Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0014361

Physical Location & Seq

Genomic Coordinates Forward (+)

25475560 .. 25478311

Gene Structure 2752 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1219200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1596 bp

ATGCTTCCCCAAACTTCCGTCCACCACCACCACAACGCCACTCTCCCCTACTTCCTCCCCTTCCGGTACAAAATCCCAACTCTGACCCTCCCTACTTTCCTAAATTTCCAAAAACTCCCCACCATCTCATGCTCCATATCCCAAGTCCACAACTATGGCACTGTGGACTACGAGCGGAGGCCCATGGTCAAATGGAATGCCATTTACCGGAAAATATCGCTCACGGAGGATCCCCAAGTCCGGTCTGCAGACGTGTTGAACCAGTGGGAGAAGGAGGGCAGGAAGCTCACCAAGTGGGAGCTTTGTAGAGTGGTCAAGGAATTGAGGAAGTACAAGCGCTATGATAGAGCTCTTGAGGTGTATGATTGGATGAGCAACAGAGGAGAGAGGTTTAGAATATCCACTAGTGATGCTGCAATTCAACTAGATTTAGTTGCTAAAGTTCGGGGAGTTGCTAGTGCTGAAAATTACTTCCTGAGCCTGCCAGGTACATTAAAGGACAGGAGAATATATGGGGCTCTACTCAATGCCTATGTTCGGGCTAGAATGAAAGAGAAGGCAGAAGCATTACTGGATAAAATGAGAACTAAGGGGCATGCATTGCAATCTCTTCCATTTAATGTGATGATGACACTTTATATGAATCTCAAAGACTATGATAAAGTTGATTCGATTATTTCTGAAATGCTAGAGAAAAACATTCAGCTAGATATATACTCCTACAATATCTGGTTATCGTCTCGTGGATCCCAAGGATCTGCAGAACGGATGGAAGAGGTTTTTGAAAAGATGAAAGTGGACAGAACCATTATTCCCAACTGGACCACATTCAGCACAATGGCTACAATGTACATCAAGATGGGGCAGCTTGACAAGGCTGAAGCTTGCCTAAGGAAGGTTGAGAGTAGGATCACAGGTCGGGATCGGATACCTTATCATTATCTCTTAAGTCTTTATGGCAGTGTTGGCAAGAAAGAGGAGGCTTATCGGGTGTGGAATGTATACAAAGCAAGTTTTCCCAGTATTCCGAATTTGGGTTACCATGCTATCATGTCTTCTCTACTCAGATTAGGTGACATTGAAGGGGCTGAAAAGCTTTATGAGGAATGGCTGACGGTTAAGTCAACATACGACCCTAGAATTGCAAATCTTTTCATCACATTTTATATTAAAGAAGGGGATTTTGTTAATGCCCAAAGTTTCTACGATCACATGGTTGATGTAGGAGGAAAACCTAATTCAAGTACATGGGAGGCCCTTGCTGAAGGACATATTGCAGAGAAGAGAATTTCTGAGGCATTATCCTGCTGGAAAGAAGCTTTTTCTGCTGAGGGATCAAAGAGTTGGAGACCAAAGCCCGTAAATGTTTCTGCCTTCCTTGAACTCTGTGAGCAAGAAGGTGATTCGGTAAGCAAAGAGGTATTCATGGGACTTTTGAGTCAGTCAGGACAGCTTAGAAATAAGTTATATGTATCACTTCTTGGCTTAGCGGATGAAGATGTTAATTATAAAACTAACGTTAATGAGGATGATGATGAGAAGGCTGATGATGGATCAGAATTTCTTCTGAACCAATTGCAGGGTACTGCCCCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR TPR_8

Protein Analysis

532

Amino Acids

61.17

Weight (kDa)

8.03

Isoelectric Point (pI)

41.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	206 - 245	3.7e-06	PPR repeat family
PPR_3	PF13812	224 - 284	1.4e-07	Pentatricopeptide repeat domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014361)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02150
fragaria_vesca	FvH4_7g09350
malus_domestica	MD02G1219200.v1.1 MD07G1097800.v1.1
prunus_persica	Prupe.2G121900_v2.0.a1
pyrus_communis	pycom02g18110 pycom07g08050
rosa_chinensis	RchiOBHm_Chr1g0346001
rosa_laevigata	RLG00000028826
rosa_multiflora	Rmu_sc0008115.1_g000003
rosa_roxburghii	Rroxscaffold_4G00308950
rosa_samantha	Rh1AG196500 Rh1BG162000 Rh1CG181400 Rh1DG192300
rosa_wichuraiana	Rw1G016400 Rw1G016520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1509
AasI	GACNNNNNNGTC	2 cut(s)	1120, 1437
AccBSI	CCGCTC	1 cut(s)	175
AccI	GTMKAC	1 cut(s)	1002
AciI	CCGC	2 cut(s)	175, 1490
AclI	AACGTT	1 cut(s)	1518
AclWI	GGATC	9 cut(s)	224, 237, 741, 754, 763, 917, 930, 1342, 1561
AcsI	RAATTY	4 cut(s)	103, 1030, 1287, 1559
AcuI	CTGAAG	2 cut(s)	900, 1284
AdeI	CACNNNGTG	1 cut(s)	294
AfaI	GTAC	6 cut(s)	68, 332, 490, 851, 1246, 1585
AfeI	AGCGCT	1 cut(s)	338
AfiI	CCNNNNNNNGG	6 cut(s)	207, 240, 537, 895, 1034, 1579
AflII	CTTAAG	1 cut(s)	946
AflIII	ACRYGT	1 cut(s)	252
AgsI	TTSAA	6 cut(s)	259, 422, 785, 1082, 1242, 1382
AhlI	ACTAGT	1 cut(s)	404
AjiI	CACGTC	1 cut(s)	253
AjnI	CCWGG	1 cut(s)	484
AloI	GAACNNNNNNTCC	2 cut(s)	796, 828
AluBI	AGCT	9 cut(s)	286, 301, 350, 706, 868, 884, 1096, 1319, 1453
AluI	AGCT	9 cut(s)	286, 301, 350, 706, 868, 884, 1096, 1319, 1453
Alw21I	GWGCWC	1 cut(s)	352
Alw26I	GTCTC	2 cut(s)	744, 1342
AlwI	GGATC	9 cut(s)	224, 237, 741, 754, 763, 917, 930, 1342, 1561
Aor51HI	AGCGCT	1 cut(s)	338
AoxI	GGCC	2 cut(s)	179, 1254
ApeKI	GCWGC	2 cut(s)	413, 865
ApoI	RAATTY	4 cut(s)	103, 1030, 1287, 1559
Asp700I	GAANNNNTTC	1 cut(s)	1563
AspLEI	GCGC	1 cut(s)	339
AspS9I	GGNCC	3 cut(s)	180, 822, 1255
AsuHPI	GGTGA	3 cut(s)	280, 1085, 1412
AvaII	GGWCC	1 cut(s)	822
AxyI	CCTNAGG	1 cut(s)	890
BamHI	GGATCC	2 cut(s)	229, 746
BanII	GRGCYC	2 cut(s)	352, 520
BauI	CACGAG	1 cut(s)	741
BbsI	GAAGAC	1 cut(s)	1047
Bbv12I	GWGCWC	1 cut(s)	352
BbvCI	CCTCAGC	1 cut(s)	1329
BbvI	GCAGC	2 cut(s)	400, 877
BccI	CCATC	4 cut(s)	131, 763, 853, 1544
BciT130I	CCWGG	1 cut(s)	486
BciVI	GTATCC	1 cut(s)	921
BcoDI	GTCTC	2 cut(s)	744, 1342
BcuI	ACTAGT	1 cut(s)	404
BfaI	CTAG	7 cut(s)	405, 425, 456, 543, 689, 707, 1137
BfmI	CTRYAG	2 cut(s)	246, 759
BfoI	RGCGCY	1 cut(s)	340
BfrI	CTTAAG	1 cut(s)	946
BfuI	GTATCC	1 cut(s)	921
BisI	GCNGC	2 cut(s)	414, 866
BlpI	GCTNAGC	1 cut(s)	1486
BlsI	GCNGC	2 cut(s)	415, 867
Bme1390I	CCNGG	1 cut(s)	486
Bme18I	GGWCC	1 cut(s)	822
BmgBI	CACGTC	1 cut(s)	253
BmgT120I	GGNCC	3 cut(s)	180, 822, 1255
BmiI	GGNNCC	2 cut(s)	231, 748
BmrFI	CCNGG	1 cut(s)	486
BmrI	ACTGGG	1 cut(s)	1014
BmsI	GCATC	1 cut(s)	400
BmuI	ACTGGG	1 cut(s)	1014
BpiI	GAAGAC	1 cut(s)	1047
Bpu10I	CCTNAGC	2 cut(s)	476, 1329
Bpu1102I	GCTNAGC	1 cut(s)	1486
BpuEI	CTTGAG	1 cut(s)	374
BsaI	GGTCTC	1 cut(s)	1342
BsaJI	CCNNGG	2 cut(s)	183, 751
BsaWI	WCCGGW	3 cut(s)	63, 207, 240
Bsc4I	CCNNNNNNNGG	6 cut(s)	207, 240, 537, 895, 1034, 1579
Bse1I	ACTGG	4 cut(s)	262, 576, 824, 1020
Bse21I	CCTNAGG	1 cut(s)	890
Bse3DI	GCAATG	1 cut(s)	599
BseBI	CCWGG	1 cut(s)	486
BseDI	CCNNGG	2 cut(s)	183, 751
BseGI	GGATG	4 cut(s)	375, 774, 1498, 1534
BseLI	CCNNNNNNNGG	6 cut(s)	207, 240, 537, 895, 1034, 1579
BseMI	GCAATG	1 cut(s)	599
BseMII	CTCAG	4 cut(s)	467, 1078, 1284, 1320
BseNI	ACTGG	4 cut(s)	262, 576, 824, 1020
BseRI	GAGGAG	2 cut(s)	396, 992
BseXI	GCAGC	2 cut(s)	400, 877
BshFI	GGCC	2 cut(s)	181, 1256
BsiHKAI	GWGCWC	1 cut(s)	352
BsiSI	CCGG	3 cut(s)	64, 208, 241
BslFI	GGGAC	1 cut(s)	1443
BslI	CCNNNNNNNGG	6 cut(s)	207, 240, 537, 895, 1034, 1579
BsmAI	GTCTC	2 cut(s)	744, 1342
BsmBI	CGTCTC	1 cut(s)	744
BsmFI	GGGAC	1 cut(s)	1443
BsmI	GAATGC	1 cut(s)	202
BsnI	GGCC	2 cut(s)	181, 1256
Bso31I	GGTCTC	1 cut(s)	1342
Bsp1286I	GDGCHC	2 cut(s)	352, 520
Bsp1407I	TGTACA	1 cut(s)	849
Bsp143I	GATC	8 cut(s)	229, 746, 755, 909, 922, 1207, 1334, 1553
Bsp1720I	GCTNAGC	1 cut(s)	1486
Bsp19I	CCATGG	1 cut(s)	183
BspACI	CCGC	2 cut(s)	175, 1490
BspANI	GGCC	2 cut(s)	181, 1256
BspCNI	CTCAG	4 cut(s)	468, 1077, 1285, 1321
BspLI	GGNNCC	2 cut(s)	231, 748
BspMAI	CTGCAG	2 cut(s)	250, 763
BspPI	GGATC	9 cut(s)	224, 237, 741, 754, 763, 917, 930, 1342, 1561
BspTI	CTTAAG	1 cut(s)	946
BspTNI	GGTCTC	1 cut(s)	1342
BsrBI	CCGCTC	1 cut(s)	175
BsrDI	GCAATG	1 cut(s)	599
BsrGI	TGTACA	1 cut(s)	849
BsrI	ACTGG	4 cut(s)	262, 576, 824, 1020
BssECI	CCNNGG	2 cut(s)	183, 751
BssMI	GATC	8 cut(s)	229, 746, 755, 909, 922, 1207, 1334, 1553
BssNAI	GTATAC	1 cut(s)	1003
BssSI	CACGAG	1 cut(s)	741
BssT1I	CCWWGG	2 cut(s)	183, 751
Bst1107I	GTATAC	1 cut(s)	1003
Bst2BI	CACGAG	1 cut(s)	741
Bst2UI	CCWGG	1 cut(s)	486
Bst4CI	ACNGT	2 cut(s)	163, 1117
Bst6I	CTCTTC	3 cut(s)	615, 768, 1277
BstAFI	CTTAAG	1 cut(s)	946
BstAPI	GCANNNNNTGC	1 cut(s)	601
BstAUI	TGTACA	1 cut(s)	849
BstC8I	GCNNGC	3 cut(s)	482, 597, 886
BstDEI	CTNAG	8 cut(s)	476, 588, 890, 1064, 1293, 1329, 1454, 1486
BstDSI	CCRYGG	1 cut(s)	183
BstEII	GGTNACC	1 cut(s)	1037
BstENI	CCTNNNNNAGG	1 cut(s)	893
BstF5I	GGATG	4 cut(s)	375, 774, 1498, 1534
BstH2I	RGCGCY	1 cut(s)	340
BstHHI	GCGC	1 cut(s)	339
BstKTI	GATC	8 cut(s)	232, 749, 758, 912, 925, 1210, 1337, 1556
BstMAI	GTCTC	2 cut(s)	744, 1342
BstMBI	GATC	8 cut(s)	229, 746, 755, 909, 922, 1207, 1334, 1553
BstMWI	GCNNNNNNNGC	3 cut(s)	601, 966, 1325
BstNI	CCWGG	1 cut(s)	486
BstNSI	RCATGY	1 cut(s)	599
BstPI	GGTNACC	1 cut(s)	1037
BstSCI	CCNGG	1 cut(s)	484
BstSFI	CTRYAG	2 cut(s)	246, 759
BstV1I	GCAGC	2 cut(s)	400, 877
BstV2I	GAAGAC	1 cut(s)	1047
BstX2I	RGATCY	3 cut(s)	229, 746, 755
BstXI	CCANNNNNNTGG	1 cut(s)	155
BstYI	RGATCY	3 cut(s)	229, 746, 755
BstZ17I	GTATAC	1 cut(s)	1003
Bsu36I	CCTNAGG	1 cut(s)	890
BsuI	GTATCC	1 cut(s)	921
BsuRI	GGCC	2 cut(s)	181, 1256
BtgI	CCRYGG	1 cut(s)	183
BtrI	CACGTC	1 cut(s)	253
BtsCI	GGATG	4 cut(s)	375, 774, 1498, 1534
BtsI	GCAGTG	1 cut(s)	967
BtsIMutI	CAGTG	3 cut(s)	159, 269, 967
Cac8I	GCNNGC	3 cut(s)	482, 597, 886
CfoI	GCGC	1 cut(s)	339
Cfr13I	GGNCC	3 cut(s)	180, 822, 1255
Csp6I	GTAC	6 cut(s)	67, 331, 489, 850, 1245, 1584
CviAII	CATG	8 cut(s)	129, 184, 596, 1043, 1051, 1213, 1248, 1426
CviQI	GTAC	6 cut(s)	67, 331, 489, 850, 1245, 1584
DdeI	CTNAG	8 cut(s)	476, 588, 890, 1064, 1293, 1329, 1454, 1486
DpnI	GATC	8 cut(s)	231, 748, 757, 911, 924, 1209, 1336, 1555
DpnII	GATC	8 cut(s)	229, 746, 755, 909, 922, 1207, 1334, 1553
DraIII	CACNNNGTG	1 cut(s)	294
DrdI	GACNNNNNNGTC	2 cut(s)	1120, 1437
DseDI	GACNNNNNNGTC	2 cut(s)	1120, 1437
Eam1104I	CTCTTC	3 cut(s)	615, 768, 1277
EarI	CTCTTC	3 cut(s)	615, 768, 1277
Ecl136II	GAGCTC	1 cut(s)	350
Eco130I	CCWWGG	2 cut(s)	183, 751
Eco24I	GRGCYC	2 cut(s)	352, 520
Eco31I	GGTCTC	1 cut(s)	1342
Eco47I	GGWCC	1 cut(s)	822
Eco47III	AGCGCT	1 cut(s)	338
Eco53kI	GAGCTC	1 cut(s)	350
Eco57I	CTGAAG	2 cut(s)	900, 1284
Eco81I	CCTNAGG	1 cut(s)	890
Eco91I	GGTNACC	1 cut(s)	1037
EcoICRI	GAGCTC	1 cut(s)	350
EcoNI	CCTNNNNNAGG	1 cut(s)	893
EcoO109I	RGGNCCY	1 cut(s)	1255
EcoO65I	GGTNACC	1 cut(s)	1037
EcoRII	CCWGG	1 cut(s)	484
EcoT14I	CCWWGG	2 cut(s)	183, 751
EcoT22I	ATGCAT	1 cut(s)	601
EcoT38I	GRGCYC	2 cut(s)	352, 520
ErhI	CCWWGG	2 cut(s)	183, 751
Esp3I	CGTCTC	1 cut(s)	744
FaeI	CATG	8 cut(s)	132, 187, 599, 1046, 1054, 1216, 1251, 1429
FaqI	GGGAC	1 cut(s)	1443
FatI	CATG	8 cut(s)	128, 183, 595, 1042, 1050, 1212, 1247, 1425
FblI	GTMKAC	1 cut(s)	1002
Fnu4HI	GCNGC	2 cut(s)	414, 866
FokI	GGATG	4 cut(s)	382, 781, 1505, 1541
FriOI	GRGCYC	2 cut(s)	352, 520
Fsp4HI	GCNGC	2 cut(s)	414, 866
FspBI	CTAG	7 cut(s)	405, 425, 456, 543, 689, 707, 1137
GlaI	GCGC	1 cut(s)	338
GluI	GCNGC	2 cut(s)	414, 866
HaeII	RGCGCY	1 cut(s)	340
HaeIII	GGCC	2 cut(s)	181, 1256
HapII	CCGG	3 cut(s)	64, 208, 241
HhaI	GCGC	1 cut(s)	339
Hin1II	CATG	8 cut(s)	132, 187, 599, 1046, 1054, 1216, 1251, 1429
Hin6I	GCGC	1 cut(s)	337
HinP1I	GCGC	1 cut(s)	337
HincII	GTYRAC	1 cut(s)	1125
HindII	GTYRAC	1 cut(s)	1125
HindIII	AAGCTT	3 cut(s)	882, 1094, 1317
HinfI	GANTC	4 cut(s)	643, 668, 1403, 1438
HpaII	CCGG	3 cut(s)	64, 208, 241
HphI	GGTGA	3 cut(s)	280, 1085, 1412
Hpy166II	GTNNAC	6 cut(s)	22, 148, 166, 799, 1003, 1125
Hpy188I	TCNGA	8 cut(s)	84, 682, 927, 1029, 1067, 1294, 1558, 1569
Hpy188III	TCNNGA	5 cut(s)	353, 475, 856, 920, 1446
Hpy8I	GTNNAC	6 cut(s)	22, 148, 166, 799, 1003, 1125
HpyCH4III	ACNGT	2 cut(s)	163, 1117
HpyCH4IV	ACGT	2 cut(s)	252, 1518
HpyCH4V	TGCA	8 cut(s)	248, 416, 599, 604, 761, 1145, 1277, 1579
HpyF10VI	GCNNNNNNNGC	3 cut(s)	601, 966, 1325
HpyF3I	CTNAG	8 cut(s)	476, 588, 890, 1064, 1293, 1329, 1454, 1486
HpySE526I	ACGT	2 cut(s)	252, 1518
Hsp92II	CATG	8 cut(s)	132, 187, 599, 1046, 1054, 1216, 1251, 1429
HspAI	GCGC	1 cut(s)	337
Kzo9I	GATC	8 cut(s)	229, 746, 755, 909, 922, 1207, 1334, 1553
LmnI	GCTCC	2 cut(s)	137, 298
Lsp1109I	GCAGC	2 cut(s)	400, 877
LweI	GCATC	1 cut(s)	400
MaeI	CTAG	7 cut(s)	405, 425, 456, 543, 689, 707, 1137
MaeII	ACGT	2 cut(s)	252, 1518
MaeIII	GTNAC	2 cut(s)	1037, 1073
MalI	GATC	8 cut(s)	231, 748, 757, 911, 924, 1209, 1336, 1555
MbiI	CCGCTC	1 cut(s)	175
MboI	GATC	8 cut(s)	229, 746, 755, 909, 922, 1207, 1334, 1553
MboII	GAAGA	6 cut(s)	602, 785, 1047, 1294, 1508, 1556
MfeI	CAATTG	1 cut(s)	1574
MflI	RGATCY	3 cut(s)	229, 746, 755
MhlI	GDGCHC	2 cut(s)	352, 520
MlyI	GAGTC	1 cut(s)	1447
MmeI	TCCRAC	1 cut(s)	1325
Mph1103I	ATGCAT	1 cut(s)	601
MroXI	GAANNNNTTC	1 cut(s)	1563
MseI	TTAA	8 cut(s)	494, 618, 947, 1119, 1170, 1188, 1503, 1521
MslI	CAYNNNNRTG	2 cut(s)	153, 857
MspCI	CTTAAG	1 cut(s)	946
MspI	CCGG	3 cut(s)	64, 208, 241
MspR9I	CCNGG	1 cut(s)	486
MunI	CAATTG	1 cut(s)	1574
Mva1269I	GAATGC	1 cut(s)	202
MvaI	CCWGG	1 cut(s)	486
MwoI	GCNNNNNNNGC	3 cut(s)	601, 966, 1325
NcoI	CCATGG	1 cut(s)	183
NdeII	GATC	8 cut(s)	229, 746, 755, 909, 922, 1207, 1334, 1553
NlaIII	CATG	8 cut(s)	132, 187, 599, 1046, 1054, 1216, 1251, 1429
NlaIV	GGNNCC	2 cut(s)	231, 748
NmuCI	GTSAC	1 cut(s)	1073
NsiI	ATGCAT	1 cut(s)	601
NspI	RCATGY	1 cut(s)	599
PaeI	GCATGC	1 cut(s)	599
PctI	GAATGC	1 cut(s)	202
PdmI	GAANNNNTTC	1 cut(s)	1563
PfeI	GAWTC	3 cut(s)	643, 668, 1403
PkrI	GCNGC	2 cut(s)	415, 867
PleI	GAGTC	1 cut(s)	1446
PpsI	GAGTC	1 cut(s)	1446
PsiI	TTATAA	1 cut(s)	1509
Psp124BI	GAGCTC	1 cut(s)	352
Psp1406I	AACGTT	1 cut(s)	1518
Psp6I	CCWGG	1 cut(s)	484
PspEI	GGTNACC	1 cut(s)	1037
PspGI	CCWGG	1 cut(s)	484
PspN4I	GGNNCC	2 cut(s)	231, 748
PspPI	GGNCC	3 cut(s)	180, 822, 1255
PstI	CTGCAG	2 cut(s)	250, 763
PsuI	RGATCY	3 cut(s)	229, 746, 755
RsaI	GTAC	6 cut(s)	68, 332, 490, 851, 1246, 1585
RsaNI	GTAC	6 cut(s)	67, 331, 489, 850, 1245, 1584
RseI	CAYNNNNRTG	2 cut(s)	153, 857
SacI	GAGCTC	1 cut(s)	352
SaqAI	TTAA	8 cut(s)	494, 618, 947, 1119, 1170, 1188, 1503, 1521
SatI	GCNGC	2 cut(s)	414, 866
Sau3AI	GATC	8 cut(s)	229, 746, 755, 909, 922, 1207, 1334, 1553
Sau96I	GGNCC	3 cut(s)	180, 822, 1255
SchI	GAGTC	1 cut(s)	1447
ScrFI	CCNGG	1 cut(s)	486
SduI	GDGCHC	2 cut(s)	352, 520
SfaNI	GCATC	1 cut(s)	400
SfcI	CTRYAG	2 cut(s)	246, 759
SinI	GGWCC	1 cut(s)	822
SmiMI	CAYNNNNRTG	2 cut(s)	153, 857
SmlI	CTYRAG	2 cut(s)	353, 946
SmoI	CTYRAG	2 cut(s)	353, 946
SpeI	ACTAGT	1 cut(s)	404
SphI	GCATGC	1 cut(s)	599
SsiI	CCGC	2 cut(s)	175, 1490
SspMI	CTAG	7 cut(s)	405, 425, 456, 543, 689, 707, 1137
SstI	GAGCTC	1 cut(s)	352
StyD4I	CCNGG	1 cut(s)	484
StyI	CCWWGG	2 cut(s)	183, 751
TaaI	ACNGT	2 cut(s)	163, 1117
TaiI	ACGT	2 cut(s)	255, 1521
TaqI	TCGA	1 cut(s)	671
TatI	WGTACW	3 cut(s)	330, 849, 1244
TfiI	GAWTC	3 cut(s)	643, 668, 1403
Tru1I	TTAA	8 cut(s)	494, 618, 947, 1119, 1170, 1188, 1503, 1521
Tru9I	TTAA	8 cut(s)	494, 618, 947, 1119, 1170, 1188, 1503, 1521
TscAI	CASTG	3 cut(s)	166, 269, 967
TseFI	GTSAC	1 cut(s)	1073
TseI	GCWGC	2 cut(s)	413, 865
Tsp45I	GTSAC	1 cut(s)	1073
TspDTI	ATGAA	6 cut(s)	563, 656, 806, 1144, 1414, 1509
TspGWI	ACGGA	3 cut(s)	7, 239, 781
TspRI	CASTG	3 cut(s)	166, 269, 967
Vha464I	CTTAAG	1 cut(s)	946
VpaK11BI	GGWCC	1 cut(s)	822
XagI	CCTNNNNNAGG	1 cut(s)	893
XapI	RAATTY	4 cut(s)	103, 1030, 1287, 1559
XceI	RCATGY	1 cut(s)	599
XmiI	GTMKAC	1 cut(s)	1002
XmnI	GAANNNNTTC	1 cut(s)	1563
XspI	CTAG	7 cut(s)	405, 425, 456, 543, 689, 707, 1137
Zsp2I	ATGCAT	1 cut(s)	601

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.