Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0010494

Physical Location & Seq

Genomic Coordinates Forward (+)

10893703 .. 10905106

Gene Structure 11404 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1098900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2622 bp

ATGGGGAGCACAGGTGAGTCTGATCGAAAACGGCGTCACTTTAACTCCTTATCACCCATGGCTGCTACTGCTAAGAAGCAGCCCATTTTGCCCATTTCCGAGGACAAAAAGCTTAACATTACTGTCATGCAAGTTCAAAATCAAAATCTATCACGGAAGTTAGAGACTCAGAAGATGGAGCACTCTATTCTTGAGAATAAATTTTCTCAAATGAAGGACAAGCAAAAGGCATATGATTCCATATTAACGGTGGTCAATAAGTCTTGGGAAGAGATGGTTAATGACTTGGAATCTTGTTCCATCCGTACAAGAGAATCGAACAGTCAGAAAGATGCTAAAGACATTTCCATCATGGAAGATGGCGCTCCATCTGCATTGCAGGATGCTTTTCTTAACCGTCTTGCACAAGCAGATGCAACTGAAAGTTCATGTACATATAAAAGTTCTAATCAGATGGAAGAAGATAAAGGTGCTCCCTGTGAAAAGACTAAGAATATTGTATGTAATGTTGTAGCTGCAATTGACAACCAGTGGCGTGTGAAGGATGCATTGTATGATGCACTTGTGAAAGAGCTTCCAGAAGATGGCACAAGTAGGCAGAAGACATCAACTGACTTTAAAAATGAAGTTAAGAACTTGAGATTGGCCTTTATGGATATGTTTTTGAAGCACAGAACATTGGCAAGGGAACTGCAGAGCCACCGTGACTTGGATGCAAAGAATAAAGCTGAGCTTAAACGTTTGAAAGGGGAGTTAAAAATTGTAGTTGGTGAATTAGAAGACAGTGATCGTCAACTAAAACAGAAAGTTACTACCGCAAAAGGCACAGTTTCCCCAGTTCTAAATGTTGGGAACAAGCATGTTGATAGGGTAAGAGATAAGCAAAAAGATCTGCAAGATATGGAGTCTACTCTCAAGGATCTAATGGACCAAGCTTCATCTCGACTAATGGACATCAAAGGCCTTCATGAAGAAAGGCTAAAAATATTACAGCAGTTATCTAGTTTGCAGAACATGCTTAAGAATGTGATATGTATTTCTTCTTCCCAAGCCTTTCTCTTGGTGAGAGATCAAATAGAAAAATCAAAGTCAGAAGTTTTTGAGTGCCAGGCCTTATTTGAGAAACTACAGGCTGAGAAGGATAATCTTGTGTGGAGGGAGAGGGAGCTTAATGTCAAGAGTGACATTGCTGATGTCTTTCGAAGATCTTCTGCAGTTGTTGAATCTAGAATTTCTGATCTGGGTACAGAAATACAGAAACAGATTGAGGAAAGAAAAATGATTGAAGCCAAGCTGGAAGAGGCATCAAGAGAACCAGGAAGAAAAGAAATCATTAAAGAATTTAAAGCTTTGGTTTCATCATTTCCTGAGAAAATGGGCACCATGCAAGATCAATTACGTAAATACAAAGAGGCTGCTTCTGATTTTCATTCTTTGAAGGCTGATGTGCAGTCTCTATCCAGCATCCTTGATAGGAAGGTGAAAGAGTGTGAGACTTTATCTGCCAGATCCAAAGATCAAGATGCTGAGATACAAAAGTTGAATGTTGTGGTCCAAGATTTAAAGGAAAGCGAGTCAGAGTTAAATCTGATTTTGGAAATGTATAGACGTGAATTGACTGATTCAAGGGATGTATTGGAAGCTAGAGATTCAGAATGCAAGGCATGGGCTCATGTTGAAAGTTTGAAGTCCTCTCTTGACGAACGCAACTTGGAATTACGGGTTAAGAGAGCAAATGAAGCTGAGGCTATTTCTCAACAAAGGCTAGCTGCTGCTGAAGCTGAGATTGCTGACTTGAGACAGAAATTTGAAGCTTCCAAAAGGGATATTTTGAGACTTTCTGATGTTCTGAAATCCAAAAATGAAGAAAACGAGGCATACTTATCTGAAATTGAGACAATTGGACAGGCATATGATGATATGCAAACTCAAAACCAACATCTACTGCAGCAAATTACTGAGAGAGATGACTATAACATTAAGCTTGTTTTAGAGGGGGTAAGGGCAAGACAACAGCAGAATGCTGTACTCATGGACAATAGGAAAATGGAGAGGGAGATTCAACAAGGCCATCATTCTCTTAATTTCTATAACATGAAAGCCGCTCGAATCGAAGATCAGTTGAAAATATGCAAGGATCATGTTCAGAAAGTAGCAGAAGATAAGTTGCAAAAACAAATTACGTGGGAAAATACTCAAAAGAGATTGTTAGAAGTTCGAAGGCAATCTCAGCAGTTACGGGAGGCTTTAGCCGCATCACAATCTAAAGTTGGAGAAAGTCGAAAGGGTCTGTCAGAGCTGCAGTTGGAACTAGAGAAGGAACGGTTTGAAAAGAGAAGAAAAGAGGAGGAACTGGAAGTTTTAAAAAGAAAGGCTTCACGGCTTCGAGCAGAGACGGAGGCTTCATCAATTGTTGAAAAGCTTCAACAGGAACTTGGAGAATACAAGGAGATACTGAAATGTGATGTCTGCCTTGACAGGACAAAACAGGTTGTCATTACAAAATGCTATCACTTGTTCTGCAACCCTTGTGTGAAAAAAGTACTTGAAACTCGACAGAGGAAGTGCCCGAGGTGTTCTGTGAGCTTTGGACCTAATGATGTAAAACCTGTATACATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0006325 GO:0006464 GO:0006513 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009791 GO:0009814 GO:0009817 GO:0009965 GO:0009987 GO:0010016 GO:0010154 GO:0010162 GO:0010228 GO:0010389 GO:0010390 GO:0010431 GO:0010564 GO:0016043 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016740 GO:0019538 GO:0019787 GO:0021700 GO:0022414 GO:0022611 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033523 GO:0036211 GO:0042802 GO:0042803 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0046983 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048608 GO:0048609 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051276 GO:0051301 GO:0051302 GO:0051704 GO:0051707 GO:0051726 GO:0051781 GO:0061458 GO:0065007 GO:0070647 GO:0071695 GO:0071704 GO:0071840 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901987 GO:1901990 GO:1902749 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4 zf-C3HC4_2 zf-C3HC4_3

Protein Analysis

874

Amino Acids

100.69

Weight (kDa)

7.96

Isoelectric Point (pI)

54.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	819 - 860	5.2e-07	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	820 - 859	5.8e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	821 - 859	6.1e-10	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010494)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G44950 AT2G44950
fragaria_vesca	FvH4_7g09510 FvH4_7g09510 FvH4_7g09510
malus_domestica	MD02G1218200.v1.1 MD07G1098900.v1.1
prunus_persica	Prupe.2G122900_v2.0.a1
pyrus_communis	pycom07g08140 pycom12652g00010
rosa_chinensis	RchiOBHm_Chr1g0346311
rosa_laevigata	RLG00000028806
rosa_multiflora	Rmu_co8504721.1_g000001 Rmu_sc0003363.1_g000041
rosa_roxburghii	Rroxscaffold_4G00308720
rosa_rugosa	Rorug01G0179100
rosa_samantha	Rh1AG198500 Rh1BG164500 Rh1CG183800 Rh1DG195200
rosa_wichuraiana	Rw1G016680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1379
AccB7I	CCANNNNNTGG	1 cut(s)	584
AccBSI	CCGCTC	1 cut(s)	2105
AccI	GTMKAC	2 cut(s)	908, 2613
AciI	CCGC	3 cut(s)	816, 2103, 2253
AclI	AACGTT	1 cut(s)	739
AclWI	GGATC	3 cut(s)	927, 1503, 2145
AcsI	RAATTY	4 cut(s)	200, 1230, 1340, 1805
AcuI	CTGAAG	1 cut(s)	1797
AcyI	GRCGYC	1 cut(s)	34
AfaI	GTAC	5 cut(s)	307, 433, 1246, 2028, 2544
AfiI	CCNNNNNNNGG	2 cut(s)	584, 709
AflII	CTTAAG	1 cut(s)	1019
AjiI	CACGTC	1 cut(s)	1610
AjnI	CCWGG	2 cut(s)	1107, 1315
AjuI	GAANNNNNNNTTGG	2 cut(s)	626, 658
Alw21I	GWGCWC	3 cut(s)	11, 183, 475
Alw26I	GTCTC	7 cut(s)	158, 1458, 1487, 1792, 1828, 1889, 2387
AlwI	GGATC	3 cut(s)	927, 1503, 2145
Ama87I	CYCGRG	1 cut(s)	2569
AoxI	GGCC	4 cut(s)	645, 961, 1110, 2068
ApeKI	GCWGC	8 cut(s)	62, 79, 515, 1415, 1769, 1772, 1948, 2299
ApoI	RAATTY	4 cut(s)	200, 1230, 1340, 1805
ArsI	GACNNNNNNTTYG	2 cut(s)	19, 51
Asp700I	GAANNNNTTC	3 cut(s)	1207, 2374, 2421
AspLEI	GCGC	1 cut(s)	365
AspS9I	GGNCC	3 cut(s)	928, 1552, 2591
AsuHPI	GGTGA	5 cut(s)	26, 45, 782, 1075, 1492
AsuII	TTCGAA	2 cut(s)	1201, 2218
AsuNHI	GCTAGC	1 cut(s)	1765
AvaI	CYCGRG	1 cut(s)	2569
AvaII	GGWCC	3 cut(s)	928, 1552, 2591
BaeGI	GKGCMC	2 cut(s)	1382, 2570
BaeI	ACNNNNGTAYC	2 cut(s)	1523, 1556
BanI	GGYRCC	1 cut(s)	1379
BanII	GRGCYC	1 cut(s)	1672
BarI	GAAGNNNNNNTAC	2 cut(s)	1027, 1059
BbsI	GAAGAC	2 cut(s)	608, 786
Bbv12I	GWGCWC	3 cut(s)	11, 183, 475
BbvCI	CCTCAGC	1 cut(s)	1743
BbvI	GCAGC	8 cut(s)	49, 91, 502, 1402, 1756, 1759, 1960, 2286
BccI	CCATC	9 cut(s)	169, 268, 308, 353, 356, 376, 448, 578, 2079
BceAI	ACGGC	2 cut(s)	47, 2396
BciT130I	CCWGG	2 cut(s)	1109, 1317
BcoDI	GTCTC	7 cut(s)	158, 1458, 1487, 1792, 1828, 1889, 2387
BfaI	CTAG	5 cut(s)	1002, 1227, 1644, 1766, 2312
BfmI	CTRYAG	5 cut(s)	692, 1127, 1212, 1946, 2300
BfoI	RGCGCY	1 cut(s)	366
BfrI	CTTAAG	1 cut(s)	1019
BglII	AGATCT	2 cut(s)	889, 1205
BlpI	GCTNAGC	1 cut(s)	729
BmcAI	AGTACT	1 cut(s)	2544
Bme1390I	CCNGG	2 cut(s)	1109, 1317
Bme18I	GGWCC	3 cut(s)	928, 1552, 2591
BmeT110I	CYCGRG	1 cut(s)	2569
BmgBI	CACGTC	1 cut(s)	1610
BmgT120I	GGNCC	3 cut(s)	928, 1552, 2591
BmiI	GGNNCC	1 cut(s)	1381
BmrFI	CCNGG	2 cut(s)	1109, 1317
BmrI	ACTGGG	1 cut(s)	830
BmtI	GCTAGC	1 cut(s)	1769
BmuI	ACTGGG	1 cut(s)	830
BpiI	GAAGAC	2 cut(s)	608, 786
Bpu10I	CCTNAGC	1 cut(s)	1743
Bpu1102I	GCTNAGC	1 cut(s)	729
Bpu14I	TTCGAA	2 cut(s)	1201, 2218
BpuEI	CTTGAG	4 cut(s)	212, 658, 899, 1816
BsaAI	YACGTR	2 cut(s)	1400, 2184
BsaHI	GRCGYC	1 cut(s)	34
BsaJI	CCNNGG	3 cut(s)	57, 99, 2570
BsaXI	ACNNNNNCTCC	3 cut(s)	28, 29, 59
Bsc4I	CCNNNNNNNGG	2 cut(s)	584, 709
Bse1I	ACTGG	3 cut(s)	529, 836, 2358
Bse3DI	GCAATG	2 cut(s)	374, 1185
BseBI	CCWGG	2 cut(s)	1109, 1317
BseDI	CCNNGG	3 cut(s)	57, 99, 2570
BseGI	GGATG	6 cut(s)	300, 388, 550, 718, 1464, 1636
BseLI	CCNNNNNNNGG	2 cut(s)	584, 709
BseMI	GCAATG	2 cut(s)	374, 1185
BseMII	CTCAG	9 cut(s)	182, 720, 1125, 1359, 1518, 1734, 1773, 1950, 2243
BseNI	ACTGG	3 cut(s)	529, 836, 2358
BseRI	GAGGAG	1 cut(s)	2360
BseSI	GKGCMC	2 cut(s)	1382, 2570
BseXI	GCAGC	8 cut(s)	49, 91, 502, 1402, 1756, 1759, 1960, 2286
BsgI	GTGCAG	1 cut(s)	1469
BshFI	GGCC	4 cut(s)	647, 963, 1112, 2070
BshNI	GGYRCC	1 cut(s)	1379
BsiHKAI	GWGCWC	3 cut(s)	11, 183, 475
BsiHKCI	CYCGRG	1 cut(s)	2569
BslI	CCNNNNNNNGG	2 cut(s)	584, 709
BsmAI	GTCTC	7 cut(s)	158, 1458, 1487, 1792, 1828, 1889, 2387
BsmBI	CGTCTC	1 cut(s)	2387
BsmI	GAATGC	2 cut(s)	1661, 2026
BsnI	GGCC	4 cut(s)	647, 963, 1112, 2070
BsoBI	CYCGRG	1 cut(s)	2569
Bsp119I	TTCGAA	2 cut(s)	1201, 2218
Bsp1286I	GDGCHC	6 cut(s)	11, 183, 475, 1382, 1672, 2570
Bsp1407I	TGTACA	1 cut(s)	431
Bsp1720I	GCTNAGC	1 cut(s)	729
Bsp19I	CCATGG	1 cut(s)	57
BspACI	CCGC	3 cut(s)	816, 2103, 2253
BspANI	GGCC	4 cut(s)	647, 963, 1112, 2070
BspCNI	CTCAG	9 cut(s)	181, 721, 1126, 1360, 1519, 1735, 1774, 1951, 2242
BspHI	TCATGA	1 cut(s)	967
BspLI	GGNNCC	1 cut(s)	1381
BspMAI	CTGCAG	4 cut(s)	696, 1216, 1950, 2304
BspOI	GCTAGC	1 cut(s)	1769
BspPI	GGATC	3 cut(s)	927, 1503, 2145
BspT104I	TTCGAA	2 cut(s)	1201, 2218
BspT107I	GGYRCC	1 cut(s)	1379
BspTI	CTTAAG	1 cut(s)	1019
BsrBI	CCGCTC	1 cut(s)	2105
BsrDI	GCAATG	2 cut(s)	374, 1185
BsrGI	TGTACA	1 cut(s)	431
BsrI	ACTGG	3 cut(s)	529, 836, 2358
BssECI	CCNNGG	3 cut(s)	57, 99, 2570
BssNAI	GTATAC	1 cut(s)	2614
BssNI	GRCGYC	1 cut(s)	34
BssT1I	CCWWGG	1 cut(s)	57
Bst1107I	GTATAC	1 cut(s)	2614
Bst2UI	CCWGG	2 cut(s)	1109, 1317
Bst4CI	ACNGT	8 cut(s)	124, 250, 323, 398, 704, 785, 829, 2325
Bst6I	CTCTTC	2 cut(s)	264, 1293
BstACI	GRCGYC	1 cut(s)	34
BstAFI	CTTAAG	1 cut(s)	1019
BstAPI	GCANNNNNTGC	1 cut(s)	1015
BstAUI	TGTACA	1 cut(s)	431
BstBAI	YACGTR	2 cut(s)	1400, 2184
BstBI	TTCGAA	2 cut(s)	1201, 2218
BstC8I	GCNNGC	1 cut(s)	1767
BstDSI	CCRYGG	1 cut(s)	57
BstF5I	GGATG	6 cut(s)	300, 388, 550, 718, 1464, 1636
BstH2I	RGCGCY	1 cut(s)	366
BstHHI	GCGC	1 cut(s)	365
BstMAI	GTCTC	7 cut(s)	158, 1458, 1487, 1792, 1828, 1889, 2387
BstMWI	GCNNNNNNNGC	9 cut(s)	68, 88, 371, 1015, 1739, 1778, 1787, 2230, 2252
BstNI	CCWGG	2 cut(s)	1109, 1317
BstNSI	RCATGY	2 cut(s)	863, 1018
BstSCI	CCNGG	2 cut(s)	1107, 1315
BstSFI	CTRYAG	5 cut(s)	692, 1127, 1212, 1946, 2300
BstSLI	GKGCMC	2 cut(s)	1382, 2570
BstSNI	TACGTA	1 cut(s)	1400
BstV1I	GCAGC	8 cut(s)	49, 91, 502, 1402, 1756, 1759, 1960, 2286
BstV2I	GAAGAC	2 cut(s)	608, 786
BstX2I	RGATCY	4 cut(s)	889, 919, 1205, 1508
BstYI	RGATCY	4 cut(s)	889, 919, 1205, 1508
BstZ17I	GTATAC	1 cut(s)	2614
BsuRI	GGCC	4 cut(s)	647, 963, 1112, 2070
BtgI	CCRYGG	1 cut(s)	57
BtrI	CACGTC	1 cut(s)	1610
BtsCI	GGATG	6 cut(s)	300, 388, 550, 718, 1464, 1636
BtsIMutI	CAGTG	2 cut(s)	536, 790
Cac8I	GCNNGC	1 cut(s)	1767
CciI	TCATGA	1 cut(s)	967
CfoI	GCGC	1 cut(s)	365
Cfr13I	GGNCC	3 cut(s)	928, 1552, 2591
CseI	GACGC	1 cut(s)	23
Csp6I	GTAC	5 cut(s)	306, 432, 1245, 2027, 2543
CspCI	CAANNNNNGTGG	2 cut(s)	2165, 2200
CviQI	GTAC	5 cut(s)	306, 432, 1245, 2027, 2543
DraI	TTTAAA	4 cut(s)	619, 1345, 1563, 2364
Eam1104I	CTCTTC	2 cut(s)	264, 1293
EarI	CTCTTC	2 cut(s)	264, 1293
Eco105I	TACGTA	1 cut(s)	1400
Eco130I	CCWWGG	1 cut(s)	57
Eco147I	AGGCCT	2 cut(s)	963, 1112
Eco24I	GRGCYC	1 cut(s)	1672
Eco47I	GGWCC	3 cut(s)	928, 1552, 2591
Eco57I	CTGAAG	1 cut(s)	1797
Eco88I	CYCGRG	1 cut(s)	2569
EcoRII	CCWGG	2 cut(s)	1107, 1315
EcoT14I	CCWWGG	1 cut(s)	57
EcoT22I	ATGCAT	1 cut(s)	550
EcoT38I	GRGCYC	1 cut(s)	1672
ErhI	CCWWGG	1 cut(s)	57
Esp3I	CGTCTC	1 cut(s)	2387
FalI	AAGNNNNNCTT	4 cut(s)	717, 749, 2359, 2391
FauNDI	CATATG	2 cut(s)	232, 1912
FblI	GTMKAC	2 cut(s)	908, 2613
FokI	GGATG	6 cut(s)	287, 395, 557, 725, 1451, 1643
FriOI	GRGCYC	1 cut(s)	1672
FspBI	CTAG	5 cut(s)	1002, 1227, 1644, 1766, 2312
GlaI	GCGC	1 cut(s)	364
HaeII	RGCGCY	1 cut(s)	366
HaeIII	GGCC	4 cut(s)	647, 963, 1112, 2070
HgaI	GACGC	1 cut(s)	23
HhaI	GCGC	1 cut(s)	365
Hin1I	GRCGYC	1 cut(s)	34
Hin6I	GCGC	1 cut(s)	363
HinP1I	GCGC	1 cut(s)	363
HincII	GTYRAC	1 cut(s)	794
HindII	GTYRAC	1 cut(s)	794
HindIII	AAGCTT	6 cut(s)	110, 933, 1347, 1812, 1982, 2420
HphI	GGTGA	5 cut(s)	26, 45, 782, 1075, 1492
Hpy166II	GTNNAC	3 cut(s)	794, 909, 2614
Hpy8I	GTNNAC	3 cut(s)	794, 909, 2614
HpyAV	CCTTC	8 cut(s)	208, 535, 974, 1132, 1432, 1471, 2214, 2311
HpyCH4III	ACNGT	8 cut(s)	124, 250, 323, 398, 704, 785, 829, 2325
HpyCH4IV	ACGT	4 cut(s)	739, 1399, 1609, 2183
HpyF10VI	GCNNNNNNNGC	9 cut(s)	68, 88, 371, 1015, 1739, 1778, 1787, 2230, 2252
HpySE526I	ACGT	4 cut(s)	739, 1399, 1609, 2183
Hsp92I	GRCGYC	1 cut(s)	34
HspAI	GCGC	1 cut(s)	363
LmnI	GCTCC	5 cut(s)	6, 178, 370, 478, 1165
Lsp1109I	GCAGC	8 cut(s)	49, 91, 502, 1402, 1756, 1759, 1960, 2286
MaeI	CTAG	5 cut(s)	1002, 1227, 1644, 1766, 2312
MaeII	ACGT	4 cut(s)	739, 1399, 1609, 2183
MaeIII	GTNAC	5 cut(s)	35, 704, 808, 1181, 2235
MbiI	CCGCTC	1 cut(s)	2105
MfeI	CAATTG	3 cut(s)	519, 1899, 2409
MflI	RGATCY	4 cut(s)	889, 919, 1205, 1508
MhlI	GDGCHC	6 cut(s)	11, 183, 475, 1382, 1672, 2570
MlyI	GAGTC	4 cut(s)	26, 160, 914, 1583
MmeI	TCCRAC	2 cut(s)	2251, 2286
Mph1103I	ATGCAT	1 cut(s)	550
MroXI	GAANNNNTTC	3 cut(s)	1207, 2374, 2421
MspCI	CTTAAG	1 cut(s)	1019
MspR9I	CCNGG	2 cut(s)	1109, 1317
MunI	CAATTG	3 cut(s)	519, 1899, 2409
Mva1269I	GAATGC	2 cut(s)	1661, 2026
MvaI	CCWGG	2 cut(s)	1109, 1317
MwoI	GCNNNNNNNGC	9 cut(s)	68, 88, 371, 1015, 1739, 1778, 1787, 2230, 2252
NcoI	CCATGG	1 cut(s)	57
NdeI	CATATG	2 cut(s)	232, 1912
NheI	GCTAGC	1 cut(s)	1765
NlaIV	GGNNCC	1 cut(s)	1381
NmuCI	GTSAC	3 cut(s)	35, 704, 1181
NsiI	ATGCAT	1 cut(s)	550
NspI	RCATGY	2 cut(s)	863, 1018
NspV	TTCGAA	2 cut(s)	1201, 2218
PagI	TCATGA	1 cut(s)	967
PceI	AGGCCT	2 cut(s)	963, 1112
PcsI	WCGNNNNNNNCGW	1 cut(s)	31
PctI	GAATGC	2 cut(s)	1661, 2026
PdmI	GAANNNNTTC	3 cut(s)	1207, 2374, 2421
PfeI	GAWTC	8 cut(s)	236, 290, 314, 1223, 1622, 1649, 2059, 2109
PflMI	CCANNNNNTGG	1 cut(s)	584
PleI	GAGTC	4 cut(s)	25, 160, 913, 1582
PpsI	GAGTC	4 cut(s)	25, 160, 913, 1582
Ppu21I	YACGTR	2 cut(s)	1400, 2184
Psp1406I	AACGTT	1 cut(s)	739
Psp6I	CCWGG	2 cut(s)	1107, 1315
PspGI	CCWGG	2 cut(s)	1107, 1315
PspN4I	GGNNCC	1 cut(s)	1381
PspPI	GGNCC	3 cut(s)	928, 1552, 2591
PstI	CTGCAG	4 cut(s)	696, 1216, 1950, 2304
PsuI	RGATCY	4 cut(s)	889, 919, 1205, 1508
RsaI	GTAC	5 cut(s)	307, 433, 1246, 2028, 2544
RsaNI	GTAC	5 cut(s)	306, 432, 1245, 2027, 2543
Sau96I	GGNCC	3 cut(s)	928, 1552, 2591
ScaI	AGTACT	1 cut(s)	2544
SchI	GAGTC	4 cut(s)	26, 160, 914, 1583
ScrFI	CCNGG	2 cut(s)	1109, 1317
SduI	GDGCHC	6 cut(s)	11, 183, 475, 1382, 1672, 2570
SfcI	CTRYAG	5 cut(s)	692, 1127, 1212, 1946, 2300
SfuI	TTCGAA	2 cut(s)	1201, 2218
SinI	GGWCC	3 cut(s)	928, 1552, 2591
SmlI	CTYRAG	5 cut(s)	191, 637, 914, 1019, 1795
SmoI	CTYRAG	5 cut(s)	191, 637, 914, 1019, 1795
SnaBI	TACGTA	1 cut(s)	1400
SseBI	AGGCCT	2 cut(s)	963, 1112
SsiI	CCGC	3 cut(s)	816, 2103, 2253
SspI	AATATT	2 cut(s)	496, 987
SspMI	CTAG	5 cut(s)	1002, 1227, 1644, 1766, 2312
StuI	AGGCCT	2 cut(s)	963, 1112
StyD4I	CCNGG	2 cut(s)	1107, 1315
StyI	CCWWGG	1 cut(s)	57
TaaI	ACNGT	8 cut(s)	124, 250, 323, 398, 704, 785, 829, 2325
TaiI	ACGT	4 cut(s)	742, 1402, 1612, 2186
TatI	WGTACW	3 cut(s)	431, 2026, 2542
TauI	GCSGC	2 cut(s)	2105, 2255
TfiI	GAWTC	8 cut(s)	236, 290, 314, 1223, 1622, 1649, 2059, 2109
TscAI	CASTG	2 cut(s)	536, 790
TseFI	GTSAC	3 cut(s)	35, 704, 1181
TseI	GCWGC	8 cut(s)	62, 79, 515, 1415, 1769, 1772, 1948, 2299
Tsp45I	GTSAC	3 cut(s)	35, 704, 1181
TspGWI	ACGGA	3 cut(s)	169, 293, 2411
TspRI	CASTG	2 cut(s)	536, 790
Van91I	CCANNNNNTGG	1 cut(s)	584
Vha464I	CTTAAG	1 cut(s)	1019
VpaK11BI	GGWCC	3 cut(s)	928, 1552, 2591
XapI	RAATTY	4 cut(s)	200, 1230, 1340, 1805
XbaI	TCTAGA	1 cut(s)	1226
XceI	RCATGY	2 cut(s)	863, 1018
XcmI	CCANNNNNNNNNTGG	1 cut(s)	247
XmiI	GTMKAC	2 cut(s)	908, 2613
XmnI	GAANNNNTTC	3 cut(s)	1207, 2374, 2421
XspI	CTAG	5 cut(s)	1002, 1227, 1644, 1766, 2312
ZrmI	AGTACT	1 cut(s)	2544
Zsp2I	ATGCAT	1 cut(s)	550

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.