MD02G1232700.v1.1

Protein of unknown function (DUF1005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008694
Physical Location & Seq
Forward (+)
27895826 .. 27899832
4007 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1232700.v1.1.491

Sequence Viewer

Length: 1314 bp
ATGGATCCTCAGGCTTTTATTAGGTTGTCAATAGGCTCACTTGGACTAAGGATTCCTGGAACACCTTTCAAGTCTGAAAAATCTGGCATTCATGCATTATCTTCCCCGTATTCGTGTGAAATTCGCCTTCGAGGGTTCCCTATGCAGACAGCATCAATTCCCTTAGTTTCTTCCCCTGAAGCTACTACTACACCTGATTCTCTCAGCACGGCCTCGAGTTTTTATCTTGAAGAATCGGATATTAAAGCGCTGCTGGAACCTGGCTGTTTCTACAATCCTCATTCCTGTCTGGAGATTGCTATTTTCACAGGGAAGAAGGGATCCCACTGCGGTGTAGGGAACAAAAGGCAGCAAATTGGGACATATAAGCTGGAGGTTGGTCCCGAATGGGGCCAAGGAAAGCCAGTGGTTCTTTTCTGTGGATGGATAGCTATCGGCAAAAACAAGCACGAAAGCGGAAAATTGAGTGCAGAGCTTCATTTGAGAATAAGACTAGATCCCGATCCAAGATATGTTTTCCAGTTTGACGATGTGACAAAACTTAGTCCTCAGGTTGTTCAGCTTCAAGGCTCCAATAAACAGCCTATTTTCAGCTGCAAGTTCATCCGAGACAGGGTTCCCCAGGTGGATCCATTGAACACATACTGGTCAGGTGCTGCTAACAATCCTGATCTGGAAACAGAGAGAAGGGAGAGAAAGGGATGGAAGGTGACAATACATGATCTCTCTGGCTCGGCTGTTGCAGCAGCCTTTATAACAACTCCATTTGTTCCGGCAACAGGTTGTGATTGGGTTGCAAGGTCCAACCCAGGAGCTTGGTTGATTGTTAGTCCTGATCCTTGCAGGCCTGATAGCTGGCAGCCGTGGGGAAAACTTGAAGCATGGCGTGAGCGTGGCATCAGAGACTCTGTTTGCTGCCGATTTCGCCTTCTGTCAGAATGCCAGGAGGCCGTGGAGCTCTTCATGTCTGAAATCCGCATCAATGCTGAAAAGGGTGGGGAGTTTTTCATAGACACAGACAGACAGATGCGGGCAGTAGCAGCGGCAGCAGCATCCGCCAGTCCGATACCAAGCCCACAAAGCAGTGGAGACTTTGCAGGTTTATATCCATTTGTGGGTGGTTTCGTCATGAGCTGTAGAGTGCAAGGGGAAGGAAAAGCCAGCAAGCCATTGGTACAACTAGCCATGAGGCATGTGACATGTGTGGAGGATGCCGCCATCTTCATGGCTCTTGCAGCTGCTGTCGATCTTAGCATTGAGGCATGTAGGCCCTTCCGTCGGAAGTTCAGGAAAACCTCCTGCCCTTCTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1005

Protein Analysis

438

Amino Acids

48.05

Weight (kDa)

8.56

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1005 PF06219 1 - 433 1.5e-178 Protein of unknown function (DUF1005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 755
Acc36I ACCTGC 1 cut(s) 1088
AciI CCGC 7 cut(s) 330, 456, 976, 1030, 1043, 1056, 1215
AclWI GGATC 8 cut(s) 12, 315, 328, 491, 497, 623, 636, 830
AcsI RAATTY 1 cut(s) 120
AcuI CTGAAG 1 cut(s) 198
AfaI GTAC 1 cut(s) 1176
AfeI AGCGCT 1 cut(s) 249
AfiI CCNNNNNNNGG 5 cut(s) 389, 613, 779, 1115, 1278
AflIII ACRYGT 1 cut(s) 1199
AgsI TTSAA 5 cut(s) 70, 230, 566, 637, 878
AjnI CCWGG 5 cut(s) 55, 259, 621, 808, 942
AleI CACNNNNGTG 1 cut(s) 330
Alw21I GWGCWC 1 cut(s) 960
Alw26I GTCTC 3 cut(s) 603, 897, 1083
AlwI GGATC 8 cut(s) 12, 315, 328, 491, 497, 623, 636, 830
AlwNI CAGNNNCTG 2 cut(s) 656, 1241
Ama87I CYCGRG 1 cut(s) 214
Aor51HI AGCGCT 1 cut(s) 249
AoxI GGCC 5 cut(s) 210, 391, 845, 948, 1268
ApoI RAATTY 1 cut(s) 120
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 4 cut(s) 380, 391, 801, 1269
AsuHPI GGTGA 1 cut(s) 721
AvaI CYCGRG 1 cut(s) 214
AvaII GGWCC 2 cut(s) 380, 801
AxyI CCTNAGG 2 cut(s) 9, 549
BamHI GGATCC 3 cut(s) 4, 320, 628
BanII GRGCYC 1 cut(s) 960
Bbv12I GWGCWC 1 cut(s) 960
BccI CCATC 3 cut(s) 417, 696, 1226
BceAI ACGGC 3 cut(s) 225, 847, 935
BciT130I CCWGG 5 cut(s) 57, 261, 623, 810, 944
BcoDI GTCTC 3 cut(s) 603, 897, 1083
BfaI CTAG 2 cut(s) 494, 1181
BfmI CTRYAG 1 cut(s) 1135
BfoI RGCGCY 1 cut(s) 251
BfuAI ACCTGC 1 cut(s) 1088
Bme1390I CCNGG 5 cut(s) 57, 261, 623, 810, 944
Bme18I GGWCC 2 cut(s) 380, 801
BmeT110I CYCGRG 1 cut(s) 214
BmgT120I GGNCC 4 cut(s) 380, 391, 801, 1269
BmiI GGNNCC 9 cut(s) 6, 137, 258, 322, 382, 392, 571, 618, 630
BmrFI CCNGG 5 cut(s) 57, 261, 623, 810, 944
BmsI GCATC 6 cut(s) 161, 906, 987, 1017, 1061, 1201
BpmI CTGGAG 2 cut(s) 311, 392
BsaBI GATNNNNATC 2 cut(s) 431, 501
BsaJI CCNNGG 5 cut(s) 394, 621, 808, 863, 951
Bsc4I CCNNNNNNNGG 5 cut(s) 389, 613, 779, 1115, 1278
Bse1I ACTGG 4 cut(s) 404, 520, 650, 1059
Bse21I CCTNAGG 2 cut(s) 9, 549
Bse8I GATNNNNATC 2 cut(s) 431, 501
BseBI CCWGG 5 cut(s) 57, 261, 623, 810, 944
BseDI CCNNGG 5 cut(s) 394, 621, 808, 863, 951
BseGI GGATG 5 cut(s) 428, 603, 707, 1052, 1216
BseJI GATNNNNATC 2 cut(s) 431, 501
BseLI CCNNNNNNNGG 5 cut(s) 389, 613, 779, 1115, 1278
BseMII CTCAG 3 cut(s) 23, 217, 563
BseNI ACTGG 4 cut(s) 404, 520, 650, 1059
BsgI GTGCAG 1 cut(s) 489
BshFI GGCC 5 cut(s) 212, 393, 847, 950, 1270
BsiHKAI GWGCWC 1 cut(s) 960
BsiHKCI CYCGRG 1 cut(s) 214
BsiSI CCGG 1 cut(s) 773
BslFI GGGAC 2 cut(s) 366, 373
BslI CCNNNNNNNGG 5 cut(s) 389, 613, 779, 1115, 1278
BsmAI GTCTC 3 cut(s) 603, 897, 1083
BsmFI GGGAC 2 cut(s) 366, 373
BsmI GAATGC 2 cut(s) 87, 944
BsnI GGCC 5 cut(s) 212, 393, 847, 950, 1270
BsoBI CYCGRG 1 cut(s) 214
Bsp1286I GDGCHC 1 cut(s) 960
Bsp143I GATC 9 cut(s) 4, 320, 496, 502, 628, 670, 721, 835, 1246
BspACI CCGC 7 cut(s) 330, 456, 976, 1030, 1043, 1056, 1215
BspANI GGCC 5 cut(s) 212, 393, 847, 950, 1270
BspCNI CTCAG 3 cut(s) 22, 216, 562
BspHI TCATGA 1 cut(s) 1128
BspLI GGNNCC 9 cut(s) 6, 137, 258, 322, 382, 392, 571, 618, 630
BspMI ACCTGC 1 cut(s) 1088
BspPI GGATC 8 cut(s) 12, 315, 328, 491, 497, 623, 636, 830
BspQI GCTCTTC 1 cut(s) 965
BsrI ACTGG 4 cut(s) 404, 520, 650, 1059
BssECI CCNNGG 5 cut(s) 394, 621, 808, 863, 951
BssMI GATC 9 cut(s) 4, 320, 496, 502, 628, 670, 721, 835, 1246
BssT1I CCWWGG 1 cut(s) 394
Bst2UI CCWGG 5 cut(s) 57, 261, 623, 810, 944
Bst6I CTCTTC 1 cut(s) 965
BstC8I GCNNGC 5 cut(s) 845, 857, 1032, 1162, 1166
BstDEI CTNAG 7 cut(s) 9, 47, 163, 203, 542, 549, 1250
BstDSI CCRYGG 2 cut(s) 863, 951
BstF5I GGATG 5 cut(s) 428, 603, 707, 1052, 1216
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 9 cut(s) 7, 323, 499, 505, 631, 673, 724, 838, 1249
BstMAI GTCTC 3 cut(s) 603, 897, 1083
BstMBI GATC 9 cut(s) 4, 320, 496, 502, 628, 670, 721, 835, 1246
BstMWI GCNNNNNNNGC 8 cut(s) 743, 924, 1040, 1046, 1049, 1055, 1080, 1235
BstNI CCWGG 5 cut(s) 57, 261, 623, 810, 944
BstNSI RCATGY 3 cut(s) 1196, 1203, 1266
BstSCI CCNGG 5 cut(s) 55, 259, 621, 808, 942
BstSFI CTRYAG 1 cut(s) 1135
BstX2I RGATCY 4 cut(s) 4, 320, 496, 628
BstXI CCANNNNNNTGG 2 cut(s) 816, 1225
BstYI RGATCY 4 cut(s) 4, 320, 496, 628
Bsu36I CCTNAGG 2 cut(s) 9, 549
BsuRI GGCC 5 cut(s) 212, 393, 847, 950, 1270
BtgI CCRYGG 2 cut(s) 863, 951
BtsCI GGATG 5 cut(s) 428, 603, 707, 1052, 1216
BtsI GCAGTG 2 cut(s) 325, 1090
BtsIMutI CAGTG 3 cut(s) 325, 411, 1090
BveI ACCTGC 1 cut(s) 1088
Cac8I GCNNGC 5 cut(s) 845, 857, 1032, 1162, 1166
CaiI CAGNNNCTG 2 cut(s) 656, 1241
CciI TCATGA 1 cut(s) 1128
CfoI GCGC 1 cut(s) 250
Cfr13I GGNCC 4 cut(s) 380, 391, 801, 1269
Csp6I GTAC 1 cut(s) 1175
CviQI GTAC 1 cut(s) 1175
DdeI CTNAG 7 cut(s) 9, 47, 163, 203, 542, 549, 1250
DpnI GATC 9 cut(s) 6, 322, 498, 504, 630, 672, 723, 837, 1248
DpnII GATC 9 cut(s) 4, 320, 496, 502, 628, 670, 721, 835, 1246
Eam1104I CTCTTC 1 cut(s) 965
EarI CTCTTC 1 cut(s) 965
EciI GGCGGA 1 cut(s) 1045
Ecl136II GAGCTC 1 cut(s) 958
Eco130I CCWWGG 1 cut(s) 394
Eco147I AGGCCT 1 cut(s) 847
Eco24I GRGCYC 1 cut(s) 960
Eco47I GGWCC 2 cut(s) 380, 801
Eco47III AGCGCT 1 cut(s) 249
Eco53kI GAGCTC 1 cut(s) 958
Eco57I CTGAAG 1 cut(s) 198
Eco81I CCTNAGG 2 cut(s) 9, 549
Eco88I CYCGRG 1 cut(s) 214
EcoICRI GAGCTC 1 cut(s) 958
EcoO109I RGGNCCY 1 cut(s) 1269
EcoRII CCWGG 5 cut(s) 55, 259, 621, 808, 942
EcoT14I CCWWGG 1 cut(s) 394
EcoT22I ATGCAT 1 cut(s) 97
EcoT38I GRGCYC 1 cut(s) 960
ErhI CCWWGG 1 cut(s) 394
FaqI GGGAC 2 cut(s) 366, 373
FauI CCCGC 1 cut(s) 1023
FokI GGATG 5 cut(s) 435, 590, 714, 1039, 1223
FriOI GRGCYC 1 cut(s) 960
FspBI CTAG 2 cut(s) 494, 1181
GlaI GCGC 1 cut(s) 249
GsuI CTGGAG 2 cut(s) 311, 392
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 5 cut(s) 212, 393, 847, 950, 1270
HapII CCGG 1 cut(s) 773
HhaI GCGC 1 cut(s) 250
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HinfI GANTC 4 cut(s) 52, 197, 233, 905
HpaII CCGG 1 cut(s) 773
HphI GGTGA 1 cut(s) 721
Hpy188I TCNGA 8 cut(s) 76, 238, 608, 902, 937, 970, 1065, 1281
Hpy188III TCNNGA 9 cut(s) 227, 290, 383, 500, 668, 674, 833, 1129, 1288
Hpy99I CGWCG 1 cut(s) 1281
HpyAV CCTTC 8 cut(s) 137, 310, 681, 700, 938, 1145, 1282, 1314
HpyF10VI GCNNNNNNNGC 8 cut(s) 743, 924, 1040, 1046, 1049, 1055, 1080, 1235
HpyF3I CTNAG 7 cut(s) 9, 47, 163, 203, 542, 549, 1250
HspAI GCGC 1 cut(s) 248
Kzo9I GATC 9 cut(s) 4, 320, 496, 502, 628, 670, 721, 835, 1246
LguI GCTCTTC 1 cut(s) 965
LmnI GCTCC 3 cut(s) 575, 812, 955
LweI GCATC 6 cut(s) 161, 906, 987, 1017, 1061, 1201
MaeI CTAG 2 cut(s) 494, 1181
MaeIII GTNAC 3 cut(s) 532, 709, 1195
MalI GATC 9 cut(s) 6, 322, 498, 504, 630, 672, 723, 837, 1248
MboI GATC 9 cut(s) 4, 320, 496, 502, 628, 670, 721, 835, 1246
MboII GAAGA 6 cut(s) 93, 162, 242, 325, 952, 1213
MflI RGATCY 4 cut(s) 4, 320, 496, 628
MhlI GDGCHC 1 cut(s) 960
MluCI AATT 4 cut(s) 120, 156, 354, 461
MlyI GAGTC 1 cut(s) 899
MmeI TCCRAC 2 cut(s) 828, 1259
Mph1103I ATGCAT 1 cut(s) 97
MseI TTAA 1 cut(s) 243
MslI CAYNNNNRTG 2 cut(s) 330, 1223
MspA1I CMGCKG 3 cut(s) 594, 1043, 1238
MspI CCGG 1 cut(s) 773
MspR9I CCNGG 5 cut(s) 57, 261, 623, 810, 944
Mva1269I GAATGC 2 cut(s) 87, 944
MvaI CCWGG 5 cut(s) 57, 261, 623, 810, 944
MwoI GCNNNNNNNGC 8 cut(s) 743, 924, 1040, 1046, 1049, 1055, 1080, 1235
NdeII GATC 9 cut(s) 4, 320, 496, 502, 628, 670, 721, 835, 1246
NlaIV GGNNCC 9 cut(s) 6, 137, 258, 322, 382, 392, 571, 618, 630
NmeAIII GCCGAG 1 cut(s) 713
NmuCI GTSAC 3 cut(s) 532, 709, 1195
NsiI ATGCAT 1 cut(s) 97
NspI RCATGY 3 cut(s) 1196, 1203, 1266
OliI CACNNNNGTG 1 cut(s) 330
PaeR7I CTCGAG 1 cut(s) 214
PagI TCATGA 1 cut(s) 1128
PceI AGGCCT 1 cut(s) 847
PciI ACATGT 1 cut(s) 1199
PciSI GCTCTTC 1 cut(s) 965
PctI GAATGC 2 cut(s) 87, 944
PfeI GAWTC 3 cut(s) 52, 197, 233
PfoI TCCNGGA 1 cut(s) 55
PleI GAGTC 1 cut(s) 899
PpsI GAGTC 1 cut(s) 899
PscI ACATGT 1 cut(s) 1199
PsiI TTATAA 1 cut(s) 755
Psp124BI GAGCTC 1 cut(s) 960
Psp6I CCWGG 5 cut(s) 55, 259, 621, 808, 942
PspGI CCWGG 5 cut(s) 55, 259, 621, 808, 942
PspN4I GGNNCC 9 cut(s) 6, 137, 258, 322, 382, 392, 571, 618, 630
PspPI GGNCC 4 cut(s) 380, 391, 801, 1269
PspXI VCTCGAGB 1 cut(s) 214
PstNI CAGNNNCTG 2 cut(s) 656, 1241
PsuI RGATCY 4 cut(s) 4, 320, 496, 628
PvuII CAGCTG 2 cut(s) 594, 1238
RsaI GTAC 1 cut(s) 1176
RsaNI GTAC 1 cut(s) 1175
RseI CAYNNNNRTG 2 cut(s) 330, 1223
SacI GAGCTC 1 cut(s) 960
SapI GCTCTTC 1 cut(s) 965
SaqAI TTAA 1 cut(s) 243
Sau3AI GATC 9 cut(s) 4, 320, 496, 502, 628, 670, 721, 835, 1246
Sau96I GGNCC 4 cut(s) 380, 391, 801, 1269
SchI GAGTC 1 cut(s) 899
ScrFI CCNGG 5 cut(s) 57, 261, 623, 810, 944
SduI GDGCHC 1 cut(s) 960
SfaNI GCATC 6 cut(s) 161, 906, 987, 1017, 1061, 1201
SfcI CTRYAG 1 cut(s) 1135
Sfr274I CTCGAG 1 cut(s) 214
SinI GGWCC 2 cut(s) 380, 801
SlaI CTCGAG 1 cut(s) 214
SmiMI CAYNNNNRTG 2 cut(s) 330, 1223
SmlI CTYRAG 1 cut(s) 214
SmoI CTYRAG 1 cut(s) 214
Sse9I AATT 4 cut(s) 120, 156, 354, 461
SseBI AGGCCT 1 cut(s) 847
SsiI CCGC 7 cut(s) 330, 456, 976, 1030, 1043, 1056, 1215
SspMI CTAG 2 cut(s) 494, 1181
SstI GAGCTC 1 cut(s) 960
StuI AGGCCT 1 cut(s) 847
StyD4I CCNGG 5 cut(s) 55, 259, 621, 808, 942
StyI CCWWGG 1 cut(s) 394
TaqI TCGA 3 cut(s) 130, 215, 1245
TasI AATT 4 cut(s) 120, 156, 354, 461
TauI GCSGC 2 cut(s) 1046, 1217
TfiI GAWTC 3 cut(s) 52, 197, 233
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TscAI CASTG 3 cut(s) 332, 411, 1090
TseFI GTSAC 3 cut(s) 532, 709, 1195
Tsp45I GTSAC 3 cut(s) 532, 709, 1195
TspDTI ATGAA 6 cut(s) 80, 467, 592, 952, 997, 1213
TspGWI ACGGA 1 cut(s) 1265
TspRI CASTG 3 cut(s) 332, 411, 1090
VpaK11BI GGWCC 2 cut(s) 380, 801
XapI RAATTY 1 cut(s) 120
XceI RCATGY 3 cut(s) 1196, 1203, 1266
XcmI CCANNNNNNNNNTGG 1 cut(s) 1168
XhoI CTCGAG 1 cut(s) 214
XspI CTAG 2 cut(s) 494, 1181
Zsp2I ATGCAT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.