MD02G1227000.v1.1

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0018483
Physical Location & Seq
Forward (+)
26761985 .. 26765959
3975 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1227000.v1.1.491

Sequence Viewer

Length: 1155 bp
ATGTACCTCTCCAACCGGTTGTTCTCTGGGGCTCAAAATGTACCACTTTGGAGGACAGACGGAGTTTCTCAAGACCATAAAAATGGTAGTGATTTAGAGGAAAATGTTGGTGATGGGTCTCAAGACCATGAAAATGGTGGTGATATAGATTTAAATGTTGATGATGATCATGTTGGTGTAGAGGAAAATATTGAATCTCCCGAACCTACATTCCATTTAAACATTTATGATCCAAGAATTTGGGATGATCTTAATGCAGAAATGAGAGCCTTATTTGTAGAAAATGGACCAATTCGAGAAACTAATCTCACTTATCCTAAGGACAAACTCTCTAGAAAATTTTCCTCACACTACTATGATCGAAAATTACCAACGGGTGAAATTTATGATAGGAAATGGCTCGTGTACTCAAAAGAGTTGGATAAAATCTTTTGCTTTTGTTGTAAATTGTTTAAAACAATTACCTCAAAAAGTGAGTTAGCAAAAGATGGAATTAGTGATTGGAGACATCTTGGTGTGAAGCTTGATCAACATGAAAAGACTGTGATTCACCCATTTGCGGAGCACATAGTATATTCCTTGCTCTTCTCAATACCAATGCTAGCAACTGTGTTCATGGGAACAGTTTCTGTCTTATCCTGTGTTGGCTATACTTATATTGAGTTCATGAATAACATGGGACACTGCAATTTTGAGCTCATTCCAAACTATCTCTTCTCTCTCTTTCCTCCTCTCAAGTATCTTATGTATACCTCTTCTTTTCACTTTTTGCATCACACACAATTCCGAACCAATTACTGTCTCTTCATGCCATTCTACGATTACGTATATGGCACCGTGGACAATTCTGCTGATTCACTCTATGAAACTTCACTCAAGAGAGAGGAGGAATCGCCAGATATCCTTCATCTAACCCATCTAACAACACCGGAATCCATCTATCATCTACCGCTAGGGTTTGCTTCCTTGGCCTCTATACCCCACACATCAAAATGGTACCTGTGGTTGATGTGGCCTGTGACATTGTGGTACATGGTGTTAACTCGGATCTGTGGTCGTACATTTGTGGTTGAGAGGCAGCGTTTTAACAAGCTCGGATTACAAACTTGGGTTATACCCAAATACACTTTGCAATACTACTTTCGATTCCAGTAA

Protein Analysis

385

Amino Acids

45.11

Weight (kDa)

5.77

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 181 - 279 3.1e-09 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 996
AccB1I GGYRCC 2 cut(s) 833, 996
AccI GTMKAC 1 cut(s) 749
AciI CCGC 2 cut(s) 560, 950
AclWI GGATC 2 cut(s) 224, 1055
AcsI RAATTY 3 cut(s) 237, 338, 381
AfaI GTAC 6 cut(s) 5, 42, 407, 998, 1031, 1060
AfiI CCNNNNNNNGG 1 cut(s) 559
AgeI ACCGGT 1 cut(s) 15
AgsI TTSAA 1 cut(s) 194
AjuI GAANNNNNNNTTGG 3 cut(s) 37, 484, 516
AloI GAACNNNNNNTCC 2 cut(s) 195, 227
AluBI AGCT 3 cut(s) 523, 697, 1093
AluI AGCT 3 cut(s) 523, 697, 1093
Alw21I GWGCWC 2 cut(s) 567, 699
Alw26I GTCTC 3 cut(s) 123, 499, 806
AlwI GGATC 2 cut(s) 224, 1055
AlwNI CAGNNNCTG 1 cut(s) 629
AoxI GGCC 2 cut(s) 969, 1013
ApeKI GCWGC 1 cut(s) 1078
ApoI RAATTY 3 cut(s) 237, 338, 381
AsiGI ACCGGT 1 cut(s) 15
Asp700I GAANNNNTTC 2 cut(s) 340, 625
Asp718I GGTACC 1 cut(s) 996
AspS9I GGNCC 1 cut(s) 287
AsuHPI GGTGA 4 cut(s) 122, 152, 389, 542
AsuNHI GCTAGC 1 cut(s) 601
AvaII GGWCC 1 cut(s) 287
AxyI CCTNAGG 1 cut(s) 318
BanI GGYRCC 2 cut(s) 833, 996
BanII GRGCYC 2 cut(s) 34, 699
BauI CACGAG 1 cut(s) 401
Bbv12I GWGCWC 2 cut(s) 567, 699
BbvI GCAGC 1 cut(s) 1090
BccI CCATC 4 cut(s) 107, 482, 924, 944
BclI TGATCA 2 cut(s) 166, 526
BcoDI GTCTC 3 cut(s) 123, 499, 806
BfaI CTAG 3 cut(s) 333, 602, 953
BisI GCNGC 1 cut(s) 1079
BlsI GCNGC 1 cut(s) 1080
Bme18I GGWCC 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 287
BmiI GGNNCC 2 cut(s) 835, 998
BmsI GCATC 1 cut(s) 781
BmtI GCTAGC 1 cut(s) 605
BpuEI CTTGAG 4 cut(s) 54, 105, 719, 860
BsaAI YACGTR 1 cut(s) 826
BsaBI GATNNNNATC 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 123
BsaJI CCNNGG 2 cut(s) 837, 966
BsaWI WCCGGW 2 cut(s) 15, 928
Bsc4I CCNNNNNNNGG 1 cut(s) 559
Bse118I RCCGGY 1 cut(s) 15
Bse1I ACTGG 1 cut(s) 1150
Bse21I CCTNAGG 1 cut(s) 318
Bse8I GATNNNNATC 1 cut(s) 165
BseDI CCNNGG 2 cut(s) 837, 966
BseGI GGATG 1 cut(s) 250
BseJI GATNNNNATC 1 cut(s) 165
BseLI CCNNNNNNNGG 1 cut(s) 559
BseNI ACTGG 1 cut(s) 1150
BseRI GAGGAG 2 cut(s) 720, 899
BseXI GCAGC 1 cut(s) 1090
BshFI GGCC 2 cut(s) 971, 1015
BshNI GGYRCC 2 cut(s) 833, 996
BshTI ACCGGT 1 cut(s) 15
BsiHKAI GWGCWC 2 cut(s) 567, 699
BsiSI CCGG 2 cut(s) 16, 929
BslFI GGGAC 1 cut(s) 693
BslI CCNNNNNNNGG 1 cut(s) 559
BsmAI GTCTC 3 cut(s) 123, 499, 806
BsmFI GGGAC 1 cut(s) 693
BsnI GGCC 2 cut(s) 971, 1015
Bso31I GGTCTC 1 cut(s) 123
Bsp1286I GDGCHC 3 cut(s) 34, 567, 699
Bsp143I GATC 6 cut(s) 166, 229, 247, 358, 526, 1047
BspACI CCGC 2 cut(s) 560, 950
BspANI GGCC 2 cut(s) 971, 1015
BspHI TCATGA 1 cut(s) 666
BspLI GGNNCC 2 cut(s) 835, 998
BspOI GCTAGC 1 cut(s) 605
BspPI GGATC 2 cut(s) 224, 1055
BspQI GCTCTTC 1 cut(s) 590
BspT107I GGYRCC 2 cut(s) 833, 996
BspTNI GGTCTC 1 cut(s) 123
BsrFI RCCGGY 1 cut(s) 15
BsrI ACTGG 1 cut(s) 1150
BssAI RCCGGY 1 cut(s) 15
BssECI CCNNGG 2 cut(s) 837, 966
BssMI GATC 6 cut(s) 166, 229, 247, 358, 526, 1047
BssNAI GTATAC 1 cut(s) 750
BssSI CACGAG 1 cut(s) 401
BssT1I CCWWGG 1 cut(s) 966
Bst1107I GTATAC 1 cut(s) 750
Bst2BI CACGAG 1 cut(s) 401
Bst4CI ACNGT 5 cut(s) 544, 610, 625, 800, 838
Bst6I CTCTTC 4 cut(s) 590, 719, 760, 809
BstBAI YACGTR 1 cut(s) 826
BstC8I GCNNGC 1 cut(s) 603
BstDEI CTNAG 1 cut(s) 318
BstDSI CCRYGG 1 cut(s) 837
BstF5I GGATG 1 cut(s) 250
BstKTI GATC 6 cut(s) 169, 232, 250, 361, 529, 1050
BstMAI GTCTC 3 cut(s) 123, 499, 806
BstMBI GATC 6 cut(s) 166, 229, 247, 358, 526, 1047
BstMWI GCNNNNNNNGC 1 cut(s) 968
BstSNI TACGTA 1 cut(s) 826
BstV1I GCAGC 1 cut(s) 1090
BstX2I RGATCY 1 cut(s) 1047
BstXI CCANNNNNNTGG 3 cut(s) 83, 134, 240
BstYI RGATCY 1 cut(s) 1047
BstZ17I GTATAC 1 cut(s) 750
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 2 cut(s) 971, 1015
BtgI CCRYGG 1 cut(s) 837
BtsCI GGATG 1 cut(s) 250
BtsI GCAGTG 1 cut(s) 682
BtsIMutI CAGTG 1 cut(s) 682
Cac8I GCNNGC 1 cut(s) 603
CaiI CAGNNNCTG 1 cut(s) 629
CciI TCATGA 1 cut(s) 666
Cfr10I RCCGGY 1 cut(s) 15
Cfr13I GGNCC 1 cut(s) 287
Csp6I GTAC 6 cut(s) 4, 41, 406, 997, 1030, 1059
CspAI ACCGGT 1 cut(s) 15
CviAII CATG 8 cut(s) 128, 170, 533, 616, 667, 676, 808, 1033
CviJI RGCY 9 cut(s) 32, 269, 400, 523, 648, 697, 971, 1015, 1093
CviKI_1 RGCY 9 cut(s) 32, 269, 400, 523, 648, 697, 971, 1015, 1093
CviQI GTAC 6 cut(s) 4, 41, 406, 997, 1030, 1059
DdeI CTNAG 1 cut(s) 318
DpnI GATC 6 cut(s) 168, 231, 249, 360, 528, 1049
DpnII GATC 6 cut(s) 166, 229, 247, 358, 526, 1047
DraI TTTAAA 3 cut(s) 153, 219, 454
Eam1104I CTCTTC 4 cut(s) 590, 719, 760, 809
EarI CTCTTC 4 cut(s) 590, 719, 760, 809
Ecl136II GAGCTC 1 cut(s) 697
Eco105I TACGTA 1 cut(s) 826
Eco130I CCWWGG 1 cut(s) 966
Eco24I GRGCYC 2 cut(s) 34, 699
Eco31I GGTCTC 1 cut(s) 123
Eco32I GATATC 1 cut(s) 901
Eco47I GGWCC 1 cut(s) 287
Eco53kI GAGCTC 1 cut(s) 697
Eco81I CCTNAGG 1 cut(s) 318
EcoICRI GAGCTC 1 cut(s) 697
EcoRV GATATC 1 cut(s) 901
EcoT14I CCWWGG 1 cut(s) 966
EcoT38I GRGCYC 2 cut(s) 34, 699
ErhI CCWWGG 1 cut(s) 966
FaeI CATG 8 cut(s) 131, 173, 536, 619, 670, 679, 811, 1036
FaqI GGGAC 1 cut(s) 693
FatI CATG 8 cut(s) 127, 169, 532, 615, 666, 675, 807, 1032
FbaI TGATCA 2 cut(s) 166, 526
FblI GTMKAC 1 cut(s) 749
Fnu4HI GCNGC 1 cut(s) 1079
FokI GGATG 1 cut(s) 257
FriOI GRGCYC 2 cut(s) 34, 699
Fsp4HI GCNGC 1 cut(s) 1079
FspBI CTAG 3 cut(s) 333, 602, 953
GluI GCNGC 1 cut(s) 1079
HaeIII GGCC 2 cut(s) 971, 1015
HapII CCGG 2 cut(s) 16, 929
Hin1II CATG 8 cut(s) 131, 173, 536, 619, 670, 679, 811, 1036
HincII GTYRAC 1 cut(s) 1041
HindII GTYRAC 1 cut(s) 1041
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 6 cut(s) 194, 547, 854, 890, 932, 1146
HpaI GTTAAC 1 cut(s) 1041
HpaII CCGG 2 cut(s) 16, 929
HphI GGTGA 4 cut(s) 122, 152, 389, 542
Hpy166II GTNNAC 4 cut(s) 406, 750, 841, 1041
Hpy188I TCNGA 3 cut(s) 788, 1047, 1097
Hpy188III TCNNGA 7 cut(s) 71, 122, 200, 296, 333, 667, 877
Hpy8I GTNNAC 4 cut(s) 406, 750, 841, 1041
HpyAV CCTTC 1 cut(s) 914
HpyCH4III ACNGT 5 cut(s) 544, 610, 625, 800, 838
HpyCH4IV ACGT 1 cut(s) 825
HpyCH4V TGCA 4 cut(s) 257, 687, 772, 1132
HpyF10VI GCNNNNNNNGC 1 cut(s) 968
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 1 cut(s) 825
Hsp92II CATG 8 cut(s) 131, 173, 536, 619, 670, 679, 811, 1036
KpnI GGTACC 1 cut(s) 1000
Ksp22I TGATCA 2 cut(s) 166, 526
KspAI GTTAAC 1 cut(s) 1041
Kzo9I GATC 6 cut(s) 166, 229, 247, 358, 526, 1047
LguI GCTCTTC 1 cut(s) 590
LmnI GCTCC 1 cut(s) 562
LpnPI CCDG 7 cut(s) 12, 29, 652, 909, 942, 1013, 1029
Lsp1109I GCAGC 1 cut(s) 1090
LweI GCATC 1 cut(s) 781
MaeI CTAG 3 cut(s) 333, 602, 953
MaeII ACGT 1 cut(s) 825
MaeIII GTNAC 1 cut(s) 1018
MalI GATC 6 cut(s) 168, 231, 249, 360, 528, 1049
MboI GATC 6 cut(s) 166, 229, 247, 358, 526, 1047
MboII GAAGA 4 cut(s) 577, 706, 747, 796
MflI RGATCY 1 cut(s) 1047
MhlI GDGCHC 3 cut(s) 34, 567, 699
MmeI TCCRAC 2 cut(s) 36, 399
MroXI GAANNNNTTC 2 cut(s) 340, 625
MseI TTAA 6 cut(s) 152, 218, 252, 453, 1040, 1086
MslI CAYNNNNRTG 6 cut(s) 81, 132, 174, 354, 513, 991
MspI CCGG 2 cut(s) 16, 929
MwoI GCNNNNNNNGC 1 cut(s) 968
NdeII GATC 6 cut(s) 166, 229, 247, 358, 526, 1047
NheI GCTAGC 1 cut(s) 601
NlaIII CATG 8 cut(s) 131, 173, 536, 619, 670, 679, 811, 1036
NlaIV GGNNCC 2 cut(s) 835, 998
NmuCI GTSAC 1 cut(s) 1018
PagI TCATGA 1 cut(s) 666
PciSI GCTCTTC 1 cut(s) 590
PdmI GAANNNNTTC 2 cut(s) 340, 625
PfeI GAWTC 6 cut(s) 194, 547, 854, 890, 932, 1146
PinAI ACCGGT 1 cut(s) 15
PkrI GCNGC 1 cut(s) 1080
Ppu21I YACGTR 1 cut(s) 826
Psp124BI GAGCTC 1 cut(s) 699
PspN4I GGNNCC 2 cut(s) 835, 998
PspPI GGNCC 1 cut(s) 287
PstNI CAGNNNCTG 1 cut(s) 629
PsuI RGATCY 1 cut(s) 1047
RsaI GTAC 6 cut(s) 5, 42, 407, 998, 1031, 1060
RsaNI GTAC 6 cut(s) 4, 41, 406, 997, 1030, 1059
RseI CAYNNNNRTG 6 cut(s) 81, 132, 174, 354, 513, 991
SacI GAGCTC 1 cut(s) 699
SapI GCTCTTC 1 cut(s) 590
SaqAI TTAA 6 cut(s) 152, 218, 252, 453, 1040, 1086
SatI GCNGC 1 cut(s) 1079
Sau3AI GATC 6 cut(s) 166, 229, 247, 358, 526, 1047
Sau96I GGNCC 1 cut(s) 287
SduI GDGCHC 3 cut(s) 34, 567, 699
SetI ASST 9 cut(s) 9, 208, 467, 525, 699, 755, 828, 1002, 1095
SfaNI GCATC 1 cut(s) 781
SinI GGWCC 1 cut(s) 287
SmiI ATTTAAAT 1 cut(s) 153
SmiMI CAYNNNNRTG 6 cut(s) 81, 132, 174, 354, 513, 991
SmlI CTYRAG 4 cut(s) 69, 120, 734, 875
SmoI CTYRAG 4 cut(s) 69, 120, 734, 875
SnaBI TACGTA 1 cut(s) 826
SsiI CCGC 2 cut(s) 560, 950
SspI AATATT 1 cut(s) 190
SspMI CTAG 3 cut(s) 333, 602, 953
SstI GAGCTC 1 cut(s) 699
StyI CCWWGG 1 cut(s) 966
SwaI ATTTAAAT 1 cut(s) 153
TaaI ACNGT 5 cut(s) 544, 610, 625, 800, 838
TaiI ACGT 1 cut(s) 828
TaqI TCGA 3 cut(s) 295, 361, 1144
TatI WGTACW 1 cut(s) 405
TfiI GAWTC 6 cut(s) 194, 547, 854, 890, 932, 1146
Tru1I TTAA 6 cut(s) 152, 218, 252, 453, 1040, 1086
Tru9I TTAA 6 cut(s) 152, 218, 252, 453, 1040, 1086
TscAI CASTG 1 cut(s) 689
TseFI GTSAC 1 cut(s) 1018
TseI GCWGC 1 cut(s) 1078
Tsp45I GTSAC 1 cut(s) 1018
TspDTI ATGAA 8 cut(s) 144, 549, 604, 655, 683, 796, 879, 896
TspGWI ACGGA 1 cut(s) 75
TspRI CASTG 1 cut(s) 689
VpaK11BI GGWCC 1 cut(s) 287
XapI RAATTY 3 cut(s) 237, 338, 381
XbaI TCTAGA 1 cut(s) 332
XcmI CCANNNNNNNNNTGG 1 cut(s) 134
XmiI GTMKAC 1 cut(s) 749
XmnI GAANNNNTTC 2 cut(s) 340, 625
XspI CTAG 3 cut(s) 333, 602, 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.