MD02G1296400.v1.1

Plant lipid transfer protein / seed storage protein / trypsin-alpha amylase inhibitor domain family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011865
Physical Location & Seq
Forward (+)
35144734 .. 35145096
363 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1296400.v1.1.491

Sequence Viewer

Length: 363 bp
ATGCTTAAACTTCGTAATTTCGTGGTCTTGGCCGTCGTTGCGTTGATCGGGGTTGTACTCTCTGGCGAAATGAAGTTGGTTGTTGGGCAGGAATCATGTGAAGGTGACCTTGTGGACATTATAACGCAATGCGCAGCTTATGTGCAAAAGGGTTCGCCCACGACAGATCCATCTAAAGGATGCTGCGATGTGATAAAGAAGGTGGACATTCACTGTGCTTGCAACCACGTCACCAAAGAGGTGGAGGAGGTGGTTGACATGAACAAGGTGCTTCATGTCGTTTCCTACTGCGGTATAACTCTTCCTAAGGGAACCAAATGTGGAAGTAAAAACCCGAACCTAGAAAAATTAATTACTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

121

Amino Acids

12.87

Weight (kDa)

6.79

Isoelectric Point (pI)

19.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 30 - 107 7.4e-15 Probable lipid transfer
Tryp_alpha_amyl PF00234 37 - 107 1.4e-10 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011865)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33550 AT4G33550
fragaria_vesca FvH4_3g29300 FvH4_7g02210
malus_domestica MD02G1296300.v1.1 MD02G1296400.v1.1
prunus_persica Prupe.2G028300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0218541
rosa_laevigata RLG00000002359
rosa_multiflora Rmu_sc0001301.1_g000031
rosa_roxburghii Rroxscaffold_3G00240850
rosa_rugosa Rorug01G0028000 Rorug01G0215600 Rorug07G0178300
rosa_samantha Rh7BG311200 Rh7CG336300 Rh7DG318500
rosa_wichuraiana Rw1G003290 Rw7G027210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 122
Acc16I TGCGCA 1 cut(s) 133
AciI CCGC 1 cut(s) 291
AclWI GGATC 1 cut(s) 161
AcoI YGGCCR 1 cut(s) 30
AfaI GTAC 1 cut(s) 57
AfiI CCNNNNNNNGG 1 cut(s) 176
AjiI CACGTC 1 cut(s) 229
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
AlwI GGATC 1 cut(s) 161
AoxI GGCC 1 cut(s) 30
ApeKI GCWGC 2 cut(s) 134, 183
AseI ATTAAT 1 cut(s) 350
AspLEI GCGC 1 cut(s) 134
AsuHPI GGTGA 2 cut(s) 116, 223
AxyI CCTNAGG 1 cut(s) 306
BbvI GCAGC 2 cut(s) 146, 170
BccI CCATC 1 cut(s) 178
BceAI ACGGC 1 cut(s) 17
BfaI CTAG 1 cut(s) 341
BisI GCNGC 2 cut(s) 135, 184
BlsI GCNGC 2 cut(s) 136, 185
BmgBI CACGTC 1 cut(s) 229
BmiI GGNNCC 1 cut(s) 313
BmsI GCATC 1 cut(s) 170
Bsc4I CCNNNNNNNGG 1 cut(s) 176
Bse21I CCTNAGG 1 cut(s) 306
Bse3DI GCAATG 1 cut(s) 134
BseGI GGATG 1 cut(s) 185
BseLI CCNNNNNNNGG 1 cut(s) 176
BseMI GCAATG 1 cut(s) 134
BseRI GAGGAG 1 cut(s) 260
BseXI GCAGC 2 cut(s) 146, 170
BshFI GGCC 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 176
BsnI GGCC 1 cut(s) 32
Bsp143I GATC 2 cut(s) 45, 166
BspACI CCGC 1 cut(s) 291
BspANI GGCC 1 cut(s) 32
BspLI GGNNCC 1 cut(s) 313
BspPI GGATC 1 cut(s) 161
BsrDI GCAATG 1 cut(s) 134
BssMI GATC 2 cut(s) 45, 166
Bst4CI ACNGT 1 cut(s) 215
Bst6I CTCTTC 1 cut(s) 306
BstC8I GCNNGC 1 cut(s) 220
BstDEI CTNAG 1 cut(s) 306
BstEII GGTNACC 1 cut(s) 104
BstF5I GGATG 1 cut(s) 185
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 2 cut(s) 48, 169
BstMBI GATC 2 cut(s) 45, 166
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstPI GGTNACC 1 cut(s) 104
BstV1I GCAGC 2 cut(s) 146, 170
BstX2I RGATCY 1 cut(s) 166
BstXI CCANNNNNNTGG 1 cut(s) 241
BstYI RGATCY 1 cut(s) 166
Bsu36I CCTNAGG 1 cut(s) 306
BsuRI GGCC 1 cut(s) 32
BtgZI GCGATG 1 cut(s) 201
BtrI CACGTC 1 cut(s) 229
BtsCI GGATG 1 cut(s) 185
BtsIMutI CAGTG 1 cut(s) 211
Cac8I GCNNGC 1 cut(s) 220
CfoI GCGC 1 cut(s) 134
Csp6I GTAC 1 cut(s) 56
CviAII CATG 3 cut(s) 96, 259, 275
CviJI RGCY 2 cut(s) 32, 137
CviKI_1 RGCY 2 cut(s) 32, 137
CviQI GTAC 1 cut(s) 56
DdeI CTNAG 1 cut(s) 306
DpnI GATC 2 cut(s) 47, 168
DpnII GATC 2 cut(s) 45, 166
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
Eco81I CCTNAGG 1 cut(s) 306
Eco91I GGTNACC 1 cut(s) 104
EcoO65I GGTNACC 1 cut(s) 104
FaeI CATG 3 cut(s) 99, 262, 278
FaiI YATR 6 cut(s) 97, 122, 141, 260, 276, 296
FalI AAGNNNNNCTT 2 cut(s) 93, 125
FatI CATG 3 cut(s) 95, 258, 274
Fnu4HI GCNGC 2 cut(s) 135, 184
FokI GGATG 1 cut(s) 192
Fsp4HI GCNGC 2 cut(s) 135, 184
FspBI CTAG 1 cut(s) 341
FspI TGCGCA 1 cut(s) 133
GlaI GCGC 1 cut(s) 133
GluI GCNGC 2 cut(s) 135, 184
HaeIII GGCC 1 cut(s) 32
HhaI GCGC 1 cut(s) 134
Hin1II CATG 3 cut(s) 99, 262, 278
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HinfI GANTC 1 cut(s) 92
HphI GGTGA 2 cut(s) 116, 223
Hpy166II GTNNAC 3 cut(s) 115, 205, 256
Hpy8I GTNNAC 3 cut(s) 115, 205, 256
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 2 cut(s) 95, 193
HpyCH4III ACNGT 1 cut(s) 215
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 2 cut(s) 145, 222
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 1 cut(s) 306
HpySE526I ACGT 1 cut(s) 228
Hsp92II CATG 3 cut(s) 99, 262, 278
HspAI GCGC 1 cut(s) 132
Kzo9I GATC 2 cut(s) 45, 166
LpnPI CCDG 2 cut(s) 48, 74
Lsp1109I GCAGC 2 cut(s) 146, 170
LweI GCATC 1 cut(s) 170
MaeI CTAG 1 cut(s) 341
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 2 cut(s) 104, 229
MalI GATC 2 cut(s) 47, 168
MboI GATC 2 cut(s) 45, 166
MboII GAAGA 1 cut(s) 293
MflI RGATCY 1 cut(s) 166
MluCI AATT 3 cut(s) 16, 347, 351
MnlI CCTC 3 cut(s) 232, 238, 241
MseI TTAA 3 cut(s) 6, 350, 361
MwoI GCNNNNNNNGC 1 cut(s) 38
NdeII GATC 2 cut(s) 45, 166
NlaIII CATG 3 cut(s) 99, 262, 278
NlaIV GGNNCC 1 cut(s) 313
NmuCI GTSAC 2 cut(s) 104, 229
NsbI TGCGCA 1 cut(s) 133
PfeI GAWTC 1 cut(s) 92
PkrI GCNGC 2 cut(s) 136, 185
PshBI ATTAAT 1 cut(s) 350
PsiI TTATAA 1 cut(s) 122
PspEI GGTNACC 1 cut(s) 104
PspN4I GGNNCC 1 cut(s) 313
PsuI RGATCY 1 cut(s) 166
RsaI GTAC 1 cut(s) 57
RsaNI GTAC 1 cut(s) 56
SaqAI TTAA 3 cut(s) 6, 350, 361
SatI GCNGC 2 cut(s) 135, 184
Sau3AI GATC 2 cut(s) 45, 166
SetI ASST 9 cut(s) 106, 111, 139, 204, 231, 243, 252, 270, 342
SfaNI GCATC 1 cut(s) 170
Sse9I AATT 3 cut(s) 16, 347, 351
SsiI CCGC 1 cut(s) 291
SspMI CTAG 1 cut(s) 341
TaaI ACNGT 1 cut(s) 215
TaiI ACGT 1 cut(s) 231
TasI AATT 3 cut(s) 16, 347, 351
TatI WGTACW 1 cut(s) 55
TfiI GAWTC 1 cut(s) 92
Tru1I TTAA 3 cut(s) 6, 350, 361
Tru9I TTAA 3 cut(s) 6, 350, 361
TscAI CASTG 1 cut(s) 218
TseFI GTSAC 2 cut(s) 104, 229
TseI GCWGC 2 cut(s) 134, 183
Tsp45I GTSAC 2 cut(s) 104, 229
TspDTI ATGAA 3 cut(s) 86, 263, 275
TspRI CASTG 1 cut(s) 218
VspI ATTAAT 1 cut(s) 350
XspI CTAG 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.