Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0005290

Physical Location & Seq

Genomic Coordinates Forward (+)

37135021 .. 37140992

Gene Structure 5972 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1316900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2085 bp

ATGTTTTATTCGCAGACGTTCTTGGCTCGGAAGGGCCCACTGGGAACCGTCTGGTGCGCGGCCCACCTCCAGAGCCGGCTGAAGAAGTCCCACTATACTTCCACCGACATCCCCTCCACCGTTGATCGCATCATGTTTCCGGATGTTCCTATTGCACTGAGAATGTCAGGACACCTTTTATTTGGCGTTGTTCGGATATACTCCAAGCAGGTCGACTATCTTTACCAGGACTGTAATGTTGTTTTGACTACCCTAAGAAAGACTTTTGCTTCCATAGACTTGAATTTGCCAGAGAATGCAATGCAAGCTCCAGTTCAGTCCATCACTTTGCCGGAAACATTTGACCTTGATGCTTTGGATTTGGATGGAGGCGTGTTCGGTGAGGGGGCTTATGATAATCATCACGCGAGTCAGGAGGACATCACACTACCAGATCAAATTCCTATTGCAACAGACCGTTATGTCACTATAACTTTTGACGAGGATATCCAGAACGAAGTTTTTGGTTCAGATGTTGCTCCCATGGATGAGGACATCCACTCTACAACTCCAGGCGAGGGTGTTCCTAGTGATCGCTTCACCCCTCCAAATAAAGAAGCTGGAGTTATTCAAGACGCTCTTCCTGACAACAGTTCCCTTCAGTATGTTCAACCAACGGAAGTTCTGCAAGATGATGCTCATCTTCCAGATGACGGTTCCCCTCAACATGTTCAACCAATGGAAGTTATGCGAGATGCTGTTCCTCCAGAAATTGTTCCTATGGTTGCTCTCAATAACGGCAATGATCTTACTGAACCTGACAAATCTATTAACCCAGAAATGAATGAGAAAGAAGTACTTTCTCCAATTACAGAGGAGAATACTCCTTCTGGTGGGCCATCTCCATTGTCCCTGCCAAGTTCAGGACCACGAGCTTCTGTTGCTTCTTTGCCAGAAATGAATGAGAAAGATGTTTCCTTTCAAATGCAATCAACACCACATGTTGAGCAACCAAGAGCAAGAGCAAGGAAGAGAAAGCAGTATTTTGATGAGACCCTTGTGTTATCCAATGAGTTTATGAATAGGACTATTCAAGATGCTAGTGATTTAAAGCGTAAAAAGAGGAATATTGCCTACTCTACTTTGGGTGTTTGGAAGTTGAACAACAGTCTAAGAAAGGAACAAATATTTTTCCAGCCTTCATTAACTGGATTGTGTTCGGATCTTCGTAACATTTTTGACAAGGACTATATATCCAAACAGTCCCATTTGGTTGAGGGAGCTCTTCCAGTCTCTGGATCTGCAACATCTCCTCCTACAACTGAATTCTCCCCAGAGCGCGGTGTTCCACCATCTCTTCCAGTCATTGAAGTTTCCACAGAGTTTAATGGCGCAGAATCTCCCCCAACAATTGAACATTTTCAGCATGTTGAAGAACATGATGGTGGAAATAACTTACCAGAGCATGAGGGTGCACAATCTCCGCCACAAATTGAACGTTTTCAACATGTTGAAGAACATGATGGTGGAAATAACTTACCAGAGTATGAGGGTGCACAATCTCCTACCACTCAATATGACTTTATTCCTGCTCCGTCTCCAAACTTAATGTCAGCTTCAGTCCCTCCATTGGAAGCAAGCACTGGAACTCAAATACTGCTGACACCGGATCAAACAGCATCGACTAGATTTCGCGAATCTGTAAATGAAACATCAAAGACAATGTTTGAGGAGTGGGTAGGTCTGGAGAACACTGGTCTATCAAATAATCCTGAGTTCAACGATTCTGCTGAAGCAGGTCTTTTTTTCCTAGAAGCAGATGGCAATACACCAGCTGGATCTCAAGCGACACAAGGAATACCGGGATCTCAAGGGACACAAGGAGTTGATTCATTGTCAGTTAGAACCAGGGCTGTGGCTCAATTTTTGAAAAGGGAATCTTCTATCACCTCAACTTCAGAAGACGTCACTGGAGATCTCAGCTTGGACAAAATTTTAGAAGGAAAAACTAGAAAGCTATGTGCCCGAATGTTCTATGAAACACTGTCCTTCAAGTATTTGATGTGGCATGGATCTTATGGTTTTGAAGACTTATGGTCTTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000278 GO:0000793 GO:0000794 GO:0000798 GO:0000819 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007275 GO:0008150 GO:0008152 GO:0008278 GO:0009553 GO:0009555 GO:0009561 GO:0009987 GO:0016043 GO:0022402 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0050896 GO:0051276 GO:0051716 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0098813 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 map04110 map04111

Pfam Domains

Protein Families

Rad21_Rec8 Rad21_Rec8_N

Protein Analysis

695

Amino Acids

76.57

Weight (kDa)

4.6

Isoelectric Point (pI)

51.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad21_Rec8_N	PF04825	1 - 102	2.3e-36	N terminus of Rad21 / Rec8 like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005290)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59550 AT4G05360
fragaria_vesca	FvH4_7g00520 FvH4_7g00520 FvH4_7g00520 FvH4_7g00520 FvH4_7g00520 FvH4_7g00520 FvH4_7g00520
malus_domestica	MD02G1316900.v1.1 MD07G1005000.v1.1
prunus_persica	Prupe.2G007300_v2.0.a1 Prupe.2G007300_v2.0.a1 Prupe.2G007300_v2.0.a1 Prupe.2G007300_v2.0.a1
pyrus_communis	pycom07g00410
rosa_chinensis	RchiOBHm_Chr1g0314311
rosa_laevigata	RLG00000030767
rosa_multiflora	Rmu_sc0000343.1_g000013 Rmu_sc0002833.1_g000005 Rmu_sc0042953.1_g000001
rosa_roxburghii	Rroxscaffold_4G00331970
rosa_rugosa	Rorug01G0000900 Rorug01G0000900
rosa_samantha	Rh1AG010000 Rh1BG002900 Rh1CG010300 Rh1DG005400
rosa_wichuraiana	Rw1G000450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	461
AatII	GACGTC	1 cut(s)	1947
Acc36I	ACCTGC	2 cut(s)	199, 1766
AccB7I	CCANNNNNTGG	1 cut(s)	1274
AccI	GTMKAC	1 cut(s)	213
AccII	CGCG	4 cut(s)	59, 407, 1320, 1674
AccIII	TCCGGA	1 cut(s)	139
AciI	CCGC	3 cut(s)	59, 1320, 1463
AclI	AACGTT	1 cut(s)	1477
AclWI	GGATC	6 cut(s)	1209, 1285, 1656, 1825, 1852, 2059
AcsI	RAATTY	4 cut(s)	283, 438, 1304, 1971
AcuI	CTGAAG	5 cut(s)	101, 623, 1581, 1791, 1920
AcyI	GRCGYC	1 cut(s)	1944
AfaI	GTAC	1 cut(s)	837
AfiI	CCNNNNNNNGG	7 cut(s)	557, 692, 872, 902, 1274, 1319, 1609
AflIII	ACRYGT	3 cut(s)	706, 979, 1486
AhdI	GACNNNNNGTC	1 cut(s)	2074
AjnI	CCWGG	3 cut(s)	225, 550, 1886
AluBI	AGCT	8 cut(s)	308, 599, 914, 1262, 1595, 1814, 1962, 1996
AluI	AGCT	8 cut(s)	308, 599, 914, 1262, 1595, 1814, 1962, 1996
Alw21I	GWGCWC	3 cut(s)	1264, 1456, 1537
Alw26I	GTCTC	3 cut(s)	1025, 1276, 1581
Alw44I	GTGCAC	2 cut(s)	1452, 1533
AlwI	GGATC	6 cut(s)	1209, 1285, 1656, 1825, 1852, 2059
AlwNI	CAGNNNCTG	1 cut(s)	1274
Aor13HI	TCCGGA	1 cut(s)	139
AoxI	GGCC	3 cut(s)	34, 60, 875
ApaI	GGGCCC	1 cut(s)	38
ApaLI	GTGCAC	2 cut(s)	1452, 1533
ApoI	RAATTY	4 cut(s)	283, 438, 1304, 1971
ArsI	GACNNNNNNTTYG	2 cut(s)	1574, 1606
Asp700I	GAANNNNTTC	3 cut(s)	753, 1398, 1479
AspLEI	GCGC	3 cut(s)	59, 1320, 1373
AspS9I	GGNCC	5 cut(s)	34, 35, 61, 875, 905
AsuC2I	CCSGG	1 cut(s)	1842
AsuHPI	GGTGA	3 cut(s)	392, 571, 1918
AvaII	GGWCC	1 cut(s)	905
BaeGI	GKGCMC	4 cut(s)	38, 1456, 1537, 2005
BanII	GRGCYC	2 cut(s)	38, 1264
BauI	CACGAG	1 cut(s)	909
BbsI	GAAGAC	2 cut(s)	1947, 2073
Bbv12I	GWGCWC	3 cut(s)	1264, 1456, 1537
BccI	CCATC	7 cut(s)	329, 359, 886, 1339, 1415, 1496, 1793
BceAI	ACGGC	1 cut(s)	793
BciT130I	CCWGG	3 cut(s)	227, 552, 1888
BcnI	CCSGG	1 cut(s)	1842
BcoDI	GTCTC	3 cut(s)	1025, 1276, 1581
BfaI	CTAG	5 cut(s)	567, 1080, 1665, 1790, 1989
BfuAI	ACCTGC	2 cut(s)	199, 1766
BglII	AGATCT	1 cut(s)	1954
BisI	GCNGC	1 cut(s)	60
BlsI	GCNGC	1 cut(s)	61
BmcAI	AGTACT	1 cut(s)	837
Bme1390I	CCNGG	4 cut(s)	227, 552, 1842, 1888
Bme18I	GGWCC	1 cut(s)	905
BmeRI	GACNNNNNGTC	1 cut(s)	2074
BmgT120I	GGNCC	5 cut(s)	34, 35, 61, 875, 905
BmiI	GGNNCC	3 cut(s)	36, 46, 697
BmrFI	CCNGG	4 cut(s)	227, 552, 1842, 1888
BmrI	ACTGGG	1 cut(s)	50
BmsI	GCATC	6 cut(s)	138, 340, 664, 724, 1066, 1667
BmuI	ACTGGG	1 cut(s)	50
BpiI	GAAGAC	2 cut(s)	1947, 2073
BpmI	CTGGAG	7 cut(s)	53, 294, 534, 621, 729, 1745, 1971
BpuEI	CTTGAG	2 cut(s)	1806, 1833
BpuMI	CCSGG	1 cut(s)	1842
BsaBI	GATNNNNATC	2 cut(s)	399, 678
BsaHI	GRCGYC	1 cut(s)	1944
BsaI	GGTCTC	1 cut(s)	1025
BsaJI	CCNNGG	2 cut(s)	522, 1887
BsaWI	WCCGGW	2 cut(s)	139, 1645
BsaXI	ACNNNNNCTCC	4 cut(s)	98, 128, 1276, 1306
Bsc4I	CCNNNNNNNGG	7 cut(s)	557, 692, 872, 902, 1274, 1319, 1609
Bse118I	RCCGGY	1 cut(s)	75
Bse1I	ACTGG	8 cut(s)	45, 311, 1192, 1268, 1340, 1627, 1738, 1954
Bse3DI	GCAATG	2 cut(s)	306, 787
Bse8I	GATNNNNATC	2 cut(s)	399, 678
BseAI	TCCGGA	1 cut(s)	139
BseBI	CCWGG	3 cut(s)	227, 552, 1888
BseDI	CCNNGG	2 cut(s)	522, 1887
BseGI	GGATG	5 cut(s)	108, 148, 370, 532, 534
BseJI	GATNNNNATC	2 cut(s)	399, 678
BseLI	CCNNNNNNNGG	7 cut(s)	557, 692, 872, 902, 1274, 1319, 1609
BseMI	GCAATG	2 cut(s)	306, 787
BseMII	CTCAG	3 cut(s)	149, 1743, 1972
BseNI	ACTGG	8 cut(s)	45, 311, 1192, 1268, 1340, 1627, 1738, 1954
BseRI	GAGGAG	3 cut(s)	869, 1281, 1724
BseSI	GKGCMC	4 cut(s)	38, 1456, 1537, 2005
Bsh1236I	CGCG	4 cut(s)	59, 407, 1320, 1674
BshFI	GGCC	3 cut(s)	36, 62, 877
BsiHKAI	GWGCWC	3 cut(s)	1264, 1456, 1537
BsiSI	CCGG	5 cut(s)	76, 140, 332, 1646, 1841
BslFI	GGGAC	5 cut(s)	73, 874, 1228, 1586, 1867
BslI	CCNNNNNNNGG	7 cut(s)	557, 692, 872, 902, 1274, 1319, 1609
BsmAI	GTCTC	3 cut(s)	1025, 1276, 1581
BsmBI	CGTCTC	1 cut(s)	1581
BsmFI	GGGAC	5 cut(s)	73, 874, 1228, 1586, 1867
BsmI	GAATGC	1 cut(s)	301
BsnI	GGCC	3 cut(s)	36, 62, 877
Bso31I	GGTCTC	1 cut(s)	1025
Bsp120I	GGGCCC	1 cut(s)	34
Bsp1286I	GDGCHC	5 cut(s)	38, 1264, 1456, 1537, 2005
Bsp13I	TCCGGA	1 cut(s)	139
Bsp19I	CCATGG	1 cut(s)	522
Bsp68I	TCGCGA	1 cut(s)	1674
BspACI	CCGC	3 cut(s)	59, 1320, 1463
BspANI	GGCC	3 cut(s)	36, 62, 877
BspCNI	CTCAG	3 cut(s)	150, 1744, 1971
BspEI	TCCGGA	1 cut(s)	139
BspFNI	CGCG	4 cut(s)	59, 407, 1320, 1674
BspLI	GGNNCC	3 cut(s)	36, 46, 697
BspMI	ACCTGC	2 cut(s)	199, 1766
BspPI	GGATC	6 cut(s)	1209, 1285, 1656, 1825, 1852, 2059
BspQI	GCTCTTC	2 cut(s)	624, 1269
BspTNI	GGTCTC	1 cut(s)	1025
BsrDI	GCAATG	2 cut(s)	306, 787
BsrFI	RCCGGY	1 cut(s)	75
BsrI	ACTGG	8 cut(s)	45, 311, 1192, 1268, 1340, 1627, 1738, 1954
BssAI	RCCGGY	1 cut(s)	75
BssECI	CCNNGG	2 cut(s)	522, 1887
BssNI	GRCGYC	1 cut(s)	1944
BssSI	CACGAG	1 cut(s)	909
BssT1I	CCWWGG	1 cut(s)	522
Bst2BI	CACGAG	1 cut(s)	909
Bst2UI	CCWGG	3 cut(s)	227, 552, 1888
Bst4CI	ACNGT	9 cut(s)	49, 121, 233, 458, 632, 695, 1148, 1242, 2025
Bst6I	CTCTTC	4 cut(s)	624, 1004, 1269, 1341
BstACI	GRCGYC	1 cut(s)	1944
BstC8I	GCNNGC	3 cut(s)	77, 306, 1618
BstDEI	CTNAG	5 cut(s)	158, 254, 1151, 1752, 1958
BstDSI	CCRYGG	1 cut(s)	522
BstF5I	GGATG	5 cut(s)	108, 148, 370, 532, 534
BstFNI	CGCG	4 cut(s)	59, 407, 1320, 1674
BstHHI	GCGC	3 cut(s)	59, 1320, 1373
BstMAI	GTCTC	3 cut(s)	1025, 1276, 1581
BstMWI	GCNNNNNNNGC	2 cut(s)	305, 920
BstNI	CCWGG	3 cut(s)	227, 552, 1888
BstNSI	RCATGY	4 cut(s)	710, 983, 1409, 1490
BstSCI	CCNGG	4 cut(s)	225, 550, 1840, 1886
BstSLI	GKGCMC	4 cut(s)	38, 1456, 1537, 2005
BstUI	CGCG	4 cut(s)	59, 407, 1320, 1674
BstV2I	GAAGAC	2 cut(s)	1947, 2073
BstX2I	RGATCY	6 cut(s)	1201, 1277, 1817, 1844, 1954, 2051
BstXI	CCANNNNNNTGG	1 cut(s)	1894
BstYI	RGATCY	6 cut(s)	1201, 1277, 1817, 1844, 1954, 2051
BsuRI	GGCC	3 cut(s)	36, 62, 877
BtgI	CCRYGG	1 cut(s)	522
BtsCI	GGATG	5 cut(s)	108, 148, 370, 532, 534
BtsIMutI	CAGTG	6 cut(s)	38, 155, 1620, 1731, 1947, 2021
BtuMI	TCGCGA	1 cut(s)	1674
BveI	ACCTGC	2 cut(s)	199, 1766
Cac8I	GCNNGC	3 cut(s)	77, 306, 1618
CaiI	CAGNNNCTG	1 cut(s)	1274
CfoI	GCGC	3 cut(s)	59, 1320, 1373
Cfr10I	RCCGGY	1 cut(s)	75
Cfr13I	GGNCC	5 cut(s)	34, 35, 61, 875, 905
CseI	GACGC	1 cut(s)	623
Csp6I	GTAC	1 cut(s)	836
CviQI	GTAC	1 cut(s)	836
DdeI	CTNAG	5 cut(s)	158, 254, 1151, 1752, 1958
DraI	TTTAAA	1 cut(s)	1089
DrdI	GACNNNNNNGTC	1 cut(s)	461
DriI	GACNNNNNGTC	1 cut(s)	2074
DseDI	GACNNNNNNGTC	1 cut(s)	461
Eam1104I	CTCTTC	4 cut(s)	624, 1004, 1269, 1341
Eam1105I	GACNNNNNGTC	1 cut(s)	2074
EarI	CTCTTC	4 cut(s)	624, 1004, 1269, 1341
EciI	GGCGGA	1 cut(s)	1452
Ecl136II	GAGCTC	1 cut(s)	1262
Eco130I	CCWWGG	1 cut(s)	522
Eco24I	GRGCYC	2 cut(s)	38, 1264
Eco31I	GGTCTC	1 cut(s)	1025
Eco32I	GATATC	1 cut(s)	487
Eco47I	GGWCC	1 cut(s)	905
Eco53kI	GAGCTC	1 cut(s)	1262
Eco57I	CTGAAG	5 cut(s)	101, 623, 1581, 1791, 1920
EcoICRI	GAGCTC	1 cut(s)	1262
EcoO109I	RGGNCCY	1 cut(s)	34
EcoRI	GAATTC	1 cut(s)	1304
EcoRII	CCWGG	3 cut(s)	225, 550, 1886
EcoRV	GATATC	1 cut(s)	487
EcoT14I	CCWWGG	1 cut(s)	522
EcoT38I	GRGCYC	2 cut(s)	38, 1264
ErhI	CCWWGG	1 cut(s)	522
Esp3I	CGTCTC	1 cut(s)	1581
FaqI	GGGAC	5 cut(s)	73, 874, 1228, 1586, 1867
FblI	GTMKAC	1 cut(s)	213
Fnu4HI	GCNGC	1 cut(s)	60
FokI	GGATG	5 cut(s)	95, 155, 377, 521, 539
FriOI	GRGCYC	2 cut(s)	38, 1264
Fsp4HI	GCNGC	1 cut(s)	60
FspBI	CTAG	5 cut(s)	567, 1080, 1665, 1790, 1989
GlaI	GCGC	3 cut(s)	58, 1319, 1372
GluI	GCNGC	1 cut(s)	60
GsuI	CTGGAG	7 cut(s)	53, 294, 534, 621, 729, 1745, 1971
HaeIII	GGCC	3 cut(s)	36, 62, 877
HapII	CCGG	5 cut(s)	76, 140, 332, 1646, 1841
HgaI	GACGC	1 cut(s)	623
HhaI	GCGC	3 cut(s)	59, 1320, 1373
Hin1I	GRCGYC	1 cut(s)	1944
Hin6I	GCGC	3 cut(s)	57, 1318, 1371
HinP1I	GCGC	3 cut(s)	57, 1318, 1371
HincII	GTYRAC	1 cut(s)	214
HindII	GTYRAC	1 cut(s)	214
HinfI	GANTC	6 cut(s)	409, 1376, 1676, 1763, 1868, 1916
HpaII	CCGG	5 cut(s)	76, 140, 332, 1646, 1841
HphI	GGTGA	3 cut(s)	392, 571, 1918
Hpy166II	GTNNAC	3 cut(s)	214, 1454, 1535
Hpy188I	TCNGA	5 cut(s)	30, 195, 511, 1201, 1939
Hpy8I	GTNNAC	3 cut(s)	214, 1454, 1535
HpyAV	CCTTC	6 cut(s)	25, 647, 876, 1188, 1973, 2038
HpyCH4III	ACNGT	9 cut(s)	49, 121, 233, 458, 632, 695, 1148, 1242, 2025
HpyCH4IV	ACGT	3 cut(s)	17, 1477, 1944
HpyCH4V	TGCA	9 cut(s)	155, 299, 304, 449, 667, 967, 1283, 1454, 1535
HpyF10VI	GCNNNNNNNGC	2 cut(s)	305, 920
HpyF3I	CTNAG	5 cut(s)	158, 254, 1151, 1752, 1958
HpySE526I	ACGT	3 cut(s)	17, 1477, 1944
Hsp92I	GRCGYC	1 cut(s)	1944
HspAI	GCGC	3 cut(s)	57, 1318, 1371
Kpn2I	TCCGGA	1 cut(s)	139
KroI	GCCGGC	1 cut(s)	75
KroNI	GCCGGC	1 cut(s)	77
LguI	GCTCTTC	2 cut(s)	624, 1269
LmnI	GCTCC	4 cut(s)	313, 523, 1259, 1576
LweI	GCATC	6 cut(s)	138, 340, 664, 724, 1066, 1667
MaeI	CTAG	5 cut(s)	567, 1080, 1665, 1790, 1989
MaeII	ACGT	3 cut(s)	17, 1477, 1944
MaeIII	GTNAC	3 cut(s)	463, 1208, 1945
MfeI	CAATTG	1 cut(s)	1389
MflI	RGATCY	6 cut(s)	1201, 1277, 1817, 1844, 1954, 2051
MhlI	GDGCHC	5 cut(s)	38, 1264, 1456, 1537, 2005
MluCI	AATT	9 cut(s)	283, 438, 750, 846, 1304, 1389, 1470, 1901, 1971
MlyI	GAGTC	1 cut(s)	418
MroI	TCCGGA	1 cut(s)	139
MroNI	GCCGGC	1 cut(s)	75
MroXI	GAANNNNTTC	3 cut(s)	753, 1398, 1479
MseI	TTAA	5 cut(s)	810, 1088, 1184, 1365, 1586
MslI	CAYNNNNRTG	3 cut(s)	1422, 1449, 1503
MspA1I	CMGCKG	1 cut(s)	1814
MspI	CCGG	5 cut(s)	76, 140, 332, 1646, 1841
MspR9I	CCNGG	4 cut(s)	227, 552, 1842, 1888
MunI	CAATTG	1 cut(s)	1389
Mva1269I	GAATGC	1 cut(s)	301
MvaI	CCWGG	3 cut(s)	227, 552, 1888
MvnI	CGCG	4 cut(s)	59, 407, 1320, 1674
MwoI	GCNNNNNNNGC	2 cut(s)	305, 920
NaeI	GCCGGC	1 cut(s)	77
NciI	CCSGG	1 cut(s)	1842
NcoI	CCATGG	1 cut(s)	522
NgoMIV	GCCGGC	1 cut(s)	75
NlaIV	GGNNCC	3 cut(s)	36, 46, 697
NmuCI	GTSAC	2 cut(s)	463, 1945
NruI	TCGCGA	1 cut(s)	1674
NspI	RCATGY	4 cut(s)	710, 983, 1409, 1490
PciI	ACATGT	3 cut(s)	706, 979, 1486
PciSI	GCTCTTC	2 cut(s)	624, 1269
PctI	GAATGC	1 cut(s)	301
PdiI	GCCGGC	1 cut(s)	77
PdmI	GAANNNNTTC	3 cut(s)	753, 1398, 1479
PfeI	GAWTC	5 cut(s)	1376, 1676, 1763, 1868, 1916
PflMI	CCANNNNNTGG	1 cut(s)	1274
PkrI	GCNGC	1 cut(s)	61
PleI	GAGTC	1 cut(s)	417
PpsI	GAGTC	1 cut(s)	417
PscI	ACATGT	3 cut(s)	706, 979, 1486
Psp124BI	GAGCTC	1 cut(s)	1264
Psp1406I	AACGTT	1 cut(s)	1477
Psp6I	CCWGG	3 cut(s)	225, 550, 1886
PspGI	CCWGG	3 cut(s)	225, 550, 1886
PspN4I	GGNNCC	3 cut(s)	36, 46, 697
PspOMI	GGGCCC	1 cut(s)	34
PspPI	GGNCC	5 cut(s)	34, 35, 61, 875, 905
PstNI	CAGNNNCTG	1 cut(s)	1274
PsuI	RGATCY	6 cut(s)	1201, 1277, 1817, 1844, 1954, 2051
PvuII	CAGCTG	1 cut(s)	1814
RruI	TCGCGA	1 cut(s)	1674
RsaI	GTAC	1 cut(s)	837
RsaNI	GTAC	1 cut(s)	836
RseI	CAYNNNNRTG	3 cut(s)	1422, 1449, 1503
SacI	GAGCTC	1 cut(s)	1264
SalI	GTCGAC	1 cut(s)	212
SapI	GCTCTTC	2 cut(s)	624, 1269
SaqAI	TTAA	5 cut(s)	810, 1088, 1184, 1365, 1586
SatI	GCNGC	1 cut(s)	60
Sau96I	GGNCC	5 cut(s)	34, 35, 61, 875, 905
ScaI	AGTACT	1 cut(s)	837
SchI	GAGTC	1 cut(s)	418
ScrFI	CCNGG	4 cut(s)	227, 552, 1842, 1888
SduI	GDGCHC	5 cut(s)	38, 1264, 1456, 1537, 2005
SfaNI	GCATC	6 cut(s)	138, 340, 664, 724, 1066, 1667
SinI	GGWCC	1 cut(s)	905
SmiMI	CAYNNNNRTG	3 cut(s)	1422, 1449, 1503
SmlI	CTYRAG	2 cut(s)	1821, 1848
SmoI	CTYRAG	2 cut(s)	1821, 1848
Sse9I	AATT	9 cut(s)	283, 438, 750, 846, 1304, 1389, 1470, 1901, 1971
SsiI	CCGC	3 cut(s)	59, 1320, 1463
SspI	AATATT	2 cut(s)	1108, 1167
SspMI	CTAG	5 cut(s)	567, 1080, 1665, 1790, 1989
SstI	GAGCTC	1 cut(s)	1264
StyD4I	CCNGG	4 cut(s)	225, 550, 1840, 1886
StyI	CCWWGG	1 cut(s)	522
TaaI	ACNGT	9 cut(s)	49, 121, 233, 458, 632, 695, 1148, 1242, 2025
TaiI	ACGT	3 cut(s)	20, 1480, 1947
TaqI	TCGA	2 cut(s)	213, 1661
TasI	AATT	9 cut(s)	283, 438, 750, 846, 1304, 1389, 1470, 1901, 1971
TatI	WGTACW	1 cut(s)	835
TauI	GCSGC	1 cut(s)	62
TfiI	GAWTC	5 cut(s)	1376, 1676, 1763, 1868, 1916
Tru1I	TTAA	5 cut(s)	810, 1088, 1184, 1365, 1586
Tru9I	TTAA	5 cut(s)	810, 1088, 1184, 1365, 1586
TscAI	CASTG	6 cut(s)	45, 162, 1627, 1738, 1954, 2028
TseFI	GTSAC	2 cut(s)	463, 1945
Tsp45I	GTSAC	2 cut(s)	463, 1945
TspDTI	ATGAA	7 cut(s)	836, 953, 1073, 1170, 1701, 1860, 2031
TspGWI	ACGGA	2 cut(s)	671, 1563
TspRI	CASTG	6 cut(s)	45, 162, 1627, 1738, 1954, 2028
Van91I	CCANNNNNTGG	1 cut(s)	1274
VneI	GTGCAC	2 cut(s)	1452, 1533
VpaK11BI	GGWCC	1 cut(s)	905
XapI	RAATTY	4 cut(s)	283, 438, 1304, 1971
XceI	RCATGY	4 cut(s)	710, 983, 1409, 1490
XmiI	GTMKAC	1 cut(s)	213
XmnI	GAANNNNTTC	3 cut(s)	753, 1398, 1479
XspI	CTAG	5 cut(s)	567, 1080, 1665, 1790, 1989
ZraI	GACGTC	1 cut(s)	1945
ZrmI	AGTACT	1 cut(s)	837

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.