Rroxscaffold_2G00151910

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012336
Physical Location & Seq
Forward (+)
89044972 .. 89048797
3826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00151910.1

Sequence Viewer

Length: 1248 bp
ATGGCTGCCGTCGGCGTCGAGACCAAGCCCGTCGAGGCCGTGCCGGAGGACACCTGCTCCGCCAAGCTCGCCGCCTCCAAACTCGGCGAGGGGCTCCGCCCTTACTATCTCCAACACATTCACGAGCTCCAGCTCCAGGTCCCGCCAGAAGACCCACAATCTCAATCGACTCGAGGCTCAGCGGAACGAGCTCAATTCCAGAGGGAAGAACTACAACTTCTTCAGGAGCCTGGGTCCTATGTCGGCGAGGTTGTCAACGTCATGGGAAAGAACAAGGTTTTGGTTAAGGTTCATCCGGAAGGGAAGTATGTGGTTGATATTGATAAGAACATTGATATCACAAAGATCACACCATCTACAAGAGTGGCTCTACGCAATGACAGCTATGTTCTTCATTTAGTGCTGCCAAGCAAAGTAGATCCTCTCGTCAATCTGATGAAAGTGGAAAAGGTTCCAGATTCTACATATGATATGATTGGTGGTCTGGATCAGCAGATTAAAGAAATCAAAGAGGTCATTGAACTTCCAATTAAACATCCTGAATTGTTTGAAAGTCTTGGAATAGCTCAGCCAAAGGGAGTCCTGCTTTATGGTCCCCCCGGCACTGGCAAAACCTTGTTGGCTAGGGCTGTGGCTCATCACACTGACTGTACTTTCATCAGGGTTTCTGGTTCAGAGTTGGTTCAGAAATACATTGGGGAAGGTTCCCGAATGGTTAGAGAACTTTTCGTAATGGCCAGGGAACATGCTCCATCTATCATTTTTATGGATGAAATCGATAGTATTGGATCTGCTCGCATGGAATCTGGCAGTGGCAATGGGGACAGCGAGGTGCAGAGGACTATGCTTGAGCTTCTTAACCAACTGGATGGATTTGAGGCCTCAAACAAAATCAAGGTTCTGATGGCTACAAATCGTATTGATATTCTGGATCAAGCCCTGCTGAGGCCAGGACGCATAGACAGAAAGATCGAATTTCCCAACCCTAACGAAGAGTCTCGCTGGGACATTCTGAAAATCCATTCAAGAAGAATGAATTTAATGCGTGGAATTGACCTGAAGAAGATTGCGGAGAAAATGAACGGCGCCTCTGGCGCTGAGTTGAAGGCTGTTTGCACTGAAGCAGGAATGTTTGCACTGAGGGAGAGGAGAGTGCATGTAACCCAAGAAGACTTTGAGATGGCAGTTGCCAAGGTTATGAAGAAGGAGACGGAGAAGAATATGTCCCTGCGTAAGCTGTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0030162 GO:0030163 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

415

Amino Acids

46.44

Weight (kDa)

6.59

Isoelectric Point (pI)

45.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 81 - 136 9.6e-13 Proteasomal ATPase OB C-terminal domain
AAA PF00004 194 - 327 5.3e-44 ATPase family associated with various cellular activities (AAA)
AAA_2 PF07724 194 - 287 9.9e-06 AAA domain (Cdc48 subfamily)
AAA_lid_PRS2_C PF23902 332 - 401 9.8e-06 PRS2 AAA+ lid C-terminal domain
AAA_lid_3 PF17862 351 - 393 1.5e-14 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012336)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19990 AT5G19990 AT5G19990 AT5G20000
fragaria_vesca FvH4_1g03980
malus_domestica MD02G1226600.v1.1 MD07G1087400.v1.1
prunus_persica Prupe.7G237100_v2.0.a1
pyrus_communis pycom02g19560
rosa_chinensis RchiOBHm_Chr2g0089461
rosa_laevigata RLG00000016031
rosa_multiflora Rmu_sc0000197.1_g000006
rosa_roxburghii Rroxscaffold_2G00151910 Rroxscaffold_6G00403280
rosa_rugosa Rorug02G0001000
rosa_samantha Rh2AG046200 Rh2CG046600 Rh2DG046200
rosa_wichuraiana Rw2G003640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 62
Acc36I ACCTGC 1 cut(s) 62
AccB1I GGYRCC 1 cut(s) 1085
AccIII TCCGGA 1 cut(s) 295
AciI CCGC 6 cut(s) 60, 72, 97, 143, 182, 1070
AclWI GGATC 4 cut(s) 413, 495, 796, 939
AcoI YGGCCR 1 cut(s) 735
AcsI RAATTY 2 cut(s) 974, 1036
AcuI CTGAAG 3 cut(s) 206, 1079, 1140
AcyI GRCGYC 2 cut(s) 15, 1086
AfaI GTAC 1 cut(s) 652
AfiI CCNNNNNNNGG 3 cut(s) 136, 605, 945
AgsI TTSAA 4 cut(s) 521, 551, 1026, 1105
AjnI CCWGG 4 cut(s) 135, 229, 737, 949
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AluBI AGCT 8 cut(s) 67, 127, 133, 191, 384, 566, 853, 1237
AluI AGCT 8 cut(s) 67, 127, 133, 191, 384, 566, 853, 1237
Alw21I GWGCWC 2 cut(s) 129, 193
Alw26I GTCTC 3 cut(s) 14, 1002, 1202
AlwI GGATC 4 cut(s) 413, 495, 796, 939
Ama87I CYCGRG 1 cut(s) 171
Aor13HI TCCGGA 1 cut(s) 295
AoxI GGCC 4 cut(s) 36, 735, 879, 947
ApeKI GCWGC 2 cut(s) 5, 403
ApoI RAATTY 2 cut(s) 974, 1036
Asp700I GAANNNNTTC 1 cut(s) 450
AspLEI GCGC 2 cut(s) 1088, 1097
AspS9I GGNCC 3 cut(s) 139, 234, 593
AsuC2I CCSGG 1 cut(s) 600
AvaI CYCGRG 1 cut(s) 171
AvaII GGWCC 3 cut(s) 139, 234, 593
BalI TGGCCA 1 cut(s) 737
BanI GGYRCC 1 cut(s) 1085
BanII GRGCYC 3 cut(s) 96, 129, 193
BauI CACGAG 1 cut(s) 122
BbsI GAAGAC 2 cut(s) 156, 1176
Bbv12I GWGCWC 2 cut(s) 129, 193
BbvCI CCTCAGC 1 cut(s) 944
BbvI GCAGC 1 cut(s) 390
BccI CCATC 5 cut(s) 361, 760, 863, 898, 1174
BceAI ACGGC 2 cut(s) 23, 1099
BciT130I CCWGG 4 cut(s) 137, 231, 739, 951
BcnI CCSGG 1 cut(s) 600
BcoDI GTCTC 3 cut(s) 14, 1002, 1202
BfaI CTAG 1 cut(s) 624
BfoI RGCGCY 2 cut(s) 1089, 1098
BfuAI ACCTGC 1 cut(s) 62
BisI GCNGC 3 cut(s) 6, 72, 404
BlpI GCTNAGC 2 cut(s) 178, 567
BlsI GCNGC 3 cut(s) 7, 73, 405
Bme1390I CCNGG 5 cut(s) 137, 231, 600, 739, 951
Bme18I GGWCC 3 cut(s) 139, 234, 593
BmeT110I CYCGRG 1 cut(s) 171
BmgT120I GGNCC 3 cut(s) 139, 234, 593
BmiI GGNNCC 8 cut(s) 95, 141, 228, 235, 453, 595, 706, 1087
BmrFI CCNGG 5 cut(s) 137, 231, 600, 739, 951
BpiI GAAGAC 2 cut(s) 156, 1176
BpmI CTGGAG 2 cut(s) 113, 119
Bpu10I CCTNAGC 1 cut(s) 944
Bpu1102I GCTNAGC 2 cut(s) 178, 567
BpuEI CTTGAG 1 cut(s) 869
BpuMI CCSGG 1 cut(s) 600
Bsa29I ATCGAT 1 cut(s) 777
BsaHI GRCGYC 2 cut(s) 15, 1086
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 4 cut(s) 230, 598, 738, 1191
BsaWI WCCGGW 1 cut(s) 295
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 218, 248
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 605, 945
Bse1I ACTGG 2 cut(s) 610, 870
Bse3DI GCAATG 2 cut(s) 382, 823
BseAI TCCGGA 1 cut(s) 295
BseBI CCWGG 4 cut(s) 137, 231, 739, 951
BseCI ATCGAT 1 cut(s) 777
BseDI CCNNGG 4 cut(s) 230, 598, 738, 1191
BseGI GGATG 4 cut(s) 292, 535, 775, 874
BseLI CCNNNNNNNGG 3 cut(s) 136, 605, 945
BseMI GCAATG 2 cut(s) 382, 823
BseMII CTCAG 5 cut(s) 192, 581, 935, 1089, 1130
BseNI ACTGG 2 cut(s) 610, 870
BseRI GAGGAG 1 cut(s) 1162
BseXI GCAGC 1 cut(s) 390
BseYI CCCAGC 1 cut(s) 1002
BsgI GTGCAG 1 cut(s) 854
BshFI GGCC 4 cut(s) 38, 737, 881, 949
BshNI GGYRCC 1 cut(s) 1085
BshVI ATCGAT 1 cut(s) 777
BsiHKAI GWGCWC 2 cut(s) 129, 193
BsiHKCI CYCGRG 1 cut(s) 171
BsiSI CCGG 3 cut(s) 44, 296, 600
BslFI GGGAC 5 cut(s) 125, 579, 836, 1019, 1210
BslI CCNNNNNNNGG 3 cut(s) 136, 605, 945
BsmAI GTCTC 3 cut(s) 14, 1002, 1202
BsmBI CGTCTC 1 cut(s) 1202
BsmFI GGGAC 5 cut(s) 125, 579, 836, 1019, 1210
BsnI GGCC 4 cut(s) 38, 737, 881, 949
Bso31I GGTCTC 1 cut(s) 14
BsoBI CYCGRG 1 cut(s) 171
Bsp1286I GDGCHC 3 cut(s) 96, 129, 193
Bsp13I TCCGGA 1 cut(s) 295
Bsp143I GATC 6 cut(s) 345, 418, 487, 788, 931, 969
Bsp1720I GCTNAGC 2 cut(s) 178, 567
BspACI CCGC 6 cut(s) 60, 72, 97, 143, 182, 1070
BspANI GGCC 4 cut(s) 38, 737, 881, 949
BspCNI CTCAG 5 cut(s) 191, 580, 936, 1090, 1131
BspDI ATCGAT 1 cut(s) 777
BspEI TCCGGA 1 cut(s) 295
BspLI GGNNCC 8 cut(s) 95, 141, 228, 235, 453, 595, 706, 1087
BspMI ACCTGC 1 cut(s) 62
BspPI GGATC 4 cut(s) 413, 495, 796, 939
BspT107I GGYRCC 1 cut(s) 1085
BspTNI GGTCTC 1 cut(s) 14
BsrDI GCAATG 2 cut(s) 382, 823
BsrI ACTGG 2 cut(s) 610, 870
BssECI CCNNGG 4 cut(s) 230, 598, 738, 1191
BssMI GATC 6 cut(s) 345, 418, 487, 788, 931, 969
BssNI GRCGYC 2 cut(s) 15, 1086
BssSI CACGAG 1 cut(s) 122
BssT1I CCWWGG 1 cut(s) 1191
Bst2BI CACGAG 1 cut(s) 122
Bst2UI CCWGG 4 cut(s) 137, 231, 739, 951
Bst4CI ACNGT 1 cut(s) 650
Bst6I CTCTTC 1 cut(s) 987
BstACI GRCGYC 2 cut(s) 15, 1086
BstC8I GCNNGC 2 cut(s) 69, 796
BstDEI CTNAG 5 cut(s) 178, 567, 944, 1098, 1139
BstF5I GGATG 4 cut(s) 292, 535, 775, 874
BstH2I RGCGCY 2 cut(s) 1089, 1098
BstHHI GCGC 2 cut(s) 1088, 1097
BstKTI GATC 6 cut(s) 348, 421, 490, 791, 934, 972
BstMAI GTCTC 3 cut(s) 14, 1002, 1202
BstMBI GATC 6 cut(s) 345, 418, 487, 788, 931, 969
BstMWI GCNNNNNNNGC 5 cut(s) 68, 188, 381, 1092, 1094
BstNI CCWGG 4 cut(s) 137, 231, 739, 951
BstNSI RCATGY 2 cut(s) 749, 1160
BstSCI CCNGG 5 cut(s) 135, 229, 598, 737, 949
BstV1I GCAGC 1 cut(s) 390
BstV2I GAAGAC 2 cut(s) 156, 1176
BstX2I RGATCY 2 cut(s) 418, 788
BstXI CCANNNNNNTGG 1 cut(s) 869
BstYI RGATCY 2 cut(s) 418, 788
Bsu15I ATCGAT 1 cut(s) 777
BsuRI GGCC 4 cut(s) 38, 737, 881, 949
BsuTUI ATCGAT 1 cut(s) 777
BtsCI GGATG 4 cut(s) 292, 535, 775, 874
BtsI GCAGTG 1 cut(s) 817
BtsIMutI CAGTG 5 cut(s) 603, 642, 817, 1116, 1136
BveI ACCTGC 1 cut(s) 62
Cac8I GCNNGC 2 cut(s) 69, 796
CfoI GCGC 2 cut(s) 1088, 1097
Cfr13I GGNCC 3 cut(s) 139, 234, 593
ClaI ATCGAT 1 cut(s) 777
CseI GACGC 2 cut(s) 4, 963
Csp6I GTAC 1 cut(s) 651
CviAII CATG 4 cut(s) 262, 746, 799, 1157
CviQI GTAC 1 cut(s) 651
DdeI CTNAG 5 cut(s) 178, 567, 944, 1098, 1139
DinI GGCGCC 1 cut(s) 1087
DpnI GATC 6 cut(s) 347, 420, 489, 790, 933, 971
DpnII GATC 6 cut(s) 345, 418, 487, 788, 931, 969
EaeI YGGCCR 1 cut(s) 735
Eam1104I CTCTTC 1 cut(s) 987
EarI CTCTTC 1 cut(s) 987
EciI GGCGGA 2 cut(s) 49, 86
Ecl136II GAGCTC 2 cut(s) 127, 191
Eco130I CCWWGG 1 cut(s) 1191
Eco147I AGGCCT 1 cut(s) 881
Eco24I GRGCYC 3 cut(s) 96, 129, 193
Eco31I GGTCTC 1 cut(s) 14
Eco32I GATATC 1 cut(s) 337
Eco47I GGWCC 3 cut(s) 139, 234, 593
Eco53kI GAGCTC 2 cut(s) 127, 191
Eco57I CTGAAG 3 cut(s) 206, 1079, 1140
Eco88I CYCGRG 1 cut(s) 171
EcoICRI GAGCTC 2 cut(s) 127, 191
EcoO109I RGGNCCY 2 cut(s) 139, 234
EcoRII CCWGG 4 cut(s) 135, 229, 737, 949
EcoRV GATATC 1 cut(s) 337
EcoT14I CCWWGG 1 cut(s) 1191
EcoT38I GRGCYC 3 cut(s) 96, 129, 193
EgeI GGCGCC 1 cut(s) 1087
EheI GGCGCC 1 cut(s) 1087
ErhI CCWWGG 1 cut(s) 1191
Esp3I CGTCTC 1 cut(s) 1202
FaeI CATG 4 cut(s) 265, 749, 802, 1160
FaqI GGGAC 5 cut(s) 125, 579, 836, 1019, 1210
FatI CATG 4 cut(s) 261, 745, 798, 1156
FauI CCCGC 1 cut(s) 150
FauNDI CATATG 1 cut(s) 466
Fnu4HI GCNGC 3 cut(s) 6, 72, 404
FokI GGATG 4 cut(s) 279, 522, 782, 881
FriOI GRGCYC 3 cut(s) 96, 129, 193
Fsp4HI GCNGC 3 cut(s) 6, 72, 404
FspBI CTAG 1 cut(s) 624
GlaI GCGC 2 cut(s) 1087, 1096
GluI GCNGC 3 cut(s) 6, 72, 404
GsaI CCCAGC 1 cut(s) 1006
GsuI CTGGAG 2 cut(s) 113, 119
HaeII RGCGCY 2 cut(s) 1089, 1098
HaeIII GGCC 4 cut(s) 38, 737, 881, 949
HapII CCGG 3 cut(s) 44, 296, 600
HgaI GACGC 2 cut(s) 4, 963
HhaI GCGC 2 cut(s) 1088, 1097
Hin1I GRCGYC 2 cut(s) 15, 1086
Hin1II CATG 4 cut(s) 265, 749, 802, 1160
Hin6I GCGC 2 cut(s) 1086, 1095
HinP1I GCGC 2 cut(s) 1086, 1095
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HinfI GANTC 5 cut(s) 169, 458, 579, 803, 995
HpaII CCGG 3 cut(s) 44, 296, 600
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 5 cut(s) 435, 676, 687, 903, 1014
Hpy8I GTNNAC 1 cut(s) 256
Hpy99I CGWCG 3 cut(s) 14, 20, 35
HpyAV CCTTC 4 cut(s) 293, 695, 1099, 1198
HpyCH4III ACNGT 1 cut(s) 650
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 4 cut(s) 835, 1116, 1136, 1156
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 188, 381, 1092, 1094
HpyF3I CTNAG 5 cut(s) 178, 567, 944, 1098, 1139
HpySE526I ACGT 1 cut(s) 258
Hsp92I GRCGYC 2 cut(s) 15, 1086
Hsp92II CATG 4 cut(s) 265, 749, 802, 1160
HspAI GCGC 2 cut(s) 1086, 1095
KasI GGCGCC 1 cut(s) 1085
Kpn2I TCCGGA 1 cut(s) 295
Kzo9I GATC 6 cut(s) 345, 418, 487, 788, 931, 969
LmnI GCTCC 6 cut(s) 62, 99, 132, 138, 226, 754
Lsp1109I GCAGC 1 cut(s) 390
MaeI CTAG 1 cut(s) 624
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 1 cut(s) 1159
MalI GATC 6 cut(s) 347, 420, 489, 790, 933, 971
MboI GATC 6 cut(s) 345, 418, 487, 788, 931, 969
MflI RGATCY 2 cut(s) 418, 788
MhlI GDGCHC 3 cut(s) 96, 129, 193
MlsI TGGCCA 1 cut(s) 737
MluCI AATT 6 cut(s) 194, 528, 542, 974, 1036, 1050
MluNI TGGCCA 1 cut(s) 737
Mly113I GGCGCC 1 cut(s) 1086
MlyI GAGTC 3 cut(s) 163, 588, 1004
MmeI TCCRAC 1 cut(s) 136
Mox20I TGGCCA 1 cut(s) 737
MroI TCCGGA 1 cut(s) 295
MroXI GAANNNNTTC 1 cut(s) 450
MscI TGGCCA 1 cut(s) 737
MseI TTAA 5 cut(s) 285, 498, 531, 858, 1040
MslI CAYNNNNRTG 1 cut(s) 764
Msp20I TGGCCA 1 cut(s) 737
MspA1I CMGCKG 1 cut(s) 182
MspI CCGG 3 cut(s) 44, 296, 600
MspR9I CCNGG 5 cut(s) 137, 231, 600, 739, 951
MvaI CCWGG 4 cut(s) 137, 231, 739, 951
MwoI GCNNNNNNNGC 5 cut(s) 68, 188, 381, 1092, 1094
NarI GGCGCC 1 cut(s) 1086
NciI CCSGG 1 cut(s) 600
NdeI CATATG 1 cut(s) 466
NdeII GATC 6 cut(s) 345, 418, 487, 788, 931, 969
NlaIII CATG 4 cut(s) 265, 749, 802, 1160
NlaIV GGNNCC 8 cut(s) 95, 141, 228, 235, 453, 595, 706, 1087
NmeAIII GCCGAG 1 cut(s) 63
NspI RCATGY 2 cut(s) 749, 1160
PaeR7I CTCGAG 1 cut(s) 171
PaqCI CACCTGC 1 cut(s) 62
PceI AGGCCT 1 cut(s) 881
PdmI GAANNNNTTC 1 cut(s) 450
PfeI GAWTC 2 cut(s) 458, 803
PkrI GCNGC 3 cut(s) 7, 73, 405
PleI GAGTC 3 cut(s) 163, 587, 1003
PluTI GGCGCC 1 cut(s) 1089
PpsI GAGTC 3 cut(s) 163, 587, 1003
PpuMI RGGWCCY 2 cut(s) 139, 234
Psp124BI GAGCTC 2 cut(s) 129, 193
Psp5II RGGWCCY 2 cut(s) 139, 234
Psp6I CCWGG 4 cut(s) 135, 229, 737, 949
PspFI CCCAGC 1 cut(s) 1002
PspGI CCWGG 4 cut(s) 135, 229, 737, 949
PspN4I GGNNCC 8 cut(s) 95, 141, 228, 235, 453, 595, 706, 1087
PspPI GGNCC 3 cut(s) 139, 234, 593
PspPPI RGGWCCY 2 cut(s) 139, 234
PspXI VCTCGAGB 1 cut(s) 171
PsuI RGATCY 2 cut(s) 418, 788
RsaI GTAC 1 cut(s) 652
RsaNI GTAC 1 cut(s) 651
RseI CAYNNNNRTG 1 cut(s) 764
SacI GAGCTC 2 cut(s) 129, 193
SaqAI TTAA 5 cut(s) 285, 498, 531, 858, 1040
SatI GCNGC 3 cut(s) 6, 72, 404
Sau3AI GATC 6 cut(s) 345, 418, 487, 788, 931, 969
Sau96I GGNCC 3 cut(s) 139, 234, 593
SchI GAGTC 3 cut(s) 163, 588, 1004
ScrFI CCNGG 5 cut(s) 137, 231, 600, 739, 951
SduI GDGCHC 3 cut(s) 96, 129, 193
SfoI GGCGCC 1 cut(s) 1087
Sfr274I CTCGAG 1 cut(s) 171
SinI GGWCC 3 cut(s) 139, 234, 593
SlaI CTCGAG 1 cut(s) 171
SmiMI CAYNNNNRTG 1 cut(s) 764
SmlI CTYRAG 2 cut(s) 171, 848
SmoI CTYRAG 2 cut(s) 171, 848
Sse9I AATT 6 cut(s) 194, 528, 542, 974, 1036, 1050
SseBI AGGCCT 1 cut(s) 881
SsiI CCGC 6 cut(s) 60, 72, 97, 143, 182, 1070
SspDI GGCGCC 1 cut(s) 1085
SspMI CTAG 1 cut(s) 624
SstI GAGCTC 2 cut(s) 129, 193
StuI AGGCCT 1 cut(s) 881
StyD4I CCNGG 5 cut(s) 135, 229, 598, 737, 949
StyI CCWWGG 1 cut(s) 1191
TaaI ACNGT 1 cut(s) 650
TaiI ACGT 1 cut(s) 261
TaqI TCGA 6 cut(s) 18, 33, 167, 172, 777, 972
TasI AATT 6 cut(s) 194, 528, 542, 974, 1036, 1050
TatI WGTACW 1 cut(s) 650
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 2 cut(s) 458, 803
Tru1I TTAA 5 cut(s) 285, 498, 531, 858, 1040
Tru9I TTAA 5 cut(s) 285, 498, 531, 858, 1040
TscAI CASTG 5 cut(s) 610, 649, 817, 1123, 1143
TseI GCWGC 2 cut(s) 5, 403
TspDTI ATGAA 8 cut(s) 281, 383, 452, 646, 786, 1049, 1094, 1214
TspGWI ACGGA 1 cut(s) 1226
TspRI CASTG 5 cut(s) 610, 649, 817, 1123, 1143
VpaK11BI GGWCC 3 cut(s) 139, 234, 593
XapI RAATTY 2 cut(s) 974, 1036
XceI RCATGY 2 cut(s) 749, 1160
XhoI CTCGAG 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 450
XspI CTAG 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.