MD03G1006100.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015505
Physical Location & Seq
Forward (+)
481575 .. 484352
2778 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1006100.v1.1.491

Sequence Viewer

Length: 510 bp
ATGACGACACCGTCAGCACCGTCCTTGGCATCGTCGTCGAGGTGGGCGTCGACGACCTCAGTGTCGTCCTCAGGGAAGAGGATTCAGAGAGAAATGGTGGAGCTTAACATGGACCCACCTCCCGACTGCTGCGCTGGGCCCAAGGGCGACAATCTCTACCACTGGATCGCCACCCTTTTTGGCCCCCCAGGAACACCGTACCACGGTGGCATATATTTCCTTGACATTACGTTTCCAAGTGACTATCCGTTTAAACCTCCAAAGGTTGTATTCACAACTCGAATTTATCATTGCAATGTTGATTCTAACGGAAATCTAAGCATGGAAATGTTAAAGGATAGTTGGAGTCCAGCTCTAACAATAACCAAGTTGCTACTAGCCATCCGATCCATCTTCACAAGACCTGATCCTTATAATCCCCTTGTCCCCAGCATTGCCCGTTTGTACTTGGAGGATAAAGCTAAACATGATGAACTTGCTTCTGAGTGGACGCTGCGATTTGCTAAGTGA

Protein Analysis

170

Amino Acids

18.88

Weight (kDa)

7.72

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 27 - 163 8.8e-42 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13550 AT3G13550
fragaria_vesca FvH4_3g45321 FvH4_3g45321
malus_domestica MD03G1006100.v1.1
prunus_persica Prupe.6G005800_v2.0.a1
pyrus_communis pycom03g00610
rosa_chinensis RchiOBHm_Chr5g0083101
rosa_laevigata RLG00000037011
rosa_rugosa Rorug05G0482200 Rorug05G0482200
rosa_samantha Rh5AG531900 Rh5BG559000 Rh5CG581900 Rh5DG567800
rosa_wichuraiana Rw5G049650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
AasI GACNNNNNNGTC 2 cut(s) 10, 61
AccI GTMKAC 1 cut(s) 50
AclWI GGATC 3 cut(s) 173, 381, 401
AcsI RAATTY 1 cut(s) 282
AcyI GRCGYC 1 cut(s) 47
AfaI GTAC 2 cut(s) 200, 446
AfiI CCNNNNNNNGG 1 cut(s) 203
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 3 cut(s) 103, 353, 461
AluI AGCT 3 cut(s) 103, 353, 461
AlwI GGATC 3 cut(s) 173, 381, 401
AoxI GGCC 2 cut(s) 137, 181
ApaI GGGCCC 1 cut(s) 141
ApeKI GCWGC 2 cut(s) 129, 493
ApoI RAATTY 1 cut(s) 282
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 4 cut(s) 112, 137, 138, 182
AvaII GGWCC 1 cut(s) 112
AxyI CCTNAGG 1 cut(s) 70
BaeGI GKGCMC 1 cut(s) 141
BanII GRGCYC 1 cut(s) 141
BbvI GCAGC 2 cut(s) 116, 480
BccI CCATC 2 cut(s) 389, 398
BcgI CGANNNNNNTGC 2 cut(s) 18, 52
BciT130I CCWGG 1 cut(s) 189
BfaI CTAG 1 cut(s) 377
BisI GCNGC 2 cut(s) 130, 494
BlsI GCNGC 2 cut(s) 131, 495
Bme1390I CCNGG 1 cut(s) 189
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 4 cut(s) 112, 137, 138, 182
BmiI GGNNCC 3 cut(s) 114, 139, 184
BmrFI CCNGG 1 cut(s) 189
BmsI GCATC 1 cut(s) 38
BplI GAGNNNNNCTC 2 cut(s) 337, 369
BsaHI GRCGYC 1 cut(s) 47
BsaJI CCNNGG 4 cut(s) 24, 141, 187, 202
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 167
Bse21I CCTNAGG 1 cut(s) 70
Bse3DI GCAATG 3 cut(s) 289, 301, 432
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 4 cut(s) 24, 141, 187, 202
BseGI GGATG 1 cut(s) 381
BseLI CCNNNNNNNGG 1 cut(s) 203
BseMI GCAATG 3 cut(s) 289, 301, 432
BseMII CTCAG 3 cut(s) 72, 84, 474
BseNI ACTGG 1 cut(s) 167
BseSI GKGCMC 1 cut(s) 141
BseXI GCAGC 2 cut(s) 116, 480
BseYI CCCAGC 2 cut(s) 134, 428
BshFI GGCC 2 cut(s) 139, 183
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 1 cut(s) 203
BsmFI GGGAC 1 cut(s) 410
BsnI GGCC 2 cut(s) 139, 183
Bsp120I GGGCCC 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 141
Bsp143I GATC 3 cut(s) 165, 386, 406
BspANI GGCC 2 cut(s) 139, 183
BspCNI CTCAG 3 cut(s) 71, 83, 475
BspLI GGNNCC 3 cut(s) 114, 139, 184
BspPI GGATC 3 cut(s) 173, 381, 401
BsrDI GCAATG 3 cut(s) 289, 301, 432
BsrI ACTGG 1 cut(s) 167
BssECI CCNNGG 4 cut(s) 24, 141, 187, 202
BssMI GATC 3 cut(s) 165, 386, 406
BssNI GRCGYC 1 cut(s) 47
BssT1I CCWWGG 2 cut(s) 24, 141
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 4 cut(s) 12, 21, 198, 206
Bst6I CTCTTC 1 cut(s) 71
BstACI GRCGYC 1 cut(s) 47
BstDEI CTNAG 5 cut(s) 58, 70, 317, 483, 504
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 1 cut(s) 381
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 3 cut(s) 168, 389, 409
BstMBI GATC 3 cut(s) 165, 386, 406
BstNI CCWGG 1 cut(s) 189
BstSCI CCNGG 1 cut(s) 187
BstSLI GKGCMC 1 cut(s) 141
BstV1I GCAGC 2 cut(s) 116, 480
Bsu36I CCTNAGG 1 cut(s) 70
BsuRI GGCC 2 cut(s) 139, 183
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 1 cut(s) 381
BtsIMutI CAGTG 2 cut(s) 66, 160
CfoI GCGC 1 cut(s) 134
Cfr13I GGNCC 4 cut(s) 112, 137, 138, 182
CseI GACGC 2 cut(s) 36, 499
Csp6I GTAC 2 cut(s) 199, 445
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviAII CATG 3 cut(s) 109, 322, 467
CviJI RGCY 6 cut(s) 103, 139, 183, 353, 380, 461
CviKI_1 RGCY 6 cut(s) 103, 139, 183, 353, 380, 461
CviQI GTAC 2 cut(s) 199, 445
DdeI CTNAG 5 cut(s) 58, 70, 317, 483, 504
DpnI GATC 3 cut(s) 167, 388, 408
DpnII GATC 3 cut(s) 165, 386, 406
DraI TTTAAA 1 cut(s) 253
DrdI GACNNNNNNGTC 2 cut(s) 10, 61
DseDI GACNNNNNNGTC 2 cut(s) 10, 61
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco130I CCWWGG 2 cut(s) 24, 141
Eco24I GRGCYC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 112
Eco81I CCTNAGG 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 2 cut(s) 24, 141
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 2 cut(s) 24, 141
FaeI CATG 3 cut(s) 112, 325, 470
FaiI YATR 6 cut(s) 110, 212, 214, 323, 414, 468
FaqI GGGAC 1 cut(s) 410
FatI CATG 3 cut(s) 108, 321, 466
FblI GTMKAC 1 cut(s) 50
Fnu4HI GCNGC 2 cut(s) 130, 494
FokI GGATG 1 cut(s) 368
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 2 cut(s) 130, 494
FspBI CTAG 1 cut(s) 377
GlaI GCGC 1 cut(s) 133
GluI GCNGC 2 cut(s) 130, 494
GsaI CCCAGC 2 cut(s) 138, 432
HaeIII GGCC 2 cut(s) 139, 183
HgaI GACGC 2 cut(s) 36, 499
HhaI GCGC 1 cut(s) 134
Hin1I GRCGYC 1 cut(s) 47
Hin1II CATG 3 cut(s) 112, 325, 470
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HinfI GANTC 3 cut(s) 82, 302, 346
Hpy166II GTNNAC 2 cut(s) 51, 489
Hpy188I TCNGA 3 cut(s) 87, 386, 484
Hpy188III TCNNGA 1 cut(s) 122
Hpy8I GTNNAC 2 cut(s) 51, 489
Hpy99I CGWCG 4 cut(s) 37, 40, 52, 55
HpyCH4III ACNGT 4 cut(s) 12, 21, 198, 206
HpyCH4IV ACGT 1 cut(s) 230
HpyCH4V TGCA 1 cut(s) 294
HpyF3I CTNAG 5 cut(s) 58, 70, 317, 483, 504
HpySE526I ACGT 1 cut(s) 230
Hsp92I GRCGYC 1 cut(s) 47
Hsp92II CATG 3 cut(s) 112, 325, 470
HspAI GCGC 1 cut(s) 132
Kzo9I GATC 3 cut(s) 165, 386, 406
LmnI GCTCC 1 cut(s) 100
LpnPI CCDG 8 cut(s) 57, 120, 148, 174, 201, 363, 417, 442
Lsp1109I GCAGC 2 cut(s) 116, 480
LweI GCATC 1 cut(s) 38
MaeI CTAG 1 cut(s) 377
MaeII ACGT 1 cut(s) 230
MaeIII GTNAC 1 cut(s) 239
MalI GATC 3 cut(s) 167, 388, 408
MboI GATC 3 cut(s) 165, 386, 406
MboII GAAGA 2 cut(s) 88, 385
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 1 cut(s) 282
MlyI GAGTC 1 cut(s) 355
MmeI TCCRAC 1 cut(s) 323
MnlI CCTC 7 cut(s) 33, 67, 72, 79, 129, 267, 445
MseI TTAA 3 cut(s) 105, 252, 332
MslI CAYNNNNRTG 2 cut(s) 294, 326
MspR9I CCNGG 1 cut(s) 189
MssI GTTTAAAC 1 cut(s) 253
MvaI CCWGG 1 cut(s) 189
NdeII GATC 3 cut(s) 165, 386, 406
NlaIII CATG 3 cut(s) 112, 325, 470
NlaIV GGNNCC 3 cut(s) 114, 139, 184
NmuCI GTSAC 1 cut(s) 239
PcsI WCGNNNNNNNCGW 1 cut(s) 44
PfeI GAWTC 2 cut(s) 82, 302
PflFI GACNNNGTC 1 cut(s) 10
PkrI GCNGC 2 cut(s) 131, 495
PleI GAGTC 1 cut(s) 354
PmeI GTTTAAAC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 354
PsiI TTATAA 1 cut(s) 414
Psp6I CCWGG 1 cut(s) 187
PspFI CCCAGC 2 cut(s) 134, 428
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 3 cut(s) 114, 139, 184
PspOMI GGGCCC 1 cut(s) 137
PspPI GGNCC 4 cut(s) 112, 137, 138, 182
PsyI GACNNNGTC 1 cut(s) 10
RsaI GTAC 2 cut(s) 200, 446
RsaNI GTAC 2 cut(s) 199, 445
RseI CAYNNNNRTG 2 cut(s) 294, 326
SalI GTCGAC 1 cut(s) 49
SaqAI TTAA 3 cut(s) 105, 252, 332
SatI GCNGC 2 cut(s) 130, 494
Sau3AI GATC 3 cut(s) 165, 386, 406
Sau96I GGNCC 4 cut(s) 112, 137, 138, 182
SchI GAGTC 1 cut(s) 355
ScrFI CCNGG 1 cut(s) 189
SduI GDGCHC 1 cut(s) 141
SfaNI GCATC 1 cut(s) 38
SgrDI CGTCGACG 1 cut(s) 49
SinI GGWCC 1 cut(s) 112
SmiMI CAYNNNNRTG 2 cut(s) 294, 326
Sse9I AATT 1 cut(s) 282
SspMI CTAG 1 cut(s) 377
StyD4I CCNGG 1 cut(s) 187
StyI CCWWGG 2 cut(s) 24, 141
TaaI ACNGT 4 cut(s) 12, 21, 198, 206
TaiI ACGT 1 cut(s) 233
TaqI TCGA 3 cut(s) 38, 50, 280
TasI AATT 1 cut(s) 282
TatI WGTACW 1 cut(s) 444
TfiI GAWTC 2 cut(s) 82, 302
Tru1I TTAA 3 cut(s) 105, 252, 332
Tru9I TTAA 3 cut(s) 105, 252, 332
TscAI CASTG 2 cut(s) 66, 167
TseFI GTSAC 1 cut(s) 239
TseI GCWGC 2 cut(s) 129, 493
Tsp45I GTSAC 1 cut(s) 239
TspDTI ATGAA 1 cut(s) 486
TspGWI ACGGA 2 cut(s) 237, 324
TspRI CASTG 2 cut(s) 66, 167
Tth111I GACNNNGTC 1 cut(s) 10
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 1 cut(s) 282
XmiI GTMKAC 1 cut(s) 50
XspI CTAG 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.