MD02G1226600.v1.1

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012336
Physical Location & Seq
Forward (+)
26674309 .. 26678127
3819 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1226600.v1.1.491

Sequence Viewer

Length: 1254 bp
ATGGCTGCCGCCGGAGTCGAGATCAAGCACCCGGACGCCGCGCCGGAGGAGACGTGCTCCGCCAAGGCCTCCAAGCAAGGCGAGGGACTGAGGCAGTACTACCTCCAACACATCCACGAGCTTCAGCTCCAGGTCCGCCAGAAGACTCACAACCTCAATCGCCTCGAAGCTCAGCGCAACGAGCTCAATTCCCGAGTGAGAATGCTCAGGGAAGAACTGCAGCTGCTTCAGGAGCCCGGATCGTATGTTGGTGAAGTTGTTAAAGTAATGGGAAAGAACAAGGTTTTGGTTAAGGTTCATCCAGAAGGGAAATATGTCGTTGATATTGATAAAAACATTGATATCACAAAGATCACACCATCAACAAGAGTTGCTCTCCGTAATGACAGCTACGTGCTACACTTGGTCTTGCCAAGCAAAGTTGATCCTTTGGTCAACCTTATGAAAGTTGAAAAAGTTCCCGATTCTACTTATGATATGATTGGTGGTCTGGATCAGCAAATCAAAGAAATAAAGGAGGTCATTGAGCTTCCAATTAAGCATCCTGAACTGTTCGAGAGTCTTGGTATAGCTCAGCCAAAGGGTGTGCTGCTTTATGGACCACCTGGCACTGGGAAAACACTTTTGGCTAGGGCTGTGGCCCATCACACCGACTGTACATTCATCAGGGTTTCTGGTTCGGAGTTGGTCCAGAAGTACATTGGGGAAGGATCTAGAATGGTCAGAGAACTTTTTGTCATGGCCAGGGAACATGCGCCATCTATCATTTTTATGGATGAAATCGACAGTATCGGATCTGCTCGTATGGAATCTGGTAGTGGCAATGGGGACAGTGAAGTGCAGCGGACCATGCTTGAGCTTCTCAACCAGCTTGATGGGTTTGAGGCATCAAACAAGATCAAGGTCTTAATGGCTACAAATCGTATTGATATTCTGGATCAAGCTCTCCTTAGGCCTGGACGAATTGACAGGAAGATTGAGTTTCCAAATCCTAGTGAAGAGTCTCGTTGGGACATCTTGAAAATTCATTCAAGAAGAATGAATTTGATGCGTGGAATTGACCTGAAGAAGATTGCAGAGAAGATGAATGGTGCATCTGGTGCAGAACTGAAGGCCGTTTGCACAGAGGCTGGAATGTTTGCATTGAGGGAGAGAAGGGTTCACGTAACGCAAGAAGATTTTGAGATGGCAGTGGCCAAGGTAATGAAGAAGGAGAACGAGAAGAACATGTCGTTGCGCAAGTTGTGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0030162 GO:0030163 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

418

Amino Acids

47.06

Weight (kDa)

8.7

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 83 - 138 5.6e-13 Proteasomal ATPase OB C-terminal domain
AAA PF00004 196 - 329 5.4e-44 ATPase family associated with various cellular activities (AAA)
AAA_2 PF07724 196 - 289 1e-05 AAA domain (Cdc48 subfamily)
AAA_lid_PRS2_C PF23902 334 - 403 9.8e-06 PRS2 AAA+ lid C-terminal domain
AAA_lid_3 PF17862 353 - 395 1.5e-14 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012336)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19990 AT5G19990 AT5G19990 AT5G20000
fragaria_vesca FvH4_1g03980
malus_domestica MD02G1226600.v1.1 MD07G1087400.v1.1
prunus_persica Prupe.7G237100_v2.0.a1
pyrus_communis pycom02g19560
rosa_chinensis RchiOBHm_Chr2g0089461
rosa_laevigata RLG00000016031
rosa_multiflora Rmu_sc0000197.1_g000006
rosa_roxburghii Rroxscaffold_2G00151910 Rroxscaffold_6G00403280
rosa_rugosa Rorug02G0001000
rosa_samantha Rh2AG046200 Rh2CG046600 Rh2DG046200
rosa_wichuraiana Rw2G003640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1238
AccII CGCG 1 cut(s) 41
AciI CCGC 5 cut(s) 9, 39, 60, 136, 844
AclWI GGATC 6 cut(s) 247, 419, 501, 718, 802, 945
AcoI YGGCCR 2 cut(s) 741, 1194
AcsI RAATTY 2 cut(s) 1023, 1042
AcuI CTGAAG 4 cut(s) 107, 212, 1085, 1130
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 3 cut(s) 98, 658, 698
AfiI CCNNNNNNNGG 1 cut(s) 611
AflIII ACRYGT 1 cut(s) 1227
AgsI TTSAA 3 cut(s) 452, 1021, 1032
AjiI CACGTC 1 cut(s) 54
AjnI CCWGG 4 cut(s) 129, 604, 743, 955
AjuI GAANNNNNNNTTGG 4 cut(s) 269, 301, 608, 640
Alw21I GWGCWC 2 cut(s) 59, 186
Alw26I GTCTC 2 cut(s) 44, 1008
AlwI GGATC 6 cut(s) 247, 419, 501, 718, 802, 945
AlwNI CAGNNNCTG 1 cut(s) 1130
Ama87I CYCGRG 1 cut(s) 192
AoxI GGCC 6 cut(s) 66, 639, 741, 953, 1113, 1194
ApeKI GCWGC 5 cut(s) 5, 220, 223, 589, 841
ApoI RAATTY 2 cut(s) 1023, 1042
Asp700I GAANNNNTTC 1 cut(s) 456
AspLEI GCGC 4 cut(s) 43, 177, 757, 1239
AspS9I GGNCC 5 cut(s) 133, 599, 640, 688, 846
AsuC2I CCSGG 2 cut(s) 32, 237
AsuHPI GGTGA 1 cut(s) 263
AvaI CYCGRG 1 cut(s) 192
AvaII GGWCC 4 cut(s) 133, 599, 688, 846
AxyI CCTNAGG 1 cut(s) 950
BalI TGGCCA 2 cut(s) 743, 1196
BanII GRGCYC 2 cut(s) 186, 237
BauI CACGAG 1 cut(s) 116
BbsI GAAGAC 1 cut(s) 149
Bbv12I GWGCWC 2 cut(s) 59, 186
BbvI GCAGC 4 cut(s) 210, 232, 576, 853
BccI CCATC 5 cut(s) 367, 651, 766, 869, 1180
BceAI ACGGC 1 cut(s) 1100
BciT130I CCWGG 4 cut(s) 131, 606, 745, 957
BcnI CCSGG 2 cut(s) 32, 237
BcoDI GTCTC 2 cut(s) 44, 1008
BfaI CTAG 3 cut(s) 630, 714, 993
BfmI CTRYAG 1 cut(s) 218
BisI GCNGC 7 cut(s) 6, 9, 39, 221, 224, 590, 842
BlpI GCTNAGC 2 cut(s) 171, 573
BlsI GCNGC 7 cut(s) 7, 10, 40, 222, 225, 591, 843
BmcAI AGTACT 1 cut(s) 98
Bme1390I CCNGG 6 cut(s) 32, 131, 237, 606, 745, 957
Bme18I GGWCC 4 cut(s) 133, 599, 688, 846
BmeT110I CYCGRG 1 cut(s) 192
BmgBI CACGTC 1 cut(s) 54
BmgT120I GGNCC 5 cut(s) 133, 599, 640, 688, 846
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 6 cut(s) 32, 131, 237, 606, 745, 957
BmrI ACTGGG 1 cut(s) 621
BmsI GCATC 4 cut(s) 550, 896, 1038, 1103
BmuI ACTGGG 1 cut(s) 621
BpiI GAAGAC 1 cut(s) 149
BplI GAGNNNNNCTC 4 cut(s) 41, 73, 360, 392
BpmI CTGGAG 1 cut(s) 113
Bpu10I CCTNAGC 1 cut(s) 206
Bpu1102I GCTNAGC 2 cut(s) 171, 573
BpuEI CTTGAG 1 cut(s) 875
BpuMI CCSGG 2 cut(s) 32, 237
BsaAI YACGTR 2 cut(s) 394, 1165
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 3 cut(s) 63, 744, 1197
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 1 cut(s) 611
Bse1I ACTGG 1 cut(s) 616
Bse21I CCTNAGG 1 cut(s) 950
Bse3DI GCAATG 1 cut(s) 829
BseBI CCWGG 4 cut(s) 131, 606, 745, 957
BseDI CCNNGG 3 cut(s) 63, 744, 1197
BseGI GGATG 4 cut(s) 111, 298, 541, 781
BseLI CCNNNNNNNGG 1 cut(s) 611
BseMI GCAATG 1 cut(s) 829
BseMII CTCAG 4 cut(s) 80, 185, 220, 587
BseNI ACTGG 1 cut(s) 616
BseRI GAGGAG 1 cut(s) 62
BseXI GCAGC 4 cut(s) 210, 232, 576, 853
BsgI GTGCAG 2 cut(s) 860, 1122
Bsh1236I CGCG 1 cut(s) 41
BshFI GGCC 6 cut(s) 68, 641, 743, 955, 1115, 1196
BsiHKAI GWGCWC 2 cut(s) 59, 186
BsiHKCI CYCGRG 1 cut(s) 192
BsiSI CCGG 4 cut(s) 12, 32, 44, 237
BslFI GGGAC 3 cut(s) 99, 842, 1025
BslI CCNNNNNNNGG 1 cut(s) 611
BsmAI GTCTC 2 cut(s) 44, 1008
BsmBI CGTCTC 1 cut(s) 44
BsmFI GGGAC 3 cut(s) 99, 842, 1025
BsmI GAATGC 1 cut(s) 207
BsnI GGCC 6 cut(s) 68, 641, 743, 955, 1115, 1196
BsoBI CYCGRG 1 cut(s) 192
Bsp1286I GDGCHC 3 cut(s) 59, 186, 237
Bsp1407I TGTACA 1 cut(s) 656
Bsp143I GATC 9 cut(s) 21, 239, 351, 424, 493, 710, 794, 897, 937
Bsp1720I GCTNAGC 2 cut(s) 171, 573
BspACI CCGC 5 cut(s) 9, 39, 60, 136, 844
BspANI GGCC 6 cut(s) 68, 641, 743, 955, 1115, 1196
BspCNI CTCAG 4 cut(s) 81, 184, 219, 586
BspFNI CGCG 1 cut(s) 41
BspLI GGNNCC 1 cut(s) 234
BspMAI CTGCAG 1 cut(s) 222
BspPI GGATC 6 cut(s) 247, 419, 501, 718, 802, 945
BsrDI GCAATG 1 cut(s) 829
BsrGI TGTACA 1 cut(s) 656
BsrI ACTGG 1 cut(s) 616
BssECI CCNNGG 3 cut(s) 63, 744, 1197
BssMI GATC 9 cut(s) 21, 239, 351, 424, 493, 710, 794, 897, 937
BssNI GRCGYC 1 cut(s) 36
BssSI CACGAG 1 cut(s) 116
BssT1I CCWWGG 2 cut(s) 63, 1197
Bst2BI CACGAG 1 cut(s) 116
Bst2UI CCWGG 4 cut(s) 131, 606, 745, 957
Bst4CI ACNGT 4 cut(s) 552, 656, 788, 833
Bst6I CTCTTC 1 cut(s) 993
BstACI GRCGYC 1 cut(s) 36
BstAPI GCANNNNNTGC 1 cut(s) 1100
BstAUI TGTACA 1 cut(s) 656
BstBAI YACGTR 2 cut(s) 394, 1165
BstDEI CTNAG 5 cut(s) 89, 171, 206, 573, 950
BstF5I GGATG 4 cut(s) 111, 298, 541, 781
BstFNI CGCG 1 cut(s) 41
BstHHI GCGC 4 cut(s) 43, 177, 757, 1239
BstKTI GATC 9 cut(s) 24, 242, 354, 427, 496, 713, 797, 900, 940
BstMAI GTCTC 2 cut(s) 44, 1008
BstMBI GATC 9 cut(s) 21, 239, 351, 424, 493, 710, 794, 897, 937
BstMWI GCNNNNNNNGC 4 cut(s) 181, 232, 850, 1100
BstNI CCWGG 4 cut(s) 131, 606, 745, 957
BstNSI RCATGY 2 cut(s) 755, 1231
BstSCI CCNGG 6 cut(s) 30, 129, 235, 604, 743, 955
BstSFI CTRYAG 1 cut(s) 218
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 4 cut(s) 210, 232, 576, 853
BstV2I GAAGAC 1 cut(s) 149
BstX2I RGATCY 2 cut(s) 710, 794
BstXI CCANNNNNNTGG 1 cut(s) 875
BstYI RGATCY 2 cut(s) 710, 794
Bsu36I CCTNAGG 1 cut(s) 950
BsuRI GGCC 6 cut(s) 68, 641, 743, 955, 1115, 1196
BtrI CACGTC 1 cut(s) 54
BtsCI GGATG 4 cut(s) 111, 298, 541, 781
BtsI GCAGTG 1 cut(s) 1197
BtsIMutI CAGTG 3 cut(s) 609, 838, 1197
CaiI CAGNNNCTG 1 cut(s) 1130
CfoI GCGC 4 cut(s) 43, 177, 757, 1239
Cfr13I GGNCC 5 cut(s) 133, 599, 640, 688, 846
CseI GACGC 1 cut(s) 44
Csp6I GTAC 3 cut(s) 97, 657, 697
CviAII CATG 4 cut(s) 739, 752, 850, 1228
CviQI GTAC 3 cut(s) 97, 657, 697
DdeI CTNAG 5 cut(s) 89, 171, 206, 573, 950
DpnI GATC 9 cut(s) 23, 241, 353, 426, 495, 712, 796, 899, 939
DpnII GATC 9 cut(s) 21, 239, 351, 424, 493, 710, 794, 897, 937
EaeI YGGCCR 2 cut(s) 741, 1194
Eam1104I CTCTTC 1 cut(s) 993
EarI CTCTTC 1 cut(s) 993
EciI GGCGGA 2 cut(s) 49, 125
Ecl136II GAGCTC 1 cut(s) 184
Eco130I CCWWGG 2 cut(s) 63, 1197
Eco147I AGGCCT 2 cut(s) 68, 955
Eco24I GRGCYC 2 cut(s) 186, 237
Eco32I GATATC 1 cut(s) 343
Eco47I GGWCC 4 cut(s) 133, 599, 688, 846
Eco53kI GAGCTC 1 cut(s) 184
Eco57I CTGAAG 4 cut(s) 107, 212, 1085, 1130
Eco81I CCTNAGG 1 cut(s) 950
Eco88I CYCGRG 1 cut(s) 192
EcoICRI GAGCTC 1 cut(s) 184
EcoRII CCWGG 4 cut(s) 129, 604, 743, 955
EcoRV GATATC 1 cut(s) 343
EcoT14I CCWWGG 2 cut(s) 63, 1197
EcoT38I GRGCYC 2 cut(s) 186, 237
ErhI CCWWGG 2 cut(s) 63, 1197
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 4 cut(s) 742, 755, 853, 1231
FalI AAGNNNNNCTT 2 cut(s) 933, 965
FaqI GGGAC 3 cut(s) 99, 842, 1025
FatI CATG 4 cut(s) 738, 751, 849, 1227
Fnu4HI GCNGC 7 cut(s) 6, 9, 39, 221, 224, 590, 842
FokI GGATG 4 cut(s) 98, 285, 528, 788
FriOI GRGCYC 2 cut(s) 186, 237
Fsp4HI GCNGC 7 cut(s) 6, 9, 39, 221, 224, 590, 842
FspBI CTAG 3 cut(s) 630, 714, 993
FspI TGCGCA 1 cut(s) 1238
GlaI GCGC 4 cut(s) 42, 176, 756, 1238
GluI GCNGC 7 cut(s) 6, 9, 39, 221, 224, 590, 842
GsuI CTGGAG 1 cut(s) 113
HaeIII GGCC 6 cut(s) 68, 641, 743, 955, 1115, 1196
HapII CCGG 4 cut(s) 12, 32, 44, 237
HgaI GACGC 1 cut(s) 44
HhaI GCGC 4 cut(s) 43, 177, 757, 1239
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 4 cut(s) 742, 755, 853, 1231
Hin6I GCGC 4 cut(s) 41, 175, 755, 1237
HinP1I GCGC 4 cut(s) 41, 175, 755, 1237
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HinfI GANTC 6 cut(s) 15, 145, 464, 559, 809, 1001
HpaII CCGG 4 cut(s) 12, 32, 44, 237
HphI GGTGA 1 cut(s) 263
Hpy166II GTNNAC 2 cut(s) 436, 1162
Hpy188I TCNGA 3 cut(s) 682, 725, 794
Hpy8I GTNNAC 2 cut(s) 436, 1162
HpyAV CCTTC 5 cut(s) 299, 701, 1105, 1149, 1204
HpyCH4III ACNGT 4 cut(s) 552, 656, 788, 833
HpyCH4IV ACGT 3 cut(s) 53, 393, 1164
HpyCH4V TGCA 7 cut(s) 220, 841, 1076, 1094, 1103, 1122, 1142
HpyF10VI GCNNNNNNNGC 4 cut(s) 181, 232, 850, 1100
HpyF3I CTNAG 5 cut(s) 89, 171, 206, 573, 950
HpySE526I ACGT 3 cut(s) 53, 393, 1164
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 4 cut(s) 742, 755, 853, 1231
HspAI GCGC 4 cut(s) 41, 175, 755, 1237
Kzo9I GATC 9 cut(s) 21, 239, 351, 424, 493, 710, 794, 897, 937
LmnI GCTCC 3 cut(s) 62, 132, 232
Lsp1109I GCAGC 4 cut(s) 210, 232, 576, 853
LweI GCATC 4 cut(s) 550, 896, 1038, 1103
MaeI CTAG 3 cut(s) 630, 714, 993
MaeII ACGT 3 cut(s) 53, 393, 1164
MaeIII GTNAC 1 cut(s) 1165
MalI GATC 9 cut(s) 23, 241, 353, 426, 495, 712, 796, 899, 939
MboI GATC 9 cut(s) 21, 239, 351, 424, 493, 710, 794, 897, 937
MflI RGATCY 2 cut(s) 710, 794
MhlI GDGCHC 3 cut(s) 59, 186, 237
MlsI TGGCCA 2 cut(s) 743, 1196
MluCI AATT 6 cut(s) 187, 534, 963, 1023, 1042, 1056
MluNI TGGCCA 2 cut(s) 743, 1196
MlyI GAGTC 4 cut(s) 24, 139, 568, 1010
MmeI TCCRAC 1 cut(s) 130
Mox20I TGGCCA 2 cut(s) 743, 1196
MroXI GAANNNNTTC 1 cut(s) 456
MscI TGGCCA 2 cut(s) 743, 1196
MseI TTAA 4 cut(s) 261, 291, 537, 908
MslI CAYNNNNRTG 1 cut(s) 770
Msp20I TGGCCA 2 cut(s) 743, 1196
MspA1I CMGCKG 2 cut(s) 223, 844
MspI CCGG 4 cut(s) 12, 32, 44, 237
MspR9I CCNGG 6 cut(s) 32, 131, 237, 606, 745, 957
Mva1269I GAATGC 1 cut(s) 207
MvaI CCWGG 4 cut(s) 131, 606, 745, 957
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 4 cut(s) 181, 232, 850, 1100
NciI CCSGG 2 cut(s) 32, 237
NdeII GATC 9 cut(s) 21, 239, 351, 424, 493, 710, 794, 897, 937
NlaIII CATG 4 cut(s) 742, 755, 853, 1231
NlaIV GGNNCC 1 cut(s) 234
NsbI TGCGCA 1 cut(s) 1238
NspI RCATGY 2 cut(s) 755, 1231
PceI AGGCCT 2 cut(s) 68, 955
PciI ACATGT 1 cut(s) 1227
PctI GAATGC 1 cut(s) 207
PdmI GAANNNNTTC 1 cut(s) 456
PfeI GAWTC 2 cut(s) 464, 809
PkrI GCNGC 7 cut(s) 7, 10, 40, 222, 225, 591, 843
PleI GAGTC 4 cut(s) 23, 139, 567, 1009
PpsI GAGTC 4 cut(s) 23, 139, 567, 1009
Ppu21I YACGTR 2 cut(s) 394, 1165
PscI ACATGT 1 cut(s) 1227
Psp124BI GAGCTC 1 cut(s) 186
Psp6I CCWGG 4 cut(s) 129, 604, 743, 955
PspGI CCWGG 4 cut(s) 129, 604, 743, 955
PspN4I GGNNCC 1 cut(s) 234
PspPI GGNCC 5 cut(s) 133, 599, 640, 688, 846
PstI CTGCAG 1 cut(s) 222
PstNI CAGNNNCTG 1 cut(s) 1130
PsuI RGATCY 2 cut(s) 710, 794
PvuII CAGCTG 1 cut(s) 223
RsaI GTAC 3 cut(s) 98, 658, 698
RsaNI GTAC 3 cut(s) 97, 657, 697
RseI CAYNNNNRTG 1 cut(s) 770
SacI GAGCTC 1 cut(s) 186
SaqAI TTAA 4 cut(s) 261, 291, 537, 908
SatI GCNGC 7 cut(s) 6, 9, 39, 221, 224, 590, 842
Sau3AI GATC 9 cut(s) 21, 239, 351, 424, 493, 710, 794, 897, 937
Sau96I GGNCC 5 cut(s) 133, 599, 640, 688, 846
ScaI AGTACT 1 cut(s) 98
SchI GAGTC 4 cut(s) 24, 139, 568, 1010
ScrFI CCNGG 6 cut(s) 32, 131, 237, 606, 745, 957
SduI GDGCHC 3 cut(s) 59, 186, 237
SfaNI GCATC 4 cut(s) 550, 896, 1038, 1103
SfcI CTRYAG 1 cut(s) 218
SinI GGWCC 4 cut(s) 133, 599, 688, 846
SmiMI CAYNNNNRTG 1 cut(s) 770
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
Sse9I AATT 6 cut(s) 187, 534, 963, 1023, 1042, 1056
SseBI AGGCCT 2 cut(s) 68, 955
SsiI CCGC 5 cut(s) 9, 39, 60, 136, 844
SspMI CTAG 3 cut(s) 630, 714, 993
SstI GAGCTC 1 cut(s) 186
StuI AGGCCT 2 cut(s) 68, 955
StyD4I CCNGG 6 cut(s) 30, 129, 235, 604, 743, 955
StyI CCWWGG 2 cut(s) 63, 1197
TaaI ACNGT 4 cut(s) 552, 656, 788, 833
TaiI ACGT 3 cut(s) 56, 396, 1167
TaqI TCGA 4 cut(s) 18, 165, 555, 783
TasI AATT 6 cut(s) 187, 534, 963, 1023, 1042, 1056
TatI WGTACW 3 cut(s) 96, 656, 696
TauI GCSGC 2 cut(s) 11, 41
TfiI GAWTC 2 cut(s) 464, 809
Tru1I TTAA 4 cut(s) 261, 291, 537, 908
Tru9I TTAA 4 cut(s) 261, 291, 537, 908
TscAI CASTG 3 cut(s) 616, 838, 1197
TseI GCWGC 5 cut(s) 5, 220, 223, 589, 841
TspDTI ATGAA 8 cut(s) 287, 458, 652, 792, 1016, 1055, 1100, 1220
TspGWI ACGGA 1 cut(s) 368
TspRI CASTG 3 cut(s) 616, 838, 1197
VpaK11BI GGWCC 4 cut(s) 133, 599, 688, 846
XapI RAATTY 2 cut(s) 1023, 1042
XbaI TCTAGA 1 cut(s) 713
XceI RCATGY 2 cut(s) 755, 1231
XcmI CCANNNNNNNNNTGG 1 cut(s) 698
XmnI GAANNNNTTC 1 cut(s) 456
XspI CTAG 3 cut(s) 630, 714, 993
ZrmI AGTACT 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.