Rorug01G0015600

Polyamine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0010313
Physical Location & Seq
Forward (+)
2747935 .. 2764067
16133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0015600.1

Sequence Viewer

Length: 1797 bp
ATGGCGCAAAAAAAAGGAAACGATGTAGTCTCAGCAAAAGATATGTTCCAACCCTGCAACGTGGAGGAAGAAACACATGATGAGACAATAATCTCAATCTCTATCCCTGAAATAGACGACGAACATGAGGACTGGGTGTTCGAAAGACGAAGGTATATCAAAAGATCTGTGCCTCTATACAATTCGTGTCTGAAAGGTGACTGGAAAGAGGCTAAGAGAATTATAGGCGAAGATCCCAATATTATACGTGCCAGTATCACACACGGGTGCCAGAAACCTCTTCATGTTGCGGCAGGAGCGGGACAAGTTCTTTTTGTGGAGCAGTTGATGAAATATATAACCGAAGAAGATTTACTCTCTAAAGATGCAAAGGGCAATACAGCCTTTTGCTTTGCTGTTGCAGTTGGGTCTGTACCCATCGCTAAGCTCATGATCAGAAAGAACCCACAACTGCCTCAAATTCGCGGTGGGGAAGGAATGACAACGCTTTATATGGCCACTTTGTTTGGACACGAAGAGATGGCGTGGTTTCTGTATCCTAAGCTTATAGAAGATATTGATGTACGGGACCGACTCGGGATATTTTTCAGTTGCATCCACAATGATTTATATGAAATAGCCTTGAAAATGCTTCATGACTACCGAGAGCTTGCTGTGGCTCGTGATATGAATGGTGAAACAGCGGTACATTTGTTGGCACGCAAGCCTTCAATATTTTCCAACAAAAGACCTGGAATATGGAAATGCTTCGTTCAGTCTCTTGTTGGAGGAACAAATAACAAAAAATACTTGGGAACTCCTGGCATTCAATTACTGAAGTTTCTTTGGGAAACCGTTGTCTGGCAACATGACTGGATGGTTCTGGACGTTATAAGAAGGCCATCACATGCTTTATTTATCGCAACAAAAATGGGAAATTTTGAGTTTGTGGCCGAGCTTCTGCACTCGTATCCTGACTTGATATGGGAAACAGATGAAAAGCACCGGAGTGTATTTCACATTGCAGTTATGTTCCATGACACAAGTTTCTTCAATCTGCTAAATCAGTTAGGTGTATACAAGGATTTTATAGTCTCCTTCAAGGACGATGAGAACAATAACATACTGCATTTAGCGGCAAAACTGGCTCCACCAAATCAATTGGGTGCCGTGCCAGGACCTGCCTTTCAAATGCAACGGGAGTTACTGTGGTTCCAGGGGCTGGAAAGGACTCTCCAACGTTCCTATCTAGAGATGAAAAATTCAGAAGGTAAAACACCTAAAGATTTATTTACCGAAGAGCATAAAGGTTTAATGGTAAAGGGAGAATCGTGGATGAAAAGTTTGGCCAGCTCATGCACGCTTGCTTCAACACTTATCGCCATTTCTGTTTTTACTTCACTCACCAGTGTCATTGATGACAGAATCACTTATAATGGAGGAGGAACACATACGACACCGCCAGTTTGTGTCTTATCAAATATTTTTGCATTATTTTACTCTTCGCTTGGAATAATAATATTCGTGTCCATCCTGTCATCACGCTTCGCAGAGGATGATTTCCTCATATCACTGCCCTTGAAGCTCATAGTTGGACTCGGATCACTCTACATCTCTACTATAGCCATGATGGTTTCTTTTGGCACCGCCTTATACACAACATATCACCATAGACTAAACTGGCTTCCTGTTCTTGTTTTCATACTGGCATCCCTGCTACTTAGTTGTCTTTATCGTCTGCACAGTCCTCTCGTTTCCTATGTATTACACACAACGTACCATTCTTGGGTGAGATTACCAACTACACATAACTTGTAA

Protein Analysis

598

Amino Acids

67.94

Weight (kDa)

6.54

Isoelectric Point (pI)

38.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 94 - 190 9.3e-09 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 872, 1413
Acc36I ACCTGC 1 cut(s) 1168
AccB1I GGYRCC 3 cut(s) 267, 1145, 1622
AccB7I CCANNNNNTGG 1 cut(s) 1201
AccBSI CCGCTC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 1056
AccII CGCG 1 cut(s) 465
AciI CCGC 7 cut(s) 290, 299, 465, 683, 1115, 1439, 1626
AclI AACGTT 1 cut(s) 1219
AclWI GGATC 2 cut(s) 227, 1588
AcoI YGGCCR 3 cut(s) 495, 930, 1326
AcsI RAATTY 3 cut(s) 459, 916, 1240
AcuI CTGAAG 1 cut(s) 836
AfaI GTAC 4 cut(s) 414, 564, 687, 1757
AfiI CCNNNNNNNGG 3 cut(s) 840, 1201, 1765
AgsI TTSAA 8 cut(s) 625, 711, 809, 1033, 1081, 1169, 1350, 1561
AjnI CCWGG 4 cut(s) 730, 799, 1153, 1194
AleI CACNNNNGTG 2 cut(s) 265, 987
AloI GAACNNNNNNTCC 2 cut(s) 122, 154
AluBI AGCT 6 cut(s) 427, 544, 649, 937, 1332, 1564
AluI AGCT 6 cut(s) 427, 544, 649, 937, 1332, 1564
Alw26I GTCTC 4 cut(s) 34, 77, 762, 1078
AlwI GGATC 2 cut(s) 227, 1588
AlwNI CAGNNNCTG 2 cut(s) 1160, 1201
Ama87I CYCGRG 1 cut(s) 575
AoxI GGCC 4 cut(s) 495, 878, 930, 1326
ApoI RAATTY 3 cut(s) 459, 916, 1240
ArsI GACNNNNNNTTYG 2 cut(s) 122, 154
Asp700I GAANNNNTTC 1 cut(s) 746
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 568, 1157
AsuHPI GGTGA 5 cut(s) 209, 686, 1375, 1637, 1780
AsuII TTCGAA 1 cut(s) 141
AvaI CYCGRG 1 cut(s) 575
AvaII GGWCC 2 cut(s) 568, 1157
BaeI ACNNNNGTAYC 2 cut(s) 677, 710
BalI TGGCCA 2 cut(s) 497, 1328
BanI GGYRCC 3 cut(s) 267, 1145, 1622
BarI GAAGNNNNNNTAC 2 cut(s) 336, 368
BauI CACGAG 1 cut(s) 660
BccI CCATC 6 cut(s) 425, 514, 850, 889, 1517, 1603
BceAI ACGGC 1 cut(s) 1133
BciT130I CCWGG 4 cut(s) 732, 801, 1155, 1196
BciVI GTATCC 2 cut(s) 546, 960
BclI TGATCA 1 cut(s) 432
BcoDI GTCTC 4 cut(s) 34, 77, 762, 1078
BfaI CTAG 1 cut(s) 1229
BfmI CTRYAG 1 cut(s) 1599
BfuAI ACCTGC 1 cut(s) 1168
BfuI GTATCC 2 cut(s) 546, 960
BglII AGATCT 1 cut(s) 164
BisI GCNGC 2 cut(s) 291, 1116
BlpI GCTNAGC 1 cut(s) 423
BlsI GCNGC 2 cut(s) 292, 1117
Bme1390I CCNGG 4 cut(s) 732, 801, 1155, 1196
Bme18I GGWCC 2 cut(s) 568, 1157
BmeT110I CYCGRG 1 cut(s) 575
BmgT120I GGNCC 2 cut(s) 568, 1157
BmiI GGNNCC 6 cut(s) 269, 569, 1128, 1147, 1193, 1624
BmrFI CCNGG 4 cut(s) 732, 801, 1155, 1196
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 3 cut(s) 355, 603, 1697
BmuI ACTGGG 1 cut(s) 142
Bpu10I CCTNAGC 1 cut(s) 540
Bpu1102I GCTNAGC 1 cut(s) 423
Bpu14I TTCGAA 1 cut(s) 141
BsaAI YACGTR 1 cut(s) 248
BsaJI CCNNGG 1 cut(s) 1195
BsaWI WCCGGW 1 cut(s) 984
BsaXI ACNNNNNCTCC 2 cut(s) 1172, 1202
Bsc4I CCNNNNNNNGG 3 cut(s) 840, 1201, 1765
Bse1I ACTGG 9 cut(s) 137, 206, 252, 857, 1128, 1386, 1442, 1664, 1689
Bse3DI GCAATG 1 cut(s) 999
BseBI CCWGG 4 cut(s) 732, 801, 1155, 1196
BseDI CCNNGG 1 cut(s) 1195
BseGI GGATG 6 cut(s) 594, 861, 1320, 1509, 1540, 1688
BseLI CCNNNNNNNGG 3 cut(s) 840, 1201, 1765
BseMI GCAATG 1 cut(s) 999
BseMII CTCAG 1 cut(s) 45
BseNI ACTGG 9 cut(s) 137, 206, 252, 857, 1128, 1386, 1442, 1664, 1689
BseRI GAGGAG 1 cut(s) 1434
BsgI GTGCAG 2 cut(s) 926, 1703
Bsh1236I CGCG 1 cut(s) 465
BshFI GGCC 4 cut(s) 497, 880, 932, 1328
BshNI GGYRCC 3 cut(s) 267, 1145, 1622
BsiHKCI CYCGRG 1 cut(s) 575
BsiSI CCGG 1 cut(s) 985
BslFI GGGAC 2 cut(s) 315, 581
BslI CCNNNNNNNGG 3 cut(s) 840, 1201, 1765
BsmAI GTCTC 4 cut(s) 34, 77, 762, 1078
BsmFI GGGAC 2 cut(s) 315, 581
BsmI GAATGC 1 cut(s) 804
BsnI GGCC 4 cut(s) 497, 880, 932, 1328
BsoBI CYCGRG 1 cut(s) 575
Bsp119I TTCGAA 1 cut(s) 141
Bsp143I GATC 4 cut(s) 164, 232, 432, 1580
Bsp1720I GCTNAGC 1 cut(s) 423
BspACI CCGC 7 cut(s) 290, 299, 465, 683, 1115, 1439, 1626
BspANI GGCC 4 cut(s) 497, 880, 932, 1328
BspCNI CTCAG 1 cut(s) 44
BspFNI CGCG 1 cut(s) 465
BspHI TCATGA 2 cut(s) 429, 634
BspLI GGNNCC 6 cut(s) 269, 569, 1128, 1147, 1193, 1624
BspMI ACCTGC 1 cut(s) 1168
BspPI GGATC 2 cut(s) 227, 1588
BspQI GCTCTTC 1 cut(s) 1272
BspT104I TTCGAA 1 cut(s) 141
BspT107I GGYRCC 3 cut(s) 267, 1145, 1622
BsrBI CCGCTC 1 cut(s) 299
BsrDI GCAATG 1 cut(s) 999
BsrI ACTGG 9 cut(s) 137, 206, 252, 857, 1128, 1386, 1442, 1664, 1689
BssECI CCNNGG 1 cut(s) 1195
BssMI GATC 4 cut(s) 164, 232, 432, 1580
BssNAI GTATAC 1 cut(s) 1057
BssSI CACGAG 1 cut(s) 660
Bst1107I GTATAC 1 cut(s) 1057
Bst2BI CACGAG 1 cut(s) 660
Bst2UI CCWGG 4 cut(s) 732, 801, 1155, 1196
Bst4CI ACNGT 3 cut(s) 835, 1188, 1724
Bst6I CTCTTC 4 cut(s) 285, 510, 1272, 1486
BstBAI YACGTR 1 cut(s) 248
BstBI TTCGAA 1 cut(s) 141
BstC8I GCNNGC 6 cut(s) 651, 700, 704, 1330, 1340, 1344
BstDEI CTNAG 5 cut(s) 31, 213, 423, 540, 1700
BstF5I GGATG 6 cut(s) 594, 861, 1320, 1509, 1540, 1688
BstFNI CGCG 1 cut(s) 465
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 4 cut(s) 167, 235, 435, 1583
BstMAI GTCTC 4 cut(s) 34, 77, 762, 1078
BstMBI GATC 4 cut(s) 164, 232, 432, 1580
BstMWI GCNNNNNNNGC 3 cut(s) 296, 1124, 1561
BstNI CCWGG 4 cut(s) 732, 801, 1155, 1196
BstNSI RCATGY 1 cut(s) 890
BstSCI CCNGG 4 cut(s) 730, 799, 1153, 1194
BstSFI CTRYAG 1 cut(s) 1599
BstUI CGCG 1 cut(s) 465
BstX2I RGATCY 2 cut(s) 164, 232
BstYI RGATCY 2 cut(s) 164, 232
BstZ17I GTATAC 1 cut(s) 1057
BsuI GTATCC 2 cut(s) 546, 960
BsuRI GGCC 4 cut(s) 497, 880, 932, 1328
BtgZI GCGATG 1 cut(s) 403
BtsCI GGATG 6 cut(s) 594, 861, 1320, 1509, 1540, 1688
BtsI GCAGTG 1 cut(s) 1550
BtsIMutI CAGTG 2 cut(s) 1393, 1550
BveI ACCTGC 1 cut(s) 1168
Cac8I GCNNGC 6 cut(s) 651, 700, 704, 1330, 1340, 1344
CaiI CAGNNNCTG 2 cut(s) 1160, 1201
CciI TCATGA 2 cut(s) 429, 634
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 568, 1157
Csp6I GTAC 4 cut(s) 413, 563, 686, 1756
CspCI CAANNNNNGTGG 2 cut(s) 487, 522
CviQI GTAC 4 cut(s) 413, 563, 686, 1756
DdeI CTNAG 5 cut(s) 31, 213, 423, 540, 1700
DpnI GATC 4 cut(s) 166, 234, 434, 1582
DpnII GATC 4 cut(s) 164, 232, 432, 1580
EaeI YGGCCR 3 cut(s) 495, 930, 1326
Eam1104I CTCTTC 4 cut(s) 285, 510, 1272, 1486
EarI CTCTTC 4 cut(s) 285, 510, 1272, 1486
Eco47I GGWCC 2 cut(s) 568, 1157
Eco57I CTGAAG 1 cut(s) 836
Eco88I CYCGRG 1 cut(s) 575
EcoO109I RGGNCCY 1 cut(s) 1157
EcoRII CCWGG 4 cut(s) 730, 799, 1153, 1194
FaqI GGGAC 2 cut(s) 315, 581
FauI CCCGC 1 cut(s) 292
FbaI TGATCA 1 cut(s) 432
FblI GTMKAC 1 cut(s) 1056
Fnu4HI GCNGC 2 cut(s) 291, 1116
FokI GGATG 6 cut(s) 581, 868, 1327, 1496, 1547, 1675
Fsp4HI GCNGC 2 cut(s) 291, 1116
FspBI CTAG 1 cut(s) 1229
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 291, 1116
HaeIII GGCC 4 cut(s) 497, 880, 932, 1328
HapII CCGG 1 cut(s) 985
HhaI GCGC 1 cut(s) 7
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HindIII AAGCTT 1 cut(s) 542
HinfI GANTC 5 cut(s) 573, 1210, 1307, 1404, 1575
HpaII CCGG 1 cut(s) 985
HphI GGTGA 5 cut(s) 209, 686, 1375, 1637, 1780
Hpy166II GTNNAC 1 cut(s) 1057
Hpy188I TCNGA 4 cut(s) 192, 437, 1246, 1580
Hpy188III TCNNGA 7 cut(s) 430, 577, 635, 662, 863, 953, 1229
Hpy8I GTNNAC 1 cut(s) 1057
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 6 cut(s) 144, 467, 717, 870, 1087, 1241
HpyCH4III ACNGT 3 cut(s) 835, 1188, 1724
HpyCH4IV ACGT 5 cut(s) 60, 247, 867, 1219, 1754
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 1124, 1561
HpyF3I CTNAG 5 cut(s) 31, 213, 423, 540, 1700
HpySE526I ACGT 5 cut(s) 60, 247, 867, 1219, 1754
HspAI GCGC 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 432
Kzo9I GATC 4 cut(s) 164, 232, 432, 1580
LguI GCTCTTC 1 cut(s) 1272
LmnI GCTCC 3 cut(s) 296, 319, 1132
LweI GCATC 3 cut(s) 355, 603, 1697
MaeI CTAG 1 cut(s) 1229
MaeII ACGT 5 cut(s) 60, 247, 867, 1219, 1754
MaeIII GTNAC 2 cut(s) 197, 1182
MalI GATC 4 cut(s) 166, 234, 434, 1582
MbiI CCGCTC 1 cut(s) 299
MboI GATC 4 cut(s) 164, 232, 432, 1580
MfeI CAATTG 1 cut(s) 1139
MflI RGATCY 2 cut(s) 164, 232
MlsI TGGCCA 2 cut(s) 497, 1328
MluCI AATT 7 cut(s) 181, 219, 459, 809, 916, 1139, 1240
MluNI TGGCCA 2 cut(s) 497, 1328
MlyI GAGTC 3 cut(s) 567, 1204, 1569
MmeI TCCRAC 5 cut(s) 73, 744, 745, 1240, 1552
Mox20I TGGCCA 2 cut(s) 497, 1328
MroXI GAANNNNTTC 1 cut(s) 746
MscI TGGCCA 2 cut(s) 497, 1328
MseI TTAA 1 cut(s) 1292
MslI CAYNNNNRTG 2 cut(s) 265, 987
Msp20I TGGCCA 2 cut(s) 497, 1328
MspA1I CMGCKG 1 cut(s) 683
MspI CCGG 1 cut(s) 985
MspR9I CCNGG 4 cut(s) 732, 801, 1155, 1196
MunI CAATTG 1 cut(s) 1139
Mva1269I GAATGC 1 cut(s) 804
MvaI CCWGG 4 cut(s) 732, 801, 1155, 1196
MvnI CGCG 1 cut(s) 465
MwoI GCNNNNNNNGC 3 cut(s) 296, 1124, 1561
NdeII GATC 4 cut(s) 164, 232, 432, 1580
NlaIV GGNNCC 6 cut(s) 269, 569, 1128, 1147, 1193, 1624
NmeAIII GCCGAG 1 cut(s) 958
NmuCI GTSAC 1 cut(s) 197
NspI RCATGY 1 cut(s) 890
NspV TTCGAA 1 cut(s) 141
OliI CACNNNNGTG 2 cut(s) 265, 987
PagI TCATGA 2 cut(s) 429, 634
PciSI GCTCTTC 1 cut(s) 1272
PctI GAATGC 1 cut(s) 804
PdmI GAANNNNTTC 1 cut(s) 746
PfeI GAWTC 2 cut(s) 1307, 1404
PflMI CCANNNNNTGG 1 cut(s) 1201
PkrI GCNGC 2 cut(s) 292, 1117
PleI GAGTC 3 cut(s) 567, 1204, 1569
PpsI GAGTC 3 cut(s) 567, 1204, 1569
Ppu21I YACGTR 1 cut(s) 248
PpuMI RGGWCCY 1 cut(s) 1157
PsiI TTATAA 2 cut(s) 872, 1413
Psp1406I AACGTT 1 cut(s) 1219
Psp5II RGGWCCY 1 cut(s) 1157
Psp6I CCWGG 4 cut(s) 730, 799, 1153, 1194
PspGI CCWGG 4 cut(s) 730, 799, 1153, 1194
PspN4I GGNNCC 6 cut(s) 269, 569, 1128, 1147, 1193, 1624
PspPI GGNCC 2 cut(s) 568, 1157
PspPPI RGGWCCY 1 cut(s) 1157
PstNI CAGNNNCTG 2 cut(s) 1160, 1201
PsuI RGATCY 2 cut(s) 164, 232
RsaI GTAC 4 cut(s) 414, 564, 687, 1757
RsaNI GTAC 4 cut(s) 413, 563, 686, 1756
RseI CAYNNNNRTG 2 cut(s) 265, 987
SapI GCTCTTC 1 cut(s) 1272
SaqAI TTAA 1 cut(s) 1292
SatI GCNGC 2 cut(s) 291, 1116
Sau3AI GATC 4 cut(s) 164, 232, 432, 1580
Sau96I GGNCC 2 cut(s) 568, 1157
SchI GAGTC 3 cut(s) 567, 1204, 1569
ScrFI CCNGG 4 cut(s) 732, 801, 1155, 1196
SfaNI GCATC 3 cut(s) 355, 603, 1697
SfcI CTRYAG 1 cut(s) 1599
SfuI TTCGAA 1 cut(s) 141
SinI GGWCC 2 cut(s) 568, 1157
SmiMI CAYNNNNRTG 2 cut(s) 265, 987
Sse9I AATT 7 cut(s) 181, 219, 459, 809, 916, 1139, 1240
SsiI CCGC 7 cut(s) 290, 299, 465, 683, 1115, 1439, 1626
SspI AATATT 4 cut(s) 241, 714, 1462, 1500
SspMI CTAG 1 cut(s) 1229
StyD4I CCNGG 4 cut(s) 730, 799, 1153, 1194
TaaI ACNGT 3 cut(s) 835, 1188, 1724
TaiI ACGT 5 cut(s) 63, 250, 870, 1222, 1757
TaqI TCGA 1 cut(s) 141
TaqII GACCGA 1 cut(s) 585
TasI AATT 7 cut(s) 181, 219, 459, 809, 916, 1139, 1240
TauI GCSGC 2 cut(s) 293, 1118
TfiI GAWTC 2 cut(s) 1307, 1404
Tru1I TTAA 1 cut(s) 1292
Tru9I TTAA 1 cut(s) 1292
TscAI CASTG 2 cut(s) 1393, 1557
TseFI GTSAC 1 cut(s) 197
Tsp45I GTSAC 1 cut(s) 197
TspDTI ATGAA 9 cut(s) 272, 344, 623, 627, 683, 990, 1250, 1331, 1669
TspRI CASTG 2 cut(s) 1393, 1557
Van91I CCANNNNNTGG 1 cut(s) 1201
VpaK11BI GGWCC 2 cut(s) 568, 1157
XapI RAATTY 3 cut(s) 459, 916, 1240
XbaI TCTAGA 1 cut(s) 1228
XceI RCATGY 1 cut(s) 890
XmiI GTMKAC 1 cut(s) 1056
XmnI GAANNNNTTC 1 cut(s) 746
XspI CTAG 1 cut(s) 1229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.