RchiOBHm_Chr5g0083101

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0015505
Physical Location & Seq
Reverse (-)
88814521 .. 88817110
2590 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35726

Sequence Viewer

Length: 498 bp
ATGACAACATCGTCGGCATCAACCTCTACGTGGGTGTCCACGACCTCTGTGTCTTCCTCAGGGAAGAGAATCCAGAGAGAAATGGTTGAGCTCAACATGGACCCACCTCCTGATTGCTCTGCTGGGCCCAAGGGTGACAATCTCTACCACTGGGTTGCCACCATCATTGGCCCTCCAGGAACACCATACCAAGGTGGCATATACTTCCTTGACATTACATTTCCTGGTGATTATCCATTTAAACCTCCAAAGATCGTATTCAGGACTCGAGTCTTTCATTGCAATGTCGATTCTTCTGGTAATGTAAGTTTGGAAATCGTAAAGGATGGTTGGAGTCCAGCTCTGACAATTACAAAGGTGCTACTAGCAATTCGGTCAATTTTCACAAAACCTGATCCTTATAATCCGCTTGTCCCTAGCATCGCTCATTTGTACTTGGCAGATAAAGTTAAACACGATGAACTAGCTTCTGAGTGGACAATGCGATTCGCCAAGTGA

Protein Analysis

165

Amino Acids

18.23

Weight (kDa)

7.78

Isoelectric Point (pI)

24.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 23 - 159 1.1e-42 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0015505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13550 AT3G13550
fragaria_vesca FvH4_3g45321 FvH4_3g45321
malus_domestica MD03G1006100.v1.1
prunus_persica Prupe.6G005800_v2.0.a1
pyrus_communis pycom03g00610
rosa_chinensis RchiOBHm_Chr5g0083101
rosa_laevigata RLG00000037011
rosa_rugosa Rorug05G0482200 Rorug05G0482200
rosa_samantha Rh5AG531900 Rh5BG559000 Rh5CG581900 Rh5DG567800
rosa_wichuraiana Rw5G049650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 402
AasI GACNNNNNNGTC 2 cut(s) 10, 49
AciI CCGC 1 cut(s) 407
AclWI GGATC 1 cut(s) 389
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 2 cut(s) 30, 191
AjnI CCWGG 2 cut(s) 175, 223
AluBI AGCT 3 cut(s) 91, 341, 467
AluI AGCT 3 cut(s) 91, 341, 467
Alw21I GWGCWC 1 cut(s) 93
AlwI GGATC 1 cut(s) 389
Ama87I CYCGRG 1 cut(s) 267
AoxI GGCC 2 cut(s) 125, 169
ApaI GGGCCC 1 cut(s) 129
AspS9I GGNCC 4 cut(s) 100, 125, 126, 170
AsuHPI GGTGA 2 cut(s) 146, 239
AvaI CYCGRG 1 cut(s) 267
AvaII GGWCC 1 cut(s) 100
AxyI CCTNAGG 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 129
BanII GRGCYC 2 cut(s) 93, 129
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 93
BccI CCATC 2 cut(s) 170, 320
BciT130I CCWGG 2 cut(s) 177, 225
BfaI CTAG 3 cut(s) 365, 417, 464
Bme1390I CCNGG 2 cut(s) 177, 225
Bme18I GGWCC 1 cut(s) 100
BmeT110I CYCGRG 1 cut(s) 267
BmgT120I GGNCC 4 cut(s) 100, 125, 126, 170
BmiI GGNNCC 2 cut(s) 102, 127
BmrFI CCNGG 2 cut(s) 177, 225
BmrI ACTGGG 1 cut(s) 160
BmsI GCATC 2 cut(s) 26, 429
BmuI ACTGGG 1 cut(s) 160
BoxI GACNNNNGTC 1 cut(s) 269
BpiI GAAGAC 1 cut(s) 45
BplI GAGNNNNNCTC 2 cut(s) 325, 357
BpmI CTGGAG 1 cut(s) 159
BsaAI YACGTR 1 cut(s) 30
BsaJI CCNNGG 2 cut(s) 129, 190
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 191
Bse1I ACTGG 1 cut(s) 155
Bse21I CCTNAGG 1 cut(s) 58
Bse3DI GCAATG 2 cut(s) 277, 289
BseBI CCWGG 2 cut(s) 177, 225
BseDI CCNNGG 2 cut(s) 129, 190
BseGI GGATG 1 cut(s) 331
BseLI CCNNNNNNNGG 2 cut(s) 30, 191
BseMI GCAATG 2 cut(s) 277, 289
BseMII CTCAG 2 cut(s) 72, 462
BseNI ACTGG 1 cut(s) 155
BseSI GKGCMC 1 cut(s) 129
BseYI CCCAGC 1 cut(s) 122
BshFI GGCC 2 cut(s) 127, 171
BsiHKAI GWGCWC 1 cut(s) 93
BsiHKCI CYCGRG 1 cut(s) 267
BslFI GGGAC 1 cut(s) 398
BslI CCNNNNNNNGG 2 cut(s) 30, 191
BsmFI GGGAC 1 cut(s) 398
BsnI GGCC 2 cut(s) 127, 171
BsoBI CYCGRG 1 cut(s) 267
Bsp120I GGGCCC 1 cut(s) 125
Bsp1286I GDGCHC 2 cut(s) 93, 129
Bsp143I GATC 2 cut(s) 252, 394
BspACI CCGC 1 cut(s) 407
BspANI GGCC 2 cut(s) 127, 171
BspCNI CTCAG 2 cut(s) 71, 463
BspLI GGNNCC 2 cut(s) 102, 127
BspPI GGATC 1 cut(s) 389
BsrDI GCAATG 2 cut(s) 277, 289
BsrI ACTGG 1 cut(s) 155
BssECI CCNNGG 2 cut(s) 129, 190
BssMI GATC 2 cut(s) 252, 394
BssT1I CCWWGG 2 cut(s) 129, 190
Bst2UI CCWGG 2 cut(s) 177, 225
Bst6I CTCTTC 1 cut(s) 59
BstBAI YACGTR 1 cut(s) 30
BstDEI CTNAG 2 cut(s) 58, 471
BstF5I GGATG 1 cut(s) 331
BstKTI GATC 2 cut(s) 255, 397
BstMBI GATC 2 cut(s) 252, 394
BstNI CCWGG 2 cut(s) 177, 225
BstPAI GACNNNNGTC 1 cut(s) 269
BstSCI CCNGG 2 cut(s) 175, 223
BstSLI GKGCMC 1 cut(s) 129
BstV2I GAAGAC 1 cut(s) 45
Bsu36I CCTNAGG 1 cut(s) 58
BsuRI GGCC 2 cut(s) 127, 171
BtgZI GCGATG 1 cut(s) 406
BtsCI GGATG 1 cut(s) 331
BtsIMutI CAGTG 1 cut(s) 148
Cfr13I GGNCC 4 cut(s) 100, 125, 126, 170
Csp6I GTAC 1 cut(s) 433
CspCI CAANNNNNGTGG 2 cut(s) 148, 183
CviAII CATG 1 cut(s) 97
CviJI RGCY 5 cut(s) 91, 127, 171, 341, 467
CviKI_1 RGCY 5 cut(s) 91, 127, 171, 341, 467
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 2 cut(s) 58, 471
DpnI GATC 2 cut(s) 254, 396
DpnII GATC 2 cut(s) 252, 394
DraI TTTAAA 1 cut(s) 241
DrdI GACNNNNNNGTC 2 cut(s) 10, 49
DseDI GACNNNNNNGTC 2 cut(s) 10, 49
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 91
Eco130I CCWWGG 2 cut(s) 129, 190
Eco24I GRGCYC 2 cut(s) 93, 129
Eco47I GGWCC 1 cut(s) 100
Eco53kI GAGCTC 1 cut(s) 91
Eco81I CCTNAGG 1 cut(s) 58
Eco88I CYCGRG 1 cut(s) 267
EcoICRI GAGCTC 1 cut(s) 91
EcoRII CCWGG 2 cut(s) 175, 223
EcoT14I CCWWGG 2 cut(s) 129, 190
EcoT38I GRGCYC 2 cut(s) 93, 129
ErhI CCWWGG 2 cut(s) 129, 190
FaeI CATG 1 cut(s) 100
FaiI YATR 5 cut(s) 98, 187, 200, 202, 402
FaqI GGGAC 1 cut(s) 398
FatI CATG 1 cut(s) 96
FokI GGATG 1 cut(s) 338
FriOI GRGCYC 2 cut(s) 93, 129
FspBI CTAG 3 cut(s) 365, 417, 464
GsaI CCCAGC 1 cut(s) 126
GsuI CTGGAG 1 cut(s) 159
HaeIII GGCC 2 cut(s) 127, 171
Hin1II CATG 1 cut(s) 100
HinfI GANTC 6 cut(s) 69, 265, 270, 290, 334, 486
HphI GGTGA 2 cut(s) 146, 239
Hpy166II GTNNAC 2 cut(s) 39, 477
Hpy188I TCNGA 2 cut(s) 345, 472
Hpy188III TCNNGA 3 cut(s) 73, 110, 262
Hpy8I GTNNAC 2 cut(s) 39, 477
Hpy99I CGWCG 1 cut(s) 16
HpyCH4IV ACGT 1 cut(s) 29
HpyCH4V TGCA 1 cut(s) 282
HpyF3I CTNAG 2 cut(s) 58, 471
HpySE526I ACGT 1 cut(s) 29
Hsp92II CATG 1 cut(s) 100
Kzo9I GATC 2 cut(s) 252, 394
LweI GCATC 2 cut(s) 26, 429
MaeI CTAG 3 cut(s) 365, 417, 464
MaeII ACGT 1 cut(s) 29
MaeIII GTNAC 1 cut(s) 134
MalI GATC 2 cut(s) 254, 396
MboI GATC 2 cut(s) 252, 394
MboII GAAGA 3 cut(s) 45, 76, 285
MhlI GDGCHC 2 cut(s) 93, 129
MluCI AATT 3 cut(s) 348, 369, 378
MlyI GAGTC 3 cut(s) 259, 279, 343
MmeI TCCRAC 1 cut(s) 311
MnlI CCTC 6 cut(s) 34, 55, 67, 117, 183, 255
MseI TTAA 2 cut(s) 240, 450
MslI CAYNNNNRTG 1 cut(s) 282
MspR9I CCNGG 2 cut(s) 177, 225
MvaI CCWGG 2 cut(s) 177, 225
NdeII GATC 2 cut(s) 252, 394
NlaIII CATG 1 cut(s) 100
NlaIV GGNNCC 2 cut(s) 102, 127
NmuCI GTSAC 1 cut(s) 134
PaeR7I CTCGAG 1 cut(s) 267
PfeI GAWTC 3 cut(s) 69, 290, 486
PfoI TCCNGGA 1 cut(s) 175
PleI GAGTC 3 cut(s) 259, 278, 342
PpsI GAGTC 3 cut(s) 259, 278, 342
Ppu21I YACGTR 1 cut(s) 30
PshAI GACNNNNGTC 1 cut(s) 269
PsiI TTATAA 1 cut(s) 402
Psp124BI GAGCTC 1 cut(s) 93
Psp6I CCWGG 2 cut(s) 175, 223
PspFI CCCAGC 1 cut(s) 122
PspGI CCWGG 2 cut(s) 175, 223
PspN4I GGNNCC 2 cut(s) 102, 127
PspOMI GGGCCC 1 cut(s) 125
PspPI GGNCC 4 cut(s) 100, 125, 126, 170
PspXI VCTCGAGB 1 cut(s) 267
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 1 cut(s) 282
SacI GAGCTC 1 cut(s) 93
SaqAI TTAA 2 cut(s) 240, 450
Sau3AI GATC 2 cut(s) 252, 394
Sau96I GGNCC 4 cut(s) 100, 125, 126, 170
SchI GAGTC 3 cut(s) 259, 279, 343
ScrFI CCNGG 2 cut(s) 177, 225
SduI GDGCHC 2 cut(s) 93, 129
SfaNI GCATC 2 cut(s) 26, 429
Sfr274I CTCGAG 1 cut(s) 267
SinI GGWCC 1 cut(s) 100
SlaI CTCGAG 1 cut(s) 267
SmiMI CAYNNNNRTG 1 cut(s) 282
SmlI CTYRAG 1 cut(s) 267
SmoI CTYRAG 1 cut(s) 267
Sse9I AATT 3 cut(s) 348, 369, 378
SsiI CCGC 1 cut(s) 407
SspMI CTAG 3 cut(s) 365, 417, 464
SstI GAGCTC 1 cut(s) 93
StyD4I CCNGG 2 cut(s) 175, 223
StyI CCWWGG 2 cut(s) 129, 190
TaiI ACGT 1 cut(s) 32
TaqI TCGA 2 cut(s) 268, 288
TaqII GACCGA 1 cut(s) 363
TasI AATT 3 cut(s) 348, 369, 378
TatI WGTACW 1 cut(s) 432
TfiI GAWTC 3 cut(s) 69, 290, 486
Tru1I TTAA 2 cut(s) 240, 450
Tru9I TTAA 2 cut(s) 240, 450
TscAI CASTG 1 cut(s) 155
TseFI GTSAC 1 cut(s) 134
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 2 cut(s) 266, 474
TspRI CASTG 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 100
XhoI CTCGAG 1 cut(s) 267
XspI CTAG 3 cut(s) 365, 417, 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.