Rorug05G0486600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0013061
Physical Location & Seq
Forward (+)
67023128 .. 67024595
1468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0486600.1

Sequence Viewer

Length: 1125 bp
ATGGTGTTCTCGCCGCTTTCCCTCCACATCGTTCTGAGCCTGATAGCTTCTGGGACAAAGGGCCCTTGTCTGGATCAGTTTCTCTCTTTCCTCAAGTCCAGATCCACCCACCACCTCAACTCCCTCGCTCATGACCTCGTCACTTTCGTTTTAGCCGATGGATCACCCGGCAGTGACGACAGTATTCATCCCGGCGGTCCTGGTCCTCGTTTGTCCTTTGCCAATGGCGTTTGGGTCGACGAGTCTCTTCCTTTCAAGCCTTATTTCAAACATGTAGTGGACACTGCTTACATGGCGGCTCTAAAGGAGGTCAACTTCAAGTCTAACCCTGACCAAGTAGGTATGGAAGTGAATTCTTGGGCTGAGAAGGAGACTAACGGCCTCATCAAAGAGATTCTTCCTCCAAATTCAGTCGACATCTACACGAAGCTCATCTTTGCAAATGCTTTATACTTCAAAGCATATTGGGTTAATAAGTTTTTTGATACAGAAACAAAAAAGCGTAACTTCTACCTTCTGGATGGGAGCTCGGTTAAAGCTGTTCCTTTTATGAGAGGCGGGGGCTACCAGTATATACGTGCTTTTAAAGGCTTCAAAGTCTTGAGGCTTCCTTACCGACAAATTGGAGTTTACCCCCGGTGTTTCTCTATGTACTTTTACCTTCCCGATGCAAGAGATGGGCTGCCAGCTTTGGTTGAGAGAGTTTGTTCTGAATCTGGATTTTTAGATAGCCATGTTCCCCATAATAAGCTTTATGTGGGTAAAATGTCAATCCCAAAGTTTAAGATTTCTTCTGGTTTTGAAGCTTCAGAAGTCATGAAGAGGTCAGGCCTGGTGCTGCCTTTCACTTTTGATCCTCTTAAAGGAGGTAATGTGACAGAGATGGTGGAGTCTCCTGCCGGTGAGGACCCATACGTCTCGGCAATATTTCATAAGTCGTGTATCGAAATTGATGAAGAAGGTACGGTAGCTTCAGCTGTTACTGTTGCTACGCTTTCTGGTGGGGGTGCTCCTCCTGTGGAAATAGACTTTGTGGCGAATCACCCATTCATGTTTTTCATCAGAGAAGACAGTACCGGAACAGTGTTGTTCATGGGGCAAGTGCTCAATCCCCTTGCTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

374

Amino Acids

41.24

Weight (kDa)

6.37

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serpin PF00079 1 - 371 1.5e-77 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013061)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13480 AT3G13480
fragaria_vesca FvH4_3g45780
malus_domestica MD03G1003200.v1.1 MD11G1003000.v1.1
prunus_persica Prupe.6G002800_v2.0.a1 Prupe.6G002800_v2.0.a1
pyrus_communis pycom03g00320
rosa_chinensis RchiOBHm_Chr5g0083851
rosa_laevigata RLG00000037058
rosa_multiflora Rmu_sc0001287.1_g000041
rosa_roxburghii Rroxscaffold_1G00000740
rosa_rugosa Rorug05G0486600
rosa_samantha Rh5AG539700 Rh5BG564800 Rh5CG586700 Rh5DG573300
rosa_wichuraiana Rw5G050180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 914
AccI GTMKAC 2 cut(s) 237, 414
AciI CCGC 4 cut(s) 14, 195, 296, 558
AclWI GGATC 4 cut(s) 81, 96, 169, 848
AcsI RAATTY 2 cut(s) 352, 406
AcuI CTGAAG 2 cut(s) 792, 957
AfaI GTAC 3 cut(s) 653, 964, 1075
AfiI CCNNNNNNNGG 3 cut(s) 70, 863, 1118
AflIII ACRYGT 1 cut(s) 271
AgsI TTSAA 6 cut(s) 256, 268, 319, 457, 595, 803
AjnI CCWGG 2 cut(s) 199, 831
AluBI AGCT 9 cut(s) 47, 430, 528, 539, 689, 751, 806, 971, 977
AluI AGCT 9 cut(s) 47, 430, 528, 539, 689, 751, 806, 971, 977
Alw21I GWGCWC 3 cut(s) 530, 1012, 1107
Alw26I GTCTC 4 cut(s) 249, 365, 897, 922
AlwI GGATC 4 cut(s) 81, 96, 169, 848
AoxI GGCC 3 cut(s) 61, 379, 829
ApaI GGGCCC 1 cut(s) 65
ApeKI GCWGC 2 cut(s) 682, 838
ApoI RAATTY 2 cut(s) 352, 406
AspS9I GGNCC 5 cut(s) 61, 62, 197, 203, 907
AsuC2I CCSGG 3 cut(s) 168, 192, 637
AsuHPI GGTGA 3 cut(s) 156, 914, 1034
AvaII GGWCC 3 cut(s) 197, 203, 907
BaeGI GKGCMC 1 cut(s) 65
BanII GRGCYC 2 cut(s) 65, 530
BarI GAAGNNNNNNTAC 2 cut(s) 955, 987
BbsI GAAGAC 1 cut(s) 1074
Bbv12I GWGCWC 3 cut(s) 530, 1012, 1107
BbvI GCAGC 2 cut(s) 669, 825
BccI CCATC 4 cut(s) 152, 515, 671, 877
BceAI ACGGC 1 cut(s) 394
BciT130I CCWGG 2 cut(s) 201, 833
BcnI CCSGG 3 cut(s) 168, 192, 637
BcoDI GTCTC 4 cut(s) 249, 365, 897, 922
BisI GCNGC 4 cut(s) 14, 297, 683, 839
BlsI GCNGC 4 cut(s) 15, 298, 684, 840
Bme1390I CCNGG 5 cut(s) 168, 192, 201, 637, 833
Bme18I GGWCC 3 cut(s) 197, 203, 907
BmgT120I GGNCC 5 cut(s) 61, 62, 197, 203, 907
BmiI GGNNCC 2 cut(s) 63, 909
BmrFI CCNGG 5 cut(s) 168, 192, 201, 637, 833
BmsI GCATC 1 cut(s) 658
BpiI GAAGAC 1 cut(s) 1074
BpuEI CTTGAG 2 cut(s) 77, 622
BpuMI CCSGG 3 cut(s) 168, 192, 637
BsaAI YACGTR 1 cut(s) 578
BsaJI CCNNGG 1 cut(s) 635
BsaWI WCCGGW 1 cut(s) 1076
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 858, 888
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 863, 1118
Bse118I RCCGGY 1 cut(s) 899
Bse1I ACTGG 1 cut(s) 568
BseBI CCWGG 2 cut(s) 201, 833
BseDI CCNNGG 1 cut(s) 635
BseGI GGATG 2 cut(s) 187, 526
BseLI CCNNNNNNNGG 3 cut(s) 70, 863, 1118
BseMII CTCAG 2 cut(s) 26, 354
BseNI ACTGG 1 cut(s) 568
BseRI GAGGAG 1 cut(s) 1002
BseSI GKGCMC 1 cut(s) 65
BseXI GCAGC 2 cut(s) 669, 825
BshFI GGCC 3 cut(s) 63, 381, 831
BsiHKAI GWGCWC 3 cut(s) 530, 1012, 1107
BsiSI CCGG 5 cut(s) 168, 192, 637, 900, 1077
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 3 cut(s) 70, 863, 1118
BsmAI GTCTC 4 cut(s) 249, 365, 897, 922
BsmBI CGTCTC 1 cut(s) 922
BsmFI GGGAC 1 cut(s) 67
BsnI GGCC 3 cut(s) 63, 381, 831
Bsp120I GGGCCC 1 cut(s) 61
Bsp1286I GDGCHC 4 cut(s) 65, 530, 1012, 1107
Bsp143I GATC 4 cut(s) 73, 101, 161, 853
BspACI CCGC 4 cut(s) 14, 195, 296, 558
BspANI GGCC 3 cut(s) 63, 381, 831
BspCNI CTCAG 2 cut(s) 27, 355
BspHI TCATGA 2 cut(s) 130, 816
BspLI GGNNCC 2 cut(s) 63, 909
BspPI GGATC 4 cut(s) 81, 96, 169, 848
BsrFI RCCGGY 1 cut(s) 899
BsrI ACTGG 1 cut(s) 568
BssAI RCCGGY 1 cut(s) 899
BssECI CCNNGG 1 cut(s) 635
BssMI GATC 4 cut(s) 73, 101, 161, 853
Bst2UI CCWGG 2 cut(s) 201, 833
Bst4CI ACNGT 5 cut(s) 182, 967, 985, 1073, 1084
Bst6I CTCTTC 2 cut(s) 252, 815
BstBAI YACGTR 1 cut(s) 578
BstC8I GCNNGC 2 cut(s) 687, 1120
BstDEI CTNAG 2 cut(s) 35, 363
BstENI CCTNNNNNAGG 1 cut(s) 861
BstF5I GGATG 2 cut(s) 187, 526
BstKTI GATC 4 cut(s) 76, 104, 164, 856
BstMAI GTCTC 4 cut(s) 249, 365, 897, 922
BstMBI GATC 4 cut(s) 73, 101, 161, 853
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 2 cut(s) 201, 833
BstNSI RCATGY 1 cut(s) 275
BstSCI CCNGG 5 cut(s) 166, 190, 199, 635, 831
BstSLI GKGCMC 1 cut(s) 65
BstV1I GCAGC 2 cut(s) 669, 825
BstV2I GAAGAC 1 cut(s) 1074
BstX2I RGATCY 1 cut(s) 101
BstYI RGATCY 1 cut(s) 101
BsuRI GGCC 3 cut(s) 63, 381, 831
BtsCI GGATG 2 cut(s) 187, 526
BtsI GCAGTG 2 cut(s) 178, 282
BtsIMutI CAGTG 3 cut(s) 178, 282, 1089
Cac8I GCNNGC 2 cut(s) 687, 1120
CciI TCATGA 2 cut(s) 130, 816
Cfr10I RCCGGY 1 cut(s) 899
Cfr13I GGNCC 5 cut(s) 61, 62, 197, 203, 907
Csp6I GTAC 3 cut(s) 652, 963, 1074
CviAII CATG 7 cut(s) 131, 272, 292, 734, 817, 1051, 1093
CviQI GTAC 3 cut(s) 652, 963, 1074
DdeI CTNAG 2 cut(s) 35, 363
DpnI GATC 4 cut(s) 75, 103, 163, 855
DpnII GATC 4 cut(s) 73, 101, 161, 853
DraI TTTAAA 1 cut(s) 586
DrdI GACNNNNNNGTC 1 cut(s) 914
DseDI GACNNNNNNGTC 1 cut(s) 914
Eam1104I CTCTTC 2 cut(s) 252, 815
EarI CTCTTC 2 cut(s) 252, 815
Ecl136II GAGCTC 1 cut(s) 528
Eco147I AGGCCT 1 cut(s) 831
Eco24I GRGCYC 2 cut(s) 65, 530
Eco47I GGWCC 3 cut(s) 197, 203, 907
Eco53kI GAGCTC 1 cut(s) 528
Eco57I CTGAAG 2 cut(s) 792, 957
EcoICRI GAGCTC 1 cut(s) 528
EcoNI CCTNNNNNAGG 1 cut(s) 861
EcoO109I RGGNCCY 3 cut(s) 61, 62, 907
EcoRI GAATTC 1 cut(s) 352
EcoRII CCWGG 2 cut(s) 199, 831
EcoT38I GRGCYC 2 cut(s) 65, 530
Esp3I CGTCTC 1 cut(s) 922
FaeI CATG 7 cut(s) 134, 275, 295, 737, 820, 1054, 1096
FalI AAGNNNNNCTT 6 cut(s) 381, 413, 419, 451, 491, 523
FaqI GGGAC 1 cut(s) 67
FatI CATG 7 cut(s) 130, 271, 291, 733, 816, 1050, 1092
FauI CCCGC 1 cut(s) 551
FblI GTMKAC 2 cut(s) 237, 414
Fnu4HI GCNGC 4 cut(s) 14, 297, 683, 839
FokI GGATG 2 cut(s) 174, 533
FriOI GRGCYC 2 cut(s) 65, 530
Fsp4HI GCNGC 4 cut(s) 14, 297, 683, 839
GluI GCNGC 4 cut(s) 14, 297, 683, 839
HaeIII GGCC 3 cut(s) 63, 381, 831
HapII CCGG 5 cut(s) 168, 192, 637, 900, 1077
Hin1II CATG 7 cut(s) 134, 275, 295, 737, 820, 1054, 1096
HincII GTYRAC 3 cut(s) 238, 313, 415
HindII GTYRAC 3 cut(s) 238, 313, 415
HindIII AAGCTT 2 cut(s) 749, 804
HinfI GANTC 5 cut(s) 242, 394, 713, 890, 1039
HpaII CCGG 5 cut(s) 168, 192, 637, 900, 1077
HphI GGTGA 3 cut(s) 156, 914, 1034
Hpy166II GTNNAC 5 cut(s) 238, 280, 313, 415, 631
Hpy188I TCNGA 4 cut(s) 36, 712, 811, 1064
Hpy188III TCNNGA 8 cut(s) 71, 99, 131, 518, 601, 665, 717, 817
Hpy8I GTNNAC 5 cut(s) 238, 280, 313, 415, 631
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 4 cut(s) 361, 524, 671, 953
HpyCH4III ACNGT 5 cut(s) 182, 967, 985, 1073, 1084
HpyCH4IV ACGT 2 cut(s) 577, 915
HpyCH4V TGCA 2 cut(s) 440, 671
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
HpyF3I CTNAG 2 cut(s) 35, 363
HpySE526I ACGT 2 cut(s) 577, 915
Hsp92II CATG 7 cut(s) 134, 275, 295, 737, 820, 1054, 1096
Kzo9I GATC 4 cut(s) 73, 101, 161, 853
LmnI GCTCC 2 cut(s) 525, 1015
Lsp1109I GCAGC 2 cut(s) 669, 825
LweI GCATC 1 cut(s) 658
MaeII ACGT 2 cut(s) 577, 915
MaeIII GTNAC 5 cut(s) 139, 173, 503, 874, 979
MalI GATC 4 cut(s) 75, 103, 163, 855
MboI GATC 4 cut(s) 73, 101, 161, 853
MboII GAAGA 6 cut(s) 239, 389, 783, 832, 968, 1079
MflI RGATCY 1 cut(s) 101
MhlI GDGCHC 4 cut(s) 65, 530, 1012, 1107
MluCI AATT 4 cut(s) 352, 406, 621, 948
MlyI GAGTC 2 cut(s) 251, 899
MseI TTAA 5 cut(s) 471, 534, 585, 783, 861
MspA1I CMGCKG 1 cut(s) 977
MspI CCGG 5 cut(s) 168, 192, 637, 900, 1077
MspR9I CCNGG 5 cut(s) 168, 192, 201, 637, 833
MvaI CCWGG 2 cut(s) 201, 833
MwoI GCNNNNNNNGC 1 cut(s) 293
NciI CCSGG 3 cut(s) 168, 192, 637
NdeII GATC 4 cut(s) 73, 101, 161, 853
NlaIII CATG 7 cut(s) 134, 275, 295, 737, 820, 1054, 1096
NlaIV GGNNCC 2 cut(s) 63, 909
NmeAIII GCCGAG 1 cut(s) 899
NmuCI GTSAC 3 cut(s) 139, 173, 874
NspI RCATGY 1 cut(s) 275
PagI TCATGA 2 cut(s) 130, 816
PceI AGGCCT 1 cut(s) 831
PciI ACATGT 1 cut(s) 271
PcsI WCGNNNNNNNCGW 2 cut(s) 144, 153
PfeI GAWTC 3 cut(s) 394, 713, 1039
PflFI GACNNNGTC 1 cut(s) 137
PkrI GCNGC 4 cut(s) 15, 298, 684, 840
PleI GAGTC 2 cut(s) 250, 898
PpsI GAGTC 2 cut(s) 250, 898
Ppu21I YACGTR 1 cut(s) 578
PpuMI RGGWCCY 1 cut(s) 907
PscI ACATGT 1 cut(s) 271
Psp124BI GAGCTC 1 cut(s) 530
Psp5II RGGWCCY 1 cut(s) 907
Psp6I CCWGG 2 cut(s) 199, 831
PspGI CCWGG 2 cut(s) 199, 831
PspN4I GGNNCC 2 cut(s) 63, 909
PspOMI GGGCCC 1 cut(s) 61
PspPI GGNCC 5 cut(s) 61, 62, 197, 203, 907
PspPPI RGGWCCY 1 cut(s) 907
PsuI RGATCY 1 cut(s) 101
PsyI GACNNNGTC 1 cut(s) 137
PvuII CAGCTG 1 cut(s) 977
RsaI GTAC 3 cut(s) 653, 964, 1075
RsaNI GTAC 3 cut(s) 652, 963, 1074
SacI GAGCTC 1 cut(s) 530
SalI GTCGAC 2 cut(s) 236, 413
SaqAI TTAA 5 cut(s) 471, 534, 585, 783, 861
SatI GCNGC 4 cut(s) 14, 297, 683, 839
Sau3AI GATC 4 cut(s) 73, 101, 161, 853
Sau96I GGNCC 5 cut(s) 61, 62, 197, 203, 907
SchI GAGTC 2 cut(s) 251, 899
ScrFI CCNGG 5 cut(s) 168, 192, 201, 637, 833
SduI GDGCHC 4 cut(s) 65, 530, 1012, 1107
SfaNI GCATC 1 cut(s) 658
SinI GGWCC 3 cut(s) 197, 203, 907
SmlI CTYRAG 2 cut(s) 92, 601
SmoI CTYRAG 2 cut(s) 92, 601
Sse9I AATT 4 cut(s) 352, 406, 621, 948
SseBI AGGCCT 1 cut(s) 831
SsiI CCGC 4 cut(s) 14, 195, 296, 558
SspI AATATT 1 cut(s) 927
SstI GAGCTC 1 cut(s) 530
StuI AGGCCT 1 cut(s) 831
StyD4I CCNGG 5 cut(s) 166, 190, 199, 635, 831
TaaI ACNGT 5 cut(s) 182, 967, 985, 1073, 1084
TaiI ACGT 2 cut(s) 580, 918
TaqI TCGA 3 cut(s) 237, 414, 945
TasI AATT 4 cut(s) 352, 406, 621, 948
TatI WGTACW 1 cut(s) 651
TauI GCSGC 2 cut(s) 16, 299
TfiI GAWTC 3 cut(s) 394, 713, 1039
Tru1I TTAA 5 cut(s) 471, 534, 585, 783, 861
Tru9I TTAA 5 cut(s) 471, 534, 585, 783, 861
TscAI CASTG 3 cut(s) 178, 289, 1089
TseFI GTSAC 3 cut(s) 139, 173, 874
TseI GCWGC 2 cut(s) 682, 838
Tsp45I GTSAC 3 cut(s) 139, 173, 874
TspDTI ATGAA 7 cut(s) 176, 833, 920, 969, 1039, 1048, 1081
TspRI CASTG 3 cut(s) 178, 289, 1089
Tth111I GACNNNGTC 1 cut(s) 137
VpaK11BI GGWCC 3 cut(s) 197, 203, 907
XagI CCTNNNNNAGG 1 cut(s) 861
XapI RAATTY 2 cut(s) 352, 406
XceI RCATGY 1 cut(s) 275
XmiI GTMKAC 2 cut(s) 237, 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.