Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012855

Physical Location & Seq

Genomic Coordinates Reverse (-)

29797565 .. 29801352

Gene Structure 3788 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1304900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3045 bp

ATGCGGCTCCTTTTCCTCCTCTTCCTCCTCCACCTCCACCGCTCCCTCTCCGCGGGCGCAATGTCCGACTACCGCGCCCTCCTCTCCCTCAAATCTTCCATAACGTCCGACCCCAACTCGGCCCTTTCCACCTGGACCCCCACCACCAGCCACTGCACTTGGTCCGGCGTTACCTGCGACTCCTCCCGCCGCTACGTGACCTCCCTTGACCTCTCCGGCCTCGACCTCACCGGCACTCTTTCTCCTGACCTCGCCCACCTCCGCTTCCTCTCCAACCTCACCCTCGCCGAGAACCAGTTCTCGGGTCCCATCCCGCCCGATATCTCAGCCCTCTCGGGCCTCCGCCTCCTCAACCTCTCCAACAACGTCTTCAACACCACCTTCCCCCGTCAGCTCTCCAACCTCACCCGCCTCACCGTCCTCGACCTCTACAACAACAACCTCACCGGCAAACTCCCCGTCTCCGTCACCCACATGACCTCCCTCCGCCATTTGCATTTGGGCGGAAACTTCTTCTCTGGCCAGATCCCTCCCGAGTTCGGCCGCTTCCCATTCCTCGAGTACCTAGCCGTCTCCGGCAACGAATTGGGCGGTTCAATCCCTCCCGAAATCGGAAACTTAACCACCTTGAAAGAGCTCTACATCGGCTATTTCAACAGCTACGAAGGCGGAATACCGCCCGAAATTGGAAACTTATCCAACCTCGTCCGGTTCGACGCCGCCAACTGTAACTTAACCGGAGAGGTCCCGCCGGAACTGGGTCGGCTGCAAAATGTCGACACCCTGTTTCTCCAGGTCAATGCCCTTTCCGGGTGGTTGACTCCCGAGCTGGGCTACCTCAAGAGCCTGAAATCCATGGACTTATCCAACAATATGTATACAGGCGAGATTCCGGGTTCCTTTTCGGAGCTCAAGAACTTGACCCTTTTGAATCTCTTCCGAAACAGACTCCACGGCGCCATTCCGGAGTTTATCGGCGACTTGCCGGAGCTCCAGGTTCTGCAGCTCTGGGAGAACAACTTCACTGGCAGCATCCCTCAGGGTTTGGGCAAAAACGGGAAGCTTCAAATCCTCGACCTTTCGTCAAACAAGTTGACCGGAACTCTCCCTCCCGATATGTGTTTCGGGAACAGTCTCCAGACCCTCATCACTCTCTCCAACTTCCTTTTCGGGCCGATTCCCGAGTCGCTTGGCCGGTGTGACTCGCTGAGTCGGATCCGAATGGGCGACAACTTCCTCAACGGGTCGATTCCGAAAGGTCTGTTTGGTCTGCCCAAGCTTACCCAAGTCGAGTTGCAGGATAATCTCTTGGATGGTTCGTTTCCCGAGACCGATACAATCTCGGGGAATCTCGGCCAGATTAGTCTTTCCAATAACCGGCTTTCCGGGTCCCTGCCACCTACCATCGGCAACTTCTCCGGCGTCCAGAAACTTCTTCTCGACGGGAACAAGTTCTCGGGTCGAATCCCACCGGAGATTGGGCGATTGCAGCAGCTATCCAAGATGGATTTCAAGCACAACAAGTTCATGGGTTCGATAACCCCCGAAATCAGCCTATGCAAGCTCTTGACATTTGTCGATCTCAGCCGAAACGAGCTCTCCGGCGAGATTCCGAAGGAGATTACCGGTATGAGGATACTGAACTACCTCAACCTTTCGAGAAACCATCTTGTTGGCAGCATTCCGTCGTCTATATCCACAATGCAGAGCTTGACATCCGTGGACTTTTCATATAACAATCTTTCGGGTTTGGTTCCGGGCACTGGTCAGTTCAGTTACTTCAATTACACTTCGTTTTTGGGCAACCCCGACCTCTGCGGTCCCTATTTGGTTCCCTGCAAAGACGGGGTGGCCAATGGCACTCACCAACCACATGTAAAAGGTGCACTCACTGCTTCTCTGAAGCTATTGCTTGTTATTGGGTTGCTTCTGTGCTCGATCATATTCGCGGTTGCAGCAATAATCAAGGCCCGGTCATTGAAGAGAGCAAGCGATTCCCGAGCTTGGAAACTTACTGCATTCCAACGCTTGGACTTCTCGGTTGATGATGTGTTGGATTCGTTGAAGGAGGACAACATTATTGGGAAAGGAGGGGCTGGGATTGTGTACAAAGGAGCTATGCCTACTGGTGACAGTGTGGCAGTCAAAAGACTCCCTGCAATGAGCAGAGGGTCCTCCCACGACCACGGATTCAACGCCGAAATCCAAACTCTTGGTCGAATTCGACACAGACACATTGTTCGATTATTGGGTTTCTGTTCAAACCACGAAACAAATCTTTTGGTTTATGAGTACATGCCCAATGGGAGCTTGGGTGAAGTTCTTCATGGCAAAAAAGGGGGTCACTTGCATTGGGACACAAGGTACAAAATTGCCATTGAAGCCGCAAAGGGGCTTTGCTATCTTCACCACGACTGCTCACCGTTGATCGTCCACAGAGACGTGAAGTCGAATAACATCCTCCTCGACTCTAATTTCGAAGCTCATGTAGCTGATTTCGGGCTTGCCAAGTTCCTGCAGGATTCTGGCACGTCCGAGTGCATGTCTGCAATTGCTGGTTCATATGGATATATTGCTCCAGAGTATGCATACACATTGAAGGTTGATGAGAAGAGCGACGTTTATAGCTTCGGTGTGGTTCTCTTGGAACTTGTTAGCGGGAGGAAACCGGTTGGTGAATTTGGGGACGGAGTGGACATAGTCCAATGGGTGCGAAAAATGACTGATTCAAACAAGGAAGGAGTCCTCAAAATCCTTGACCCTAGACTCCCTTCAGTTCCTCTCCATGAGGTTATGCACGTATTTTACGTGGCAATGCTGTGTGTGGAAGAACAAGCAGTGGAACGTCCAACAATGCGAGAAGTGGTTCAAATTCTGACCGAGCTTCCAAAGGGGCCCGGCTCAAAACTGGTAGAGGAATCAGCCATCACAGAGTCCTCGCCTCCACCTGCCACTGCTGCATCGGAGTCTCCAGGTACAACGTCAAAAGACACGAAAGACCAGCCACCACCGCAGTCACTGCCGCCCGATCTTCTTAGCATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009908 GO:0010073 GO:0010075 GO:0016020 GO:0022414 GO:0032501 GO:0032502 GO:0033612 GO:0040008 GO:0043621 GO:0044464 GO:0048229 GO:0048367 GO:0048437 GO:0048507 GO:0048509 GO:0048608 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0061458 GO:0065007 GO:0071944 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1015

Amino Acids

110.62

Weight (kDa)

6.25

Isoelectric Point (pI)

34.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	22 - 60	6.3e-13	Leucine rich repeat N-terminal domain
LRR_14	PF23598	82 - 168	1.7e-06	Leucine-rich repeat region
LRR_8	PF13855	113 - 170	1.7e-07	Leucine rich repeat
LRR_14	PF23598	157 - 267	7.1e-08	Leucine-rich repeat region
LRR_14	PF23598	273 - 360	8.4e-06	Leucine-rich repeat region
PK_Tyr_Ser-Thr	PF07714	690 - 959	6.2e-45	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	690 - 957	1.4e-44	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0012855)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49670 AT5G65700 AT5G65700
fragaria_vesca	FvH4_1g18710
malus_domestica	MD02G1194600.v1.1 MD15G1304900.v1.1
prunus_persica	Prupe.6G212200_v2.0.a1
pyrus_communis	pycom02g15850 pycom15g26730
rosa_chinensis	RchiOBHm_Chr2g0109581
rosa_laevigata	RLG00000017747
rosa_multiflora	Rmu_sc0000332.1_g000045
rosa_roxburghii	Rroxscaffold_2G00134130
rosa_rugosa	Rorug02G0160800
rosa_samantha	Rh2AG213200 Rh2BG223400 Rh2CG215200 Rh2DG218700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2956
Acc36I	ACCTGC	2 cut(s)	182, 2956
AccB1I	GGYRCC	1 cut(s)	956
AccB7I	CCANNNNNTGG	1 cut(s)	2029
AccBSI	CCGCTC	1 cut(s)	42
AccI	GTMKAC	2 cut(s)	777, 878
AccII	CGCG	3 cut(s)	53, 75, 1949
AccIII	TCCGGA	1 cut(s)	964
AclWI	GGATC	3 cut(s)	520, 1210, 1223
AcoI	YGGCCR	5 cut(s)	520, 541, 1192, 1354, 1851
AcsI	RAATTY	3 cut(s)	2220, 2678, 2871
AcuI	CTGAAG	2 cut(s)	1922, 2757
AcyI	GRCGYC	3 cut(s)	717, 957, 1422
AfaI	GTAC	5 cut(s)	563, 2108, 2294, 2366, 2977
AflIII	ACRYGT	1 cut(s)	1873
AgeI	ACCGGT	2 cut(s)	1625, 2668
AhdI	GACNNNNNGTC	2 cut(s)	1081, 2446
AjiI	CACGTC	2 cut(s)	2443, 2532
AjnI	CCWGG	4 cut(s)	131, 792, 993, 2971
AjuI	GAANNNNNNNTTGG	6 cut(s)	353, 385, 1151, 1183, 1493, 1525
AloI	GAACNNNNNNTCC	2 cut(s)	1093, 1125
Alw21I	GWGCWC	6 cut(s)	639, 912, 993, 1599, 1888, 1937
Alw26I	GTCTC	6 cut(s)	466, 577, 1139, 1322, 2433, 2973
Alw44I	GTGCAC	1 cut(s)	1884
AlwI	GGATC	3 cut(s)	520, 1210, 1223
AlwNI	CAGNNNCTG	3 cut(s)	153, 1000, 3019
Aor13HI	TCCGGA	1 cut(s)	964
ApaI	GGGCCC	1 cut(s)	2898
ApaLI	GTGCAC	1 cut(s)	1884
ApeKI	GCWGC	8 cut(s)	766, 1003, 1029, 1489, 1492, 1677, 1955, 2957
ApoI	RAATTY	3 cut(s)	2220, 2678, 2871
ArsI	GACNNNNNNTTYG	8 cut(s)	444, 476, 2200, 2223, 2232, 2255, 2459, 2491
AsiGI	ACCGGT	2 cut(s)	1625, 2668
Asp700I	GAANNNNTTC	6 cut(s)	296, 935, 1019, 1451, 2322, 2865
AspLEI	GCGC	3 cut(s)	59, 77, 959
AsuC2I	CCSGG	6 cut(s)	811, 894, 1387, 1758, 1972, 2898
AsuII	TTCGAA	1 cut(s)	2478
AvaII	GGWCC	7 cut(s)	135, 162, 305, 745, 1389, 1820, 2172
AxyI	CCTNAGG	1 cut(s)	1038
BaeGI	GKGCMC	3 cut(s)	1763, 1888, 2898
BalI	TGGCCA	2 cut(s)	522, 1853
BamHI	GGATCC	1 cut(s)	1215
BanI	GGYRCC	1 cut(s)	956
BanII	GRGCYC	5 cut(s)	639, 912, 993, 1599, 2898
BarI	GAAGNNNNNNTAC	2 cut(s)	657, 689
BbsI	GAAGAC	1 cut(s)	361
Bbv12I	GWGCWC	6 cut(s)	639, 912, 993, 1599, 1888, 1937
BbvI	GCAGC	8 cut(s)	753, 1015, 1041, 1501, 1504, 1689, 1967, 2944
BccI	CCATC	6 cut(s)	317, 1307, 1412, 1498, 1674, 2933
BceAI	ACGGC	2 cut(s)	554, 970
BciT130I	CCWGG	4 cut(s)	133, 794, 995, 2973
BciVI	GTATCC	1 cut(s)	1629
BcnI	CCSGG	6 cut(s)	811, 894, 1387, 1758, 1972, 2898
BcoDI	GTCTC	6 cut(s)	466, 577, 1139, 1322, 2433, 2973
BfaI	CTAG	2 cut(s)	566, 2763
BfmI	CTRYAG	2 cut(s)	1001, 2516
BfoI	RGCGCY	1 cut(s)	960
BfuAI	ACCTGC	2 cut(s)	182, 2956
BfuI	GTATCC	1 cut(s)	1629
Bme18I	GGWCC	7 cut(s)	135, 162, 305, 745, 1389, 1820, 2172
BmeRI	GACNNNNNGTC	2 cut(s)	1081, 2446
BmgBI	CACGTC	2 cut(s)	2443, 2532
BmrI	ACTGGG	1 cut(s)	767
BmsI	GCATC	2 cut(s)	1041, 2969
BmuI	ACTGGG	1 cut(s)	767
BoxI	GACNNNNGTC	1 cut(s)	1574
BpiI	GAAGAC	1 cut(s)	361
BpmI	CTGGAG	5 cut(s)	776, 977, 1121, 2562, 2955
Bpu14I	TTCGAA	1 cut(s)	2478
BpuEI	CTTGAG	2 cut(s)	824, 896
BpuMI	CCSGG	6 cut(s)	811, 894, 1387, 1758, 1972, 2898
BsaAI	YACGTR	3 cut(s)	196, 2800, 2809
BsaHI	GRCGYC	3 cut(s)	717, 957, 1422
BsaI	GGTCTC	1 cut(s)	1322
BsaJI	CCNNGG	5 cut(s)	51, 855, 952, 1719, 2185
BsaWI	WCCGGW	7 cut(s)	708, 737, 964, 1097, 1471, 1625, 2668
Bse118I	RCCGGY	6 cut(s)	230, 446, 1194, 1377, 1625, 2668
Bse1I	ACTGG	6 cut(s)	295, 762, 1030, 1768, 2131, 2913
Bse21I	CCTNAGG	1 cut(s)	1038
Bse3DI	GCAATG	3 cut(s)	66, 2166, 2820
BseAI	TCCGGA	1 cut(s)	964
BseBI	CCWGG	4 cut(s)	133, 794, 995, 2973
BseDI	CCNNGG	5 cut(s)	51, 855, 952, 1719, 2185
BseGI	GGATG	5 cut(s)	309, 1032, 1318, 1715, 2457
BseMI	GCAATG	3 cut(s)	66, 2166, 2820
BseMII	CTCAG	4 cut(s)	339, 1052, 1199, 1597
BseNI	ACTGG	6 cut(s)	295, 762, 1030, 1768, 2131, 2913
BseRI	GAGGAG	6 cut(s)	8, 17, 71, 172, 338, 2453
BseSI	GKGCMC	3 cut(s)	1763, 1888, 2898
BseX3I	CGGCCG	1 cut(s)	541
BseXI	GCAGC	8 cut(s)	753, 1015, 1041, 1501, 1504, 1689, 1967, 2944
BseYI	CCCAGC	2 cut(s)	829, 2096
BsgI	GTGCAG	1 cut(s)	139
Bsh1236I	CGCG	3 cut(s)	53, 75, 1949
Bsh1285I	CGRYCG	1 cut(s)	544
BshNI	GGYRCC	1 cut(s)	956
BshTI	ACCGGT	2 cut(s)	1625, 2668
BsiEI	CGRYCG	1 cut(s)	544
BsiHKAI	GWGCWC	6 cut(s)	639, 912, 993, 1599, 1888, 1937
BslFI	GGGAC	6 cut(s)	291, 731, 1375, 1806, 2369, 2699
BsmAI	GTCTC	6 cut(s)	466, 577, 1139, 1322, 2433, 2973
BsmBI	CGTCTC	3 cut(s)	466, 577, 2433
BsmFI	GGGAC	6 cut(s)	291, 731, 1375, 1806, 2369, 2699
BsmI	GAATGC	2 cut(s)	1680, 2018
Bso31I	GGTCTC	1 cut(s)	1322
Bsp119I	TTCGAA	1 cut(s)	2478
Bsp120I	GGGCCC	1 cut(s)	2894
Bsp1286I	GDGCHC	8 cut(s)	639, 912, 993, 1599, 1763, 1888, 1937, 2898
Bsp13I	TCCGGA	1 cut(s)	964
Bsp1407I	TGTACA	1 cut(s)	2106
Bsp143I	GATC	6 cut(s)	525, 1215, 1579, 1938, 2427, 3028
Bsp19I	CCATGG	1 cut(s)	855
BspCNI	CTCAG	4 cut(s)	338, 1051, 1200, 1596
BspEI	TCCGGA	1 cut(s)	964
BspFNI	CGCG	3 cut(s)	53, 75, 1949
BspMAI	CTGCAG	2 cut(s)	1005, 2520
BspMI	ACCTGC	2 cut(s)	182, 2956
BspPI	GGATC	3 cut(s)	520, 1210, 1223
BspQI	GCTCTTC	1 cut(s)	2606
BspT104I	TTCGAA	1 cut(s)	2478
BspT107I	GGYRCC	1 cut(s)	956
BspTNI	GGTCTC	1 cut(s)	1322
BsrBI	CCGCTC	1 cut(s)	42
BsrDI	GCAATG	3 cut(s)	66, 2166, 2820
BsrFI	RCCGGY	6 cut(s)	230, 446, 1194, 1377, 1625, 2668
BsrGI	TGTACA	1 cut(s)	2106
BsrI	ACTGG	6 cut(s)	295, 762, 1030, 1768, 2131, 2913
BssAI	RCCGGY	6 cut(s)	230, 446, 1194, 1377, 1625, 2668
BssECI	CCNNGG	5 cut(s)	51, 855, 952, 1719, 2185
BssMI	GATC	6 cut(s)	525, 1215, 1579, 1938, 2427, 3028
BssNAI	GTATAC	1 cut(s)	879
BssNI	GRCGYC	3 cut(s)	717, 957, 1422
BssT1I	CCWWGG	1 cut(s)	855
Bst1107I	GTATAC	1 cut(s)	879
Bst2UI	CCWGG	4 cut(s)	133, 794, 995, 2973
Bst4CI	ACNGT	5 cut(s)	418, 728, 1133, 2135, 2424
Bst6I	CTCTTC	4 cut(s)	26, 941, 1976, 2606
BstACI	GRCGYC	3 cut(s)	717, 957, 1422
BstAPI	GCANNNNNTGC	2 cut(s)	1892, 3019
BstAUI	TGTACA	1 cut(s)	2106
BstBAI	YACGTR	3 cut(s)	196, 2800, 2809
BstBI	TTCGAA	1 cut(s)	2478
BstC8I	GCNNGC	4 cut(s)	55, 1562, 1990, 2505
BstDEI	CTNAG	5 cut(s)	325, 1038, 1208, 1583, 3035
BstDSI	CCRYGG	5 cut(s)	51, 855, 952, 1719, 2185
BstF5I	GGATG	5 cut(s)	309, 1032, 1318, 1715, 2457
BstFNI	CGCG	3 cut(s)	53, 75, 1949
BstH2I	RGCGCY	1 cut(s)	960
BstHHI	GCGC	3 cut(s)	59, 77, 959
BstKTI	GATC	6 cut(s)	528, 1218, 1582, 1941, 2430, 3031
BstMAI	GTCTC	6 cut(s)	466, 577, 1139, 1322, 2433, 2973
BstMBI	GATC	6 cut(s)	525, 1215, 1579, 1938, 2427, 3028
BstMCI	CGRYCG	1 cut(s)	544
BstNI	CCWGG	4 cut(s)	133, 794, 995, 2973
BstNSI	RCATGY	3 cut(s)	1877, 2299, 2545
BstPAI	GACNNNNGTC	1 cut(s)	1574
BstSFI	CTRYAG	2 cut(s)	1001, 2516
BstSLI	GKGCMC	3 cut(s)	1763, 1888, 2898
BstUI	CGCG	3 cut(s)	53, 75, 1949
BstV1I	GCAGC	8 cut(s)	753, 1015, 1041, 1501, 1504, 1689, 1967, 2944
BstV2I	GAAGAC	1 cut(s)	361
BstX2I	RGATCY	2 cut(s)	525, 1215
BstXI	CCANNNNNNTGG	2 cut(s)	1673, 2213
BstYI	RGATCY	2 cut(s)	525, 1215
BstZ17I	GTATAC	1 cut(s)	879
BstZI	CGGCCG	1 cut(s)	541
Bsu36I	CCTNAGG	1 cut(s)	1038
BsuI	GTATCC	1 cut(s)	1629
BtgI	CCRYGG	5 cut(s)	51, 855, 952, 1719, 2185
BtrI	CACGTC	2 cut(s)	2443, 2532
BtsCI	GGATG	5 cut(s)	309, 1032, 1318, 1715, 2457
BtsI	GCAGTG	5 cut(s)	151, 1890, 2844, 2952, 3017
BtsIMutI	CAGTG	8 cut(s)	151, 1023, 1761, 1890, 2140, 2844, 2952, 3017
BveI	ACCTGC	2 cut(s)	182, 2956
Cac8I	GCNNGC	4 cut(s)	55, 1562, 1990, 2505
CaiI	CAGNNNCTG	3 cut(s)	153, 1000, 3019
CfoI	GCGC	3 cut(s)	59, 77, 959
Cfr10I	RCCGGY	6 cut(s)	230, 446, 1194, 1377, 1625, 2668
Cfr42I	CCGCGG	1 cut(s)	54
CseI	GACGC	2 cut(s)	725, 1411
Csp6I	GTAC	5 cut(s)	562, 2107, 2293, 2365, 2976
CspAI	ACCGGT	2 cut(s)	1625, 2668
CspCI	CAANNNNNGTGG	2 cut(s)	140, 175
CviAII	CATG	9 cut(s)	475, 856, 1528, 1874, 2296, 2327, 2486, 2542, 2786
CviQI	GTAC	5 cut(s)	562, 2107, 2293, 2365, 2976
DdeI	CTNAG	5 cut(s)	325, 1038, 1208, 1583, 3035
DinI	GGCGCC	1 cut(s)	958
DpnI	GATC	6 cut(s)	527, 1217, 1581, 1940, 2429, 3030
DpnII	GATC	6 cut(s)	525, 1215, 1579, 1938, 2427, 3028
DriI	GACNNNNNGTC	2 cut(s)	1081, 2446
EaeI	YGGCCR	5 cut(s)	520, 541, 1192, 1354, 1851
EagI	CGGCCG	1 cut(s)	541
Eam1104I	CTCTTC	4 cut(s)	26, 941, 1976, 2606
Eam1105I	GACNNNNNGTC	2 cut(s)	1081, 2446
EarI	CTCTTC	4 cut(s)	26, 941, 1976, 2606
EciI	GGCGGA	4 cut(s)	332, 476, 519, 684
Ecl136II	GAGCTC	4 cut(s)	637, 910, 991, 1597
EclXI	CGGCCG	1 cut(s)	541
Eco130I	CCWWGG	1 cut(s)	855
Eco24I	GRGCYC	5 cut(s)	639, 912, 993, 1599, 2898
Eco31I	GGTCTC	1 cut(s)	1322
Eco32I	GATATC	1 cut(s)	322
Eco47I	GGWCC	7 cut(s)	135, 162, 305, 745, 1389, 1820, 2172
Eco52I	CGGCCG	1 cut(s)	541
Eco53kI	GAGCTC	4 cut(s)	637, 910, 991, 1597
Eco57I	CTGAAG	2 cut(s)	1922, 2757
Eco81I	CCTNAGG	1 cut(s)	1038
EcoICRI	GAGCTC	4 cut(s)	637, 910, 991, 1597
EcoO109I	RGGNCCY	5 cut(s)	305, 745, 1389, 2172, 2894
EcoRI	GAATTC	1 cut(s)	2220
EcoRII	CCWGG	4 cut(s)	131, 792, 993, 2971
EcoRV	GATATC	1 cut(s)	322
EcoT14I	CCWWGG	1 cut(s)	855
EcoT22I	ATGCAT	1 cut(s)	2590
EcoT38I	GRGCYC	5 cut(s)	639, 912, 993, 1599, 2898
EgeI	GGCGCC	1 cut(s)	958
EheI	GGCGCC	1 cut(s)	958
ErhI	CCWWGG	1 cut(s)	855
Esp3I	CGTCTC	3 cut(s)	466, 577, 2433
FaeI	CATG	9 cut(s)	478, 859, 1531, 1877, 2299, 2330, 2489, 2545, 2789
FaqI	GGGAC	6 cut(s)	291, 731, 1375, 1806, 2369, 2699
FatI	CATG	9 cut(s)	474, 855, 1527, 1873, 2295, 2326, 2485, 2541, 2785
FauI	CCCGC	6 cut(s)	46, 194, 321, 416, 756, 2651
FauNDI	CATATG	2 cut(s)	2563, 3041
FblI	GTMKAC	2 cut(s)	777, 878
FokI	GGATG	5 cut(s)	296, 1019, 1325, 1702, 2444
FriOI	GRGCYC	5 cut(s)	639, 912, 993, 1599, 2898
FspBI	CTAG	2 cut(s)	566, 2763
GlaI	GCGC	3 cut(s)	58, 76, 958
GsaI	CCCAGC	2 cut(s)	833, 2100
GsuI	CTGGAG	5 cut(s)	776, 977, 1121, 2562, 2955
HaeII	RGCGCY	1 cut(s)	960
HgaI	GACGC	2 cut(s)	725, 1411
HhaI	GCGC	3 cut(s)	59, 77, 959
Hin1I	GRCGYC	3 cut(s)	717, 957, 1422
Hin1II	CATG	9 cut(s)	478, 859, 1531, 1877, 2299, 2330, 2489, 2545, 2789
Hin6I	GCGC	3 cut(s)	57, 75, 957
HinP1I	GCGC	3 cut(s)	57, 75, 957
HincII	GTYRAC	3 cut(s)	778, 819, 1095
HindII	GTYRAC	3 cut(s)	778, 819, 1095
HindIII	AAGCTT	2 cut(s)	1061, 1277
Hpy166II	GTNNAC	9 cut(s)	778, 819, 879, 1095, 1723, 1886, 2107, 2434, 2695
Hpy8I	GTNNAC	9 cut(s)	778, 819, 879, 1095, 1723, 1886, 2107, 2434, 2695
Hpy99I	CGWCG	4 cut(s)	719, 1445, 1690, 2621
HpyAV	CCTTC	7 cut(s)	391, 659, 1609, 2059, 2593, 2732, 2781
HpyCH4III	ACNGT	5 cut(s)	418, 728, 1133, 2135, 2424
HpyF3I	CTNAG	5 cut(s)	325, 1038, 1208, 1583, 3035
Hsp92I	GRCGYC	3 cut(s)	717, 957, 1422
Hsp92II	CATG	9 cut(s)	478, 859, 1531, 1877, 2299, 2330, 2489, 2545, 2789
HspAI	GCGC	3 cut(s)	57, 75, 957
KasI	GGCGCC	1 cut(s)	956
KflI	GGGWCCC	2 cut(s)	305, 1389
Kpn2I	TCCGGA	1 cut(s)	964
KspI	CCGCGG	1 cut(s)	54
Kzo9I	GATC	6 cut(s)	525, 1215, 1579, 1938, 2427, 3028
LguI	GCTCTTC	1 cut(s)	2606
LmnI	GCTCC	8 cut(s)	12, 47, 907, 988, 996, 2114, 2307, 2581
Lsp1109I	GCAGC	8 cut(s)	753, 1015, 1041, 1501, 1504, 1689, 1967, 2944
LweI	GCATC	2 cut(s)	1041, 2969
MaeI	CTAG	2 cut(s)	566, 2763
MaeIII	GTNAC	9 cut(s)	169, 196, 466, 728, 1199, 1775, 2129, 2342, 3015
MalI	GATC	6 cut(s)	527, 1217, 1581, 1940, 2429, 3030
MbiI	CCGCTC	1 cut(s)	42
MboI	GATC	6 cut(s)	525, 1215, 1579, 1938, 2427, 3028
MfeI	CAATTG	1 cut(s)	2550
MflI	RGATCY	2 cut(s)	525, 1215
MhlI	GDGCHC	8 cut(s)	639, 912, 993, 1599, 1763, 1888, 1937, 2898
MlsI	TGGCCA	2 cut(s)	522, 1853
MluCI	AATT	9 cut(s)	584, 684, 1783, 2220, 2370, 2473, 2550, 2678, 2871
MluNI	TGGCCA	2 cut(s)	522, 1853
Mly113I	GGCGCC	1 cut(s)	957
Mox20I	TGGCCA	2 cut(s)	522, 1853
Mph1103I	ATGCAT	1 cut(s)	2590
MroI	TCCGGA	1 cut(s)	964
MroXI	GAANNNNTTC	6 cut(s)	296, 935, 1019, 1451, 2322, 2865
MscI	TGGCCA	2 cut(s)	522, 1853
MseI	TTAA	2 cut(s)	620, 734
MslI	CAYNNNNRTG	1 cut(s)	473
Msp20I	TGGCCA	2 cut(s)	522, 1853
MspA1I	CMGCKG	1 cut(s)	53
MunI	CAATTG	1 cut(s)	2550
Mva1269I	GAATGC	2 cut(s)	1680, 2018
MvaI	CCWGG	4 cut(s)	133, 794, 995, 2973
MvnI	CGCG	3 cut(s)	53, 75, 1949
NarI	GGCGCC	1 cut(s)	957
NciI	CCSGG	6 cut(s)	811, 894, 1387, 1758, 1972, 2898
NcoI	CCATGG	1 cut(s)	855
NdeI	CATATG	2 cut(s)	2563, 3041
NdeII	GATC	6 cut(s)	525, 1215, 1579, 1938, 2427, 3028
NlaIII	CATG	9 cut(s)	478, 859, 1531, 1877, 2299, 2330, 2489, 2545, 2789
NmeAIII	GCCGAG	3 cut(s)	98, 313, 1332
NmuCI	GTSAC	6 cut(s)	196, 466, 1199, 2129, 2342, 3015
NsiI	ATGCAT	1 cut(s)	2590
NspI	RCATGY	3 cut(s)	1877, 2299, 2545
NspV	TTCGAA	1 cut(s)	2478
PaeR7I	CTCGAG	1 cut(s)	557
PaqCI	CACCTGC	1 cut(s)	2956
PciI	ACATGT	1 cut(s)	1873
PciSI	GCTCTTC	1 cut(s)	2606
PcsI	WCGNNNNNNNCGW	2 cut(s)	711, 2805
PctI	GAATGC	2 cut(s)	1680, 2018
PdmI	GAANNNNTTC	6 cut(s)	296, 935, 1019, 1451, 2322, 2865
PflFI	GACNNNGTC	1 cut(s)	2699
PflMI	CCANNNNNTGG	1 cut(s)	2029
PinAI	ACCGGT	2 cut(s)	1625, 2668
PluTI	GGCGCC	1 cut(s)	960
Ppu21I	YACGTR	3 cut(s)	196, 2800, 2809
PpuMI	RGGWCCY	4 cut(s)	305, 745, 1389, 2172
PscI	ACATGT	1 cut(s)	1873
PshAI	GACNNNNGTC	1 cut(s)	1574
Psp124BI	GAGCTC	4 cut(s)	639, 912, 993, 1599
Psp5II	RGGWCCY	4 cut(s)	305, 745, 1389, 2172
Psp6I	CCWGG	4 cut(s)	131, 792, 993, 2971
PspFI	CCCAGC	2 cut(s)	829, 2096
PspGI	CCWGG	4 cut(s)	131, 792, 993, 2971
PspOMI	GGGCCC	1 cut(s)	2894
PspPPI	RGGWCCY	4 cut(s)	305, 745, 1389, 2172
PspXI	VCTCGAGB	1 cut(s)	557
PstI	CTGCAG	2 cut(s)	1005, 2520
PstNI	CAGNNNCTG	3 cut(s)	153, 1000, 3019
PsuI	RGATCY	2 cut(s)	525, 1215
PsyI	GACNNNGTC	1 cut(s)	2699
RsaI	GTAC	5 cut(s)	563, 2108, 2294, 2366, 2977
RsaNI	GTAC	5 cut(s)	562, 2107, 2293, 2365, 2976
RseI	CAYNNNNRTG	1 cut(s)	473
SacI	GAGCTC	4 cut(s)	639, 912, 993, 1599
SacII	CCGCGG	1 cut(s)	54
SalI	GTCGAC	1 cut(s)	776
SapI	GCTCTTC	1 cut(s)	2606
SaqAI	TTAA	2 cut(s)	620, 734
Sau3AI	GATC	6 cut(s)	525, 1215, 1579, 1938, 2427, 3028
SbfI	CCTGCAGG	1 cut(s)	2520
SdaI	CCTGCAGG	1 cut(s)	2520
SduI	GDGCHC	8 cut(s)	639, 912, 993, 1599, 1763, 1888, 1937, 2898
SfaNI	GCATC	2 cut(s)	1041, 2969
SfcI	CTRYAG	2 cut(s)	1001, 2516
SfoI	GGCGCC	1 cut(s)	958
Sfr274I	CTCGAG	1 cut(s)	557
Sfr303I	CCGCGG	1 cut(s)	54
SfuI	TTCGAA	1 cut(s)	2478
SgrBI	CCGCGG	1 cut(s)	54
SinI	GGWCC	7 cut(s)	135, 162, 305, 745, 1389, 1820, 2172
SlaI	CTCGAG	1 cut(s)	557
SmiMI	CAYNNNNRTG	1 cut(s)	473
SmlI	CTYRAG	3 cut(s)	557, 839, 911
SmoI	CTYRAG	3 cut(s)	557, 839, 911
Sse8387I	CCTGCAGG	1 cut(s)	2520
Sse9I	AATT	9 cut(s)	584, 684, 1783, 2220, 2370, 2473, 2550, 2678, 2871
SspDI	GGCGCC	1 cut(s)	956
SspMI	CTAG	2 cut(s)	566, 2763
SstI	GAGCTC	4 cut(s)	639, 912, 993, 1599
StyI	CCWWGG	1 cut(s)	855
TaaI	ACNGT	5 cut(s)	418, 728, 1133, 2135, 2424
TaqII	GACCGA	2 cut(s)	1346, 2894
TasI	AATT	9 cut(s)	584, 684, 1783, 2220, 2370, 2473, 2550, 2678, 2871
TatI	WGTACW	2 cut(s)	2106, 2292
TauI	GCSGC	6 cut(s)	7, 192, 546, 722, 2387, 3025
Tru1I	TTAA	2 cut(s)	620, 734
Tru9I	TTAA	2 cut(s)	620, 734
TscAI	CASTG	8 cut(s)	158, 1030, 1768, 1897, 2140, 2844, 2959, 3024
TseFI	GTSAC	6 cut(s)	196, 466, 1199, 2129, 2342, 3015
TseI	GCWGC	8 cut(s)	766, 1003, 1029, 1489, 1492, 1677, 1955, 2957
Tsp45I	GTSAC	6 cut(s)	196, 466, 1199, 2129, 2342, 3015
TspDTI	ATGAA	4 cut(s)	1516, 1719, 2315, 2550
TspGWI	ACGGA	5 cut(s)	454, 1674, 1708, 2202, 2703
TspRI	CASTG	8 cut(s)	158, 1030, 1768, 1897, 2140, 2844, 2959, 3024
Tth111I	GACNNNGTC	1 cut(s)	2699
Van91I	CCANNNNNTGG	1 cut(s)	2029
VneI	GTGCAC	1 cut(s)	1884
VpaK11BI	GGWCC	7 cut(s)	135, 162, 305, 745, 1389, 1820, 2172
XapI	RAATTY	3 cut(s)	2220, 2678, 2871
XceI	RCATGY	3 cut(s)	1877, 2299, 2545
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2308
XhoI	CTCGAG	1 cut(s)	557
XmiI	GTMKAC	2 cut(s)	777, 878
XmnI	GAANNNNTTC	6 cut(s)	296, 935, 1019, 1451, 2322, 2865
XspI	CTAG	2 cut(s)	566, 2763
Zsp2I	ATGCAT	1 cut(s)	2590

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.