pycom02g19720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012233
Physical Location & Seq
Forward (+)
17635496 .. 17637466
1971 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g19720.3

Sequence Viewer

Length: 486 bp
ATGGCAGTCAGAAGCCTGAGCTCACTACCAGCCGTCCGATTCGAAGCCTCAAAATCAAACGGTGCTCCTCCTCCGCCTCTTCCTCCGCCGCGGAATCCGGAGCCGCGGCCCCGACTCTCCCTCTCCAAGCCCTCTTGGGTCGTCCGTGCAGAGTCAAATGTTCACAGAGAAGTAAGGAAGAAACCGGATCCTCCTTGTGAGGTCTGCACTGGGAGTGGAAGGGTCAATTGCCATGATTGTTGTGGAAGAGGAAGGACAAATTTTATTGACATGAAAATGCTCCCCAAAGGAGAATGGCCAAAATGGTGCAGGACTTGCGGTGGAAGCGGTCTCGGTTACTGCTCCCGCTGCCTTGGGACCGGTGAGTATCGGTATATAATGGGATTTCAGTTCATGAAGATGAGTTCGTCTCATAGCAGAGACATTAAGAATGAGGCTCAACGCAAGCAAGATCGCTGTTCTGCTGCAGATTTCTACGAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

17.99

Weight (kDa)

9.5

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012233)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17668 AT3G17668
fragaria_vesca FvH4_7g08430 FvH4_7g08430
malus_domestica MD02G1228500.v1.1
prunus_persica Prupe.2G111200_v2.0.a1 Prupe.2G111200_v2.0.a1 Prupe.2G111200_v2.0.a1
pyrus_communis pycom02g19720
rosa_chinensis RchiOBHm_Chr0c20g0500251
rosa_laevigata RLG00000028995
rosa_multiflora Rmu_sc0010800.1_g000008
rosa_roxburghii Rroxscaffold_4G00311020
rosa_rugosa Rorug01G0165400
rosa_samantha Rh1AG180500 Rh1BG148700 Rh1CG167400 Rh1DG180600
rosa_wichuraiana Rw1G015000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 91, 106
AccIII TCCGGA 1 cut(s) 97
AciI CCGC 9 cut(s) 74, 86, 89, 91, 104, 106, 318, 327, 346
AclWI GGATC 2 cut(s) 182, 195
AcoI YGGCCR 1 cut(s) 296
AcsI RAATTY 1 cut(s) 259
AgeI ACCGGT 1 cut(s) 359
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw21I GWGCWC 2 cut(s) 23, 67
Alw26I GTCTC 3 cut(s) 335, 414, 414
AlwI GGATC 2 cut(s) 182, 195
Aor13HI TCCGGA 1 cut(s) 97
AoxI GGCC 2 cut(s) 107, 296
ApeKI GCWGC 2 cut(s) 348, 464
ApoI RAATTY 1 cut(s) 259
AsiGI ACCGGT 1 cut(s) 359
AspS9I GGNCC 2 cut(s) 108, 357
AsuHPI GGTGA 1 cut(s) 374
AsuII TTCGAA 1 cut(s) 42
AvaII GGWCC 1 cut(s) 357
BalI TGGCCA 1 cut(s) 298
BamHI GGATCC 1 cut(s) 187
BanII GRGCYC 1 cut(s) 23
Bbv12I GWGCWC 2 cut(s) 23, 67
BbvI GCAGC 2 cut(s) 335, 451
BceAI ACGGC 1 cut(s) 17
BcoDI GTCTC 3 cut(s) 335, 414, 414
BfmI CTRYAG 1 cut(s) 465
BisI GCNGC 5 cut(s) 89, 104, 107, 349, 465
BlsI GCNGC 5 cut(s) 90, 105, 108, 350, 466
Bme18I GGWCC 1 cut(s) 357
BmgT120I GGNCC 2 cut(s) 108, 357
BmiI GGNNCC 4 cut(s) 102, 110, 189, 358
BmrI ACTGGG 1 cut(s) 219
BmuI ACTGGG 1 cut(s) 219
BplI GAGNNNNNCTC 2 cut(s) 394, 426
Bpu10I CCTNAGC 1 cut(s) 17
Bpu14I TTCGAA 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 335
BsaJI CCNNGG 3 cut(s) 89, 104, 352
BsaWI WCCGGW 3 cut(s) 97, 184, 359
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 1 cut(s) 214
BseAI TCCGGA 1 cut(s) 97
BseDI CCNNGG 3 cut(s) 89, 104, 352
BseMII CTCAG 1 cut(s) 8
BseNI ACTGG 1 cut(s) 214
BseRI GAGGAG 2 cut(s) 57, 60
BseXI GCAGC 2 cut(s) 335, 451
BsgI GTGCAG 3 cut(s) 168, 190, 328
Bsh1236I CGCG 2 cut(s) 91, 106
BshFI GGCC 2 cut(s) 109, 298
BshTI ACCGGT 1 cut(s) 359
BsiHKAI GWGCWC 2 cut(s) 23, 67
BsiSI CCGG 3 cut(s) 98, 185, 360
BslFI GGGAC 1 cut(s) 370
BsmAI GTCTC 3 cut(s) 335, 414, 414
BsmBI CGTCTC 1 cut(s) 414
BsmFI GGGAC 1 cut(s) 370
BsnI GGCC 2 cut(s) 109, 298
Bso31I GGTCTC 1 cut(s) 335
Bsp119I TTCGAA 1 cut(s) 42
Bsp1286I GDGCHC 2 cut(s) 23, 67
Bsp13I TCCGGA 1 cut(s) 97
Bsp143I GATC 2 cut(s) 187, 451
BspACI CCGC 9 cut(s) 74, 86, 89, 91, 104, 106, 318, 327, 346
BspANI GGCC 2 cut(s) 109, 298
BspCNI CTCAG 1 cut(s) 9
BspEI TCCGGA 1 cut(s) 97
BspFNI CGCG 2 cut(s) 91, 106
BspHI TCATGA 1 cut(s) 393
BspLI GGNNCC 4 cut(s) 102, 110, 189, 358
BspMAI CTGCAG 1 cut(s) 469
BspPI GGATC 2 cut(s) 182, 195
BspT104I TTCGAA 1 cut(s) 42
BspTNI GGTCTC 1 cut(s) 335
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 1 cut(s) 214
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 3 cut(s) 89, 104, 352
BssMI GATC 2 cut(s) 187, 451
BssT1I CCWWGG 1 cut(s) 352
Bst4CI ACNGT 1 cut(s) 62
Bst6I CTCTTC 2 cut(s) 84, 241
BstAPI GCANNNNNTGC 1 cut(s) 315
BstBI TTCGAA 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 446
BstDEI CTNAG 1 cut(s) 17
BstDSI CCRYGG 2 cut(s) 89, 104
BstFNI CGCG 2 cut(s) 91, 106
BstKTI GATC 2 cut(s) 190, 454
BstMAI GTCTC 3 cut(s) 335, 414, 414
BstMBI GATC 2 cut(s) 187, 451
BstMWI GCNNNNNNNGC 3 cut(s) 315, 324, 348
BstSFI CTRYAG 1 cut(s) 465
BstUI CGCG 2 cut(s) 91, 106
BstV1I GCAGC 2 cut(s) 335, 451
BstX2I RGATCY 1 cut(s) 187
BstYI RGATCY 1 cut(s) 187
BsuRI GGCC 2 cut(s) 109, 298
BtgI CCRYGG 2 cut(s) 89, 104
BtsIMutI CAGTG 1 cut(s) 207
Cac8I GCNNGC 1 cut(s) 446
CciI TCATGA 1 cut(s) 393
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 2 cut(s) 108, 357
Cfr42I CCGCGG 2 cut(s) 92, 107
CspAI ACCGGT 1 cut(s) 359
CviAII CATG 3 cut(s) 233, 271, 394
CviJI RGCY 9 cut(s) 15, 21, 32, 47, 103, 109, 130, 298, 437
CviKI_1 RGCY 9 cut(s) 15, 21, 32, 47, 103, 109, 130, 298, 437
DdeI CTNAG 1 cut(s) 17
DpnI GATC 2 cut(s) 189, 453
DpnII GATC 2 cut(s) 187, 451
EaeI YGGCCR 1 cut(s) 296
Eam1104I CTCTTC 2 cut(s) 84, 241
EarI CTCTTC 2 cut(s) 84, 241
EciI GGCGGA 2 cut(s) 63, 75
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 352
Eco24I GRGCYC 1 cut(s) 23
Eco31I GGTCTC 1 cut(s) 335
Eco47I GGWCC 1 cut(s) 357
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 352
EcoT38I GRGCYC 1 cut(s) 23
ErhI CCWWGG 1 cut(s) 352
Esp3I CGTCTC 1 cut(s) 414
FaeI CATG 3 cut(s) 236, 274, 397
FaiI YATR 6 cut(s) 234, 272, 375, 377, 395, 414
FaqI GGGAC 1 cut(s) 370
FatI CATG 3 cut(s) 232, 270, 393
FauI CCCGC 1 cut(s) 353
Fnu4HI GCNGC 5 cut(s) 89, 104, 107, 349, 465
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 5 cut(s) 89, 104, 107, 349, 465
GluI GCNGC 5 cut(s) 89, 104, 107, 349, 465
HaeIII GGCC 2 cut(s) 109, 298
HapII CCGG 3 cut(s) 98, 185, 360
Hin1II CATG 3 cut(s) 236, 274, 397
HinfI GANTC 4 cut(s) 39, 94, 114, 152
HpaII CCGG 3 cut(s) 98, 185, 360
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 2 cut(s) 11, 38
Hpy188III TCNNGA 2 cut(s) 98, 394
Hpy8I GTNNAC 1 cut(s) 163
HpyAV CCTTC 3 cut(s) 213, 246, 473
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4V TGCA 4 cut(s) 149, 207, 309, 467
HpyF10VI GCNNNNNNNGC 3 cut(s) 315, 324, 348
HpyF3I CTNAG 1 cut(s) 17
Hsp92II CATG 3 cut(s) 236, 274, 397
Kpn2I TCCGGA 1 cut(s) 97
KspI CCGCGG 2 cut(s) 92, 107
Kzo9I GATC 2 cut(s) 187, 451
LmnI GCTCC 4 cut(s) 70, 100, 285, 347
LpnPI CCDG 7 cut(s) 29, 42, 111, 195, 198, 295, 373
Lsp1109I GCAGC 2 cut(s) 335, 451
MaeIII GTNAC 1 cut(s) 335
MalI GATC 2 cut(s) 189, 453
MboI GATC 2 cut(s) 187, 451
MboII GAAGA 4 cut(s) 71, 190, 258, 409
MfeI CAATTG 1 cut(s) 226
MflI RGATCY 1 cut(s) 187
MhlI GDGCHC 2 cut(s) 23, 67
MlsI TGGCCA 1 cut(s) 298
MluCI AATT 2 cut(s) 226, 259
MluNI TGGCCA 1 cut(s) 298
MlyI GAGTC 2 cut(s) 108, 161
Mox20I TGGCCA 1 cut(s) 298
MroI TCCGGA 1 cut(s) 97
MscI TGGCCA 1 cut(s) 298
MseI TTAA 1 cut(s) 426
MslI CAYNNNNRTG 2 cut(s) 275, 398
Msp20I TGGCCA 1 cut(s) 298
MspA1I CMGCKG 3 cut(s) 91, 106, 348
MspI CCGG 3 cut(s) 98, 185, 360
MunI CAATTG 1 cut(s) 226
MvnI CGCG 2 cut(s) 91, 106
MwoI GCNNNNNNNGC 3 cut(s) 315, 324, 348
NdeII GATC 2 cut(s) 187, 451
NlaIII CATG 3 cut(s) 236, 274, 397
NlaIV GGNNCC 4 cut(s) 102, 110, 189, 358
NspV TTCGAA 1 cut(s) 42
PagI TCATGA 1 cut(s) 393
PfeI GAWTC 2 cut(s) 39, 94
PinAI ACCGGT 1 cut(s) 359
PkrI GCNGC 5 cut(s) 90, 105, 108, 350, 466
PleI GAGTC 2 cut(s) 108, 160
PpsI GAGTC 2 cut(s) 108, 160
Psp124BI GAGCTC 1 cut(s) 23
PspN4I GGNNCC 4 cut(s) 102, 110, 189, 358
PspPI GGNCC 2 cut(s) 108, 357
PstI CTGCAG 1 cut(s) 469
PsuI RGATCY 1 cut(s) 187
RseI CAYNNNNRTG 2 cut(s) 275, 398
SacI GAGCTC 1 cut(s) 23
SacII CCGCGG 2 cut(s) 92, 107
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 5 cut(s) 89, 104, 107, 349, 465
Sau3AI GATC 2 cut(s) 187, 451
Sau96I GGNCC 2 cut(s) 108, 357
SchI GAGTC 2 cut(s) 108, 161
SduI GDGCHC 2 cut(s) 23, 67
SetI ASST 2 cut(s) 23, 204
SfcI CTRYAG 1 cut(s) 465
Sfr303I CCGCGG 2 cut(s) 92, 107
SfuI TTCGAA 1 cut(s) 42
SgrBI CCGCGG 2 cut(s) 92, 107
SinI GGWCC 1 cut(s) 357
SmiMI CAYNNNNRTG 2 cut(s) 275, 398
Sse9I AATT 2 cut(s) 226, 259
SsiI CCGC 9 cut(s) 74, 86, 89, 91, 104, 106, 318, 327, 346
SstI GAGCTC 1 cut(s) 23
StyI CCWWGG 1 cut(s) 352
TaaI ACNGT 1 cut(s) 62
TaqI TCGA 1 cut(s) 42
TasI AATT 2 cut(s) 226, 259
TauI GCSGC 3 cut(s) 91, 106, 109
TfiI GAWTC 2 cut(s) 39, 94
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TscAI CASTG 1 cut(s) 214
TseI GCWGC 2 cut(s) 348, 464
TspDTI ATGAA 3 cut(s) 287, 382, 410
TspGWI ACGGA 1 cut(s) 134
TspRI CASTG 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 357
XapI RAATTY 1 cut(s) 259
XcmI CCANNNNNNNNNTGG 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.