MD02G1306200.v1.1

Polyamine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010313
Physical Location & Seq
Reverse (-)
35927595 .. 35934510
6916 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1306200.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGATTCCCCTTCTAGCTCCTCCGTCATCATTGTCGGCGCCGGAGTCTCCGGTTTATCGGCGGCGAAGGTATTGATCGAGAACGGCGTGGAGGACGTGGTGATTCTGGAAGCTTCCGACCGTATAGGAGGCAGGATCCGAAAGCAGGATTTCGGCGGTGTATCGGTGGAGCTTGGCGCCGGTTGGATAGTCGGAGTCGGAGGCAGAGAGTTGAACCCGGTCTTGGACCTCGCCCTGAAATCGAATCTCCGCACTATCTTCTCCGATTACAGTAATGCTCGCTACAACATCTACGATCGCAGCGGGAAGATATTTCCTAGAGGACTCGTGGAAGAGACGTACAAGAAGGAAGTGGAGTCGGCGGTGCAGAAGCTGAAGAAGCTGGAAGCCGGCGGCGGCGATTTCTCAAATGTCACCGAACCGCCCACTACACAGAAAACGCCGATAGAGCTCGCCATTGATTTCACCCTACACGATTTTGAGATGCCAGAAGTGGAGCCCATATCAACCTTTTTGGATTATGGAGAACGAGAGTTTTTGGTTGCGGATGAGAGAGGGTATGAGCATATGCTGTACAAAATGGCCGAAGATGTTCTCTTTACATCTGAGGGTAAACTGTTGGACAGTCGTCTCAAGTTCAATAAGGTTGTTCGGGAGTTGCAGCACTCAAGAAACGGCGTTACGGTAATGACGGAGGATGGTTGCGTCTTCCAAGCAAATTACATGATTTTGTCAGTTAGCATTGGTGTTCTCCAAAGCAACCTCATTGCTTTCAATCCACCCTTACCCGGTTTGTGGAAAACAGAGGCTATACAGAAATGTGATGTGATTGTATACACGAAGATCTTTCTGAAGTTCCCTTATAAGTTCTGGCCTTGTGGACCAGGACAAGAGTTCTTCCTCTATGCCCATGAGAGGAGAGGCTACTACACATTTTGGCAGCACATGGAAAATGCTTATCCTGGATCCAATATGCTGGTCGTGACGTTGACGAATGGAATCAAGGAGAAGCCTCCAAACCTTCAACTCGTGCACAGTAATCCATATTCAACTTGTTTTTCGATGAGTTTCCACGTCATTCGGTAA

Protein Analysis

362

Amino Acids

40.48

Weight (kDa)

5.62

Isoelectric Point (pI)

37.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 9 - 45 9e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 11 - 72 1e-11 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 16 - 123 2.7e-18 Flavin containing amine oxidoreductase
Amino_oxidase PF01593 173 - 330 5.8e-20 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 864
AccB1I GGYRCC 2 cut(s) 38, 176
AccI GTMKAC 1 cut(s) 834
AciI CCGC 9 cut(s) 62, 156, 250, 303, 362, 393, 396, 422, 545
AclWI GGATC 4 cut(s) 130, 143, 960, 973
AcoI YGGCCR 1 cut(s) 582
AcuI CTGAAG 2 cut(s) 395, 872
AcyI GRCGYC 2 cut(s) 39, 177
AfaI GTAC 2 cut(s) 341, 575
AfiI CCNNNNNNNGG 5 cut(s) 145, 223, 788, 795, 915
AgsI TTSAA 5 cut(s) 214, 640, 775, 1025, 1050
AjiI CACGTC 2 cut(s) 97, 1075
AjnI CCWGG 2 cut(s) 883, 961
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
AluBI AGCT 6 cut(s) 18, 113, 172, 373, 382, 451
AluI AGCT 6 cut(s) 18, 113, 172, 373, 382, 451
Alw21I GWGCWC 2 cut(s) 453, 1035
Alw26I GTCTC 3 cut(s) 52, 329, 635
Alw44I GTGCAC 1 cut(s) 1031
AlwI GGATC 4 cut(s) 130, 143, 960, 973
AlwNI CAGNNNCTG 1 cut(s) 373
AoxI GGCC 2 cut(s) 582, 872
ApaLI GTGCAC 1 cut(s) 1031
ApeKI GCWGC 3 cut(s) 300, 661, 940
Asp700I GAANNNNTTC 1 cut(s) 591
AspLEI GCGC 2 cut(s) 41, 179
AspS9I GGNCC 2 cut(s) 226, 881
AsuC2I CCSGG 2 cut(s) 218, 789
AsuHPI GGTGA 3 cut(s) 112, 406, 457
AvaII GGWCC 2 cut(s) 226, 881
BaeGI GKGCMC 1 cut(s) 1035
BamHI GGATCC 2 cut(s) 135, 965
BanI GGYRCC 2 cut(s) 38, 176
BanII GRGCYC 2 cut(s) 453, 501
BauI CACGAG 2 cut(s) 326, 1028
BbsI GAAGAC 1 cut(s) 700
Bbv12I GWGCWC 2 cut(s) 453, 1035
BbvI GCAGC 3 cut(s) 312, 673, 952
BccI CCATC 1 cut(s) 692
BceAI ACGGC 2 cut(s) 100, 691
BciT130I CCWGG 2 cut(s) 885, 963
BcnI CCSGG 2 cut(s) 218, 789
BcoDI GTCTC 3 cut(s) 52, 329, 635
BfaI CTAG 2 cut(s) 15, 318
BfoI RGCGCY 2 cut(s) 42, 180
BglII AGATCT 1 cut(s) 843
BisI GCNGC 6 cut(s) 63, 301, 394, 397, 662, 941
BlsI GCNGC 6 cut(s) 64, 302, 395, 398, 663, 942
Bme1390I CCNGG 4 cut(s) 218, 789, 885, 963
Bme18I GGWCC 2 cut(s) 226, 881
BmgBI CACGTC 2 cut(s) 97, 1075
BmgT120I GGNCC 2 cut(s) 226, 881
BmiI GGNNCC 5 cut(s) 40, 137, 178, 498, 967
BmrFI CCNGG 4 cut(s) 218, 789, 885, 963
BmsI GCATC 1 cut(s) 474
BoxI GACNNNNGTC 1 cut(s) 627
BpiI GAAGAC 1 cut(s) 700
BpuEI CTTGAG 2 cut(s) 617, 652
BpuMI CCSGG 2 cut(s) 218, 789
BsaHI GRCGYC 2 cut(s) 39, 177
BsaWI WCCGGW 1 cut(s) 50
Bsc4I CCNNNNNNNGG 5 cut(s) 145, 223, 788, 795, 915
Bse118I RCCGGY 2 cut(s) 179, 389
Bse3DI GCAATG 1 cut(s) 765
BseBI CCWGG 2 cut(s) 885, 963
BseGI GGATG 2 cut(s) 553, 703
BseLI CCNNNNNNNGG 5 cut(s) 145, 223, 788, 795, 915
BseMI GCAATG 1 cut(s) 765
BseMII CTCAG 1 cut(s) 597
BseRI GAGGAG 2 cut(s) 10, 931
BseSI GKGCMC 1 cut(s) 1035
BseXI GCAGC 3 cut(s) 312, 673, 952
BsgI GTGCAG 1 cut(s) 386
Bsh1285I CGRYCG 2 cut(s) 121, 298
BshFI GGCC 2 cut(s) 584, 874
BshNI GGYRCC 2 cut(s) 38, 176
BsiEI CGRYCG 2 cut(s) 121, 298
BsiHKAI GWGCWC 2 cut(s) 453, 1035
BsiSI CCGG 6 cut(s) 42, 51, 180, 218, 390, 789
BslI CCNNNNNNNGG 5 cut(s) 145, 223, 788, 795, 915
BsmAI GTCTC 3 cut(s) 52, 329, 635
BsmBI CGTCTC 2 cut(s) 329, 635
BsnI GGCC 2 cut(s) 584, 874
Bsp1286I GDGCHC 3 cut(s) 453, 501, 1035
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 5 cut(s) 75, 135, 295, 843, 965
BspACI CCGC 9 cut(s) 62, 156, 250, 303, 362, 393, 396, 422, 545
BspANI GGCC 2 cut(s) 584, 874
BspCNI CTCAG 1 cut(s) 598
BspLI GGNNCC 5 cut(s) 40, 137, 178, 498, 967
BspPI GGATC 4 cut(s) 130, 143, 960, 973
BspT107I GGYRCC 2 cut(s) 38, 176
BsrDI GCAATG 1 cut(s) 765
BsrFI RCCGGY 2 cut(s) 179, 389
BsrGI TGTACA 1 cut(s) 573
BssAI RCCGGY 2 cut(s) 179, 389
BssMI GATC 5 cut(s) 75, 135, 295, 843, 965
BssNAI GTATAC 1 cut(s) 835
BssNI GRCGYC 2 cut(s) 39, 177
BssSI CACGAG 2 cut(s) 326, 1028
Bst1107I GTATAC 1 cut(s) 835
Bst2BI CACGAG 2 cut(s) 326, 1028
Bst2UI CCWGG 2 cut(s) 885, 963
Bst4CI ACNGT 6 cut(s) 122, 272, 618, 626, 685, 1037
Bst6I CTCTTC 1 cut(s) 327
BstACI GRCGYC 2 cut(s) 39, 177
BstAUI TGTACA 1 cut(s) 573
BstC8I GCNNGC 3 cut(s) 280, 391, 453
BstDEI CTNAG 1 cut(s) 606
BstF5I GGATG 2 cut(s) 553, 703
BstH2I RGCGCY 2 cut(s) 42, 180
BstHHI GCGC 2 cut(s) 41, 179
BstKTI GATC 5 cut(s) 78, 138, 298, 846, 968
BstMAI GTCTC 3 cut(s) 52, 329, 635
BstMBI GATC 5 cut(s) 75, 135, 295, 843, 965
BstMCI CGRYCG 2 cut(s) 121, 298
BstMWI GCNNNNNNNGC 2 cut(s) 379, 448
BstNI CCWGG 2 cut(s) 885, 963
BstPAI GACNNNNGTC 1 cut(s) 627
BstSCI CCNGG 4 cut(s) 216, 787, 883, 961
BstSLI GKGCMC 1 cut(s) 1035
BstV1I GCAGC 3 cut(s) 312, 673, 952
BstV2I GAAGAC 1 cut(s) 700
BstX2I RGATCY 3 cut(s) 135, 843, 965
BstXI CCANNNNNNTGG 1 cut(s) 976
BstYI RGATCY 3 cut(s) 135, 843, 965
BstZ17I GTATAC 1 cut(s) 835
BsuRI GGCC 2 cut(s) 584, 874
BtrI CACGTC 2 cut(s) 97, 1075
BtsCI GGATG 2 cut(s) 553, 703
Cac8I GCNNGC 3 cut(s) 280, 391, 453
CaiI CAGNNNCTG 1 cut(s) 373
CfoI GCGC 2 cut(s) 41, 179
Cfr10I RCCGGY 2 cut(s) 179, 389
Cfr13I GGNCC 2 cut(s) 226, 881
CseI GACGC 1 cut(s) 694
Csp6I GTAC 2 cut(s) 340, 574
CviAII CATG 3 cut(s) 724, 911, 946
CviQI GTAC 2 cut(s) 340, 574
DdeI CTNAG 1 cut(s) 606
DinI GGCGCC 2 cut(s) 40, 178
DpnI GATC 5 cut(s) 77, 137, 297, 845, 967
DpnII GATC 5 cut(s) 75, 135, 295, 843, 965
EaeI YGGCCR 1 cut(s) 582
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Ecl136II GAGCTC 1 cut(s) 451
Eco24I GRGCYC 2 cut(s) 453, 501
Eco47I GGWCC 2 cut(s) 226, 881
Eco53kI GAGCTC 1 cut(s) 451
Eco57I CTGAAG 2 cut(s) 395, 872
EcoICRI GAGCTC 1 cut(s) 451
EcoRII CCWGG 2 cut(s) 883, 961
EcoT38I GRGCYC 2 cut(s) 453, 501
EgeI GGCGCC 2 cut(s) 40, 178
EheI GGCGCC 2 cut(s) 40, 178
Esp3I CGTCTC 2 cut(s) 329, 635
FaeI CATG 3 cut(s) 727, 914, 949
FatI CATG 3 cut(s) 723, 910, 945
FauI CCCGC 1 cut(s) 296
FauNDI CATATG 1 cut(s) 567
FblI GTMKAC 1 cut(s) 834
Fnu4HI GCNGC 6 cut(s) 63, 301, 394, 397, 662, 941
FokI GGATG 2 cut(s) 560, 710
FriOI GRGCYC 2 cut(s) 453, 501
Fsp4HI GCNGC 6 cut(s) 63, 301, 394, 397, 662, 941
FspBI CTAG 2 cut(s) 15, 318
GlaI GCGC 2 cut(s) 40, 178
GluI GCNGC 6 cut(s) 63, 301, 394, 397, 662, 941
HaeII RGCGCY 2 cut(s) 42, 180
HaeIII GGCC 2 cut(s) 584, 874
HapII CCGG 6 cut(s) 42, 51, 180, 218, 390, 789
HgaI GACGC 1 cut(s) 694
HhaI GCGC 2 cut(s) 41, 179
Hin1I GRCGYC 2 cut(s) 39, 177
Hin1II CATG 3 cut(s) 727, 914, 949
Hin6I GCGC 2 cut(s) 39, 177
HinP1I GCGC 2 cut(s) 39, 177
HincII GTYRAC 1 cut(s) 990
HindII GTYRAC 1 cut(s) 990
HindIII AAGCTT 1 cut(s) 111
HinfI GANTC 8 cut(s) 5, 45, 103, 195, 244, 324, 356, 999
HpaII CCGG 6 cut(s) 42, 51, 180, 218, 390, 789
HphI GGTGA 3 cut(s) 112, 406, 457
Hpy166II GTNNAC 5 cut(s) 614, 835, 881, 990, 1033
Hpy188I TCNGA 7 cut(s) 118, 140, 194, 200, 265, 607, 852
Hpy188III TCNNGA 5 cut(s) 79, 107, 653, 669, 982
Hpy8I GTNNAC 5 cut(s) 614, 835, 881, 990, 1033
HpyAV CCTTC 4 cut(s) 21, 61, 340, 1031
HpyCH4III ACNGT 6 cut(s) 122, 272, 618, 626, 685, 1037
HpyCH4IV ACGT 4 cut(s) 96, 338, 986, 1074
HpyCH4V TGCA 3 cut(s) 367, 661, 1033
HpyF10VI GCNNNNNNNGC 2 cut(s) 379, 448
HpyF3I CTNAG 1 cut(s) 606
HpySE526I ACGT 4 cut(s) 96, 338, 986, 1074
Hsp92I GRCGYC 2 cut(s) 39, 177
Hsp92II CATG 3 cut(s) 727, 914, 949
HspAI GCGC 2 cut(s) 39, 177
KasI GGCGCC 2 cut(s) 38, 176
KroI GCCGGC 1 cut(s) 389
KroNI GCCGGC 1 cut(s) 391
Kzo9I GATC 5 cut(s) 75, 135, 295, 843, 965
LmnI GCTCC 3 cut(s) 23, 169, 496
Lsp1109I GCAGC 3 cut(s) 312, 673, 952
LweI GCATC 1 cut(s) 474
MaeI CTAG 2 cut(s) 15, 318
MaeII ACGT 4 cut(s) 96, 338, 986, 1074
MaeIII GTNAC 3 cut(s) 412, 679, 982
MalI GATC 5 cut(s) 77, 137, 297, 845, 967
MboI GATC 5 cut(s) 75, 135, 295, 843, 965
MboII GAAGA 8 cut(s) 250, 319, 344, 388, 599, 700, 853, 889
MflI RGATCY 3 cut(s) 135, 843, 965
MhlI GDGCHC 3 cut(s) 453, 501, 1035
MluCI AATT 1 cut(s) 718
Mly113I GGCGCC 2 cut(s) 39, 177
MlyI GAGTC 4 cut(s) 54, 204, 318, 365
MmeI TCCRAC 5 cut(s) 141, 164, 172, 178, 600
MroNI GCCGGC 1 cut(s) 389
MroXI GAANNNNTTC 1 cut(s) 591
MspA1I CMGCKG 1 cut(s) 303
MspI CCGG 6 cut(s) 42, 51, 180, 218, 390, 789
MspR9I CCNGG 4 cut(s) 218, 789, 885, 963
MvaI CCWGG 2 cut(s) 885, 963
MwoI GCNNNNNNNGC 2 cut(s) 379, 448
NaeI GCCGGC 1 cut(s) 391
NarI GGCGCC 2 cut(s) 39, 177
NciI CCSGG 2 cut(s) 218, 789
NdeI CATATG 1 cut(s) 567
NdeII GATC 5 cut(s) 75, 135, 295, 843, 965
NgoMIV GCCGGC 1 cut(s) 389
NlaIII CATG 3 cut(s) 727, 914, 949
NlaIV GGNNCC 5 cut(s) 40, 137, 178, 498, 967
NmuCI GTSAC 2 cut(s) 412, 982
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PdiI GCCGGC 1 cut(s) 391
PdmI GAANNNNTTC 1 cut(s) 591
PfeI GAWTC 4 cut(s) 5, 103, 244, 999
PfoI TCCNGGA 1 cut(s) 961
PkrI GCNGC 6 cut(s) 64, 302, 395, 398, 663, 942
Ple19I CGATCG 1 cut(s) 298
PleI GAGTC 4 cut(s) 53, 203, 318, 364
PluTI GGCGCC 2 cut(s) 42, 180
PpsI GAGTC 4 cut(s) 53, 203, 318, 364
PshAI GACNNNNGTC 1 cut(s) 627
PsiI TTATAA 1 cut(s) 864
Psp124BI GAGCTC 1 cut(s) 453
Psp6I CCWGG 2 cut(s) 883, 961
PspGI CCWGG 2 cut(s) 883, 961
PspN4I GGNNCC 5 cut(s) 40, 137, 178, 498, 967
PspPI GGNCC 2 cut(s) 226, 881
PstNI CAGNNNCTG 1 cut(s) 373
PsuI RGATCY 3 cut(s) 135, 843, 965
PvuI CGATCG 1 cut(s) 298
RsaI GTAC 2 cut(s) 341, 575
RsaNI GTAC 2 cut(s) 340, 574
SacI GAGCTC 1 cut(s) 453
SatI GCNGC 6 cut(s) 63, 301, 394, 397, 662, 941
Sau3AI GATC 5 cut(s) 75, 135, 295, 843, 965
Sau96I GGNCC 2 cut(s) 226, 881
SchI GAGTC 4 cut(s) 54, 204, 318, 365
ScrFI CCNGG 4 cut(s) 218, 789, 885, 963
SduI GDGCHC 3 cut(s) 453, 501, 1035
SfaNI GCATC 1 cut(s) 474
SfoI GGCGCC 2 cut(s) 40, 178
SinI GGWCC 2 cut(s) 226, 881
SmlI CTYRAG 2 cut(s) 632, 667
SmoI CTYRAG 2 cut(s) 632, 667
Sse9I AATT 1 cut(s) 718
SsiI CCGC 9 cut(s) 62, 156, 250, 303, 362, 393, 396, 422, 545
SspDI GGCGCC 2 cut(s) 38, 176
SspMI CTAG 2 cut(s) 15, 318
SstI GAGCTC 1 cut(s) 453
StyD4I CCNGG 4 cut(s) 216, 787, 883, 961
TaaI ACNGT 6 cut(s) 122, 272, 618, 626, 685, 1037
TaiI ACGT 4 cut(s) 99, 341, 989, 1077
TaqI TCGA 3 cut(s) 78, 242, 1061
TasI AATT 1 cut(s) 718
TatI WGTACW 1 cut(s) 573
TauI GCSGC 3 cut(s) 65, 396, 399
TfiI GAWTC 4 cut(s) 5, 103, 244, 999
TseFI GTSAC 2 cut(s) 412, 982
TseI GCWGC 3 cut(s) 300, 661, 940
Tsp45I GTSAC 2 cut(s) 412, 982
TspGWI ACGGA 2 cut(s) 13, 707
VneI GTGCAC 1 cut(s) 1031
VpaK11BI GGWCC 2 cut(s) 226, 881
XmiI GTMKAC 1 cut(s) 834
XmnI GAANNNNTTC 1 cut(s) 591
XspI CTAG 2 cut(s) 15, 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.