Rw7G027210
ERF Family

Bifunctional inhibitor lipid-transfer protein seed storage 2S albumin superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011865
Physical Location & Seq
Forward (+)
35055225 .. 35055683
459 bp
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UTR
Exon/CDS
Intron
Rw7G027210.1

Sequence Viewer

Length: 351 bp
ATGCTCAATCTTCCTTTCATGGTTTTGGCTGCCTTTGCGGTGATAGGAATTCTTCTCTCCAGTGAGGTCGATGTGGTTCTGGGGCAGGCTTGCCAAGGTGACATGCAAGGTCTGATAACGCAATGTGCAGCTTATGTTCGGAAGGGTACTCCATTGCCGCCAACGCCGTCTAAAGGATGCTGCACGGCAATCAGAACGTTAGACATTCCATGCGCGTGCCAGCACATGACGAAGATGGTCGAGCAGCTGGTTGACATGGACAAGGTGGTTAAGGTCGTTAGATCCTGTGGTATCGATATTCCTCGTGGAATGAAGTGTGGAAGTTATACAGTTCCACCGTCAAGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

116

Amino Acids

12.29

Weight (kDa)

8.14

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 26 - 106 5.9e-14 Probable lipid transfer
Tryp_alpha_amyl PF00234 31 - 106 3.2e-11 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0011865)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33550 AT4G33550
fragaria_vesca FvH4_3g29300 FvH4_7g02210
malus_domestica MD02G1296300.v1.1 MD02G1296400.v1.1
prunus_persica Prupe.2G028300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0218541
rosa_laevigata RLG00000002359
rosa_multiflora Rmu_sc0001301.1_g000031
rosa_roxburghii Rroxscaffold_3G00240850
rosa_rugosa Rorug01G0028000 Rorug01G0215600 Rorug07G0178300
rosa_samantha Rh7BG311200 Rh7CG336300 Rh7DG318500
rosa_wichuraiana Rw1G003290 Rw7G027210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 215
AciI CCGC 2 cut(s) 38, 158
AclI AACGTT 1 cut(s) 197
AclWI GGATC 1 cut(s) 276
AcsI RAATTY 1 cut(s) 48
AfaI GTAC 1 cut(s) 148
AfiI CCNNNNNNNGG 2 cut(s) 173, 344
AluBI AGCT 2 cut(s) 131, 247
AluI AGCT 2 cut(s) 131, 247
AlwI GGATC 1 cut(s) 276
ApeKI GCWGC 4 cut(s) 29, 128, 180, 244
ApoI RAATTY 1 cut(s) 48
AspLEI GCGC 1 cut(s) 215
AsuHPI GGTGA 2 cut(s) 52, 110
BauI CACGAG 1 cut(s) 303
BbvI GCAGC 4 cut(s) 16, 140, 167, 256
BccI CCATC 1 cut(s) 229
BceAI ACGGC 2 cut(s) 151, 201
BisI GCNGC 5 cut(s) 30, 129, 158, 181, 245
BlsI GCNGC 5 cut(s) 31, 130, 159, 182, 246
BmsI GCATC 1 cut(s) 167
BpmI CTGGAG 1 cut(s) 43
Bsa29I ATCGAT 1 cut(s) 294
BsaJI CCNNGG 1 cut(s) 94
Bsc4I CCNNNNNNNGG 2 cut(s) 173, 344
Bse1I ACTGG 1 cut(s) 60
Bse3DI GCAATG 2 cut(s) 128, 152
BseCI ATCGAT 1 cut(s) 294
BseDI CCNNGG 1 cut(s) 94
BseGI GGATG 1 cut(s) 182
BseLI CCNNNNNNNGG 2 cut(s) 173, 344
BseMI GCAATG 2 cut(s) 128, 152
BseNI ACTGG 1 cut(s) 60
BseXI GCAGC 4 cut(s) 16, 140, 167, 256
BsgI GTGCAG 2 cut(s) 147, 166
Bsh1236I CGCG 1 cut(s) 215
BshVI ATCGAT 1 cut(s) 294
BslI CCNNNNNNNGG 2 cut(s) 173, 344
Bsp143I GATC 1 cut(s) 281
BspACI CCGC 2 cut(s) 38, 158
BspDI ATCGAT 1 cut(s) 294
BspFNI CGCG 1 cut(s) 215
BspPI GGATC 1 cut(s) 276
BsrDI GCAATG 2 cut(s) 128, 152
BsrI ACTGG 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 94
BssMI GATC 1 cut(s) 281
BssSI CACGAG 1 cut(s) 303
BssT1I CCWWGG 1 cut(s) 94
Bst2BI CACGAG 1 cut(s) 303
Bst4CI ACNGT 2 cut(s) 331, 339
BstC8I GCNNGC 4 cut(s) 87, 91, 217, 221
BstF5I GGATG 1 cut(s) 182
BstFNI CGCG 1 cut(s) 215
BstHHI GCGC 1 cut(s) 215
BstKTI GATC 1 cut(s) 284
BstMBI GATC 1 cut(s) 281
BstMWI GCNNNNNNNGC 2 cut(s) 35, 163
BstNSI RCATGY 1 cut(s) 106
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 4 cut(s) 16, 140, 167, 256
BstX2I RGATCY 1 cut(s) 281
BstYI RGATCY 1 cut(s) 281
Bsu15I ATCGAT 1 cut(s) 294
BsuTUI ATCGAT 1 cut(s) 294
BtsCI GGATG 1 cut(s) 182
BtsIMutI CAGTG 1 cut(s) 67
Cac8I GCNNGC 4 cut(s) 87, 91, 217, 221
CfoI GCGC 1 cut(s) 215
ClaI ATCGAT 1 cut(s) 294
Csp6I GTAC 1 cut(s) 147
CviAII CATG 5 cut(s) 19, 103, 210, 226, 256
CviJI RGCY 4 cut(s) 29, 89, 131, 247
CviKI_1 RGCY 4 cut(s) 29, 89, 131, 247
CviQI GTAC 1 cut(s) 147
DpnI GATC 1 cut(s) 283
DpnII GATC 1 cut(s) 281
Eco130I CCWWGG 1 cut(s) 94
EcoRI GAATTC 1 cut(s) 48
EcoT14I CCWWGG 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 94
FaeI CATG 5 cut(s) 22, 106, 213, 229, 259
FaiI YATR 7 cut(s) 20, 104, 135, 211, 227, 257, 327
FatI CATG 5 cut(s) 18, 102, 209, 225, 255
Fnu4HI GCNGC 5 cut(s) 30, 129, 158, 181, 245
FokI GGATG 1 cut(s) 189
Fsp4HI GCNGC 5 cut(s) 30, 129, 158, 181, 245
GlaI GCGC 1 cut(s) 214
GluI GCNGC 5 cut(s) 30, 129, 158, 181, 245
GsuI CTGGAG 1 cut(s) 43
HhaI GCGC 1 cut(s) 215
Hin1II CATG 5 cut(s) 22, 106, 213, 229, 259
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HphI GGTGA 2 cut(s) 52, 110
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 3 cut(s) 114, 141, 194
Hpy8I GTNNAC 1 cut(s) 253
HpyAV CCTTC 1 cut(s) 136
HpyCH4III ACNGT 2 cut(s) 331, 339
HpyCH4IV ACGT 1 cut(s) 197
HpyCH4V TGCA 3 cut(s) 106, 128, 183
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 163
HpySE526I ACGT 1 cut(s) 197
Hsp92II CATG 5 cut(s) 22, 106, 213, 229, 259
HspAI GCGC 1 cut(s) 213
Kzo9I GATC 1 cut(s) 281
LpnPI CCDG 6 cut(s) 65, 71, 73, 233, 233, 298
Lsp1109I GCAGC 4 cut(s) 16, 140, 167, 256
LweI GCATC 1 cut(s) 167
MaeII ACGT 1 cut(s) 197
MaeIII GTNAC 1 cut(s) 98
MalI GATC 1 cut(s) 283
MboI GATC 1 cut(s) 281
MboII GAAGA 2 cut(s) 44, 244
MflI RGATCY 1 cut(s) 281
MluCI AATT 1 cut(s) 48
MnlI CCTC 2 cut(s) 58, 312
MseI TTAA 1 cut(s) 270
MslI CAYNNNNRTG 1 cut(s) 214
MspA1I CMGCKG 1 cut(s) 247
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 2 cut(s) 35, 163
NdeII GATC 1 cut(s) 281
NlaIII CATG 5 cut(s) 22, 106, 213, 229, 259
NmuCI GTSAC 1 cut(s) 98
NspI RCATGY 1 cut(s) 106
PkrI GCNGC 5 cut(s) 31, 130, 159, 182, 246
Psp1406I AACGTT 1 cut(s) 197
PsuI RGATCY 1 cut(s) 281
PvuII CAGCTG 1 cut(s) 247
RsaI GTAC 1 cut(s) 148
RsaNI GTAC 1 cut(s) 147
RseI CAYNNNNRTG 1 cut(s) 214
SaqAI TTAA 1 cut(s) 270
SatI GCNGC 5 cut(s) 30, 129, 158, 181, 245
Sau3AI GATC 1 cut(s) 281
SetI ASST 8 cut(s) 69, 100, 112, 133, 200, 249, 267, 276
SfaNI GCATC 1 cut(s) 167
SmiMI CAYNNNNRTG 1 cut(s) 214
Sse9I AATT 1 cut(s) 48
SsiI CCGC 2 cut(s) 38, 158
StyI CCWWGG 1 cut(s) 94
TaaI ACNGT 2 cut(s) 331, 339
TaiI ACGT 1 cut(s) 200
TaqI TCGA 3 cut(s) 69, 240, 294
TasI AATT 1 cut(s) 48
TauI GCSGC 1 cut(s) 160
Tru1I TTAA 1 cut(s) 270
Tru9I TTAA 1 cut(s) 270
TscAI CASTG 1 cut(s) 67
TseFI GTSAC 1 cut(s) 98
TseI GCWGC 4 cut(s) 29, 128, 180, 244
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 2 cut(s) 7, 326
TspRI CASTG 1 cut(s) 67
XapI RAATTY 1 cut(s) 48
XceI RCATGY 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.