Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0010340

Physical Location & Seq

Genomic Coordinates Reverse (-)

20437529 .. 20454889

Gene Structure 17361 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1204700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1317 bp

ATGGATCCGCTTCTTCCCATTGCGGCGCTCTCGATTCTCATCGGCGCTGTCATCGCCCTCGCCTTCTTCGGAAGCTACTTCCGAAAACGGAGCTCCGAAATCCAGTCCATTTCCAAGCCGGAGCTCCAGTCGGACCAAAAAAAGCACCAATCAAAGCCTCACCAGACTAAGAAGTCTCACGCCAGACCACACTCTCACGCCTCTGACAAGGATCAAAACAAGAAACATCATCCGTTGGATGTGAATACTTTGAAAGGCCATGGAGATGCGGTCACTGACCTATGTTTCTCCTCCGACGGGCGGAGTTTGGCAACAGCTTGTGCGGATGGAGTGCTTAGGGTATTCAAGTTGGATGACGCTTCGAGTAAAAGTTTCAAGTTTCTGAGAATTAGTTTGCCTCCCGGAGCTGGTCATCCTGTAGCAGTTTCATTTTCGGATGATGGATTGTCAATAGTTGTGGCTTCTCAAAGTCTGACCGGTTCTTCTTTGTACATGTATGGTGGGGTGGAAAAACCCAAACCTTCTGAGGAAGCTAGGCAGCAACCCAAGCTTCCTTTGCCTGAAGTCAAGTGGGGCCATCACAAAGTTCACGATAAATTAGGTATTCTCACCCTATCTGGGACCACAGCAAGTTATGGCACTGCTGATGGGAGTACAATTGTTGCTTCATGTTCAGAAGGAACTGACATCAAACTTTGGCATGGTACAACCGGCAAGATTTTGGGGCATGTTGACACAAATCAGTTGAAAAACACCATGGCTGCTATATCACCAAATGGGCGTTTCCTTGCTGCAGCAGCGTTTACTGCAGATGTGAAGGTGTGGGAGATCGTCTATTCAAAGGATGGTTCAGTCAAGGAGGTCACAAAAGCCATGCAACTTAAGGGCCATAAGAGTGCAGTGACTTGGTTATGCTTTACTCCGAACTCAGAACAAATGATCACAGCGTCCAAAGATGGTTCAATAAGAATTTGGAATATCAATGTTCGATTTCATATGGATGAGGATCCAAAAACTCTGAAGGTGTTTCCAATTCCACTTGATTTGGGCAGTGCTGCTGTTCACTATGATCGCCTTAGTTTATCTCCAGATGGAAAAATTTTGGCAGCAACCCATGGTTCGACACTACAATGGTTATGCATTGAAACTGGAAAGGTTTTGGACACAGCTGACAAAGCCCATGAAGGTGACATCACAGGCATTTCGTGGGCACCCAAGCCAATTCCAATGGGTGATGAGAAAGTGTTGGTTTTGGCCACATCAAGTGTCGATAAGAAGGTAAAATTCTGGGTTGCTCCATCTGTTCCTGCATCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006950 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0009267 GO:0009605 GO:0009628 GO:0009987 GO:0009991 GO:0010033 GO:0012505 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0030968 GO:0031369 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0034620 GO:0034976 GO:0035966 GO:0035967 GO:0036293 GO:0036294 GO:0042149 GO:0042221 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051219 GO:0051716 GO:0065007 GO:0070482 GO:0070887 GO:0071310 GO:0071453 GO:0071456 GO:0071496

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

439

Amino Acids

47.22

Weight (kDa)

8.94

Isoelectric Point (pI)

31.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	79 - 157	6.2e-06	THOC3 beta-propeller domain
WD40	PF00400	81 - 115	3.4e-08	WD domain, G-beta repeat
EIF3I	PF24805	211 - 331	8.4e-06	EIF3I
Beta-prop_EML_2	PF23414	214 - 329	1.1e-07	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	215 - 328	6.1e-07	G protein beta WD-40 repeat protein
Beta-prop_WDR5	PF25175	218 - 281	5.5e-06	WDR5 beta-propeller domain
WD40_Prp19	PF24814	219 - 405	9.1e-13	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	220 - 329	5.2e-08	TEP-1 second beta-propeller
Beta-prop_WDR3_1st	PF25173	221 - 328	1e-12	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	227 - 325	2.4e-06	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	255 - 328	2.7e-11	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	285 - 406	2e-12	WDHD1 first WD40 domain
WD40	PF00400	292 - 326	6.7e-09	WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd	PF25168	293 - 373	5.7e-06	WDR36/Utp21 second beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0010340)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00090
fragaria_vesca	FvH4_7g11840 FvH4_7g11840 FvH4_7g11840 FvH4_7g11840
malus_domestica	MD02G1204700.v1.1 MD07G1121300.v1.1
prunus_persica	Prupe.2G145000_v2.0.a1
pyrus_communis	pycom02g16680 pycom07g10320
rosa_chinensis	RchiOBHm_Chr1g0350521
rosa_laevigata	RLG00000028479
rosa_multiflora	Rmu_sc0020900.1_g000001 Rmu_ssc0000083.1_g000024
rosa_roxburghii	Rroxscaffold_4G00304790
rosa_rugosa	Rorug01G0211700
rosa_samantha	Rh1AG226400 Rh1BG194800 Rh1CG211200 Rh1DG223000
rosa_wichuraiana	Rw1G019550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	172
AccB1I	GGYRCC	1 cut(s)	1210
AciI	CCGC	5 cut(s)	8, 23, 269, 301, 323
AclWI	GGATC	4 cut(s)	12, 219, 1001, 1014
AcoI	YGGCCR	1 cut(s)	1254
AcsI	RAATTY	3 cut(s)	969, 1098, 1283
AcuI	CTGAAG	2 cut(s)	582, 1040
AfaI	GTAC	3 cut(s)	491, 655, 706
AfiI	CCNNNNNNNGG	4 cut(s)	297, 300, 407, 618
AflII	CTTAAG	1 cut(s)	881
AflIII	ACRYGT	1 cut(s)	492
AgeI	ACCGGT	1 cut(s)	476
AgsI	TTSAA	7 cut(s)	253, 346, 376, 748, 840, 963, 1145
AluBI	AGCT	8 cut(s)	75, 93, 124, 317, 407, 533, 550, 1169
AluI	AGCT	8 cut(s)	75, 93, 124, 317, 407, 533, 550, 1169
Alw21I	GWGCWC	2 cut(s)	95, 126
Alw26I	GTCTC	1 cut(s)	180
AlwI	GGATC	4 cut(s)	12, 219, 1001, 1014
AoxI	GGCC	4 cut(s)	256, 574, 886, 1254
ApeKI	GCWGC	7 cut(s)	538, 761, 791, 794, 797, 1055, 1106
ApoI	RAATTY	3 cut(s)	969, 1098, 1283
AsiGI	ACCGGT	1 cut(s)	476
AspLEI	GCGC	2 cut(s)	28, 47
AspS9I	GGNCC	4 cut(s)	133, 574, 621, 886
AsuC2I	CCSGG	1 cut(s)	402
AsuHPI	GGTGA	5 cut(s)	152, 601, 762, 1199, 1244
AvaII	GGWCC	2 cut(s)	133, 621
BaeGI	GKGCMC	1 cut(s)	1213
BalI	TGGCCA	1 cut(s)	1256
BamHI	GGATCC	2 cut(s)	4, 1006
BanI	GGYRCC	1 cut(s)	1210
BanII	GRGCYC	2 cut(s)	95, 126
Bbv12I	GWGCWC	2 cut(s)	95, 126
BbvI	GCAGC	7 cut(s)	550, 748, 778, 806, 809, 1042, 1118
BccI	CCATC	8 cut(s)	320, 434, 585, 641, 839, 950, 1085, 1306
BclI	TGATCA	1 cut(s)	939
BcnI	CCSGG	1 cut(s)	402
BcoDI	GTCTC	1 cut(s)	180
BfaI	CTAG	1 cut(s)	534
BfmI	CTRYAG	3 cut(s)	417, 792, 807
BfoI	RGCGCY	2 cut(s)	29, 48
BfrI	CTTAAG	1 cut(s)	881
BisI	GCNGC	8 cut(s)	24, 539, 762, 792, 795, 798, 1056, 1107
BlsI	GCNGC	8 cut(s)	25, 540, 763, 793, 796, 799, 1057, 1108
Bme1390I	CCNGG	1 cut(s)	402
Bme18I	GGWCC	2 cut(s)	133, 621
BmgT120I	GGNCC	4 cut(s)	133, 574, 621, 886
BmiI	GGNNCC	5 cut(s)	6, 575, 622, 1008, 1212
BmrFI	CCNGG	1 cut(s)	402
BmsI	GCATC	1 cut(s)	256
BpmI	CTGGAG	2 cut(s)	110, 1071
Bpu10I	CCTNAGC	1 cut(s)	335
BpuMI	CCSGG	1 cut(s)	402
BsaBI	GATNNNNATC	2 cut(s)	38, 1005
BsaJI	CCNNGG	3 cut(s)	259, 756, 1114
BsaWI	WCCGGW	1 cut(s)	476
BsaXI	ACNNNNNCTCC	4 cut(s)	113, 143, 255, 285
Bsc4I	CCNNNNNNNGG	4 cut(s)	297, 300, 407, 618
Bse118I	RCCGGY	2 cut(s)	476, 710
Bse1I	ACTGG	3 cut(s)	103, 127, 1153
Bse3DI	GCAATG	1 cut(s)	18
Bse8I	GATNNNNATC	2 cut(s)	38, 1005
BseDI	CCNNGG	3 cut(s)	259, 756, 1114
BseGI	GGATG	8 cut(s)	229, 244, 331, 358, 412, 442, 850, 1006
BseJI	GATNNNNATC	2 cut(s)	38, 1005
BseLI	CCNNNNNNNGG	4 cut(s)	297, 300, 407, 618
BseMI	GCAATG	1 cut(s)	18
BseMII	CTCAG	3 cut(s)	374, 516, 942
BseNI	ACTGG	3 cut(s)	103, 127, 1153
BseRI	GAGGAG	1 cut(s)	280
BseSI	GKGCMC	1 cut(s)	1213
BseXI	GCAGC	7 cut(s)	550, 748, 778, 806, 809, 1042, 1118
BsgI	GTGCAG	1 cut(s)	918
BshFI	GGCC	4 cut(s)	258, 576, 888, 1256
BshNI	GGYRCC	1 cut(s)	1210
BshTI	ACCGGT	1 cut(s)	476
BsiHKAI	GWGCWC	2 cut(s)	95, 126
BsiSI	CCGG	4 cut(s)	119, 402, 477, 711
BslFI	GGGAC	1 cut(s)	634
BslI	CCNNNNNNNGG	4 cut(s)	297, 300, 407, 618
BsmAI	GTCTC	1 cut(s)	180
BsmFI	GGGAC	1 cut(s)	634
BsnI	GGCC	4 cut(s)	258, 576, 888, 1256
Bsp1286I	GDGCHC	3 cut(s)	95, 126, 1213
Bsp1407I	TGTACA	1 cut(s)	489
Bsp143I	GATC	6 cut(s)	4, 211, 828, 939, 1006, 1069
Bsp19I	CCATGG	3 cut(s)	259, 756, 1114
BspACI	CCGC	5 cut(s)	8, 23, 269, 301, 323
BspANI	GGCC	4 cut(s)	258, 576, 888, 1256
BspCNI	CTCAG	3 cut(s)	375, 517, 941
BspLI	GGNNCC	5 cut(s)	6, 575, 622, 1008, 1212
BspMAI	CTGCAG	2 cut(s)	796, 811
BspPI	GGATC	4 cut(s)	12, 219, 1001, 1014
BspT107I	GGYRCC	1 cut(s)	1210
BspTI	CTTAAG	1 cut(s)	881
BsrDI	GCAATG	1 cut(s)	18
BsrFI	RCCGGY	2 cut(s)	476, 710
BsrGI	TGTACA	1 cut(s)	489
BsrI	ACTGG	3 cut(s)	103, 127, 1153
BssAI	RCCGGY	2 cut(s)	476, 710
BssECI	CCNNGG	3 cut(s)	259, 756, 1114
BssMI	GATC	6 cut(s)	4, 211, 828, 939, 1006, 1069
BssT1I	CCWWGG	3 cut(s)	259, 756, 1114
BstAFI	CTTAAG	1 cut(s)	881
BstAUI	TGTACA	1 cut(s)	489
BstDEI	CTNAG	6 cut(s)	168, 335, 383, 525, 928, 1076
BstDSI	CCRYGG	3 cut(s)	259, 756, 1114
BstF5I	GGATG	8 cut(s)	229, 244, 331, 358, 412, 442, 850, 1006
BstH2I	RGCGCY	2 cut(s)	29, 48
BstHHI	GCGC	2 cut(s)	28, 47
BstKTI	GATC	6 cut(s)	7, 214, 831, 942, 1009, 1072
BstMAI	GTCTC	1 cut(s)	180
BstMBI	GATC	6 cut(s)	4, 211, 828, 939, 1006, 1069
BstMWI	GCNNNNNNNGC	6 cut(s)	53, 547, 556, 797, 806, 1175
BstNSI	RCATGY	2 cut(s)	496, 731
BstSCI	CCNGG	1 cut(s)	400
BstSFI	CTRYAG	3 cut(s)	417, 792, 807
BstSLI	GKGCMC	1 cut(s)	1213
BstV1I	GCAGC	7 cut(s)	550, 748, 778, 806, 809, 1042, 1118
BstX2I	RGATCY	2 cut(s)	4, 1006
BstYI	RGATCY	2 cut(s)	4, 1006
BsuRI	GGCC	4 cut(s)	258, 576, 888, 1256
BtgI	CCRYGG	3 cut(s)	259, 756, 1114
BtgZI	GCGATG	1 cut(s)	37
BtsCI	GGATG	8 cut(s)	229, 244, 331, 358, 412, 442, 850, 1006
BtsI	GCAGTG	3 cut(s)	639, 906, 1057
BtsIMutI	CAGTG	4 cut(s)	273, 639, 906, 1057
CfoI	GCGC	2 cut(s)	28, 47
Cfr10I	RCCGGY	2 cut(s)	476, 710
Cfr13I	GGNCC	4 cut(s)	133, 574, 621, 886
CseI	GACGC	2 cut(s)	365, 936
Csp6I	GTAC	3 cut(s)	490, 654, 705
CspAI	ACCGGT	1 cut(s)	476
CspCI	CAANNNNNGTGG	4 cut(s)	438, 473, 1026, 1061
CviAII	CATG	9 cut(s)	260, 493, 669, 701, 728, 757, 874, 1115, 1181
CviQI	GTAC	3 cut(s)	490, 654, 705
DdeI	CTNAG	6 cut(s)	168, 335, 383, 525, 928, 1076
DpnI	GATC	6 cut(s)	6, 213, 830, 941, 1008, 1071
DpnII	GATC	6 cut(s)	4, 211, 828, 939, 1006, 1069
DrdI	GACNNNNNNGTC	1 cut(s)	172
DseDI	GACNNNNNNGTC	1 cut(s)	172
EaeI	YGGCCR	1 cut(s)	1254
EciI	GGCGGA	1 cut(s)	316
Ecl136II	GAGCTC	2 cut(s)	93, 124
Eco130I	CCWWGG	3 cut(s)	259, 756, 1114
Eco24I	GRGCYC	2 cut(s)	95, 126
Eco47I	GGWCC	2 cut(s)	133, 621
Eco53kI	GAGCTC	2 cut(s)	93, 124
Eco57I	CTGAAG	2 cut(s)	582, 1040
EcoICRI	GAGCTC	2 cut(s)	93, 124
EcoT14I	CCWWGG	3 cut(s)	259, 756, 1114
EcoT22I	ATGCAT	1 cut(s)	1142
EcoT38I	GRGCYC	2 cut(s)	95, 126
ErhI	CCWWGG	3 cut(s)	259, 756, 1114
FaeI	CATG	9 cut(s)	263, 496, 672, 704, 731, 760, 877, 1118, 1184
FaqI	GGGAC	1 cut(s)	634
FatI	CATG	9 cut(s)	259, 492, 668, 700, 727, 756, 873, 1114, 1180
FauNDI	CATATG	1 cut(s)	996
FbaI	TGATCA	1 cut(s)	939
Fnu4HI	GCNGC	8 cut(s)	24, 539, 762, 792, 795, 798, 1056, 1107
FokI	GGATG	8 cut(s)	216, 251, 338, 365, 399, 449, 857, 1013
FriOI	GRGCYC	2 cut(s)	95, 126
Fsp4HI	GCNGC	8 cut(s)	24, 539, 762, 792, 795, 798, 1056, 1107
FspBI	CTAG	1 cut(s)	534
GlaI	GCGC	2 cut(s)	27, 46
GluI	GCNGC	8 cut(s)	24, 539, 762, 792, 795, 798, 1056, 1107
GsuI	CTGGAG	2 cut(s)	110, 1071
HaeII	RGCGCY	2 cut(s)	29, 48
HaeIII	GGCC	4 cut(s)	258, 576, 888, 1256
HapII	CCGG	4 cut(s)	119, 402, 477, 711
HgaI	GACGC	2 cut(s)	365, 936
HhaI	GCGC	2 cut(s)	28, 47
Hin1II	CATG	9 cut(s)	263, 496, 672, 704, 731, 760, 877, 1118, 1184
Hin6I	GCGC	2 cut(s)	26, 45
HinP1I	GCGC	2 cut(s)	26, 45
HincII	GTYRAC	1 cut(s)	733
HindII	GTYRAC	1 cut(s)	733
HindIII	AAGCTT	1 cut(s)	548
HinfI	GANTC	1 cut(s)	34
HpaII	CCGG	4 cut(s)	119, 402, 477, 711
HphI	GGTGA	5 cut(s)	152, 601, 762, 1199, 1244
Hpy166II	GTNNAC	4 cut(s)	589, 733, 804, 1063
Hpy188III	TCNNGA	3 cut(s)	31, 590, 1088
Hpy8I	GTNNAC	4 cut(s)	589, 733, 804, 1063
Hpy99I	CGWCG	1 cut(s)	299
HpyAV	CCTTC	7 cut(s)	73, 531, 671, 811, 1015, 1178, 1270
HpyCH4V	TGCA	6 cut(s)	794, 809, 877, 899, 1140, 1310
HpyF10VI	GCNNNNNNNGC	6 cut(s)	53, 547, 556, 797, 806, 1175
HpyF3I	CTNAG	6 cut(s)	168, 335, 383, 525, 928, 1076
Hsp92II	CATG	9 cut(s)	263, 496, 672, 704, 731, 760, 877, 1118, 1184
HspAI	GCGC	2 cut(s)	26, 45
Ksp22I	TGATCA	1 cut(s)	939
Kzo9I	GATC	6 cut(s)	4, 211, 828, 939, 1006, 1069
LmnI	GCTCC	6 cut(s)	90, 98, 121, 129, 404, 1300
Lsp1109I	GCAGC	7 cut(s)	550, 748, 778, 806, 809, 1042, 1118
LweI	GCATC	1 cut(s)	256
MaeI	CTAG	1 cut(s)	534
MaeIII	GTNAC	4 cut(s)	271, 862, 901, 1187
MalI	GATC	6 cut(s)	6, 213, 830, 941, 1008, 1071
MboI	GATC	6 cut(s)	4, 211, 828, 939, 1006, 1069
MboII	GAAGA	3 cut(s)	5, 58, 474
MfeI	CAATTG	1 cut(s)	657
MflI	RGATCY	2 cut(s)	4, 1006
MhlI	GDGCHC	3 cut(s)	95, 126, 1213
MlsI	TGGCCA	1 cut(s)	1256
MluCI	AATT	8 cut(s)	387, 596, 657, 969, 1032, 1098, 1221, 1283
MluNI	TGGCCA	1 cut(s)	1256
MmeI	TCCRAC	4 cut(s)	111, 216, 318, 330
MnlI	CCTC	8 cut(s)	68, 168, 211, 301, 408, 520, 853, 997
Mox20I	TGGCCA	1 cut(s)	1256
Mph1103I	ATGCAT	1 cut(s)	1142
MscI	TGGCCA	1 cut(s)	1256
MseI	TTAA	1 cut(s)	882
MslI	CAYNNNNRTG	5 cut(s)	264, 894, 999, 1129, 1185
Msp20I	TGGCCA	1 cut(s)	1256
MspA1I	CMGCKG	1 cut(s)	1169
MspCI	CTTAAG	1 cut(s)	881
MspI	CCGG	4 cut(s)	119, 402, 477, 711
MspR9I	CCNGG	1 cut(s)	402
MunI	CAATTG	1 cut(s)	657
MwoI	GCNNNNNNNGC	6 cut(s)	53, 547, 556, 797, 806, 1175
NciI	CCSGG	1 cut(s)	402
NcoI	CCATGG	3 cut(s)	259, 756, 1114
NdeI	CATATG	1 cut(s)	996
NdeII	GATC	6 cut(s)	4, 211, 828, 939, 1006, 1069
NlaIII	CATG	9 cut(s)	263, 496, 672, 704, 731, 760, 877, 1118, 1184
NlaIV	GGNNCC	5 cut(s)	6, 575, 622, 1008, 1212
NmuCI	GTSAC	4 cut(s)	271, 862, 901, 1187
NsiI	ATGCAT	1 cut(s)	1142
NspI	RCATGY	2 cut(s)	496, 731
PciI	ACATGT	1 cut(s)	492
PfeI	GAWTC	1 cut(s)	34
PfoI	TCCNGGA	1 cut(s)	400
PinAI	ACCGGT	1 cut(s)	476
PkrI	GCNGC	8 cut(s)	25, 540, 763, 793, 796, 799, 1057, 1108
PscI	ACATGT	1 cut(s)	492
Psp124BI	GAGCTC	2 cut(s)	95, 126
PspN4I	GGNNCC	5 cut(s)	6, 575, 622, 1008, 1212
PspPI	GGNCC	4 cut(s)	133, 574, 621, 886
PstI	CTGCAG	2 cut(s)	796, 811
PsuI	RGATCY	2 cut(s)	4, 1006
PvuII	CAGCTG	1 cut(s)	1169
RsaI	GTAC	3 cut(s)	491, 655, 706
RsaNI	GTAC	3 cut(s)	490, 654, 705
RseI	CAYNNNNRTG	5 cut(s)	264, 894, 999, 1129, 1185
SacI	GAGCTC	2 cut(s)	95, 126
SaqAI	TTAA	1 cut(s)	882
SatI	GCNGC	8 cut(s)	24, 539, 762, 792, 795, 798, 1056, 1107
Sau3AI	GATC	6 cut(s)	4, 211, 828, 939, 1006, 1069
Sau96I	GGNCC	4 cut(s)	133, 574, 621, 886
ScrFI	CCNGG	1 cut(s)	402
SduI	GDGCHC	3 cut(s)	95, 126, 1213
SfaNI	GCATC	1 cut(s)	256
SfcI	CTRYAG	3 cut(s)	417, 792, 807
SinI	GGWCC	2 cut(s)	133, 621
SmiMI	CAYNNNNRTG	5 cut(s)	264, 894, 999, 1129, 1185
SmlI	CTYRAG	1 cut(s)	881
SmoI	CTYRAG	1 cut(s)	881
Sse9I	AATT	8 cut(s)	387, 596, 657, 969, 1032, 1098, 1221, 1283
SsiI	CCGC	5 cut(s)	8, 23, 269, 301, 323
SspMI	CTAG	1 cut(s)	534
SstI	GAGCTC	2 cut(s)	95, 126
StyD4I	CCNGG	1 cut(s)	400
StyI	CCWWGG	3 cut(s)	259, 756, 1114
TaqI	TCGA	5 cut(s)	32, 362, 988, 1121, 1269
TasI	AATT	8 cut(s)	387, 596, 657, 969, 1032, 1098, 1221, 1283
TatI	WGTACW	2 cut(s)	489, 653
TauI	GCSGC	1 cut(s)	26
TfiI	GAWTC	1 cut(s)	34
Tru1I	TTAA	1 cut(s)	882
Tru9I	TTAA	1 cut(s)	882
TscAI	CASTG	4 cut(s)	280, 646, 906, 1057
TseFI	GTSAC	4 cut(s)	271, 862, 901, 1187
TseI	GCWGC	7 cut(s)	538, 761, 791, 794, 797, 1055, 1106
Tsp45I	GTSAC	4 cut(s)	271, 862, 901, 1187
TspDTI	ATGAA	4 cut(s)	417, 657, 983, 1197
TspGWI	ACGGA	2 cut(s)	103, 222
TspRI	CASTG	4 cut(s)	280, 646, 906, 1057
Vha464I	CTTAAG	1 cut(s)	881
VpaK11BI	GGWCC	2 cut(s)	133, 621
XapI	RAATTY	3 cut(s)	969, 1098, 1283
XceI	RCATGY	2 cut(s)	496, 731
XspI	CTAG	1 cut(s)	534
Zsp2I	ATGCAT	1 cut(s)	1142

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.