Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0010494

Physical Location & Seq

Genomic Coordinates Reverse (-)

25354809 .. 25364785

Gene Structure 9977 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1218200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2631 bp

ATGGGGAGCACAGGTGAGTCTGATCGAAAAAGGCGTCACTTTAGCTCCATATCTCCCATGGCTGCTACTGCTAAGAAGCAGCCCGTTTTGCCCATTTATGACGACAAAAAGCTTGACGTTAAACTCCTGCAATATCAAATTCAGAAGCTATCACAGAACTTAGAAACTCAGAAGCTGGAGCACTCTATTCTTGAGAATAAATTTTCTCAAATGAAGGACAAACAAAAGGCATATGATTCCACATTAACAGTGGTCAATAAGTCTTGGGAAGAGATGGTTAATGACTTGGAATCTTGTTCCATCCGTTTAAGAGAATCGAACAATCTGAAAGATGCTGAAGACATTTCCATCAGGGATGATGGTGCTCCGTCTGCATTGCAGGATGCTTTTCTTGACCGCCTTTCACAAGCAGATGCGACTGAAAGTTCATGTTCATGTAAAAGTTTTAATCAGATGGAAGAAGATAGAGGGACTACCATTGAAAAGACTAAGAATATTGTAGGTAATGTCATAGCTGCAATTGACAACCAGTGGCGTGTGAAGGATGCATTGCGTGATGCACTTTTAAAAGTGCTTCCAGAAGATGGCACAAGTAGGCAGAAGACATCAACTGACTTTAAAAATGAAGTTAAGAACTTGAGATTGGCCTTTATCGATGTGTTTTTCAAGCATAGAATGTTGGCAAGGGAATTGCAGAGCGATTGTGACTTGGAAGCAAAACATAAAGCTGAGCTTAAACGTTTGAAATGGGAATTAGAAACCGCAGCTGATGAATTAAAAGACATTGATCGTCAACTAGACACTATTAAGCCACAGAAAAATGCTGCAAAAGGCACAGCTTTCCCACTTCCAAATATTGGGAACAAGCATGTGGATAGAGTCAGAGATAAGCAAAAAGATCTGCAAGATATGGAGTCTACTCTCAAGAATCTAATGGACCAAGCTTCATCTCGGCTAATGGACATCAAAGGCCTTCATGAAGAAAGGATAAAAATATTACAGAAGTTATCTAGTTTGCAGAACATGCTTAAAATTGTAGAGTGTATTTCTTCTTCCCAAGCTTATCTCTTGGTGAGAGATCAAATAGAAAAGTCAAAGTCAGAAGTCTTCGAGTGCCAGGCGTTATTTGAGAAACTACAGACTGAGAACGATAATCTTTTGTGGAAGGAGAGGGAACTTAATGTCAAGAGTGACATTGCTGATGTTTTTCAAAGATCTTCTGCAGTTGTTGAATCTAGAATTTCTGATTTGGGGATAGAAATACAGAAACAGATTGAGGAAAGAAAATTGATTGAAGCCAAGCTGGAAGAGGCTTCAAGAGAACCAGGAAGAAAAGAAATCATTAAAGAATTTAAATCCTTGGTTTCTTCATTTCCTGAGAAAATGGGTTCCATGCAAGGTCAATTACGTAAATACAAAGAGGCTGCTTCTGACTTTCATTCTTTAAAGGCTGATGTGCAGTCTCTTTCCAGCATCCTTGATAGGAAGGTGAGAGAGTGTGGGACTTTGTCTGCCAGATCCAAAGATCAAGACACTGAGATACAAAAGTTGAATGCTGTGGTCCAAGATTTAAAGGAATGTGACTCAGAGTTAAATCTGATTTTGGAAATGTATAGAAATGAGTTGACTGATTCAAGGGATGTCTTGGAATCTAGAGATTCAGAATGCAGGGCATGGGCTCGTGTTGAAAGTTTGAAGTCCTCTCTTGATGAACGCAACTTGGAATTACGAGTCAAGACAGCAAATGAAGCTGAGGCCATTTCTCAACAAAGGCTAGCTGCTGCTGAGGCTAAAATTGCTGACTTGAGACAGAAATTTGAAGCTTCCAAAAGGAATATTTTGAGACTTTCTGATATTTTGAAATCCAAAAATGAAGAAAATGAGGCATACTTATCCGAAATTGAGACTATTGGACAGTCATATGATGATAGGCAAACTCAAAACCAACATCTACTGCAGCAAATTACTGAGAGAGATGACTATAACATTAAGCTTGTTTTAGAGGGGGTAAGAGCAAGACAACAGCAGAATGCTGTACTCATGGACAAACGCAAAATGGAGAGGGAGATTCAACTAGGCAATCATTCTCTTAATTTCTACAACATGAAAGCAGCTCGAATTGAAGAACAGTTGAAAATATGCAAAAATCATATTCAGAAAGTAGCAGAAGATAAGTTGCAAAAACAAATTACGTTGGAAAATACTCGAAAGAGAGTGTTAGAAGTCCGAAGGCAATCTCAGCAGTTATGGGAGGCGTTAGACGCATCACAATCTAAAGTTGAGAAAAGTCACAATGGTCTGTCAGATCTGCAGTTGGAACTTGAGAGGGAAAGGTTTGAAAAGAGAAGAAAAGAGGAGGAATTGGAAGTTTTAAAAAGGAAGGCTTCAAGGCTTCGAGCACAGACAGAGGGTTCATCAATTGTTGAAAGGCTTCAACAGGAACTTGGAGAATACAAGGAGATACTGAAGTGTGATGTCTGCCTTGACAGGACAAAACAGGTTGTCATTACAAAATGCTATCACTTGTTCTGCAACCCTTGTGTGAAAAAAGTACTTGAAACTCGACAGAGGAAGTGCCCGAGGTGTTTCATGAGCTTTGGGCCTAATGATGTAAAACCCGTATACATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0006325 GO:0006464 GO:0006513 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009791 GO:0009814 GO:0009817 GO:0009965 GO:0009987 GO:0010016 GO:0010154 GO:0010162 GO:0010228 GO:0010389 GO:0010390 GO:0010431 GO:0010564 GO:0016043 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016740 GO:0019538 GO:0019787 GO:0021700 GO:0022414 GO:0022611 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033523 GO:0036211 GO:0042802 GO:0042803 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0046983 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048608 GO:0048609 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051276 GO:0051301 GO:0051302 GO:0051704 GO:0051707 GO:0051726 GO:0051781 GO:0061458 GO:0065007 GO:0070647 GO:0071695 GO:0071704 GO:0071840 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901987 GO:1901990 GO:1902749 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4 zf-C3HC4_2 zf-C3HC4_3

Protein Analysis

877

Amino Acids

101.35

Weight (kDa)

7.94

Isoelectric Point (pI)

55.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	822 - 862	8.4e-07	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	823 - 862	5.8e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	824 - 862	6.1e-10	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010494)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G44950 AT2G44950
fragaria_vesca	FvH4_7g09510 FvH4_7g09510 FvH4_7g09510
malus_domestica	MD02G1218200.v1.1 MD07G1098900.v1.1
prunus_persica	Prupe.2G122900_v2.0.a1
pyrus_communis	pycom07g08140 pycom12652g00010
rosa_chinensis	RchiOBHm_Chr1g0346311
rosa_laevigata	RLG00000028806
rosa_multiflora	Rmu_co8504721.1_g000001 Rmu_sc0003363.1_g000041
rosa_roxburghii	Rroxscaffold_4G00308720
rosa_rugosa	Rorug01G0179100
rosa_samantha	Rh1AG198500 Rh1BG164500 Rh1CG183800 Rh1DG195200
rosa_wichuraiana	Rw1G016680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	584, 857
AccI	GTMKAC	2 cut(s)	917, 2622
AciI	CCGC	2 cut(s)	397, 762
AclI	AACGTT	1 cut(s)	739
AclWI	GGATC	1 cut(s)	1512
AcsI	RAATTY	5 cut(s)	138, 200, 1239, 1349, 1814
AcuI	CTGAAG	2 cut(s)	357, 2486
AcyI	GRCGYC	1 cut(s)	34
AfaI	GTAC	2 cut(s)	2037, 2553
AfiI	CCNNNNNNNGG	2 cut(s)	584, 857
AjnI	CCWGG	2 cut(s)	1116, 1324
AjuI	GAANNNNNNNTTGG	2 cut(s)	626, 658
Alw21I	GWGCWC	4 cut(s)	11, 183, 367, 2401
Alw26I	GTCTC	4 cut(s)	1467, 1801, 1837, 1898
AlwI	GGATC	1 cut(s)	1512
AlwNI	CAGNNNCTG	1 cut(s)	175
Ama87I	CYCGRG	1 cut(s)	2578
AoxI	GGCC	4 cut(s)	645, 970, 1755, 2600
ApoI	RAATTY	5 cut(s)	138, 200, 1239, 1349, 1814
ArsI	GACNNNNNNTTYG	2 cut(s)	19, 51
Asp700I	GAANNNNTTC	1 cut(s)	2430
AspS9I	GGNCC	3 cut(s)	937, 1561, 2600
AsuHPI	GGTGA	3 cut(s)	26, 1084, 1501
AsuNHI	GCTAGC	1 cut(s)	1774
AvaI	CYCGRG	1 cut(s)	2578
AvaII	GGWCC	2 cut(s)	937, 1561
BaeGI	GKGCMC	1 cut(s)	2579
BaeI	ACNNNNGTAYC	4 cut(s)	1532, 1565, 2453, 2486
BanII	GRGCYC	1 cut(s)	1681
BarI	GAAGNNNNNNTAC	2 cut(s)	1036, 1068
BauI	CACGAG	1 cut(s)	1680
BbsI	GAAGAC	3 cut(s)	345, 608, 1099
Bbv12I	GWGCWC	4 cut(s)	11, 183, 367, 2401
BbvCI	CCTCAGC	2 cut(s)	1752, 1785
BccI	CCATC	6 cut(s)	268, 308, 353, 356, 448, 578
BciT130I	CCWGG	2 cut(s)	1118, 1326
BcoDI	GTCTC	4 cut(s)	1467, 1801, 1837, 1898
BfaI	CTAG	6 cut(s)	797, 1011, 1236, 1653, 1775, 2075
BfmI	CTRYAG	4 cut(s)	1136, 1221, 1955, 2309
BglII	AGATCT	3 cut(s)	898, 1214, 2305
BlpI	GCTNAGC	1 cut(s)	729
BmcAI	AGTACT	1 cut(s)	2553
Bme1390I	CCNGG	2 cut(s)	1118, 1326
Bme18I	GGWCC	2 cut(s)	937, 1561
BmeT110I	CYCGRG	1 cut(s)	2578
BmgT120I	GGNCC	3 cut(s)	937, 1561, 2600
BmiI	GGNNCC	1 cut(s)	1390
BmrFI	CCNGG	2 cut(s)	1118, 1326
BmsI	GCATC	7 cut(s)	322, 373, 403, 535, 547, 1482, 2273
BmtI	GCTAGC	1 cut(s)	1778
BpiI	GAAGAC	3 cut(s)	345, 608, 1099
BpmI	CTGGAG	1 cut(s)	197
Bpu10I	CCTNAGC	2 cut(s)	1752, 1785
Bpu1102I	GCTNAGC	1 cut(s)	729
BpuEI	CTTGAG	5 cut(s)	212, 658, 908, 1825, 2342
Bsa29I	ATCGAT	1 cut(s)	654
BsaAI	YACGTR	1 cut(s)	1409
BsaHI	GRCGYC	1 cut(s)	34
BsaJI	CCNNGG	3 cut(s)	57, 1359, 2579
BsaXI	ACNNNNNCTCC	3 cut(s)	28, 29, 59
Bsc4I	CCNNNNNNNGG	2 cut(s)	584, 857
Bse1I	ACTGG	1 cut(s)	529
Bse3DI	GCAATG	3 cut(s)	374, 548, 1194
BseBI	CCWGG	2 cut(s)	1118, 1326
BseCI	ATCGAT	1 cut(s)	654
BseDI	CCNNGG	3 cut(s)	57, 1359, 2579
BseGI	GGATG	6 cut(s)	300, 361, 388, 550, 1473, 1645
BseLI	CCNNNNNNNGG	2 cut(s)	584, 857
BseMI	GCAATG	3 cut(s)	374, 548, 1194
BseNI	ACTGG	1 cut(s)	529
BseRI	GAGGAG	1 cut(s)	2369
BseSI	GKGCMC	1 cut(s)	2579
BsgI	GTGCAG	1 cut(s)	1478
BshFI	GGCC	4 cut(s)	647, 972, 1757, 2602
BshVI	ATCGAT	1 cut(s)	654
BsiHKAI	GWGCWC	4 cut(s)	11, 183, 367, 2401
BsiHKCI	CYCGRG	1 cut(s)	2578
BslFI	GGGAC	2 cut(s)	484, 1516
BslI	CCNNNNNNNGG	2 cut(s)	584, 857
BsmAI	GTCTC	4 cut(s)	1467, 1801, 1837, 1898
BsmFI	GGGAC	2 cut(s)	484, 1516
BsmI	GAATGC	3 cut(s)	1558, 1670, 2035
BsnI	GGCC	4 cut(s)	647, 972, 1757, 2602
BsoBI	CYCGRG	1 cut(s)	2578
Bsp1286I	GDGCHC	6 cut(s)	11, 183, 367, 1681, 2401, 2579
Bsp143I	GATC	8 cut(s)	22, 787, 898, 1078, 1214, 1517, 1525, 2305
Bsp1720I	GCTNAGC	1 cut(s)	729
Bsp19I	CCATGG	1 cut(s)	57
BspACI	CCGC	2 cut(s)	397, 762
BspANI	GGCC	4 cut(s)	647, 972, 1757, 2602
BspDI	ATCGAT	1 cut(s)	654
BspHI	TCATGA	2 cut(s)	976, 2589
BspLI	GGNNCC	1 cut(s)	1390
BspMAI	CTGCAG	3 cut(s)	1225, 1959, 2313
BspOI	GCTAGC	1 cut(s)	1778
BspPI	GGATC	1 cut(s)	1512
BsrDI	GCAATG	3 cut(s)	374, 548, 1194
BsrI	ACTGG	1 cut(s)	529
BssECI	CCNNGG	3 cut(s)	57, 1359, 2579
BssMI	GATC	8 cut(s)	22, 787, 898, 1078, 1214, 1517, 1525, 2305
BssNAI	GTATAC	1 cut(s)	2623
BssNI	GRCGYC	1 cut(s)	34
BssSI	CACGAG	1 cut(s)	1680
BssT1I	CCWWGG	2 cut(s)	57, 1359
Bst1107I	GTATAC	1 cut(s)	2623
Bst2BI	CACGAG	1 cut(s)	1680
Bst2UI	CCWGG	2 cut(s)	1118, 1326
Bst4CI	ACNGT	3 cut(s)	250, 1917, 2130
Bst6I	CTCTTC	2 cut(s)	264, 1302
BstACI	GRCGYC	1 cut(s)	34
BstAPI	GCANNNNNTGC	1 cut(s)	1024
BstBAI	YACGTR	1 cut(s)	1409
BstC8I	GCNNGC	1 cut(s)	1776
BstDSI	CCRYGG	1 cut(s)	57
BstF5I	GGATG	6 cut(s)	300, 361, 388, 550, 1473, 1645
BstKTI	GATC	8 cut(s)	25, 790, 901, 1081, 1217, 1520, 1528, 2308
BstMAI	GTCTC	4 cut(s)	1467, 1801, 1837, 1898
BstMBI	GATC	8 cut(s)	22, 787, 898, 1078, 1214, 1517, 1525, 2305
BstMWI	GCNNNNNNNGC	9 cut(s)	68, 88, 371, 1024, 1748, 1787, 1796, 2239, 2261
BstNI	CCWGG	2 cut(s)	1118, 1326
BstNSI	RCATGY	2 cut(s)	872, 1027
BstSCI	CCNGG	2 cut(s)	1116, 1324
BstSFI	CTRYAG	4 cut(s)	1136, 1221, 1955, 2309
BstSLI	GKGCMC	1 cut(s)	2579
BstSNI	TACGTA	1 cut(s)	1409
BstV2I	GAAGAC	3 cut(s)	345, 608, 1099
BstX2I	RGATCY	4 cut(s)	898, 1214, 1517, 2305
BstYI	RGATCY	4 cut(s)	898, 1214, 1517, 2305
BstZ17I	GTATAC	1 cut(s)	2623
Bsu15I	ATCGAT	1 cut(s)	654
BsuRI	GGCC	4 cut(s)	647, 972, 1757, 2602
BsuTUI	ATCGAT	1 cut(s)	654
BtgI	CCRYGG	1 cut(s)	57
BtsCI	GGATG	6 cut(s)	300, 361, 388, 550, 1473, 1645
BtsIMutI	CAGTG	3 cut(s)	255, 536, 1533
Cac8I	GCNNGC	1 cut(s)	1776
CaiI	CAGNNNCTG	1 cut(s)	175
CciI	TCATGA	2 cut(s)	976, 2589
Cfr13I	GGNCC	3 cut(s)	937, 1561, 2600
ClaI	ATCGAT	1 cut(s)	654
CseI	GACGC	2 cut(s)	23, 2270
Csp6I	GTAC	2 cut(s)	2036, 2552
CviQI	GTAC	2 cut(s)	2036, 2552
DpnI	GATC	8 cut(s)	24, 789, 900, 1080, 1216, 1519, 1527, 2307
DpnII	GATC	8 cut(s)	22, 787, 898, 1078, 1214, 1517, 1525, 2305
DraI	TTTAAA	6 cut(s)	567, 619, 1354, 1446, 1572, 2373
Eam1104I	CTCTTC	2 cut(s)	264, 1302
EarI	CTCTTC	2 cut(s)	264, 1302
Eco105I	TACGTA	1 cut(s)	1409
Eco130I	CCWWGG	2 cut(s)	57, 1359
Eco147I	AGGCCT	1 cut(s)	972
Eco24I	GRGCYC	1 cut(s)	1681
Eco47I	GGWCC	2 cut(s)	937, 1561
Eco57I	CTGAAG	2 cut(s)	357, 2486
Eco88I	CYCGRG	1 cut(s)	2578
EcoRII	CCWGG	2 cut(s)	1116, 1324
EcoT14I	CCWWGG	2 cut(s)	57, 1359
EcoT22I	ATGCAT	1 cut(s)	550
EcoT38I	GRGCYC	1 cut(s)	1681
ErhI	CCWWGG	2 cut(s)	57, 1359
FalI	AAGNNNNNCTT	4 cut(s)	717, 749, 2368, 2400
FaqI	GGGAC	2 cut(s)	484, 1516
FauNDI	CATATG	2 cut(s)	232, 1921
FblI	GTMKAC	2 cut(s)	917, 2622
FokI	GGATG	6 cut(s)	287, 368, 395, 557, 1460, 1652
FriOI	GRGCYC	1 cut(s)	1681
FspBI	CTAG	6 cut(s)	797, 1011, 1236, 1653, 1775, 2075
GsuI	CTGGAG	1 cut(s)	197
HaeIII	GGCC	4 cut(s)	647, 972, 1757, 2602
HgaI	GACGC	2 cut(s)	23, 2270
Hin1I	GRCGYC	1 cut(s)	34
HincII	GTYRAC	2 cut(s)	794, 1626
HindII	GTYRAC	2 cut(s)	794, 1626
HindIII	AAGCTT	5 cut(s)	110, 942, 1059, 1821, 1991
HphI	GGTGA	3 cut(s)	26, 1084, 1501
Hpy166II	GTNNAC	4 cut(s)	794, 918, 1626, 2623
Hpy8I	GTNNAC	4 cut(s)	794, 918, 1626, 2623
HpyAV	CCTTC	7 cut(s)	208, 535, 983, 1159, 1480, 2223, 2374
HpyCH4III	ACNGT	3 cut(s)	250, 1917, 2130
HpyCH4IV	ACGT	4 cut(s)	117, 739, 1408, 2192
HpyF10VI	GCNNNNNNNGC	9 cut(s)	68, 88, 371, 1024, 1748, 1787, 1796, 2239, 2261
HpySE526I	ACGT	4 cut(s)	117, 739, 1408, 2192
Hsp92I	GRCGYC	1 cut(s)	34
Kzo9I	GATC	8 cut(s)	22, 787, 898, 1078, 1214, 1517, 1525, 2305
LmnI	GCTCC	4 cut(s)	6, 50, 178, 370
LweI	GCATC	7 cut(s)	322, 373, 403, 535, 547, 1482, 2273
MaeI	CTAG	6 cut(s)	797, 1011, 1236, 1653, 1775, 2075
MaeII	ACGT	4 cut(s)	117, 739, 1408, 2192
MaeIII	GTNAC	5 cut(s)	35, 704, 1190, 1580, 2288
MalI	GATC	8 cut(s)	24, 789, 900, 1080, 1216, 1519, 1527, 2307
MboI	GATC	8 cut(s)	22, 787, 898, 1078, 1214, 1517, 1525, 2305
MfeI	CAATTG	2 cut(s)	519, 2418
MflI	RGATCY	4 cut(s)	898, 1214, 1517, 2305
MhlI	GDGCHC	6 cut(s)	11, 183, 367, 1681, 2401, 2579
MlyI	GAGTC	5 cut(s)	26, 888, 923, 1577, 1740
MmeI	TCCRAC	2 cut(s)	2175, 2295
Mph1103I	ATGCAT	1 cut(s)	550
MroXI	GAANNNNTTC	1 cut(s)	2430
MslI	CAYNNNNRTG	1 cut(s)	433
MspA1I	CMGCKG	1 cut(s)	767
MspR9I	CCNGG	2 cut(s)	1118, 1326
MunI	CAATTG	2 cut(s)	519, 2418
Mva1269I	GAATGC	3 cut(s)	1558, 1670, 2035
MvaI	CCWGG	2 cut(s)	1118, 1326
MwoI	GCNNNNNNNGC	9 cut(s)	68, 88, 371, 1024, 1748, 1787, 1796, 2239, 2261
NcoI	CCATGG	1 cut(s)	57
NdeI	CATATG	2 cut(s)	232, 1921
NdeII	GATC	8 cut(s)	22, 787, 898, 1078, 1214, 1517, 1525, 2305
NheI	GCTAGC	1 cut(s)	1774
NlaIV	GGNNCC	1 cut(s)	1390
NmeAIII	GCCGAG	1 cut(s)	931
NmuCI	GTSAC	5 cut(s)	35, 704, 1190, 1580, 2288
NsiI	ATGCAT	1 cut(s)	550
NspI	RCATGY	2 cut(s)	872, 1027
PagI	TCATGA	2 cut(s)	976, 2589
PceI	AGGCCT	1 cut(s)	972
PcsI	WCGNNNNNNNCGW	1 cut(s)	31
PctI	GAATGC	3 cut(s)	1558, 1670, 2035
PdmI	GAANNNNTTC	1 cut(s)	2430
PfeI	GAWTC	9 cut(s)	236, 290, 314, 928, 1232, 1631, 1649, 1658, 2068
PflFI	GACNNNGTC	1 cut(s)	1507
PflMI	CCANNNNNTGG	2 cut(s)	584, 857
PleI	GAGTC	5 cut(s)	25, 887, 922, 1577, 1739
PpsI	GAGTC	5 cut(s)	25, 887, 922, 1577, 1739
Ppu21I	YACGTR	1 cut(s)	1409
Psp1406I	AACGTT	1 cut(s)	739
Psp6I	CCWGG	2 cut(s)	1116, 1324
PspGI	CCWGG	2 cut(s)	1116, 1324
PspN4I	GGNNCC	1 cut(s)	1390
PspPI	GGNCC	3 cut(s)	937, 1561, 2600
PstI	CTGCAG	3 cut(s)	1225, 1959, 2313
PstNI	CAGNNNCTG	1 cut(s)	175
PsuI	RGATCY	4 cut(s)	898, 1214, 1517, 2305
PsyI	GACNNNGTC	1 cut(s)	1507
PvuII	CAGCTG	1 cut(s)	767
RsaI	GTAC	2 cut(s)	2037, 2553
RsaNI	GTAC	2 cut(s)	2036, 2552
RseI	CAYNNNNRTG	1 cut(s)	433
Sau3AI	GATC	8 cut(s)	22, 787, 898, 1078, 1214, 1517, 1525, 2305
Sau96I	GGNCC	3 cut(s)	937, 1561, 2600
ScaI	AGTACT	1 cut(s)	2553
SchI	GAGTC	5 cut(s)	26, 888, 923, 1577, 1740
ScrFI	CCNGG	2 cut(s)	1118, 1326
SduI	GDGCHC	6 cut(s)	11, 183, 367, 1681, 2401, 2579
SfaNI	GCATC	7 cut(s)	322, 373, 403, 535, 547, 1482, 2273
SfcI	CTRYAG	4 cut(s)	1136, 1221, 1955, 2309
SinI	GGWCC	2 cut(s)	937, 1561
SmiI	ATTTAAAT	1 cut(s)	1354
SmiMI	CAYNNNNRTG	1 cut(s)	433
SmlI	CTYRAG	5 cut(s)	191, 637, 923, 1804, 2321
SmoI	CTYRAG	5 cut(s)	191, 637, 923, 1804, 2321
SnaBI	TACGTA	1 cut(s)	1409
SseBI	AGGCCT	1 cut(s)	972
SsiI	CCGC	2 cut(s)	397, 762
SspI	AATATT	4 cut(s)	496, 856, 996, 1837
SspMI	CTAG	6 cut(s)	797, 1011, 1236, 1653, 1775, 2075
StuI	AGGCCT	1 cut(s)	972
StyD4I	CCNGG	2 cut(s)	1116, 1324
StyI	CCWWGG	2 cut(s)	57, 1359
SwaI	ATTTAAAT	1 cut(s)	1354
TaaI	ACNGT	3 cut(s)	250, 1917, 2130
TaiI	ACGT	4 cut(s)	120, 742, 1411, 2195
TaqI	TCGA	8 cut(s)	25, 317, 654, 1110, 2116, 2206, 2395, 2563
TatI	WGTACW	2 cut(s)	2035, 2551
TfiI	GAWTC	9 cut(s)	236, 290, 314, 928, 1232, 1631, 1649, 1658, 2068
TscAI	CASTG	3 cut(s)	255, 536, 1540
TseFI	GTSAC	5 cut(s)	35, 704, 1190, 1580, 2288
Tsp45I	GTSAC	5 cut(s)	35, 704, 1190, 1580, 2288
TspGWI	ACGGA	2 cut(s)	293, 357
TspRI	CASTG	3 cut(s)	255, 536, 1540
Tth111I	GACNNNGTC	1 cut(s)	1507
Van91I	CCANNNNNTGG	2 cut(s)	584, 857
VpaK11BI	GGWCC	2 cut(s)	937, 1561
XapI	RAATTY	5 cut(s)	138, 200, 1239, 1349, 1814
XbaI	TCTAGA	2 cut(s)	1235, 1652
XceI	RCATGY	2 cut(s)	872, 1027
XcmI	CCANNNNNNNNNTGG	2 cut(s)	55, 247
XmiI	GTMKAC	2 cut(s)	917, 2622
XmnI	GAANNNNTTC	1 cut(s)	2430
XspI	CTAG	6 cut(s)	797, 1011, 1236, 1653, 1775, 2075
ZrmI	AGTACT	1 cut(s)	2553
Zsp2I	ATGCAT	1 cut(s)	550

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.