Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0000973

Physical Location & Seq

Genomic Coordinates Forward (+)

32466776 .. 32475880

Gene Structure 9105 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1269900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1575 bp

ATGGACGTGTTCAGACCAAAAAAAAGAAGCAATGTTGTTTGCAGGATTTTCGACCGCCAAATTTCGTCTAGTGCTTCTGGAACCAGTGTTCGCTATACTAGGCGATTTTACGAGAATGTGGTCCCTGATTATACCATATATGAAGTTGAGTGCCCAGATCAGATTTACCGTAAATTCACTGATGATGGACAATACCTAATATGTTTCAGCAGAAATCATCAAGATCTGATTGTTTATAGACCAACATGGCTTTCATATTCATGCAAAGAAGATTGCAATACTAATGATCTTCCCCCAAAAGCAAAGAAGTTTGAGAGCTTCTTTAGCCAGTTATACTGTGTATCACTTGCTTCTAGCAACGATCTTATATGCAAAGACTTTTTTCTTTACATGGGGAGTAACCAATTTGGAATATTTGCTACTTCTACTGCACAAATTCATGATGCACTCCCTGTTGGAGGAGCTGTTCAGGGAGTCCCATCGATAGAAAAGATAACATTTCACCTCTTGAGATTGGAGGATGGAGTTGTTCTGGATCAGAAGTCCTTTCAGAATGATTTTATTAATCTGGCCCATAATATGGGTGTCTTCTTGTATGACGATTTACTGGCGATTGTGTCTCTTCGCTATCAGACAATACACATTCTGCAAATCAGGGATTCTGGCAACCTTGTTGATGTACGATCAATTGGTACATTTTGTCGTGAAGATGATGTGCTTTTTCTCACTTCAAATACTCAGACTGATTTACATCAGGGCCAGCCTTCTTCAGATTATTGTTTTCTTAGCGGAATCAAACAACGGCTGCTATCTTTCATATTTAGAGGAATATGGAATGAAGAATCAGATCAGTCATCGAGGGTCCAATTGCTGAAGAAGAAGTTCTATTTCCATTTTCAAGACTATGTTGACTTGATTATCTGGAAGGTACAGTTCTTGGACCGGCACCACCTGCTAATAAAGTTTGGTAGTGTAGATGGAGTGGTATCTCGCAATGTAGATCATCATCGATCCTTTTTTGCAGTATATAACATGGAGACAACTGAAATTGTAGCTTTTTATCAGAATTCGGCAGATGAGCTTTATCTCTTGTTTGAGCAATTCGGCGACCACTTCCATGCAACCTCGAGAAATTCATTGTATTTAAATTTCACATCATCTCACTCAAATAACATGCATGCTCGAGAACAACTCAGAAGTAATAAAAATAAAGCAAGTAGCTCTTCAGCCTCTTCACAGTTTGTGAAGAAGGTACTAGCCTCTTTGCCTTTTAGTTGTCAGTCGGAGAGCCCTTCTCCCTACTTTGACCAATCACTTTTTAAGTTTGATGAAAAGTCAATTTCTCCAATGGATCGGCATAGACCCTCACCAGATCAACACATAAAGTTCTGCTTAAGAAGGAAACCAAAAGTTCTCAAATTTAAGATGAAACCAGGTCCAGAAGCTGGTAGCACAGACAATCGGACTAAAAGGATCTCTTCGTTTCTTTTTCATCCATTTTTGCCTCTTGCTCTGTCCATCCAGCAGAGTATGTCCTTGCAGCCATCAGTTTTGAATATTCACTTCCGGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009987 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

Det1

Protein Analysis

525

Amino Acids

60.65

Weight (kDa)

8.7

Isoelectric Point (pI)

52.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Det1	PF09737	125 - 524	4.8e-146	De-etiolated protein 1 Det1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0000973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10180
fragaria_vesca	FvH4_7g04420 FvH4_7g04420 FvH4_7g04420 FvH4_7g04420 FvH4_7g04420 FvH4_7g04420 FvH4_7g04420 FvH4_7g04420 FvH4_7g04420 FvH4_7g04420 FvH4_7g04420
malus_domestica	MD02G1269900.v1.1
prunus_persica	Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1 Prupe.2G049900_v2.0.a1
pyrus_communis	pycom02g23100 pycom02g23110
rosa_chinensis	RchiOBHm_Chr1g0329651
rosa_laevigata	RLG00000029875
rosa_multiflora	Rmu_sc0004805.1_g000003 Rmu_sc0016170.1_g000019
rosa_roxburghii	Rroxscaffold_4G00320810
rosa_rugosa	Rorug01G0081800
rosa_samantha	Rh1AG100200 Rh1BG079500 Rh1CG095800 Rh1DG102300
rosa_wichuraiana	Rw1G007790 Rw1G007860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	960
Acc36I	ACCTGC	1 cut(s)	960
AccB1I	GGYRCC	1 cut(s)	945
AccB7I	CCANNNNNTGG	2 cut(s)	580, 1445
AccIII	TCCGGA	1 cut(s)	1566
AciI	CCGC	2 cut(s)	55, 789
AclWI	GGATC	4 cut(s)	543, 1005, 1359, 1481
AcsI	RAATTY	7 cut(s)	60, 173, 435, 1066, 1132, 1147, 1418
AcuI	CTGAAG	3 cut(s)	753, 893, 1209
AfaI	GTAC	4 cut(s)	681, 694, 930, 1254
AfiI	CCNNNNNNNGG	3 cut(s)	458, 580, 1445
AflII	CTTAAG	1 cut(s)	1393
AflIII	ACRYGT	1 cut(s)	6
AgsI	TTSAA	3 cut(s)	732, 899, 1555
AjiI	CACGTC	1 cut(s)	7
AjnI	CCWGG	1 cut(s)	1432
AjuI	GAANNNNNNNTTGG	2 cut(s)	235, 267
AloI	GAACNNNNNNTCC	4 cut(s)	72, 104, 450, 482
AluBI	AGCT	6 cut(s)	318, 464, 1055, 1081, 1221, 1445
AluI	AGCT	6 cut(s)	318, 464, 1055, 1081, 1221, 1445
Alw26I	GTCTC	2 cut(s)	624, 1031
AlwI	GGATC	4 cut(s)	543, 1005, 1359, 1481
AlwNI	CAGNNNCTG	1 cut(s)	1445
Ama87I	CYCGRG	2 cut(s)	1126, 1182
Aor13HI	TCCGGA	1 cut(s)	1566
AoxI	GGCC	2 cut(s)	570, 757
ApeKI	GCWGC	2 cut(s)	805, 1540
ApoI	RAATTY	7 cut(s)	60, 173, 435, 1066, 1132, 1147, 1418
AseI	ATTAAT	1 cut(s)	564
Asp700I	GAANNNNTTC	1 cut(s)	881
AspS9I	GGNCC	6 cut(s)	121, 571, 757, 862, 940, 1436
AsuHPI	GGTGA	2 cut(s)	494, 1359
AvaI	CYCGRG	2 cut(s)	1126, 1182
AvaII	GGWCC	4 cut(s)	121, 862, 940, 1436
BaeGI	GKGCMC	1 cut(s)	155
BanI	GGYRCC	1 cut(s)	945
BanII	GRGCYC	1 cut(s)	1292
BbsI	GAAGAC	1 cut(s)	580
BbvI	GCAGC	2 cut(s)	792, 1552
BccI	CCATC	6 cut(s)	179, 487, 515, 971, 1526, 1552
BceAI	ACGGC	1 cut(s)	818
BcgI	CGANNNNNNTGC	2 cut(s)	31, 65
BciT130I	CCWGG	1 cut(s)	1434
BcoDI	GTCTC	2 cut(s)	624, 1031
BfaI	CTAG	4 cut(s)	69, 99, 354, 1256
BfrI	CTTAAG	1 cut(s)	1393
BfuAI	ACCTGC	1 cut(s)	960
BglII	AGATCT	1 cut(s)	223
BisI	GCNGC	2 cut(s)	806, 1541
BlsI	GCNGC	2 cut(s)	807, 1542
Bme1390I	CCNGG	1 cut(s)	1434
Bme18I	GGWCC	4 cut(s)	121, 862, 940, 1436
BmeT110I	CYCGRG	2 cut(s)	1126, 1182
BmgBI	CACGTC	1 cut(s)	7
BmgT120I	GGNCC	6 cut(s)	121, 571, 757, 862, 940, 1436
BmiI	GGNNCC	4 cut(s)	82, 123, 863, 947
BmrFI	CCNGG	1 cut(s)	1434
BmsI	GCATC	1 cut(s)	433
BpiI	GAAGAC	1 cut(s)	580
BplI	GAGNNNNNCTC	4 cut(s)	1176, 1208, 1279, 1311
BpuEI	CTTGAG	1 cut(s)	529
Bsa29I	ATCGAT	2 cut(s)	482, 1009
BsaBI	GATNNNNATC	2 cut(s)	750, 1005
BsaWI	WCCGGW	1 cut(s)	1566
BsaXI	ACNNNNNCTCC	2 cut(s)	450, 480
Bsc4I	CCNNNNNNNGG	3 cut(s)	458, 580, 1445
Bse118I	RCCGGY	1 cut(s)	942
Bse1I	ACTGG	3 cut(s)	84, 328, 612
Bse3DI	GCAATG	2 cut(s)	37, 1000
Bse8I	GATNNNNATC	2 cut(s)	750, 1005
BseAI	TCCGGA	1 cut(s)	1566
BseBI	CCWGG	1 cut(s)	1434
BseCI	ATCGAT	2 cut(s)	482, 1009
BseGI	GGATG	3 cut(s)	526, 1492, 1518
BseJI	GATNNNNATC	2 cut(s)	750, 1005
BseLI	CCNNNNNNNGG	3 cut(s)	458, 580, 1445
BseMI	GCAATG	2 cut(s)	37, 1000
BseMII	CTCAG	2 cut(s)	752, 1207
BseNI	ACTGG	3 cut(s)	84, 328, 612
BseRI	GAGGAG	1 cut(s)	474
BseSI	GKGCMC	1 cut(s)	155
BseXI	GCAGC	2 cut(s)	792, 1552
BsgI	GTGCAG	1 cut(s)	414
Bsh1285I	CGRYCG	1 cut(s)	55
BshFI	GGCC	2 cut(s)	572, 759
BshNI	GGYRCC	1 cut(s)	945
BshVI	ATCGAT	2 cut(s)	482, 1009
BsiEI	CGRYCG	1 cut(s)	55
BsiHKCI	CYCGRG	2 cut(s)	1126, 1182
BsiSI	CCGG	2 cut(s)	943, 1567
BslFI	GGGAC	2 cut(s)	107, 461
BslI	CCNNNNNNNGG	3 cut(s)	458, 580, 1445
BsmAI	GTCTC	2 cut(s)	624, 1031
BsmFI	GGGAC	2 cut(s)	107, 461
BsnI	GGCC	2 cut(s)	572, 759
BsoBI	CYCGRG	2 cut(s)	1126, 1182
Bsp1286I	GDGCHC	2 cut(s)	155, 1292
Bsp13I	TCCGGA	1 cut(s)	1566
BspACI	CCGC	2 cut(s)	55, 789
BspANI	GGCC	2 cut(s)	572, 759
BspCNI	CTCAG	2 cut(s)	751, 1206
BspDI	ATCGAT	2 cut(s)	482, 1009
BspEI	TCCGGA	1 cut(s)	1566
BspHI	TCATGA	1 cut(s)	439
BspLI	GGNNCC	4 cut(s)	82, 123, 863, 947
BspMI	ACCTGC	1 cut(s)	960
BspPI	GGATC	4 cut(s)	543, 1005, 1359, 1481
BspQI	GCTCTTC	1 cut(s)	1228
BspT107I	GGYRCC	1 cut(s)	945
BspTI	CTTAAG	1 cut(s)	1393
BsrDI	GCAATG	2 cut(s)	37, 1000
BsrFI	RCCGGY	1 cut(s)	942
BsrI	ACTGG	3 cut(s)	84, 328, 612
BssAI	RCCGGY	1 cut(s)	942
Bst2UI	CCWGG	1 cut(s)	1434
Bst4CI	ACNGT	4 cut(s)	170, 338, 933, 1239
Bst6I	CTCTTC	4 cut(s)	627, 1228, 1237, 1483
BstAFI	CTTAAG	1 cut(s)	1393
BstAPI	GCANNNNNTGC	1 cut(s)	952
BstC8I	GCNNGC	2 cut(s)	761, 1179
BstDEI	CTNAG	3 cut(s)	738, 785, 1193
BstENI	CCTNNNNNAGG	1 cut(s)	456
BstF5I	GGATG	3 cut(s)	526, 1492, 1518
BstMAI	GTCTC	2 cut(s)	624, 1031
BstMCI	CGRYCG	1 cut(s)	55
BstMWI	GCNNNNNNNGC	2 cut(s)	324, 952
BstNI	CCWGG	1 cut(s)	1434
BstNSI	RCATGY	2 cut(s)	1177, 1181
BstSCI	CCNGG	1 cut(s)	1432
BstSLI	GKGCMC	1 cut(s)	155
BstV1I	GCAGC	2 cut(s)	792, 1552
BstV2I	GAAGAC	1 cut(s)	580
BstX2I	RGATCY	2 cut(s)	223, 1473
BstYI	RGATCY	2 cut(s)	223, 1473
Bsu15I	ATCGAT	2 cut(s)	482, 1009
BsuRI	GGCC	2 cut(s)	572, 759
BsuTUI	ATCGAT	2 cut(s)	482, 1009
BtrI	CACGTC	1 cut(s)	7
BtsCI	GGATG	3 cut(s)	526, 1492, 1518
BtsIMutI	CAGTG	2 cut(s)	91, 177
BveI	ACCTGC	1 cut(s)	960
Cac8I	GCNNGC	2 cut(s)	761, 1179
CaiI	CAGNNNCTG	1 cut(s)	1445
CciI	TCATGA	1 cut(s)	439
Cfr10I	RCCGGY	1 cut(s)	942
Cfr13I	GGNCC	6 cut(s)	121, 571, 757, 862, 940, 1436
ClaI	ATCGAT	2 cut(s)	482, 1009
CsiI	ACCWGGT	1 cut(s)	1432
Csp6I	GTAC	4 cut(s)	680, 693, 929, 1253
CviAII	CATG	8 cut(s)	246, 261, 391, 440, 1033, 1118, 1174, 1178
CviQI	GTAC	4 cut(s)	680, 693, 929, 1253
DdeI	CTNAG	3 cut(s)	738, 785, 1193
DraI	TTTAAA	1 cut(s)	1146
Eam1104I	CTCTTC	4 cut(s)	627, 1228, 1237, 1483
EarI	CTCTTC	4 cut(s)	627, 1228, 1237, 1483
Eco24I	GRGCYC	1 cut(s)	1292
Eco47I	GGWCC	4 cut(s)	121, 862, 940, 1436
Eco57I	CTGAAG	3 cut(s)	753, 893, 1209
Eco88I	CYCGRG	2 cut(s)	1126, 1182
EcoNI	CCTNNNNNAGG	1 cut(s)	456
EcoRI	GAATTC	1 cut(s)	1066
EcoRII	CCWGG	1 cut(s)	1432
EcoT22I	ATGCAT	1 cut(s)	1179
EcoT38I	GRGCYC	1 cut(s)	1292
FaeI	CATG	8 cut(s)	249, 264, 394, 443, 1036, 1121, 1177, 1181
FalI	AAGNNNNNCTT	6 cut(s)	1207, 1239, 1376, 1408, 1462, 1494
FaqI	GGGAC	2 cut(s)	107, 461
FatI	CATG	8 cut(s)	245, 260, 390, 439, 1032, 1117, 1173, 1177
Fnu4HI	GCNGC	2 cut(s)	806, 1541
FokI	GGATG	3 cut(s)	533, 1479, 1505
FriOI	GRGCYC	1 cut(s)	1292
Fsp4HI	GCNGC	2 cut(s)	806, 1541
FspBI	CTAG	4 cut(s)	69, 99, 354, 1256
GluI	GCNGC	2 cut(s)	806, 1541
HaeIII	GGCC	2 cut(s)	572, 759
HapII	CCGG	2 cut(s)	943, 1567
Hin1II	CATG	8 cut(s)	249, 264, 394, 443, 1036, 1121, 1177, 1181
HincII	GTYRAC	1 cut(s)	910
HindII	GTYRAC	1 cut(s)	910
HinfI	GANTC	4 cut(s)	474, 659, 792, 842
HpaII	CCGG	2 cut(s)	943, 1567
HphI	GGTGA	2 cut(s)	494, 1359
Hpy166II	GTNNAC	1 cut(s)	910
Hpy8I	GTNNAC	1 cut(s)	910
HpyAV	CCTTC	5 cut(s)	774, 919, 1243, 1302, 1392
HpyCH4III	ACNGT	4 cut(s)	170, 338, 933, 1239
HpyCH4IV	ACGT	1 cut(s)	6
HpyF10VI	GCNNNNNNNGC	2 cut(s)	324, 952
HpyF3I	CTNAG	3 cut(s)	738, 785, 1193
HpySE526I	ACGT	1 cut(s)	6
Hsp92II	CATG	8 cut(s)	249, 264, 394, 443, 1036, 1121, 1177, 1181
Kpn2I	TCCGGA	1 cut(s)	1566
LguI	GCTCTTC	1 cut(s)	1228
LmnI	GCTCC	1 cut(s)	461
Lsp1109I	GCAGC	2 cut(s)	792, 1552
LweI	GCATC	1 cut(s)	433
MabI	ACCWGGT	1 cut(s)	1432
MaeI	CTAG	4 cut(s)	69, 99, 354, 1256
MaeII	ACGT	1 cut(s)	6
MaeIII	GTNAC	1 cut(s)	398
MfeI	CAATTG	2 cut(s)	687, 866
MflI	RGATCY	2 cut(s)	223, 1473
MhlI	GDGCHC	2 cut(s)	155, 1292
MlyI	GAGTC	1 cut(s)	483
MmeI	TCCRAC	2 cut(s)	436, 1263
Mph1103I	ATGCAT	1 cut(s)	1179
MroI	TCCGGA	1 cut(s)	1566
MroXI	GAANNNNTTC	1 cut(s)	881
MseI	TTAA	5 cut(s)	564, 1145, 1320, 1394, 1422
MslI	CAYNNNNRTG	2 cut(s)	259, 1116
MspCI	CTTAAG	1 cut(s)	1393
MspI	CCGG	2 cut(s)	943, 1567
MspR9I	CCNGG	1 cut(s)	1434
MunI	CAATTG	2 cut(s)	687, 866
MvaI	CCWGG	1 cut(s)	1434
MwoI	GCNNNNNNNGC	2 cut(s)	324, 952
NlaIII	CATG	8 cut(s)	249, 264, 394, 443, 1036, 1121, 1177, 1181
NlaIV	GGNNCC	4 cut(s)	82, 123, 863, 947
NsiI	ATGCAT	1 cut(s)	1179
NspI	RCATGY	2 cut(s)	1177, 1181
PaeI	GCATGC	1 cut(s)	1181
PaeR7I	CTCGAG	2 cut(s)	1126, 1182
PagI	TCATGA	1 cut(s)	439
PaqCI	CACCTGC	1 cut(s)	960
PciSI	GCTCTTC	1 cut(s)	1228
PdmI	GAANNNNTTC	1 cut(s)	881
PfeI	GAWTC	3 cut(s)	659, 792, 842
PflMI	CCANNNNNTGG	2 cut(s)	580, 1445
PkrI	GCNGC	2 cut(s)	807, 1542
PleI	GAGTC	1 cut(s)	482
PpsI	GAGTC	1 cut(s)	482
PshBI	ATTAAT	1 cut(s)	564
Psp6I	CCWGG	1 cut(s)	1432
PspGI	CCWGG	1 cut(s)	1432
PspN4I	GGNNCC	4 cut(s)	82, 123, 863, 947
PspPI	GGNCC	6 cut(s)	121, 571, 757, 862, 940, 1436
PstNI	CAGNNNCTG	1 cut(s)	1445
PsuI	RGATCY	2 cut(s)	223, 1473
RsaI	GTAC	4 cut(s)	681, 694, 930, 1254
RsaNI	GTAC	4 cut(s)	680, 693, 929, 1253
RseI	CAYNNNNRTG	2 cut(s)	259, 1116
SapI	GCTCTTC	1 cut(s)	1228
SaqAI	TTAA	5 cut(s)	564, 1145, 1320, 1394, 1422
SatI	GCNGC	2 cut(s)	806, 1541
Sau96I	GGNCC	6 cut(s)	121, 571, 757, 862, 940, 1436
SchI	GAGTC	1 cut(s)	483
ScrFI	CCNGG	1 cut(s)	1434
SduI	GDGCHC	2 cut(s)	155, 1292
SexAI	ACCWGGT	1 cut(s)	1432
SfaNI	GCATC	1 cut(s)	433
Sfr274I	CTCGAG	2 cut(s)	1126, 1182
SinI	GGWCC	4 cut(s)	121, 862, 940, 1436
SlaI	CTCGAG	2 cut(s)	1126, 1182
SmiI	ATTTAAAT	1 cut(s)	1146
SmiMI	CAYNNNNRTG	2 cut(s)	259, 1116
SmlI	CTYRAG	4 cut(s)	508, 1126, 1182, 1393
SmoI	CTYRAG	4 cut(s)	508, 1126, 1182, 1393
SphI	GCATGC	1 cut(s)	1181
SsiI	CCGC	2 cut(s)	55, 789
SspI	AATATT	2 cut(s)	414, 1558
SspMI	CTAG	4 cut(s)	69, 99, 354, 1256
StyD4I	CCNGG	1 cut(s)	1432
SwaI	ATTTAAAT	1 cut(s)	1146
TaaI	ACNGT	4 cut(s)	170, 338, 933, 1239
TaiI	ACGT	1 cut(s)	9
TaqI	TCGA	6 cut(s)	51, 482, 857, 1009, 1127, 1183
TfiI	GAWTC	3 cut(s)	659, 792, 842
Tru1I	TTAA	5 cut(s)	564, 1145, 1320, 1394, 1422
Tru9I	TTAA	5 cut(s)	564, 1145, 1320, 1394, 1422
TscAI	CASTG	2 cut(s)	91, 184
TseI	GCWGC	2 cut(s)	805, 1540
TspRI	CASTG	2 cut(s)	91, 184
Van91I	CCANNNNNTGG	2 cut(s)	580, 1445
Vha464I	CTTAAG	1 cut(s)	1393
VpaK11BI	GGWCC	4 cut(s)	121, 862, 940, 1436
VspI	ATTAAT	1 cut(s)	564
XagI	CCTNNNNNAGG	1 cut(s)	456
XapI	RAATTY	7 cut(s)	60, 173, 435, 1066, 1132, 1147, 1418
XceI	RCATGY	2 cut(s)	1177, 1181
XhoI	CTCGAG	2 cut(s)	1126, 1182
XmnI	GAANNNNTTC	1 cut(s)	881
XspI	CTAG	4 cut(s)	69, 99, 354, 1256
Zsp2I	ATGCAT	1 cut(s)	1179

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.