MD03G1003200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013061
Physical Location & Seq
Reverse (-)
227813 .. 229983
2171 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1003200.v1.1.491

Sequence Viewer

Length: 507 bp
ATGTGGTGTGATCCTAAGGAAGGCAGCCAGGCGCACACACACACACATATATATATATTGATGAGGTTTGTGTTTTGCAAGATTCATTGTCCTCCCTTCATCTGCTTTTGCAAGCCTAATTCCCCTCATATATACACTCCTGGACCACTCAAATTACAGAATTCCCCAACTCCATCTCCACCTTTGCCTAATTCAAGACTTGAACCAGTTCCTGAGACATCTGACCACTTATCTGATGAAGAAACCACTGAGATCATCAAGGAGGAGAGGTTATTAGGTAATGCGAATCCACAACCAGCTACCGCTCAGCCTTGTCTCAAGAGTAGTCTCCGAAAGCCTAATTCCGACTCAGGTGCTCCAAAACAGGAGGTGCTCAAGAAGAGAGTGCAGTGGACAGACTTTTTAGGGAAGCAGCTTGTTGACGTTAGGGAATTTGAGTGCAGTGAACCAGAAGATACCGACAATGAAGACGACAACAGAAGAGGCTGCATTTGTGTTATTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

19.03

Weight (kDa)

5.67

Isoelectric Point (pI)

64.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013061)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13480 AT3G13480
fragaria_vesca FvH4_3g45780
malus_domestica MD03G1003200.v1.1 MD11G1003000.v1.1
prunus_persica Prupe.6G002800_v2.0.a1 Prupe.6G002800_v2.0.a1
pyrus_communis pycom03g00320
rosa_chinensis RchiOBHm_Chr5g0083851
rosa_laevigata RLG00000037058
rosa_multiflora Rmu_sc0001287.1_g000041
rosa_roxburghii Rroxscaffold_1G00000740
rosa_rugosa Rorug05G0486600
rosa_samantha Rh5AG539700 Rh5BG564800 Rh5CG586700 Rh5DG573300
rosa_wichuraiana Rw5G050180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 305
AciI CCGC 1 cut(s) 303
AclWI GGATC 1 cut(s) 5
AcsI RAATTY 2 cut(s) 160, 431
AfiI CCNNNNNNNGG 1 cut(s) 20
AgsI TTSAA 2 cut(s) 195, 203
AjnI CCWGG 2 cut(s) 27, 139
AluBI AGCT 2 cut(s) 299, 415
AluI AGCT 2 cut(s) 299, 415
Alw21I GWGCWC 2 cut(s) 358, 375
Alw26I GTCTC 3 cut(s) 209, 320, 332
AlwI GGATC 1 cut(s) 5
AlwNI CAGNNNCTG 1 cut(s) 212
ApeKI GCWGC 3 cut(s) 24, 412, 486
ApoI RAATTY 2 cut(s) 160, 431
Asp700I GAANNNNTTC 1 cut(s) 207
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 1 cut(s) 143
AvaII GGWCC 1 cut(s) 143
AxyI CCTNAGG 1 cut(s) 15
BbsI GAAGAC 1 cut(s) 474
Bbv12I GWGCWC 2 cut(s) 358, 375
BbvI GCAGC 3 cut(s) 36, 424, 473
BccI CCATC 1 cut(s) 181
BcgI CGANNNNNNTGC 2 cut(s) 335, 369
BciT130I CCWGG 2 cut(s) 29, 141
BcoDI GTCTC 3 cut(s) 209, 320, 332
BisI GCNGC 3 cut(s) 25, 413, 487
BlpI GCTNAGC 1 cut(s) 306
BlsI GCNGC 3 cut(s) 26, 414, 488
Bme1390I CCNGG 2 cut(s) 29, 141
Bme18I GGWCC 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 143
BmrFI CCNGG 2 cut(s) 29, 141
BpiI GAAGAC 1 cut(s) 474
Bpu1102I GCTNAGC 1 cut(s) 306
BpuEI CTTGAG 2 cut(s) 302, 359
BsaXI ACNNNNNCTCC 4 cut(s) 160, 190, 254, 284
Bsc4I CCNNNNNNNGG 1 cut(s) 20
Bse1I ACTGG 1 cut(s) 206
Bse21I CCTNAGG 1 cut(s) 15
BseBI CCWGG 2 cut(s) 29, 141
BseLI CCNNNNNNNGG 1 cut(s) 20
BseMII CTCAG 4 cut(s) 204, 240, 320, 363
BseNI ACTGG 1 cut(s) 206
BseRI GAGGAG 1 cut(s) 278
BseXI GCAGC 3 cut(s) 36, 424, 473
BsgI GTGCAG 2 cut(s) 407, 460
BsiHKAI GWGCWC 2 cut(s) 358, 375
BslI CCNNNNNNNGG 1 cut(s) 20
BsmAI GTCTC 3 cut(s) 209, 320, 332
Bsp1286I GDGCHC 2 cut(s) 358, 375
Bsp143I GATC 2 cut(s) 10, 252
Bsp1720I GCTNAGC 1 cut(s) 306
BspACI CCGC 1 cut(s) 303
BspCNI CTCAG 4 cut(s) 205, 241, 319, 362
BspPI GGATC 1 cut(s) 5
BsrBI CCGCTC 1 cut(s) 305
BsrI ACTGG 1 cut(s) 206
BssMI GATC 2 cut(s) 10, 252
Bst2UI CCWGG 2 cut(s) 29, 141
Bst6I CTCTTC 2 cut(s) 374, 475
BstC8I GCNNGC 1 cut(s) 113
BstDEI CTNAG 5 cut(s) 15, 213, 249, 306, 349
BstENI CCTNNNNNAGG 1 cut(s) 18
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 2 cut(s) 13, 255
BstMAI GTCTC 3 cut(s) 209, 320, 332
BstMBI GATC 2 cut(s) 10, 252
BstNI CCWGG 2 cut(s) 29, 141
BstSCI CCNGG 2 cut(s) 27, 139
BstV1I GCAGC 3 cut(s) 36, 424, 473
BstV2I GAAGAC 1 cut(s) 474
Bsu36I CCTNAGG 1 cut(s) 15
BtsI GCAGTG 2 cut(s) 395, 448
BtsIMutI CAGTG 3 cut(s) 246, 395, 448
Cac8I GCNNGC 1 cut(s) 113
CaiI CAGNNNCTG 1 cut(s) 212
CfoI GCGC 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 143
CviJI RGCY 7 cut(s) 27, 115, 299, 310, 337, 415, 486
CviKI_1 RGCY 7 cut(s) 27, 115, 299, 310, 337, 415, 486
DdeI CTNAG 5 cut(s) 15, 213, 249, 306, 349
DpnI GATC 2 cut(s) 12, 254
DpnII GATC 2 cut(s) 10, 252
Eam1104I CTCTTC 2 cut(s) 374, 475
EarI CTCTTC 2 cut(s) 374, 475
Eco47I GGWCC 1 cut(s) 143
Eco81I CCTNAGG 1 cut(s) 15
EcoNI CCTNNNNNAGG 1 cut(s) 18
EcoRI GAATTC 1 cut(s) 160
EcoRII CCWGG 2 cut(s) 27, 139
FaiI YATR 9 cut(s) 48, 50, 52, 54, 56, 129, 131, 133, 505
Fnu4HI GCNGC 3 cut(s) 25, 413, 487
Fsp4HI GCNGC 3 cut(s) 25, 413, 487
GlaI GCGC 1 cut(s) 33
GluI GCNGC 3 cut(s) 25, 413, 487
HhaI GCGC 1 cut(s) 34
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HinfI GANTC 3 cut(s) 82, 286, 347
Hpy166II GTNNAC 3 cut(s) 393, 421, 446
Hpy188I TCNGA 4 cut(s) 223, 235, 332, 346
Hpy188III TCNNGA 4 cut(s) 195, 212, 319, 376
Hpy8I GTNNAC 3 cut(s) 393, 421, 446
HpyAV CCTTC 2 cut(s) 14, 106
HpyCH4IV ACGT 1 cut(s) 423
HpyCH4V TGCA 5 cut(s) 78, 111, 388, 441, 489
HpyF3I CTNAG 5 cut(s) 15, 213, 249, 306, 349
HpySE526I ACGT 1 cut(s) 423
HspAI GCGC 1 cut(s) 32
Kzo9I GATC 2 cut(s) 10, 252
LmnI GCTCC 1 cut(s) 361
Lsp1109I GCAGC 3 cut(s) 36, 424, 473
MaeII ACGT 1 cut(s) 423
MalI GATC 2 cut(s) 12, 254
MbiI CCGCTC 1 cut(s) 305
MboI GATC 2 cut(s) 10, 252
MboII GAAGA 5 cut(s) 251, 391, 464, 479, 492
MhlI GDGCHC 2 cut(s) 358, 375
MluCI AATT 6 cut(s) 118, 152, 160, 190, 340, 431
MlyI GAGTC 1 cut(s) 341
MmeI TCCRAC 1 cut(s) 369
MnlI CCTC 7 cut(s) 57, 102, 135, 256, 261, 361, 476
MroXI GAANNNNTTC 1 cut(s) 207
MspR9I CCNGG 2 cut(s) 29, 141
MvaI CCWGG 2 cut(s) 29, 141
NdeII GATC 2 cut(s) 10, 252
PdmI GAANNNNTTC 1 cut(s) 207
PfeI GAWTC 2 cut(s) 82, 286
PfoI TCCNGGA 1 cut(s) 139
PkrI GCNGC 3 cut(s) 26, 414, 488
PleI GAGTC 1 cut(s) 341
PpsI GAGTC 1 cut(s) 341
Psp6I CCWGG 2 cut(s) 27, 139
PspGI CCWGG 2 cut(s) 27, 139
PspPI GGNCC 1 cut(s) 143
PstNI CAGNNNCTG 1 cut(s) 212
SatI GCNGC 3 cut(s) 25, 413, 487
Sau3AI GATC 2 cut(s) 10, 252
Sau96I GGNCC 1 cut(s) 143
SchI GAGTC 1 cut(s) 341
ScrFI CCNGG 2 cut(s) 29, 141
SduI GDGCHC 2 cut(s) 358, 375
SetI ASST 9 cut(s) 68, 184, 272, 280, 301, 355, 372, 417, 426
SinI GGWCC 1 cut(s) 143
SmlI CTYRAG 2 cut(s) 317, 374
SmoI CTYRAG 2 cut(s) 317, 374
Sse9I AATT 6 cut(s) 118, 152, 160, 190, 340, 431
SsiI CCGC 1 cut(s) 303
StyD4I CCNGG 2 cut(s) 27, 139
TaiI ACGT 1 cut(s) 426
TasI AATT 6 cut(s) 118, 152, 160, 190, 340, 431
TfiI GAWTC 2 cut(s) 82, 286
TscAI CASTG 3 cut(s) 253, 395, 448
TseI GCWGC 3 cut(s) 24, 412, 486
TspDTI ATGAA 4 cut(s) 74, 88, 252, 480
TspRI CASTG 3 cut(s) 253, 395, 448
VpaK11BI GGWCC 1 cut(s) 143
XagI CCTNNNNNAGG 1 cut(s) 18
XapI RAATTY 2 cut(s) 160, 431
XmnI GAANNNNTTC 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.