MD02G1296300.v1.1

Plant lipid transfer protein / seed storage protein / trypsin-alpha amylase inhibitor domain family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011865
Physical Location & Seq
Forward (+)
35131188 .. 35132153
966 bp
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UTR
Exon/CDS
Intron
MD02G1296300.v1.1.491

Sequence Viewer

Length: 348 bp
ATGCTCAATTTTCACTTCGCGGGTTTGGCCGTCCTTTTCATCACCGGAGTTGTATTCTCCGGTGAGATTGGCGGGGTTGTTGGGCAGTTATGCGGAGCTGACATTGGAGGACTTGTAACACAATGTGCAGCCTATGTGCAAAAGGGCACGCCCATGACGGATCCATCCGAGGCATGCTGCGAGTTGATTAGGCAAGTGGACATTCCATGCGCATGTAAGCACGTGACCAAGGAGGTGGAACAGATGGTTGACATGAATAAGGTTGTTCATGTCGTTTCCTACTGCGGTAAACCTGTTCCCAAGGGAATGAAGTGTGGAAGTTATACAGTTCCTCCTGTTAGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

116

Amino Acids

12.06

Weight (kDa)

6.24

Isoelectric Point (pI)

19.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 28 - 105 2.1e-13 Probable lipid transfer
Tryp_alpha_amyl PF00234 31 - 105 1.5e-10 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011865)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33550 AT4G33550
fragaria_vesca FvH4_3g29300 FvH4_7g02210
malus_domestica MD02G1296300.v1.1 MD02G1296400.v1.1
prunus_persica Prupe.2G028300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0218541
rosa_laevigata RLG00000002359
rosa_multiflora Rmu_sc0001301.1_g000031
rosa_roxburghii Rroxscaffold_3G00240850
rosa_rugosa Rorug01G0028000 Rorug01G0215600 Rorug07G0178300
rosa_samantha Rh7BG311200 Rh7CG336300 Rh7DG318500
rosa_wichuraiana Rw1G003290 Rw7G027210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 211
AccII CGCG 1 cut(s) 20
AciI CCGC 4 cut(s) 20, 72, 93, 285
AclWI GGATC 2 cut(s) 155, 168
AcoI YGGCCR 1 cut(s) 27
AcvI CACGTG 1 cut(s) 223
AdeI CACNNNGTG 1 cut(s) 125
AfiI CCNNNNNNNGG 1 cut(s) 341
AluBI AGCT 1 cut(s) 98
AluI AGCT 1 cut(s) 98
AlwI GGATC 2 cut(s) 155, 168
AoxI GGCC 1 cut(s) 27
ApeKI GCWGC 2 cut(s) 128, 177
AspLEI GCGC 1 cut(s) 212
AsuHPI GGTGA 2 cut(s) 34, 74
BaeGI GKGCMC 1 cut(s) 149
BamHI GGATCC 1 cut(s) 160
BbrPI CACGTG 1 cut(s) 223
BbvI GCAGC 2 cut(s) 140, 164
BccI CCATC 2 cut(s) 172, 238
BceAI ACGGC 1 cut(s) 14
BisI GCNGC 2 cut(s) 129, 178
BlsI GCNGC 2 cut(s) 130, 179
BmiI GGNNCC 1 cut(s) 162
BsaAI YACGTR 1 cut(s) 223
BsaJI CCNNGG 3 cut(s) 168, 228, 300
BsaWI WCCGGW 2 cut(s) 44, 59
BsaXI ACNNNNNCTCC 4 cut(s) 99, 129, 316, 346
Bsc4I CCNNNNNNNGG 1 cut(s) 341
BseDI CCNNGG 3 cut(s) 168, 228, 300
BseGI GGATG 1 cut(s) 164
BseLI CCNNNNNNNGG 1 cut(s) 341
BseSI GKGCMC 1 cut(s) 149
BseXI GCAGC 2 cut(s) 140, 164
BsgI GTGCAG 1 cut(s) 147
Bsh1236I CGCG 1 cut(s) 20
BshFI GGCC 1 cut(s) 29
BsiSI CCGG 2 cut(s) 45, 60
BslI CCNNNNNNNGG 1 cut(s) 341
BsnI GGCC 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 149
Bsp143I GATC 1 cut(s) 160
BspACI CCGC 4 cut(s) 20, 72, 93, 285
BspANI GGCC 1 cut(s) 29
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 1 cut(s) 162
BspPI GGATC 2 cut(s) 155, 168
BssECI CCNNGG 3 cut(s) 168, 228, 300
BssMI GATC 1 cut(s) 160
BssT1I CCWWGG 2 cut(s) 228, 300
Bst4CI ACNGT 1 cut(s) 328
BstBAI YACGTR 1 cut(s) 223
BstC8I GCNNGC 2 cut(s) 149, 175
BstF5I GGATG 1 cut(s) 164
BstFNI CGCG 1 cut(s) 20
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 1 cut(s) 163
BstMBI GATC 1 cut(s) 160
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstNSI RCATGY 2 cut(s) 177, 216
BstSLI GKGCMC 1 cut(s) 149
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 2 cut(s) 140, 164
BstX2I RGATCY 1 cut(s) 160
BstXI CCANNNNNNTGG 1 cut(s) 235
BstYI RGATCY 1 cut(s) 160
BsuRI GGCC 1 cut(s) 29
BtsCI GGATG 1 cut(s) 164
Cac8I GCNNGC 2 cut(s) 149, 175
CfoI GCGC 1 cut(s) 212
CviAII CATG 6 cut(s) 154, 174, 207, 213, 253, 269
CviJI RGCY 3 cut(s) 29, 98, 131
CviKI_1 RGCY 3 cut(s) 29, 98, 131
DpnI GATC 1 cut(s) 162
DpnII GATC 1 cut(s) 160
DraIII CACNNNGTG 1 cut(s) 125
EaeI YGGCCR 1 cut(s) 27
Eco130I CCWWGG 2 cut(s) 228, 300
Eco72I CACGTG 1 cut(s) 223
EcoT14I CCWWGG 2 cut(s) 228, 300
ErhI CCWWGG 2 cut(s) 228, 300
FaeI CATG 6 cut(s) 157, 177, 210, 216, 256, 272
FaiI YATR 9 cut(s) 91, 135, 155, 175, 208, 214, 254, 270, 324
FatI CATG 6 cut(s) 153, 173, 206, 212, 252, 268
FauI CCCGC 2 cut(s) 13, 65
Fnu4HI GCNGC 2 cut(s) 129, 178
FokI GGATG 1 cut(s) 151
Fsp4HI GCNGC 2 cut(s) 129, 178
FspAI RTGCGCAY 1 cut(s) 211
FspI TGCGCA 1 cut(s) 211
GlaI GCGC 1 cut(s) 211
GluI GCNGC 2 cut(s) 129, 178
HaeIII GGCC 1 cut(s) 29
HapII CCGG 2 cut(s) 45, 60
HhaI GCGC 1 cut(s) 212
Hin1II CATG 6 cut(s) 157, 177, 210, 216, 256, 272
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HpaII CCGG 2 cut(s) 45, 60
HphI GGTGA 2 cut(s) 34, 74
Hpy166II GTNNAC 3 cut(s) 199, 250, 290
Hpy188I TCNGA 1 cut(s) 169
Hpy8I GTNNAC 3 cut(s) 199, 250, 290
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 2 cut(s) 128, 139
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 6 cut(s) 157, 177, 210, 216, 256, 272
HspAI GCGC 1 cut(s) 210
Kzo9I GATC 1 cut(s) 160
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 3 cut(s) 58, 73, 306
Lsp1109I GCAGC 2 cut(s) 140, 164
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 2 cut(s) 115, 223
MalI GATC 1 cut(s) 162
MboI GATC 1 cut(s) 160
MflI RGATCY 1 cut(s) 160
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 1 cut(s) 7
MnlI CCTC 4 cut(s) 101, 163, 226, 342
MslI CAYNNNNRTG 2 cut(s) 152, 211
MspI CCGG 2 cut(s) 45, 60
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 1 cut(s) 26
NdeII GATC 1 cut(s) 160
NlaIII CATG 6 cut(s) 157, 177, 210, 216, 256, 272
NlaIV GGNNCC 1 cut(s) 162
NmuCI GTSAC 1 cut(s) 223
NsbI TGCGCA 1 cut(s) 211
NspI RCATGY 2 cut(s) 177, 216
PaeI GCATGC 1 cut(s) 177
PkrI GCNGC 2 cut(s) 130, 179
PmaCI CACGTG 1 cut(s) 223
PmlI CACGTG 1 cut(s) 223
Ppu21I YACGTR 1 cut(s) 223
PspCI CACGTG 1 cut(s) 223
PspN4I GGNNCC 1 cut(s) 162
PsuI RGATCY 1 cut(s) 160
RseI CAYNNNNRTG 2 cut(s) 152, 211
SatI GCNGC 2 cut(s) 129, 178
Sau3AI GATC 1 cut(s) 160
SduI GDGCHC 1 cut(s) 149
SetI ASST 5 cut(s) 100, 225, 237, 264, 295
SmiMI CAYNNNNRTG 2 cut(s) 152, 211
SphI GCATGC 1 cut(s) 177
Sse9I AATT 1 cut(s) 7
SsiI CCGC 4 cut(s) 20, 72, 93, 285
StyI CCWWGG 2 cut(s) 228, 300
TaaI ACNGT 1 cut(s) 328
TaiI ACGT 1 cut(s) 225
TasI AATT 1 cut(s) 7
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 2 cut(s) 128, 177
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 4 cut(s) 28, 257, 269, 323
TspGWI ACGGA 1 cut(s) 173
XceI RCATGY 2 cut(s) 177, 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.