pycom02g15850

leucine-rich repeat receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012855
Physical Location & Seq
Reverse (-)
12862278 .. 12866191
3914 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g15850.1

Sequence Viewer

Length: 3315 bp
ATGATGGTTGTCCATTTGTATCCAAAACTTTCAATCAAATATTTCAAACCCATCTTCCTTTATCTTTCTCTTTTTTTTTTCTTTTTTTTTTTAAATTTATTATTATTATTTTTTAAATTTAAAAATAAGACAATAAAATCACACAATCTCTACTTCTTACGGAAGAAGAGAATAAAGTCTCACTCTCACTCCAGCTACTCTCTACACCCCGCCCCTCCCTCTTTCTCTTCTCTCCTCCACACACCGCCCACCCAAACACCACGAAAAATGCGGCTCCTTTTCTTCCTCCTCCTCCTCCTCCTCCACCACCACTCTCTCTCCGCCATCGCAATGTCCAACTACCGCGCCCTCCTCTCCCTCAAGTCCTCCATAACCTCCGACCCCAACTCCGCCCTTTCCACCTGGACCCCCACCACCAGCCACTGCACTTGGTCCGGCGTCACCTGCGACTCCTCCCGCCGCTACGTCACCTCCCTCGACCTCTCCGGCCTCGACCTCACCGGCACTCTCTCCCCTGACCTCGCCCACCTCCGCTTCCTCGCCAACCTCACCCTCGCTGATAACCAGTTCTCGGGTCCCATCCCGCCTGAGATCTCCGCCCTCTCCGGCCTCCGCCTCCTCAACCTCTCCAACAACGTCTTCAACACCACCTTCCCCCCTCAGCTCTCCAACCTCACCCGCCTCACCGTCCTCGACCTCTACAACAACAACCTCACCGGTGACCTCCCCGTCTCCGTCACCCACATGACCTCCCTCCGCCACTTGCATTTGGGCGGCAACTTCTTCTCCGGCCAGATCCCTCCCGAGTTCGGCCGCTTCCCATTCCTCGAGTACTTAGCCGTCTCCGGCAACGAACTCGGCGGCCCAATACCCCCCGAAATTGGAAACTTAACCACCTTGAAAGAGCTCTACATCGGCTACTTCAACAGCTACGAAGGCGGAATACCGCCCGAAATTGGAAACTTGTCCAATCTCGTCCGCTTCGACGCCGCCAACTGTAACTTAACTGGAGAGATCCCGCCGGAAGTGGGTCGACTGCAAAATGTCGACACCCTGTTTCTCCAGGTCAATGCTCTTTCCGGGTCGTTGACACTCGAGCTGGGTTACCTCAAGAGCTTGAAATCCATGGACTTATCCAACAACGTGTTTACGGGCGAGATTCCGGGTTCCTTTTCGGAGCTCAAGAACTTGACCCTTTTGAATCTCTTCCGAAACAGGCTCCATGGCGCCATTCCCGACTTTATCGGCGACTTGCCGGAGCTCCAGGTGTTGCAGCTCTGGGAGAACAACTTCACCGGCAGCATACCTCAGGGTCTGGGCAAAAATGGGAAGCTCCAAATTCTCGACCTTTCGTCGAACAAGTTGACGGGAACTCTCCCTCCCGATATGTGTTTCGGGAACAATCTCCAGACCCTCATCACTCTGTCAAACTTCCTTTTCGGGCCGATTCCCGAGTCGCTTGGCCGGTGCGATTCGCTGAGTCGGATTCGAATGGGTGACAACTACCTCAACGGGTCGATTCCGAAAGGGCTGCTTAGTTTGCCCATGCTTACACAAGTCGAGTTGCAGGATAATCTCTTAGACGGTTCGTTTCCTGAGACAGATTCAATCTCGGGGAATCTCGGCCAGATTAGTCTCTCCAATAATCGGCTTTCCGGGTCTCTTCCGCCCAGCATCGGCAACTTCTCCGGCGTCCAGAAGCTTCTTCTCGACGGAAACAAGTTCTCGGGTCGAATTCCACCCGAGATTGGGCGATTACAGCAGGTATCCAAGATGGATTTCAAGCACAACAAGTTCATGGGTCCCATAACCCCCGAAATCAGCCATTGCAAGCTCTTGACATTCGTCGATCTCAGCCGAAACGAGCTCTCCGGCGAGATTCCGAAGGAGATTACTGGTATGAGGATACTGAACTACCTCAACCTGTCGAGAAACCATCTTGTGGGCAGCATTCCGTCGTCTATATCCACAATGCAGAGCCTGACATCTGTGGACTTTTCGTATAACAATCTTTCGGGTTTGGTTCCGGGGACCGGTCAGTTCAGTTACTTCAATTACACATCGTTTCTGGGAAACCCCGATCTCTGCGGTCCCTATTTGGTTCCCTGCAAAGACGGGGTTGCCAATGGCACTCACCAACCTCATGTCAAAGGTGCACTCACTGCTTCACTGAAGCTGTTGCTTGTTATTGGGTTGCTTCTGTGCTCGATCATATTCGCTGTTGCAGCGATAATCAAGGCCCGGTCGTTGAAGAAAGCAAGCGATTCCCGAGCTTGGAAACTGACTGCATTCCAACGGTTGGACTTCACGGTTGATGATGTGTTGGATTCGTTGAAGGAGGACAACATTATTGGGAAAGGAGGGGCTGGGATTGTGTACAAAGGAGCTATGCCTAGTGGTGACAATGTGGCTGTCAAAAGACTCCCTGCAATGAGCAGAGGCTCCTCCCACGACCACGGATTCAACGCCGAAATCCAAACTCTTGGTCGAATTCGGCACAGGCACATTGTTCGACTATTGGGTTTCTGTTCGAATCACGAGACAAATCTTTTGGTCTATGAGTACATGCCCAATGGGAGCTTGGGTGAAGTTCTTCATGGTAAGAAAGGGGGTCACTTGCATTGGGACACAAGGTACAAAATCGCCATTGAGGCTGCAAAGGGTCTATGCTATCTTCACCACGACTGCTCGCCTTTGATCGTCCACAGGGACGTGAAGTCGAATAACATCCTTCTCGACTCCAATTTCGAAGCTCATGTTGCTGATTTCGGCCTTGCCAAGTTCCTGCAGGATTCCGGAACATCCGAATGCATGTCCGCAATTGCTGGTTCTTATGGATATATTGCTCCAGAGTATGCTTACACGTTGAAGGTTGATGAGAAGAGCGACGTTTATAGCTTCGGTGTGGTTCTCTTGGAGCTTGTTAGCGGCAGGAAACCGGTTGGTGAATTTGGGGACGGAGTAGACATAGTCCAGTGGGTGCGAAAAATGACAGACTCAAACAAGGAAGGAGTCCTCAAAATCCTTGACCCTAGACTCCCTTCAGTTCCTCTCCATGAGGTTATGCACGTATTTTACGTTGCAATGCTGTGCGTGGAAGAACAAGCAGTGGAGCGTCCAACAATGCGAGAAGTGGTTCAAATTCTAACCGAGCTTCCAAAGGCACCTGGCTCAAAACTGGTAGAGGATTCAGCCATCACAGAGTCCTCCCCTCCCCCTGCCACTACTGCATCAGAGTCTCCAGGTACAACGTCAAAAGACACGAAAGACCAGCCACCACTGCAGTCTCCGCCGCCTGATCTTCTTAGCATATGA

Protein Analysis

1105

Amino Acids

121.33

Weight (kDa)

7.41

Isoelectric Point (pI)

34.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 115 - 150 2e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 172 - 306 3.2e-09 Leucine-rich repeat region
LRR_8 PF13855 203 - 260 1.3e-06 Leucine rich repeat
LRR_4 PF12799 226 - 266 5.4e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 227 - 286 4.8e-06 Leucine rich repeat
LRR_14 PF23598 247 - 357 5.4e-07 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 780 - 1049 7.2e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 780 - 1047 4e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012855)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49670 AT5G65700 AT5G65700
fragaria_vesca FvH4_1g18710
malus_domestica MD02G1194600.v1.1 MD15G1304900.v1.1
prunus_persica Prupe.6G212200_v2.0.a1
pyrus_communis pycom02g15850 pycom15g26730
rosa_chinensis RchiOBHm_Chr2g0109581
rosa_laevigata RLG00000017747
rosa_multiflora Rmu_sc0000332.1_g000045
rosa_roxburghii Rroxscaffold_2G00134130
rosa_rugosa Rorug02G0160800
rosa_samantha Rh2AG213200 Rh2BG223400 Rh2CG215200 Rh2DG218700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 452
AasI GACNNNNNNGTC 1 cut(s) 728
Acc36I ACCTGC 2 cut(s) 452, 1753
AccB1I GGYRCC 2 cut(s) 1226, 3163
AccB7I CCANNNNNTGG 2 cut(s) 1942, 2299
AccI GTMKAC 3 cut(s) 1033, 1047, 2964
AccII CGCG 1 cut(s) 345
AccIII TCCGGA 1 cut(s) 2795
AclWI GGATC 2 cut(s) 790, 1009
AcoI YGGCCR 4 cut(s) 790, 811, 1462, 1624
AcsI RAATTY 7 cut(s) 94, 116, 1338, 1734, 2490, 2948, 3141
AcuI CTGAAG 2 cut(s) 2192, 3027
AcyI GRCGYC 4 cut(s) 438, 987, 1227, 1692
AfaI GTAC 5 cut(s) 833, 2378, 2564, 2636, 3247
AflIII ACRYGT 2 cut(s) 1143, 2862
AgeI ACCGGT 3 cut(s) 716, 2033, 2938
AhdI GACNNNNNGTC 2 cut(s) 1351, 2716
AjiI CACGTC 1 cut(s) 2713
AjnI CCWGG 5 cut(s) 401, 1062, 1263, 3166, 3241
AjuI GAANNNNNNNTTGG 4 cut(s) 623, 655, 1763, 1795
AloI GAACNNNNNNTCC 2 cut(s) 1363, 1395
Alw21I GWGCWC 6 cut(s) 909, 1182, 1263, 1869, 2158, 2207
Alw26I GTCTC 9 cut(s) 183, 736, 847, 1592, 1640, 1665, 2534, 3243, 3291
Alw44I GTGCAC 1 cut(s) 2154
AlwI GGATC 2 cut(s) 790, 1009
AlwNI CAGNNNCTG 3 cut(s) 423, 1315, 1981
Ama87I CYCGRG 9 cut(s) 571, 803, 827, 1094, 1451, 1612, 1726, 1742, 2268
Aor13HI TCCGGA 1 cut(s) 2795
ApaLI GTGCAC 1 cut(s) 2154
ApeKI GCWGC 6 cut(s) 1273, 1299, 1531, 1947, 2225, 2654
ApoI RAATTY 7 cut(s) 94, 116, 1338, 1734, 2490, 2948, 3141
ArsI GACNNNNNNTTYG 6 cut(s) 2470, 2502, 2533, 2565, 2729, 2761
AsiGI ACCGGT 3 cut(s) 716, 2033, 2938
Asp700I GAANNNNTTC 4 cut(s) 1205, 1289, 2592, 3135
AspLEI GCGC 2 cut(s) 347, 1229
AspS9I GGNCC 9 cut(s) 405, 432, 575, 863, 1442, 1802, 2031, 2090, 2239
AsuC2I CCSGG 5 cut(s) 1081, 1164, 1657, 2028, 2242
AsuII TTCGAA 3 cut(s) 1489, 2531, 2748
AvaI CYCGRG 9 cut(s) 571, 803, 827, 1094, 1451, 1612, 1726, 1742, 2268
AvaII GGWCC 6 cut(s) 405, 432, 575, 1802, 2031, 2090
AxyI CCTNAGG 1 cut(s) 1308
BaeGI GKGCMC 1 cut(s) 2158
BanI GGYRCC 2 cut(s) 1226, 3163
BanII GRGCYC 4 cut(s) 909, 1182, 1263, 1869
BarI GAAGNNNNNNTAC 2 cut(s) 927, 959
BauI CACGAG 1 cut(s) 2537
BbsI GAAGAC 1 cut(s) 631
Bbv12I GWGCWC 6 cut(s) 909, 1182, 1263, 1869, 2158, 2207
BbvCI CCTCAGC 1 cut(s) 660
BbvI GCAGC 6 cut(s) 1285, 1311, 1518, 1959, 2237, 2641
BccI CCATC 6 cut(s) 59, 332, 587, 1768, 1944, 3203
BceAI ACGGC 1 cut(s) 824
BcgI CGANNNNNNTGC 6 cut(s) 838, 872, 1513, 1547, 2492, 2526
BciT130I CCWGG 5 cut(s) 403, 1064, 1265, 3168, 3243
BciVI GTATCC 3 cut(s) 30, 1777, 1899
BcnI CCSGG 5 cut(s) 1081, 1164, 1657, 2028, 2242
BcoDI GTCTC 9 cut(s) 183, 736, 847, 1592, 1640, 1665, 2534, 3243, 3291
BfaI CTAG 2 cut(s) 2394, 3033
BfmI CTRYAG 2 cut(s) 2786, 3281
BfoI RGCGCY 1 cut(s) 1230
BfuAI ACCTGC 2 cut(s) 452, 1753
BfuI GTATCC 3 cut(s) 30, 1777, 1899
BglI GCCNNNNNGGC 1 cut(s) 2651
BglII AGATCT 1 cut(s) 591
BmcAI AGTACT 1 cut(s) 833
Bme18I GGWCC 6 cut(s) 405, 432, 575, 1802, 2031, 2090
BmeRI GACNNNNNGTC 2 cut(s) 1351, 2716
BmeT110I CYCGRG 9 cut(s) 571, 803, 827, 1094, 1451, 1612, 1726, 1742, 2268
BmgBI CACGTC 1 cut(s) 2713
BmgT120I GGNCC 9 cut(s) 405, 432, 575, 863, 1442, 1802, 2031, 2090, 2239
BmsI GCATC 2 cut(s) 1683, 3239
BoxI GACNNNNGTC 1 cut(s) 1844
BpiI GAAGAC 1 cut(s) 631
BpmI CTGGAG 7 cut(s) 175, 1029, 1046, 1247, 1391, 2832, 3225
Bpu10I CCTNAGC 1 cut(s) 660
Bpu14I TTCGAA 3 cut(s) 1489, 2531, 2748
BpuEI CTTGAG 3 cut(s) 344, 1094, 1166
BpuMI CCSGG 5 cut(s) 1081, 1164, 1657, 2028, 2242
BsaAI YACGTR 1 cut(s) 3070
BsaHI GRCGYC 4 cut(s) 438, 987, 1227, 1692
BsaI GGTCTC 1 cut(s) 1665
BsaJI CCNNGG 4 cut(s) 1125, 1222, 2027, 2455
BsaWI WCCGGW 4 cut(s) 716, 2033, 2795, 2938
Bse118I RCCGGY 6 cut(s) 500, 716, 1295, 1464, 2033, 2938
Bse1I ACTGG 5 cut(s) 565, 1012, 1900, 2974, 3183
Bse21I CCTNAGG 1 cut(s) 1308
Bse3DI GCAATG 4 cut(s) 336, 1825, 2436, 3090
BseAI TCCGGA 1 cut(s) 2795
BseBI CCWGG 5 cut(s) 403, 1064, 1265, 3168, 3243
BseDI CCNNGG 4 cut(s) 1125, 1222, 2027, 2455
BseGI GGATG 3 cut(s) 579, 2727, 2801
BseMI GCAATG 4 cut(s) 336, 1825, 2436, 3090
BseMII CTCAG 6 cut(s) 579, 674, 1322, 1469, 1587, 1867
BseNI ACTGG 5 cut(s) 565, 1012, 1900, 2974, 3183
BseSI GKGCMC 1 cut(s) 2158
BseX3I CGGCCG 1 cut(s) 811
BseXI GCAGC 6 cut(s) 1285, 1311, 1518, 1959, 2237, 2641
BseYI CCCAGC 3 cut(s) 1099, 1670, 2366
BsgI GTGCAG 1 cut(s) 409
Bsh1236I CGCG 1 cut(s) 345
Bsh1285I CGRYCG 2 cut(s) 814, 2246
BshNI GGYRCC 2 cut(s) 1226, 3163
BshTI ACCGGT 3 cut(s) 716, 2033, 2938
BsiEI CGRYCG 2 cut(s) 814, 2246
BsiHKAI GWGCWC 6 cut(s) 909, 1182, 1263, 1869, 2158, 2207
BsiHKCI CYCGRG 9 cut(s) 571, 803, 827, 1094, 1451, 1612, 1726, 1742, 2268
BslFI GGGAC 7 cut(s) 561, 1788, 2044, 2076, 2639, 2723, 2969
BsmAI GTCTC 9 cut(s) 183, 736, 847, 1592, 1640, 1665, 2534, 3243, 3291
BsmBI CGTCTC 2 cut(s) 736, 847
BsmFI GGGAC 7 cut(s) 561, 1788, 2044, 2076, 2639, 2723, 2969
BsmI GAATGC 3 cut(s) 1950, 2288, 2813
Bso31I GGTCTC 1 cut(s) 1665
BsoBI CYCGRG 9 cut(s) 571, 803, 827, 1094, 1451, 1612, 1726, 1742, 2268
Bsp119I TTCGAA 3 cut(s) 1489, 2531, 2748
Bsp1286I GDGCHC 6 cut(s) 909, 1182, 1263, 1869, 2158, 2207
Bsp13I TCCGGA 1 cut(s) 2795
Bsp1407I TGTACA 1 cut(s) 2376
Bsp143I GATC 8 cut(s) 591, 795, 1014, 1849, 2080, 2208, 2697, 3298
Bsp19I CCATGG 2 cut(s) 1125, 1222
BspCNI CTCAG 6 cut(s) 580, 673, 1321, 1470, 1588, 1866
BspEI TCCGGA 1 cut(s) 2795
BspFNI CGCG 1 cut(s) 345
BspMAI CTGCAG 2 cut(s) 2790, 3285
BspMI ACCTGC 2 cut(s) 452, 1753
BspPI GGATC 2 cut(s) 790, 1009
BspQI GCTCTTC 1 cut(s) 2876
BspT104I TTCGAA 3 cut(s) 1489, 2531, 2748
BspT107I GGYRCC 2 cut(s) 1226, 3163
BspTNI GGTCTC 1 cut(s) 1665
BsrDI GCAATG 4 cut(s) 336, 1825, 2436, 3090
BsrFI RCCGGY 6 cut(s) 500, 716, 1295, 1464, 2033, 2938
BsrGI TGTACA 1 cut(s) 2376
BsrI ACTGG 5 cut(s) 565, 1012, 1900, 2974, 3183
BssAI RCCGGY 6 cut(s) 500, 716, 1295, 1464, 2033, 2938
BssECI CCNNGG 4 cut(s) 1125, 1222, 2027, 2455
BssMI GATC 8 cut(s) 591, 795, 1014, 1849, 2080, 2208, 2697, 3298
BssNI GRCGYC 4 cut(s) 438, 987, 1227, 1692
BssSI CACGAG 1 cut(s) 2537
BssT1I CCWWGG 2 cut(s) 1125, 1222
Bst2BI CACGAG 1 cut(s) 2537
Bst2UI CCWGG 5 cut(s) 403, 1064, 1265, 3168, 3243
Bst4CI ACNGT 5 cut(s) 688, 998, 1586, 2298, 2311
Bst6I CTCTTC 5 cut(s) 161, 232, 1211, 1668, 2876
BstACI GRCGYC 4 cut(s) 438, 987, 1227, 1692
BstAPI GCANNNNNTGC 1 cut(s) 2162
BstAUI TGTACA 1 cut(s) 2376
BstBAI YACGTR 1 cut(s) 3070
BstBI TTCGAA 3 cut(s) 1489, 2531, 2748
BstC8I GCNNGC 3 cut(s) 1832, 2260, 2690
BstDSI CCRYGG 3 cut(s) 1125, 1222, 2455
BstEII GGTNACC 2 cut(s) 719, 1103
BstF5I GGATG 3 cut(s) 579, 2727, 2801
BstFNI CGCG 1 cut(s) 345
BstH2I RGCGCY 1 cut(s) 1230
BstHHI GCGC 2 cut(s) 347, 1229
BstKTI GATC 8 cut(s) 594, 798, 1017, 1852, 2083, 2211, 2700, 3301
BstMAI GTCTC 9 cut(s) 183, 736, 847, 1592, 1640, 1665, 2534, 3243, 3291
BstMBI GATC 8 cut(s) 591, 795, 1014, 1849, 2080, 2208, 2697, 3298
BstMCI CGRYCG 2 cut(s) 814, 2246
BstNI CCWGG 5 cut(s) 403, 1064, 1265, 3168, 3243
BstNSI RCATGY 2 cut(s) 2569, 2815
BstPAI GACNNNNGTC 1 cut(s) 1844
BstPI GGTNACC 2 cut(s) 719, 1103
BstSFI CTRYAG 2 cut(s) 2786, 3281
BstSLI GKGCMC 1 cut(s) 2158
BstUI CGCG 1 cut(s) 345
BstV1I GCAGC 6 cut(s) 1285, 1311, 1518, 1959, 2237, 2641
BstV2I GAAGAC 1 cut(s) 631
BstX2I RGATCY 3 cut(s) 591, 795, 1014
BstXI CCANNNNNNTGG 1 cut(s) 2483
BstYI RGATCY 3 cut(s) 591, 795, 1014
BstZI CGGCCG 1 cut(s) 811
Bsu36I CCTNAGG 1 cut(s) 1308
BsuI GTATCC 3 cut(s) 30, 1777, 1899
BtgI CCRYGG 3 cut(s) 1125, 1222, 2455
BtgZI GCGATG 1 cut(s) 310
BtrI CACGTC 1 cut(s) 2713
BtsCI GGATG 3 cut(s) 579, 2727, 2801
BtsI GCAGTG 4 cut(s) 421, 2160, 3114, 3278
BtsIMutI CAGTG 6 cut(s) 421, 2160, 2168, 2981, 3114, 3278
BveI ACCTGC 2 cut(s) 452, 1753
Cac8I GCNNGC 3 cut(s) 1832, 2260, 2690
CaiI CAGNNNCTG 3 cut(s) 423, 1315, 1981
CfoI GCGC 2 cut(s) 347, 1229
Cfr10I RCCGGY 6 cut(s) 500, 716, 1295, 1464, 2033, 2938
Cfr13I GGNCC 9 cut(s) 405, 432, 575, 863, 1442, 1802, 2031, 2090, 2239
CseI GACGC 4 cut(s) 427, 995, 1681, 3104
Csp6I GTAC 5 cut(s) 832, 2377, 2563, 2635, 3246
CspAI ACCGGT 3 cut(s) 716, 2033, 2938
CspCI CAANNNNNGTGG 2 cut(s) 410, 445
CviQI GTAC 5 cut(s) 832, 2377, 2563, 2635, 3246
DinI GGCGCC 1 cut(s) 1228
DpnI GATC 8 cut(s) 593, 797, 1016, 1851, 2082, 2210, 2699, 3300
DpnII GATC 8 cut(s) 591, 795, 1014, 1849, 2080, 2208, 2697, 3298
DraI TTTAAA 3 cut(s) 93, 115, 121
DrdI GACNNNNNNGTC 1 cut(s) 728
DriI GACNNNNNGTC 2 cut(s) 1351, 2716
DseDI GACNNNNNNGTC 1 cut(s) 728
EaeI YGGCCR 4 cut(s) 790, 811, 1462, 1624
EagI CGGCCG 1 cut(s) 811
Eam1104I CTCTTC 5 cut(s) 161, 232, 1211, 1668, 2876
Eam1105I GACNNNNNGTC 2 cut(s) 1351, 2716
EarI CTCTTC 5 cut(s) 161, 232, 1211, 1668, 2876
EciI GGCGGA 8 cut(s) 310, 379, 586, 602, 746, 954, 1656, 3279
Ecl136II GAGCTC 4 cut(s) 907, 1180, 1261, 1867
EclXI CGGCCG 1 cut(s) 811
Eco130I CCWWGG 2 cut(s) 1125, 1222
Eco24I GRGCYC 4 cut(s) 909, 1182, 1263, 1869
Eco31I GGTCTC 1 cut(s) 1665
Eco47I GGWCC 6 cut(s) 405, 432, 575, 1802, 2031, 2090
Eco52I CGGCCG 1 cut(s) 811
Eco53kI GAGCTC 4 cut(s) 907, 1180, 1261, 1867
Eco57I CTGAAG 2 cut(s) 2192, 3027
Eco81I CCTNAGG 1 cut(s) 1308
Eco88I CYCGRG 9 cut(s) 571, 803, 827, 1094, 1451, 1612, 1726, 1742, 2268
Eco91I GGTNACC 2 cut(s) 719, 1103
EcoICRI GAGCTC 4 cut(s) 907, 1180, 1261, 1867
EcoO109I RGGNCCY 2 cut(s) 575, 1802
EcoO65I GGTNACC 2 cut(s) 719, 1103
EcoRI GAATTC 2 cut(s) 1734, 2490
EcoRII CCWGG 5 cut(s) 401, 1062, 1263, 3166, 3241
EcoT14I CCWWGG 2 cut(s) 1125, 1222
EcoT22I ATGCAT 1 cut(s) 2813
EcoT38I GRGCYC 4 cut(s) 909, 1182, 1263, 1869
EgeI GGCGCC 1 cut(s) 1228
EheI GGCGCC 1 cut(s) 1228
ErhI CCWWGG 2 cut(s) 1125, 1222
Esp3I CGTCTC 2 cut(s) 736, 847
FalI AAGNNNNNCTT 2 cut(s) 1518, 1550
FaqI GGGAC 7 cut(s) 561, 1788, 2044, 2076, 2639, 2723, 2969
FauI CCCGC 5 cut(s) 217, 464, 591, 686, 1026
FauNDI CATATG 1 cut(s) 3311
FblI GTMKAC 3 cut(s) 1033, 1047, 2964
FokI GGATG 3 cut(s) 566, 2714, 2788
FriOI GRGCYC 4 cut(s) 909, 1182, 1263, 1869
FspBI CTAG 2 cut(s) 2394, 3033
GlaI GCGC 2 cut(s) 346, 1228
GsaI CCCAGC 3 cut(s) 1103, 1674, 2370
GsuI CTGGAG 7 cut(s) 175, 1029, 1046, 1247, 1391, 2832, 3225
HaeII RGCGCY 1 cut(s) 1230
HgaI GACGC 4 cut(s) 427, 995, 1681, 3104
HhaI GCGC 2 cut(s) 347, 1229
Hin1I GRCGYC 4 cut(s) 438, 987, 1227, 1692
Hin6I GCGC 2 cut(s) 345, 1227
HinP1I GCGC 2 cut(s) 345, 1227
HincII GTYRAC 4 cut(s) 1034, 1048, 1089, 1365
HindII GTYRAC 4 cut(s) 1034, 1048, 1089, 1365
HindIII AAGCTT 1 cut(s) 1700
Hpy188I TCNGA 8 cut(s) 379, 1177, 1211, 1485, 1524, 1884, 2806, 3235
Hpy99I CGWCG 6 cut(s) 989, 1357, 1715, 1850, 1960, 2891
HpyAV CCTTC 8 cut(s) 661, 929, 1879, 2329, 2741, 2863, 3002, 3051
HpyCH4III ACNGT 5 cut(s) 688, 998, 1586, 2298, 2311
HpyCH4IV ACGT 9 cut(s) 465, 636, 1143, 2712, 2864, 2889, 3069, 3078, 3251
HpySE526I ACGT 9 cut(s) 465, 636, 1143, 2712, 2864, 2889, 3069, 3078, 3251
Hsp92I GRCGYC 4 cut(s) 438, 987, 1227, 1692
HspAI GCGC 2 cut(s) 345, 1227
KasI GGCGCC 1 cut(s) 1226
KflI GGGWCCC 2 cut(s) 575, 1802
Kpn2I TCCGGA 1 cut(s) 2795
Kzo9I GATC 8 cut(s) 591, 795, 1014, 1849, 2080, 2208, 2697, 3298
LguI GCTCTTC 1 cut(s) 2876
Lsp1109I GCAGC 6 cut(s) 1285, 1311, 1518, 1959, 2237, 2641
LweI GCATC 2 cut(s) 1683, 3239
MaeI CTAG 2 cut(s) 2394, 3033
MaeII ACGT 9 cut(s) 465, 636, 1143, 2712, 2864, 2889, 3069, 3078, 3251
MalI GATC 8 cut(s) 593, 797, 1016, 1851, 2082, 2210, 2699, 3300
MboI GATC 8 cut(s) 591, 795, 1014, 1849, 2080, 2208, 2697, 3298
MfeI CAATTG 1 cut(s) 2820
MflI RGATCY 3 cut(s) 591, 795, 1014
MhlI GDGCHC 6 cut(s) 909, 1182, 1263, 1869, 2158, 2207
Mly113I GGCGCC 1 cut(s) 1227
Mph1103I ATGCAT 1 cut(s) 2813
MroI TCCGGA 1 cut(s) 2795
MroXI GAANNNNTTC 4 cut(s) 1205, 1289, 2592, 3135
MseI TTAA 5 cut(s) 92, 114, 120, 890, 1004
MslI CAYNNNNRTG 3 cut(s) 329, 743, 2806
MunI CAATTG 1 cut(s) 2820
Mva1269I GAATGC 3 cut(s) 1950, 2288, 2813
MvaI CCWGG 5 cut(s) 403, 1064, 1265, 3168, 3243
MvnI CGCG 1 cut(s) 345
NarI GGCGCC 1 cut(s) 1227
NciI CCSGG 5 cut(s) 1081, 1164, 1657, 2028, 2242
NcoI CCATGG 2 cut(s) 1125, 1222
NdeI CATATG 1 cut(s) 3311
NdeII GATC 8 cut(s) 591, 795, 1014, 1849, 2080, 2208, 2697, 3298
NmeAIII GCCGAG 2 cut(s) 837, 1602
NmuCI GTSAC 7 cut(s) 439, 466, 719, 736, 1496, 2399, 2612
NsiI ATGCAT 1 cut(s) 2813
NspI RCATGY 2 cut(s) 2569, 2815
NspV TTCGAA 3 cut(s) 1489, 2531, 2748
PaeR7I CTCGAG 2 cut(s) 827, 1094
PaqCI CACCTGC 1 cut(s) 452
PciSI GCTCTTC 1 cut(s) 2876
PcsI WCGNNNNNNNCGW 2 cut(s) 981, 3075
PctI GAATGC 3 cut(s) 1950, 2288, 2813
PdmI GAANNNNTTC 4 cut(s) 1205, 1289, 2592, 3135
PflFI GACNNNGTC 1 cut(s) 2969
PflMI CCANNNNNTGG 2 cut(s) 1942, 2299
PinAI ACCGGT 3 cut(s) 716, 2033, 2938
PluTI GGCGCC 1 cut(s) 1230
Ppu21I YACGTR 1 cut(s) 3070
PpuMI RGGWCCY 2 cut(s) 575, 1802
PshAI GACNNNNGTC 1 cut(s) 1844
Psp124BI GAGCTC 4 cut(s) 909, 1182, 1263, 1869
Psp5II RGGWCCY 2 cut(s) 575, 1802
Psp6I CCWGG 5 cut(s) 401, 1062, 1263, 3166, 3241
PspEI GGTNACC 2 cut(s) 719, 1103
PspFI CCCAGC 3 cut(s) 1099, 1670, 2366
PspGI CCWGG 5 cut(s) 401, 1062, 1263, 3166, 3241
PspPI GGNCC 9 cut(s) 405, 432, 575, 863, 1442, 1802, 2031, 2090, 2239
PspPPI RGGWCCY 2 cut(s) 575, 1802
PspXI VCTCGAGB 2 cut(s) 827, 1094
PstI CTGCAG 2 cut(s) 2790, 3285
PstNI CAGNNNCTG 3 cut(s) 423, 1315, 1981
PsuI RGATCY 3 cut(s) 591, 795, 1014
PsyI GACNNNGTC 1 cut(s) 2969
RsaI GTAC 5 cut(s) 833, 2378, 2564, 2636, 3247
RsaNI GTAC 5 cut(s) 832, 2377, 2563, 2635, 3246
RseI CAYNNNNRTG 3 cut(s) 329, 743, 2806
SacI GAGCTC 4 cut(s) 909, 1182, 1263, 1869
SalI GTCGAC 2 cut(s) 1032, 1046
SapI GCTCTTC 1 cut(s) 2876
SaqAI TTAA 5 cut(s) 92, 114, 120, 890, 1004
Sau3AI GATC 8 cut(s) 591, 795, 1014, 1849, 2080, 2208, 2697, 3298
Sau96I GGNCC 9 cut(s) 405, 432, 575, 863, 1442, 1802, 2031, 2090, 2239
SbfI CCTGCAGG 1 cut(s) 2790
ScaI AGTACT 1 cut(s) 833
SdaI CCTGCAGG 1 cut(s) 2790
SduI GDGCHC 6 cut(s) 909, 1182, 1263, 1869, 2158, 2207
SfaNI GCATC 2 cut(s) 1683, 3239
SfcI CTRYAG 2 cut(s) 2786, 3281
SfoI GGCGCC 1 cut(s) 1228
Sfr274I CTCGAG 2 cut(s) 827, 1094
SfuI TTCGAA 3 cut(s) 1489, 2531, 2748
SgrAI CRCCGGYG 1 cut(s) 716
SinI GGWCC 6 cut(s) 405, 432, 575, 1802, 2031, 2090
SlaI CTCGAG 2 cut(s) 827, 1094
SmiMI CAYNNNNRTG 3 cut(s) 329, 743, 2806
SmlI CTYRAG 5 cut(s) 359, 827, 1094, 1109, 1181
SmoI CTYRAG 5 cut(s) 359, 827, 1094, 1109, 1181
Sse8387I CCTGCAGG 1 cut(s) 2790
SspDI GGCGCC 1 cut(s) 1226
SspI AATATT 1 cut(s) 41
SspMI CTAG 2 cut(s) 2394, 3033
SstI GAGCTC 4 cut(s) 909, 1182, 1263, 1869
StyI CCWWGG 2 cut(s) 1125, 1222
TaaI ACNGT 5 cut(s) 688, 998, 1586, 2298, 2311
TaiI ACGT 9 cut(s) 468, 639, 1146, 2715, 2867, 2892, 3072, 3081, 3254
TatI WGTACW 3 cut(s) 831, 2376, 2562
TauI GCSGC 8 cut(s) 274, 462, 777, 816, 864, 992, 2931, 3295
Tru1I TTAA 5 cut(s) 92, 114, 120, 890, 1004
Tru9I TTAA 5 cut(s) 92, 114, 120, 890, 1004
TscAI CASTG 6 cut(s) 428, 2167, 2175, 2981, 3114, 3285
TseFI GTSAC 7 cut(s) 439, 466, 719, 736, 1496, 2399, 2612
TseI GCWGC 6 cut(s) 1273, 1299, 1531, 1947, 2225, 2654
Tsp45I GTSAC 7 cut(s) 439, 466, 719, 736, 1496, 2399, 2612
TspDTI ATGAA 2 cut(s) 1786, 2585
TspGWI ACGGA 6 cut(s) 175, 724, 1728, 1944, 2472, 2973
TspRI CASTG 6 cut(s) 428, 2167, 2175, 2981, 3114, 3285
Tth111I GACNNNGTC 1 cut(s) 2969
Van91I CCANNNNNTGG 2 cut(s) 1942, 2299
VneI GTGCAC 1 cut(s) 2154
VpaK11BI GGWCC 6 cut(s) 405, 432, 575, 1802, 2031, 2090
XapI RAATTY 7 cut(s) 94, 116, 1338, 1734, 2490, 2948, 3141
XceI RCATGY 2 cut(s) 2569, 2815
XcmI CCANNNNNNNNNTGG 1 cut(s) 2578
XhoI CTCGAG 2 cut(s) 827, 1094
XmiI GTMKAC 3 cut(s) 1033, 1047, 2964
XmnI GAANNNNTTC 4 cut(s) 1205, 1289, 2592, 3135
XspI CTAG 2 cut(s) 2394, 3033
ZrmI AGTACT 1 cut(s) 833
Zsp2I ATGCAT 1 cut(s) 2813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.