RLG00000028995

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0012233
Physical Location & Seq
Forward (+)
31213601 .. 31217093
3493 bp
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UTR
Exon/CDS
Intron
RLM00000028995

Sequence Viewer

Length: 471 bp
ATGGCACTCAGAATCGTCCTGAGCCCAGTAACCACCGTCCGATTGGAAGCCACAAATTCCAACGGTGCTCTTTCCCCACGGAATCTAGACCCGTCGCCCCGACTCTCCTTCTCCAAACCTTCTTGGGTCGTCCGCACTGAGTCAAATGTTCGTAAAGAAGTAAGAAAGGCCCCGGATCCTCCTTGTGATATCTGCACAGGGAGTGGAAGGATTGATTGCCACTTTTGTCAGGGAAAAGGGAGGACAAACTCTGTTGACATGCAAATGCTACCCAAAGGAAAATGGCCAAGATGGTGCAAGACTTGTGGTGGAAGTGGTCTCAGTTACTGCTCCCGCTGCCTTGGAACTGGAGAGTATAGGTATATAATGGGCTTTCATTTCATGAAGATGGATTCAGCTCATACAGGTGACAATCTGAATGCAATTCAAAGTAATCAAAAACGATGCACTGCTAAGGATTTATATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

157

Amino Acids

17.38

Weight (kDa)

9.37

Isoelectric Point (pI)

34.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012233)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17668 AT3G17668
fragaria_vesca FvH4_7g08430 FvH4_7g08430
malus_domestica MD02G1228500.v1.1
prunus_persica Prupe.2G111200_v2.0.a1 Prupe.2G111200_v2.0.a1 Prupe.2G111200_v2.0.a1
pyrus_communis pycom02g19720
rosa_chinensis RchiOBHm_Chr0c20g0500251
rosa_laevigata RLG00000028995
rosa_multiflora Rmu_sc0010800.1_g000008
rosa_roxburghii Rroxscaffold_4G00311020
rosa_rugosa Rorug01G0165400
rosa_samantha Rh1AG180500 Rh1BG148700 Rh1CG167400 Rh1DG180600
rosa_wichuraiana Rw1G015000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 133, 334
AclWI GGATC 2 cut(s) 170, 183
AcoI YGGCCR 1 cut(s) 284
AcsI RAATTY 1 cut(s) 55
AgsI TTSAA 1 cut(s) 428
AluBI AGCT 1 cut(s) 398
AluI AGCT 1 cut(s) 398
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 1 cut(s) 323
AlwI GGATC 2 cut(s) 170, 183
AlwNI CAGNNNCTG 1 cut(s) 327
AoxI GGCC 2 cut(s) 168, 284
ApeKI GCWGC 1 cut(s) 336
ApoI RAATTY 1 cut(s) 55
AspS9I GGNCC 1 cut(s) 169
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 1 cut(s) 419
BalI TGGCCA 1 cut(s) 286
BamHI GGATCC 1 cut(s) 175
BanII GRGCYC 1 cut(s) 26
Bbv12I GWGCWC 1 cut(s) 70
BbvI GCAGC 1 cut(s) 323
BccI CCATC 2 cut(s) 285, 382
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 1 cut(s) 323
BfaI CTAG 1 cut(s) 86
BisI GCNGC 1 cut(s) 337
BlsI GCNGC 1 cut(s) 338
Bme1390I CCNGG 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 169
BmiI GGNNCC 2 cut(s) 171, 177
BmrFI CCNGG 1 cut(s) 173
BmrI ACTGGG 1 cut(s) 20
BmsI GCATC 1 cut(s) 434
BmuI ACTGGG 1 cut(s) 20
BpmI CTGGAG 1 cut(s) 369
Bpu10I CCTNAGC 2 cut(s) 20, 453
BpuMI CCSGG 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 3 cut(s) 77, 171, 340
Bse1I ACTGG 2 cut(s) 26, 352
BseDI CCNNGG 3 cut(s) 77, 171, 340
BseMII CTCAG 4 cut(s) 11, 22, 129, 334
BseNI ACTGG 2 cut(s) 26, 352
BseXI GCAGC 1 cut(s) 323
BsgI GTGCAG 1 cut(s) 178
BshFI GGCC 2 cut(s) 170, 286
BsiHKAI GWGCWC 1 cut(s) 70
BsiSI CCGG 1 cut(s) 173
BsmAI GTCTC 1 cut(s) 323
BsmI GAATGC 1 cut(s) 424
BsnI GGCC 2 cut(s) 170, 286
Bso31I GGTCTC 1 cut(s) 323
Bsp1286I GDGCHC 2 cut(s) 26, 70
Bsp143I GATC 1 cut(s) 175
BspACI CCGC 2 cut(s) 133, 334
BspANI GGCC 2 cut(s) 170, 286
BspCNI CTCAG 4 cut(s) 12, 21, 130, 333
BspHI TCATGA 1 cut(s) 381
BspLI GGNNCC 2 cut(s) 171, 177
BspPI GGATC 2 cut(s) 170, 183
BspTNI GGTCTC 1 cut(s) 323
BsrI ACTGG 2 cut(s) 26, 352
BssECI CCNNGG 3 cut(s) 77, 171, 340
BssMI GATC 1 cut(s) 175
BssT1I CCWWGG 1 cut(s) 340
Bst4CI ACNGT 2 cut(s) 37, 65
BstDEI CTNAG 5 cut(s) 8, 20, 138, 320, 453
BstDSI CCRYGG 1 cut(s) 77
BstKTI GATC 1 cut(s) 178
BstMAI GTCTC 1 cut(s) 323
BstMBI GATC 1 cut(s) 175
BstMWI GCNNNNNNNGC 1 cut(s) 336
BstNSI RCATGY 1 cut(s) 262
BstSCI CCNGG 1 cut(s) 171
BstV1I GCAGC 1 cut(s) 323
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BsuRI GGCC 2 cut(s) 170, 286
BtgI CCRYGG 1 cut(s) 77
BtsI GCAGTG 1 cut(s) 447
BtsIMutI CAGTG 2 cut(s) 135, 447
CaiI CAGNNNCTG 1 cut(s) 327
CciI TCATGA 1 cut(s) 381
Cfr13I GGNCC 1 cut(s) 169
CspCI CAANNNNNGTGG 2 cut(s) 286, 321
CviAII CATG 2 cut(s) 259, 382
CviJI RGCY 6 cut(s) 24, 50, 170, 286, 372, 398
CviKI_1 RGCY 6 cut(s) 24, 50, 170, 286, 372, 398
DdeI CTNAG 5 cut(s) 8, 20, 138, 320, 453
DpnI GATC 1 cut(s) 177
DpnII GATC 1 cut(s) 175
EaeI YGGCCR 1 cut(s) 284
Eco130I CCWWGG 1 cut(s) 340
Eco24I GRGCYC 1 cut(s) 26
Eco31I GGTCTC 1 cut(s) 323
Eco32I GATATC 1 cut(s) 190
EcoO109I RGGNCCY 1 cut(s) 169
EcoRV GATATC 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 340
FaeI CATG 2 cut(s) 262, 385
FaiI YATR 8 cut(s) 260, 357, 363, 365, 383, 402, 463, 465
FatI CATG 2 cut(s) 258, 381
FauI CCCGC 1 cut(s) 341
Fnu4HI GCNGC 1 cut(s) 337
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 1 cut(s) 337
FspBI CTAG 1 cut(s) 86
GluI GCNGC 1 cut(s) 337
GsuI CTGGAG 1 cut(s) 369
HaeIII GGCC 2 cut(s) 170, 286
HapII CCGG 1 cut(s) 173
Hin1II CATG 2 cut(s) 262, 385
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HinfI GANTC 5 cut(s) 12, 82, 102, 140, 392
HpaII CCGG 1 cut(s) 173
HphI GGTGA 1 cut(s) 419
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 3 cut(s) 11, 41, 417
Hpy188III TCNNGA 3 cut(s) 19, 86, 382
Hpy8I GTNNAC 1 cut(s) 256
Hpy99I CGWCG 1 cut(s) 97
HpyAV CCTTC 3 cut(s) 118, 129, 201
HpyCH4III ACNGT 2 cut(s) 37, 65
HpyCH4V TGCA 5 cut(s) 195, 262, 297, 422, 447
HpyF10VI GCNNNNNNNGC 1 cut(s) 336
HpyF3I CTNAG 5 cut(s) 8, 20, 138, 320, 453
Hsp92II CATG 2 cut(s) 262, 385
Kzo9I GATC 1 cut(s) 175
LmnI GCTCC 1 cut(s) 335
LpnPI CCDG 7 cut(s) 32, 39, 183, 186, 215, 333, 390
Lsp1109I GCAGC 1 cut(s) 323
LweI GCATC 1 cut(s) 434
MaeI CTAG 1 cut(s) 86
MaeIII GTNAC 3 cut(s) 28, 323, 407
MalI GATC 1 cut(s) 177
MboI GATC 1 cut(s) 175
MboII GAAGA 1 cut(s) 397
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 2 cut(s) 26, 70
MlsI TGGCCA 1 cut(s) 286
MluCI AATT 2 cut(s) 55, 423
MluNI TGGCCA 1 cut(s) 286
MlyI GAGTC 2 cut(s) 96, 149
MmeI TCCRAC 1 cut(s) 84
MnlI CCTC 2 cut(s) 189, 234
Mox20I TGGCCA 1 cut(s) 286
MscI TGGCCA 1 cut(s) 286
MslI CAYNNNNRTG 3 cut(s) 263, 386, 405
Msp20I TGGCCA 1 cut(s) 286
MspA1I CMGCKG 1 cut(s) 336
MspI CCGG 1 cut(s) 173
MspR9I CCNGG 1 cut(s) 173
Mva1269I GAATGC 1 cut(s) 424
MwoI GCNNNNNNNGC 1 cut(s) 336
NciI CCSGG 1 cut(s) 173
NdeII GATC 1 cut(s) 175
NlaIII CATG 2 cut(s) 262, 385
NlaIV GGNNCC 2 cut(s) 171, 177
NmuCI GTSAC 1 cut(s) 407
NspI RCATGY 1 cut(s) 262
PagI TCATGA 1 cut(s) 381
PctI GAATGC 1 cut(s) 424
PfeI GAWTC 3 cut(s) 12, 82, 392
PkrI GCNGC 1 cut(s) 338
PleI GAGTC 2 cut(s) 96, 148
PpsI GAGTC 2 cut(s) 96, 148
PspN4I GGNNCC 2 cut(s) 171, 177
PspPI GGNCC 1 cut(s) 169
PstNI CAGNNNCTG 1 cut(s) 327
PsuI RGATCY 1 cut(s) 175
RseI CAYNNNNRTG 3 cut(s) 263, 386, 405
SatI GCNGC 1 cut(s) 337
Sau3AI GATC 1 cut(s) 175
Sau96I GGNCC 1 cut(s) 169
SchI GAGTC 2 cut(s) 96, 149
ScrFI CCNGG 1 cut(s) 173
SduI GDGCHC 2 cut(s) 26, 70
SetI ASST 4 cut(s) 121, 362, 400, 409
SfaNI GCATC 1 cut(s) 434
SmiMI CAYNNNNRTG 3 cut(s) 263, 386, 405
Sse9I AATT 2 cut(s) 55, 423
SsiI CCGC 2 cut(s) 133, 334
SspMI CTAG 1 cut(s) 86
StyD4I CCNGG 1 cut(s) 171
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 2 cut(s) 37, 65
TasI AATT 2 cut(s) 55, 423
TfiI GAWTC 3 cut(s) 12, 82, 392
TscAI CASTG 2 cut(s) 142, 454
TseFI GTSAC 1 cut(s) 407
TseI GCWGC 1 cut(s) 336
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 3 cut(s) 365, 370, 398
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 2 cut(s) 142, 454
XapI RAATTY 1 cut(s) 55
XbaI TCTAGA 1 cut(s) 85
XceI RCATGY 1 cut(s) 262
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
XspI CTAG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.