MD07G1081000.v1.1

Protein of unknown function (DUF1005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008694
Physical Location & Seq
Reverse (-)
7851925 .. 7854038
2114 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1081000.v1.1.491

Sequence Viewer

Length: 1305 bp
ATGGATCCTCAGGCTTTCATTAGGTTGTCAATAGGCTCACTTGGACTAAGGATTCCTGGAACACCTTTAAAGTCTGAAAAATCTGGTATTCATGCATTCTCTTCCCCATATTCGTGTGAAATTCGCCTTCGAGGATTCCCTATGCAGACAGCATCAATTCCCTTAGTATCTTCTCCTGAAGCTGCTACACCTGATTCTCATAGCACTGCCTCCAGTTTTTATCTTGAAAAATCTGATATTAAAGCACTGCTGGAACCCGGCTGTTTCTACAATCCTCATTCCTGTCTGGAGATCGCTATTTTCACTGGGAAGAAGGGATCCCACTGTGGTGTAGGGACCAAAAGGCAGCAAATTGGGACATATAAGCTTGAGGTTGGTCCTGAATGGGGGGAAGGAAAGTCAGGGGTTCTTTTCAGTGGGTGGATAGCAATCGGCAAAAACAAGCAGGAGAATGGAAAATTGAGTGCGGAGCTTCATTTGAGAGTAAGATTAGATCCTGATCCGAGATATGTTTTCCAGTTTGATGATGTGACAAAACTTAGTCCTCAGATAGTTCAGCTTCAAGGCTCCATTAAACAACCTATTTTCAGCTGCAAGTTCATCCGAGACAGGGTTCCCCAGGTGGATCCATTGAGCACATACTGGTCAGGTGCTGCTAACAATCCTGATCTGGATACAGAGAGAAGAGAGAGAAGGGGATGGAAGGTGACAATACATGATCTCTCTGGCTCGGCTATTGCAGCGGCCTTTATAACAACCCCATTTGTTCCAGCAACAGGATGTGATTGGGTTGCAAGGTCCAACCCAGGAGCTTGGTTGATTGTTAGTCCTGATGCTTGCAGGCCTGATAGCTGGCAGCCATGGGGAAAGCTTGAGGCATGGCGTGAGCGTGGCATCAGAGACTCCGTTTGCTGTCGATTTCGCCTTCTGTCAGAATGCCAGGAGGCTGTGGACCTCTTCATGTCTGAAATCCGTATCAATGCTGAAAAGGGTGGAGAGTTTTCCATAGACACAGACAAACAGATGCAGGCAGCAGCGGCAACAGCAGCCGCCAGTCCGATACCAAGCCCACAAACCAGTGGAGACTTTGCAGGTTTGTATCCAGATGTTGGTGGTTTCGTCATGAGCTGTAGAGTGCAAGGGGAAGGAAAAGCCAGCAAGCCATTGGTACAACTAGCAATGAGGCATGTGACTTGTGTGGAGGATGCCGCCATCTTCATGGCTCTTGCAGCTGCAGTTGATCTCAGCATAGAGGCATGTAGGCCCTTCCGGAAGTTGAGGAAACCCTCCTGCCCTTCTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1005

Protein Analysis

435

Amino Acids

47.43

Weight (kDa)

8.33

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1005 PF06219 1 - 429 5.8e-177 Protein of unknown function (DUF1005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 752
Acc36I ACCTGC 1 cut(s) 1082
AccB7I CCANNNNNTGG 1 cut(s) 1109
AccIII TCCGGA 1 cut(s) 1269
AciI CCGC 5 cut(s) 467, 743, 1037, 1050, 1209
AclWI GGATC 7 cut(s) 12, 312, 325, 488, 494, 620, 633
AcsI RAATTY 1 cut(s) 120
AcuI CTGAAG 1 cut(s) 198
AfaI GTAC 1 cut(s) 1170
AfiI CCNNNNNNNGG 4 cut(s) 386, 610, 776, 1109
AgsI TTSAA 2 cut(s) 227, 563
AjnI CCWGG 4 cut(s) 55, 618, 805, 939
AleI CACNNNNGTG 1 cut(s) 327
Alw21I GWGCWC 1 cut(s) 638
Alw26I GTCTC 3 cut(s) 600, 894, 1077
AlwI GGATC 7 cut(s) 12, 312, 325, 488, 494, 620, 633
AlwNI CAGNNNCTG 1 cut(s) 653
Aor13HI TCCGGA 1 cut(s) 1269
AoxI GGCC 3 cut(s) 744, 842, 1262
ApoI RAATTY 1 cut(s) 120
AspS9I GGNCC 5 cut(s) 336, 377, 798, 952, 1263
AsuC2I CCSGG 1 cut(s) 258
AsuHPI GGTGA 1 cut(s) 718
AvaII GGWCC 4 cut(s) 336, 377, 798, 952
AxyI CCTNAGG 1 cut(s) 9
BamHI GGATCC 3 cut(s) 4, 317, 625
Bbv12I GWGCWC 1 cut(s) 638
BccI CCATC 2 cut(s) 693, 1220
BciT130I CCWGG 4 cut(s) 57, 620, 807, 941
BciVI GTATCC 2 cut(s) 667, 1110
BcnI CCSGG 1 cut(s) 258
BcoDI GTCTC 3 cut(s) 600, 894, 1077
BfaI CTAG 1 cut(s) 1175
BfmI CTRYAG 2 cut(s) 1129, 1233
BfuAI ACCTGC 1 cut(s) 1082
BfuI GTATCC 2 cut(s) 667, 1110
Bme1390I CCNGG 5 cut(s) 57, 258, 620, 807, 941
Bme18I GGWCC 4 cut(s) 336, 377, 798, 952
BmgT120I GGNCC 5 cut(s) 336, 377, 798, 952, 1263
BmiI GGNNCC 7 cut(s) 6, 255, 319, 337, 568, 615, 627
BmrFI CCNGG 5 cut(s) 57, 258, 620, 807, 941
BmrI ACTGGG 1 cut(s) 315
BmsI GCATC 5 cut(s) 161, 823, 903, 1014, 1195
BmuI ACTGGG 1 cut(s) 315
BpmI CTGGAG 2 cut(s) 196, 308
BpuEI CTTGAG 2 cut(s) 389, 893
BpuMI CCSGG 1 cut(s) 258
BsaBI GATNNNNATC 2 cut(s) 428, 498
BsaJI CCNNGG 3 cut(s) 618, 805, 860
BsaWI WCCGGW 1 cut(s) 1269
Bsc4I CCNNNNNNNGG 4 cut(s) 386, 610, 776, 1109
Bse1I ACTGG 6 cut(s) 213, 310, 517, 647, 1053, 1077
Bse21I CCTNAGG 1 cut(s) 9
Bse3DI GCAATG 1 cut(s) 1185
Bse8I GATNNNNATC 2 cut(s) 428, 498
BseAI TCCGGA 1 cut(s) 1269
BseBI CCWGG 4 cut(s) 57, 620, 807, 941
BseDI CCNNGG 3 cut(s) 618, 805, 860
BseGI GGATG 4 cut(s) 600, 704, 785, 1210
BseJI GATNNNNATC 2 cut(s) 428, 498
BseLI CCNNNNNNNGG 4 cut(s) 386, 610, 776, 1109
BseMI GCAATG 1 cut(s) 1185
BseMII CTCAG 3 cut(s) 23, 560, 1258
BseNI ACTGG 6 cut(s) 213, 310, 517, 647, 1053, 1077
BshFI GGCC 3 cut(s) 746, 844, 1264
BsiHKAI GWGCWC 1 cut(s) 638
BsiSI CCGG 2 cut(s) 258, 1270
BslFI GGGAC 2 cut(s) 349, 370
BslI CCNNNNNNNGG 4 cut(s) 386, 610, 776, 1109
BsmAI GTCTC 3 cut(s) 600, 894, 1077
BsmFI GGGAC 2 cut(s) 349, 370
BsmI GAATGC 2 cut(s) 95, 941
BsnI GGCC 3 cut(s) 746, 844, 1264
Bsp1286I GDGCHC 1 cut(s) 638
Bsp13I TCCGGA 1 cut(s) 1269
Bsp143I GATC 9 cut(s) 4, 291, 317, 493, 499, 625, 667, 718, 1240
Bsp19I CCATGG 1 cut(s) 860
BspACI CCGC 5 cut(s) 467, 743, 1037, 1050, 1209
BspANI GGCC 3 cut(s) 746, 844, 1264
BspCNI CTCAG 3 cut(s) 22, 559, 1257
BspEI TCCGGA 1 cut(s) 1269
BspHI TCATGA 1 cut(s) 1122
BspLI GGNNCC 7 cut(s) 6, 255, 319, 337, 568, 615, 627
BspMAI CTGCAG 1 cut(s) 1237
BspMI ACCTGC 1 cut(s) 1082
BspPI GGATC 7 cut(s) 12, 312, 325, 488, 494, 620, 633
BsrDI GCAATG 1 cut(s) 1185
BsrI ACTGG 6 cut(s) 213, 310, 517, 647, 1053, 1077
BssECI CCNNGG 3 cut(s) 618, 805, 860
BssMI GATC 9 cut(s) 4, 291, 317, 493, 499, 625, 667, 718, 1240
BssT1I CCWWGG 1 cut(s) 860
Bst2UI CCWGG 4 cut(s) 57, 620, 807, 941
Bst4CI ACNGT 1 cut(s) 326
Bst6I CTCTTC 3 cut(s) 106, 679, 962
BstC8I GCNNGC 6 cut(s) 838, 842, 854, 1029, 1156, 1160
BstDEI CTNAG 6 cut(s) 9, 47, 163, 539, 546, 1244
BstDSI CCRYGG 1 cut(s) 860
BstF5I GGATG 4 cut(s) 600, 704, 785, 1210
BstKTI GATC 9 cut(s) 7, 294, 320, 496, 502, 628, 670, 721, 1243
BstMAI GTCTC 3 cut(s) 600, 894, 1077
BstMBI GATC 9 cut(s) 4, 291, 317, 493, 499, 625, 667, 718, 1240
BstMWI GCNNNNNNNGC 5 cut(s) 740, 1037, 1043, 1046, 1229
BstNI CCWGG 4 cut(s) 57, 620, 807, 941
BstNSI RCATGY 2 cut(s) 1190, 1260
BstSCI CCNGG 5 cut(s) 55, 256, 618, 805, 939
BstSFI CTRYAG 2 cut(s) 1129, 1233
BstX2I RGATCY 4 cut(s) 4, 317, 493, 625
BstXI CCANNNNNNTGG 2 cut(s) 813, 1219
BstYI RGATCY 4 cut(s) 4, 317, 493, 625
Bsu36I CCTNAGG 1 cut(s) 9
BsuI GTATCC 2 cut(s) 667, 1110
BsuRI GGCC 3 cut(s) 746, 844, 1264
BtgI CCRYGG 1 cut(s) 860
BtsCI GGATG 4 cut(s) 600, 704, 785, 1210
BtsI GCAGTG 2 cut(s) 204, 245
BtsIMutI CAGTG 6 cut(s) 204, 245, 303, 322, 421, 1084
BveI ACCTGC 1 cut(s) 1082
Cac8I GCNNGC 6 cut(s) 838, 842, 854, 1029, 1156, 1160
CaiI CAGNNNCTG 1 cut(s) 653
CciI TCATGA 1 cut(s) 1122
Cfr13I GGNCC 5 cut(s) 336, 377, 798, 952, 1263
Csp6I GTAC 1 cut(s) 1169
CviAII CATG 9 cut(s) 92, 716, 861, 879, 961, 1123, 1187, 1219, 1257
CviQI GTAC 1 cut(s) 1169
DdeI CTNAG 6 cut(s) 9, 47, 163, 539, 546, 1244
DpnI GATC 9 cut(s) 6, 293, 319, 495, 501, 627, 669, 720, 1242
DpnII GATC 9 cut(s) 4, 291, 317, 493, 499, 625, 667, 718, 1240
DraI TTTAAA 1 cut(s) 69
Eam1104I CTCTTC 3 cut(s) 106, 679, 962
EarI CTCTTC 3 cut(s) 106, 679, 962
Eco130I CCWWGG 1 cut(s) 860
Eco147I AGGCCT 1 cut(s) 844
Eco47I GGWCC 4 cut(s) 336, 377, 798, 952
Eco57I CTGAAG 1 cut(s) 198
Eco81I CCTNAGG 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 1263
EcoRII CCWGG 4 cut(s) 55, 618, 805, 939
EcoT14I CCWWGG 1 cut(s) 860
EcoT22I ATGCAT 1 cut(s) 97
ErhI CCWWGG 1 cut(s) 860
FaeI CATG 9 cut(s) 95, 719, 864, 882, 964, 1126, 1190, 1222, 1260
FaqI GGGAC 2 cut(s) 349, 370
FatI CATG 9 cut(s) 91, 715, 860, 878, 960, 1122, 1186, 1218, 1256
FokI GGATG 4 cut(s) 587, 711, 792, 1217
FspBI CTAG 1 cut(s) 1175
GsuI CTGGAG 2 cut(s) 196, 308
HaeIII GGCC 3 cut(s) 746, 844, 1264
HapII CCGG 2 cut(s) 258, 1270
Hin1II CATG 9 cut(s) 95, 719, 864, 882, 964, 1126, 1190, 1222, 1260
HindIII AAGCTT 2 cut(s) 365, 869
HinfI GANTC 4 cut(s) 52, 135, 194, 902
HpaII CCGG 2 cut(s) 258, 1270
HphI GGTGA 1 cut(s) 718
Hpy166II GTNNAC 1 cut(s) 952
Hpy188I TCNGA 9 cut(s) 76, 235, 504, 549, 605, 899, 934, 967, 1059
Hpy8I GTNNAC 1 cut(s) 952
HpyAV CCTTC 9 cut(s) 137, 307, 386, 687, 697, 935, 1139, 1276, 1305
HpyCH4III ACNGT 1 cut(s) 326
HpyF10VI GCNNNNNNNGC 5 cut(s) 740, 1037, 1043, 1046, 1229
HpyF3I CTNAG 6 cut(s) 9, 47, 163, 539, 546, 1244
Hsp92II CATG 9 cut(s) 95, 719, 864, 882, 964, 1126, 1190, 1222, 1260
Kpn2I TCCGGA 1 cut(s) 1269
Kzo9I GATC 9 cut(s) 4, 291, 317, 493, 499, 625, 667, 718, 1240
LmnI GCTCC 3 cut(s) 469, 572, 809
LweI GCATC 5 cut(s) 161, 823, 903, 1014, 1195
MaeI CTAG 1 cut(s) 1175
MaeIII GTNAC 3 cut(s) 529, 706, 1189
MalI GATC 9 cut(s) 6, 293, 319, 495, 501, 627, 669, 720, 1242
MboI GATC 9 cut(s) 4, 291, 317, 493, 499, 625, 667, 718, 1240
MboII GAAGA 6 cut(s) 93, 162, 322, 696, 949, 1207
MflI RGATCY 4 cut(s) 4, 317, 493, 625
MhlI GDGCHC 1 cut(s) 638
MluCI AATT 4 cut(s) 120, 156, 351, 458
MlyI GAGTC 1 cut(s) 896
MmeI TCCRAC 1 cut(s) 825
Mph1103I ATGCAT 1 cut(s) 97
MroI TCCGGA 1 cut(s) 1269
MseI TTAA 3 cut(s) 68, 240, 573
MslI CAYNNNNRTG 3 cut(s) 112, 327, 1217
MspA1I CMGCKG 4 cut(s) 591, 743, 1037, 1232
MspI CCGG 2 cut(s) 258, 1270
MspR9I CCNGG 5 cut(s) 57, 258, 620, 807, 941
Mva1269I GAATGC 2 cut(s) 95, 941
MvaI CCWGG 4 cut(s) 57, 620, 807, 941
MwoI GCNNNNNNNGC 5 cut(s) 740, 1037, 1043, 1046, 1229
NciI CCSGG 1 cut(s) 258
NcoI CCATGG 1 cut(s) 860
NdeII GATC 9 cut(s) 4, 291, 317, 493, 499, 625, 667, 718, 1240
NlaIII CATG 9 cut(s) 95, 719, 864, 882, 964, 1126, 1190, 1222, 1260
NlaIV GGNNCC 7 cut(s) 6, 255, 319, 337, 568, 615, 627
NmeAIII GCCGAG 1 cut(s) 710
NmuCI GTSAC 3 cut(s) 529, 706, 1189
NsiI ATGCAT 1 cut(s) 97
NspI RCATGY 2 cut(s) 1190, 1260
OliI CACNNNNGTG 1 cut(s) 327
PagI TCATGA 1 cut(s) 1122
PceI AGGCCT 1 cut(s) 844
PctI GAATGC 2 cut(s) 95, 941
PfeI GAWTC 3 cut(s) 52, 135, 194
PflMI CCANNNNNTGG 1 cut(s) 1109
PfoI TCCNGGA 1 cut(s) 55
PleI GAGTC 1 cut(s) 896
PpsI GAGTC 1 cut(s) 896
PsiI TTATAA 1 cut(s) 752
Psp6I CCWGG 4 cut(s) 55, 618, 805, 939
PspGI CCWGG 4 cut(s) 55, 618, 805, 939
PspN4I GGNNCC 7 cut(s) 6, 255, 319, 337, 568, 615, 627
PspPI GGNCC 5 cut(s) 336, 377, 798, 952, 1263
PstI CTGCAG 1 cut(s) 1237
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 4 cut(s) 4, 317, 493, 625
PvuII CAGCTG 2 cut(s) 591, 1232
RsaI GTAC 1 cut(s) 1170
RsaNI GTAC 1 cut(s) 1169
RseI CAYNNNNRTG 3 cut(s) 112, 327, 1217
SaqAI TTAA 3 cut(s) 68, 240, 573
Sau3AI GATC 9 cut(s) 4, 291, 317, 493, 499, 625, 667, 718, 1240
Sau96I GGNCC 5 cut(s) 336, 377, 798, 952, 1263
SchI GAGTC 1 cut(s) 896
ScrFI CCNGG 5 cut(s) 57, 258, 620, 807, 941
SduI GDGCHC 1 cut(s) 638
SfaNI GCATC 5 cut(s) 161, 823, 903, 1014, 1195
SfcI CTRYAG 2 cut(s) 1129, 1233
SinI GGWCC 4 cut(s) 336, 377, 798, 952
SmiMI CAYNNNNRTG 3 cut(s) 112, 327, 1217
SmlI CTYRAG 2 cut(s) 368, 872
SmoI CTYRAG 2 cut(s) 368, 872
Sse9I AATT 4 cut(s) 120, 156, 351, 458
SseBI AGGCCT 1 cut(s) 844
SsiI CCGC 5 cut(s) 467, 743, 1037, 1050, 1209
SspMI CTAG 1 cut(s) 1175
StuI AGGCCT 1 cut(s) 844
StyD4I CCNGG 5 cut(s) 55, 256, 618, 805, 939
StyI CCWWGG 1 cut(s) 860
TaaI ACNGT 1 cut(s) 326
TaqI TCGA 2 cut(s) 130, 916
TasI AATT 4 cut(s) 120, 156, 351, 458
TauI GCSGC 4 cut(s) 746, 1040, 1052, 1211
TfiI GAWTC 3 cut(s) 52, 135, 194
Tru1I TTAA 3 cut(s) 68, 240, 573
Tru9I TTAA 3 cut(s) 68, 240, 573
TscAI CASTG 6 cut(s) 211, 252, 310, 329, 421, 1084
TseFI GTSAC 3 cut(s) 529, 706, 1189
Tsp45I GTSAC 3 cut(s) 529, 706, 1189
TspDTI ATGAA 6 cut(s) 7, 80, 464, 589, 949, 1207
TspGWI ACGGA 2 cut(s) 895, 962
TspRI CASTG 6 cut(s) 211, 252, 310, 329, 421, 1084
Van91I CCANNNNNTGG 1 cut(s) 1109
VpaK11BI GGWCC 4 cut(s) 336, 377, 798, 952
XapI RAATTY 1 cut(s) 120
XceI RCATGY 2 cut(s) 1190, 1260
XcmI CCANNNNNNNNNTGG 1 cut(s) 1162
XspI CTAG 1 cut(s) 1175
Zsp2I ATGCAT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.