Rorug01G0165400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0012233
Physical Location & Seq
Forward (+)
22557840 .. 22564340
6501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0165400.1

Sequence Viewer

Length: 1296 bp
ATGTCGAAACTGGTATACCCAAAGCTCAGTCGCACCGAGATCGTCACAATCCTCGTCGAGGCTCAGATCATCGCTATTTCGGAGCAGGATCTTCTCAACCCGAACCCGGATCTCATCGCCGACCTCTACACCCGGATCCTCACCACCTTCGACTTCCTCCCCGAGGAAGATGAGCAAGTTGATTTTGATGCCTTGGAGCTGCTCGAGAATCCGGATTTTCACGACAAGTCGGCCCGGAGCATCAAGCTGTACAACACACTGAAGCAAGTGGTGGCGTTATTGGATTGCCCTAAGAAATTTATCTACGCAGATCTCATAAGACCCGACCCGAATCGGACCGAGGTTTTTCTGAGTGCCCTTCTCAATTACCATGTATATAGAGAGACAAGAATAGATCTCGTAGCTAAAGTTGTGGATCAATTAACCGACCTTGAGAAGCAGCACATATGGTGGGGCGGTCAGGTTTCTCAGTGGAATACTGAGATTGCACACTACAAAGAAACAAGAGAAAAGGAGTTACCTCTTGTTCAGGAGGTAGATTCCAAAGTCAAAGAATTGCGCCAAACCATATCAGACCTTAACCTTGATCAAGTGAAACTGAGAACTTCTATTAGGAAGTTAAAGGAGAAGGCTGCTGAAATGGATAAAGAGATGTCTGATGCTGATTTTGATCTGCTAAAAAGTGATTCGGAAAGACAAGAGTTTCGTTCAAAAATTGTTCATTCACCAGATAAACTGCAGAGAGCTTTAGCAGAGAAGAAATTAATTAGAGAGAAGGCTACGAGTGATGAAATGTTAGCCATGCAATCTTTGAAGGAGAAAACTGCTGTTGATGAGGTGTATGCAAAGGTTTCCAAGAAATTGTTAAAACACTTGGCCCAGATGCATGCTATACAAGAACAGGTCAATTCTGCTAAATCTACTAGTAAAGACTACAGGGCTGTAAAAGAAAAAATAAGTGATGATGAAGTCCTAAGCAAGGTTCTTCATGATGAACTAATGAAACAGCAATCAAAAGTGAAAGAATTGAATGATTTCAAAAAGAAGTTAGAAAAAGAACGATATCTCAAGTTTGAGGAGGCTAGTAAAGAACTGAGTAATGTGAAGCTGGAGGTGGAATCTAGGAGGTGTGTGCTGGAAGCAAGGCAAAAGAATGTTGAAGCTGCAATAGCAGAGGTGGAAACTATAACTGCAAAGACTGCATCAGTAAGAAAATCTGGAGCAGCTGATCAGCAACAATTAGCTCGTCGAAGTGAGGAGATCACTAAAGAGATTCAGACAGTGGAATATAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

431

Amino Acids

49.88

Weight (kDa)

5.83

Isoelectric Point (pI)

35.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nuf2 PF03800 5 - 140 5.6e-27 Nuf2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012233)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17668 AT3G17668
fragaria_vesca FvH4_7g08430 FvH4_7g08430
malus_domestica MD02G1228500.v1.1
prunus_persica Prupe.2G111200_v2.0.a1 Prupe.2G111200_v2.0.a1 Prupe.2G111200_v2.0.a1
pyrus_communis pycom02g19720
rosa_chinensis RchiOBHm_Chr0c20g0500251
rosa_laevigata RLG00000028995
rosa_multiflora Rmu_sc0010800.1_g000008
rosa_roxburghii Rroxscaffold_4G00311020
rosa_rugosa Rorug01G0165400
rosa_samantha Rh1AG180500 Rh1BG148700 Rh1CG167400 Rh1DG180600
rosa_wichuraiana Rw1G015000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 15
AccIII TCCGGA 1 cut(s) 211
AciI CCGC 1 cut(s) 456
AclWI GGATC 5 cut(s) 96, 117, 130, 143, 423
AcsI RAATTY 1 cut(s) 296
AcuI CTGAAG 1 cut(s) 281
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 4 cut(s) 58, 106, 163, 979
AgsI TTSAA 5 cut(s) 711, 814, 1030, 1039, 1160
AhlI ACTAGT 1 cut(s) 923
Alw26I GTCTC 1 cut(s) 377
AlwI GGATC 5 cut(s) 96, 117, 130, 143, 423
Ama87I CYCGRG 2 cut(s) 161, 203
Aor13HI TCCGGA 1 cut(s) 211
AoxI GGCC 2 cut(s) 231, 876
ApeKI GCWGC 5 cut(s) 199, 439, 632, 1163, 1223
ApoI RAATTY 1 cut(s) 296
ArsI GACNNNNNNTTYG 2 cut(s) 687, 719
AseI ATTAAT 1 cut(s) 764
Asp700I GAANNNNTTC 1 cut(s) 1034
AspLEI GCGC 1 cut(s) 561
AspS9I GGNCC 3 cut(s) 232, 336, 877
AsuC2I CCSGG 3 cut(s) 107, 133, 235
AsuHPI GGTGA 2 cut(s) 133, 717
AvaI CYCGRG 2 cut(s) 161, 203
AvaII GGWCC 1 cut(s) 336
BaeGI GKGCMC 1 cut(s) 358
BamHI GGATCC 1 cut(s) 135
BbvI GCAGC 5 cut(s) 186, 451, 619, 1150, 1235
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BclI TGATCA 2 cut(s) 586, 1228
BcnI CCSGG 3 cut(s) 107, 133, 235
BcoDI GTCTC 1 cut(s) 377
BcuI ACTAGT 1 cut(s) 923
BfaI CTAG 4 cut(s) 924, 1083, 1122, 1294
BfmI CTRYAG 2 cut(s) 737, 934
BglII AGATCT 2 cut(s) 310, 394
BisI GCNGC 5 cut(s) 200, 440, 633, 1164, 1224
BlsI GCNGC 5 cut(s) 201, 441, 634, 1165, 1225
Bme1390I CCNGG 3 cut(s) 107, 133, 235
Bme18I GGWCC 1 cut(s) 336
BmeT110I CYCGRG 2 cut(s) 161, 203
BmgT120I GGNCC 3 cut(s) 232, 336, 877
BmiI GGNNCC 1 cut(s) 137
BmrFI CCNGG 3 cut(s) 107, 133, 235
BmsI GCATC 5 cut(s) 178, 249, 649, 873, 1211
BpmI CTGGAG 2 cut(s) 1130, 1239
Bpu10I CCTNAGC 1 cut(s) 974
BpuEI CTTGAG 2 cut(s) 452, 1052
BpuMI CCSGG 3 cut(s) 107, 133, 235
BsaBI GATNNNNATC 1 cut(s) 669
BsaJI CCNNGG 3 cut(s) 162, 192, 339
BsaWI WCCGGW 1 cut(s) 211
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 106, 163, 979
Bse1I ACTGG 1 cut(s) 15
Bse8I GATNNNNATC 1 cut(s) 669
BseAI TCCGGA 1 cut(s) 211
BseDI CCNNGG 3 cut(s) 162, 192, 339
BseJI GATNNNNATC 1 cut(s) 669
BseLI CCNNNNNNNGG 4 cut(s) 58, 106, 163, 979
BseMII CTCAG 7 cut(s) 40, 77, 341, 471, 482, 590, 1085
BseNI ACTGG 1 cut(s) 15
BseRI GAGGAG 2 cut(s) 1091, 1271
BseSI GKGCMC 1 cut(s) 358
BseXI GCAGC 5 cut(s) 186, 451, 619, 1150, 1235
BshFI GGCC 2 cut(s) 233, 878
BsiHKCI CYCGRG 2 cut(s) 161, 203
BsiSI CCGG 4 cut(s) 107, 133, 212, 235
BslI CCNNNNNNNGG 4 cut(s) 58, 106, 163, 979
BsmAI GTCTC 1 cut(s) 377
BsnI GGCC 2 cut(s) 233, 878
BsoBI CYCGRG 2 cut(s) 161, 203
Bsp1286I GDGCHC 1 cut(s) 358
Bsp13I TCCGGA 1 cut(s) 211
Bsp1407I TGTACA 1 cut(s) 249
BspACI CCGC 1 cut(s) 456
BspANI GGCC 2 cut(s) 233, 878
BspCNI CTCAG 7 cut(s) 39, 76, 342, 472, 481, 591, 1086
BspEI TCCGGA 1 cut(s) 211
BspHI TCATGA 1 cut(s) 988
BspLI GGNNCC 1 cut(s) 137
BspMAI CTGCAG 1 cut(s) 741
BspPI GGATC 5 cut(s) 96, 117, 130, 143, 423
BsrGI TGTACA 1 cut(s) 249
BsrI ACTGG 1 cut(s) 15
BssECI CCNNGG 3 cut(s) 162, 192, 339
BssNAI GTATAC 1 cut(s) 16
BssT1I CCWWGG 1 cut(s) 192
Bst1107I GTATAC 1 cut(s) 16
Bst4CI ACNGT 1 cut(s) 1282
BstAPI GCANNNNNTGC 1 cut(s) 1199
BstAUI TGTACA 1 cut(s) 249
BstC8I GCNNGC 1 cut(s) 888
BstDEI CTNAG 9 cut(s) 26, 63, 291, 350, 468, 480, 599, 974, 1094
BstENI CCTNNNNNAGG 3 cut(s) 56, 161, 977
BstHHI GCGC 1 cut(s) 561
BstMAI GTCTC 1 cut(s) 377
BstMWI GCNNNNNNNGC 2 cut(s) 1169, 1199
BstNSI RCATGY 1 cut(s) 890
BstSCI CCNGG 3 cut(s) 105, 131, 233
BstSFI CTRYAG 2 cut(s) 737, 934
BstSLI GKGCMC 1 cut(s) 358
BstV1I GCAGC 5 cut(s) 186, 451, 619, 1150, 1235
BstX2I RGATCY 5 cut(s) 88, 109, 135, 310, 394
BstYI RGATCY 5 cut(s) 88, 109, 135, 310, 394
BstZ17I GTATAC 1 cut(s) 16
BsuRI GGCC 2 cut(s) 233, 878
BtgZI GCGATG 2 cut(s) 55, 100
BtsIMutI CAGTG 3 cut(s) 257, 476, 1287
Cac8I GCNNGC 1 cut(s) 888
CciI TCATGA 1 cut(s) 988
CfoI GCGC 1 cut(s) 561
Cfr13I GGNCC 3 cut(s) 232, 336, 877
CpoI CGGWCCG 1 cut(s) 336
Csp6I GTAC 1 cut(s) 250
CspI CGGWCCG 1 cut(s) 336
CviAII CATG 4 cut(s) 371, 802, 887, 989
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 9 cut(s) 26, 63, 291, 350, 468, 480, 599, 974, 1094
Eco130I CCWWGG 1 cut(s) 192
Eco32I GATATC 1 cut(s) 1064
Eco47I GGWCC 1 cut(s) 336
Eco57I CTGAAG 1 cut(s) 281
Eco88I CYCGRG 2 cut(s) 161, 203
EcoNI CCTNNNNNAGG 3 cut(s) 56, 161, 977
EcoRV GATATC 1 cut(s) 1064
EcoT14I CCWWGG 1 cut(s) 192
EcoT22I ATGCAT 1 cut(s) 888
ErhI CCWWGG 1 cut(s) 192
FaeI CATG 4 cut(s) 374, 805, 890, 992
FatI CATG 4 cut(s) 370, 801, 886, 988
FauNDI CATATG 1 cut(s) 446
FbaI TGATCA 2 cut(s) 586, 1228
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 5 cut(s) 200, 440, 633, 1164, 1224
Fsp4HI GCNGC 5 cut(s) 200, 440, 633, 1164, 1224
FspBI CTAG 4 cut(s) 924, 1083, 1122, 1294
GlaI GCGC 1 cut(s) 560
GluI GCNGC 5 cut(s) 200, 440, 633, 1164, 1224
GsuI CTGGAG 2 cut(s) 1130, 1239
HaeIII GGCC 2 cut(s) 233, 878
HapII CCGG 4 cut(s) 107, 133, 212, 235
HhaI GCGC 1 cut(s) 561
Hin1II CATG 4 cut(s) 374, 805, 890, 992
Hin6I GCGC 1 cut(s) 559
HinP1I GCGC 1 cut(s) 559
HinfI GANTC 6 cut(s) 208, 331, 539, 686, 1118, 1273
HpaII CCGG 4 cut(s) 107, 133, 212, 235
HphI GGTGA 2 cut(s) 133, 717
Hpy166II GTNNAC 1 cut(s) 16
Hpy188I TCNGA 8 cut(s) 66, 82, 336, 351, 574, 658, 691, 1278
Hpy188III TCNNGA 6 cut(s) 205, 212, 221, 530, 989, 1218
Hpy8I GTNNAC 1 cut(s) 16
Hpy99I CGWCG 2 cut(s) 59, 1251
HpyAV CCTTC 5 cut(s) 157, 368, 622, 769, 808
HpyCH4III ACNGT 1 cut(s) 1282
HpyCH4V TGCA 8 cut(s) 488, 739, 805, 845, 886, 1166, 1193, 1202
HpyF10VI GCNNNNNNNGC 2 cut(s) 1169, 1199
HpyF3I CTNAG 9 cut(s) 26, 63, 291, 350, 468, 480, 599, 974, 1094
Hsp92II CATG 4 cut(s) 374, 805, 890, 992
HspAI GCGC 1 cut(s) 559
Kpn2I TCCGGA 1 cut(s) 211
Ksp22I TGATCA 2 cut(s) 586, 1228
LmnI GCTCC 4 cut(s) 82, 196, 237, 1220
Lsp1109I GCAGC 5 cut(s) 186, 451, 619, 1150, 1235
LweI GCATC 5 cut(s) 178, 249, 649, 873, 1211
MaeI CTAG 4 cut(s) 924, 1083, 1122, 1294
MaeIII GTNAC 2 cut(s) 43, 516
MboII GAAGA 4 cut(s) 83, 179, 769, 977
MflI RGATCY 5 cut(s) 88, 109, 135, 310, 394
MhlI GDGCHC 1 cut(s) 358
Mph1103I ATGCAT 1 cut(s) 888
MroI TCCGGA 1 cut(s) 211
MroXI GAANNNNTTC 1 cut(s) 1034
MseI TTAA 5 cut(s) 422, 579, 620, 764, 866
MspA1I CMGCKG 1 cut(s) 1226
MspI CCGG 4 cut(s) 107, 133, 212, 235
MspR9I CCNGG 3 cut(s) 107, 133, 235
MwoI GCNNNNNNNGC 2 cut(s) 1169, 1199
NciI CCSGG 3 cut(s) 107, 133, 235
NdeI CATATG 1 cut(s) 446
NlaIII CATG 4 cut(s) 374, 805, 890, 992
NlaIV GGNNCC 1 cut(s) 137
NmuCI GTSAC 1 cut(s) 43
NsiI ATGCAT 1 cut(s) 888
NspI RCATGY 1 cut(s) 890
PaeI GCATGC 1 cut(s) 890
PaeR7I CTCGAG 1 cut(s) 203
PagI TCATGA 1 cut(s) 988
PdmI GAANNNNTTC 1 cut(s) 1034
PfeI GAWTC 6 cut(s) 208, 331, 539, 686, 1118, 1273
PkrI GCNGC 5 cut(s) 201, 441, 634, 1165, 1225
PshBI ATTAAT 1 cut(s) 764
PspN4I GGNNCC 1 cut(s) 137
PspPI GGNCC 3 cut(s) 232, 336, 877
PstI CTGCAG 1 cut(s) 741
PsuI RGATCY 5 cut(s) 88, 109, 135, 310, 394
PvuII CAGCTG 1 cut(s) 1226
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
Rsr2I CGGWCCG 1 cut(s) 336
RsrII CGGWCCG 1 cut(s) 336
SaqAI TTAA 5 cut(s) 422, 579, 620, 764, 866
SatI GCNGC 5 cut(s) 200, 440, 633, 1164, 1224
Sau96I GGNCC 3 cut(s) 232, 336, 877
ScrFI CCNGG 3 cut(s) 107, 133, 235
SduI GDGCHC 1 cut(s) 358
SfaNI GCATC 5 cut(s) 178, 249, 649, 873, 1211
SfcI CTRYAG 2 cut(s) 737, 934
Sfr274I CTCGAG 1 cut(s) 203
SinI GGWCC 1 cut(s) 336
SlaI CTCGAG 1 cut(s) 203
SmlI CTYRAG 3 cut(s) 203, 431, 1067
SmoI CTYRAG 3 cut(s) 203, 431, 1067
SpeI ACTAGT 1 cut(s) 923
SphI GCATGC 1 cut(s) 890
SsiI CCGC 1 cut(s) 456
SspMI CTAG 4 cut(s) 924, 1083, 1122, 1294
StyD4I CCNGG 3 cut(s) 105, 131, 233
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 1 cut(s) 1282
TaqI TCGA 5 cut(s) 5, 57, 150, 204, 1249
TaqII GACCGA 1 cut(s) 353
TatI WGTACW 1 cut(s) 249
TfiI GAWTC 6 cut(s) 208, 331, 539, 686, 1118, 1273
Tru1I TTAA 5 cut(s) 422, 579, 620, 764, 866
Tru9I TTAA 5 cut(s) 422, 579, 620, 764, 866
TscAI CASTG 3 cut(s) 264, 476, 1287
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 5 cut(s) 199, 439, 632, 1163, 1223
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 6 cut(s) 710, 804, 977, 981, 1008, 1016
TspRI CASTG 3 cut(s) 264, 476, 1287
VpaK11BI GGWCC 1 cut(s) 336
VspI ATTAAT 1 cut(s) 764
XagI CCTNNNNNAGG 3 cut(s) 56, 161, 977
XapI RAATTY 1 cut(s) 296
XceI RCATGY 1 cut(s) 890
XhoI CTCGAG 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 15
XmnI GAANNNNTTC 1 cut(s) 1034
XspI CTAG 4 cut(s) 924, 1083, 1122, 1294
Zsp2I ATGCAT 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.