACAACAACAAAACTCTTTACTCCATTGGCCCGAGACTGAAAGCATTTTCTGATTGGCTTCTCAAGATAATGGTGTCAGGTGATTTGGAAGCCATTTTCCCAGCTGCTACCCGTGAATATGCACCATTGGTTGAGGAGTTGTGGAATGATTCAGCCATTCAAGCTACATTCAAGCGGAGAAGTGAATTGGAAATGTTACCTGGTGTTGCTACTTATTTCATAGAGAGGGCTGTTGACATATTAAGAGTAGACTATGAGCCTTCGGATCTGGATATTCTATATGCTGAAGGTGTTACTTCATCTAATGGCCTTGCTTGTGTAGACTTCTCATTTCCACCGTCGGCATCTGAAGATAACATCAATCATAATAATGATCAGCAGGACTCTTTGCTTAGGTACCAACTGATTAGAGTAAATGCAAGAGGACTTGGAGAAAACTGCAAATGGTTGGAGATGTTTGAAGATGTTGGAATGGTCATATTCTGTGTATCCTTGAGCGACTATGATCAGTTTTCTGTGGATGGTAGTGGGTCCTTTTCAAACAAGATGCTACAGACCAGAAGTTTCTTTGAAAGCATCATTACTCATCCAACATTTGAACATATGGACTTCCTTTTGATTCTAAACAAGTTTGATGTGCTTGAGGAAAAGGTAGAAAGGGTACCCCTTACTCAGTGTGACTGGTTTGATGATTTCCATCCAGTGGTTAGCCGTCATCGGTCCAGCAGCAACAACAGTAGCAACAACATTAATAGTAGCCCCTCGCTGGGTCATCTAGCAGCTCACTACATTGCAGTAAAGTTCAAGAGGCTATATTCTTCTCTAACTGGAAAGAAACTGTACGTTTCTCTGGTAAAAGGGTTGCAGCCTAATAGCGTAGATGCAGCTCTTAAATATTCAAGGGAGATTTTAAAATGGGATGAAGAGAGAGGCAACTTCAGCTTTGATTACTCTGTCTATAGCACTGAGGCAAGCTCTTATTCACATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009908 GO:0010073 GO:0010075 GO:0016020 GO:0022414 GO:0032501 GO:0032502 GO:0033612 GO:0040008 GO:0043621 GO:0044464 GO:0048229 GO:0048367 GO:0048437 GO:0048507 GO:0048509 GO:0048608 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0061458 GO:0065007 GO:0071944 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

925

Amino Acids

102.13

Weight (kDa)

5.22

Isoelectric Point (pI)

47.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
G-alpha	PF00503	517 - 898	9.1e-63	G-protein alpha subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012855)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49670 AT5G65700 AT5G65700
fragaria_vesca	FvH4_1g18710
malus_domestica	MD02G1194600.v1.1 MD15G1304900.v1.1
prunus_persica	Prupe.6G212200_v2.0.a1
pyrus_communis	pycom02g15850 pycom15g26730
rosa_chinensis	RchiOBHm_Chr2g0109581
rosa_laevigata	RLG00000017747
rosa_multiflora	Rmu_sc0000332.1_g000045
rosa_roxburghii	Rroxscaffold_2G00134130
rosa_rugosa	Rorug02G0160800
rosa_samantha	Rh2AG213200 Rh2BG223400 Rh2CG215200 Rh2DG218700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	988
Acc36I	ACCTGC	2 cut(s)	988, 1112
Acc65I	GGTACC	2 cut(s)	2184, 2449
AccB1I	GGYRCC	3 cut(s)	193, 2184, 2449
AccB7I	CCANNNNNTGG	1 cut(s)	2491
AccBSI	CCGCTC	1 cut(s)	504
AccI	GTMKAC	5 cut(s)	111, 245, 1699, 2037, 2109
AccIII	TCCGGA	3 cut(s)	154, 404, 1580
AclI	AACGTT	1 cut(s)	215
AclWI	GGATC	3 cut(s)	1572, 1585, 2061
AcoI	YGGCCR	1 cut(s)	1276
AcsI	RAATTY	1 cut(s)	1554
AcuI	CTGAAG	5 cut(s)	809, 1532, 2094, 2157, 2710
AcyI	GRCGYC	3 cut(s)	194, 344, 834
AfaI	GTAC	6 cut(s)	41, 842, 1591, 2186, 2451, 2630
AfiI	CCNNNNNNNGG	8 cut(s)	127, 247, 530, 1381, 1636, 2491, 2554, 2555
AhdI	GACNNNNNGTC	1 cut(s)	607
AjiI	CACGTC	1 cut(s)	1715
AjnI	CCWGG	2 cut(s)	1132, 1987
AjuI	GAANNNNNNNTTGG	2 cut(s)	527, 559
Alw21I	GWGCWC	2 cut(s)	231, 956
Alw26I	GTCTC	2 cut(s)	258, 1816
AlwI	GGATC	3 cut(s)	1572, 1585, 2061
Ama87I	CYCGRG	2 cut(s)	615, 1819
Aor13HI	TCCGGA	3 cut(s)	154, 404, 1580
AoxI	GGCC	6 cut(s)	886, 1276, 1384, 1605, 1816, 2095
ApeKI	GCWGC	6 cut(s)	973, 1892, 2514, 2567, 2653, 2672
ApoI	RAATTY	1 cut(s)	1554
ArsI	GACNNNNNNTTYG	2 cut(s)	1056, 1088
AseI	ATTAAT	1 cut(s)	2538
Asp700I	GAANNNNTTC	1 cut(s)	215
Asp718I	GGTACC	2 cut(s)	2184, 2449
AspLEI	GCGC	2 cut(s)	37, 196
AspS9I	GGNCC	7 cut(s)	65, 178, 1226, 1384, 1817, 2319, 2508
AsuC2I	CCSGG	2 cut(s)	198, 1280
AsuHPI	GGTGA	9 cut(s)	85, 277, 361, 476, 679, 748, 1012, 1366, 1880
AsuII	TTCGAA	2 cut(s)	440, 1475
AvaI	CYCGRG	2 cut(s)	615, 1819
AvaII	GGWCC	5 cut(s)	65, 178, 1226, 2319, 2508
BamHI	GGATCC	1 cut(s)	1577
BanI	GGYRCC	3 cut(s)	193, 2184, 2449
BanII	GRGCYC	1 cut(s)	231
BbsI	GAAGAC	1 cut(s)	603
Bbv12I	GWGCWC	2 cut(s)	231, 956
BbvCI	CCTCAGC	2 cut(s)	372, 723
BbvI	GCAGC	6 cut(s)	960, 1879, 2526, 2579, 2665, 2684
BccI	CCATC	6 cut(s)	695, 1218, 1238, 1358, 2303, 2493
BceAI	ACGGC	2 cut(s)	534, 2484
BciT130I	CCWGG	2 cut(s)	1134, 1989
BciVI	GTATCC	2 cut(s)	1397, 2287
BclI	TGATCA	2 cut(s)	2161, 2293
BcnI	CCSGG	2 cut(s)	198, 1280
BcoDI	GTCTC	2 cut(s)	258, 1816
BfaI	CTAG	5 cut(s)	798, 957, 1245, 1767, 2564
BfmI	CTRYAG	3 cut(s)	844, 2339, 2746
BfoI	RGCGCY	1 cut(s)	197
BfuAI	ACCTGC	2 cut(s)	988, 1112
BfuI	GTATCC	2 cut(s)	1397, 2287
BisI	GCNGC	9 cut(s)	5, 129, 971, 974, 1893, 2515, 2568, 2654, 2673
BlsI	GCNGC	9 cut(s)	6, 130, 972, 975, 1894, 2516, 2569, 2655, 2674
BmcAI	AGTACT	3 cut(s)	41, 842, 1591
Bme1390I	CCNGG	4 cut(s)	198, 1134, 1280, 1989
Bme18I	GGWCC	5 cut(s)	65, 178, 1226, 2319, 2508
BmeRI	GACNNNNNGTC	1 cut(s)	607
BmeT110I	CYCGRG	2 cut(s)	615, 1819
BmgBI	CACGTC	1 cut(s)	1715
BmgT120I	GGNCC	7 cut(s)	65, 178, 1226, 1384, 1817, 2319, 2508
BmrFI	CCNGG	4 cut(s)	198, 1134, 1280, 1989
BmrI	ACTGGG	1 cut(s)	235
BmsI	GCATC	7 cut(s)	867, 951, 1768, 2141, 2325, 2373, 2659
BmuI	ACTGGG	1 cut(s)	235
BoxI	GACNNNNGTC	1 cut(s)	145
BpiI	GAAGAC	1 cut(s)	603
BplI	GAGNNNNNCTC	1 cut(s)	2747
Bpu10I	CCTNAGC	4 cut(s)	372, 723, 1496, 2180
Bpu14I	TTCGAA	2 cut(s)	440, 1475
BpuEI	CTTGAG	5 cut(s)	215, 950, 1835, 2302, 2450
BpuMI	CCSGG	2 cut(s)	198, 1280
BsaBI	GATNNNNATC	2 cut(s)	552, 2484
BsaHI	GRCGYC	3 cut(s)	194, 344, 834
BsaJI	CCNNGG	3 cut(s)	123, 261, 630
BsaWI	WCCGGW	4 cut(s)	70, 154, 404, 1580
BsaXI	ACNNNNNCTCC	2 cut(s)	238, 268
Bsc4I	CCNNNNNNNGG	8 cut(s)	127, 247, 530, 1381, 1636, 2491, 2554, 2555
Bse118I	RCCGGY	5 cut(s)	7, 172, 184, 340, 733
Bse1I	ACTGG	6 cut(s)	241, 1457, 1710, 2474, 2489, 2620
Bse3DI	GCAATG	1 cut(s)	2577
Bse8I	GATNNNNATC	2 cut(s)	552, 2484
BseAI	TCCGGA	3 cut(s)	154, 404, 1580
BseBI	CCWGG	2 cut(s)	1134, 1989
BseDI	CCNNGG	3 cut(s)	123, 261, 630
BseGI	GGATG	7 cut(s)	966, 1311, 1369, 2314, 2374, 2485, 2713
BseJI	GATNNNNATC	2 cut(s)	552, 2484
BseLI	CCNNNNNNNGG	8 cut(s)	127, 247, 530, 1381, 1636, 2491, 2554, 2555
BseMI	GCAATG	1 cut(s)	2577
BseMII	CTCAG	9 cut(s)	363, 714, 764, 798, 851, 1487, 1734, 2474, 2745
BseNI	ACTGG	6 cut(s)	241, 1457, 1710, 2474, 2489, 2620
BseRI	GAGGAG	6 cut(s)	41, 377, 389, 522, 1394, 1937
BseXI	GCAGC	6 cut(s)	960, 1879, 2526, 2579, 2665, 2684
BseYI	CCCAGC	4 cut(s)	386, 492, 1888, 2554
Bsh1285I	CGRYCG	1 cut(s)	280
BshFI	GGCC	6 cut(s)	888, 1278, 1386, 1607, 1818, 2097
BshNI	GGYRCC	3 cut(s)	193, 2184, 2449
BsiEI	CGRYCG	1 cut(s)	280
BsiHKAI	GWGCWC	2 cut(s)	231, 956
BsiHKCI	CYCGRG	2 cut(s)	615, 1819
BslFI	GGGAC	5 cut(s)	692, 808, 1191, 1212, 1505
BslI	CCNNNNNNNGG	8 cut(s)	127, 247, 530, 1381, 1636, 2491, 2554, 2555
BsmAI	GTCTC	2 cut(s)	258, 1816
BsmFI	GGGAC	5 cut(s)	692, 808, 1191, 1212, 1505
BsnI	GGCC	6 cut(s)	888, 1278, 1386, 1607, 1818, 2097
BsoBI	CYCGRG	2 cut(s)	615, 1819
Bsp119I	TTCGAA	2 cut(s)	440, 1475
Bsp1286I	GDGCHC	2 cut(s)	231, 956
Bsp13I	TCCGGA	3 cut(s)	154, 404, 1580
Bsp143I	GATC	9 cut(s)	82, 102, 267, 277, 1577, 1746, 2053, 2161, 2293
BspANI	GGCC	6 cut(s)	888, 1278, 1386, 1607, 1818, 2097
BspCNI	CTCAG	9 cut(s)	364, 715, 763, 799, 852, 1488, 1735, 2473, 2746
BspEI	TCCGGA	3 cut(s)	154, 404, 1580
BspMAI	CTGCAG	1 cut(s)	848
BspMI	ACCTGC	2 cut(s)	988, 1112
BspPI	GGATC	3 cut(s)	1572, 1585, 2061
BspT104I	TTCGAA	2 cut(s)	440, 1475
BspT107I	GGYRCC	3 cut(s)	193, 2184, 2449
BsrBI	CCGCTC	1 cut(s)	504
BsrDI	GCAATG	1 cut(s)	2577
BsrFI	RCCGGY	5 cut(s)	7, 172, 184, 340, 733
BsrI	ACTGG	6 cut(s)	241, 1457, 1710, 2474, 2489, 2620
BssAI	RCCGGY	5 cut(s)	7, 172, 184, 340, 733
BssECI	CCNNGG	3 cut(s)	123, 261, 630
BssMI	GATC	9 cut(s)	82, 102, 267, 277, 1577, 1746, 2053, 2161, 2293
BssNAI	GTATAC	1 cut(s)	1700
BssNI	GRCGYC	3 cut(s)	194, 344, 834
BssT1I	CCWWGG	1 cut(s)	630
Bst1107I	GTATAC	1 cut(s)	1700
Bst2UI	CCWGG	2 cut(s)	1134, 1989
Bst4CI	ACNGT	6 cut(s)	250, 334, 1314, 2127, 2525, 2628
Bst6I	CTCTTC	2 cut(s)	1722, 2706
BstACI	GRCGYC	3 cut(s)	194, 344, 834
BstBI	TTCGAA	2 cut(s)	440, 1475
BstC8I	GCNNGC	2 cut(s)	342, 2761
BstDSI	CCRYGG	1 cut(s)	123
BstEII	GGTNACC	1 cut(s)	1337
BstF5I	GGATG	7 cut(s)	966, 1311, 1369, 2314, 2374, 2485, 2713
BstH2I	RGCGCY	1 cut(s)	197
BstHHI	GCGC	2 cut(s)	37, 196
BstKTI	GATC	9 cut(s)	85, 105, 270, 280, 1580, 1749, 2056, 2164, 2296
BstMAI	GTCTC	2 cut(s)	258, 1816
BstMBI	GATC	9 cut(s)	82, 102, 267, 277, 1577, 1746, 2053, 2161, 2293
BstMCI	CGRYCG	1 cut(s)	280
BstMWI	GCNNNNNNNGC	7 cut(s)	20, 202, 518, 739, 970, 1949, 2727
BstNI	CCWGG	2 cut(s)	1134, 1989
BstPAI	GACNNNNGTC	1 cut(s)	145
BstPI	GGTNACC	1 cut(s)	1337
BstSCI	CCNGG	4 cut(s)	196, 1132, 1278, 1987
BstSFI	CTRYAG	3 cut(s)	844, 2339, 2746
BstV1I	GCAGC	6 cut(s)	960, 1879, 2526, 2579, 2665, 2684
BstV2I	GAAGAC	1 cut(s)	603
BstX2I	RGATCY	2 cut(s)	1577, 2053
BstXI	CCANNNNNNTGG	2 cut(s)	637, 1350
BstYI	RGATCY	2 cut(s)	1577, 2053
BstZ17I	GTATAC	1 cut(s)	1700
BsuI	GTATCC	2 cut(s)	1397, 2287
BsuRI	GGCC	6 cut(s)	888, 1278, 1386, 1607, 1818, 2097
BtgI	CCRYGG	1 cut(s)	123
BtgZI	GCGATG	1 cut(s)	660
BtrI	CACGTC	1 cut(s)	1715
BtsCI	GGATG	7 cut(s)	966, 1311, 1369, 2314, 2374, 2485, 2713
BtsI	GCAGTG	2 cut(s)	1451, 1684
BtsIMutI	CAGTG	8 cut(s)	804, 1334, 1451, 1464, 1684, 2468, 2496, 2751
BveI	ACCTGC	2 cut(s)	988, 1112
Cac8I	GCNNGC	2 cut(s)	342, 2761
CfoI	GCGC	2 cut(s)	37, 196
Cfr10I	RCCGGY	5 cut(s)	7, 172, 184, 340, 733
Cfr13I	GGNCC	7 cut(s)	65, 178, 1226, 1384, 1817, 2319, 2508
CseI	GACGC	2 cut(s)	333, 842
CsiI	ACCWGGT	1 cut(s)	1987
Csp6I	GTAC	6 cut(s)	40, 841, 1590, 2185, 2450, 2629
CviQI	GTAC	6 cut(s)	40, 841, 1590, 2185, 2450, 2629
DinI	GGCGCC	1 cut(s)	195
DpnI	GATC	9 cut(s)	84, 104, 269, 279, 1579, 1748, 2055, 2163, 2295
DpnII	GATC	9 cut(s)	82, 102, 267, 277, 1577, 1746, 2053, 2161, 2293
DraI	TTTAAA	1 cut(s)	2700
DriI	GACNNNNNGTC	1 cut(s)	607
EaeI	YGGCCR	1 cut(s)	1276
Eam1104I	CTCTTC	2 cut(s)	1722, 2706
Eam1105I	GACNNNNNGTC	1 cut(s)	607
EarI	CTCTTC	2 cut(s)	1722, 2706
EciI	GGCGGA	1 cut(s)	29
Ecl136II	GAGCTC	1 cut(s)	229
Eco130I	CCWWGG	1 cut(s)	630
Eco24I	GRGCYC	1 cut(s)	231
Eco47I	GGWCC	5 cut(s)	65, 178, 1226, 2319, 2508
Eco53kI	GAGCTC	1 cut(s)	229
Eco57I	CTGAAG	5 cut(s)	809, 1532, 2094, 2157, 2710
Eco88I	CYCGRG	2 cut(s)	615, 1819
Eco91I	GGTNACC	1 cut(s)	1337
EcoICRI	GAGCTC	1 cut(s)	229
EcoO109I	RGGNCCY	3 cut(s)	65, 1226, 2319
EcoO65I	GGTNACC	1 cut(s)	1337
EcoRII	CCWGG	2 cut(s)	1132, 1987
EcoT14I	CCWWGG	1 cut(s)	630
EcoT38I	GRGCYC	1 cut(s)	231
EgeI	GGCGCC	1 cut(s)	195
EheI	GGCGCC	1 cut(s)	195
ErhI	CCWWGG	1 cut(s)	630
FalI	AAGNNNNNCTT	2 cut(s)	1284, 1316
FaqI	GGGAC	5 cut(s)	692, 808, 1191, 1212, 1505
FauI	CCCGC	3 cut(s)	419, 667, 735
FauNDI	CATATG	2 cut(s)	1409, 2391
FbaI	TGATCA	2 cut(s)	2161, 2293
FblI	GTMKAC	5 cut(s)	111, 245, 1699, 2037, 2109
Fnu4HI	GCNGC	9 cut(s)	5, 129, 971, 974, 1893, 2515, 2568, 2654, 2673
FokI	GGATG	7 cut(s)	973, 1318, 1376, 2321, 2361, 2472, 2720
FriOI	GRGCYC	1 cut(s)	231
Fsp4HI	GCNGC	9 cut(s)	5, 129, 971, 974, 1893, 2515, 2568, 2654, 2673
FspBI	CTAG	5 cut(s)	798, 957, 1245, 1767, 2564
GlaI	GCGC	2 cut(s)	36, 195
GluI	GCNGC	9 cut(s)	5, 129, 971, 974, 1893, 2515, 2568, 2654, 2673
GsaI	CCCAGC	4 cut(s)	390, 496, 1892, 2558
HaeII	RGCGCY	1 cut(s)	197
HaeIII	GGCC	6 cut(s)	888, 1278, 1386, 1607, 1818, 2097
HgaI	GACGC	2 cut(s)	333, 842
HhaI	GCGC	2 cut(s)	37, 196
Hin1I	GRCGYC	3 cut(s)	194, 344, 834
Hin6I	GCGC	2 cut(s)	35, 194
HinP1I	GCGC	2 cut(s)	35, 194
HincII	GTYRAC	3 cut(s)	112, 246, 2023
HindII	GTYRAC	3 cut(s)	112, 246, 2023
HindIII	AAGCTT	1 cut(s)	1430
HphI	GGTGA	9 cut(s)	85, 277, 361, 476, 679, 748, 1012, 1366, 1880
Hpy166II	GTNNAC	9 cut(s)	112, 246, 640, 804, 1665, 1700, 2023, 2038, 2110
Hpy188III	TCNNGA	8 cut(s)	155, 405, 677, 1161, 1581, 1852, 2057, 2593
Hpy8I	GTNNAC	9 cut(s)	112, 246, 640, 804, 1665, 1700, 2023, 2038, 2110
Hpy99I	CGWCG	7 cut(s)	113, 140, 250, 304, 334, 349, 2131
HpyCH4III	ACNGT	6 cut(s)	250, 334, 1314, 2127, 2525, 2628
HpyCH4IV	ACGT	5 cut(s)	108, 215, 671, 1714, 2631
HpyCH4V	TGCA	8 cut(s)	822, 846, 1910, 2207, 2229, 2582, 2653, 2672
HpyF10VI	GCNNNNNNNGC	7 cut(s)	20, 202, 518, 739, 970, 1949, 2727
HpySE526I	ACGT	5 cut(s)	108, 215, 671, 1714, 2631
Hsp92I	GRCGYC	3 cut(s)	194, 344, 834
HspAI	GCGC	2 cut(s)	35, 194
KasI	GGCGCC	1 cut(s)	193
KflI	GGGWCCC	1 cut(s)	1226
Kpn2I	TCCGGA	3 cut(s)	154, 404, 1580
KpnI	GGTACC	2 cut(s)	2188, 2453
KroI	GCCGGC	1 cut(s)	340
KroNI	GCCGGC	1 cut(s)	342
Ksp22I	TGATCA	2 cut(s)	2161, 2293
Kzo9I	GATC	9 cut(s)	82, 102, 267, 277, 1577, 1746, 2053, 2161, 2293
LmnI	GCTCC	5 cut(s)	425, 501, 509, 526, 745
Lsp1109I	GCAGC	6 cut(s)	960, 1879, 2526, 2579, 2665, 2684
LweI	GCATC	7 cut(s)	867, 951, 1768, 2141, 2325, 2373, 2659
MabI	ACCWGGT	1 cut(s)	1987
MaeI	CTAG	5 cut(s)	798, 957, 1245, 1767, 2564
MaeII	ACGT	5 cut(s)	108, 215, 671, 1714, 2631
MalI	GATC	9 cut(s)	84, 104, 269, 279, 1579, 1748, 2055, 2163, 2295
MbiI	CCGCTC	1 cut(s)	504
MboI	GATC	9 cut(s)	82, 102, 267, 277, 1577, 1746, 2053, 2161, 2293
MflI	RGATCY	2 cut(s)	1577, 2053
MhlI	GDGCHC	2 cut(s)	231, 956
MluCI	AATT	6 cut(s)	410, 451, 1045, 1284, 1554, 1973
Mly113I	GGCGCC	1 cut(s)	194
MlyI	GAGTC	3 cut(s)	593, 1524, 2165
MmeI	TCCRAC	9 cut(s)	118, 281, 291, 300, 411, 1546, 2217, 2236, 2402
MreI	CGCCGGCG	1 cut(s)	340
MroI	TCCGGA	3 cut(s)	154, 404, 1580
MroNI	GCCGGC	1 cut(s)	340
MroXI	GAANNNNTTC	1 cut(s)	215
MseI	TTAA	4 cut(s)	2030, 2538, 2679, 2699
MslI	CAYNNNNRTG	3 cut(s)	1055, 1215, 2157
MspA1I	CMGCKG	2 cut(s)	662, 1892
MspR9I	CCNGG	4 cut(s)	198, 1134, 1280, 1989
MvaI	CCWGG	2 cut(s)	1134, 1989
MwoI	GCNNNNNNNGC	7 cut(s)	20, 202, 518, 739, 970, 1949, 2727
NaeI	GCCGGC	1 cut(s)	342
NarI	GGCGCC	1 cut(s)	194
NciI	CCSGG	2 cut(s)	198, 1280
NdeI	CATATG	2 cut(s)	1409, 2391
NdeII	GATC	9 cut(s)	82, 102, 267, 277, 1577, 1746, 2053, 2161, 2293
NgoMIV	GCCGGC	1 cut(s)	340
NmuCI	GTSAC	4 cut(s)	667, 830, 1449, 2465
NspV	TTCGAA	2 cut(s)	440, 1475
PaqCI	CACCTGC	1 cut(s)	988
PcsI	WCGNNNNNNNCGW	2 cut(s)	254, 326
PdiI	GCCGGC	1 cut(s)	342
PdmI	GAANNNNTTC	1 cut(s)	215
PfeI	GAWTC	8 cut(s)	117, 323, 553, 686, 1477, 1612, 1937, 2407
PflFI	GACNNNGTC	1 cut(s)	250
PflMI	CCANNNNNTGG	1 cut(s)	2491
PkrI	GCNGC	9 cut(s)	6, 130, 972, 975, 1894, 2516, 2569, 2655, 2674
Ple19I	CGATCG	1 cut(s)	280
PleI	GAGTC	3 cut(s)	592, 1523, 2165
PluTI	GGCGCC	1 cut(s)	197
PpsI	GAGTC	3 cut(s)	592, 1523, 2165
PpuMI	RGGWCCY	3 cut(s)	65, 1226, 2319
PshAI	GACNNNNGTC	1 cut(s)	145
PshBI	ATTAAT	1 cut(s)	2538
Psp124BI	GAGCTC	1 cut(s)	231
Psp1406I	AACGTT	1 cut(s)	215
Psp5II	RGGWCCY	3 cut(s)	65, 1226, 2319
Psp6I	CCWGG	2 cut(s)	1132, 1987
PspEI	GGTNACC	1 cut(s)	1337
PspFI	CCCAGC	4 cut(s)	386, 492, 1888, 2554
PspGI	CCWGG	2 cut(s)	1132, 1987
PspPI	GGNCC	7 cut(s)	65, 178, 1226, 1384, 1817, 2319, 2508
PspPPI	RGGWCCY	3 cut(s)	65, 1226, 2319
PstI	CTGCAG	1 cut(s)	848
PsuI	RGATCY	2 cut(s)	1577, 2053
PsyI	GACNNNGTC	1 cut(s)	250
PvuI	CGATCG	1 cut(s)	280
PvuII	CAGCTG	1 cut(s)	1892
RsaI	GTAC	6 cut(s)	41, 842, 1591, 2186, 2451, 2630
RsaNI	GTAC	6 cut(s)	40, 841, 1590, 2185, 2450, 2629
RseI	CAYNNNNRTG	3 cut(s)	1055, 1215, 2157
SacI	GAGCTC	1 cut(s)	231
SalI	GTCGAC	2 cut(s)	110, 244
SaqAI	TTAA	4 cut(s)	2030, 2538, 2679, 2699
SatI	GCNGC	9 cut(s)	5, 129, 971, 974, 1893, 2515, 2568, 2654, 2673
Sau3AI	GATC	9 cut(s)	82, 102, 267, 277, 1577, 1746, 2053, 2161, 2293
Sau96I	GGNCC	7 cut(s)	65, 178, 1226, 1384, 1817, 2319, 2508
ScaI	AGTACT	3 cut(s)	41, 842, 1591
SchI	GAGTC	3 cut(s)	593, 1524, 2165
ScrFI	CCNGG	4 cut(s)	198, 1134, 1280, 1989
SduI	GDGCHC	2 cut(s)	231, 956
SexAI	ACCWGGT	1 cut(s)	1987
SfaNI	GCATC	7 cut(s)	867, 951, 1768, 2141, 2325, 2373, 2659
SfcI	CTRYAG	3 cut(s)	844, 2339, 2746
SfoI	GGCGCC	1 cut(s)	195
SfuI	TTCGAA	2 cut(s)	440, 1475
SgrAI	CRCCGGYG	2 cut(s)	7, 340
SinI	GGWCC	5 cut(s)	65, 178, 1226, 2319, 2508
SmiMI	CAYNNNNRTG	3 cut(s)	1055, 1215, 2157
SmlI	CTYRAG	5 cut(s)	230, 965, 1850, 2281, 2429
SmoI	CTYRAG	5 cut(s)	230, 965, 1850, 2281, 2429
Sse9I	AATT	6 cut(s)	410, 451, 1045, 1284, 1554, 1973
SspDI	GGCGCC	1 cut(s)	193
SspI	AATATT	1 cut(s)	2684
SspMI	CTAG	5 cut(s)	798, 957, 1245, 1767, 2564
SstI	GAGCTC	1 cut(s)	231
StyD4I	CCNGG	4 cut(s)	196, 1132, 1278, 1987
StyI	CCWWGG	1 cut(s)	630
TaaI	ACNGT	6 cut(s)	250, 334, 1314, 2127, 2525, 2628
TaiI	ACGT	5 cut(s)	111, 218, 674, 1717, 2634
TaqI	TCGA	7 cut(s)	111, 245, 276, 360, 440, 875, 1475
TaqII	GACCGA	1 cut(s)	2496
TasI	AATT	6 cut(s)	410, 451, 1045, 1284, 1554, 1973
TatI	WGTACW	3 cut(s)	39, 840, 1589
TauI	GCSGC	3 cut(s)	7, 131, 973
TfiI	GAWTC	8 cut(s)	117, 323, 553, 686, 1477, 1612, 1937, 2407
Tru1I	TTAA	4 cut(s)	2030, 2538, 2679, 2699
Tru9I	TTAA	4 cut(s)	2030, 2538, 2679, 2699
TscAI	CASTG	8 cut(s)	811, 1334, 1458, 1464, 1684, 2468, 2496, 2758
TseFI	GTSAC	4 cut(s)	667, 830, 1449, 2465
TseI	GCWGC	6 cut(s)	973, 1892, 2514, 2567, 2653, 2672
Tsp45I	GTSAC	4 cut(s)	667, 830, 1449, 2465
TspDTI	ATGAA	5 cut(s)	224, 1019, 1996, 2076, 2724
TspGWI	ACGGA	2 cut(s)	577, 597
TspRI	CASTG	8 cut(s)	811, 1334, 1458, 1464, 1684, 2468, 2496, 2758
Tth111I	GACNNNGTC	1 cut(s)	250
Van91I	CCANNNNNTGG	1 cut(s)	2491
VpaK11BI	GGWCC	5 cut(s)	65, 178, 1226, 2319, 2508
VspI	ATTAAT	1 cut(s)	2538
XapI	RAATTY	1 cut(s)	1554
XcmI	CCANNNNNNNNNTGG	3 cut(s)	634, 1140, 1217
XmiI	GTMKAC	5 cut(s)	111, 245, 1699, 2037, 2109
XmnI	GAANNNNTTC	1 cut(s)	215
XspI	CTAG	5 cut(s)	798, 957, 1245, 1767, 2564
ZrmI	AGTACT	3 cut(s)	41, 842, 1591

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.