MD02G1228500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012233
Physical Location & Seq
Forward (+)
27031829 .. 27034662
2834 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1228500.v1.1.491

Sequence Viewer

Length: 474 bp
ATGGCAGTCAGAAGCCTGAGCTCACTACCAGCCGTCCGATTCGAAGCCTCAAAATCAAACGGTGCTCTTCCTCCGCGGCGGAATCCAGAGGCGCGGCCCCGACTCTCCCTCTCCAAGCCCTCTTGGGTCGTCCGTACAGAGTCAAATGTTCACAGAGAAGTAAGGAAGAAGCCGGATCCTCCTTGTGAAGTCTGCACTGGGAGCGGAAGGGTCAATTGCCACGATTGTTGTGGAAGAGGAAGGACAAATTTTATTGACATGAAGATGCTCCCCAAAGGAGAATGGCCAAAATGGTGCAGGACTTGCGGTGGAAGTGGTCTCGGTTACTGCTCCCGCTGCCTTGGGACTGGTGAATATCGGTATATAATGGGATTTCAGTTCATGAAGATGAATTCATCTCATAGCAGAGACGATAAGAATGAGGCTCAATGCAAGCAAGACCGCCGTTCTGCAGCAGATTTCTACAAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

17.7

Weight (kDa)

9.66

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012233)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17668 AT3G17668
fragaria_vesca FvH4_7g08430 FvH4_7g08430
malus_domestica MD02G1228500.v1.1
prunus_persica Prupe.2G111200_v2.0.a1 Prupe.2G111200_v2.0.a1 Prupe.2G111200_v2.0.a1
pyrus_communis pycom02g19720
rosa_chinensis RchiOBHm_Chr0c20g0500251
rosa_laevigata RLG00000028995
rosa_multiflora Rmu_sc0010800.1_g000008
rosa_roxburghii Rroxscaffold_4G00311020
rosa_rugosa Rorug01G0165400
rosa_samantha Rh1AG180500 Rh1BG148700 Rh1CG167400 Rh1DG180600
rosa_wichuraiana Rw1G015000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 204
AccII CGCG 2 cut(s) 76, 94
AciI CCGC 8 cut(s) 74, 76, 79, 94, 204, 306, 334, 442
AclWI GGATC 2 cut(s) 170, 183
AcoI YGGCCR 1 cut(s) 284
AcsI RAATTY 2 cut(s) 247, 391
AfaI GTAC 1 cut(s) 136
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw21I GWGCWC 2 cut(s) 23, 67
Alw26I GTCTC 2 cut(s) 323, 402
AlwI GGATC 2 cut(s) 170, 183
AoxI GGCC 2 cut(s) 95, 284
ApeKI GCWGC 2 cut(s) 336, 452
ApoI RAATTY 2 cut(s) 247, 391
AspLEI GCGC 1 cut(s) 94
AspS9I GGNCC 1 cut(s) 96
AsuHPI GGTGA 1 cut(s) 362
AsuII TTCGAA 1 cut(s) 42
BalI TGGCCA 1 cut(s) 286
BamHI GGATCC 1 cut(s) 175
BanII GRGCYC 1 cut(s) 23
Bbv12I GWGCWC 2 cut(s) 23, 67
BbvI GCAGC 2 cut(s) 323, 464
BceAI ACGGC 2 cut(s) 17, 429
BcoDI GTCTC 2 cut(s) 323, 402
BfmI CTRYAG 1 cut(s) 450
BisI GCNGC 4 cut(s) 77, 95, 337, 453
BlsI GCNGC 4 cut(s) 78, 96, 338, 454
BmgT120I GGNCC 1 cut(s) 96
BmiI GGNNCC 2 cut(s) 98, 177
BmrI ACTGGG 1 cut(s) 207
BmsI GCATC 1 cut(s) 255
BmuI ACTGGG 1 cut(s) 207
Bpu10I CCTNAGC 1 cut(s) 17
Bpu14I TTCGAA 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 2 cut(s) 74, 340
Bse1I ACTGG 2 cut(s) 202, 352
BseDI CCNNGG 2 cut(s) 74, 340
BseMII CTCAG 1 cut(s) 8
BseNI ACTGG 2 cut(s) 202, 352
BseXI GCAGC 2 cut(s) 323, 464
BsgI GTGCAG 2 cut(s) 178, 316
Bsh1236I CGCG 2 cut(s) 76, 94
BshFI GGCC 2 cut(s) 97, 286
BsiHKAI GWGCWC 2 cut(s) 23, 67
BsiSI CCGG 1 cut(s) 173
BslFI GGGAC 1 cut(s) 358
BsmAI GTCTC 2 cut(s) 323, 402
BsmBI CGTCTC 1 cut(s) 402
BsmFI GGGAC 1 cut(s) 358
BsnI GGCC 2 cut(s) 97, 286
Bso31I GGTCTC 1 cut(s) 323
Bsp119I TTCGAA 1 cut(s) 42
Bsp1286I GDGCHC 2 cut(s) 23, 67
Bsp143I GATC 1 cut(s) 175
BspACI CCGC 8 cut(s) 74, 76, 79, 94, 204, 306, 334, 442
BspANI GGCC 2 cut(s) 97, 286
BspCNI CTCAG 1 cut(s) 9
BspFNI CGCG 2 cut(s) 76, 94
BspHI TCATGA 1 cut(s) 381
BspLI GGNNCC 2 cut(s) 98, 177
BspMAI CTGCAG 1 cut(s) 454
BspPI GGATC 2 cut(s) 170, 183
BspQI GCTCTTC 1 cut(s) 72
BspT104I TTCGAA 1 cut(s) 42
BspTNI GGTCTC 1 cut(s) 323
BsrBI CCGCTC 1 cut(s) 204
BsrI ACTGG 2 cut(s) 202, 352
BssECI CCNNGG 2 cut(s) 74, 340
BssMI GATC 1 cut(s) 175
BssT1I CCWWGG 1 cut(s) 340
Bst4CI ACNGT 1 cut(s) 62
Bst6I CTCTTC 2 cut(s) 72, 229
BstAPI GCANNNNNTGC 1 cut(s) 303
BstBI TTCGAA 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 434
BstDEI CTNAG 1 cut(s) 17
BstDSI CCRYGG 1 cut(s) 74
BstFNI CGCG 2 cut(s) 76, 94
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 1 cut(s) 178
BstMAI GTCTC 2 cut(s) 323, 402
BstMBI GATC 1 cut(s) 175
BstMWI GCNNNNNNNGC 3 cut(s) 201, 303, 336
BstSFI CTRYAG 1 cut(s) 450
BstUI CGCG 2 cut(s) 76, 94
BstV1I GCAGC 2 cut(s) 323, 464
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BsuRI GGCC 2 cut(s) 97, 286
BtgI CCRYGG 1 cut(s) 74
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 1 cut(s) 434
CciI TCATGA 1 cut(s) 381
CfoI GCGC 1 cut(s) 94
Cfr13I GGNCC 1 cut(s) 96
Cfr42I CCGCGG 1 cut(s) 77
Csp6I GTAC 1 cut(s) 135
CspCI CAANNNNNGTGG 2 cut(s) 209, 244
CviAII CATG 2 cut(s) 259, 382
CviJI RGCY 9 cut(s) 15, 21, 32, 47, 97, 118, 172, 286, 425
CviKI_1 RGCY 9 cut(s) 15, 21, 32, 47, 97, 118, 172, 286, 425
CviQI GTAC 1 cut(s) 135
DdeI CTNAG 1 cut(s) 17
DpnI GATC 1 cut(s) 177
DpnII GATC 1 cut(s) 175
EaeI YGGCCR 1 cut(s) 284
Eam1104I CTCTTC 2 cut(s) 72, 229
EarI CTCTTC 2 cut(s) 72, 229
EciI GGCGGA 1 cut(s) 94
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 340
Eco24I GRGCYC 1 cut(s) 23
Eco31I GGTCTC 1 cut(s) 323
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoRI GAATTC 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 23
ErhI CCWWGG 1 cut(s) 340
Esp3I CGTCTC 1 cut(s) 402
FaeI CATG 2 cut(s) 262, 385
FaiI YATR 5 cut(s) 260, 363, 365, 383, 402
FaqI GGGAC 1 cut(s) 358
FatI CATG 2 cut(s) 258, 381
FauI CCCGC 1 cut(s) 341
Fnu4HI GCNGC 4 cut(s) 77, 95, 337, 453
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 4 cut(s) 77, 95, 337, 453
GlaI GCGC 1 cut(s) 93
GluI GCNGC 4 cut(s) 77, 95, 337, 453
HaeIII GGCC 2 cut(s) 97, 286
HapII CCGG 1 cut(s) 173
HhaI GCGC 1 cut(s) 94
Hin1II CATG 2 cut(s) 262, 385
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HinfI GANTC 4 cut(s) 39, 82, 102, 140
HpaII CCGG 1 cut(s) 173
HphI GGTGA 1 cut(s) 362
Hpy166II GTNNAC 1 cut(s) 151
Hpy188I TCNGA 2 cut(s) 11, 38
Hpy188III TCNNGA 2 cut(s) 86, 382
Hpy8I GTNNAC 1 cut(s) 151
HpyAV CCTTC 2 cut(s) 201, 234
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4V TGCA 4 cut(s) 195, 297, 432, 452
HpyF10VI GCNNNNNNNGC 3 cut(s) 201, 303, 336
HpyF3I CTNAG 1 cut(s) 17
Hsp92II CATG 2 cut(s) 262, 385
HspAI GCGC 1 cut(s) 92
KspI CCGCGG 1 cut(s) 77
Kzo9I GATC 1 cut(s) 175
LguI GCTCTTC 1 cut(s) 72
LmnI GCTCC 3 cut(s) 201, 273, 335
LpnPI CCDG 7 cut(s) 29, 42, 99, 183, 186, 283, 333
Lsp1109I GCAGC 2 cut(s) 323, 464
LweI GCATC 1 cut(s) 255
MaeIII GTNAC 1 cut(s) 323
MalI GATC 1 cut(s) 177
MbiI CCGCTC 1 cut(s) 204
MboI GATC 1 cut(s) 175
MboII GAAGA 5 cut(s) 59, 178, 246, 274, 397
MfeI CAATTG 1 cut(s) 214
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 2 cut(s) 23, 67
MlsI TGGCCA 1 cut(s) 286
MluCI AATT 3 cut(s) 214, 247, 391
MluNI TGGCCA 1 cut(s) 286
MlyI GAGTC 2 cut(s) 96, 149
MnlI CCTC 8 cut(s) 58, 81, 82, 119, 130, 189, 230, 415
Mox20I TGGCCA 1 cut(s) 286
MscI TGGCCA 1 cut(s) 286
MslI CAYNNNNRTG 2 cut(s) 263, 386
Msp20I TGGCCA 1 cut(s) 286
MspA1I CMGCKG 2 cut(s) 76, 336
MspI CCGG 1 cut(s) 173
MunI CAATTG 1 cut(s) 214
MvnI CGCG 2 cut(s) 76, 94
MwoI GCNNNNNNNGC 3 cut(s) 201, 303, 336
NdeII GATC 1 cut(s) 175
NlaIII CATG 2 cut(s) 262, 385
NlaIV GGNNCC 2 cut(s) 98, 177
NspV TTCGAA 1 cut(s) 42
PagI TCATGA 1 cut(s) 381
PciSI GCTCTTC 1 cut(s) 72
PfeI GAWTC 2 cut(s) 39, 82
PkrI GCNGC 4 cut(s) 78, 96, 338, 454
PleI GAGTC 2 cut(s) 96, 148
PpsI GAGTC 2 cut(s) 96, 148
Psp124BI GAGCTC 1 cut(s) 23
PspN4I GGNNCC 2 cut(s) 98, 177
PspPI GGNCC 1 cut(s) 96
PstI CTGCAG 1 cut(s) 454
PsuI RGATCY 1 cut(s) 175
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
RseI CAYNNNNRTG 2 cut(s) 263, 386
SacI GAGCTC 1 cut(s) 23
SacII CCGCGG 1 cut(s) 77
SapI GCTCTTC 1 cut(s) 72
SatI GCNGC 4 cut(s) 77, 95, 337, 453
Sau3AI GATC 1 cut(s) 175
Sau96I GGNCC 1 cut(s) 96
SchI GAGTC 2 cut(s) 96, 149
SduI GDGCHC 2 cut(s) 23, 67
SetI ASST 1 cut(s) 23
SfaNI GCATC 1 cut(s) 255
SfcI CTRYAG 1 cut(s) 450
Sfr303I CCGCGG 1 cut(s) 77
SfuI TTCGAA 1 cut(s) 42
SgrBI CCGCGG 1 cut(s) 77
SmiMI CAYNNNNRTG 2 cut(s) 263, 386
Sse9I AATT 3 cut(s) 214, 247, 391
SsiI CCGC 8 cut(s) 74, 76, 79, 94, 204, 306, 334, 442
SstI GAGCTC 1 cut(s) 23
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 1 cut(s) 62
TaqI TCGA 1 cut(s) 42
TasI AATT 3 cut(s) 214, 247, 391
TauI GCSGC 2 cut(s) 79, 97
TfiI GAWTC 2 cut(s) 39, 82
TscAI CASTG 1 cut(s) 202
TseI GCWGC 2 cut(s) 336, 452
TspDTI ATGAA 5 cut(s) 275, 370, 384, 398, 404
TspGWI ACGGA 1 cut(s) 122
TspRI CASTG 1 cut(s) 202
XapI RAATTY 2 cut(s) 247, 391
XcmI CCANNNNNNNNNTGG 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.